LOCUS       DBNGSB010000001         6906 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_1842469, whole genome shotgun sequence.
ACCESSION   DBNGSB010000001 DBNGSB010000000
VERSION     DBNGSB010000001.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 6906)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6906)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6906
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_1842469"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="AC159B_00005"
     CDS             <1..391
                     /locus_tag="AC159B_00005"
                     /inference="COORDINATES: protein motif:HMM:NF015445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 2 periplasmic-binding domain-containing
                     protein"
                     /protein_id="HIR3376169.1"
                     /translation="GVMDGQENPLAQIWGAKFHEVQRFLSMTGHVYTPAYPVAGERWW
                     QTVPPDLRIAIRRIAIEVGDYARQHGERLDNSLVAEMQKANPRLRVNDVDKAAFIAAS
                     KAIYDDFSKEVPNGAALIRLIQGLAPR"
     gene            399..917
                     /locus_tag="AC159B_00010"
     CDS             399..917
                     /locus_tag="AC159B_00010"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR3376170.1"
                     /translation="MLPAQVTAPAVRTSGFFRTVGTIVSILEVFCAVMMVAMAVIVLL
                     GVFYRYVIQRALPWYDEFAEYLLVWLTFYGSILAAHRGAHIGFDTLVEMMPPAVQRVV
                     MLIGEAAMLLIQLALFVHGWRLAEAASFDAAVSIRWIRLSWVYSAIPIGGAFMFLVSL
                     RRIAALWMTPRE"
     gene            1012..2295
                     /locus_tag="AC159B_00015"
     CDS             1012..2295
                     /locus_tag="AC159B_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR3376171.1"
                     /translation="MNAGIVIGIIILLLILNVPISFALIGGVLAALLVGGGTDLAVIP
                     LQLFSGSAKFPLLAIPLFILAGGLMNSSGISLRIINLASALVGHVRGGLAMVNVVDSM
                     FFAEISGSAVADVAALGSIITPAMVKKGYKPAFTAALTSVTASLAIIIPPSIPMILYA
                     VISGTSVTRLFLTGVAPGVIGGLLMMAVVYYKARRYGFPVEEAFSFRRLGGVFREAGW
                     TLALPVIILGGMFGGIVTATEAAGLAVLAAFIIGTFIYREMNFKQLWSVTLDGVTSTA
                     SVMMIVAGSAVLGWYLTNEQVPQQFAAAVLGITTDPVLVLLFLNIFLLVAGMFLHSAA
                     AILLIVPIVMPLIRQVGVDPTHFGIIVTLNLAIGQQTPPVASVLLTACAVAKQPVGKV
                     FWEAIPFIIALLITLAIVTYVPAIPMFLPRVLQGP"
     gene            2393..2587
                     /locus_tag="AC159B_00020"
                     /pseudo
     CDS             2393..2587
                     /locus_tag="AC159B_00020"
                     /inference="COORDINATES: protein motif:HMM:NF021758.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rdx family protein"
     gene            complement(2577..3014)
                     /locus_tag="AC159B_00025"
     CDS             complement(2577..3014)
                     /locus_tag="AC159B_00025"
                     /inference="COORDINATES: protein motif:HMM:NF024970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3376172.1"
                     /translation="MHNGPTGTMAWTLTIRVYSEPTAMRTVRKLVNSAARLAGASEAD
                     AYDLEVAVGEAVTNAYLHAYGGKTEGQIDVDLGFNGKDFLVSVHDGGKPVTDRITIPE
                     TPPESGHGRGLFLIGQLMDEVTIVQPDRGGQGVAVRMSKNLTG"
     gene            3144..4340
                     /locus_tag="AC159B_00030"
     CDS             3144..4340
                     /locus_tag="AC159B_00030"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HIR3376173.1"
                     /translation="MPGRHFLQIPGPTNVPDRILGAMHRPVPDHRGPELPALLSEIVA
                     GLKKIFQTTRGEIVLFPVSGTGGLEASIVNTLSQGDLVLAFNIGHFSHSYAETARGFG
                     MQVDEVELPWGQGVPADLVHDRLMRDTARNYKAILMVHNETSTGVASDVRAVREAINS
                     AHHPALLLVDTVSSLASIEFLFDEWGVDVAVCGTQKGLMLPPGMAVVCAGPRAIAASA
                     QVTTPRFFFDWRPVLAEMKRGYFPFTPATLMLYGLREAVRMLLEEGLPAVFGRHRNLA
                     EGVRRAVRAWNLPILCRNSAEYSNSLTAVELPDGIDADKVLQIAQEELNLSLGGGLGR
                     LKGRVIRIGHLGALNELEVLATVAGTEMALQMAGIRIDPGVGVRACQQWFMQGYRAGT
                     RVGSRR"
     gene            4467..4865
                     /locus_tag="AC159B_00035"
     CDS             4467..4865
                     /locus_tag="AC159B_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376174.1"
                     /translation="MIHDTNVKADTFKQRWSSLVPKIRDRYPNLSDQDLSDINGDLDK
                     LVAKVSEKHGQSKAEIMNDLTTLISGPEKTVAGNTGSKMGSGSSDQYQQKEGFPKQGV
                     EQKQGQKPDFGSEGSVKEGSEREESTGKKR"
     gene            4934..5251
                     /locus_tag="AC159B_00040"
     CDS             4934..5251
                     /locus_tag="AC159B_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIR3376175.1"
                     /translation="MARYARLASGLWILMILFASLLPGGTTRAAGTGWHIIGYGVLVV
                     LLAAWQPIVRAAVAAWSLGAVVEGLQLLVAYRVAESGYLVANAAGVALGLALRAGYFA
                     FAR"
     gene            complement(5230..6078)
                     /locus_tag="AC159B_00045"
     CDS             complement(5230..6078)
                     /locus_tag="AC159B_00045"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3376176.1"
                     /translation="MPALHETRIQDNLFVWTIGGDTIETSYGANCIGVIGTDAVLLVD
                     PLIAPAHARLVDEALKDLTRAPVRFVVLTHHHTDHALGSSYFAARDAVVFSHRACREA
                     MAEEHPGIIQQRRARPELRELFADAESVLPSVTFDEGLVLHVGGIEIEVWHPGWGHTP
                     GDAFLFIPALGVAISGDLVFSGYHYNYEQASIAGARKGLQALASLDAETFIPGHGAPG
                     GPGALDAQSRYHDAVEDIVSRGLSAGWEETLITAEIARSFPDHLMSIVIPSGIKQIKA
                     HLAKAK"
     gene            complement(6062..>6906)
                     /locus_tag="AC159B_00050"
     CDS             complement(6062..>6906)
                     /locus_tag="AC159B_00050"
                     /inference="COORDINATES: protein motif:HMM:NF017807.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3376177.1"
                     /translation="VGEIGPLTLVAYRLLFGLIGLLGVLRLQRKRLPLGRAQLPAYLL
                     MGLFQAMLPISLISWGETRIDSGLAAILNGTVPLFAIVISHYWLTDEKMTAMRVVGLI
                     VGFAGVVVLVSDDFRPGALSGSLLGQLAVLAAAASYGMAATFSRRYLRNQPPVLQGTM
                     VLLTGDAFAWLAVLGLERPIQTPALPITWVAILWLGLLGSCVAYLLFFYLINSWGPTR
                     ASVVTYVFPIIGLLLGIVFLNERPDWRLYAGTALIVAGIVVLNVLGGRGLRNQRIPGH
                     ARPA"
BASE COUNT         1294 a         2054 c         2185 g         1373 t
ORIGIN      
        1 cggcgtgatg gatggacagg agaatccgct cgcgcagatc tggggcgcga agtttcatga
       61 agtgcagcgg tttctctcaa tgaccgggca cgtctacact ccggcgtatc ccgtagccgg
      121 tgagcgatgg tggcagactg tgccgcccga cctgcgcatc gcgattcgcc gcattgcgat
      181 cgaagtcggt gactatgccc gccagcatgg cgaacggttg gacaactcac tcgtggccga
      241 gatgcagaag gcgaaccccc gcttgcgcgt caatgacgtc gacaaagcgg cattcatcgc
      301 agcgtcaaag gcgatttatg acgacttttc aaaggaagtg cccaacggcg ccgccctgat
      361 tcgcttgatt caaggtctgg cgccccggta gcgactccgt gctgccggcg caagtgaccg
      421 cccccgccgt gcggacctcg gggttcttcc gcacggtggg gacgattgtt tctatcctcg
      481 aagtgttctg cgccgtcatg atggtggcca tggcggtgat cgttctgctc ggagtcttct
      541 atcggtacgt gatccagcgc gcgctgccgt ggtacgatga gttcgcggag tacctgcttg
      601 tctggctcac gttctatggg tccatcctcg ctgcccatcg gggcgcgcat atcggcttcg
      661 atacgctggt cgagatgatg ccgcccgccg tgcagcgtgt cgtgatgctg atcggtgagg
      721 ccgccatgct cctgattcag ctcgcgctgt tcgtccacgg atggcgtctc gcggaagccg
      781 cctcgtttga cgccgcggtg tcgatccgct ggatacgcct atcgtgggtg tacagcgcga
      841 ttcctattgg cggcgcgttc atgtttctcg tcagcctgag gcggatcgcc gcgctctgga
      901 tgaccccccg tgagtaatga cgaccactat agccgcccac ggagtgggcc aatggcgcac
      961 agaggccgag ctgcagcgag gccgagggaa tcctgcttgg tgccgggtta aatgaacgcc
     1021 ggcatcgtca tcggcatcat catcctgctg ctcatcctca acgtgccgat cagctttgcc
     1081 ctcatcggtg gcgtgctggc ggcgctgctc gtgggcggcg gcactgatct ggccgtcatt
     1141 ccgctgcagt tgttctcggg ctcggccaag tttccgctgc tcgccattcc gctcttcatc
     1201 ctggccggcg ggctcatgaa ctcgtcgggg atctcgcttc ggattatcaa cctggcctcg
     1261 gcgctggtcg ggcacgttcg tggcgggctc gccatggtca acgttgtgga ctcgatgttc
     1321 ttcgcggaga tctccgggtc cgcggtcgcc gacgtggcgg cgcttggcag catcatcacc
     1381 cccgccatgg tgaagaaagg ctataagccg gcgtttaccg cggcgctgac gtcggtgacg
     1441 gcgtcgctgg cgatcatcat cccgccgagc atcccgatga tcctctacgc ggtgatttcg
     1501 gggacatcgg tgacgcggct gttcctgacg ggagtcgcac caggagtgat cggtggtctc
     1561 ctgatgatgg ccgtggtgta ctacaaggcc cgccggtacg gttttccggt tgaagaggcc
     1621 ttctcattcc gacggcttgg cggcgtgttc cgagaagcgg gctggacgct ggcgctgccg
     1681 gtaatcatcc tcggcggcat gttcggaggt attgtgaccg cgaccgaggc ggcgggtctt
     1741 gcggtgctcg cggcgttcat catcggcacg tttatctaca gagaaatgaa cttcaagcag
     1801 ctctggtccg tgacgctgga cggtgtgacc tcgaccgcgt ctgtgatgat gatcgtggcc
     1861 ggatcggcgg tactcggatg gtacctgacc aacgaacagg tgccgcagca gttcgccgcg
     1921 gccgttcttg ggattactac cgaccccgtg ctcgtgctgc tcttcttgaa catcttcctg
     1981 ctcgtcgcgg ggatgttctt gcacagcgcg gccgcgatcc tgctgatcgt gccgatcgtc
     2041 atgccgctca tccgccaggt cggcgtcgat cccacccact tcgggatcat cgtgaccttg
     2101 aacctcgcca tcgggcagca gaccccgccg gtcgcctctg tgttgctcac ggcctgtgcc
     2161 gtcgccaagc agccggtagg caaggtgttt tgggaggcga tcccgtttat tatcgccctg
     2221 ctcattacgc tggccatcgt cacctacgtt ccagccatcc cgatgttcct tccaagggtg
     2281 cttcaggggc cgtagagaac ggttcaggca agtcgatgcg gggaggatga accggatgcc
     2341 cgggaagctt gaagtccgaa tcgaatactg cgcggaatga gggtatgagg gtcacgccgc
     2401 ccgtgtggcg gaggaattgc tggaagcgca cgcggagaac atcgcgagtc tcacgatcat
     2461 tccctcgtcc ggcggccgat ttcttgtgaa ggccggagcg gagcagttgt tcaataaaaa
     2521 ggaagctgga cggttcccca acaaaggcga aaccgcccaa ctcctggctc gaacctttag
     2581 cccgtaagat tcttactcat gcgcacggca acgccttggc caccacggtc aggctggaca
     2641 atggtgacct cgtccatcaa ttgtccgatc aagaagagcc cacgaccgtg tccgctctcc
     2701 ggcggagtct cggggatcgt gatccgatcc gtgacgggtt tgccgccgtc atggacgctc
     2761 accagaaagt ctttgccgtt gaaccccaag tccacgtcga tctggccctc ggtcttcccc
     2821 ccatacgcgt gcaggtaggc attcgtgacg gcttcgccaa ccgcaacctc gagatcgtag
     2881 gcatctgcct ctgaggcccc tgccaaccgg gcggctgaat tgacgagctt tcgcacggta
     2941 cgcatggccg tgggctcgct gtagacacgg atggtcagcg tccaggccat ggtacccgtg
     3001 ggcccgttat gcattagggg gaatatcgtc aagcgaactg cacctgcatt caattttccc
     3061 agaatcgcgc cttcaacacg ctgaaacagg aacgaacacg cacgctgcga aagcaagtct
     3121 gaaatctatt cgcgaggggt cacatgcccg gtcgccattt cctgcagatc cctggtccca
     3181 cgaacgtccc agatcgcatc ctcggagcga tgcaccggcc cgttcctgac caccgcggac
     3241 cggaactccc cgcgcttttg agcgagatcg tcgccggcct caagaagatc tttcagacga
     3301 cccgcggcga gatcgtgttg tttcccgtgt cggggacggg cggcctcgaa gcatcaatcg
     3361 tcaatacgtt gagtcaaggc gatctcgtgc ttgcgttcaa catcgggcat ttcagccact
     3421 cgtatgccga gacggcccgc ggttttggga tgcaggtcga tgaagtcgag cttccctggg
     3481 ggcagggtgt cccggcggat ctcgtccatg accgattgat gcgagatacc gcgcgcaact
     3541 acaaggcgat cctgatggtg cacaacgaga catcgacggg tgtggcctct gacgtgcgcg
     3601 ctgtccgaga ggccatcaat tcggctcatc accccgcgct gctgctggtc gacaccgtca
     3661 gctcactcgc cagcatcgag tttctgttcg atgagtgggg cgtcgacgtc gccgtgtgcg
     3721 gaacgcaaaa gggtctgatg ctgccgccgg ggatggcggt cgtgtgcgcc ggtccgcgcg
     3781 cgattgccgc gagcgcgcag gtaactactc cgcgcttctt ctttgactgg cgccccgtgc
     3841 tcgccgagat gaagcgtggc tactttccat ttacacccgc cacgctgatg ctctatggat
     3901 tgcgggaggc ggtccgaatg ctgctcgagg aagggctgcc ggctgtgttt gggcggcacc
     3961 gcaatcttgc cgagggcgtg cgccgcgcgg tgcgggcctg gaacctgccg atcctctgcc
     4021 gtaactctgc ggagtactca aactcgctga cagcggttga gcttcccgat gggattgatg
     4081 ccgataaggt gctgcagata gcccaggagg aattgaacct ttctcttggc ggagggctcg
     4141 gtcgcctcaa aggtcgagtc attcggatcg ggcatctcgg cgcgctcaat gagctggagg
     4201 tgctcgccac agtcgccggt accgagatgg ccctgcagat ggctggcatt cgaatcgatc
     4261 ccggggtcgg cgtgcgggcc tgccagcagt ggttcatgca ggggtaccgc gcaggaaccc
     4321 gtgtcggttc gcggcggtaa ttccccgtta ggtttcatgg aagtttgggt aaaactccgg
     4381 agaggattat agttgactca agtacatgct tgactcggcg tccgatatac gctaagatat
     4441 gatcgaactt aggaggtgaa tccacgatga ttcacgacac gaacgttaag gctgacacgt
     4501 tcaagcaacg gtggtccagc ctagtgccaa agatccggga tcggtacccg aacctgagcg
     4561 atcaggatct gtccgacatt aatggtgacc tggacaagct ggtcgccaag gtcagtgaga
     4621 agcacggcca gagcaaggcc gagattatga atgatctgac gaccttgatc agtgggcctg
     4681 agaagacggt agccggaaat accggaagca agatggggtc aggaagctct gaccagtatc
     4741 aacagaagga aggcttccca aagcagggcg tggagcagaa gcaaggacag aagccggact
     4801 tcggatcgga agggtctgtg aaggaaggct cagagagaga agagtctaca ggcaagaagc
     4861 ggtaagtgaa cgaattagcg gggggcggga aaccgcctcc cgctttttta atttccgaag
     4921 cactctatca ttgatggccc gctacgcacg tctcgcgtct ggactgtgga tcctgatgat
     4981 tctctttgcc tctctgctgc ccggaggcac cacgagggcg gcaggcacgg gttggcacat
     5041 cattggttat ggagtactcg tcgtgctgct ggccgcctgg caaccgatcg ttcgagccgc
     5101 ggtcgcggcg tggtcattag gagccgttgt tgaggggctg caactgctgg tggcatatcg
     5161 ggttgcagag tccggttacc ttgttgccaa cgccgcgggc gtcgcgctgg gattggcgct
     5221 gcgggccggt tacttcgctt ttgccagatg agctttaatc tgtttgatgc ctgatgggat
     5281 gacaatgctc ataaggtgat caggaaaact gcgggcgatc tccgctgtga tcagagtctc
     5341 ctcccagccg gccgataacc ccctggacac aatgtcctca accgcatcgt gatagcgact
     5401 ctgagcgtcg agcgcgcctg gtccgccggg tgccccgtgc ccgggaataa atgtctcggc
     5461 atccagtgac gccagggcct gcaacccctt gcgcgcaccc gcgatggatg cctgctcgta
     5521 gttgtaatgg tacccgctga acacgaggtc gccggaaatc gcgacaccta aggcggggat
     5581 aaacaggaac gcgtcacccg gcgtgtggcc ccaaccgggg tgccacactt cgatctcgat
     5641 tccgccgacg tggaggacca gcccctcgtc aaacgtgaca gacggcagga cggattcggc
     5701 gtcggcgaac agctcccgca gctcagggcg cgcgcgccgc tgttgaatga tgcccgggtg
     5761 ttcctccgcc atggcctcac ggcaggcgcg gtgagagaac acgacggcat cgcgtgccgc
     5821 gaaataggaa gagccgaggg cgtggtcggt gtgatggtgt gtcagcacga cgaaccgcac
     5881 gggcgcgcgg gtgaggtctt tcagcgcttc gtcgacgagc cgcgcgtggg ccggggcgat
     5941 cagcgggtcg acgagcaaga ccgcatcagt tccaatgacc ccgatgcagt tcgccccgta
     6001 gctggtctcg atcgtgtcgc cgccgatcgt ccagacgaag aggttgtcct ggattcgcgt
     6061 ttcatgcagg gcgggcatgc cccggtattc tctgattgcg aagacctctc ccgcccagca
     6121 cattgaggac gacgatgcct gcgacgatga gagcggtgcc tgcgtacagc cgccaatctg
     6181 gccgctcgtt caggaagacg atgccgagca gcaggccgat gattggaaag acatacgtca
     6241 cgacggatgc gcgcgtcggc ccccacgagt tgatgaggta gaaaaagagc agatacgcga
     6301 cacaggatcc cagcaacccg agccagagaa tcgccaccca cgtgatcggc agggccggcg
     6361 tctggatcgg ccgctcgagt ccaagcacgg ccagccaggc gaacgcgtcc ccggtcaaca
     6421 gcaccatcgt cccctgcaga acgggcggct ggtttcgcaa ataccgtcgt gagaatgtag
     6481 cggccattcc atagctggcg gcggccgcca gcactgccag ctgtccgagc aggctgccgc
     6541 tcagagcgcc cggccggaag tcgtcgctga ccaacaccac gacaccagcg aaacccacga
     6601 tgagaccaac cacgcgcatc gccgtcatct tctcgtcggt cagccagtag tgtgagatca
     6661 cgatcgcaaa caacgggacc gtaccgttta gaatcgcggc caggcctgag tcgatccgcg
     6721 tttctcccca cgagatcagc gagatgggaa gcatcgcctg gaacaaaccc atgaggaggt
     6781 aggcaggcag ctgcgcgcgt cctagaggta atcgctttct ctgcagacgc aggactccaa
     6841 gcaggccgat aaggccgaac agcaggcggt aggcgacgag agtgagaggg ccgatctctc
     6901 cgacac
//
LOCUS       DBNGSB010000002         5141 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3069414, whole genome shotgun sequence.
ACCESSION   DBNGSB010000002 DBNGSB010000000
VERSION     DBNGSB010000002.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 5141)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5141)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5141
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3069414"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="AC159B_00055"
     CDS             <1..888
                     /locus_tag="AC159B_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family lipoprotein"
                     /protein_id="HIR3376178.1"
                     /translation="ARAQKQFGMRIETRDLEPTAGGENREELLRLLAGEKFDLIFGIG
                     FLFTDSVTRVSKDFRNVKFGIIDGFIPDQPNVASLLFKEHEGSFLVGAAAGLKTKTNR
                     VGFVGGMKIPLIERFEAGFIAGATHVNPRVQVFSEYAGTTGEAFRDPVKGKELALAEY
                     DRGADIIYHASGGTGIGVFEAAVVKKRMAIGVDADQSLTVKPDQRRQILTSMMKRVDV
                     AVFQTISGMINGKYKAGVVTFGLKENGVGYAVNEFNRSRIRDIVPRLEALKRDIIAGK
                     IKVPEDKKQLAAFVKKVKR"
     gene            961..2529
                     /locus_tag="AC159B_00060"
     CDS             961..2529
                     /locus_tag="AC159B_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013920931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3376179.1"
                     /translation="MIVLRGVTKRFPGVVANDHIDLEVRRGEIHALVGENGAGKSTLM
                     RILYGLYQPDEGTIEVNGRPVVIDSPRTALTLGIGMVHQHFMLIPRFTVAENVILGSE
                     PTGAFGRLQRREAEERVRELCQQYGFALDPAAEVSMLSVGEQQRVEIIKVLYRGADVL
                     ILDEPTAVLVPQEVDELFMNLRKLREQGKTIIFISHMLDEVLEISDRITVLRRGRVVG
                     TVPAAGTTKSQLAEMMVGRPVLLELEHPPVTPGDVRLEAQEITVAGRGGKPAVHRLSL
                     AVRAGEIYGIAGVEGNGQTEFVEALVGLRQVISGRLLIDGREVTHATARAVRLLGVAN
                     IPEDRHRRGLVLPMAVRENVVLGHHGQQQFLNGAFIDRRAVETFADEQVEKFDIRLSG
                     TDVPALALSGGNQQKVIVAREFSFSPKVLIAAQPTRGLDIGATEFVERQLLEARSGGM
                     AVLLISANLEEVLSLSDRIGVMFAGELVAEFRRGEATPQELGLYMTGTRRALNPRGAS
                     GSGGQTAGTGVSPS"
     gene            2526..3659
                     /locus_tag="AC159B_00065"
     CDS             2526..3659
                     /locus_tag="AC159B_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3376180.1"
                     /translation="MKWRLLALQIATPLLAILFGVLIASIIVLAIGQNPLDVFGVLIR
                     FGLLRSDSLLSILFKATPLIFAGLAVALSFRAALWNIGVEGQFYIGAFTAAYVGFTLK
                     GLPVAIHLPLAILAAAAGGMVWALIPIVLKLRRGAHEVITTIMMNYIAAALILYLISD
                     VFRDPSQVGTPRVRTPEFPGSARVPSMGPLLGLFGLTGPDANKVNWLLPFGMVLCVVF
                     AIVLSRTRFGYEVRAVAFNPYAAEAAGIPNARIQFLMFMLSGAVAGLVGLNDVLGFFG
                     YLDIDFPKGLGFLGISVALLAKNNPLGVVLAALLFGFLDRGAQGVQFFARTPKEVIVI
                     LQALIILAIVVAYELMTRYIRKQRKREAERPPPTVTVAAEAGR"
     gene            3660..4631
                     /locus_tag="AC159B_00070"
     CDS             3660..4631
                     /locus_tag="AC159B_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3376181.1"
                     /translation="METLVSLLTLALIASAIRVTMPILLAALGAVYSERGGVINIGLE
                     GIMIMGTFFGAVGDYVFAQRFPGLVESVPDLAPAAGVLVAILAGVLFACIHALVTVTF
                     RVDQIISGVALNLLAVGAARFLNILFFEVSTQSPGVSGFTPINIPILKDIPVLEPIAT
                     NISPMIPAALLLVLVSHWVLLRTRFGLRLRAVGEHPLAADTLGINVIRTRYAGVMISG
                     ALAGMAGAYLAIEQAHLYFEGMTQGRGFIALAAMIFGNWWPLGALGASALFGYFDALS
                     LRVVRLPVPSQFINVLPHIVSILVLAGFVRRAQPPAADGIPYSKEEE"
     gene            4721..>5141
                     /locus_tag="AC159B_00075"
     CDS             4721..>5141
                     /locus_tag="AC159B_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR3376182.1"
                     /translation="MTDDRRPHVVVVGGSNIDLIGIAPSPMLPRISNPGRVRIGPGGG
                     GRNVAENLARLGARVTLISTVAAGPFGDWLIEQTAAAGVDVAKAERVQGQGSYYIGIQ
                     SDGVLQWGVSDMAASESLTPAAMDARAELIRAAEAVVV"
BASE COUNT          886 a         1556 c         1672 g         1027 t
ORIGIN      
        1 gcgcgcgcgc agaagcagtt cggcatgcgg attgaaaccc gcgatctcga gcccacggcc
       61 ggcggcgaga accgagagga actgctgcga ctcctggctg gagagaagtt cgatctcatt
      121 ttcgggattg gattcctctt cacggacagc gtcacgcgtg tctccaaaga ttttcgcaac
      181 gtgaagttcg gaattattga cggcttcatc ccggaccagc cgaacgtcgc gagcctgctg
      241 ttcaaagagc acgaaggctc gttcctggtc ggcgcggccg cggggttgaa gacgaagacc
      301 aacagggtcg gatttgtggg tggcatgaag atcccattga tcgaacggtt cgaagccgga
      361 ttcatcgccg gtgcgacaca cgtgaacccg cgagtgcagg tcttctctga gtacgccggc
      421 acgaccggtg aagcgttccg cgatccggtt aagggcaaag agttggccct ggccgaatac
      481 gatcgtggtg cggatatcat ctaccacgcg tccggcggaa ccgggatcgg cgtcttcgag
      541 gcggcggtgg tcaagaagcg gatggccatc ggggtggatg ccgatcagtc gctgacagtg
      601 aagcccgatc agcgccggca gatcctgacg agcatgatga agcgcgtcga cgtcgccgtc
      661 ttccagacta tcagtgggat gatcaacggc aagtacaagg ccggtgttgt gacgttcggt
      721 ctcaaggaga acggagtcgg ctacgccgtc aatgagttca accgcagcag aatccgagac
      781 atcgtgccca gacttgaagc cttgaagaga gatatcatcg ccgggaagat caaggtgcct
      841 gaggataaga agcaactggc ggcatttgtg aagaaggtca agcgatagtg tcacactagg
      901 ccatccatcg cggggtgggg ccagccctca ccccgcgatt cttttcgcct gagaccgaca
      961 gtgattgtcc tacgcggcgt cacgaagcga tttcccggcg tggtcgccaa cgaccacatc
     1021 gatctcgagg tccgccgggg cgaaatccac gctctcgtcg gcgagaacgg tgccggcaag
     1081 tccacgctca tgcggattct gtacggcctc taccagccgg atgagggcac gatcgaggtc
     1141 aacggccggc cggtggtcat cgacagtccc cgcaccgcac tgaccctggg catcggcatg
     1201 gtccaccagc acttcatgct catcccccgg ttcacggtcg cggagaacgt catcctgggc
     1261 agcgagccga cgggggcatt cggacgtctg cagcggcgcg aagcggaaga acgcgtccgc
     1321 gaactctgcc agcagtatgg gttcgccctg gatcctgcgg cagaggtcag tatgctgtcc
     1381 gtcggtgagc agcagcgtgt ggagatcatc aaggtgctct accgtggcgc cgacgtgctc
     1441 atcctggacg aaccgaccgc ggtcctcgtc cctcaagaag tggacgagct attcatgaac
     1501 ctgcgcaagt tgcgcgagca gggaaagacg atcattttca tcagccacat gctggacgaa
     1561 gtgctcgaga tctcggatcg catcaccgtc ctgcggcgag ggcgggtcgt cggcacggtg
     1621 cccgctgcgg ggactacgaa atcgcagctc gcagagatga tggtcgggcg gcccgtgctg
     1681 ctcgaactgg agcatccgcc cgtgacgccc ggcgacgtcc gccttgaagc gcaggaaatt
     1741 acggttgccg ggcgcggcgg aaagcctgcc gttcaccgtt tgtcactcgc ggtacgcgcg
     1801 ggtgagatct acggcattgc cggtgtcgag ggcaacggtc agaccgagtt cgtggaagcg
     1861 ctggtcgggc tgcgtcaggt gatctctgga cggctcctca tcgatggtcg cgaggtgacc
     1921 cacgccactg cgcgggccgt gcgcctcttg ggtgtggcca atattcccga ggatcgccat
     1981 cgccgtgggc tggtattgcc gatggcggtc cgggagaacg tcgtattggg ccaccacggc
     2041 cagcagcagt tcctcaatgg cgcgtttatc gaccggcggg ccgtcgagac atttgccgac
     2101 gagcaggtag aaaaattcga catccggctg agcggtactg atgtcccggc cctcgcgctt
     2161 tcggggggca accaacagaa ggtcattgtc gcccgggagt ttagtttcag tccgaaagtg
     2221 ctcatcgctg cgcagccgac ccgcgggctc gacatcggcg cgacagagtt cgtggagcgc
     2281 cagctgttgg aggcgcggtc gggcgggatg gcggtgctgc tcatttccgc caaccttgaa
     2341 gaggtcttgt ccttgtccga ccggataggc gtgatgttcg ccggagagct cgtcgccgag
     2401 ttccggcgcg gcgaggccac gccgcaggaa ctggggctct atatgacagg cacccgccgt
     2461 gccctgaacc cgaggggagc gagtggttca gggggccaga ccgccggcac cggggtttcg
     2521 ccctcgtgaa gtggcgcctt cttgccctgc agatcgcgac gccgctgctc gcgatcctct
     2581 tcggcgtgct tatcgcctcc atcatcgtgc tggccatcgg ccaaaatccg ctcgacgtct
     2641 ttggcgtcct gatacgcttc ggactgcttc gatctgacag tctgctctcg atcctcttca
     2701 aggccactcc tctgatcttc gccggcctcg ccgtcgcgct ctcattccgc gcggcgctgt
     2761 ggaacatcgg cgtggaagga caattctaca tcggcgcgtt taccgcggcc tacgttggat
     2821 ttacattgaa gggcctgccg gtcgccatcc accttccgct ggcgatcctg gccgcggcag
     2881 cggggggtat ggtgtgggcg ctgattccaa tcgtgctgaa gctccggcgc ggcgcgcacg
     2941 aagtcatcac aacgatcatg atgaactaca ttgccgctgc cctcatcctc tacctgattt
     3001 ccgacgtctt ccgtgatcca tcccaggtcg ggaccccgcg ggtccggacc ccggagttcc
     3061 ctgggagcgc gcgcgtgccg tccatgggac cactgctggg cctgttcgga ctgaccggtc
     3121 cagatgcgaa taaagtcaat tggctcctgc cgtttggtat ggtcctgtgc gttgtcttcg
     3181 ctatcgtgtt gagccgcacg cgtttcggtt atgaggtccg cgccgtcgcg ttcaacccct
     3241 atgcggcaga agcggcgggc attcccaatg cacggatcca gttcctcatg ttcatgctga
     3301 gcggcgcggt cgcggggctg gtcggcctca acgatgtgct cgggttcttc ggatacctgg
     3361 acatcgactt cccgaaggga ctcggctttc tgggtatttc ggtcgcgctg ctggcgaaga
     3421 acaatccgct gggggtcgtt ctggccgcgc tgctgtttgg tttcttggac cgcggcgcgc
     3481 agggggtcca gttctttgcg cgcacgccca aggaagtcat cgtgattctc caggccctca
     3541 tcattctcgc gatcgtcgtg gcctacgagc tgatgacacg ctacatccgg aagcagcgga
     3601 agcgcgaagc cgaacggcct ccccccacag ttacggttgc cgcagaggcc gggcgctaga
     3661 tggaaaccct ggtgtcgctg ctgaccctgg cgttgatcgc gtcggcgatc cgggtgacga
     3721 tgccgatcct gctggctgct ctcggcgccg tctactcgga gcgcggcggc gtcatcaaca
     3781 tcgggctcga aggcatcatg atcatgggca cattcttcgg tgccgttgga gattacgtgt
     3841 ttgcccaacg ctttcccggt cttgtggaat ctgtaccgga cctggcgccg gcggccggag
     3901 tcctggtcgc gattttggcc ggagtcctct tcgcttgtat tcacgcgctg gtgacggtca
     3961 cgttccgtgt ggatcagatc atcagcggcg tcgccctcaa ccttctggcc gttggggctg
     4021 cccggttctt gaatatcctc ttctttgaag tctcgacgca gtcgcccggc gtcagcggct
     4081 ttaccccgat caatatcccg atcctgaagg acatccccgt gctggagccg attgccacaa
     4141 acatctcgcc gatgataccg gccgcgctgt tgctcgtcct ggtcagccac tgggtcttgt
     4201 tgcgcacaag gtttgggctg cggctccggg cagtgggaga gcatccgctg gcggccgaca
     4261 ccctggggat caacgtcatc aggacgcgct acgcaggcgt gatgatcagt ggagccctcg
     4321 ccggcatggc cggggcctat ctggccattg agcaggcgca cctctacttc gaggggatga
     4381 cgcagggacg cggcttcatt gcgctggccg cgatgatctt cggaaactgg tggccgctcg
     4441 gggcccttgg ggcgtccgca ctcttcgggt acttcgacgc gctcagcctg cgcgtcgtgc
     4501 ggctgcccgt gccctcccag ttcatcaacg tcctcccgca catcgtgagc atcctcgtcc
     4561 tcgcagggtt cgtacgccgt gcgcagcccc cggcggcgga tggcatccca tacagtaagg
     4621 aagaagaata acggtcatag gtcgtgggtc gtaggtcgtc gctaatgaca tcgtgggcgc
     4681 ccaaactgtt ggctgagatc tgtcaggttt tctacgacct atgaccgacg accgacgacc
     4741 gcatgtcgtg gtggtgggcg gctcgaacat cgatctcatc gggatcgctc cgagcccgat
     4801 gctgccgcgg atcagcaatc cgggtcgtgt gcgcatcggt ccaggcgggg gagggcggaa
     4861 cgtcgctgaa aacctggccc gtctgggcgc gcgcgtcaca cttatcagta cggtggccgc
     4921 gggcccgttc ggagattggc tgatcgaaca gaccgcagcg gccggggtcg acgtcgcgaa
     4981 ggctgagaga gttcaggggc agggaagcta ttacatcggg atccaatcag acggcgtcct
     5041 ccaatggggc gtctcggata tggccgcgtc ggagtcgctc accccggcag cgatggatgc
     5101 gcgcgcagaa ctcatccgtg ctgctgaggc cgtcgtcgtg g
//
LOCUS       DBNGSB010000003         5697 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3683187, whole genome shotgun sequence.
ACCESSION   DBNGSB010000003 DBNGSB010000000
VERSION     DBNGSB010000003.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 5697)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5697)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5697
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3683187"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..331
                     /gene="ssrS"
                     /locus_tag="AC159B_00080"
     ncRNA           153..331
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="AC159B_00080"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(422..709)
                     /locus_tag="AC159B_00085"
     CDS             complement(422..709)
                     /locus_tag="AC159B_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR3376183.1"
                     /translation="MAKAMTKSKIADHLAGKLDVPKKTANTFLDELATLSYREAKNTF
                     TIPGVGKLVLRNRKARMGRNPQTGEPIRIPAKRVVRFRVSKAAKDAILGGR"
     gene            865..2232
                     /locus_tag="AC159B_00090"
     CDS             865..2232
                     /locus_tag="AC159B_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HIR3376184.1"
                     /translation="MDQRKRKDVELFKQGTAERLAAEAPLAARMRPRTLDEFVGQEHL
                     LAPGKVLRRAIESGELTSLILYGPPGTGKTSLAHVIARHTDSSVEQINAFTAGVAELR
                     EVIARAKDRRMLHSRRTILFVDEIHRFNKAQQDVLLPYVEDGTVVLIGATTENPFFEV
                     TATLVSRSRVFRLEPLGEDALRTILTRAVSEPERGLGAFRVEVDEEAMTHLIGVSNGD
                     ARIALNAVELAAMTAPPDTAGVRRVTLQSIEEAAQRRALVYDRAGDAHYDHISAFIKS
                     MRGSDPDAAVYWMARMIAAGEDPRYIARRMVIHASEDVGMADPMALVVATAAAHAVEF
                     VGLPEAQIPMAQAAIYIATAPKSNAVVRAIGSAMSDVEERRADQVPLHLRETGHPLVA
                     KHLGHGRGYKYAHDYAGGFVPQQYLPDSVKDRVYYHPSDAGFEQEISKRLEGWWKGLK
                     RYIPE"
     gene            2303..3229
                     /locus_tag="AC159B_00095"
     CDS             2303..3229
                     /locus_tag="AC159B_00095"
                     /inference="COORDINATES: protein motif:HMM:NF013567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3376185.1"
                     /translation="MRVGMIGTGDFARRHLQVLSRESEVEIVGHVSALRERAEAQAKD
                     WGGHAYGSAQDLVRGASPDAVWISVAPSAHGAIESALIDGGVPFFVEKPVAVDLATAE
                     SIAVKLERRRVIAAVGYHWRAMDTIPEVRSVISSRSVRLVEGTWLDTLPPCRWWHKRD
                     TGGGQIIEQATHPIDLARSLVGGAEVLGMTESGARSDEIDVAMATAALLRFGNGAPGV
                     FTAANVLEGPAAVYLRLVCDGMLITIQRNQVTYETGRRARDVLVRNDPIVDEGRAFLQ
                     AVRSRDQSKVFCTYPEALESHRLCCAIAAAAR"
     gene            3334..4638
                     /locus_tag="AC159B_00100"
     CDS             3334..4638
                     /locus_tag="AC159B_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014510315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3376186.1"
                     /translation="MRLWRILALLGVLVLAASAPLGSAPSVEVTWSSTQLNPVNEQEW
                     ARNTLLKPFEDESSMRVRFVSDLAGPFIDRLVAEAKAGRGTVDIVGTLHGDFPLLVAE
                     GAVQDVGAVLRQLDGRPDRTFVPDLVRAGQIGGIQSFVPWMQATYLIAAHKQAMRYFP
                     AGRDPMRMTYDDLLQWGENIRSATGQRRIGFPALPSGLYGRFLHGYAYPSFTGSQVKK
                     FKSPEAVAMWRYLRRLWGVSHPSSFTYGFMNEPLLRGEVWVAWDHVARLLPAFRERPD
                     DFVALPSPAGPRGRSFLAVIVGLGIPKTSPNPEAAARLIEYLTRPRTQVMTLQGVGFF
                     PVVREAAGVVPTGGLKVMAEGVTAQATASDAHTALLPIGLGARTGEFVPLYLETFTLI
                     AIQGRNIEQVLNDQARKLEELYRATNAPCPPPDPAERPCRPD"
     gene            4698..5603
                     /locus_tag="AC159B_00105"
     CDS             4698..5603
                     /locus_tag="AC159B_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015231636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3376187.1"
                     /translation="MTVAEQRLEHALPPVRRQAVDWLPYVLLLPTVVFLTVFFVYPML
                     QAFVLSFRAPDGALTVRYFQAMLRDTYFAGALRNSFLLTAMIIPLQVFLALVIALLIN
                     TRFRGHPWFLFICAIPLGISDLGAGLIWSSFFTERGYLNLILHNLGLIKEPITFLTFE
                     RPAWLYGAIVATEVWRATAIVMVILVAGLQLIPKDYFETADVFGASGWRKLRHVTLPL
                     LRPALQTALIVRTILAFQLFATVIVMAGRIVPVLAGEAYFQYGLMRNVHVASAYAVLI
                     MVLSMTITIFYLWALRSKEEEQGRG"
BASE COUNT          993 a         1747 c         1833 g         1124 t
ORIGIN      
        1 gtgctcctga atcaaaccaa tatccgtgaa gtgaccttcc tcttcagggg gccgacccga
       61 cttcacccct aagaaccggg aacggggaac agggaacggt caaagattta cggtttcccg
      121 ttccccgata gccgttcccg ttgtactata ggagtgtcct gccgtgcgcg tggggccgct
      181 agttccgatc caacacgttg cacaacggga gcccggctct gttcgtcgat gacacgcctc
      241 gatgtaccga ggtagtcaga agcgcgcagg agggcaccca cctgggagac caggttcggg
      301 aactgcagtc ctacgacacg gcggggaaat gacgcgggcc tcgacgtctg tcgaggcccg
      361 cgcagtcata tgagtccctg cttctaacgg cgccgccgtg aatccgagcg acgcgagtgg
      421 ttcatcggcc gccgagaatg gcgtccttgg ccgccttact cacacgaaag cgcacgacgc
      481 gcttggccgg aatccggatc ggttcgccgg tctggggatt gcgccccatc cgcgccttgc
      541 ggttccgcag cacgagtttc ccgaccccgg gaatcgtgaa cgtgttcttc gcctctctgt
      601 atgaaagagt ggccagctcg tcaagaaacg tgttcgcggt cttcttcgga acatcgagct
      661 tgccggccag gtgatcagcg atcttcgact tcgtcatggc ctttgccatc tacccacccc
      721 cgtctaggag tcatgtccgg atgcgatttc ctgcgtcaaa cctgcatttc ctgcatgagt
      781 ataggaatgt gctccaggac tgtcaagcaa tactgatgcg cctgacgcgc caaaaatcgg
      841 tatgttacga tcagagcagc tttcatggac cagcgaaaga ggaaagacgt ggagctgttc
      901 aagcagggga cggctgagcg gctggcggcc gaggcgccgc tggccgctcg gatgcggccg
      961 cgcacgctcg atgagtttgt cggccaggag cacttgctgg cgccgggcaa ggttcttcgc
     1021 cgggcgatcg aaagcggtga actcacctcg ctgatccttt atggcccgcc gggaacggga
     1081 aagacgtccc tcgcgcatgt gattgcgcgg cacaccgatt cctcagtcga acagatcaac
     1141 gccttcaccg cgggcgtggc cgaactgcgc gaggtgatcg cgcgcgcgaa ggaccggcgc
     1201 atgctgcaca gccggcgcac catcctcttc gtcgatgaga tccatcgctt caacaaggcc
     1261 cagcaggacg tgctgctccc ctatgtggag gacgggacgg ttgtgctcat cggcgccacg
     1321 accgagaatc cgttcttcga ggtgaccgcc acgctcgtgt cacggtcccg ggtgtttcgg
     1381 ttggaaccac tcggcgagga cgcgctgcgt acgattctca cgcgtgccgt aagcgaacct
     1441 gagcgtggtc tcggcgcgtt ccgcgtcgag gtggacgaag aggccatgac ccatctcatc
     1501 ggcgtcagca acggcgacgc gcgcatcgca ctgaacgcgg tcgaactcgc ggcaatgacc
     1561 gcaccgcccg ataccgcggg agtccgcagg gtcacccttc agagtatcga ggaggccgcc
     1621 cagcgccgtg cgctcgtgta tgatcgcgcg ggcgacgcgc attacgatca catttcagcc
     1681 ttcatcaaga gcatgcgcgg ctcggatccg gatgcggcag tgtactggat ggcgcgcatg
     1741 attgccgcgg gagaggatcc acggtacatt gcgcgccgga tggtcattca cgcgtcggag
     1801 gacgtgggga tggcggatcc catggcactg gttgtggcga cggccgcggc acatgccgtg
     1861 gaattcgttg gcctccccga ggcgcaaatc ccgatggccc aggccgccat ctacatcgcg
     1921 accgcgccca agagcaacgc ggtcgttcgc gccatcggtt ccgcgatgtc cgacgttgaa
     1981 gagcgaagag cggatcaggt ccctttacac ctgcgagaga ccgggcatcc gttggtcgcc
     2041 aaacacctgg ggcacggacg cggatacaaa tatgcccacg actatgcggg cgggttcgtc
     2101 ccgcagcagt atcttccgga cagcgtgaaa gaccgcgtct actaccaccc gtccgatgcc
     2161 gggtttgagc aggaaatcag caagcgcttg gaaggttggt ggaaaggcct caaacgctat
     2221 atcccagaat gacactctcc aaagcgttca cgctgctcga tgcccaaagc caccacgcgc
     2281 tgcaggtcgt cctcgaattc acatgagggt cggaatgatc ggcaccggtg attttgcgcg
     2341 ccggcacctt caggtgctct cgcgagaatc tgaagtcgag attgttggcc acgtgtctgc
     2401 gcttcgagag cgcgccgaag cccaagcaaa ggactggggc ggacacgcgt acggcagcgc
     2461 tcaggatctg gtgcgggggg cgtcgcccga cgccgtgtgg atctccgtcg cgccctctgc
     2521 ccacggcgcg atcgaatcgg cgttgatcga tggtggagtg ccgttcttcg tcgagaaacc
     2581 ggtggcggtc gatctggcga cggctgaatc catcgcggtg aaacttgaac gcaggcgggt
     2641 catcgcggcg gtgggatatc attggcgcgc gatggatacg attccggaag tccgctccgt
     2701 gatttccagc cgctcggtgc gcttggtgga agggacctgg ctggatacgt tgcccccttg
     2761 caggtggtgg cacaaacggg acaccggcgg cgggcagatc atcgagcagg ccacgcatcc
     2821 catcgacctc gcgcgatccc tcgtcggcgg ggccgaggtt ctcgggatga ccgagagcgg
     2881 cgcgcgcagc gatgagatcg acgtggccat ggccaccgcc gcgctgctgc ggtttggcaa
     2941 tggggcgcca ggagtcttca ccgcggccaa tgtgctcgaa ggtcccgcgg ccgtctacct
     3001 gcgcctcgtc tgcgacggca tgttgattac catccagcgg aaccaggtga cgtatgagac
     3061 cggccggagg gcgcgcgatg ttctggtgcg caatgatccc atcgttgatg agggtcgcgc
     3121 atttcttcag gccgtccgtt cgcgcgatca gtccaaagtc ttttgtacat atccggaagc
     3181 gctcgagtca caccggttgt gctgtgcgat cgcagcggca gcccgctagt ccgggaggag
     3241 gggggccgca gcgagcgcga acactgacaa cacccatcag gattctgctc tgtacctttc
     3301 gcacgtcagt cttgctttga gggagggacc aacgtgaggc tgtggaggat tctcgcactg
     3361 ctcggagtgc tcgtgcttgc cgcgagcgcg ccgctaggat ccgcgccgtc ggttgaagtc
     3421 acctggagct cgacccagct gaacccggtc aacgagcagg agtgggcccg caacaccctg
     3481 ctgaagccgt tcgaggatga gtcaagcatg cgggttcgct tcgtcagtga tttggccggt
     3541 ccgttcattg accgcctggt agcggaggcc aaggccgggc gcggcacggt cgatatcgtc
     3601 gggacgctgc atggcgactt cccgctcctc gtcgcagagg gcgccgttca ggatgtaggg
     3661 gcggtccttc gccagctcga cggccggccc gaccggactt tcgtgccgga ccttgtccga
     3721 gcgggtcaga taggtgggat ccagtctttt gtcccatgga tgcaggccac ctatctcatc
     3781 gctgctcaca agcaggcgat gaggtacttc ccggcgggac gcgatcccat gcgcatgacg
     3841 tatgacgacc tgctgcagtg gggcgagaat atcaggagcg ccaccgggca gcgccggatc
     3901 ggatttcctg cgctcccatc gggattgtac ggccggtttc tgcacgggta tgcgtatcca
     3961 tcgtttaccg gctcacaggt caagaagttc aagtcgcccg aggcagtcgc gatgtggcgg
     4021 tatctccgcc gcctgtgggg ggtcagccac ccatcgtcgt tcacatacgg cttcatgaac
     4081 gagcctctgc tgcggggcga ggtctgggtc gcgtgggatc acgtggcccg tctattaccc
     4141 gcgttccgcg agcgccccga cgactttgtc gctctccctt ctccagccgg gccgagagga
     4201 cgatcattcc tggcggtcat cgtcggtttg gggattccca agacctcccc gaaccctgag
     4261 gccgctgcgc ggctcatcga atacctgacg cgcccgcgca ctcaggtgat gaccttgcag
     4321 ggggtggggt tctttcctgt ggtgcgtgag gcggccgggg tcgttcccac cggcggtctc
     4381 aaggtcatgg cagaaggcgt aaccgcacag gcgacggcca gcgatgccca cacggcgctg
     4441 ctgccgatcg gactcggagc gcgcaccggc gagttcgtcc cactgtacct tgagacgttc
     4501 acgttgatcg cgatccaggg gaggaatatc gagcaggtgc tgaacgatca ggcacgcaag
     4561 ctcgaggagt tgtaccgcgc gaccaacgcc ccgtgcccgc cgcctgatcc ggcggaacgg
     4621 ccatgtaggc ccgattaggc tggggtccgg aatcggaccc taggccggac acgagcgtcc
     4681 atgagaggtc ggcagccatg actgttgccg aacagcgtct cgagcatgcg ctccctccgg
     4741 tccgccgtca ggcggttgac tggcttccgt acgtcctgct ccttcctacg gtcgtgtttc
     4801 tgacagtatt tttcgtgtat cccatgctgc aagcattcgt gctcagcttt cgggctccgg
     4861 acggggcgct cacagtgcgc tactttcagg caatgcttcg cgacacgtat tttgccggcg
     4921 cgctgcgtaa cagtttcctt ctcacggcga tgatcattcc gctccaagtg ttcctggcgc
     4981 tcgtgattgc gctgctgatc aacacgcgct tccgcggcca tccctggttt ttgttcatct
     5041 gtgccattcc gttgggcatc tctgatcttg gcgccgggct catctggtcg tcgttcttta
     5101 ctgagcgtgg atatctgaac ctgatcctcc acaacctggg gctgatcaaa gagccgatca
     5161 cgtttctcac gttcgagaga ccggcctggc tgtacggcgc gatcgtggcc accgaagtgt
     5221 ggcgggcgac ggcgatcgtc atggtgattc tcgtggcagg cttgcagttg atccccaaag
     5281 attactttga aacggccgac gtcttcggcg ccagcggctg gcggaagttg cggcacgtga
     5341 cgctgccgct gttgcggccg gcgctgcaga ccgcgctcat cgtccgcacg atcctggcgt
     5401 tccagttgtt cgcaaccgtc atcgtcatgg ccggccgcat cgtcccggtg ctggcgggag
     5461 aagcctactt ccagtacggg ctgatgcgca acgtgcatgt cgcgagcgcg tatgcggtgc
     5521 tcatcatggt gctctcgatg accatcacga tcttctacct ttgggcgctg cgcagcaagg
     5581 aagaggagca gggacgcgga tgacggggcc gggccggaag gcggtgctgg cggccctcgt
     5641 tgtcttgatc tccgcgtggg tgctgttccc tatcatcctg attgcaatgg ccagctt
//
LOCUS       DBNGSB010000004         7318 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2610190, whole genome shotgun sequence.
ACCESSION   DBNGSB010000004 DBNGSB010000000
VERSION     DBNGSB010000004.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 7318)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7318)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7318
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2610190"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="AC159B_00110"
     CDS             <1..180
                     /locus_tag="AC159B_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376188.1"
                     /translation="EYAKLWGLSFIRLVNHLVRVILDGQPVEPLATFRDGLQVQRTMA
                     AVRASAQRGWVSVAS"
     gene            complement(166..747)
                     /locus_tag="AC159B_00115"
     CDS             complement(166..747)
                     /locus_tag="AC159B_00115"
                     /EC_number="2.7.1.160"
                     /inference="COORDINATES: protein motif:HMM:NF014001.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000215 - tRNA 2'-phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0003950 - NAD+ poly-ADP-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006388 - tRNA splicing, via
                     endonucleolytic cleavage and ligation [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HIR3376189.1"
                     /translation="MEQQRPFRPASHGGNRSERLSRFLALVLRHKPESIGLELDPSGF
                     ADVDALVKAVADQPGWDWVTEQEVRGLVQHDPRRYEIDGTRMRARYGHSIPVETPGRS
                     VLPPEWLYHGTVGTALEAIRVGGLRPLGRQYVHLSATRQDAISVAKRHSDEVVLVTVL
                     ARRAHEAGFAFYQAAPSIYLVREVPPEFLSAGD"
     gene            773..1666
                     /gene="ftcD"
                     /locus_tag="AC159B_00120"
     CDS             773..1666
                     /gene="ftcD"
                     /locus_tag="AC159B_00120"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HIR3376190.1"
                     /translation="MPTLVECVPNFSEGRRQEVIDAIADEVRRTKGARLLDVQADESH
                     NRCVVTFVGPPEAAKAAALAAAGRAVELIDMNGHHGEHPRLGAIDVIPFIPISGVTMD
                     DAVATAQALGREIWERLRVPVFFYAEAATRPERRRLPDIRKGEYEGLAAKLTDPAWMP
                     DVGEAKPHPTAGATVVGARRPLIAYNINLSTDDIEVAKKIARAVRESSGGLAAVQAMG
                     VKSESGKAQVSMNLVDFTQTPVQRAFEVVKEEAAHYGVTILESEVVGLLPLEAIVDVA
                     RHHLRLRDFTREQILETHLME"
     gene            1713..3002
                     /gene="hutI"
                     /locus_tag="AC159B_00125"
     CDS             1713..3002
                     /gene="hutI"
                     /locus_tag="AC159B_00125"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HIR3376191.1"
                     /translation="MKQSVDLLITHAAQLLTIAGNNGHARSGDGLADLGLVTDGAVAV
                     ADGVIVAAGSTAQVLDEVRPSTGSTTIDARDAVVLPGFVDPHTHLVFAGSRADEFEMR
                     LAGATYQQIASRGGGILSTMRATRAATEDDLLALGSLRLDRMLLHGTTTVEAKSGYGL
                     SVEDELKSLRVVHRLSALHDADLVPTFLGAHAVPPEFAGDPDGYVRLVIEEMIPAVVE
                     EDLAEFCDVFCDVGAFNPDQSRSVLLAGHEAGLDLKIHADEMSDQGGARLASELEAFS
                     ADHLLRASEEGLRAMAESGTLGVLLPATALFLGLSYAPARRMIELQVPVAIGTDFNPG
                     TSPTYSMPMVIALSSLGMKMTPAEALVAATINAAHAVGMAEEVGSLEPGKAADLVILE
                     MDDYRQVAMTFGVNPVRTVIKRGRVVVDRGRLLRENQ"
     gene            2999..3619
                     /locus_tag="AC159B_00130"
     CDS             2999..3619
                     /locus_tag="AC159B_00130"
                     /inference="COORDINATES: protein motif:HMM:NF016825.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HIR3376192.1"
                     /translation="MISEPLTRFLDRLASAEPTPGGGSASAVAGAMAAALLAMVSRLT
                     KAKEGSASIEAVAGKMDQGRKTLLDLAAKDAQAFEDVLRAMRLAKETDDQRRERQQAI
                     QRALIEAAAVPLAVAAHAHDVLKSAVLVAREGNVNAVSDAGVAALLVHAAVHGAILNV
                     RINLSSIKDHGYVEATEARAKELGSASDRLRDEVMGIVHSRLARLG"
     gene            3619..3984
                     /gene="folB"
                     /locus_tag="AC159B_00135"
     CDS             3619..3984
                     /gene="folB"
                     /locus_tag="AC159B_00135"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HIR3376193.1"
                     /translation="MADRIVLAGMKFFAYHGVLPIERSTGQEFVVDVELEADLSEAAR
                     ADDLEHTVDYSRVYDIVWEIMQAEPRQLLETVADAIARQLLTLDRVQAATVRVRKPQV
                     RLPGPLDYSSVEIRRARRR"
     gene            3984..4466
                     /gene="folK"
                     /locus_tag="AC159B_00140"
     CDS             3984..4466
                     /gene="folK"
                     /locus_tag="AC159B_00140"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HIR3376194.1"
                     /translation="MARVYMSLGSNLGDRKATLESALRHLKASRHVSVLRASSIYETE
                     PIGYADQPWFYNLAAEVETELDPDELLALTKQVERDLNRTRQIRWGPRTIDIDILLYD
                     DIVVNGDRLTLPHPEMTQRRFVLEPLHEIAPELKLPDGRRIADLLPNVSAQEVRRVAV
                     "
     gene            4456..5223
                     /locus_tag="AC159B_00145"
     CDS             4456..5223
                     /locus_tag="AC159B_00145"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin--[biotin carboxyl-carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HIR3376195.1"
                     /translation="MQSSAIHDGLRTQRLGRPLKVFESVGSTNDAAREMAEAGAPEGV
                     AVLAGEQTGGRGRKGSSWASPAGGLWLSVVLRPDLPLEAWPLIGFAAGAGAAAALESV
                     AGQTVQLKWPNDLILDGQKVGGILVESAGGFAILGIGINVNFGKDALPEDVRRGATTV
                     LASLGREVELAVLTRAILEQVEYFYDVIHRDPKAVLRAWRMRSFLTGRSVQVSGTRVL
                     EGVAEDVDDSGALLIRTASGVQAVTVGDVSVRETRAI"
     gene            5227..6171
                     /locus_tag="AC159B_00150"
     CDS             5227..6171
                     /locus_tag="AC159B_00150"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HIR3376196.1"
                     /translation="MPQITDVPGIKVGHATDKRAATGCTVILCERGAVGGVDVRGAAP
                     GTRETDLLRPMNLVERVHAVLLTGGSAFGLAAADGVVRFLEERGVGFETGGTKVPVVP
                     AAVIFDLAVGSAKVRPGPEMGYAAAREAAEKVEEGSVGVGTGASVGKLLGPVSAMKGG
                     VGTWSLRLPGGVVIGALVVVNAFGDVIDTDNGAILAGARDPATGHFLNMTETLLSMEE
                     MPGFPGNSTLAVVATNARLTKEQANKLAQLAHDGLARVITPAHTMFDGDTVFALSTGE
                     ARANLVSLGVAAERAVTEAVKRGVRLAKGIAGIPGLAD"
     gene            6259..>7318
                     /locus_tag="AC159B_00155"
     CDS             6259..>7318
                     /locus_tag="AC159B_00155"
                     /inference="COORDINATES: protein motif:HMM:NF016553.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HIR3376197.1"
                     /translation="MRRLAEVLEAISGTTSKLAKRDLVADYLKTLTDEELRIACTYLT
                     GSSFPAGDGRALNVGWSAIVEALLDVSGASGDALDPAYLRHGDLGAAAFDLLQQRRRP
                     MLFRTPLALPAVDAAFTRMAGATGKGSRQVKIDGMRTLLSNAEPLEAKYLIRIITSDL
                     RVGLKEGLLEEAIAKAFDMPVPTVRRANMLVSDVGMVAMMARHGTLDQATLALFRPFH
                     FMLAETVFSPEEAFASLDPADGAARPSLFVEDKYDGIRAQVHHDGTRLAIYSRTLDEI
                     TPSFPELHADLRALGHQYILDGEIIAWKDGKAIPFSALQQRLRRKDPSDLISSVPVVM
                     FAFDLLLLDGEVLMDRPLS"
BASE COUNT         1358 a         2179 c         2402 g         1379 t
ORIGIN      
        1 gagtatgcaa agttgtgggg attgtcattc atccgactcg tcaaccatct tgtcagggta
       61 atcctcgatg gccagccagt ggagcctctt gcgacgtttc gtgatggcct gcaggtgcag
      121 cggacgatgg ccgccgtgcg cgcgtcggcg cagcgcgggt gggtttcagt cgccagctga
      181 caagaactcc gggggaactt ctcgtacaag gtagatcgac ggcgcggcct gatagaaggc
      241 gaaaccggct tcatgggcgc gtcgcgcaag gaccgtgacg agcacgacct cgtcgctgtg
      301 gcgttttgct accgagatgg catcctggcg cgtcgcagat agatgcacat attgccggcc
      361 gagaggtctc aggccaccga cacggatggc ctcaagtgcc gtccccacgg ttccgtggta
      421 cagccattca ggtggaagca cggaccgtcc cggagtctcg accgggatgc tgtggccgta
      481 ccgtgcgcgc atgcgggtgc catcgatctc gtaccggcgc gggtcgtgct ggacaagacc
      541 ccgaacctcc tgctcggtaa cccaatccca tcccggttga tcggcgacgg ctttgacgag
      601 cgcgtcgacg tcggcgaagc cggatgggtc aagctcgagg ccgatgctct caggcttatg
      661 acgcagcacc agcgcgagga aacgactgag ccgctctgac cggttgcccc catgagatgc
      721 gggccggaaa ggtctttgct gctccatgat gtgagtataa tttacttcgc atatgcccac
      781 gctggttgaa tgcgttccca acttcagtga aggccgccgg caggaagtca tcgacgccat
      841 cgccgacgag gtccggcgga caaaaggcgc gcgtctgctc gatgtccagg cggatgagag
      901 tcacaacagg tgcgttgtga ccttcgtggg accccccgaa gcggcgaaag ccgccgcgct
      961 ggccgcagcg gggcgcgccg tcgagctcat cgatatgaac ggtcaccacg gcgagcaccc
     1021 ccgccttgga gcaatcgacg tcattccgtt cattcctatc tccggcgtta cgatggacga
     1081 tgccgttgcg accgcgcagg ctcttggccg cgaaatatgg gagcgcttgc gcgtcccggt
     1141 gtttttctac gcggaagcgg caacaagacc ggagcgccgc cggctgccgg atattcgtaa
     1201 gggggaatac gagggcctcg cagccaaact caccgacccg gcatggatgc ctgatgtggg
     1261 cgaggcgaaa ccccatccga ccgcgggagc caccgtcgtg ggcgcccgcc gcccgctcat
     1321 cgcctataac atcaacctca gcacggacga catcgaggtc gcgaaaaaga ttgcgcgcgc
     1381 cgtccgcgag agcagcggtg gactggccgc cgtgcaggcg atgggtgtga agagtgaaag
     1441 cggcaaggcg caggtctcga tgaacctcgt ggatttcacg cagacccccg tgcagcgagc
     1501 gtttgaagtg gtcaaggaag aagccgcgca ctacggcgtg accatactcg agagcgaggt
     1561 ggtaggtctt cttcccctcg aggcgatcgt cgacgtcgcc cggcaccacc tccggctccg
     1621 cgacttcacc cgcgagcaaa tcctcgagac ccaccttatg gagtagccgt ggcgcgcttc
     1681 cgcgttcccg cgcttccgcg cttccgtcag tcatgaagca gtccgtcgat ctcctgatca
     1741 cgcatgccgc gcaactgctg accattgctg gaaacaacgg ccatgcccgc agtggggacg
     1801 gcctcgcgga tctgggattg gtcacagacg gggcggtggc ggttgcggat ggtgtgatcg
     1861 tcgccgcagg atcgaccgct caggtcctcg acgaggtcag accatccacg ggctcgacca
     1921 cgatcgatgc ccgcgacgct gtcgtgttgc cgggatttgt ggatccgcat acgcatctcg
     1981 tgtttgccgg atcgcgcgcc gatgagtttg agatgcggct ggccggcgcc acctatcaac
     2041 agatcgcctc ccgaggcgga ggcatcctgt caacgatgcg cgctacgcgc gcagccaccg
     2101 aggatgacct cctggcgctc gggagtctcc ggctggatcg catgcttctt cacggcacca
     2161 caacggttga ggcaaagagc gggtacgggc tgtccgtcga ggacgagttg aagtctctgc
     2221 gcgtcgtcca ccgcctgagc gcgctgcacg atgccgacct cgtgccgacg ttcctcggcg
     2281 cccatgcagt gccgcctgag tttgcgggtg atcccgatgg ttatgttcgg ctggtcattg
     2341 aggagatgat tcccgccgtg gtggaagagg atctcgccga gttttgtgac gtgttctgcg
     2401 acgtgggcgc attcaatccc gatcagtcgc ggagtgtgct cctggcaggg catgaggcgg
     2461 gactcgatct gaagattcac gcggatgaaa tgtctgatca gggaggcgcg cggctggctt
     2521 ccgagctgga agccttttcg gcggaccatc tcctgcgggc atcggaggag gggctgcgcg
     2581 cgatggctga gagcggaacg ctgggtgttc tgcttccggc gacggccctg ttcctcggtc
     2641 tctcctatgc gccggcacgc cgcatgatcg agttgcaggt ccccgttgcg atcggcaccg
     2701 atttcaatcc cggcacgtcg cccacctact caatgccgat ggtgatcgcc ctgtcatctc
     2761 tcggtatgaa gatgactccg gccgaggctc tggtggccgc cacgattaat gccgcccatg
     2821 ccgtcggcat ggcggaagag gtggggagcc tcgagcctgg caaggctgcc gacctcgtga
     2881 ttctcgagat ggatgactat cgacaggttg cgatgacgtt tggcgtcaat ccggtgagaa
     2941 cggtgatcaa acgcgggcgc gttgtcgtcg accgtggacg acttctcaga gaaaaccagt
     3001 gatcagcgaa cctctgacgc gattcctcga ccgtctggct tcggcggaac cgacgccggg
     3061 gggcggcagc gcatccgcgg tcgcgggggc tatggcggcc gccttgctcg caatggtcag
     3121 caggttgacg aaggcgaaag agggcagcgc gtcaattgaa gcagtcgccg gcaagatgga
     3181 tcagggccga aagaccctgc tggatcttgc agcgaaagac gcgcaggcgt ttgaggacgt
     3241 cctgcgggcg atgcgcttgg ctaaggagac cgacgatcaa cgtcgggaac gccagcaggc
     3301 aatccagcgc gcgttgatcg aggccgccgc cgtcccgctc gcggtcgcgg cgcatgcgca
     3361 tgacgtattg aagagcgcgg tgcttgtggc gcgagaagga aacgtgaatg cagtcagtga
     3421 cgccggggtc gccgcgctgc tggtacacgc cgccgtccac ggagcgatcc tcaacgtccg
     3481 catcaacctt tccagcatca aggaccacgg gtacgtcgag gccacagagg cgcgggcgaa
     3541 ggagctgggg agcgcgtccg atcgcctgcg tgatgaggtc atgggaatcg ttcacagcag
     3601 gctggcgcgg ttgggttgat ggccgatcgg atcgtcttgg ccggcatgaa attcttcgcg
     3661 tatcacggcg tgctgccgat tgagcgcagc acgggccagg agtttgtggt cgacgttgag
     3721 ctcgaggctg atctatcgga ggctgcacgc gcagacgatc ttgagcacac cgtggactat
     3781 agccgtgtat acgacatcgt gtgggaaata atgcaggccg agcctcgcca gctgctggaa
     3841 accgtcgcag atgccatcgc acgccaattg ctgaccctcg atcgggtgca ggcagccact
     3901 gtgcgcgtgc gcaagcccca ggttcgactg cccggcccgc tcgactactc cagtgtggaa
     3961 atccgccgtg cacggcgacg ctgatggcca gagtctatat gagtcttggc tctaatcttg
     4021 gggatcgcaa agccactctc gaatcagcgc tgcgacactt gaaggcttcg cgccacgtga
     4081 gcgtgctgcg ggcgtcctct atctatgaaa ccgagcccat tggatatgct gaccagccct
     4141 ggttttacaa ccttgcggcc gaggtcgaaa cagagcttga tcctgacgaa ctcctcgccc
     4201 tgacgaagca ggtcgaacgg gatctgaata ggactcgcca aatccgatgg ggaccacgaa
     4261 cgatcgacat cgacatcttg ctttacgatg atatcgtcgt aaacggcgac cgcttgacgc
     4321 tcccccatcc agaaatgacc cagcgcaggt ttgtgctcga accgttgcac gagatcgcgc
     4381 cggagttgaa gcttcccgac gggcggcgaa tcgcggatct ccttccgaac gtctctgcgc
     4441 aggaggtacg aagggttgca gtctagcgcc atccatgatg ggctgcgcac ccagcgactg
     4501 ggccggccgc taaaggtatt cgagagcgtc ggttcgacga atgacgccgc tcgagagatg
     4561 gccgaggccg gtgcaccgga gggtgtcgcg gttctggcgg gagagcaaac tggaggccgg
     4621 ggtcggaaag ggagtagttg ggcatctcca gcgggaggtc tgtggttatc ggtcgttttg
     4681 cgtcccgacc ttcccctgga agcgtggccc ctgatcggtt tcgcggctgg cgcaggcgct
     4741 gccgcagcgc tcgaatcggt cgccgggcag acggttcaat tgaagtggcc taatgacttg
     4801 atccttgacg gtcaaaaggt cggagggatc ctcgtggaat cagccggggg cttcgccatc
     4861 ctcggcatcg ggatcaacgt gaattttggg aaagacgcgc ttcccgaaga cgtgcggaga
     4921 ggggcaacca ccgttcttgc gtcactcggc cgggaggtag agctggcagt actgacccgc
     4981 gccattcttg agcaagtgga atatttctat gatgtgatac atagggatcc aaaagccgtc
     5041 ttacgcgcct ggcggatgcg ctcgtttctc accggccgtt ctgtgcaagt cagcggcaca
     5101 cgtgtactgg aaggtgtcgc agaagatgtc gacgacagcg gcgcgctgct cattcgtact
     5161 gcatcgggtg ttcaagccgt gactgtgggc gacgtatccg tgcgagagac gcgagcgatc
     5221 taatccatgc cgcagatcac cgacgttccg gggatcaaag tcggtcacgc cacggataag
     5281 agggctgcga ccgggtgcac cgtgattctc tgcgagcggg gcgccgtcgg cggagtcgat
     5341 gtgcgtggcg ccgccccggg cacgcgagaa actgacttgc tccggccgat gaatctcgtg
     5401 gagcgcgtgc atgccgtgtt gctcactggc ggaagcgcgt tcggactcgc tgccgccgac
     5461 ggggtcgtgc ggtttcttga agagcggggc gtcggctttg aaaccggagg cacaaaagtt
     5521 cccgttgtcc ccgcagcggt gatcttcgac cttgcagtcg gcagcgcgaa ggtccgcccc
     5581 gggccggaga tgggctacgc ggccgcccgc gaagctgctg agaaggttga ggaagggtcc
     5641 gtcggcgtcg gcaccggcgc ctccgttgga aaactcctgg gcccggtctc ggccatgaaa
     5701 gggggggtcg gcacatggtc gctccggctg ccgggcggag ttgtgatcgg cgccctcgtg
     5761 gttgtaaatg catttggtga tgtgatcgac accgacaacg gcgcgatcct tgcgggggcg
     5821 cgagatccgg ccaccgggca ttttctcaac atgacagaga cgcttctttc gatggaagaa
     5881 atgccgggtt tcccggggaa cagcactctg gcggtggtcg cgacgaacgc ccggctaacg
     5941 aaggagcaag caaacaaact tgcgcaactt gcccatgacg ggctggccag ggtcatcact
     6001 ccggcccata cgatgtttga cggggatacg gtatttgcgc tctcgacggg tgaggcaaga
     6061 gccaacctag tgtccctagg cgtggcggcg gaacgggccg tcacggaagc ggttaagcgg
     6121 ggcgttcggc tggcaaaagg aattgccggc ataccgggtc ttgccgactg aacctccaac
     6181 gaacttgtcg ctgagggggc caggctccat cgcatgcaag tttcaagcaa gcttcgtcgg
     6241 gtaggctaaa atggaatcat gcgcaggctg gccgaggtcc tggaggccat ttccggcaca
     6301 acaagcaagc tcgcaaaacg cgaccttgtg gccgactacc tcaagaccct gaccgacgaa
     6361 gagttacgta ttgcctgcac gtaccttacg ggttcctctt ttccagccgg cgatgggcgc
     6421 gcgttgaatg ttggctggtc cgcgatcgtt gaagcgctgc ttgacgtgag tggtgccagc
     6481 ggagatgccc tggacccggc ctatctgcgc cacggggatc ttggcgccgc ggccttcgat
     6541 ctgctgcagc aaagacgacg gccgatgctg tttcgaacac ccctcgcgct tcccgcagtg
     6601 gacgccgcgt tcacgcggat ggccggcgcc accggcaagg gatcgcgcca ggtgaagatc
     6661 gacgggatgc gcacgttgtt gagcaacgcg gaacccctgg aagcgaagta cctgattcgc
     6721 ataatcacgt cggacttgcg tgtcggattg aaagagggtc tcctcgaaga ggccatcgca
     6781 aaagcgttcg atatgcccgt ccccaccgtg cgccgcgcca acatgctggt cagcgacgtt
     6841 ggtatggttg caatgatggc ccggcacggg accctcgacc aggccaccct tgccctattc
     6901 cgtccgtttc acttcatgct ggcggagacg gtctttagcc ccgaagaggc gttcgcctcg
     6961 ctggaccccg ccgacggcgc ggcgcggcct tctttgttcg tcgaggacaa gtatgacggc
     7021 atccgcgccc aggtccacca cgatgggacg cgactggcca tctattcgcg aacgctcgat
     7081 gagattacac cgtcgttccc cgagcttcat gccgatttgc gcgcgctcgg acatcagtac
     7141 atcctggacg gcgagatcat tgcctggaag gatgggaaag cgatcccgtt ctccgccctg
     7201 cagcaacgcc ttcgtcgcaa ggatccctcc gacctcatca gctccgtacc ggtcgtgatg
     7261 tttgcgttcg atctcttact cctcgacggc gaagtcctca tggaccgtcc actgtccg
//
LOCUS       DBNGSB010000005         4704 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3225097, whole genome shotgun sequence.
ACCESSION   DBNGSB010000005 DBNGSB010000000
VERSION     DBNGSB010000005.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 4704)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4704)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4704
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3225097"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1238
                     /locus_tag="AC159B_00160"
     CDS             <1..1238
                     /locus_tag="AC159B_00160"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004344.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HIR3376198.1"
                     /translation="SFASLGDVVIAEPAAQIGFVGPRVLGLTAGEHPPADSHRAESRL
                     RSGLVDLVVPRRQLRDVVAYLVSHLGPTPGIRRRAAARKVIRRRRRAPAIPSWPEVQL
                     ARHPGRPTSADYIERVFARVVELHGDRTYGDDPAIITGLGELDGQTVVIVAQERGRTE
                     EEQRFRRRGMPMPEGYRKAQRVMQLGAKFRLPVIALVDTPGASSGAESEQRGIAQALA
                     RTLQAMAILPTASISVVIGEGGSGGAISLGVADRVFMLEHAIYSVISPEGAAAILWRD
                     TSKAADVANALQITASDLLRLGVIDGIIPEPTGGAHTDPDLAATHLRNALLAAVAALR
                     RVPLSKLLRRRYDKFRNIGRVGIYWRQVVRTEMQELLEALGQRILRREQEGRQEERGE
                     AGRKRISDLTTGGETEPQS"
     gene            1323..2636
                     /locus_tag="AC159B_00165"
     CDS             1323..2636
                     /locus_tag="AC159B_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HIR3376199.1"
                     /translation="MVSLGFQHVLTMFPATVLVALLTGFDPGVTLFASGLATVLALLL
                     SGMRIPMYYGSSFSYIAAIRGVTVPVGASAPNPALVPLAQVGIIGTAIVNVIAGFIVR
                     AVGKARLDKVLPPVVTGSVAVVIGIALGKAALDLATGTCCNPAAVSEWWTVAIITLLV
                     TIIFSVYLRGRGLIGMLPVLLGAVVGYVVAWIFGLVNFAPVGEAAWLRVPDFSFPAFG
                     DPKAWATIFAIAPIAIATIPESTAHLYQISLYIDRVATELRREKLEVKKLVGLNLILD
                     GVGDGVNGLLGGCAGTNYGENNSLMVITRNYSGPVLVAAGVIAMLLGFVGKLAALVQT
                     LPPAVTGGLAIYLFGVIGLQGVALMMAEGVDFFDPRQLAIGAAILVIGIGGAAFPGGN
                     IPIWGWQLPSIATSAMAGILINLIFLLFPPERYVPAAMGTMGTGR"
     gene            2774..3328
                     /locus_tag="AC159B_00170"
     CDS             2774..3328
                     /locus_tag="AC159B_00170"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3376200.1"
                     /translation="MDEAELVARSQSGDTDAYDQLVGAYQHRIFSLAYRITGNREDAW
                     DAAQEAFLKAYRNLRGFHGRAAFLTWLHRIAVNAALDVVRRRPAQPPVPLDVSLKSLD
                     DPADEVMRSDTQQRVHRAIARLPVEQRVAIVLRDLHGLRYDEIAGVLQIPIGTVRSRI
                     SRGREMLRITLADFAPASGEGRTA"
     gene            3328..3894
                     /locus_tag="AC159B_00175"
     CDS             3328..3894
                     /locus_tag="AC159B_00175"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR3376201.1"
                     /translation="MRSMHVTDALSAYVDGALREGERTQVERHLETCEDCRRHLSALR
                     DLVATVRSAEPVAAPEGFRAQVRARVEQMDPRPDVARRWPSIPLPWRVVSAAAAVLGI
                     GIFAASLLRTQGPAAMRELDKPGESQIASGDRLRSAAPSAPEVAQRTIPKQRPSVVRG
                     IVLIALPVLALSTLAWIAIRRRRSRRAW"
     gene            complement(3910..4380)
                     /locus_tag="AC159B_00180"
     CDS             complement(3910..4380)
                     /locus_tag="AC159B_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376202.1"
                     /translation="MHQARLAVLIAITLVFGVSSVAYAPYHTQPARPPSTPAPSGMSV
                     VKAQAKIAGTHAGFAAGSATMSSAKEHLAHVIACIEGSKGRNVKPQWMNPCSGNGNGV
                     LVDLRRIKASASLIRAAQDADATAVAAIRNSNLTQVKNSARRVGQLTNRIATAK"
     gene            complement(4513..>4704)
                     /locus_tag="AC159B_00185"
     CDS             complement(4513..>4704)
                     /locus_tag="AC159B_00185"
                     /inference="COORDINATES: protein motif:HMM:NF022834.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxamate carbamoyltransferase subunit AllH family
                     protein"
                     /protein_id="HIR3376203.1"
                     /translation="SEAYLMAAARGEAGEAWHRLLRVLPNGDRAAIASATRRVMAFGE
                     TSGSDMLAGFTVAFSTLIS"
BASE COUNT          782 a         1473 c         1545 g          904 t
ORIGIN      
        1 cgagcttcgc gtcgctcggc gacgtcgtga tcgcggagcc ggcggcgcag attggattcg
       61 tcggtccacg ggtcctggga ttgaccgcgg gcgagcatcc tcccgcggac tcgcatcggg
      121 ccgaatcgcg actccgatcg ggactggtgg acctcgtcgt tccccgtcgc caactgcgcg
      181 atgtcgtcgc ctacttggtc tcgcatctcg gacctacgcc gggaatccgg cgtcgtgcgg
      241 cggcgcgcaa ggtgatcaga cgcagacgca gagcgccggc cattccttcg tggcccgaag
      301 tgcagctggc gcgtcatccc ggccgcccca catccgctga ctacattgaa cgcgtgttcg
      361 cgcgcgtggt agagctgcac ggcgatcgaa cgtatggcga tgaccccgcg atcatcaccg
      421 gattgggtga gctcgacggc caaaccgtgg tgatcgtcgc ccaggaacga ggccgcacgg
      481 aagaggagca gcggttccgc cgccgaggca tgcccatgcc ggagggctac cggaaggccc
      541 agcgggtgat gcagctgggc gcgaagttcc gcttgccggt gatcgcgctc gtcgacactc
      601 ccggcgcatc gtcgggcgcc gaatcggagc agcgcgggat cgcccaggcc ctcgcacgca
      661 cgcttcaggc catggccatc ctgccgaccg cgagtatcag tgtggtgatt ggtgaaggtg
      721 gcagcggcgg ggcgatctcg cttggtgttg ccgaccgcgt gttcatgctc gagcatgcca
      781 tctactcggt gatctcgccc gagggtgcgg ccgccatcct gtggcgcgac accagcaagg
      841 cggccgatgt cgcgaatgcg ttgcagatca cggcgtccga cctgttgcgt ctcggcgtca
      901 tcgacggcat tattcccgaa cccacggggg gcgcgcacac cgatcctgac ctcgccgcca
      961 cccacctgcg caatgcgctg cttgccgctg ttgcggcgct gcgccgcgta ccgctctcga
     1021 aactgctccg gcgacgctac gacaagttcc gcaacatcgg ccgggtgggg atctactggc
     1081 gtcaggttgt gcggacggag atgcaggagc tgctggaagc attgggacag cgcatcctgc
     1141 gccgtgagca ggaagggcgt caagaggaaa gaggcgaggc cggccggaaa cgcatttcag
     1201 acctaaccac gggaggtgag accgagccgc agagttgaca gattgtaaca ctcaggaggt
     1261 gcattgtggc cgaacgagct ccgtacgcat ttctacctcc cgaccgtccg ccagttggcg
     1321 cgatggtcag tcttggattc cagcacgtcc ttacaatgtt tcctgcaacc gtcctcgttg
     1381 cgcttcttac cggattcgat cctggagtga cgctgttcgc gtcgggcctg gcgacagttc
     1441 tcgccttact gttgtcggga atgagaattc cgatgtacta cggcagcagt ttctcataca
     1501 ttgccgccat tcgcggcgtt accgtgcctg tcggcgcgtc cgcgccaaac cccgcgctcg
     1561 tgcccctggc gcaggtgggg attatcggga ccgctattgt gaacgtcatt gccggattca
     1621 ttgtccgcgc cgtcgggaag gcgcgactgg acaaagtgct gccgcccgtc gttaccggct
     1681 ccgtggccgt cgtcatcggg atcgccctgg gcaaggcggc gctggacctg gcgacgggca
     1741 cttgctgcaa cccggctgcg gtcagcgagt ggtggacggt ggcgattatc acgttgctcg
     1801 tcaccatcat cttctccgtg tatctgcgcg gccgcggtct catcggtatg ctgccggtgt
     1861 tgctgggggc ggtcgtcggc tatgtcgtcg cgtggatctt cggtctcgtg aacttcgcgc
     1921 cggtcggcga agcggcctgg ctccgcgttc cggacttctc cttcccggcc ttcggcgatc
     1981 ccaaagcgtg ggcgacgatc ttcgccatcg cgccgatcgc gatcgccacg attcctgaat
     2041 caacggcaca cttgtaccag atcagcctgt acatcgaccg ggtggcaacc gagttgagac
     2101 gggagaagct tgaagtcaag aaactggtcg gcctgaactt gatccttgat ggcgtcggcg
     2161 acggtgtgaa cgggctgctc ggcggctgcg ccgggacgaa ctatggcgaa aataactcgc
     2221 tgatggtcat cacacggaac tactcgggac ctgtgcttgt tgcggcgggc gtgatcgcga
     2281 tgttgctggg gtttgtcggg aaactggccg cgctcgtcca gacgctcccc ccggcagtga
     2341 cgggcggact ggcgatctac ttgttcggcg tcatcggatt gcagggcgtg gctctcatga
     2401 tggccgaagg cgtggatttc ttcgatccgc gccagttggc gatcggggcc gcgatcctcg
     2461 tcattgggat cggcggcgct gcatttccag gtggcaacat cccaatctgg ggatggcagc
     2521 tcccatcgat cgccacctca gcgatggccg gcatcctcat taacctgatc ttcctgctgt
     2581 ttccgccgga gcggtatgtc cccgcggcaa tgggtactat gggcacgggc cgctagaccc
     2641 gtctgtgggg cgagggccgg gattcgtccc ggccctcttt gtttgggaac tttcaaggcc
     2701 cggcgccgtc taacaagcag aacaagacgg caggcaatgc tgtgtggacc tcacgccgcg
     2761 gggtggaacc tcggtggatg aagcggagct tgttgctcgc agccaaagtg gtgatacgga
     2821 cgcctacgat caactcgttg gcgcctatca gcatcgcatc ttttcgctgg cctaccgtat
     2881 tacggggaac cgcgaggatg cgtgggatgc agcgcaggag gcgtttctga aggcgtatcg
     2941 gaacctccgc ggctttcacg gtcgcgctgc atttctgacc tggctgcacc gtatcgcggt
     3001 gaacgcggcc ctcgacgttg tccggcggcg gccggctcag ccaccagtgc cgctcgacgt
     3061 atcgctgaag tccctcgatg acccggccga tgaggtcatg cgcagcgaca cgcagcagcg
     3121 tgtccataga gcgatcgccc gactaccggt tgagcaacgc gtcgcgatcg tgctgcgcga
     3181 ccttcacgga ctgcgctacg acgagatcgc cggggtgctg cagattccga ttggaacagt
     3241 ccggtcgcgc atcagccgtg gccgtgagat gctgcgaatc acgctggcag acttcgcgcc
     3301 cgcgagcgga gaggggagga cggcgtaatg cgatccatgc atgtgaccga cgccctgtcg
     3361 gcgtacgttg acggcgctct cagggagggc gagcgaactc aagttgagcg tcatctggaa
     3421 acttgtgagg actgccgccg tcatctgtcc gcgcttcgag atctagtggc gaccgtgcgg
     3481 tcggccgagc ctgtggccgc ccctgaagga tttcgcgcgc aggtgcgggc gcgggtggag
     3541 cagatggacc ctcgcccgga tgtggcacgg cgttggccga gtatcccgtt gccatggcga
     3601 gtggtcagcg ccgccgcggc cgtgctcggc atcggcattt ttgccgcaag tctgctgcgc
     3661 acgcaggggc cggcggccat gcgtgaactc gacaagccag gcgagagtca gattgcgagc
     3721 ggagatcgat tgaggtccgc agcgccatcg gcgccggagg tagcgcagcg gacaatcccc
     3781 aagcagcgtc catccgtagt tcggggcatc gtactaatcg cactgccggt cctggcactg
     3841 agtacactgg catggatcgc tattcgacgc cggcgatcgc gcagagcctg gtgagcgacg
     3901 ccagcgccgc tatttcgctg ttgcgatccg attagtcaac tgcccaacgc gcctggcgct
     3961 gttcttgacc tgcgtgagat tgctgttcct tatcgctgcc acggccgtcg cgtcggcatc
     4021 ctgtgccgcc cggatgagcg atgctgaagc cttgatccgc cggagatcta ccagcacgcc
     4081 gttcccattg ccgctgcacg gattcatcca ttgcggtttg acgttccgac ctttggaccc
     4141 ctcgatgcac gcgatgacat gcgcgaggtg ctccttggcg ctgctcatgg tggcgctccc
     4201 cgcggcaaac cctgcgtgcg taccggcaat ctttgcctgc gctttgacga cgctcatccc
     4261 cgacggcgcc ggcgtgctcg gcggccgcgc cggttgcgtg tgataaggag cgtacgcgac
     4321 cgaagagacg ccgaacacta acgtgatggc gatgaggact gccagcctgg cctgatgcat
     4381 acgatcacct cgatcgattt cctaagcaca ttctggccgc tccatttcac accagtgtaa
     4441 cgggcgcgga gaacgcgaag agacataacc gccagatagc cttggagtcg tgcccagagg
     4501 tggccgagca cttcaggaaa tcagcgtgga aaaagccacc gtgaagccgg cgagcatgtc
     4561 cgacccggat gtttccccga acgccatcac ccggcgagtc gctgaagcaa tggccgcccg
     4621 atccccgttg ggaagcactc gcaagagccg gtgccacgcc tctccggctt ctccgcgagc
     4681 cgcggccatg aggtatgctt cgct
//
LOCUS       DBNGSB010000006         4465 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2304815, whole genome shotgun sequence.
ACCESSION   DBNGSB010000006 DBNGSB010000000
VERSION     DBNGSB010000006.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2304815"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..702
                     /gene="rimI"
                     /locus_tag="AC159B_00190"
     CDS             184..702
                     /gene="rimI"
                     /locus_tag="AC159B_00190"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 - protein-N-terminal-alanine
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HIR3376204.1"
                     /translation="MKQLVRVTVESMSSADIPRVLEIERESFRTPWPRDAYVHELKEN
                     RLATYFIAKSGEQIVGYCGMWVILDEAHITTIAVDPEFRGMHVGERLLIGLIDASVDR
                     GARWMTLEVRRSNLTAQKLYKKYGFREIGTRKAYYSDNREDAIIMWTGSLLDTSFKDR
                     LVHLRAELERSE"
     gene            699..1730
                     /gene="tsaD"
                     /locus_tag="AC159B_00195"
     CDS             699..1730
                     /gene="tsaD"
                     /locus_tag="AC159B_00195"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIR3376205.1"
                     /translation="MIVLGIETSCDETAAAVVEDGRRICSNVVASQVQLHRRFGGVVP
                     ELASRKHVERLLPVLDESLEQAGVPLTRIEGIAVTHGPGLVGALTVGVAAAKGLALSL
                     DRQLIGINHLEGHIYAAFLAEPDIEFPVLALIVSGAHTDLVAMSAHGSYGVLGRTRDD
                     AAGEAFDKIARAIGLPYPGGPELDTLGRQGDHRKVPLPLPMPNGSLDFSFSGLKTAAL
                     LALRKSELSDGQWKMDLAASVQHVVAKVLVSKTMAAAERARPRTVILVGGVAANSVLR
                     AMMQQACSGKGYRLVIPPPILCTDNAAMIAAAGDYRFSRGLYDDLALSAYSDLPLGPR
                     SERSADLRA"
     gene            2042..2356
                     /gene="groES"
                     /locus_tag="AC159B_00200"
     CDS             2042..2356
                     /gene="groES"
                     /locus_tag="AC159B_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIR3376206.1"
                     /translation="MNMRPLGDRVVVKPLEEEERSKGGIVIPDTAKEKPQHGEVVAAG
                     PGDWDQKGEKRIPLDVKAGDRVLYAKYSGSEVKIDGQEYLILRHSDILALVERQPAGV
                     KK"
     gene            2459..4063
                     /gene="groL"
                     /locus_tag="AC159B_00205"
     CDS             2459..4063
                     /gene="groL"
                     /locus_tag="AC159B_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085406.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR3376207.1"
                     /translation="MLAYNETARRAMERGVNKLADAVKITLGPKGRNVVLEKKFGSPT
                     ITHDGVTVAKEIELEDPFENAGAQLVREVATKTEDVAGDGTTTATILAQSLVRDGFKM
                     VAAGSNPMSLKRGIDKAVAVAIEEIRKVSKPVETKEAIQSVASISANDSDIGALIADA
                     MDKVGKDGVITVEESKGIETTVEVVEGMMFDKGYISPYFDTDPEKMEAVLEDPFILIT
                     DKKISAVKDLLPVLEKIAQVNRPLLIIAEDLEGEALATLVVNKLRGTLHSVAVKAPAF
                     GERRKAILEDIAILTNGQVISEEIGLKLESIDLSKLGRAKQVRIRKEETIVVGGGGAQ
                     DAIQKRVGQLRKQIEDTDSDYDREKLQERLGKLSGGVAVIKVGAPSEVELKYRKTRTE
                     DALRATRSAVEEGVVPGGGSVLVQIVKALEKIEAEGDEKIGVGLVSKALLEPAHQLAV
                     NAGQEGWLIVEQQKEQKPGIGYNVLSGKFEDMSKAGIVDPAKVVRSALQNAASIASLL
                     LTTEAMVVEKPEEDEKTPAMPQMPPM"
     gene            4184..>4465
                     /locus_tag="AC159B_00210"
     CDS             4184..>4465
                     /locus_tag="AC159B_00210"
                     /inference="COORDINATES: protein motif:HMM:NF014857.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376208.1"
                     /translation="MLVGPDGISWLHCPHLDPKSEHSEGRLRVFVVGAGGREHALVWK
                     LRQGRTPAQVYAAPGNPGIARLARCLPIDSTDIQALADAASELSVDLTIV"
BASE COUNT          974 a         1267 c         1410 g          814 t
ORIGIN      
        1 gccacgggcg cagtggatgc cacacgccgt gcacgtcggc cggatcggat ggctgcgatt
       61 ggcgcggggc gagcacgatg acctctacca gatactgccc gtctacgccc gtgtccccgt
      121 cacctagaaa gcgggaacag ggaactagga aacaggggaa cgaggtaaga atcacacttt
      181 ggtatgaagc agctggtccg cgtcacagtt gaatcgatgt catcggccga catcccgcgg
      241 gtactggaga tcgagcgcga gtccttccgg acgccgtggc cgcgcgatgc ctatgtccac
      301 gagctgaagg aaaaccgctt ggccacgtac ttcattgcca agtctggcga gcagatcgtc
      361 ggctattgcg ggatgtgggt gattcttgac gaggcgcaca tcacgaccat cgcggttgat
      421 ccggagtttc gcggaatgca tgtcggcgag cggctgctca tcggactgat tgacgcctcg
      481 gtcgaccgcg gcgcccgatg gatgaccctc gaggtgcgcc gttctaatct gaccgctcag
      541 aaactgtaca agaagtatgg attccgtgag atcggcaccc gcaaggcgta ttacagtgat
      601 aaccgtgaag acgcgatcat catgtggacg ggcagtctgc tagacacgtc cttcaaggat
      661 cggctcgtcc acctccgggc agaactcgaa agaagtgagt gatcgttctc gggattgaga
      721 cctcctgtga cgagacggct gccgcagtcg ttgaagatgg tcgcaggatt tgcagcaacg
      781 tggttgcctc gcaagttcag ctccaccgcc gcttcggcgg cgtagtcccc gaactcgcat
      841 cccgcaagca cgtcgaacgg ctgttgcccg tactcgatga gtcgctcgag caggcgggag
      901 tcccattgac ccgcattgag ggcatcgccg taacacacgg accgggcctg gtcggagccc
      961 tgacggtcgg cgtggccgca gccaaagggt tggcgctcag tctcgaccgc cagctgattg
     1021 ggatcaacca ccttgaaggc cacatctacg ccgcgtttct tgccgaacca gatatcgagt
     1081 tccccgtgct tgcgctcatc gtctccggcg cgcacaccga cctcgtcgcg atgagcgcgc
     1141 atggctcata cggcgtgctg ggccggacgc gcgatgacgc cgccggggag gccttcgata
     1201 agattgcccg tgcgatcggg ctgccgtatc ccggtggtcc agagctcgat actctgggcc
     1261 ggcaaggtga tcacaggaaa gtgccactgc ctctgccgat gccgaatgga tcgttggatt
     1321 tctcgttcag cggattgaaa actgccgccc ttctggcact taggaaatct gagctttcag
     1381 atggtcagtg gaagatggac ctggcggcaa gcgtccagca tgtcgtggca aaagtgctcg
     1441 tgagcaagac tatggccgcg gccgagcgag cgcgtccacg gacggttatc ctcgtcggcg
     1501 gcgtggcggc gaacagcgtt cttcgggcca tgatgcagca ggcgtgcagc ggcaaggggt
     1561 accgcctggt catccctcca cccatcctct gcaccgataa cgccgccatg atcgctgcgg
     1621 caggtgacta ccgcttcagc cgaggactat acgacgacct ggcgctctct gcctacagcg
     1681 acctgccctt gggcccaaga agcgaacgat ctgccgacct aagggcctaa catccgggcg
     1741 tcctggggct ccctggggac aggcccctgt ggataatgtg aataaccctg tgagtaaagc
     1801 gtcttgtact gctttttgga agagtctacc ggcggcactt acccgatggc ggaaattcgt
     1861 gtagaatcga gacttggcag ggtcccaaca ccaaaaccag ggaatctggc catttgtcgg
     1921 cgtctcgtag agctggaggg cttgatagct actcggctct tccataagat ggaaacggtc
     1981 tagcactctc tgtcagagag tgctaatccc cgaatctgca taccgtgaag gaggaagaac
     2041 catgaatatg cggcctcttg gcgaccgcgt ggtggtcaag ccgctcgagg aagaggagcg
     2101 ctcgaagggt ggcatcgtca tacccgatac ggcgaaggag aagccacagc acggtgaggt
     2161 tgttgccgcc ggcccaggcg actgggatca aaaaggcgaa aagcgcatcc ctctcgatgt
     2221 gaaagccggt gaccgcgtgc tctacgcgaa gtattcaggg agcgaggtca agatcgacgg
     2281 gcaggagtat ctgatcctgc gccacagcga catcctcgcc ctggtggagc gccagcccgc
     2341 aggcgtaaag aaataagaac gggacgaagc gacccgcgat caagcgacca cagtggtcgc
     2401 gtggtctcag gtcccatggt cccgtacttt gtgtggaggt gaatagagag tgccaaagat
     2461 gctcgcctat aacgagacgg cccggagggc gatggagcgc ggggtcaaca agctggccga
     2521 cgccgtgaag atcacgctcg gaccaaaggg ccgcaacgtc gtgctcgaaa agaaatttgg
     2581 ctcaccgacc atcacgcatg acggcgtgac ggtggcaaag gagatcgaat tggaggatcc
     2641 ctttgagaac gccggcgcgc agctcgtgcg ggaggtcgcg accaagaccg aagacgtggc
     2701 cggcgacggt accacgaccg cgaccatcct ggctcaatca ttggtgcggg acgggtttaa
     2761 gatggttgcg gcaggctcaa acccgatgtc gctgaagcgc ggcattgaca aggccgtcgc
     2821 ggtcgcaatc gaagagatcc gcaaagtcag caagcccgtt gaaactaaag aggcgattca
     2881 gtccgtcgcc agtatctccg ctaacgattc cgacatcggc gccttgattg cagacgccat
     2941 ggacaaagtc ggcaaggatg gcgtcatcac ggtggaagag agcaagggaa tcgagacgac
     3001 cgttgaggtc gtcgaaggca tgatgtttga taaaggctat atctctccgt acttcgacac
     3061 cgatcctgag aagatggaag cagtcctcga agatcccttc atcctaatca ccgataagaa
     3121 gatctcggcg gtcaaagatc tcctcccggt cctggagaag atcgcgcagg tcaaccggcc
     3181 gcttctgatc atcgcggagg atctggaagg cgaggcgctc gcgacactgg ttgtcaacaa
     3241 gctgcgaggc acgctgcatt ccgtggccgt gaaggcgccc gcgttcggtg aacggcggaa
     3301 agcgatcctc gaagacatcg ccatcctgac gaatggccag gtgatctccg aagaaatcgg
     3361 cttgaagctg gagtcgatcg atctttccaa actcggtcgc gccaagcagg tgcgcatccg
     3421 caaggaagag acgatcgttg tcggcggcgg cggcgctcag gacgcaatcc agaagcgcgt
     3481 cgggcagctc cgcaagcaga tcgaagacac tgattcggac tacgatcgtg agaagctgca
     3541 ggaacggctc gggaagctct ccggcggcgt ggccgtgatc aaggtcggcg cgcccagcga
     3601 ggtagagctc aagtaccgca agacccgcac tgaggacgcg ctgcgcgcaa cgcgttccgc
     3661 agttgaagag ggggttgttc cggggggcgg cagtgttctg gttcagatcg tcaaagccct
     3721 cgagaaaatt gaggccgaag gtgacgagaa gattggcgtc ggcctggtct ccaaggcgct
     3781 gcttgaaccc gcccaccagc tggcagtcaa cgccggtcag gaaggctggc tcattgttga
     3841 gcagcagaaa gagcagaaac ctgggatagg ctacaacgtg ctgagcggga agttcgaaga
     3901 catgagcaag gccggcatcg tggatccggc caaagtcgtc cgctcggctc ttcagaacgc
     3961 tgcgagcatc gcgtcgctcc ttctcactac ggaagcaatg gtagtggaga agcccgaaga
     4021 agacgagaag acgccggcga tgccgcagat gccgccgatg tagacttacg ggcaaggtag
     4081 cgtcaaacgg gctccggttc accacccgga gccttttgat ttgttgcagc gagcctacaa
     4141 gaaaacacga acgttcttgc cgcatgctta cagatcgtat agaatgctgg ttggcccgga
     4201 tgggatttcg tggctgcatt gccctcatct ggacccgaaa tccgaacatt ccgagggcag
     4261 attgcgagtt tttgttgttg gtgccggggg cagggagcat gccctggtgt ggaagctccg
     4321 ccagggtaga accccagcac aagtctacgc cgcgcctgga aacccaggta tcgcccgtct
     4381 agcccgctgc cttcccattg actccacgga cattcaggcc ctggccgatg ccgcgtcgga
     4441 gctctcagtt gacttgacca tcgtc
//
LOCUS       DBNGSB010000007         3615 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2152224, whole genome shotgun sequence.
ACCESSION   DBNGSB010000007 DBNGSB010000000
VERSION     DBNGSB010000007.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2152224"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..87
                     /locus_tag="AC159B_00215"
     CDS             <1..87
                     /locus_tag="AC159B_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015236938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3376209.1"
                     /translation="DAKITEIYEGTSEIQRLVIARSMIETMS"
     gene            212..979
                     /locus_tag="AC159B_00220"
     CDS             212..979
                     /locus_tag="AC159B_00220"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HIR3376210.1"
                     /translation="MNIVVCVKQVPDTEAPIRIKPDGTGIDETGLNLVMNYYDEHAIE
                     AALRLKEQFGGTVTLIAAGPERTKEALRTGLAMGADEAMLITGPGLEQADHLGTARVL
                     AAAIKALPHDLVICGKLSTDDNAGIVGPALAELLGLPQATAIAHLEIDASATMALVHR
                     EVEGGVEILEVPLPALLTVERSLNEPRYPSLPGIMKAKRKEIKASTPSDPKVDASTRL
                     TKLVPPPKRQAGRVIEADAEKAVAEIVAFLKNEAKVL"
     gene            983..1981
                     /locus_tag="AC159B_00225"
     CDS             983..1981
                     /locus_tag="AC159B_00225"
                     /inference="COORDINATES: protein motif:HMM:NF013200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HIR3376211.1"
                     /translation="MANDVWVVVEHEGGQPKKISLELLGRARELAQALGGKTVALVPG
                     KDVSAVARALGPYGADIVYTVQDPLLEPYTADAQTQVVGEALRKEQPTLVLIGSTAQG
                     RDYAPRVAARIGAAIVTDCARLEVDGGRLVATRPMYTRKAIGTVSFSSDGIRIAVILP
                     AVYPVPHADPKRTAEVRAMQVSIDAKQIRTKVREVQAIKRETVPLTEADVIVSGGRGL
                     RGPENFALLDALAKALGAAVGSSRPPVDSGWVPHDYEIGQTGKTVSPQVYLACGISGA
                     PQHLAGMSGSKHIVAINKDPNAPIFQLADLGVVGDLFEILPKLTEAVRQAKGGNRD"
     gene            1985..>3615
                     /locus_tag="AC159B_00230"
     CDS             1985..>3615
                     /locus_tag="AC159B_00230"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA mutase large subunit family protein"
                     /protein_id="HIR3376212.1"
                     /translation="MEKLWQLRQEKQRWEATTLKEALKRQPERANQFSTLSDLPVNRL
                     YGPDDLKGFDLERSLGHPGEYPFTRGIHPTMYRGRLWTMRQFAGFGSAEDSNRRFHYL
                     LEKGQTGLSVAFDMPTLMGYDSDHPRALGEVGREGVAVDSVQDMELLFRDLPLDRVTT
                     SMTINAPANILLAMYVAAAENMGFTRAQIGGTTQTDMLKEFIAQKEWIVPPRPSMKLI
                     QDMLVFGTRELPRWNVISISGYHIREAGATAVQELAFTLADGIAYVQAGIEAGLDVDE
                     FAPRLSFFFDCHNDFFEEVAKFRAARRLWARIMRDRFQAKNPRSWLLRFHTQTAGVSL
                     TAQQPLNNIVRVAVQALAAVLGGTQSLHTNSMDETYALPTEDAVMVALRTQQIIAHES
                     GVADTVDPLGGSYFVESLTTTMEERAEAYIQKIDDLGGMVRAVELGYPQREIAEASYH
                     YQQQLERKEKIIVGVNEFVQPEEQRIPILRIDPEVERNQIERVRRVRAGRDEVKARRA
                     LDEVKRAADSGENTMYKVLECVKVGGTMGEICDVFR"
BASE COUNT          741 a         1093 c         1161 g          620 t
ORIGIN      
        1 gacgccaaga tcacagagat ttacgagggc acgtccgaga tccagcggct ggtgatcgca
       61 agaagcatga tagaaacgat gtcgtgaatg gtgattggcg agtgagaacc cggccagcta
      121 tcgggggctc cgaggcctct ccccaaacta cgactaccac ggggacccct ctagcctcgg
      181 agcccccgtc cctccccggg ttcatcattc gatgaacatc gtagtctgtg tgaagcaggt
      241 gccggacacc gaggctccga ttcgcatcaa gccggacggc accggcatcg atgagaccgg
      301 tctcaatttg gtcatgaatt actatgatga gcacgcgata gaggccgccc tgcgactcaa
      361 agagcagttc gggggaaccg tgacgctgat cgcggcgggc cccgaacgca cgaaagaagc
      421 cctccgtacg gggttggcca tgggcgccga cgaggcgatg ctcattaccg gcccaggtct
      481 cgagcaggcc gaccacctgg ggacggcccg cgtgctcgcc gccgcaatca aggcgctgcc
      541 ccacgacctg gtcatctgcg gcaagctcag caccgatgat aacgcgggta tcgtgggtcc
      601 ggcgctcgca gaactgctcg gcctgccgca ggcgacggcg attgctcatc tcgaaatcga
      661 cgcgtcggct accatggcgc tcgtccaccg agaggttgaa ggcggggtcg agatactgga
      721 ggtaccgctt cccgcgttgc tgaccgtcga gcgcagcctc aacgagccac ggtacccatc
      781 actccctgga atcatgaaag ccaagcgcaa ggagatcaaa gcatcaacgc cctcagatcc
      841 taaagtagat gcgtccacca ggcttaccaa actggtgccg ccgcccaagc gtcaggcagg
      901 tcgtgtgatc gaagcagacg cggagaaggc cgtcgctgag attgtcgcct tcctcaagaa
      961 cgaagcgaag gtgctgtaag agatggcgaa cgacgtctgg gttgtcgttg agcacgaagg
     1021 cggtcaaccc aagaagatta gtcttgagct gttgggacgt gcacgcgaac tggcgcaagc
     1081 gctgggcggg aaaacggtcg cgctcgtccc gggtaaggac gtctccgccg tcgcgcgcgc
     1141 gctcggtccg tacggtgccg atatcgtata cactgtccag gatccgctac tcgagccgta
     1201 tacggcggac gcgcagaccc aggtcgtggg tgaggcgctg cgcaaggagc aacccacgct
     1261 cgtgctcatc ggctccaccg cacaggggcg cgactacgcg cctcgtgtcg cggcaaggat
     1321 cggcgccgcg attgtgaccg attgcgccag gctcgaagtc gacggcggcc gcctggtggc
     1381 gacacgtccg atgtacacgc gcaaggcgat cggcacggtt agtttttcgt ccgatggcat
     1441 tcgcatagca gtgatcctgc cggctgtcta tcccgtgccg cacgcagacc cgaagcgaac
     1501 cgcagaggtg cgcgcgatgc aagtctcaat cgacgcaaag cagatccgca cgaaggttcg
     1561 cgaggtccag gcgatcaagc gggagaccgt gccgctgacg gaagcggatg tcattgtctc
     1621 cggcgggcgg ggtctccgag gccctgaaaa ctttgcattg ctcgatgcgc tggcgaaagc
     1681 gctcggggcg gcggtcggct catcccgccc gccggttgat tcaggctggg tgccgcacga
     1741 ttatgagatc ggtcaaaccg gcaagaccgt gagcccgcag gtctacctgg cctgtggaat
     1801 ctcaggcgcg ccgcagcatc tggcaggtat gtcgggctcg aagcacatcg ttgccatcaa
     1861 caaggatccg aatgcgccca tttttcaact cgccgacctc ggagtggtcg gcgatctctt
     1921 cgagatcctg ccgaagctca cagaggccgt gagacaggcg aaaggcggga atagggatta
     1981 agggatggag aaactctggc agttacggca agagaagcag cgttgggaag cgaccacgct
     2041 caaagaggcg ttgaaacgcc agcccgagcg ggcgaaccag ttctcgaccc tctccgatct
     2101 tccggtcaat cgcctctacg ggcccgacga cctcaaaggt ttcgatctcg agcgcagcct
     2161 ggggcacccc ggtgaatatc cattcacgcg cgggattcat ccaacaatgt accgcggccg
     2221 gctgtggacg atgcgtcagt tcgcgggatt cgggtcggct gaggacagca accgccggtt
     2281 ccactatttg ctcgagaaag ggcagacggg tctctccgtg gccttcgaca tgcccacact
     2341 gatgggatac gactccgatc acccgcgcgc gctcggagag gtcggccgcg agggcgttgc
     2401 cgtcgactcg gtccaggata tggagctcct ctttcgcgat cttccgctcg accgcgtgac
     2461 cacctcgatg acgatcaacg cgccggccaa catcctcctg gccatgtacg tcgccgcggc
     2521 agaaaacatg ggcttcactc gcgcgcagat cggcggcacg acacagacgg atatgctcaa
     2581 agaattcatc gcccagaagg aatggatcgt gccgccgcgg cccagcatga agctgatcca
     2641 ggacatgctc gtcttcggca cccgagaact cccgcggtgg aacgtaatca gcatcagcgg
     2701 gtatcacatc cgcgaggccg gcgctacggc ggtgcaagag ctggcattca ccctggcgga
     2761 cggcattgcg tatgttcagg ccgggatcga ggcgggtctg gacgttgatg agtttgcgcc
     2821 caggctctcg ttcttcttcg actgtcataa cgatttcttc gaggaggtcg cgaagttccg
     2881 cgcggcgcgc cgcctctggg cccggatcat gcgcgaccgg ttccaggcca agaaccctcg
     2941 ctcgtggctg ctgcgcttcc atactcagac ggccggggtc tcgctcacgg cgcagcagcc
     3001 gctcaacaac atcgtccgcg tggcggttca ggcgctcgcg gcggtactcg gcggtacgca
     3061 gtcgctgcac acaaactcga tggacgaaac gtacgccctg ccgaccgagg acgccgtcat
     3121 ggtggcgctg cgcacccagc agatcatcgc ccacgagagc ggtgtcgcgg ataccgtcga
     3181 tccgttgggg ggatcgtatt ttgtcgaatc gttgacaacc accatggaag agcgggccga
     3241 ggcgtacata cagaagatcg atgatctcgg cgggatggtg agagcggtcg agctcggcta
     3301 tccgcagcgg gagatcgccg aggccagcta tcactatcaa cagcagctcg agcggaaaga
     3361 aaagatcatc gtcggcgtca acgagtttgt gcagcccgaa gagcagcgaa ttccgatcct
     3421 gcgcatcgac cccgaagtcg agcgcaatca gatcgagcgt gtccgtcgag tccgggctgg
     3481 gcgggacgag gtcaaagcgc gtcgcgcgct cgacgaggtc aagcgggccg ctgattcggg
     3541 cgagaatacg atgtataagg tcctggagtg cgtaaaggtc gggggaacca tgggagaaat
     3601 ttgtgatgtg ttccg
//
LOCUS       DBNGSB010000008         5287 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3075189, whole genome shotgun sequence.
ACCESSION   DBNGSB010000008 DBNGSB010000000
VERSION     DBNGSB010000008.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5287)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3075189"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="AC159B_00235"
     CDS             complement(<1..978)
                     /locus_tag="AC159B_00235"
                     /inference="COORDINATES: protein motif:HMM:NF014476.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HIR3376213.1"
                     /translation="MALFLREDDIALVLRMPEVIAAVEAVFREQGLGKVVNRPRQRVS
                     SEGTILHVMSAAVPALGVMGLKAYSSARGGARFLAMLYSTETGELLAVMEANRLGQIR
                     TGAASAVATKYLARPDAGSIGIIGTGWQARSQVVAVASVRPIALVKSFGRDERRRTAF
                     ADELIHELGGEVAPVDSAEEAVEGVDIVITATNARSPVLFGSWLQPGMHVNAIGSNWG
                     DRRELDTEAVGRAQRIVVDDLEQAKGESGDLIVAVEEGTIDWTRVVELGPIVAGTIPG
                     RTSESEITLFESQGIALEDVAAMKLAYDRAKEMGVGEPFKRNGGIMGEGD"
     gene            1031..1792
                     /locus_tag="AC159B_00240"
     CDS             1031..1792
                     /locus_tag="AC159B_00240"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3376214.1"
                     /translation="MDQKKSLVKAQFGRRAGAYARSESHARDRDLDLLLEHLQLHASD
                     RVLDVATGTGFTAVAMRHRVASVTGLDLTPAMLREAQRLAPALQIHWVEGDVEALPFC
                     HNSFSVVTCRRAPHHFVHLDQAIGEMLRVLQPGGRIGVIDQVNPEEAEGTRLMNELEM
                     LRDSSHVHALSISEWRTAFSRHKISLAFLDVVESTLSFERWMDLADTELDRRRTIEQT
                     LRTATPEARSLIGFSESPEPSFLKRWLVLVGQKSR"
     gene            complement(1822..2700)
                     /locus_tag="AC159B_00245"
     CDS             complement(1822..2700)
                     /locus_tag="AC159B_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376215.1"
                     /translation="MNLRLALSRLESAEARAGVARALAILCAFGYIFSLAVVFATGIG
                     LERWLFLLLVWVLLVYLPLRILLEAFQTIAPAIRRALVLQAASDPARYRSGGSIELIV
                     DGLYGQDVLMPRIAKPQDGQKARDASAAVLRMANRRSHTDVEHASHQCLGTVEQWTAD
                     LSAWAQSAAPQNIQARWAGLRALASFAALNKVLLGAVADQSGRRIVVPQPWRDPGEYL
                     DACLDFCDRLALEVDVMPWTEPPLGISVNPDDAAAIRTAWTAYVETPPPAIEARNNFV
                     KTLLDTTTGQQDNETT"
     gene            2737..3297
                     /locus_tag="AC159B_00250"
     CDS             2737..3297
                     /locus_tag="AC159B_00250"
                     /inference="COORDINATES: protein motif:HMM:NF016176.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HIR3376216.1"
                     /translation="MFVSLVWLLVAAASTFLPVGTFEIDRGASRIDFLMKDNRGGFTG
                     FVRDLTASVVVKEQGASFTGDVQVQIDARTITTGVSLRDNQMRREFLETEKFPFITFR
                     GTIAPVDRVGALPFRGTMKGTLAIKQVTQEIQIPVRITALADSYLADGQVTIKMSDFK
                     IPIPRFLIFVAEDPITISFKVRFLQK"
     gene            3319..4680
                     /locus_tag="AC159B_00255"
     CDS             3319..4680
                     /locus_tag="AC159B_00255"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HIR3376217.1"
                     /translation="MSALSYAQEHGSRFLDELKSFVSIPSISTLPQHKADVQRAADWL
                     LDHLRGMDMTGELIPLDGGHPIVYAEWRKAPGKPTLLIYGHYDVQPVDPLHEWTSPPF
                     EPTVRGDNLYARGACDDKGQVFAVIKALECLLRSDGALPVNVKVIVEGEEEIGSPGIY
                     KWIDRHGRKLACDFAWVSDGGLFAPGLPMITTGLRGLVYTEVDVRGPNHDLHSGQHGG
                     AAPNPVNAVASIIAGLKDRRGRVTVPRFYASVREPMPEEKASWERLPFNEEAYLKELG
                     ITVAPGEQGFGILERRWARPTLDVHGIVGGWTGEGSKTVIPARVVAKISMRLVPDQRA
                     VTIYKSFVKKVKKLASEGVEVEVRELASADPVLIDPKAKPVQVAATVVRQVWGADPVF
                     TREGGSVPIVAKFDRVLKVPTVLFGFGLPDDNLHAPNEKFHIPNFYKGIETVIRFLQQ
                     IES"
     gene            4786..>5287
                     /locus_tag="AC159B_00260"
     CDS             4786..>5287
                     /locus_tag="AC159B_00260"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR3376218.1"
                     /translation="MLSIKDTELEMVQLVLPEHANVRGTLYGGRMMDWITTSATMAAM
                     RLAKGAVLLASMDDLDFLHPVFISDVVTLRARVEFVGTSSMEVGVEVFAEDPERGNRR
                     LTTSSHLAMVAVDERASPRPVGTTLQPADGREREIYAEAQARKQLREAHLTDRREQAA
                     KIMDEVI"
BASE COUNT         1041 a         1606 c         1630 g         1010 t
ORIGIN      
        1 atccccttct cccattatcc cgccgtttct cttaaacggt tcgccgacac ccatctcctt
       61 tgctcggtca taagcgagct tcatggcagc cacatcttcg agcgcaatgc cctgcgactc
      121 gaacagcgta atttcgcttt cggatgtgcg gccggggatt gttccggcga cgatcgggcc
      181 gagctcgacc acgcgcgtcc aatcaatcgt tccttcctca acggcgacta tgagatcgcc
      241 cgattcgccc ttcgcctgct cgaggtcatc cacgacaatt cgctgcgccc ggccgaccgc
      301 ttccgtatcg agctcacgtc tgtcgcccca gttggacccg atcgcgttca cgtgcatacc
      361 cggctgtaac caagacccga acagcaccgg ggaacgtgcg ttcgttgccg tgatgacaat
      421 atcaactcct tcgaccgctt cctcagcgct gtcaacggga gccacctcgc cgcccagctc
      481 atggatcaat tcgtcggcga acgccgtccg gcggcgctcg tctctcccga aggatttaac
      541 gagtgcaatc gggcgcacgc tggcgaccgc aacgacttgg ctgcgtgcct gccatcccgt
      601 gccgatgatc ccgatggatc cggcatcagg cctggcgaga tacttcgtcg cgacggcgct
      661 tgccgcgccc gtgcggatct gccccagacg attggcttcc atgacggcaa ggagttcgcc
      721 ggtttcggtg ctgtagagca tcgcgaggaa gcgtgcgcct ccgcgcgcgc tgctgtaggc
      781 cttcagtccc atcacgccga gggccggaac agccgccgac atcacgtgaa gaatggtgcc
      841 ttccgaggat acgcgctggc gggggcggtt cacaactttg ccgaggccct gctcgcggaa
      901 tactgcctcg accgccgcga tgacctcagg catgcgcagg accagtgcga tatcatcttc
      961 tcgaagaaag agggccatgg cgcacacaaa cttctcgacg gcacgaacgc gtcctttaaa
     1021 ctgggaccca atggatcaga agaagagctt agtgaaggca caattcggac gccgcgcagg
     1081 tgcgtatgcg cgcagcgaaa gtcacgcccg ggatcgcgat cttgacctgc tgctcgagca
     1141 cctgcagctg catgcgtcag accgcgtgct tgatgtggcg acaggcacgg gattcacggc
     1201 cgtcgccatg cgtcaccgag tcgcgtccgt cacggggctc gatctcaccc ccgccatgct
     1261 gcgcgaagct caacgcctgg cgccggcatt gcagatccac tgggtcgagg gcgacgtgga
     1321 ggcgttgccg ttctgccata attcgttttc ggtagtgacg tgccgccggg cgccgcacca
     1381 cttcgtacac ctcgaccagg cgattggcga aatgctgcga gtgctgcaac ccggcggacg
     1441 gatcggcgtg atcgaccagg tcaaccctga agaagcggag gggacgcggc tgatgaatga
     1501 gctggagatg ctaagggatt ccagccacgt gcacgctcta tcgatcagcg aatggcggac
     1561 agccttctcc cgccacaaaa tctcccttgc gtttctggat gtcgtcgaat caacgctatc
     1621 tttcgagcgc tggatggatc tggcagatac cgaattggac cggcgcagga ccatcgaaca
     1681 aacgctgcga accgcgacac ccgaggcgcg gagcctcatc ggattctcgg agtcgccgga
     1741 gccgtcgttt cttaagcgat ggctcgttct ggtagggcaa aaatcgcgat gacgcgatga
     1801 cgcgatgacg cgatgacgcg attacgttgt ctcgttgtcc tgttgtcccg tcgtcgtgtc
     1861 cagtagagtt ttgacgaagt tattgcgtgc ctcgatcgcg ggcggcggcg tctcgacgta
     1921 cgccgtccac gccgttcgga tcgcggcggc atcgtcggga ttcacggata tgcccagggg
     1981 cggctcagtc cacggcatca cgtccacctc gagggccagc cggtcacaaa aatccaggca
     2041 tgcgtcgaga tattcgcccg gatcacgcca gggctgcggc accacaatcc ggcggccgga
     2101 ctgatcggcg acggcgccaa gaaggacctt gttgagcgct gcaaacgacg ccagtgcccg
     2161 aagccccgcc catctcgcct ggatgttctg gggggccgcg ctctgagccc acgccgagag
     2221 gtccgcggtc cattgctcaa ccgttccgag acactgatgg gacgcatgct ccacgtctgt
     2281 gtgcgacctc ctgtttgcca tcctgagcac cgccgccgat gcgtcgcggg ccttttgacc
     2341 atcctgaggc ttggcaatgc gcggcatgag cacatcctgt ccgtacaatc cgtcaacgat
     2401 cagttcgata gaacctccgc tccgataacg cgcaggatcg gaagctgcct ggaggacgag
     2461 cgcccgccgg atggccggtg caatcgtttg aaacgcttcg agcagaatgc gaagtgggag
     2521 gtagaccagg agcacccata ccagcaacag gaagagccag cgctcgagac cgatccccgt
     2581 ggcgaacact accgccaggg agaaaatgta cccgaaggcg cagaggatgg ccagcgcgcg
     2641 cgctacgcct gcgcgggctt cggcgctttc gagccggctg agggccaggc gaaggttcat
     2701 aggtagtccg ggagctcctg aggaagaagg tatcacatgt ttgtctcact cgtgtggttg
     2761 cttgtcgcgg ctgcctccac atttctgccg gttggcacgt ttgagattga tcgaggagcg
     2821 agccgcatcg atttcctcat gaaggacaac cgaggaggat ttaccggttt tgtgcgtgac
     2881 cttaccgcgt cggtcgtcgt aaaagagcag ggcgccagtt tcacgggcga cgtccaggtc
     2941 cagatcgatg cgcggacgat caccacgggc gtcagtctgc gggacaacca gatgcgccgt
     3001 gaattcctcg agaccgaaaa gtttccgttc atcacattcc gcggcacgat tgcccccgtg
     3061 gaccgggtcg gcgcgctgcc atttcgcggc accatgaagg ggacgctggc gatcaagcag
     3121 gtcacgcagg agatccagat tccggtgcgc atcacggcgc tggccgattc ctatctggcg
     3181 gacgggcagg tcacaatcaa gatgtccgac ttcaagattc ctattccacg ctttctgatc
     3241 ttcgttgcag aggatccgat cacgatctct ttcaaggtcc gctttctaca aaagtgaatg
     3301 gtgagtggga ggtttagtgt gagcgctctc tcgtatgcgc aggagcatgg gtcgcggttc
     3361 ctcgatgaat tgaaatcctt tgttagcatc cccagcatca gcacacttcc tcaacacaag
     3421 gcagacgtcc agcgggccgc ggactggctg ctggaccatc tccgaggtat ggacatgacc
     3481 ggcgagctga ttcccctcga tggcggccac cccatcgtct atgcggagtg gagaaaggca
     3541 ccaggaaagc ctaccctcct tatctatggt cactacgatg tgcagcctgt cgatccgctg
     3601 catgagtgga cgtcgccgcc gttcgagccg acggtgcgcg gcgacaatct ttacgctcgg
     3661 ggggcgtgcg acgacaaggg acaggtgttt gccgtcatca aggcgctgga atgcctcctc
     3721 cgctccgacg gagcgcttcc ggtgaacgtg aaggtgatcg tcgaagggga agaagaaatc
     3781 ggcagtcccg gcatctacaa gtggatcgac cgtcacggac gaaagctggc gtgcgacttc
     3841 gcctgggtat cagacggagg actattcgcc cccggcctcc cgatgattac cacaggtcta
     3901 cgcggtctcg tatacaccga ggtcgacgta cgcgggccca atcacgacct ccactcgggt
     3961 cagcatggcg gcgccgcacc gaacccggtc aatgcggtcg cgtcgattat tgccgggttg
     4021 aaggaccggc gcgggcgtgt caccgtgccg cgtttctacg cctctgttcg tgagccgatg
     4081 cccgaagaga aggcatcctg ggagcgactg ccgttcaacg aggaagccta cctcaaggag
     4141 ctagggatca ccgtggcgcc gggggagcag ggcttcggca tcctggagcg gcggtgggcg
     4201 cgcccgacgc tcgatgtcca cggtatagta ggcggctgga caggcgaagg atccaaaacg
     4261 gttatccctg cgcgcgtcgt cgcaaagatc agcatgcgtc tagtgccgga ccagcgggca
     4321 gtgacgatct acaaatcatt cgtgaagaaa gtcaagaagc ttgcgtcgga gggagttgag
     4381 gtggaagtgc gagaactggc gagcgctgat cctgtcctta tcgacccaaa ggcgaaaccc
     4441 gtacaggtcg ccgcgacggt cgtacgccag gtttggggcg cggacccggt gtttacgcgt
     4501 gaaggtggaa gcgtcccgat tgtcgccaag ttcgaccgcg tcctgaaagt ccccacggtg
     4561 cttttcgggt tcggtctgcc cgacgacaac ctgcacgcgc caaacgagaa gttccacatt
     4621 ccgaatttct acaaagggat cgagacggtg atcaggttcc tccagcagat tgaaagttga
     4681 ctccgggacc ggggaccggg accggggacc cggcaatcgg ctatggagtg acttagctac
     4741 gggtcccctc atcccggtcc ccgatcccag agtctaaatc ctccgatgct atcgattaaa
     4801 gatactgagc tcgagatggt ccagttggtg ctccccgagc acgccaacgt gcgcggcacg
     4861 ctttacggcg gacggatgat ggactggatt accacatcgg cgacgatggc cgctatgcgg
     4921 ctcgccaaag gtgcagtgct tctcgccagc atggatgatc tcgatttcct gcaccccgtc
     4981 ttcatcagcg atgttgtcac acttcgggcc cgtgtcgagt tcgtaggcac gtcctcgatg
     5041 gaggtcgggg tcgaggtctt cgcggaggat cccgagcggg gaaatcggcg gctcacgacc
     5101 tcatcgcatt tggccatggt ggccgtagat gaacgcgcct cgccgcgacc agtaggtacg
     5161 acgcttcagc cagctgatgg cagggagcga gagatctacg cggaagcgca ggcgcgtaag
     5221 cagctgcgag aggcacatct cactgatcgt cgagaacagg cggcaaagat catggacgaa
     5281 gttatcg
//
LOCUS       DBNGSB010000009         6135 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_774782, whole genome shotgun sequence.
ACCESSION   DBNGSB010000009 DBNGSB010000000
VERSION     DBNGSB010000009.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 6135)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6135)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6135
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_774782"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="AC159B_00265"
     CDS             <1..703
                     /locus_tag="AC159B_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007681662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR3376219.1"
                     /translation="SYPYMWSWGGTEIEKDGKTVNLNKKEVIESVKFMTAFWKEAHDE
                     GGLAWDDTNNNRAFLSQTICATLNGASIYIESLRNPDKYITEKGAQLKTDIQHAPLPK
                     GPAGQFAMHTFFSHVIPNYSKNQAGAKEFLKWIHTKANYEPWFVSQKGFATPPTREWE
                     KHKLWNEDKVMAPFKVAGLLGQTPGYPLPSSGKKAAEVLTKYIITDMYAKAVQGGSAE
                     DAVKWAQGELTKVYT"
     gene            889..1818
                     /locus_tag="AC159B_00270"
     CDS             889..1818
                     /locus_tag="AC159B_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011649003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3376220.1"
                     /translation="MAIAETQVYMPQAPRPRLRLLEDERWLAFVLLVPTLVLLGMFIA
                     YPFVRGILLSVTNARVGVPGDFVGLANFYKIFNDGIFRTSVYNTFVYTGVTTVFKLAL
                     GLWLAMLLNRNFRGKAFTRAFILLPFIIPTVLSTFAWKWMFDPTFSVLNWLLYHLGLI
                     TGRINWLGDPDLAMISIIIVNIWRGVPFFAISLLAGLQTISPELNEAAAIDGAKPWQR
                     FWHVTWPLLLPVTMVVVLFSVIQTFADFQIVYVMTGGGPANATHLFATYAYQIGIGTG
                     LLSEGAAIALAMFPILFLVVIIQLLYIRRVEVR"
     gene            1822..2673
                     /locus_tag="AC159B_00275"
     CDS             1822..2673
                     /locus_tag="AC159B_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3376221.1"
                     /translation="MIEGAIWRKWVFFYVPMALFLLFLLFPFYWMVITSVRPDGELYR
                     PWNSINYNPFWTWKPTLDHVRYLLEETLFASWLWNTTFIALVSTAISLVCGVFAGYAL
                     SRLNFPFAGSLGTGIFITYLVPQTLLFIPLAEMIRNYRLGDTPWALILTYPTFLIPFC
                     TWLMMGYFKAVPKELEECARIDGASRWQAMLYIVIPVAIPGILSAGIFAFTLSWNEFI
                     YALVFLSSPEQKTVPVGVTSELIRGDVFFWGPLMAGALLGSIPVAIAYSFFVEHYVAG
                     LTGSVKG"
     gene            2750..3841
                     /locus_tag="AC159B_00280"
     CDS             2750..3841
                     /locus_tag="AC159B_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3376222.1"
                     /translation="MGQVVLKGINKFFDSVHAVKDVNMQIRDNEIVVFVGPSGCGKTT
                     TLRMIAGLEAISSGEISIDGNVVNELAPMDRDIAMVFQNYALYPHMSVYDNMAFGLKM
                     RKFEKPEIDKRVREAADILGIGELLMRKPRQLSGGQRQRVALGRAIVRHPRVFLFDEP
                     LSNLDAKLRVQMRVELKKLHLRLGTTAIYVTHDQVEAMTLGDRVVVMKDGVVQQVGEP
                     LELYNQPANKFVAGFIGSPAMNFAAVTVTDANGSLIAENSGLRLKLPDETAQRLRGHI
                     GHEVTLGVRPEDLTVAGDADLDHPCFDAVIEVVEQLGSEILLDMKVGEGVMVASVEPT
                     VRVKVRDKLRFAMRPSRLHVFDAKTEAAI"
     gene            4036..4785
                     /locus_tag="AC159B_00285"
     CDS             4036..4785
                     /locus_tag="AC159B_00285"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR3376223.1"
                     /translation="MLFYRGMTCENVTIKGDKGTPVTAYVAKPSGPGPFPGVALVHHL
                     PGWSEFYIETTRRFAHHGYLAICANLYERAGEGNPDDVAAKVRADGGISDAQMVGDTE
                     AAVKWIRAQSAHNGKVGIFGSCSGGRHAFIYACQRKDVDACVELWGGRVVMNKEELNS
                     KTPVAPVEMTKDLSCPLLGIFGNDDRAPSPEQVNQHEAELKKYGKIHAFYRYDGAGHG
                     IFYWHRPLYRPEQAMDGWSKVFAFFSKHLAK"
     gene            4797..5114
                     /locus_tag="AC159B_00290"
     CDS             4797..5114
                     /locus_tag="AC159B_00290"
                     /inference="COORDINATES: protein motif:HMM:NF039404.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6295 family protein"
                     /protein_id="HIR3376224.1"
                     /translation="MCTSIIEIARAEGMAKRGDEWFPLSQAVVAYDHARHAPLGDVIT
                     LDFINTGLEPGARAGIELTLETAKELRAALDRAIAAAEFEEAEVRGKDAVPRLVRAAS
                     HAR"
     gene            complement(5783..>6135)
                     /locus_tag="AC159B_00295"
     CDS             complement(5783..>6135)
                     /locus_tag="AC159B_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HIR3376225.1"
                     /translation="LGDCVDYPLVIADGSTVIRPYLDSAFAKASLEPLPIIETNAIEI
                     MRHAAILDNGITFLTPFDIEFERRVGRLVYVPVRELAHETQTLMLIGHERGTSAIASV
                     LAEMLKAMMREAAA"
BASE COUNT         1191 a         1879 c         1849 g         1216 t
ORIGIN      
        1 cagctacccc tacatgtggt cgtggggcgg caccgagatt gagaaggacg gcaagactgt
       61 caacctcaac aagaaagagg tcatcgaatc ggtcaaattc atgacggcct tctggaaaga
      121 agcccatgac gagggcggcc tggcctggga cgacaccaac aataaccgcg cgttcttgtc
      181 gcaaacgatc tgcgccacac tcaacggcgc ttcgatctac atcgagtcac tgcgcaaccc
      241 cgacaagtac atcactgaga aaggcgcgca gctgaaaacc gacatccagc acgcaccgtt
      301 acctaagggc cctgccgggc aattcgccat gcacaccttc ttctcgcacg tcattccgaa
      361 ctactcgaag aatcaagccg gcgcgaagga gtttctgaag tggatccaca caaaggcgaa
      421 ctacgagccg tggttcgtct cccagaaggg cttcgcaacg ccgccgaccc gcgagtggga
      481 gaagcacaaa ttgtggaacg aggacaaggt catggcgccc ttcaaggtcg ccggcctcct
      541 cggccagacg cccggctatc ctctgccctc atcgggcaaa aaggcggccg aagtgctcac
      601 caaatacatc atcaccgaca tgtacgccaa agcagtccaa ggcggctcgg cggaggatgc
      661 cgtgaagtgg gcgcaaggtg aactcacgaa ggtctatact tgaaagtacg ctctcacctg
      721 gtttgtgcgg ctgcgaatgg ctgccacatg cagggcgcat cgggcttgag cggtcgagcc
      781 gggaggcggc gcgctcaggg gctgatccac cccgtcaccc gcgtagtgtc gggacaatcc
      841 caagggttcg catgacgcgc cagctggcgc gcatcgaggt gaagcgaaat ggcgatcgct
      901 gaaacccaag tttacatgcc acaggcgccg cggcccaggc tccggctgct cgaggacgag
      961 cgttggctcg cattcgtgct gctagtgccg accctggtgc tgctcggtat gttcatcgcc
     1021 tatccgttcg tgcgcggcat cctgctgtcg gtaaccaatg cgcgcgtggg tgtgcccggc
     1081 gacttcgtcg gcctcgccaa cttctacaag atcttcaatg acgggatctt tcgcacctcg
     1141 gtctacaaca ccttcgtcta tacgggtgtg accaccgtct tcaagctggc gctcgggttg
     1201 tggctggcca tgctgctcaa ccgtaatttc cgcggtaagg ctttcacccg tgctttcatc
     1261 ctcctgccct tcatcatccc gaccgtgctt tcgactttcg cctggaagtg gatgttcgac
     1321 ccgacattta gcgttctcaa ttggctcctc tatcatctcg gcttgatcac cggtcggatc
     1381 aattggctgg gcgatccaga cctcgccatg atctcgatca tcatcgtcaa catctggcgc
     1441 ggggtgccgt tcttcgccat tagcctgctc gccggcctgc agactatcag tcccgaactc
     1501 aacgaggccg ccgccatcga cggcgccaag ccgtggcagc ggttctggca cgtcacctgg
     1561 ccgctgctgc tgcccgtgac catggtcgtg gtgttgttct cggtgattca gacgttcgcc
     1621 gacttccaga tcgtctatgt gatgacaggc ggcggaccag ccaacgcaac gcacctgttc
     1681 gccacctacg cctatcagat cggcatcggc accggccttt tgagcgaggg cgccgccatc
     1741 gcgctcgcca tgtttccgat cctgtttcta gtcgtcatca tccaacttct ctacatccgc
     1801 cgcgtggagg tacgctgagc catgatcgaa ggtgcaatct ggcgaaagtg ggtgttcttc
     1861 tacgtcccga tggcgctgtt cctgcttttc ctgctgttcc cattctactg gatggtgatc
     1921 acttcggtgc ggccggatgg cgagctctac cgaccctgga attcgatcaa ctacaatccg
     1981 ttctggacct ggaaaccaac attggaccac gtcagatatt tgctggagga gacgctgttt
     2041 gcatcgtggc tgtggaacac gacgttcatc gcactcgtat caaccgctat ctcgctcgtg
     2101 tgtggcgtgt tcgccggcta tgctctgtcg cggctgaact ttccctttgc cggcagcctg
     2161 ggcaccggca tcttcatcac ctatctggta ccccagacgc tgcttttcat tccgctcgcc
     2221 gagatgatcc gcaactatcg gctgggtgat acgccctggg cactgattct gacctatccg
     2281 acctttctta ttccgttctg cacctggctc atgatgggct atttcaaggc agtgcccaag
     2341 gagctcgagg aatgcgcgcg cattgacggc gcctcgcgct ggcaggccat gctctacata
     2401 gtcatcccgg tggcgatccc gggcatcctc tcggccggca tcttcgcttt cacgctgtcg
     2461 tggaatgagt tcatctatgc acttgtcttc ctgtcctcgc ctgagcagaa gaccgtgccg
     2521 gtcggcgtca catcggagct gatccgcggc gatgtattct tctggggacc gctgatggct
     2581 ggcgccctgc tcggatcgat cccggttgca atcgcctatt cgtttttcgt cgagcactac
     2641 gtcgcaggat tgacaggatc ggtgaagggc tgacagcacc ctggataatc ggggttggaa
     2701 agcgcgcatg ctgaatgcgc acaggcggtc gggaggcagg tgggagcgaa tgggacaggt
     2761 cgtcctcaag gggattaaca agttctttga cagcgtgcac gctgtcaaag acgtcaatat
     2821 gcagatccgc gacaatgaga tcgtggtgtt cgtcggaccg tccggctgcg gcaagacgac
     2881 gacgctcagg atgatcgccg ggcttgaagc gatcagctcc ggcgaaatct caatcgacgg
     2941 caacgtggtc aatgaactgg cgcccatgga ccgcgacata gccatggtgt tccagaacta
     3001 cgcgctctac ccccacatga gcgtgtacga caacatggcg ttcggcctga aaatgcgcaa
     3061 attcgaaaag cccgagattg acaagcgcgt gcgggaggcg gcggacatcc tcggcatcgg
     3121 cgaattgcta atgcgcaagc cgcgccagct ctccggcggc caacgccagc gcgtggcgtt
     3181 aggccgtgcc atcgtgcgcc acccccgcgt gttcctgttc gacgagccgc tgtcgaacct
     3241 cgatgccaag ctgcgggtgc agatgcgcgt tgagctcaag aagctgcatc tgcgtctcgg
     3301 caccaccgcc atttacgtta cccacgatca ggtcgaggcc atgacgctgg gtgaccgggt
     3361 tgtcgtcatg aaggacggcg tggtgcagca ggtgggggag ccgctcgagc tgtacaatca
     3421 gcccgccaac aagttcgtcg ccggcttcat cggctcccca gccatgaatt tcgccgccgt
     3481 tacagtgacc gacgcgaatg gatcgctgat cgccgagaac tccggcttgc gccttaagct
     3541 gccggacgag actgcgcagc gtctgcgcgg ccatatcggc cacgaggtca cgctcggcgt
     3601 gcgcccggag gacctcacgg tggcgggtga tgccgatctc gaccacccat gcttcgatgc
     3661 cgtgatcgag gtggtcgagc aactcggctc ggaaattctg ctcgatatga aggtcggcga
     3721 gggtgtcatg gtggcgagcg ttgagccgac cgtgcgggtg aaggtgcgcg acaagctgcg
     3781 ctttgccatg cgcccttcca ggcttcacgt tttcgacgcg aagaccgagg cggcaatcta
     3841 acgccggcgc aagggaacct ctttcctctg tgccaagcgg ctcggctggt tcttgttgca
     3901 ccggtatgcg attggcgaat actagccgcg ccccgacccg ggagcagatg gagcgcgacg
     3961 ccgcttcttg atgatggcgc ggcgctgcga tgcaccgacg gacggctaaa ggctgaagcc
     4021 gatggaggaa ggctgatgtt gttctatcga ggcatgacct gcgagaacgt gaccatcaaa
     4081 ggcgacaagg ggaccccggt taccgcctat gtggccaagc cgtctggtcc gggccccttt
     4141 ccgggcgtgg cgcttgtcca ccatcttcct ggctggagcg agttctacat tgagacgacg
     4201 cgtcggttcg cgcatcacgg ctatctcgcg atctgcgcta acctctatga gcgtgcgggc
     4261 gagggtaacc cggacgacgt cgccgccaaa gtgcgcgccg acggcggcat ttccgacgcc
     4321 cagatggtcg gcgataccga ggccgcagtg aagtggatac gcgcgcagtc cgctcacaac
     4381 ggcaaggtcg gcattttcgg ctcctgttcc ggtggccggc acgccttcat ctatgcctgc
     4441 cagaggaagg acgtcgatgc ctgcgtcgaa ctctggggcg gccgcgtggt gatgaacaag
     4501 gaggagctca attccaaaac gcccgtcgcg ccggtcgaga tgacgaagga ccttagctgc
     4561 ccgctgctcg gcatattcgg caacgacgac cgcgcgccga gccccgagca ggtgaaccag
     4621 cacgaggccg agctcaagaa gtacggtaag atacacgcgt tctaccgcta cgacggtgcc
     4681 ggccacggca tcttctattg gcaccggcca ctctaccggc ccgagcaagc aatggatggc
     4741 tggagcaagg tgttcgcctt cttcagcaag catctggcga agtaggaggc ccggacatgt
     4801 gcacgtccat tatcgagatc gcacgtgcag agggcatggc taagcgcggg gacgaatggt
     4861 tcccgctctc tcaggcggtg gttgcctatg accacgcgcg ccatgcgccg ctgggcgacg
     4921 tcatcacgct cgacttcatc aacactggcc tcgaacccgg ggcacgggct gggattgagc
     4981 tgacgctgga gacagccaag gagctgcgcg ctgctctcga ccgggcgatt gccgccgccg
     5041 agttcgagga ggcggaagtg aggggcaagg acgccgtgcc caggctcgtt cgggcagcgt
     5101 cacacgcgag atgaggcggc cgcgaccgac ggcaagtcgt tacgaatagg tgctcactca
     5161 gtggaggtta tgggcctgac gagtggctgc ttaagagcgg gacccaacac aacttggcgg
     5221 acgtccgctt tgcgccagta gcagccattc cttaaacgct aattccacga attgaatcct
     5281 cggcaacgcc gcgaaacatc gggccaccgc tgacacctgg gtctggatac gtcgctttat
     5341 actaattctg ggcaaaacta tcataactcc ccgctcgcgc gacagcgatc cgcgtcagcc
     5401 agatagcgcc tgcgctgctc ctgtttgagt cagggttaga tcggtgaagc gcaaccactg
     5461 atacaggccg cccatcatgg gcaagtttga gctagctcta gcaaatcatc tccaatctgc
     5521 tacgggtgag cgcctacacg ctgcggctct gcaatcgtac tggatgagcg ctcgactgac
     5581 cggaaagccc gcacgccact gcaggggatt cgggtcagaa gtccgctgca tcgtgacacc
     5641 gttgaaagca tgcagatcat cgatcgaacg cggcgtgatt cttgaaatgt ttgccacgat
     5701 gaccgttccg gtcttcatat cgcatggagc gcgaagcggc tgcggccggc ccggcaatga
     5761 ctgcgggtga cgcgcgggtc gctcacgccg cggcctcacg catcatcgcc ttcagcattt
     5821 ccgcgagcac gctggcgatc gcgctcgtcc cgcgctcgtg gccgatcagc atgagcgtct
     5881 gcgtctcgtg cgccaactca cgcaccggca cgtagaccag gcggccgacg cgccgctcga
     5941 actcgatgtc gaacggcgta agaaaggtga tgccattatc aagaatggcg gcgtggcgca
     6001 taatctcaat ggcgttggtt tcgatgatcg gcagcggttc cagcgaagct ttcgcaaatg
     6061 ccgaatcgag gtagggccgg atcacggtcg acccgtcggc aatcaccagc ggatagtcga
     6121 cgcagtcgcc gagcc
//
LOCUS       DBNGSB010000010         7159 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3228684, whole genome shotgun sequence.
ACCESSION   DBNGSB010000010 DBNGSB010000000
VERSION     DBNGSB010000010.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 7159)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7159)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7159
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3228684"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..1641)
                     /locus_tag="AC159B_00300"
     CDS             complement(4..1641)
                     /locus_tag="AC159B_00300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR3376226.1"
                     /translation="MRGRRELIIVALLLTGAFILFAFGLGDSTLWDQDEARYTQIARE
                     ILATGDFITMHHNSAEWFVHPPLWMWLVALTGWLFGFSEFTARIWSPVFGVLGVYATF
                     LLGRLLFTVRAAILGSIVLMTTLQYFVQSRLAVFDTPLIAFMLLATYAFLRGVRENRR
                     DFILWAAAWTGLGTLTKGPIALILPSLALLAFFGLRREPIPWRWIPWQRAALIYSAIG
                     LTWYLVEWWRHGFAFISKVVGYYLFSRFFGVVELQSGPWWYYLPVFGIGAIPWTAFLA
                     VTIPYLARRRHDHEGSLFVLVWCAVTVGFYTIAGTKLPNYVLPVYPLAALGIGSLWDH
                     ALAGEPRARAYVRAGFVTTVLALAVGVWEVVTFARMQYPEQFASLQRHLVTLGVALIA
                     LFAVAVVFYLLRRPAAAFAGLAAVTVGMATVMIGQTLPLVEAHRPIKQVATIVVSSLL
                     PGEPLIAMGISDQQTLLYYTDRRLMWADSPFELLTAACYQPRALIVTRPGMVQKWNAH
                     LRLYGAVLEVETIAEVGDLLVARKNGDTPCRQPLVGR"
     gene            complement(1638..2717)
                     /locus_tag="AC159B_00305"
     CDS             complement(1638..2717)
                     /locus_tag="AC159B_00305"
                     /inference="COORDINATES: protein motif:HMM:NF015684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HIR3376227.1"
                     /translation="MAIGLLDQYLWRQLRGLFLFGVSIFTTLLMFNHLFLLARLVFQR
                     GAPIGTAIELLMYRLPYFLAFSFPMATLLAVLMAMNRLSDGQEITAMRTSGISLKRIA
                     VSIVAAGLLVSALTFGFNEGVVPPAEDRYRIAFAGLLEQPTPVEQQQVLFREQQEGVE
                     SVYYIRRFRTEAGRMEGVVVNQFEGGRLLRIIEAPDANYLDGAWIFLNGKMYLFTGST
                     TVTTTFERLRVGLRRTPKEIGIPQKEPSEMTIRELRAYVGVLRRSGENVARYSVELQS
                     KVAFPLSAMLFSLLAVPLGLRPHRTGTSIGFGLTIVVLLVYYLMISVTLTLGQGGRLH
                     PIAAAWLPNLVLAVGGILLLRRADR"
     gene            complement(2724..3440)
                     /gene="lptB"
                     /locus_tag="AC159B_00310"
     CDS             complement(2724..3440)
                     /gene="lptB"
                     /locus_tag="AC159B_00310"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HIR3376228.1"
                     /translation="MLVASGLIKRYGGRTVVNGVTLQVTRGEIVGMLGPNGAGKTTTF
                     YMMVGLVRPDGGEVLLDDVPLTHLPVHQRAKRGIGYLAQDPSVFRGLSVAQNILMVLQ
                     ANGTAPNERQARLDELLTEFGLTQVRDQLGITLSGGERRRVEIARVLAMNPAYVLLDE
                     PFTGVDPLAVSEIQDIVRYLKGRGIGIVVTDHNVRDTLAITDRATIINAGRILLSGTP
                     DEILVHPEVRRVFLGERFEL"
     gene            complement(3444..4283)
                     /locus_tag="AC159B_00315"
     CDS             complement(3444..4283)
                     /locus_tag="AC159B_00315"
                     /inference="COORDINATES: protein motif:HMM:NF015901.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HIR3376229.1"
                     /translation="MTTLKASSLPALFLMLTLLAPASAQPPRTPPIDLTGATYVEFDD
                     STGIWVLRGNPVTVTRGAATVRAMSMTYDSRQQIVRASGAVNFTDPSATLTSAQATFW
                     LQEERLLAEGGVSGSFRESGQETHLRSRTLEVWRKDRRAVASGDAEIRRAEFTLTSSR
                     VEYLEMQHKATATGRGTVTTPEGHLTADRIDVFLDREEGVAEGNVEISMRARGDQAAL
                     PIEGRAPKATLRRSDGIAVLSGGATVRLGQSTATAQEITVDLRRNRVVATGQAHLELY
                     PER"
     gene            complement(4280..4858)
                     /gene="lptC"
                     /locus_tag="AC159B_00320"
     CDS             complement(4280..4858)
                     /gene="lptC"
                     /locus_tag="AC159B_00320"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HIR3376230.1"
                     /translation="MNRGRTILIIGAAAAVILAAVIGLRRTAPPSTATAPPAQAQPAP
                     EPVEPDQPFLNIEGTQLSGADARGRRLWDLKATSLQVDRTKNVVTMNDVSGQLFENEK
                     PALAFRAPKATFFAASRDVQLSGGVFGRTPDGRTLRAPMVRWDHRNAIFIASGGVTVT
                     QPGMTIRADRLTSDAAMRHPKFSGNISVQVTQ"
     gene            complement(4855..6060)
                     /gene="lpxB"
                     /locus_tag="AC159B_00325"
     CDS             complement(4855..6060)
                     /gene="lpxB"
                     /locus_tag="AC159B_00325"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HIR3376231.1"
                     /translation="MPSTSNLQPSTIFVVAGEVSGDISGAHLVRELLRRNPSLRVTGV
                     GGPYLAAAGTTLFLDSSMWGTIGWLRAGQFMLVSKLLQIEREIRRVNPDLLIVVDFAA
                     FNLRLAERLGSLVPIVYYFPPMVSFRRGDRARKVARLGMRLLAAFRPEAAAYQAAGAD
                     VVFVGHPAVDLTKPRWTPRQARAHFSIPEDAPVIALLPGSRVAEISTHLPVMIRAAEM
                     LWRKSPELHFLVPLASERFRSLVTSRISSTKVSTKIPIRVVTEPYDAMAVSEVIVTAT
                     GTATLEAAVLAVPMVAVYRLPLLSWLIAHYALTIRRAALPNILSGRDIVPELLQDRMT
                     PEAIAVEVRKIREDPDRRRQMQADLRDVASDLGAPGVVQRAADEVFAVLTGDGLPLHK
                     RAGYDEQES"
     gene            complement(6060..6890)
                     /locus_tag="AC159B_00330"
     CDS             complement(6060..6890)
                     /locus_tag="AC159B_00330"
                     /inference="COORDINATES: protein motif:HMM:NF017989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxI family protein"
                     /protein_id="HIR3376232.1"
                     /translation="MKHQDSSDVIGLIAGDGRLPVVLAAAVKSRGLRLVCIMINGEQG
                     ELVRLADHAYRVGFGELHRILEAFGTHGVRRVLLAGRIPRASLIAGGDRAFRQWMEGA
                     SDRSDHGLFQQGVERLAGLGIEVASPLEFVHDLLTPRGVLTKRSPTDEEWDDARTGMR
                     IAKKIAGLDIGQTLVLKRGVMLAVEAAEGTDQTIRRAGTITDGAIVVKASRPHQDERF
                     DLPTAGAQTVHALADARAAVLALEAERTLLVDRAEALAAADAAGIAIVGLEPALNVEG
                     "
BASE COUNT         1428 a         2332 c         2184 g         1215 t
ORIGIN      
        1 tcctcacctt cctacgagcg gctgccggca gggcgtgtcg ccgttctttc gcgcgaccag
       61 cagatcgccg acctcggcga tggtctcaac ctcgaggacc gcgccgtaca gtcgcaaatg
      121 cgcgttccac ttctgcacca tcccgggtcg cgtcacgatc agcgcgcgag gctggtaaca
      181 ggccgccgtc agcaactcga atgggctgtc cgcccacatc agcctgcggt ccgtgtaata
      241 cagcaacgtc tgttggtcgg aaatccccat ggcgatcagc ggctcgccgg gcagcaggga
      301 ggacacgaca atcgtggcca cctgtttgat cggccggtgc gcctcgacca gcgggagcgt
      361 ctggccaatc ataacggtcg ccattcctac agtgacggcg gcaaggccgg caaacgccgc
      421 cgcgggccgt cggagaagat agaagacgac ggcgacggca aacagcgcga tcagggcgac
      481 ccccagtgtc accagatgcc gctgcagcga tgcgaactgc tcgggatact gcatccgggc
      541 aaaggtgacg acctcccaga ctcccacggc gagggccagc acggtcgtga caaatccggc
      601 gcgcacatac gctctggcgc gcggttcgcc ggcaagcgcg tggtcccaga gtgatccgat
      661 gccgagggcg gcgagagggt ataccggaag aacgtagttg ggaagcttgg tacccgcgat
      721 cgtgtagaat ccgacggtca cggcgcacca gacgagcacg aaaagactcc cctcatgatc
      781 gtgccggcgc ctggcaaggt acggaatggt cacggccaga aacgcggtcc agggaatcgc
      841 tccgatcccg aacaccggca ggtaatacca ccacggcccg gactgcagct cgaccactcc
      901 gaagaaccgg ctgaagagat aatatccaac caccttcgag atgaacgcga agccatgacg
      961 ccaccattca accagatacc aggtcaatcc gatcgccgaa tagatcaaag ccgcccgctg
     1021 ccacgggatc caccgccacg gaatcggctc ccttcgcagc ccaaagaatg ccagcagcgc
     1081 aagtgagggc aggatcaggg cgatcggccc cttggtcaga gtgccgagtc ctgtccacgc
     1141 cgcagcccac aggatgaagt cgcgcctgtt ctctcgcacc ccccgcaaga acgcgtacgt
     1201 ggcgaggagc atgaatgcga tcaagggggt gtcaaaaacc gcgagacgcg actggacgaa
     1261 atattgcagg gtggtcatca acacgatcga gccaaggatc gcagcgcgca ccgtgaacag
     1321 caggcgcccg aggagaaagg tcgcatatac gccgaggacg ccgaagaccg gcgaccagat
     1381 ccgagcggtg aattcggaga atccaaagag ccatccggtc agcgccacca gccacatcca
     1441 caggggggga tgcacgaacc actcagcgct attgtggtgc atggtgatga agtcgccggt
     1501 cgcgagaatc tccctggcga tctgtgtata gcgggcctcg tcctgatccc acagggtgct
     1561 gtcgccgagg ccaaacgcga acaggatgaa cgcaccagtg agcagtagcg caacgatgat
     1621 taattcacgc cggcccctca tctgtcggca cggcggagga gtaggatgcc ccccacggcc
     1681 agcaccagat tgggtagcca ggccgcagcg atcggatgca gccgcccacc ctgccccaac
     1741 gtcagcgtca ctgagatcat gaggtaatac accagaagga cgacgattgt gagcccaaac
     1801 ccgatcgagg taccggttcg gtgggggcgg aggccaagcg gtacggccag cagcgagaag
     1861 agcattgcgg agagcgggaa tgcgactttg gactgcagct ccaccgagta tcgcgcgacg
     1921 ttctctccac tcctccgaag caccccgaca tacgcgcgga gctcgcggat ggtcatctct
     1981 gatggttcct tctgcggaat accgatttct ttcggcgtgc gccgcagtcc cactcgcagc
     2041 cgctcgaacg tcgtcgtcac cgtcgtcgat cccgtgaaca gatacatctt tccgttcaga
     2101 aaaatccacg cgccatcgag gtaattggca tccggcgcct caatgatgcg cagcagccgc
     2161 ccgccctcga actggttcac gacaacgcct tccatgcggc cggcttcggt ccggaagcgg
     2221 cggatatagt agacggattc cactccttcc tgttgctcgc gaaagaggac ctgctgctgc
     2281 tcgacgggtg ttggctgctc taacaatccc gcgaacgcga ttcgataccg gtcctcggcc
     2341 gggggcacga cgccctcgtt gaacccaaac gtgagggcgc tcaccagtaa tccggccgcc
     2401 acgatcgaga cggcgatgcg tttcaagctg atgccgctgg tgcgcatcgc cgtgatctcc
     2461 tggccgtccg agagacggtt catcgccatc aacaccgcta gcagcgtggc catagggaaa
     2521 ctgaacgcca ggaaatacgg cagccggtac atcagcaact cgatggctgt accgatcggc
     2581 gcgccacgct ggaacacgag acgcgcaagc aggaagaggt gattgaacat cagcagcgtg
     2641 gtgaagatag agaccccaaa caagaaaaga ccgcggagtt gacgccacaa atactggtct
     2701 aagagtccga tcgccatcgt cagttagagt tcgaaccgct ctcccaagaa cacacgtctc
     2761 acctccgggt ggacgaggat ttcgtcggga gtcccggaga ggaggatgcg tcctgcgttg
     2821 atgatggtgg cgcggtcggt gatcgccagc gtatcgcgaa cgttgtggtc ggttacgaca
     2881 atcccaattc cgcgaccctt gaggtaccgc acaatgtctt gaatctccga gaccgccagc
     2941 gggtcgacgc ccgtaaacgg ctcatccagc agcacatacg ccggattcat tgccaggacg
     3001 cgcgcgatct ccaccctccg ccgctcgccg cccgacaatg tgatgcccag ctggtcgcgc
     3061 acttgggtga gtccaaattc cgtcagaagt tcgtcgaggc gtgcctggcg ttcgttgggt
     3121 gcggtcccgt tcgcctgaag caccatcaag atgttctgtg cgacgctgag tccacgaaac
     3181 acggacggat cctgcgcaag atagccgatc ccgcgcttgg cccgctggtg caccggcaga
     3241 tgcgtgagcg ggacgtcatc taggagcacc tcgccgccgt cgggccgcac cagacccacc
     3301 atcatgtaga aggtcgtcgt cttgccggcg ccgttggggc ccagcatccc gacgatctcg
     3361 cccctcgtca cctgcagtgt gacaccattg acgacggtgc gcccgccata ccgcttgatc
     3421 agaccgctgg caacaagcac gtgctaacgc tccggataca gctcgagatg cgcctgtccg
     3481 gtcgcgacta cgcggttgcg ccgcaggtcg acggtgattt cctgggccgt cgccgtactc
     3541 tgccctagac ggaccgtcgc accaccagac agaacagcga tgccgtcgga tctcctcagc
     3601 gtcgccttag gcgcacggcc ctcgattggc agggccgcct ggtctccacg agcgcgcatc
     3661 gagatctcga cattgccctc ggcaacgcct tcctctcgat cgaggaagac atcgatgcga
     3721 tcggcggtga ggtgaccctc cggggtcgtc accgtgcctc ggcctgtggc ggtcgcctta
     3781 tgctgcatct caagatactc cacgcggctg ctggtcagcg tgaattcagc gcgccggatc
     3841 tcggcgtctc ccgaagcaac ggcccggcga tcctttctcc agacctcgag agtgcggctc
     3901 cgcagatgcg tctcctgccc gctctcacgg aacgacccgg agacgcctcc ctcggcgagc
     3961 agtcgctcct cttgcaacca gaacgttgcc tgtgcagagg tgagcgttgc ggacgggtcg
     4021 gtgaagttca ccgctccgga cgcgcggacg atctgctggc ggctgtcgta cgtcatagac
     4081 atggcacgaa cggtggcggc cccccgcgtc accgtaaccg gattgcctct caatacccag
     4141 atgcccgtgg agtcgtcaaa ctcaacatac gtcgcgccgg tcaggtcgat cggcggcgtg
     4201 cgcggcggct gcgcagaggc cggcgcgaga agcgtcaaca tgaggaagag agcgggaagg
     4261 gaagaggcct tcagtgtcgt cactgtgtca cctgaacgga gatgttccca ctgaacttcg
     4321 ggtgccgcat cgccgcatca gacgtcaggc ggtcggcgcg aatggtcatc cccggctgcg
     4381 tcacggtcac gcccccggat gcgataaaga tggcgttccg atgatcccat cgcaccatcg
     4441 gcgcccgaag cgtgcggccg tcgggagttc gcccgaatac ccctcccgaa agctggacat
     4501 ctctggaggc ggcaaagaac gtcgcctttg gcgccctgaa cgccagggcc ggtttctcgt
     4561 tctcgaacag ctgtccgctg acgtcgttca tggtgacaac gttctttgtc cgatccacct
     4621 ggagggacgt tgccttgaga tcccacaggc ggcgcccacg ggcatcggcg cctgagagct
     4681 gtgtgccctc gatgttgagg aagggttggt ccggttccac cggctcgggt gccggctgcg
     4741 cctgcgcggg cggcgctgtc gccgtcgagg gtggcgccgt acgacgcagg ccgatcactg
     4801 cggccaggat caccgccgcg gcagctccta ttattaggat cgtgcgccca cggttcatga
     4861 ctcttgctca tcgtatcctg ccctcttatg cagcggcaac ccgtctccgg tcagcacggc
     4921 aaacacctcg tcggccgcgc gctgcaccac tccgggcgcg ccgagatccg aggcaacgtc
     4981 tcgcagatcc gcctgcatct gtctccggcg atccggatcc tcgcggatct tgcgcacctc
     5041 gaccgcgatc gcttcgggcg tcatcctgtc ctgcagcagc tcgggtacaa tgtcacgccc
     5101 cgagagaatg ttgggcagcg cggcacgacg gatcgtcaag gcatagtgcg caatcagcca
     5161 gctgagcaac ggtagccggt agactgcgac catcggcacg gcgagcacgg ccgcctccag
     5221 cgtggcagtc cccgtcgccg tcacgatgac ttcggaaacc gccatcgcgt cgtacggctc
     5281 ggtcacaaca cgaatgggaa ttttggtgga gactttggta gacgagatgc ggctggtgac
     5341 gagtgaccgg aaccgttcag aggccagcgg gacgagaaag tgaagctcag gcgactttcg
     5401 ccacagcatc tcggccgcgc gtatcatcac cggaaggtgc gtgctgattt ccgcaacccg
     5461 gcttccggga aggagagcga tcaccggagc atcttctgga atactgaagt gcgcacgggc
     5521 ctgccgcggc gtccagcgcg gcttcgtcaa atcaaccgcg gggtggccca caaacacgac
     5581 gtctgcgccg gccgcctgat acgccgcggc ctcgggacgg aacgccgcca gcaatcgcat
     5641 acccaggcgt gcaaccttcc gcgcccggtc gccccggcgg aacgatacca ttggtggaaa
     5701 gtagtacacg atcgggacca gactgcccaa acgttccgcg aggcgaagat tgaacgcggc
     5761 aaaatcgacg acgatcagca aatcagggtt gacacggcgg atctcccgct cgatttgcaa
     5821 cagcttcgaa acgagcatga actgacccgc tctgagccat ccgatcgtgc cccacatcga
     5881 actgtcgagg aacaacgtgg tcccggcagc ggcaaggtat ggcccgccca cgcccgtgac
     5941 cctcaaggat gggtttcggc ggagtagctc gcggacgagg tgggccccgg agatatctcc
     6001 ggagacttcg ccagccacca caaagattgt tgaaggttga aggttggagg ttgaaggcat
     6061 caaccttcaa cattcaacgc aggttcgagt cccacgatgg cgattcccgc ggcatccgcg
     6121 gcggcgagcg cttccgccct gtccacgagc agtgtgcgct ccgcctcgag cgcgagtaca
     6181 gccgccctcg catcagccag agcatgaacg gtttgagcgc cagcggtggg gagatcaaat
     6241 cgctcgtcct ggtgcggccg tgaggccttg accacgatgg cgccatcggt gatcgttccg
     6301 gcgcgccgaa tcgtctgatc cgtgccctcc gcggcttcga ccgccagcat gacgccccgt
     6361 ttcagaacga gcgtctggcc gatgtcgagc cctgcgatct ttttcgcgat tcgcattccg
     6421 gttcgcgcgt catcccattc ttcgtctgtg ggggagcgct tcgtcagaac acctcgtggt
     6481 gtaagaaggt cgtgaacgaa ctcgagcggg ctcgcgacct cgatgccgag tccggcgaga
     6541 cgctcgacgc cctgctggaa cagaccgtgg tcgctgcggt cggacgcgcc ctccatccac
     6601 tgccggaatg cccggtcgcc accggcgatg agactcgcgc gcggaatccg tccggcgagc
     6661 aggactcggc gcacaccgtg cgtgccgaag gcctcgagaa tgcggtgaag ctcgccgaat
     6721 cccactcgat atgcgtgatc ggcgagccgg acgagttcgc cctgttcgcc gttgatcata
     6781 atgcagacca gtcggagccc gcgcgacttc accgccgcgg cgagcacgac cggcagcctt
     6841 ccatcccccg cgataagacc gattacgtct gatgaatctt ggtgtttcaa cgcagaccac
     6901 gcgtccatcc gcgagaccgg gtgacctttg gacaaaggac ccgtagctaa gtcactccac
     6961 cgccgattgc cgggtcccgg ttcgaggcct cccggtctcg cgatcacttc actccttagc
     7021 cgattgccgg gtcccggttc gaggcctccc gcgaagagcc ctattacttc tgatgcatca
     7081 ctctggtcca actcagacaa cgctcttcat cacgagaccg ggtgaccttt ggacaaagga
     7141 cccgtagcta agtcactcc
//
LOCUS       DBNGSB010000011         3318 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_622091, whole genome shotgun sequence.
ACCESSION   DBNGSB010000011 DBNGSB010000000
VERSION     DBNGSB010000011.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_622091"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..1465
                     /gene="icd"
                     /locus_tag="AC159B_00335"
     CDS             218..1465
                     /gene="icd"
                     /locus_tag="AC159B_00335"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NADP(+))"
                     /protein_id="HIR3376233.1"
                     /translation="MIALETLTPPKEGQKIEIHDGRLSVPNQPIIPFIEGDGTGPDIW
                     RASQRIFDAAVRLAYGGKRRVVWFEVYAGEKANKAYGSWLPEDTLRAFQHYVIGIKGP
                     LTTPVGGGFRSLNVALRQMLDLYACVRPVRWFEGVPSPVKHPEWVDVVIFRENSEDIY
                     VGLEHPSLSPEAKKLIEFVRKEFGWKVRDDAGVGLKPMSPFGTKRLVRKALRYAIDHR
                     RTSLTIVHKGNIMKYTEGAFREWGYQLARDEFGTQTITWDDVQKQHGGKVPAGKILIQ
                     DYIADVTFQHLLTRPKDFDVIATGNLNGDYLSDAVAAQVGGIGMAPGGNISDHHAVFE
                     ATHGTAPKYAGLDKVNPGSLTLSGVMMLEHLGWNEAAEMIIKGLEKTFKQKIVTYDLA
                     RLMDGAREVKTSEFATAVIDNMA"
     gene            1489..2418
                     /gene="mdh"
                     /locus_tag="AC159B_00340"
     CDS             1489..2418
                     /gene="mdh"
                     /locus_tag="AC159B_00340"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HIR3376234.1"
                     /translation="MGRPKVTIVGAGMVGHTTAHWLAVDQVADLVLVDIVDKMPSGKA
                     LDLLQAAPIRGIDLKIVGSNGYPETGGSDVVVIIAGIARKPGMTREQLIDTNAGIVKG
                     VVEQVTRHSPNAYLVVVTNPLDAMTYLTYKISGFPRQRVIGESGALDSTRFRTFIAAE
                     VGVSVEDVHAMVIGAHTDKDMVPLPRFANVGGVPLSQLLSGERIEAIVARTRRGGAEI
                     TELIGTSAFFAPGAAIAQMVEAIVRDRKRLIPCSVFLEGEYGQKDVCIGVPIVLGGGG
                     LERIIEVPLDEQERSAFSASAGAVREMLGGLKL"
     gene            2425..>3318
                     /locus_tag="AC159B_00345"
     CDS             2425..>3318
                     /locus_tag="AC159B_00345"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020031.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042709 - succinate-CoA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004774 - succinate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit beta"
                     /protein_id="HIR3376235.1"
                     /translation="MKLHEFQAKELFAKHAIPIQKGTVIERPEQLDGLTLNYPLVLKA
                     QVLVGGRGKAGGIKVANTLSEARQYGRAILSMDIKGEKVRRLLVAEAADIGAEFYLAF
                     VVDRGVRGLVAIASAAGGIDIEDVARQTPEKIAKVAIDPFLGFQPFHGRGLGRNIGLT
                     GTLLDDFVKISTALHRLCVGEDAELAEINPLAVVRDRLLAVDAKVIIDDNAVFRHPDL
                     PKNEELTELEQMARASGLSYVELDGDIAIIGNGAGLVMATLDIVAHFGGHPANFLDVG
                     GGATEENMRRAVDIVARKPGVR"
BASE COUNT          667 a          973 c         1090 g          588 t
ORIGIN      
        1 ctcgatcagg ggtcgccata tcggtgattt acccagctgg cctgctggtc agaccagctt
       61 ggactccatc ctaccggtcg cggcggtcag tgtcaaggat gcacgagcct ccgggcgaaa
      121 ccctgaagag gcatgcagat ttcgccaacc acagcgcgtc gttttgtcat cgcaggagca
      181 tgctacactg gtcgaggcca attaggagga catcttcatg atcgcgctgg agacccttac
      241 cccaccaaaa gaggggcaga agattgagat ccatgatggt cggctgtcgg tacccaacca
      301 gccgatcatt ccttttatag agggggatgg gacggggccc gatatctggc gcgccagcca
      361 gaggatcttt gacgctgcgg tccgcctcgc gtacgggggg aagcgccggg tcgtgtggtt
      421 cgaggtctac gctggggaga aggcaaacaa ggcatatggc tcgtggctgc ccgaagacac
      481 cctccgcgcg ttccagcact acgtcatcgg gattaaagga cctctgacga cgccggtggg
      541 cggtgggttc cgcagtctca acgtcgccct gcggcagatg ctcgatctct acgcctgcgt
      601 gcgtccggtg cgctggttcg aaggggtacc cagtccggtc aagcatcccg agtgggtcga
      661 cgtcgtcatc ttccgcgaga actccgaaga catctacgtc gggctcgagc atcccagcct
      721 ctctccggaa gcaaagaaac tcatcgagtt cgtgcgcaag gagttcggct ggaaagttcg
      781 agatgacgcc ggggtcgggc tgaaaccgat gagcccgttt ggcacgaagc ggctggtgcg
      841 caaagccttg cgctacgcga tcgaccaccg ccgaactagc ctgacgatcg tgcacaaagg
      901 caacatcatg aagtacaccg agggcgcgtt tcgggagtgg ggctatcagc tggcccgcga
      961 cgagttcggc actcagacga ttacctggga cgacgtgcaa aagcagcatg gtggaaaggt
     1021 gccggccggc aagatcctga ttcaggatta catcgccgat gtgacgttcc agcatttgct
     1081 gacgcgcccg aaggacttcg atgtcatcgc caccggcaac ctcaatggcg actacctgtc
     1141 ggacgccgtc gccgcccagg tcggcggcat cgggatggcg ccgggcggca acatcagcga
     1201 tcaccacgcg gttttcgagg ctacgcacgg cacggctccc aagtacgcgg gactcgacaa
     1261 ggtcaatccc ggctcgctga ccctctccgg tgtgatgatg ctcgaacatc ttggctggaa
     1321 cgaggcggcg gagatgatca tcaagggatt ggagaagacc ttcaagcaga aaatcgtgac
     1381 ctatgatctc gcgcgcctca tggacggcgc cagggaagtg aagacgagcg aattcgctac
     1441 cgccgttatc gataatatgg cctgagacgc cgtctggggt gcacgataat cggcagacca
     1501 aaggtcacaa ttgtcggagc cggaatggtc ggccatacca ccgcgcattg gctggccgtg
     1561 gatcaggtcg ccgatcttgt cctggtagac atcgtcgaca agatgccctc ggggaaggcg
     1621 ctcgacctgc tccaggccgc gccgatccgc ggcatcgacc tgaagatcgt tgggagcaac
     1681 gggtacccgg aaacgggcgg ctccgacgtc gtggtcatca tcgccggcat cgcgcgcaag
     1741 cccgggatga cccgcgagca gctcatcgat acgaacgcgg ggatcgtaaa gggcgtcgtg
     1801 gagcaggtga cccgccattc tcccaatgcc tacttggtcg tcgtcaccaa ccccctcgac
     1861 gccatgacct acctgaccta caagatctca ggctttccac ggcagcgcgt catcggcgaa
     1921 tccggggccc tcgacagcac acgctttcgg acattcatcg ccgcggaggt cggagtctcc
     1981 gtggaagacg tgcacgcgat ggtcatcggc gcgcacacgg acaaagacat ggttccgctg
     2041 ccccgctttg cgaacgtggg gggcgttcca ctcagccagt tgctcagtgg ggagcgtatc
     2101 gaggcgatcg tggcccggac tcgtcggggc ggcgccgaga tcacagagct gatcgggacc
     2161 agcgcattct tcgcgccggg cgcggccatc gctcagatgg tagaggcgat cgtgcgtgat
     2221 cgaaaacgat tgatcccatg cagcgtcttc ctcgaagggg agtacggaca gaaggacgtg
     2281 tgcatcggcg tgcccatcgt gcttggcggc ggaggtctcg agcggatcat tgaggtccct
     2341 ttggacgagc aggagcgaag cgcgttcagt gcatcggccg gcgccgtgcg cgagatgctg
     2401 ggagggctga aactgtaacg agtaatgaaa cttcacgaat tccaggccaa ggaactcttc
     2461 gcgaagcacg ccatccccat tcaaaaagga accgtgatcg agcgtccgga gcagctggac
     2521 ggattgacgc tcaactatcc gctcgtgctc aaggcccagg ttctcgtcgg cggccgcggc
     2581 aaagccggcg gcatcaaggt ggccaatact ctctcggagg cacggcagta tgggcgggcc
     2641 atcctgagca tggacatcaa aggcgagaaa gtccggcggc tgctggttgc tgaggccgcg
     2701 gacatcggcg cggagttcta ccttgcgttc gtcgtcgatc gcggcgtgcg ggggctggtc
     2761 gcgatcgcct cagcggcggg gggcatcgat atcgaggacg tggcgcgaca gacaccggaa
     2821 aagatcgcga aggtggcgat cgatccgttt cttgggttcc agccgtttca cgggcgaggg
     2881 ctggggagga acatcgggct gaccggcacg ctgctcgatg atttcgtgaa aatctcgacg
     2941 gcgctgcacc gcctatgcgt cggcgaggac gcggaactgg ctgagatcaa tccgctggcc
     3001 gtcgtgcgag atcgtctgct ggccgtcgac gcgaaggtga tcatcgatga taacgcagta
     3061 ttccggcatc cggacctgcc caagaacgag gaacttaccg agctcgagca gatggcgcgc
     3121 gcgtcagggc tctcgtacgt ggagctcgac ggcgacatcg cgatcatcgg caacggcgcg
     3181 ggattggtga tggcgaccct cgacatcgtc gcccacttcg ggggccatcc ggcgaacttc
     3241 ctcgacgtgg gcggtggggc caccgaagaa aacatgcggc gggccgttga catcgtggcg
     3301 cgaaagcctg gtgtccgt
//
LOCUS       DBNGSB010000012         2731 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_163398, whole genome shotgun sequence.
ACCESSION   DBNGSB010000012 DBNGSB010000000
VERSION     DBNGSB010000012.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_163398"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="AC159B_00350"
     CDS             <1..508
                     /locus_tag="AC159B_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376236.1"
                     /translation="TTGASATFPLFIKMDYRKATGAVTVRGTTAPGTVVQVPGATAVV
                     KQSGTYALKTSLPVNLVAVKGTQIRRFRFDLPPKAKPHLSYLLVNADLSKMRSSVTGQ
                     LTMTEHPAAKVFVEHVEKGSKHQAAIIKGRFSLGGLPLVKGKNTLRWSLQLGFIHSPG
                     PDITFTTE"
     gene            complement(516..1904)
                     /locus_tag="AC159B_00355"
     CDS             complement(516..1904)
                     /locus_tag="AC159B_00355"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase family protein"
                     /protein_id="HIR3376237.1"
                     /translation="MNLIVLVLDSLRQDHVSFYHQGRGPFPDVPACRTPNLDAFAAQS
                     LVFNKAYPEALPTIPARYVLMTGQRALPFRPWAPLQPGELTIAQMLRSEGYVCGLITD
                     CYHYRAPGMNYHAGFNAYRWVRGQEYDPWESAPTRRQIDDYVNKNYPPEWRQRVAQFL
                     ANTDAFASEADWFPARVVDEAIGWLKKNRAHKQLFLWVDCFDPHEPWDPPSKFDTYSD
                     RGYRGPRLILPMGGLSRVWAAQEEVRQIRGLYAGEVAFVDHCLEPFFAALAELGYLDD
                     SVIVVLSDHGHPLSDHGKFLKGADRLYSELLHIPFMVRLPGGRHSGTRTDALVQFQDL
                     LPTLLDLVGLPSHADAMHGRSFADVLDRRAGEHRDAIITGYHEGADRCIRTHSWSYIE
                     RPTGEEDELYDLRSDPREQKNLIQDRPNEAKELARRFGRLYRRRARRRAGVQGKYELA
                     SSGLEEPVIGGD"
     gene            2015..>2731
                     /locus_tag="AC159B_00360"
     CDS             2015..>2731
                     /locus_tag="AC159B_00360"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR3376238.1"
                     /translation="MPPLPTGTVTFLFTDIEASTRLLGQLRDRYAEVLAEHRSLLRTA
                     FAETGGAEVDTQGDAFFVAFSRAKDAMLAAVVAQRALHRHAWPSGTAVRVRMGIHTGE
                     PLLTETGYVGMDVHRAARISQASHGAQILLSQTTRDLISADLPDGISLLDLGEHRLKD
                     MSEPQRLFQVVAPELPAEFPPIWSLGTIPNNLPRQLTSFVGRAHEVEEIERLLSQTYV
                     LTLAGAGGCGKTRLAAHVAGQ"
BASE COUNT          526 a          873 c          834 g          498 t
ORIGIN      
        1 gaccacgggg gcgtccgcca cgttcccctt gttcatcaaa atggactatc gcaaggccac
       61 gggtgccgtc accgtaagag gcacgaccgc cccgggcacg gtggtgcagg tcccgggtgc
      121 gaccgccgtt gtcaagcagt cgggcacgta tgccctcaag acgtcgctcc cggtgaacct
      181 ggtcgcggtg aagggcactc agatccgccg gttccgcttc gatctaccgc cgaaggcgaa
      241 gccccacctc tcgtaccttc tcgtgaacgc tgatctctcc aagatgcgca gctctgtcac
      301 gggacagctc acgatgaccg agcatccggc ggcaaaagta ttcgtggagc atgttgagaa
      361 ggggtcgaag catcaggccg cgatcatcaa aggcaggttc agcctcgggg ggttgcccct
      421 cgtgaagggc aaaaacacgc tgcgctggtc gctgcagctg ggatttattc acagcccggg
      481 gccggacatc acgttcacca ccgaatagtc agcgtctagt caccgccgat cacgggctcc
      541 tcgagcccgg acgaagcgag ttcgtatttc ccctgcaccc cggcgcgccg ccgggcgcgg
      601 cgtctataga gccgcccaaa gcggcgggcc aactctttcg cctcgttggg tcggtcctga
      661 atgaggtttt tctgttctcg cggatcactg cgaaggtcgt ataactcgtc ttcctcgccc
      721 gtgggccgct cgatgtagct ccatgagtgc gtccggatgc agcggtcggc tccttcgtga
      781 tagccggtaa taatggcatc gcgatgttcg ccggctcgac gatcgaggac atctgcgaaa
      841 ctccgaccgt gcatcgcgtc ggcatgagac ggcaagccca ccagatccag aagcgtggga
      901 agcaggtcct ggaactgcac gagcgcatcc gtgcgcgtcc cgctatgccg gccgcccggc
      961 agcctgacca tgaagggaat gtgcaataac tcgctgtaga gccggtctgc gcctttgagg
     1021 aacttgccgt ggtcagacag cgggtggccg tggtcggaga gcaccacgat gactgagtcg
     1081 tccaggtatc ccagttcggc gagggccgca aagaacggct ccaggcagtg atcgacaaac
     1141 gccacctcgc ccgcgtacag tccgcggatc tgacggactt cctcctgcgc tgcccacact
     1201 ctgctgagcc ctcccattgg cagaatcaag cgcggcccgc ggtagccgcg atcagagtag
     1261 gtatcgaatt tcgaaggtgg atcccagggc tcgtggggat cgaaacagtc aacccacaag
     1321 aacagttgct tatgagcgcg gttcttcttg agccagccga tcgcctcatc aacgacccgc
     1381 gccgggaacc aatccgcctc ggaagcaaac gcgtcggtgt tggcaaggaa ttgcgcgacc
     1441 cgttgccgcc attccggcgg atagttcttg ttcacgtaat cgtcgatctg acggcgggtg
     1501 ggcgccgact cccacggatc gtactcttgc ccccgcaccc atcggtacgc gttaaaaccg
     1561 gcgtggtagt tcattcctgg agcccggtaa tgataacaat cggtgatcag tccgcagaca
     1621 tagccttcgc tgcgcagcat ctgcgcaatc gtcagctctc ccggctgcag cggcgcccag
     1681 ggccggaacg gcagggcccg ctggccggtc atcagtacgt agcgcgccgg aatcgtcggg
     1741 agcgcttcgg gataggcttt gttgaacacc agagactgcg ccgcaaaggc atcaaggttc
     1801 ggcgtccggc aggcgggcac gtcgggaaac gggccacgac cttgatgata gaaactgacg
     1861 tgatcctgcc gcaggctatc caagacaaga acgatgaggt tcattttcgg gtctcctttc
     1921 cgtcgaacgc acgattctcg gcgcattccg cagggttcct ccagagagat catcgaaaga
     1981 gagcgccacc agcaaatctg gcgcgtgcag agccatgccc ccactgccga ctggaacggt
     2041 caccttcctc tttactgaca tcgaggcgtc cacacgactg ctcgggcagc ttcgcgaccg
     2101 ttatgcggag gtgctggccg aacaccggtc gctgctgcgc acagcctttg ctgagacagg
     2161 cggtgcagaa gtcgacacac agggcgacgc gttcttcgtg gccttctccc gcgccaaaga
     2221 cgcaatgctc gccgcggtcg tcgcgcaacg tgcattacat cgacacgcct ggccttcggg
     2281 aactgccgtg cgggtccgta tgggaatcca tactggggaa cctctcctga ccgaaacagg
     2341 ctatgtggga atggacgtgc atcgcgccgc gcggatctcc caggccagcc acggtgcgca
     2401 gattctcctc tcccagacca cgcgcgatct catcagcgct gacctgccgg acggcatctc
     2461 cctgttggat ctgggcgaac accggctcaa ggacatgagt gagccgcagc ggttgttcca
     2521 ggtcgtggcg ccggaactcc ctgccgagtt ccccccgatc tggtcgctag gaaccatccc
     2581 taacaatctt cctcggcagc tcacaagctt cgtgggacgg gcgcacgagg tcgaggagat
     2641 cgaacggctc ctatcacaga cgtacgtcct gacactggcc ggcgccggcg ggtgcggcaa
     2701 gacccggctg gctgcgcatg tcgcgggaca g
//
LOCUS       DBNGSB010000013         3796 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_1696987, whole genome shotgun sequence.
ACCESSION   DBNGSB010000013 DBNGSB010000000
VERSION     DBNGSB010000013.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_1696987"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="AC159B_00365"
     CDS             complement(<1..273)
                     /locus_tag="AC159B_00365"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR3376239.1"
                     /translation="MAEVSSHLDHLKKVPIFSDLPRKELEWIARSLKERVYEPGAVIV
                     KQGDPGVGFFLIAEGKVEVTHNSHKIREMGPGEFFGEMALMADRART"
     gene            393..1061
                     /locus_tag="AC159B_00370"
     CDS             393..1061
                     /locus_tag="AC159B_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376240.1"
                     /translation="MNRLVRAAPWLTLGAVFIALLSVNAFVVGVSGQGHQHSPGHSHA
                     PGAAAAATPDQVAAADKLLRDVKASLTHYIDVSAAEAGGYRQTTPYRFLRWGPAHFHN
                     YAYNRDDGVLDPRRPESLVYFKLPRGQVVLIGAMFLAPKGKGPRPGGSMTEWHVHNNL
                     CITATGSVALSNGQGRCPPGAFFIGDAVEMMHVWTFNHPDGPFAHEMTADAIKAALAE
                     YGGR"
     gene            complement(1122..1607)
                     /locus_tag="AC159B_00375"
     CDS             complement(1122..1607)
                     /locus_tag="AC159B_00375"
                     /inference="COORDINATES: protein motif:HMM:NF013921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR3376241.1"
                     /translation="MDRLYYLRRLAAAFKILNVQVLAYVLMSTHVHLVLQFTIPNLDV
                     VIHRVHQRYAIRFNQRYGRIGHTFNNRFQSRPIVDDIYLLQATRYVHLNPVEAGLVAH
                     PREYPWSSYRQYVAPGEGQLVNPEPVLTILGNDLLRCRVAYEKFVTTHLSRDRRRGSA
                     Y"
     gene            complement(1880..1955)
                     /locus_tag="AC159B_00380"
     tRNA            complement(1880..1955)
                     /locus_tag="AC159B_00380"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1920..1922),aa:Arg,seq:ccg)
     gene            2006..2335
                     /locus_tag="AC159B_00385"
     CDS             2006..2335
                     /locus_tag="AC159B_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376242.1"
                     /translation="MHRAKQGTRNAGYRQVTLLDRRGRVLAYADSLAVSDRTVGLARA
                     SFSPTGIVRLSMGRERVTVTSGGRVVRVLNGFRLTIHTGGQRSGDGSWTCEAAIGTIS
                     RSQGRSV"
     gene            2406..>3796
                     /locus_tag="AC159B_00390"
     CDS             2406..>3796
                     /locus_tag="AC159B_00390"
                     /inference="COORDINATES: protein motif:HMM:NF015736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HIR3376243.1"
                     /translation="MEEATEIERSLLSGFIRDRFLLGRAMEAGFRPELLPHNAGRMLA
                     RVLTDIYGDKEMPLEPVAVGAILRERGLMSAEMRRFVESVTEAPEPDAAQVMAYVDLL
                     KARTSRLRLVEVAKTISDYLESERNDGGDVVDFVGTLIPTLLEIQQQRLSRSSPPTHE
                     FGAKIVREITAGHSGSTLGYSLSPFDRLTEVVSGLRRGFYYGLAGAPRRGKTNFALEL
                     AASIAARQKIPVLYYSWEQTRRVLFARLLGREALVNPAALLSAEANAPTIQGRVKDAW
                     GKMEDGLQTLFLFEAGRQDTVERIKARAHNLMHTHQTDECVIFLDYLQRVPLEENVHD
                     EKARTDLISAKLADMSLELNCPIFAISPLDKEGCRLDEKPGDELSEFLRPTMHHCVGS
                     GDLEYDLDVAMVMVKDWVATKNLEELIKTEAKSGRTESDYLPRIDIIDLHLDKNRDAP
                     ESIASTIQYAFFVT"
BASE COUNT          761 a         1132 c         1156 g          747 t
ORIGIN      
        1 ggtgcgcgcg cgatctgcca tcaaggccat ctcgccgaaa aattctccgg ggcccatctc
       61 gcggatcttg tgactgttgt gggtgacctc gacctttccc tctgcgatga gaaagaagcc
      121 tacacccgga tcgccttgtt tgacgatcac ggcgccgggt tcgtacactc gctctttcag
      181 cgaccgggcg atccactcca gctccttccg gggaagatcg ctgaaaatgg ggaccttttt
      241 caaatgatcg agatgggatg agacttcggc cataggtagc agtctttctc cctggtctgc
      301 ggcttccctg ccggatcaac cccagcgggg gaacgcgcgt cttatacagt gaacggagca
      361 ctttgtatca accacatagg gagtgaacgg acgtgaacag gctcgttcgt gctgcgccgt
      421 ggctaacgct cggggcagtc ttcatcgctc tcttatcggt caacgctttt gttgtgggcg
      481 tctcgggtca aggtcaccaa cactcaccag gtcacagcca cgctccggga gcggctgccg
      541 ccgcgacgcc ggatcaggtg gctgcagcgg acaagctgct gcgagacgtc aaagcctcgc
      601 tcactcacta cattgatgtg agcgcggcgg aggcaggcgg ataccggcag accactcctt
      661 atcgcttcct gcggtgggga ccagcgcact tccacaatta cgcatacaac cgtgacgacg
      721 gcgtgctgga tcctcgccgg ccggaatcgc tggtgtactt caagcttccc agagggcagg
      781 tcgtgttgat tggcgcgatg ttcctcgccc cgaagggaaa ggggccgcgg cccggcggtt
      841 caatgaccga gtggcacgtg cacaacaatc tctgtatcac cgcgacgggc tcggtggcac
      901 tcagcaacgg ccagggtcgg tgcccaccgg gagcattctt catcggagat gcggtcgaga
      961 tgatgcacgt gtggaccttc aaccatcccg acggcccgtt cgcgcacgag atgactgcag
     1021 acgcaatcaa agcggctctc gctgagtacg gcggccggtg aactaacaga ccctccatct
     1081 gaggtgtact atgggagccc ggtgacggga tcccgtagta tttaatacgc gctccccctg
     1141 cgtctatcgc ggctaagatg tgtcgtaacg aatttttcat atgctaccct gcaccgaagt
     1201 aagtcattcc cgagaattgt gagcacgggt tccggattca cgagctggcc ctcgcccggt
     1261 gctacatatt ggcggtagct gctccaagga tattctcgtg gatgggcgac caatccagcc
     1321 tcaacggggt taaggtggac atatcgagtg gcctgcagca gatagatatc atcaacaatc
     1381 ggccgactct gaaaccgatt gttgaaggta tgcccgatcc ggccatagcg ctgattgaat
     1441 ctgattgcat agcgctgatg gacacggtgt attaccacat caaggttagg gatggtgaac
     1501 tgaagcacca ggtgcacatg cgttgacatc agaacgtacg ccaacacttg gacgttcagg
     1561 attttgaaag ccgctgcgag tcgccggagg taatatagac ggtccacgtc gtcaaggaag
     1621 atgacctgca aattgtttcc tcgcgcgaag acgtgataaa tgcctccgcc tactacaacg
     1681 cgcggactcc aaggcaagag gatgcctcca accggggtag ctgctacctt ttgtgctgca
     1741 gaagggtacc tgctaccgtt tttcagccaa attacaacat gcgggatcgg cggggtcgat
     1801 aacccaaatg ggctagtcgc gcgggcggag ttgtttcaga attgctccag ggaaacttga
     1861 cgcgtattgt ttcgaagagt ggcgcgcccg gggggatttg aacccacggc ctttggctcc
     1921 ggaggccaac gctctatccg ctgagctacg ggcgcgttgc cctgaacccc aacgtggttc
     1981 agggcgcagc ccgattatat caaccgtgca ccgagcgaaa caaggaacac gcaatgcggg
     2041 ctatcgtcaa gtcacgctgc tcgatcgtcg cgggcgggtc ctcgcctatg ccgatagcct
     2101 cgcggtgtcc gatcgcaccg tgggactggc gcgcgcgagt ttttctccga caggcatcgt
     2161 ccggttatcc atgggacgcg agcgggtgac ggtgacctcc ggggggcgcg tggtgcgcgt
     2221 gttgaacggc ttccgcctca ccatccacac cggcgggcag cgctcgggcg atggatcgtg
     2281 gacgtgtgag gcggccatcg gcacgatcag ccgctcacag gggcgcagtg tctagggcat
     2341 tccaggccgt ggcatatccc gagggcgaga tagaacgctc acgtcgggat cggcggtgcc
     2401 ctccattgga agaagctacc gagattgaac gctcgctact gagtggattc atccgcgacc
     2461 gttttctgct cgggcgggcg atggaggccg gctttcggcc ggagctcttg ccccacaacg
     2521 ccggccgcat gctggcccgc gtgcttaccg acatctacgg cgacaaagaa atgccgctcg
     2581 agccggtcgc agtcggcgcg atcctccgtg agcgcgggct catgtctgcc gagatgcgcc
     2641 ggttcgtcga atctgtcacg gaagctcccg agcccgacgc cgcgcaggtc atggcttacg
     2701 ttgatctcct caaggccaga accagccgcc tccgcctcgt tgaagttgcg aagaccattt
     2761 cagactacct ggaaagcgaa cggaatgatg ggggtgacgt cgtcgatttt gtcggcaccc
     2821 tcattcctac gctcctggag attcagcagc aacggctcag ccgcagctca ccccccaccc
     2881 atgagttcgg cgcgaagatt gtcagggaga tcacggccgg tcattcaggc tccacgttgg
     2941 gctattcgtt gtcgccgttc gaccgactca cggaagtagt ctcggggctg cggcgcggat
     3001 tctactacgg cctggcgggg gccccgcggc gtggcaaaac aaatttcgcc ctcgagctgg
     3061 ctgcatcgat cgcggcgcgc cagaagattc ccgtgctgta ctactcgtgg gagcagacgc
     3121 gccgcgtgct gttcgcgcgc ctgctaggac gcgaagccct ggtcaacccg gccgcgctgc
     3181 tgtccgccga ggcgaatgcg ccgacgatcc aggggcgcgt gaaggatgcg tggggcaaga
     3241 tggaggatgg tctgcaaacg ctcttcttgt tcgaggccgg ccggcaggat accgtcgagc
     3301 gtatcaaagc ccgcgcgcac aacctgatgc acacgcacca gaccgatgag tgcgtcatct
     3361 tcctcgatta tctgcagcgc gttcccctcg aggagaacgt gcacgacgag aaggcgcgca
     3421 cggatctcat ttccgccaaa ctcgccgaca tgagcctcga gctcaactgc cctatttttg
     3481 cgatttctcc gctcgacaaa gaaggatgcc ggctcgacga aaagccgggc gacgaactct
     3541 cagagttcct gcgccccacg atgcaccatt gcgtgggcag cggcgatctc gagtacgacc
     3601 tcgacgtcgc gatggtcatg gtcaaggatt gggtggcgac gaagaacctg gaagaactga
     3661 tcaagaccga ggccaagtcc ggtcggaccg aaagcgatta cctgccgcgc atcgacatca
     3721 tcgatctgca cctggacaag aaccgcgatg cgccggaatc gatcgcgtcg acgattcaat
     3781 acgcgttctt cgtgac
//
LOCUS       DBNGSB010000014         4708 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3537218, whole genome shotgun sequence.
ACCESSION   DBNGSB010000014 DBNGSB010000000
VERSION     DBNGSB010000014.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3537218"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..1022)
                     /locus_tag="AC159B_00395"
     CDS             complement(9..1022)
                     /locus_tag="AC159B_00395"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR3376244.1"
                     /translation="MMTVLRQHRWIFLMLVVVLVLPVPVRAQQGPLSPESVDAVDRAI
                     VLVHTYRESGGRTSRGSGSGIIIDSTGLILTADHVAARSTRLEVVLQNGEVYPATVVG
                     TDPLFDSALVRIQTNRSLPSAFLGASTGLAPGDSVMAFGRAPRRQSGPTGGTFLAFDV
                     DARPGVLNLRSSAVAWPGDSGGALVNGRGEVVGVIVAITRDGNISLSVSIDAIKHIMN
                     DLRLGFVRHPWLGLTGTTISEQIAQEHGLTVRQGVLVFEVFEGGPAAQAGLRAGRATG
                     AGNVPQGGDVIVAIDGRSTVTFGQMAAYILSKRIGDAIRLDMLRDGQPLGATVILAER
                     PSL"
     gene            complement(1102..2589)
                     /gene="zwf"
                     /locus_tag="AC159B_00400"
     CDS             complement(1102..2589)
                     /gene="zwf"
                     /locus_tag="AC159B_00400"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HIR3376245.1"
                     /translation="MAAIPDPDPATFVIFGVTGDLSQRLLLPALFRLYSERSIPEEFA
                     VVGVARKMLRSEEFRKFVKDAVTKAHGTVDEQAWQRFASRLYYTPGDFSGQGDHGPVA
                     LVVQRVEQQHRTGGNRVFYLATPPSTFEGILTTLHKMELVRPGRRNGAWNRVIIEKPF
                     GQDQASARELNTLVDRVIGEENIFRIDHYLGKETVQNMLVLRFANTVFEPIWDRRYVD
                     HVQITFVEEEGIGGRGRFYEEVGVVRDVVQNHVFQLLAIIAMEPPALFDAEGFRNEKA
                     KVLRAIPPIQPEDAVRGQYGPGEVRGNHVPGYRQEEGISGTSMTPTYAAMRLAIDNWR
                     WAGVPFYLRAGKRLHKKDTEVVVVFRQPPLRLFKDFDVSGMDHNVLVLNTTEEEKISL
                     SFAARVPGHQQQIRSVALDFDYARFGPAEHAPYEHLLLDVLRGMRRFFPRRDEVELQW
                     TIVDPLLEYWDANLPDDFPNYPAGSQGPAAAEAMLQKDGRHWRTG"
     gene            2649..2725
                     /locus_tag="AC159B_00405"
     tRNA            2649..2725
                     /locus_tag="AC159B_00405"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2683..2685,aa:Pro,seq:tgg)
     gene            complement(2876..3493)
                     /locus_tag="AC159B_00410"
     CDS             complement(2876..3493)
                     /locus_tag="AC159B_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376246.1"
                     /translation="MPPELKRFFTYWALTFAVLIFYTFAALVAGLDIWEHIMDNVRKR
                     RAQLKPKGPLLDPNPLKGKNVAATIGMGPGQQPNEKRQWVLDRATEVAKQLKLNVGIL
                     ALEDNKEHPLVQITDGKSLKAYRVDKTWVAEARAGNTQRESQIKDLLERYMSSDFLGN
                     EDVRPKRTTELAREAAAKPSAATRAAQPTQETPATESPETQEQQS"
     gene            3616..4446
                     /gene="ku"
                     /locus_tag="AC159B_00415"
     CDS             3616..4446
                     /gene="ku"
                     /locus_tag="AC159B_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end joining protein Ku"
                     /protein_id="HIR3376247.1"
                     /translation="MRPIWKGHLTFGLVTIPVKLYTATDSKDIRFRLLHKSCLTPIQN
                     KRYCSHHDQIVEWNDVVRGYEYEKGKFVSLTEEDLESVPLETAGTVSVTSFVTLDEIN
                     PLFYEKSYYLAPDEGGQKAFRLLHDTMSEATRVAVGKVVIKEKEHLVSVRPHDGALVL
                     STLYFADEVRAVDDIPELPVQTKIHPNEKKMAMQLIEGLSAEFAPTEYTDDYRQALEK
                     VINAKVQGEPIEAPARKPEKVVDLMEALRRSLQVTRKEKPARRSRPAARAAGMRERHR
                     "
     gene            4534..>4708
                     /locus_tag="AC159B_00420"
     CDS             4534..>4708
                     /locus_tag="AC159B_00420"
                     /inference="COORDINATES: protein motif:HMM:NF014017.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HIR3376248.1"
                     /translation="MARLYAGTSGFSYPEWKGKFYPAGLPASEMLRFYSRALATVEIN
                     NSFYRYPTEETLRQ"
BASE COUNT          922 a         1470 c         1389 g          927 t
ORIGIN      
        1 acgagacgtt agagggatgg cctttccgca aggatgaccg tggcgcccag cggctggccg
       61 tcacggagca tatcgagccg gatcgcgtcg ccgatccgct tggacaggat gtacgcggcc
      121 atctgcccga acgtcaccgt cgaccgtcca tcgatcgcga cgatcacgtc gccaccttgc
      181 ggaacgttac ccgcgccggt ggcgcgaccg gcacgtagtc ccgcctgagc tgccggaccg
      241 ccctcgaaca cctcgaagac gagcaccccc tgccgcacgg tcagcccgtg ctcctgggcg
      301 atctgttcag agatggtcgt cccggtcaag ccaagccagg gatggcgcac aaagcccaag
      361 cgcagatcat tcatgatgtg tttgattgcg tcgatcgaga ccgacaggct gatgtttccg
      421 tcgcgcgtga tggcgacaat cacgcccacg acctcgccgc ggccgttgac cagcgcaccg
      481 ccggaatcgc ccggccaggc aactgccgac gagcgcaggt tcaggacgcc ggggcgcgcg
      541 tcgacgtcaa acgccaggaa cgtcccaccg gtaggaccgg actggcggcg aggggcgcgg
      601 ccgaatgcca tgacgctgtc gccgggggcc agcccggtcg atgcgccaag gaacgcggaa
      661 ggcagcgagc ggttggtttg aatgcggacc agcgccgagt cgaataatgg atctgtaccg
      721 acgacggtcg ccggatacac ctcgccattc tgaagcacga cctcgagccg tgtggaacgc
      781 gccgcgacat ggtctgccgt cagaatcagc ccggtggagt caatgatgat ccccgatccg
      841 gacccgcgcg aggtccgtcc cccgctctcg cggtacgtat gcacgagaac gatcgcccgg
      901 tcgacggcat cgacactttc aggcgaaagt ggaccctgct gggctcgcac cgggaccgga
      961 agcacgagca cgaccaccaa catcaggaag atccagcggt gctgccgcaa taccgtcatc
     1021 atcgatcctc catccattcc cccgccgtct aacatttgag acagcctagc ggtcgattgt
     1081 gtttattcgg tcacagccca attatcccgt tctccaatgt cttccatcct tctgaagcat
     1141 agcctccgcc gccgccggtc cctgtgagcc tgcgggatag ttcggaaaat cgtcgggcag
     1201 atttgcgtcc cagtactcga gcagtggatc gacgattgtc cattgcaatt ccacttcgtc
     1261 gcgacgcggg aagaaccgcc gcatgccccg gagcacgtcc agcagcaggt gctcgtaggg
     1321 cgcatgctcg gctggtccaa accgcgcata atcgaaatcc agcgccacgg agcggatctg
     1381 ctgttggtgt cccggcacgc gtgccgcgaa gctcaacgag attttttctt cctcagtcgt
     1441 gttgagcacc agaacgttgt ggtccatccc gctgacatca aagtccttga acagtcgcag
     1501 cggcggctgg cggaacacga ccacgacctc ggtatctttc ttgtggagtc gcttcccggc
     1561 ccgaaggtag aacggcacgc cggcccagcg ccagttgtcg atcgccaggc gcattgcggc
     1621 gtaagtgggt gtcatcgagg tgccgctgat gccttcctcc tgccggtacc ccggtacatg
     1681 gttgccccga acttcgcccg ggccgtactg accccgcacg gcgtcctcgg gctggatcgg
     1741 ggggatcgcg cgcagcactt ttgctttttc atttcgaaac ccctccgcat cgaagagcgc
     1801 cggcggctcc attgcgatga tggccagaag ctgaaacacg tggttctgga ccacgtcgcg
     1861 cacgacgccg acttcctcgt agaatcgccc gcggccgccg atcccctctt cctcgacgaa
     1921 tgtgatctgc acgtggtcga cataccggcg gtcccagatc ggctcgaaga ccgtgttcgc
     1981 aaaccgcagc accagcatgt tctgcacggt ctccttgccg aggtagtggt cgatgcgaaa
     2041 gatgttctct tcgccgatca ctcggtcgac gagcgtattg agttctcgcg ccgaagcctg
     2101 gtcctgtccg aatggctttt cgatgatgac cctgttccag gcgccattgc gccggccggg
     2161 ccggaccagc tccatcttgt gcaatgtggt gaggatgcct tcgaaggtcg aaggaggagt
     2221 cgcaaggtaa aatacgcggt tgccaccggt gcggtgctgt tgctccaccc tctgcaccac
     2281 aagcgcgacc ggtccgtggt cgccctgccc gctgaaatcg cccggagtgt aatacagtct
     2341 cgaggcgaag cgttgccaag cctgctcgtc cactgtaccg tgagcctttg tgacggcgtc
     2401 cttgacgaac ttgcggaact cttcgctgcg gagcatctta cgagctacac ccaccaccgc
     2461 gaactcttcg ggaatggacc gctcactgta cagtcgaaac agcgccggca gcagcaaccg
     2521 ctgggacaga tcgccggtaa cgccaaaaat tacgaatgtc gcaggatctg gatcaggaat
     2581 tgccgccatc tgaccctctc actggaatgg agttccttcc catgatacga tgcccacaga
     2641 agtcccctcg gggcgtggcg cagcatggta gcgcgcgtgg tttgggacca cgaggtcgcc
     2701 ggttcaagtc cggccgcccc gaccatgaac cactcgcttc gcttgggttc atggcggcgc
     2761 catccctatc cccctccaca ttcttacgag tggttcatgc cctgaatccg aacggagtga
     2821 gtggttcagg gctattccga ctttcccaaa gacgactagg aaaaacaacg gagggttagg
     2881 actgctgttc ctgagtctct ggagattcag tcgcgggggt ctcctgcgta ggctgcgctg
     2941 ctcgagtggc ggctgaaggt ttcgccgccg cttcacgcgc gagttctgtg gtgcgcttag
     3001 ggcgcacatc ctcgttccct agaaagtcag acgacatata gcgctcgagc aggtctttga
     3061 tctggctttc tcgctgcgta ttccctgcgc gcgcttcggc aacccaggtt ttgtcgacac
     3121 gataggcttt gaggctcttc ccgtcggtga tctgcaccag ggggtgctcc ttgttgtctt
     3181 ctagggcgag gatcccgacg ttcagtttga gctgcttcgc aacctcggtc gctcgatcga
     3241 ggacccattg acgcttctca ttcggctgtt gaccggggcc cattccgatg gtcgccgcga
     3301 cgttcttccc tttcagagga ttcggatcca gcaatggccc cttgggtttg agctgcgcgc
     3361 ggcgcttgcg cacgttgtcc atgatatgtt cccagatgtc gagccctgcg accaatgccg
     3421 caaacgtata gaagatgagg acggcgaacg tcagcgccca gtaagtaaag aagcgcttca
     3481 actctggggg catggaccgc tcctctcccg cacccttttc atgtagtgga gatttggcgc
     3541 gcgtgcagcg gcctcctcca cgccagtatc agacggtata atagagatat ctgttaacgc
     3601 gcaagggaga cgacgatgcg tcctatttgg aaaggccact tgaccttcgg tctcgtaacc
     3661 atcccagtca aactgtacac ggccaccgac tcgaaagaca tccggttccg cctccttcat
     3721 aagtcctgcc tgacgccaat tcaaaacaag cggtattgct cacaccatga tcaaatcgtc
     3781 gagtggaacg acgtggtccg aggctacgag tatgagaaag ggaagttcgt ctccctcacg
     3841 gaggaagacc tagaaagcgt gccactcgag acggccggca ccgtcagcgt cacctcgttt
     3901 gtgacgctgg acgagatcaa cccgcttttc tacgagaagt cctactatct ggcacccgac
     3961 gaaggtgggc aaaaggcctt ccggctgctg cacgatacga tgagtgaagc cacccgggtt
     4021 gccgtcggta aggtggtgat caaggagaag gaacacctgg tctcggtgcg accgcatgat
     4081 ggtgcgctgg tgctcagtac gttgtacttt gccgacgagg tccgtgccgt cgacgacatt
     4141 ccggagcttc ccgtgcagac caagattcac ccgaacgaga agaaaatggc gatgcagtta
     4201 atcgaagggc tttccgccga gttcgccccc actgagtaca cggacgacta ccgccaggct
     4261 ctggagaaag tcatcaacgc caaggttcag ggtgagccga tcgaggctcc ggcccgcaaa
     4321 cccgagaaag ttgtcgacct catggaggct ctccgccgga gcctgcaggt cacgcgcaag
     4381 gagaaacccg ctcgacgttc gcgacctgcg gcacgcgctg cggggatgcg agagcggcat
     4441 aggtaaactg cggtcattgg tcgtgggtcg taggtcgtag gtaatgcagc cgtttcttgc
     4501 tacgacccac gacctacgac ctacgaccgc ttgatggcgc gtctctacgc cggcaccagt
     4561 ggcttcagtt acccggaatg gaaaggcaag ttctacccgg cagggctgcc ggcttcagag
     4621 atgttgaggt tctactcccg cgcgctcgcc acggtcgaga ttaacaactc gttctaccgg
     4681 tatcccacgg aagaaacgct ccggcagt
//
LOCUS       DBNGSB010000015         3258 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_777425, whole genome shotgun sequence.
ACCESSION   DBNGSB010000015 DBNGSB010000000
VERSION     DBNGSB010000015.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_777425"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="AC159B_00425"
     CDS             complement(<1..823)
                     /locus_tag="AC159B_00425"
                     /inference="COORDINATES: protein motif:HMM:NF024869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIR3376249.1"
                     /translation="MKELLEILDAIQKFAKESQQMALATIIGVRGSTYRREGARLLCT
                     QRQQMIGNISGGCLESDVMVVSEEVMNTRQPRMVTYDLTADDDAVWGLGLGCNGAVDL
                     FVEPVDPASPVLPLLRDAIANEQSLGSITVVEGPAPLGSRLVVYGDGRRVGTLGDGAL
                     DDRADRAAHAALEEGISRVQTLPAVNGEVKVFIEAIRPPIRLVVCGAGHDAIPVVSVA
                     TMLGWRVVVVDQRERYLTHERFPGAREFIKADPAQAGARVPIDNRTYVVVMTHNYL"
     gene            complement(831..1949)
                     /locus_tag="AC159B_00430"
     CDS             complement(831..1949)
                     /locus_tag="AC159B_00430"
                     /inference="COORDINATES: protein motif:HMM:NF017569.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR3376250.1"
                     /translation="MKRSGDLTGHVVGFCRALRARSIPVGPKEAADALTALAAVDLSD
                     RKESYLALRSVLTSRREDLFIFDQVYAEYWNPPQEQQALPHMPEPLPSAGDAQASSPT
                     SLRWLDEALAVDGEQTPAYSPVETLTEKDFSALSTDELDEVARIVIRIARRVATRLSR
                     RMRQSKRGRVDLRRTIRHSLRRGGEIVDLAYRRRKITKTKVVLLADVSGSMDLYSRFL
                     IQFIYALQHTLGHVETLVFSTSLTRVTDALDEADLHRALKEVAKQVPDWSGGTKIGYS
                     LRTFLREHGDQLTSRTVVLIISDGWDTGEMEVLERAMEEIRRRAGRIIWLNPLLGSPG
                     YEPICQGMRVALPYVDVFASAHNLESLRQLEKHLAAKR"
     gene            complement(2100..3050)
                     /locus_tag="AC159B_00435"
     CDS             complement(2100..3050)
                     /locus_tag="AC159B_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3376251.1"
                     /translation="MHPEIVRIQSMLDSQGYVADRDLATSVYLAIQLRKPLLIEGAAG
                     VGKTEVAKVMAKALDTDLIRLQCYEGLDSTTSLYEWNYQRQLLHIRLQENSNLPIEEK
                     EREIFSEPFLLKRPLLAAITHDRAPVLLIDEVDRSDEEWEAFLLEVLSDWQVTIPEIG
                     TIRARHTPYVVLTSNRTRELGDALRRRCLYLWIDYPVFEKELAIVRRRAPGVDGRLAE
                     QIAAFMQFVRRAKLDKVPGIAETLDWSAALMALHRDHLDRDAIEQTLGVLFKHHDDAE
                     LVRTQWLGHLLRGLEEVGREPKPWSQPTIQRIADNVLPRR"
     gene            complement(3061..>3258)
                     /locus_tag="AC159B_00440"
     CDS             complement(3061..>3258)
                     /locus_tag="AC159B_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376252.1"
                     /translation="FELDHTITPSPHNPLGAKGVGEAGTIGATPAVVNAVLDALTPFG
                     VKHLDMPITPRKIWAAMQSSL"
BASE COUNT          601 a         1076 c          954 g          627 t
ORIGIN      
        1 gcaggtagtt gtgcgtcatc accaccacat aggtccggtt gtcgattggc acccgggcac
       61 cggcctgggc cggatcggcc ttgataaatt cgcgcgcgcc tgggaaccgc tcatgcgtca
      121 agtagcgctc gcgctgatcg accacgacaa cccgccatcc cagcatcgtc gcaacgctca
      181 ccaccggaat agcgtcgtgc cccgcgccgc aaaccaccaa acgaataggc gggcggatcg
      241 cttcgatgaa cactttgacc tctccgttca ccgccggcaa tgtttgtacg cgtgagatcc
      301 cctcttctaa tgccgcgtga gcggctcggt cagcgcgatc gtcaagcgca ccgtccccca
      361 gcgtgcccac acgtctgcca tcgccgtaga cgaccagcct cgagcccaat ggcgccgggc
      421 cttcgaccac cgtgatcgac ccgagggatt gctcattggc aatcgcgtcc cgcaagaggg
      481 ggagaacagg agacgctgga tcaacgggct ccacgaagag atccaccgcg ccgttgcacc
      541 caagccccag gccccagacc gcgtcgtcat cagcggtaag atcataggtc accatcctgg
      601 gctgacgtgt attcatgacc tcttccgaca cgaccatgac gtcgctctcc agacatccac
      661 cgctgatgtt gccgatcatc tgctgccgct gtgtgcacaa caggcgtgcg ccctcgcgcc
      721 ggtaggtcga gccgcggacc ccaatgattg tcgcgagggc catttgttgg ctttccttcg
      781 caaatttctg aatggcatcc agaatttcga ggagctcttt catggtctac tcaccgtttg
      841 gccgccagat gtttttcgag ctgccgaaga ctttcgagat tgtgcgccga ggcaaacaca
      901 tcaacgtacg gcagcgctac gcgcatccct tggcagatcg gctcgtagcc gggactgccg
      961 agcaatggat tgagccagat gatacggccc gcgcggcgcc ggatctcctc catggcgcgc
     1021 tcgagcacct ccatttcgcc cgtgtcccag ccgtcgctga tgatcaagac gaccgttctc
     1081 gaggtcagct ggtcgccgtg ctccctgaga aacgtccgca ggctgtatcc gatcttcgtg
     1141 cccccggacc agtcgggcac ctgtttcgcc acttccttca gggcacgatg caggtcggcc
     1201 tcatcaagcg catcggttac ccgcgtcagc gacgtcgaga aaaccagcgt ctcaacgtgc
     1261 ccgagcgtgt gctgcagcgc gtaaatgaac tggatgagga agcggctgta gagatccatc
     1321 gacccgctga catccgccag gaggacgact tttgttttcg tgatcttccg gcggcggtac
     1381 gccagatcga cgatctcgcc gccgcgccgc aggctgtggc gaatcgtccg ccgcaggtcc
     1441 acccggccgc gctttgattg ccgcatgcgc ctgctcaatc gcgtcgccac acgccgtgcg
     1501 atcctgatca caatccgggc gacctcgtcg agctcgtctg ttgaaagcgc gctgaaatcc
     1561 ttctcggtga gtgtttcgac cggactgtag gccggcgtct gctcgccgtc caccgcgagc
     1621 gcctcgtcga gccagcgcaa cgatgtcggc gatgatgcct gcgcatcccc agccgaaggc
     1681 agtggctccg gcatgtgggg cagggcctgc tgttcctgag gcgggttcca atactctgcg
     1741 tagacctgat caaagatgaa caaatcctct cgcctggatg tcagcacgga ccgaagggcg
     1801 aggtatgact cttttcgatc gctgagatcg acggcggcga gcgcggtcag cgcatcggct
     1861 gcctccttcg gacccaccgg aatgctgcgg gcccgcagcg cccggcagaa tccgacgacg
     1921 tgcccggtca gatcgcccga gcgtttcatc ctagtcgcaa cgagcaaaat tcaccggcct
     1981 cgccccaggc tcggccttca ctagtcacaa tacgtacaag ctccaccggt ctcgctgtac
     2041 tcaacctcct caacagccgc cgataacgca aggaaacact agtggtcgcc acctgaaact
     2101 catctgcgag gcagcacgtt gtcggcgatg cgctggatgg tgggctgact ccatggcttt
     2161 ggctcccgac cgacttcttc cagcccccgc aggaggtggc caagccactg cgtccggacc
     2221 agttccgcgt cgtcgtggtg tttgaagagc acgccgagcg tctgctcgat ggcgtcgcgg
     2281 tcgaggtgat cgcggtgcag cgccatcagc gctgcgctcc agtccaacgt ctctgcaatg
     2341 cccgggacct tgtcgagttt ggcccgtcgc acgaactgca tgaaggccgc aatctgctcc
     2401 gccagccggc cgtccacgcc aggcgcgcgg cgccgcacga tggcgagctc tttctcgaac
     2461 actgggtaat caatccacag gtagagacac cgccggcgga gcgcatcgcc cagctcgcgt
     2521 gtgcggttgc tcgtgagcac gacgtatggc gtatgccggg cgcggatcgt accaatctca
     2581 gggatggtga cctgccagtc agacagcacc tcgagcagaa atgcctccca ctcttcgtcc
     2641 gaacgatcga cttcgtcgat caacagcacg ggtgcgcggt cgtgcgtgat cgcggccagc
     2701 aacggtcgct tcaacaggaa cggctcgctg aaaatctcgc gctctttctc ctcgataggt
     2761 agattgctat tctcctgaag ccggatgtgc aggagctggc gctgataatt ccattcatac
     2821 agtgacgtcg tggagtcaag cccttcgtag cactgcagcc ggatgagatc cgtgtccagg
     2881 gccttcgcca tcaccttcgc gacctcggtc ttaccgacgc cggccgcgcc ttcgatcagc
     2941 agcggcttcc gcaactgaat cgcgaggtac accgacgtcg ccaggtcgcg atcggcgaca
     3001 tagccctggc tgtcgagcat cgattggata cggacgattt cgggatgcat gaccttacaa
     3061 ttataaactg ctttgcattg cggcccaaat cttccggggg gtgatcggca tgtccagatg
     3121 cttcacgccg aagggggtga gcgcgtccag gaccgcgttg accacggccg gggtggcccc
     3181 gatcgtcccg gcctccccca cgcctttcgc gcctagggga ttgtgcggcg acggcgtgat
     3241 ggtgtgatcg agctcgaa
//
LOCUS       DBNGSB010000016         3068 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_1852120, whole genome shotgun sequence.
ACCESSION   DBNGSB010000016 DBNGSB010000000
VERSION     DBNGSB010000016.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_1852120"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="AC159B_00445"
     CDS             <1..452
                     /locus_tag="AC159B_00445"
                     /inference="COORDINATES: protein motif:HMM:NF020133.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HIR3376253.1"
                     /translation="RRRSVQSLRDFTMDDLALVEELPDPQRRRVRHVVTENHRVVQAA
                     QALESGQVGPLRDLFLASHQSLRNDYDVSTPELDAMLEAAVASPGCVAARMTGAGFGG
                     SIIALVQQPLQERFLQNVAAEYQHRSQHKGRFFATRAAGGAQILELA"
     gene            complement(449..2023)
                     /locus_tag="AC159B_00450"
     CDS             complement(449..2023)
                     /locus_tag="AC159B_00450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HIR3376254.1"
                     /translation="MYVASAKQIADIDRRTTEEYGIAAGTLMERAGQAVADVAISMLS
                     GNDSPRVVVVCGKGNNGGDGLVAARHLRRANVQVETILLSPESELSGNARRAFAEAQQ
                     DGVVIRAAGASDGGPIFAGADLIIDAIFGTGFKGPARGRAAELISSINHAGKPVLSVD
                     IPSGVSSDTGSADGAAIRASATITMGFLKVGLLVSPGADLAGEILVADIGYPEALSSN
                     SSVSTHFVTSAMVRSCLPVRRPDSHKGDHGHVLIVAGSMGFTGAAVLATRGALRSGAG
                     LVTLAVPKSVYPIIAAQVVEGMPTPLPDSNGALAAGALKQLRVLRGGSDVAAVGPGLS
                     QAAGVRRVVQDLLAADGPVVLDADGLNVLVGKTKILSRASSPLVITPHPGELGRLMNI
                     PAEKVQEDRLSAARTAAKRFKCTVLLKGARTVVATAAGDAYIVPTGNPGMATGGMGDV
                     LTGAIASLIGQGLDPTAAAYCGAYLHGLAADLIASERGMAGILASEVADHLPVAIEHV
                     RLGKVRDPITQLPQNS"
     gene            complement(2339..>3068)
                     /locus_tag="AC159B_00455"
     CDS             complement(2339..>3068)
                     /locus_tag="AC159B_00455"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3376255.1"
                     /translation="ITMLPDSPDVQQVVTGPGGVLEGMRKGAILVDMSTISPMVTAEI
                     AKAVRSKGGEMLDAPVSGGEKGAIEATLSIMVGGPEETFARMKPVFEAMGKNIVHIGA
                     TGAGQVTKACNQIVVALTIQAVGEALALAAKAGVDPGKVRQALLGGFAQSRILDVHGQ
                     RMLERNFKPGFRVRLHQKDLNIGLSTGKSLGVPLPATAVVQEAFTALLGLGRGDQDHS
                     SLVTVIEDLAKTQVKAHERIEGRS"
BASE COUNT          584 a          972 c          915 g          597 t
ORIGIN      
        1 agagacggcg ttccgtacag tcgttgcgcg attttacgat ggacgacctc gcgttagtcg
       61 aggaacttcc cgatccccaa cgccggcgtg tacgacacgt ggtcaccgag aatcaccgcg
      121 tcgtccaggc cgctcaggcg ctcgaatccg gacaggttgg cccgctgcgc gatctcttcc
      181 tcgcgtcaca tcagagttta cgcaacgatt acgacgtcag tacgccggag ctcgacgcaa
      241 tgctggaggc tgctgtcgcg tcccccggct gcgttgcggc gagaatgacg ggcgcgggtt
      301 tcggcggatc gatcatcgca ctcgtacagc agccgttgca ggagcggttc ctccagaacg
      361 tcgcggcgga atatcaacac cgcagccagc ataagggcag gttctttgcg actcgggccg
      421 ccggtggagc gcaaatactg gagttggcct aggagttctg ggggagctgg gtgatcggat
      481 ctcttacctt gccgagcctg acgtgctcga tcgcaaccgg caggtgatcg gcgacctctg
      541 aggccaggat gccggccatc ccacgctctg aagcgatcag atcggcggca aggccgtgga
      601 ggtatgcgcc gcaatacgcc gcggccgtcg ggtcaaggcc ctggccgatc agcgacgcga
      661 ttgctccggt caggacatcg cccattcctc ccgtcgccat tccgggattt cccgtcggca
      721 caatgtatgc atcgccggca gctgtagcta caacggtcct cgctcctttc agcagcaccg
      781 tacacttgaa ccgttttgcc gccgtgcgag ccgcgcttag acgatcttct tgaacttttt
      841 ccgctgggat attcatcagg cgtccaagtt ctcccggatg aggtgtaatg acgagtggcg
      901 aggaagctct ggataagatc ttggtttttc caacgagcac gttgagccca tcggcgtcaa
      961 gaacgacggg gccgtcggcc gcgagcaggt cttgcaccac gcggcggacg cccgcagcct
     1021 gagaaagtcc cggaccgaca gccgcaacat cgcttccccc tcgcagaacc ctgagttgtt
     1081 tcagagctcc cgcagccagc gcgccgttgc tgtcgggcaa cggtgttggc atcccttcaa
     1141 ccacctgcgc ggcaatgatt gggtagacgc ttttggggac ggcgagcgtc accagccccg
     1201 ccccggatcg cagcgcaccc ctcgtcgcca gcacggcggc gcccgtgaaa cccatggagc
     1261 ctgccacgat caggacgtga ccatgatccc ccttgtgcga atccggtctg cgcacgggca
     1321 gacaggaacg caccattgcg gaggtgacaa aatgagttga aacggatgag ttactcgaca
     1381 gtgcttctgg atatccgatg tccgcaacaa gaatttcgcc ggcaagatcg gctcccggag
     1441 aaaccagcaa accaaccttg agaaatccca tggtgattgt cgcggaggcg cgaattgctg
     1501 ctccgtctgc gctgcccgta tcggaggaca cgcccgacgg aatatcaacg gagaggaccg
     1561 gttttccggc gtgattgatg gagctgatga gttctgcggc gcgccccctg gcaggaccct
     1621 tgaatccggt tccaaagatg gcgtcgatga tgaggtcggc gccggcgaaa atgggtccac
     1681 catcggaagc acctgcggct cggatcacga caccatcctg ctgtgcttct gcaaaggccc
     1741 tgcgggcgtt gcccgagagc tcggattcag gtgacaacag aatggtctcc acttggacgt
     1801 tcgccctgcg caggtgccgc gccgccacaa gtccgtcgcc gccgttgttt cccttgccgc
     1861 atacgacgac gacgcgggga ctgtcgtttc cggacagcat cgagatcgcg acgtcagcga
     1921 ctgcctgtcc ggcgcgttcc atgagcgtcc ccgcggcaat gccgtattcc tcggtcgttc
     1981 ggcggtcgat gtcggcaatt tgcttcgcac tcgcaacata catcgttctc aagtctactt
     2041 caacgttatc ccccgaggac acactatcca cagccggtga acgaaggttc tcgattgacc
     2101 gactatacag gccatggtag cctgcaatac gagaggcgcc agcctaacgg cgaagaagcc
     2161 gagtcaaacg ctgtggcgcg tggactctga gagccagctg tccttcactt cgggagtaat
     2221 cccggagagg agccagcagg cagtaggaac cggcggctgc agctgaagac ggtgaagaag
     2281 ccggataaag ccagcgcctc tcactatttg tctgcacggt ccacacagtc tgcacggcct
     2341 acgatctacc ttcgattcgc tcatgggcct tgacctgggt tttggcgagg tcttcgatga
     2401 cggtcacaag ggacgagtga tcttggtccc cccggcctaa tcccaggagt gccgtaaacg
     2461 cttcttgcac gacggcggtc gccgggagcg gcacgcccag actcttccca gtcgacaatc
     2521 cgatgttgag gtctttctgg tggagccgga cacgaaatcc cggcttgaaa tttcgttcga
     2581 gcatccgctg gccatgcacg tccaggatgc ggctctgggc gaatcccccg agcaatgcct
     2641 ggcgcacctt gcccggatcg acgcccgctt tcgccgcgag cgcgagcgct tcgccaaccg
     2701 cctgaatcgt caaggcgacc acgatctgat tgcaggcttt cgtgacctga ccggcgccgg
     2761 tcgcgccgat gtgcacgatg ttcttcccca tcgcctcgaa gaccggcttc atccgcgcga
     2821 atgtctcctc gggaccgccc accatgatgg acagagttgc ttctatcgcg cctttctcgc
     2881 cgccgctcac gggcgcatcc aacatctccc cacctttgga ccgcacggcc ttggcgattt
     2941 ccgcagtgac catgggggag atcgtgctca tatcgacaag gatggcacct ttgcgcattc
     3001 cttccaggac gccgcctggg ccggtgacga cctgctgcac gtctggtgaa tcaggaagca
     3061 tcgtgatc
//
LOCUS       DBNGSB010000017         3028 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3078891, whole genome shotgun sequence.
ACCESSION   DBNGSB010000017 DBNGSB010000000
VERSION     DBNGSB010000017.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3078891"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="AC159B_00460"
     CDS             <1..469
                     /locus_tag="AC159B_00460"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR3376256.1"
                     /translation="ALNPGNSGGPLVDFQGRVVGINTAVIMGSQGICFAIPVNTAKWV
                     TSLLIREGKVRRSYLGVSGQPVQIDRRLALEFTLKDTGVRVIEVQPNTAAESAGITTG
                     DIIIKAGDATVATPDDLQRILGKHPIGDVLAVEVLRGSERRLLTTRPSELPDN"
     gene            complement(487..1560)
                     /locus_tag="AC159B_00465"
     CDS             complement(487..1560)
                     /locus_tag="AC159B_00465"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3376257.1"
                     /translation="MITVGEVMTRNPVMLGAGATLREALWTMRDRGISSVLVVPQPGS
                     AEYGILTMRDVIAKVVGPGLDPVRVRVGDVVSWRLTTAEPDWPLQQAAATMARAKVRR
                     LPVASGDELIGIVSDTDLFAAIVPGDGWEHARAVRKERALRRVHQNGAVRTVADLMSS
                     PVLTIEPDAKVVDAVRKMASSGVSSLIVQRGDDAEGIITKRGVITKLMAEGKALEDTR
                     VADVMSAPVRMIDASATIEACSARMVEENVRRFPVTREGRIAGIISDKDILSAVLAER
                     WRANRRVPASFIVADVMRPAVGVTETAGTEGLVPEMNLWDAADRIAAAATRRLPVVQA
                     GKIIGTVGEAEILLALEDRGAGD"
     gene            1739..2452
                     /locus_tag="AC159B_00470"
     CDS             1739..2452
                     /locus_tag="AC159B_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376258.1"
                     /translation="MDNLRRIGGEAGVLAGIAMAWLFLGLVLVFPSTGLSLADQANPH
                     RYISFIAKHEAAFWAVNVLGGFVAGVFSAVLILALGDRFREASPGSTRIATEFGVFGA
                     AAFACAAIVRQVGFASLTSIYKATTTGGAAAFYAMNGVVDALIALSGIAMGFGILLLG
                     SVMAKSERYAAVGTLSLVAGAAMVLGGFINNIYLYMASSVAAIVWLTWTALSLRAEVG
                     PALFNFAVRRNGRSRAKAA"
     gene            2641..>3028
                     /locus_tag="AC159B_00475"
     CDS             2641..>3028
                     /locus_tag="AC159B_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376259.1"
                     /translation="MRKANALLGTLIALALIVAWLPGPAYAQVVTTETSTFVNRFGNT
                     VTTINTFHDGILVFRDIQEVRPSGVVTLEKHWDFYSDGTVKSYTEERLDGFAPYLIER
                     QYDQNGVLRSYHADLYMNGELYLQTFQ"
BASE COUNT          556 a          943 c          914 g          615 t
ORIGIN      
        1 ggcgctcaac ccggggaact cgggcggacc gctcgtcgac tttcaaggac gtgtcgtcgg
       61 catcaacacg gccgtcatca tgggatctca gggcatctgc tttgcgatcc ccgtcaacac
      121 cgcgaagtgg gtcacgtcac tcctgatccg cgaagggaag gtccgccgct cgtatctggg
      181 tgtttcaggt cagccggtgc agatcgatcg ccgtctggcg ttggagttca ctttgaaaga
      241 caccggcgtc cgcgtgattg aggtgcagcc gaatacggcg gccgaatccg cggggatcac
      301 gaccggcgat atcatcatca aagcaggcga cgccaccgtc gccacaccgg acgacctgca
      361 gcgaatcctt ggaaaacatc ccatcggaga tgtgctggcg gtcgaggtcc tgcggggaag
      421 tgaacggcgc ttgctaacaa cgagacccag cgagttacca gataactgac cgactaattt
      481 gaggggttag tcacctgcgc cgcggtcttc gagggcgagc aggatctcgg cctcgcccac
      541 tgtgccgatg attttgcccg cctgaaccac ggggagccgt cgcgttgctg cggcggcaat
      601 ccgatcggcc gcgtcccaga ggttcatctc cggcaccagg ccctcggtgc ctgccgtctc
      661 cgtgactccc accgccggcc gcatcacgtc ggcgacgatg aaggatgccg gcacgcgccg
      721 gttcgcgcgc cagcgctcgg ccagcaccgc cgagagaatg tccttgtcgc tgataatgcc
      781 tgcgattctg ccctcgcgtg tgactggaaa acgccgcacg ttttcttcga ccattcgcgc
      841 cgagcatgcc tcgatcgtgg cactcgcgtc gatcatccgt accggcgccg acatgacatc
      901 tgccacgcga gtgtcttcaa gcgctttgcc ttctgccatt aattttgtga tgacgccgcg
      961 tttggtgatg atcccttccg catcgtcacc acgctgaacg atgagactgc tcaccccgct
     1021 cgacgccatc tttcggaccg cgtcgacgac tttggcatcg ggctcgatcg tcagcacggg
     1081 tgaggacatc agatcggcaa cagtccgcac cgctccattc tgatgaacac ggcgcagggc
     1141 gcgttccttg cgcacggcgc gggcatgctc ccagccatcg cccggaacga tggcggcgaa
     1201 caggtcggtg tcgctgacga taccgatgag ctcatcgcct gacgcgactg gcagtctgcg
     1261 gacctttgcc ctggccatcg tggccgcggc ctgttgaagg ggccagtcgg gctcagcggt
     1321 ggtcagccgc caggagacca cgtcgcccac ccggacccgc accggatcaa ggccgggacc
     1381 cacgaccttg gcgatgacgt cgcgcatggt gaggatgccg tattcggcgg aaccgggttg
     1441 aggaaccacc aggacgctgg aaataccacg gtcccgcatc gtccagaggg cctcacggag
     1501 ggtagcgccg gcgcccagca tgacggggtt ccgggtcatc acctcgccaa ccgttatcat
     1561 gtgtcccccc ttgagtaccc atatctttac ccaggcccca agcgcctgtc tatccctcgt
     1621 ctgacgggtt ccctttcctc taaacggaga ctaactgcgg acttcggggg gcatgagata
     1681 cctgccacgc agactggttc gtagtacgtt tctgcgccat caaaggaggg gtatccgcat
     1741 ggataacctg cgacgtatag gtggcgaagc tggagtgctg gccgggatcg cgatggcctg
     1801 gctctttctg ggacttgtcc tcgtctttcc ctcgacaggc ctcagcctag ccgaccaggc
     1861 gaacccgcac cggtacatct cgttcatcgc caagcatgaa gccgccttct gggcggtcaa
     1921 cgtcctgggt gggttcgtcg ccggcgtgtt cagcgccgtg ctgatccttg ccctgggcga
     1981 tcggttccgt gaggcctcac cgggcagcac gcgaatcgca acggaattcg gggtgttcgg
     2041 agcagcggcg ttcgcctgcg ctgcgattgt aaggcaggtc ggatttgctt cgttgaccag
     2101 tatttacaag gctacaacca ctgggggagc cgctgccttc tacgcgatga acggcgtcgt
     2161 cgacgccctg atcgcgctga gcgggattgc aatgggattt ggcattctcc tcttgggaag
     2221 tgtcatggcc aagtcggagc gctatgcagc cgtcggtaca ctgagtcttg tggccggcgc
     2281 tgcgatggtt ctgggtggtt tcattaacaa catctacctc tacatggcaa gctctgtcgc
     2341 tgcaatcgtc tggctcacct ggaccgcgct ctcgcttcgg gcggaagtcg gcccggcact
     2401 gttcaatttc gccgttcgac ggaatggacg gtcaagggcc aaggccgctt gatttgcaag
     2461 tctgacgtgg cgcacggcaa tgccgtgcga ggcaacggaa gcccgaggga agcctcgggc
     2521 ttcctacttc tgcggctatg ttccctgtaa cttcggtgga agtctcatag gattactctg
     2581 gcacgctcct tgcttcctta gtcattaagt agaaagggta cgcacaagga gcgaggacac
     2641 atgaggaagg caaacgcact tctcggtacg ctcatcgcgc tcgcgttgat tgttgcctgg
     2701 ttgccgggtc cggcctacgc gcaggtcgtc accacagaga cctccacgtt cgtcaaccgg
     2761 ttcgggaaca cggtcaccac aatcaacacc ttccacgacg gtattctggt cttccgcgac
     2821 atacaggagg ttcgccctag cggcgtcgtg acgctggaaa agcattggga cttctactct
     2881 gatggtaccg tcaagagcta cacggaagag cgactggacg gtttcgctcc ctacctgatc
     2941 gagcggcagt acgaccagaa tggcgtcctg cgcagctatc acgctgacct ctatatgaat
     3001 ggcgagctct acctacagac attccaga
//
LOCUS       DBNGSB010000018         5430 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2006549, whole genome shotgun sequence.
ACCESSION   DBNGSB010000018 DBNGSB010000000
VERSION     DBNGSB010000018.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5430)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2006549"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            358..1983
                     /locus_tag="AC159B_00480"
     CDS             358..1983
                     /locus_tag="AC159B_00480"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3376260.1"
                     /translation="MDRMKRREFLKRVGIGVGALATSQALPRLFVPGAYAQSTGTLTY
                     GMAGGFDTLDVTATTFTRVGRIALHIIDPLVWSTGSGQYAPGLATSWTASPDATVYTF
                     KLREDVRFHDGTPLTAEAVKVTFDRIVDPATRAQTAFSFIGPYDRSDVLDRYTVRVRF
                     KSPHASFLNGASSPYLGIISPTAVQRFGQDFGRLVFIGTGPFMLQSYRTDAEVRLIRN
                     PNYTWGAPSFNHQGRAYLESIVYRIVPEEATRLATLETGETLFIEDVPVTDYQRLKSS
                     QNKVLLEIRQAGSGHSLMMNQMRPPTDELAVRRAVMHAIDKEGLVRTVWNNVYKAGCS
                     PLTPNMLGFDAETCRKVPYNQDQARRILDEAGWRVGPDGIRVKGSERLRLEFYLQTTP
                     QKNIEMAQFIQANAKAVGIDVNLNVIARAGYLDAVRQGRHNFQFWWDTGTDPGQMLRI
                     LFHSRNAGGGTNRNNYRNQDMDRLIEQIDGISDPTRRKEIIVRAQQKAIDDAVIVYLA
                     DPPSLYAHDRSVSGVWVDWGGNYPYFFNTRVQR"
     gene            2047..2973
                     /locus_tag="AC159B_00485"
     CDS             2047..2973
                     /locus_tag="AC159B_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011228608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3376261.1"
                     /translation="MLQFVLRRLLLAIPTMVAASILVFLMLHLVPGDPAIVFFGDQPV
                     TPERLEEVRRQMGLHRPLVVQYLDFVRGIPRGDLGRSIHNRTPVLEELRTRVGSSAEL
                     ALAAFVLAAVLGLTLGLLSALWRGKWIDAGAMLVALGGVSMPIFWLASMLIFVFSLKL
                     GWFPAIGFGGGWNRLILPATALGFISSATLARLVRSSVLEVLLQPFVTTARSKGLREA
                     VVIQRHVLKNAFIAVITVMGLQFGTLLSGAVITETVFARPGVGKLLVDAISNKDFPIV
                     QGAVLFIALVYIMINLLVDLSYAYLDPRIRFE"
     gene            2939..3841
                     /locus_tag="AC159B_00490"
     CDS             2939..3841
                     /locus_tag="AC159B_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009225140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3376262.1"
                     /translation="MRTSIPASVSSKAPVVILAASRSPSREVLRRLRRSKSAALGGFI
                     LAALVVAAFAAPLLTPYEPLVMNPVDRLQPPNARHWLGTDVFGRDLLTRILFGSRISL
                     QTGLISVTIAILIGVPLGLVSGFYGGLIDRSAMRVVDLMLTFPGILLALVLIAILGPD
                     LFNAMLAVGISASPTFARVVRAAVLSSKEQTYVEAARAVGCTNARIMLRHILPNTVAP
                     IIVLGTLGVAGAIIAAAALSYLGLGARPPQPEWGALLSEGRNYLRVAWWMTTFPGLAI
                     MTAVLSINLLGDGLRDALDPRLQS"
     gene            3875..4996
                     /locus_tag="AC159B_00495"
     CDS             3875..4996
                     /locus_tag="AC159B_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019587662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3376263.1"
                     /translation="MKNRKLRVGVLGAGAWATFAHIPGWMRDPRCEVVTVCDVIRDRA
                     EEVAARFGIGEAAPDWQTVVSRTDIDVIDVVTPSSTHQELALAALGAGKHVLCEKPVA
                     YDFRDTWGAAELAKRKGLLTKLGFTFRYTPGVQYAKSLIADGFVGEPFFFNGYEQNSQ
                     WLDPKTPLRQVDHTADQSVLQTSSLEGYGAPIIDIGMWWIGAEYARCVGTMRNFIPER
                     EVRATGQMMRMNIDDGDIHLVEYTNGALGSIQTSFVTVGNYPGVEARIYGSKGAIICR
                     AVEEFGVAETIKVATPDAVEFKQVEIPERFYPAGGTPHESWRSLFYANLIKDFTDEIA
                     DGHGRNQGNFEDGARVQEVINAVERSYHERRWIELPLPR"
     gene            4999..>5430
                     /locus_tag="AC159B_00500"
     CDS             4999..>5430
                     /locus_tag="AC159B_00500"
                     /inference="COORDINATES: protein motif:HMM:NF017745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HIR3376264.1"
                     /translation="MDAPAFASALDAFFDWYYRTYPVNATFIGNHNHDHRLPDFSENG
                     AGDAQAALDALRARLRRLPPEPLTNAERMDRILVEGALDIQGWESGSRHFQRGNPCVY
                     TGEAVFGVMALLLRPFAPLEARVEAAVERMMAIPTFLEQGRA"
BASE COUNT          971 a         1663 c         1717 g         1079 t
ORIGIN      
        1 gccattgccc gcatccctcc ttagacctgc cgctgcagtg tgtccctcgc cattacctga
       61 tgattctcga atcctgtgcg atcatgaaag acaatatgac ccctgaaacc ccgattgagg
      121 ccaggaaggg tcgatttact ggtccttatt tgtcccgcaa agtgcgtagg ctgacgctac
      181 catagacttt ctctaactgt ctacctgtca ttggagggat tttcgaaaca cattcggacc
      241 ataccaggag gtgagggact cgaccttatc gaggtccttt cctcctgtat ttgcaaggtt
      301 aagggccgac ggggcgagaa ttacacagcc cacattggtc aaagggggat tttcataatg
      361 gatcgcatga agcgacggga gttcctaaag cgggtcggca tcggcgtcgg cgcgctggcc
      421 acgtctcagg cgctgccgcg cttgtttgtg ccgggcgcgt acgcgcagag caccggcact
      481 cttacctacg gcatggcagg cggattcgac acactggacg tgaccgcaac gaccttcacg
      541 cgcgtgggtc gcatcgccct gcacatcatt gacccgctcg tctggtcgac cggctcgggg
      601 cagtatgctc cgggtcttgc cacctcgtgg actgcctcgc cggacgcgac cgtttacacg
      661 ttcaagctgc gcgaggacgt gcgtttccat gacggcacgc cgctcaccgc agaagccgtg
      721 aaggtcactt tcgaccgaat cgtggatccc gccacgcgcg cccagaccgc gttctccttt
      781 atcggccctt acgatcgatc cgacgtcctg gacaggtaca cggtccgcgt gaggttcaag
      841 agcccccacg cctcgttcct caacggcgcg agcagcccgt acctcggcat catctcgccg
      901 acagcggtgc agaggtttgg gcaggacttc ggccgtctcg tgttcatcgg caccggtccc
      961 ttcatgttgc agtcataccg tacggatgcc gaagtgcggc tgatccgcaa ccccaactac
     1021 acctggggag ccccgagctt caatcaccag ggacgcgcgt atctcgaaag catcgtctac
     1081 cgtattgttc ctgaggaagc gacgcggctt gcgaccctgg agaccggcga gaccctcttc
     1141 attgaggacg tgccggtaac cgactaccag cgattgaaaa gctcacagaa caaggtgctg
     1201 ctggagatca ggcaggcggg ctcaggccat tccctgatga tgaatcagat gcggccaccg
     1261 acggacgagc ttgccgtgcg gcgcgccgtg atgcacgcca tagacaagga aggcctggtc
     1321 agaacagttt ggaacaacgt gtacaaagcc gggtgcagcc ctctcacgcc caatatgttg
     1381 ggcttcgacg ccgagacgtg cagaaaagtc ccgtacaacc aggaccaagc aaggcgtatc
     1441 ctggacgaag ccgggtggcg ggtgggaccc gatggcatca gggtgaaggg cagcgaacgc
     1501 ttgcggctcg agttctatct ccagacgacc ccgcagaaga acatcgaaat ggcccagttc
     1561 atccaggcca acgccaaggc ggtcgggatc gacgtcaact tgaacgtgat cgcgcgggcg
     1621 ggctatttgg acgcggtgcg tcagggccgc cacaacttcc agttctggtg ggacaccggg
     1681 accgacccag gccagatgct gcgcatcctc ttccattcac gcaacgccgg cggcgggacg
     1741 aaccggaaca actatcgcaa ccaggacatg gaccggctca ttgagcagat tgacggcatt
     1801 tcggatccaa cgaggcgcaa agagatcatc gtcagggcgc agcagaaggc cattgatgat
     1861 gcggtgattg tgtatctggc cgatccaccg tcgctctatg cgcatgaccg gtcggtgtct
     1921 ggcgtgtggg tcgattgggg cggcaactac ccgtacttct tcaatacccg cgtccagaga
     1981 tagtccctac agtcgcaggt ctgggagccg gcagcctctc gccgccggct cccagctaac
     2041 gcgctgatgc tccagtttgt gctcaggcgt ctgctgctcg ccattcccac gatggtcgcg
     2101 gcatccattt tggtgtttct catgctgcat ctggtgcccg gtgacccggc catcgtcttc
     2161 ttcggcgacc aaccggttac accagagcgg ctcgaggaag tccggcgcca gatgggactg
     2221 caccgcccgc tggttgtgca atatctggat ttcgtgcgtg ggattcctcg aggggatctc
     2281 ggccgctcca tccacaaccg cacccctgtg ctcgaggaac ttcgcacccg cgttgggtca
     2341 tcggccgaac tggcgctggc cgcattcgtc ctggctgccg tgctgggcct caccctcgga
     2401 ctcctgtcgg ccctctggcg cggaaagtgg attgacgcgg gggcgatgct cgtagcattg
     2461 ggcggtgtgt cgatgccgat cttctggctc gcgtcgatgc tcattttcgt cttctcactg
     2521 aagctgggat ggttccccgc cattggcttc ggcggcggat ggaaccggct gatcctcccc
     2581 gcgaccgcgc tggggttcat ttcctcggca acgctggcgc ggctggtgcg atccagcgtg
     2641 ctcgaggtgc tcttgcagcc gtttgtcacc acggcgcgct ccaaaggtct gcgggaagcc
     2701 gtcgtgattc agcggcatgt gctcaagaac gcgttcatcg ccgtcatcac ggttatgggc
     2761 ctgcagtttg gcacgctgct ctctggagca gtgatcaccg agacggtatt cgcgcggccc
     2821 ggcgtcggca agttgctcgt ggacgcgatt tcaaacaagg attttccaat cgtccagggc
     2881 gcggtgctgt tcatcgcctt ggtgtacatc atgatcaacc tgcttgtcga cctctcgtat
     2941 gcgtacctcg atccccgcat ccgtttcgag taaggcgccg gtcgttatcc tggccgcgtc
     3001 acggtcgccg tcgcgcgagg tgctccggcg gctgcggcgc tcgaagagcg cggcgctggg
     3061 aggcttcatt ctggcggcgc tcgtggtggc cgctttcgcg gcgccgctgc tgacgcccta
     3121 tgagccgctg gtgatgaatc ccgtggaccg cctgcagccg cccaatgcgc ggcactggct
     3181 gggtaccgat gtcttcggcc gtgacctgct cacccggatc ctgttcggtt cgcgcatctc
     3241 gttgcaaacc gggctgatct cggtgaccat cgcgatcctg atcggcgtgc cgctggggct
     3301 ggtctcggga ttttacggcg gcctcatcga tcggtcagca atgcgtgtcg tcgacctcat
     3361 gctgacattt ccggggatct tgctggcgct ggtgctcatc gcgatcctgg gacccgacct
     3421 gttcaatgcg atgcttgccg tcggcatttc ggcgtcgccg acattcgccc gagtcgtgcg
     3481 agccgcagtg ttgtcgtcga aagagcagac gtatgtggaa gcagcgcggg cagtcgggtg
     3541 cacgaacgcg cgcatcatgc tgcgccacat cctccccaac acggtggcgc cgatcatcgt
     3601 gctggggacg cttggcgtgg ctggcgcgat catcgcggca gcggcgctga gctatctggg
     3661 actgggtgct cggccgcccc agccagagtg gggagcgctg ctatccgaag gacgcaacta
     3721 cttgcgtgtg gcgtggtgga tgacgacgtt tcctggtctg gcgatcatga ccgcagtgct
     3781 ctcgatcaac ctgctgggcg acggcctgcg ggacgcgctg gatccgcggc tgcagtcctg
     3841 atgaggtgat ggggaggccg aggaggtcgg ggtgatgaaa aaccggaagc tgcgggttgg
     3901 cgtattgggc gccggagcgt gggcgacgtt cgcgcatatt cctggatgga tgcgcgatcc
     3961 gcggtgcgag gtcgtgaccg tgtgcgatgt catccgggac agggccgaag aggtcgccgc
     4021 gcggttcgga atcggcgagg cggcgcccga ctggcagacc gtggtctcgc ggacggacat
     4081 cgacgtcatc gacgtggtca cgccgtcgtc cacccaccag gaactggcat tagctgcgct
     4141 gggggccggc aaacacgtgc tgtgcgagaa accggtcgcc tacgacttcc gcgatacctg
     4201 gggcgcggca gaactggcga agcgcaaggg cctcttgacc aaactcggct tcacgtttcg
     4261 ctatacccca ggggtgcagt acgcgaaatc gctcattgcg gacgggttcg tcggcgagcc
     4321 atttttcttc aacggctacg agcaaaactc gcagtggctc gaccccaaaa cgccgctgcg
     4381 ccaggtggat cataccgccg atcaatctgt gctgcagacc tcgtcgcttg aagggtacgg
     4441 cgctccgatc atcgacatcg gcatgtggtg gatcggggcc gagtatgcac gatgtgtcgg
     4501 cacaatgcga aatttcatcc ccgagcggga ggtgcgggcg accggccaga tgatgcgcat
     4561 gaacatcgac gatggggaca tccacctggt ggagtacact aacggcgccc tcggctcgat
     4621 ccagacgtca ttcgtcaccg taggaaacta cccgggggtc gaggcgcgca tctatggcag
     4681 taagggagcg atcatttgcc gcgcggttga ggagttcggc gtcgccgaaa ccatcaaggt
     4741 ggcaacgccg gacgctgtgg agttcaagca ggtcgagatc ccggagcgat tctaccccgc
     4801 gggcggaacc cctcacgaat cgtggcggtc gctcttttac gccaatctca tcaaggactt
     4861 caccgacgaa atcgccgatg gtcatggccg caaccagggc aacttcgagg atggcgcccg
     4921 cgtgcaggaa gtgatcaacg cggtcgagcg ctcgtaccac gaacgacgat ggattgagtt
     4981 gcccttgcct cgataggtat ggacgcccca gccttcgcgt ctgcgctgga cgcgttcttt
     5041 gactggtact accggacgta ccctgtcaac gccaccttca tcggcaacca caaccatgac
     5101 caccgtttgc ctgacttctc ggaaaacggc gccggtgatg ctcaggcagc ccttgacgct
     5161 ctacgagccc gacttcgccg attgccaccc gagccattga ccaacgccga gcggatggat
     5221 cgcatcctgg tggagggagc tctcgacatc cagggttggg agtccggctc gcgccacttc
     5281 caacgtggca atccgtgcgt gtacacaggc gaggcggtct tcggggtgat ggcactcctg
     5341 ctgaggccat tcgcgccgct ggaggcgcgg gttgaggccg cggtcgagcg aatgatggcg
     5401 atccccacgt tcctggagca aggtcgtgca
//
LOCUS       DBNGSB010000019         6427 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_166664, whole genome shotgun sequence.
ACCESSION   DBNGSB010000019 DBNGSB010000000
VERSION     DBNGSB010000019.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 6427)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6427)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6427
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_166664"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="AC159B_00505"
     CDS             <1..414
                     /locus_tag="AC159B_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3376265.1"
                     /translation="VSCHSFIRMARLAAPLMADGGTMFAMSYHGAQKVVASYNVMGPI
                     KAALEAACRYLAYELGPRKIRVHPISPGPLKTRAASGLKDFDLLLNEAVQRAPVGELV
                     DIMDVGFACAYLATPYARRMTGQTLYVDGGVSIMA"
     gene            417..986
                     /locus_tag="AC159B_00510"
     CDS             417..986
                     /locus_tag="AC159B_00510"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005666701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3376266.1"
                     /translation="MERANPPVPGNPESAASEFPPVRLTLRDGRSVTLRAVRPDDADA
                     MRAALDGLSAEARYSRFMGAIKVTPALVERAVRPPADRDRALVAVAGEGTDATIVGGA
                     RYIGGVDGATCEFAVTITDGWRGSGLASSMMRALIRDAAAHGLKRIEGFVLARNTPML
                     NLARRLGFQVASSEEGPTVKLVRLDLESD"
     gene            1103..1381
                     /locus_tag="AC159B_00515"
     CDS             1103..1381
                     /locus_tag="AC159B_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376267.1"
                     /translation="MSDTSEDLGLVTVLLQRLQQQRLPRALALKEKVDRGERLEDFDI
                     AFLEGVFADANEIKPLVERHPEHQALVSSVVNLYKEITNRALENEKGS"
     gene            1614..2633
                     /locus_tag="AC159B_00520"
     CDS             1614..2633
                     /locus_tag="AC159B_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376268.1"
                     /translation="MFQLFQSIFGPSPPEQGRYPRQLVEAAIERALDATDPRLRFLSG
                     YQKKLRPAVIHAIDQVMSLVDGLPPPVEVSRAVFRADSRLPAIFVSADHLQQVVSADR
                     ALADFLARPGGLATERVVAVLLMQKEERNVLGMELQGEMVMREVPQVTVSFTGHRLLD
                     PTAAEEETRRLLKRRAFDHLLALALGRIASAHAERVELEGQRTLLRHKLATLESGRWS
                     FEPAGAGDTTKVAAIEAQLGEIEGQLRELGADAGTLRLHLDILIEVLGRAQEQLWSKR
                     ISLIVDRMGIKRKRATDTAPEIVLDELHSASGRTAIMLPVSIPPGELRPRSDLLAEAQ
                     RYLGL"
     gene            complement(2636..2836)
                     /locus_tag="AC159B_00525"
     CDS             complement(2636..2836)
                     /locus_tag="AC159B_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376269.1"
                     /translation="MLAASSINTLSYLQMSRVLTANMTEHTVLFGIALVEGKAEVTAL
                     ACPPIRSRPCFALTFLVPAWPG"
     gene            complement(2882..3880)
                     /locus_tag="AC159B_00530"
     CDS             complement(2882..3880)
                     /locus_tag="AC159B_00530"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HIR3376270.1"
                     /translation="MKTAVFSAKRYEREFLDAANASHGHELVYLDRLLEPETAPLAAG
                     CQAVCIFVNDKADAAALRTLAGGGTRFVTTRSTGFNHIDLKAAAALGIKAARVTDYSP
                     YSVAEHAVALLLAVNRKVHRAYNRTRDGNFALDGLIGFDLNGKVVAVVGTGKIGRVFA
                     RIMRGFGCEVLGYDKYPSTEFEALGGRYRDRKEIGERADVISLHCPLTPETHHIINAQ
                     TLARLKRGAILINTSRGALVDAEAAIEAIKSGRLGGLGLDVYEQEAHIFFQDLSSEVI
                     TDDVIQRLMAFPNVIVTGHQAFFTREAVTTICETTIANLSEFAAGKPLTSEIKAGR"
     gene            complement(3965..4153)
                     /locus_tag="AC159B_00535"
     CDS             complement(3965..4153)
                     /locus_tag="AC159B_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376271.1"
                     /translation="MRAHEQIVKYLLAGADVVMTAPALPTAVKAGASFTATGIRGGAP
                     SRHSKLLYTPDNAVSIGL"
     gene            4317..5912
                     /locus_tag="AC159B_00540"
     CDS             4317..5912
                     /locus_tag="AC159B_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-alanine antiporter-like transporter"
                     /protein_id="HIR3376272.1"
                     /translation="MEWLKALLESQPLFALFLTIALGYLLGEVNIKGLSLGSGAVLFV
                     ALAIGAIAPKSAPPALLGTLGLLLFLYGVGLGYGKEFFRGLSSSQGLKANFAALCGLA
                     GALAVTMIAVELAGVKLGYALGTFAGAGTSTATLQAVITALKSDDPAVGYSVAYPIGV
                     AVPILILYLGNAILKPKIPPPAGSRLEFVEVAVRSDKVIGKPFASVKAKFPVNMDVGL
                     IRREHRNIVPTETMSLQADDVLLLISTDKAALARVAQEIGEVQAGRVTNDRTDLDYIR
                     VFASKRTVVGVPLSTLKLPMESECRLLHVRRGDADLLPHDDLILEFGDRVGLLTPREH
                     IPELRRYFGDSIKGTAEFSYISIGIGAAIGLAIGMIPIPIPGVGKLSLGLAGLLLFAL
                     WLGKVRRTAGVTWTMPLSANLVLRNFGLTVFLAQVGMSSGQKFVNTVAATGWTFLVLS
                     VLIVTVLVLLTMIVCMVFFKMPYDEVGGIVSGVTGNPAILAFAARITPTDKPDIGYAM
                     IFPSMTVVKIIIVQVAAVLVGAG"
     gene            6165..>6427
                     /locus_tag="AC159B_00545"
     CDS             6165..>6427
                     /locus_tag="AC159B_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376273.1"
                     /translation="MAIHYCVLLGVVAIIVTACASQTASKTPPQHQLVVESRDKLPAD
                     MRQCTQQHGYDPAKVAGVAESALAPQELPWRQCVYDALRVYARA"
BASE COUNT         1148 a         1990 c         2094 g         1195 t
ORIGIN      
        1 gtctcgtgcc actcgttcat ccgcatggcg cggctcgcgg cgccgctcat ggccgacggc
       61 ggcacgatgt tcgcgatgag ctaccacggg gcgcagaagg tcgtggccag ctacaacgtg
      121 atggggccca tcaaggcggc gctggaagcc gcgtgccgct atcttgccta cgagctgggg
      181 ccgcggaaga tccgcgtgca cccgatctcg ccggggccgc tcaagacgcg cgcggcgtcg
      241 ggactgaagg atttcgacct gctgctgaac gaggccgtcc agcgtgcgcc ggtcggcgag
      301 ctggtggaca tcatggacgt cgggttcgcc tgcgcgtatc tggcgacccc atacgcgcgc
      361 cgcatgaccg ggcagacgct ttatgtcgac ggtggcgtga gtatcatggc ctgattatgg
      421 agcgcgctaa cccgccagtt ccgggaaatc cggaatccgc cgccagcgaa ttcccaccgg
      481 tgcgcttgac gctccgcgac gggcgcagcg tcacgctacg cgcagtccgt ccggacgatg
      541 ccgacgccat gcgagctgcg ctcgacggcc tgtccgcgga ggcgcgctac agccgcttca
      601 tgggggcgat caaggttacg cccgccttgg tcgagcgcgc cgtccgaccg ccggcggatc
      661 gcgaccgagc attggtggcg gttgcgggcg agggcaccga cgcgacgatc gtcggcggcg
      721 cccgctacat cggtggagtg gacggcgcaa cctgcgagtt cgcggtcacg atcacggatg
      781 gttggcgcgg gtcgggactc gcctccagca tgatgagagc gctcatccgg gacgccgccg
      841 cgcacgggct gaagcgcatt gaaggcttcg tgctcgcccg gaacacgccc atgttaaacc
      901 tcgcccggcg cttaggcttc caagtggctt cgagcgagga ggggccgacc gtcaagctgg
      961 tgcggctcga ccttgagtcg gattgaccat gcaggccgcg gagcttgctt tttcccggtg
     1021 cgggacacgc ttgcagtctc agtatcgcgg ttgttgggcg tcaaaagccc tttcataaac
     1081 tgccatctaa aggagaagct caatgagcga cacatcagaa gacctgggtc tcgttactgt
     1141 gctcttgcaa aggctgcagc agcagaggct tccgagagca cttgcgttga aggaaaaagt
     1201 tgatcgcggc gagcggcttg aagattttga tatagcgttc ctcgagggag tgtttgccga
     1261 tgccaatgag attaaaccac ttgtggaaag acaccctgaa caccaggcat tggtgtcgag
     1321 cgtagtgaat ctttacaaag agattacgaa tagggctctt gagaacgaaa aggggtcgta
     1381 agcgcctagc gaatcggcgt tgctgcgcca gcagcgtctg ttctagccgg tagagcgctg
     1441 ctcattcaga cgcggtgcag gttgagcacg aggaggactg atcatgaaaa tgatagttag
     1501 cgtcttcacg cagtgcgcga ccgcgcgaga gctggtgaaa gcctgcttcg cctgtaatta
     1561 ataagacgag cggtttcaac catccgatag ggacaccaag agggggccca ggcatgtttc
     1621 agctgttcca atctatcttc ggcccgagcc cccccgaaca ggggcgttat ccccggcagc
     1681 ttgtcgaagc ggccatcgag cgcgcgctcg acgccaccga tccgcgtctg cgcttcctct
     1741 ccggctacca gaagaagctg cggcccgccg tgatccacgc catcgatcaa gtgatgagtc
     1801 tggtggatgg actgccgccg ccggtcgagg tgagccgcgc ggtctttagg gccgactcgc
     1861 ggctgccggc cattttcgtt tcggccgacc acctacaaca ggtggtcagc gcagaccgtg
     1921 ccctagccga ctttctcgcg cggccaggcg gtctcgccac ggagcgggtc gtggccgtgc
     1981 tcctcatgca gaaagaggag cggaatgttc tcgggatgga gctccaaggc gaaatggtga
     2041 tgcgggaggt tccccaggtg accgtcagct tcaccggaca ccgcctcctc gatcccacag
     2101 ccgcggagga ggagacgcgc cggctgctca agcgccgcgc ctttgaccac ctgctcgccc
     2161 tcgccctggg acggatcgcc tcggcgcacg cggaacgggt cgagctggaa gggcagcgca
     2221 ccctgctgcg ccacaagctt gctacgctcg aatccgggcg ctggagcttc gagccagccg
     2281 gagctggtga cactaccaag gtggccgcga tcgaggcgca gctgggcgag atcgagggcc
     2341 agctgcggga gctcggggcg gacgccggaa cgctgcggct ccatctggat atcctgattg
     2401 aagtgctcgg tcgggcgcag gagcaacttt ggtcgaaacg aatcagcctt atcgtggatc
     2461 ggatgggcat caagcgtaag cgagcgacag ataccgctcc ggagattgtg ctcgatgagc
     2521 tgcacagcgc cagtgggcgc acggccatca tgctgccggt gagtattccg cccggcgagc
     2581 tgagaccgcg cagcgacctc cttgccgaag cgcagcgcta ccttggctta tgaacctaac
     2641 ccggccaagc cggaaccaaa aaggtcaatg caaagcatgg cctcgacctg atcgggggac
     2701 acgcgagagc cgtgacctcc gctttgcctt ccacgagcgc gatcccaaac agcacggtgt
     2761 gttcggtcat gttggcggtg agcacccggc tcatctgcag gtagctcagc gtgttgatcg
     2821 agctcgccgc gagcacgaaa agcaacgtgt tgcagagcgg gtcgtccggc gccgtggcgt
     2881 attagcgccc ggccttgatc tcgctcgtga gcggcttgcc cgcggcgaac tcgctcaggt
     2941 ttgcgatcgt tgtctcgcaa atcgttgtca ccgcctcgcg cgtgaagaac gcttggtggc
     3001 cggtgactat cacgttcggg aacgccatca gccgctggat cacgtcgtcc gtgatcacct
     3061 cgctcgacag gtcctggaag aagatgtgcg cctcctgctc gtagacgtcg agcccgagcc
     3121 cgccgagccg gcctgacttg atcgcctcga tcgccgcctc ggcgtccacc agcgcaccgc
     3181 ggctggtatt gatcaggatc gcgccgcgct tcaagcgcgc gagggtctgc gcgttgatga
     3241 tgtggtgcgt ctcgggcgta agcgggcagt gaagcgagat cacgtccgcg cgctcgccga
     3301 tctccttccg gtcgcggtag cgaccgccga gcgcctcgaa ctccgtgctc gggtacttgt
     3361 cgtagccgag cacctcgcag ccgaagcccc gcatgatccg cgcgaacacc cgcccgatct
     3421 tgccggtgcc gacgacggcc accaccttgc cgttcaggtc gaagccgatc aagccgtcga
     3481 gcgcgaagtt gccgtcacgc gtgcggttat aggcgcggtg caccttccgg ttcactgcca
     3541 gcaacagcgc gaccgcgtgc tcggcgacgg agtatggtga gtaatccgtg acgcgggcag
     3601 ccttgatccc cagcgcggcg gctgctttga ggtcgatatg gttgaagccc gtggagcgcg
     3661 tcgtgacgaa ccgcgtgccg ccgccagcga gcgtccgcag ggcggctgca tcggctttgt
     3721 cgtttacaaa aatgcaaact gcctggcagc ccgcggcgag gggcgcggtc tccggctcga
     3781 ggagccggtc gaggtagacc agctcatgac cgtggctggc gttcgcggcg tcgaggaact
     3841 cacgttcgta gcgcttggcg ctaaacacgg cagttttcat cacggggtcc ttgcggggag
     3901 ggggcgagtc tcgactcaat gctagcagac tcacgcggcc aatgtctatt gggctaatat
     3961 tgggctaaag tccaatagaa accgcgttgt caggcgtgta cagtaatttc gagtggcggg
     4021 aaggcgcgcc gccgcggatc cccgtggcgg tgaagctcgc gcccgccttc accgctgtcg
     4081 gcagtgccgg ggcggtcatg acgacgtccg cgccggcgag cagatacttg acgatctgct
     4141 catgggcccg catatgcgca cgctcaaggc aggcttgacc gctggctcga ggagcagcgg
     4201 ttcgagtcgg tggccgcggt gcgcggcata aaggacagta tgtcgaaaac aacgacgccc
     4261 tcacggagta cgtcccgcag aaactggtca cctaacccct agaacggaga accagcatgg
     4321 aatggctcaa ggcgctgctc gagtcgcaac cgctgttcgc gttattcctc accatcgcgc
     4381 tcggctactt gcttggcgag gttaacatca agggtctctc gctcgggtcc ggcgcggtgc
     4441 tcttcgtggc cctcgcaatc ggcgcgatcg cgccgaaatc ggcgccgccc gcgctgctcg
     4501 gcacgctcgg actgctcctt tttctgtacg gcgtcggcct gggttatggc aaggagttct
     4561 tccgggggtt gtcaagctcc caggggctga aggcgaactt cgcggcgctg tgcggcctcg
     4621 cgggggcgct cgcggtcacg atgatcgccg tggagctggc aggggtgaag ctcggttacg
     4681 cgctcggcac gtttgcgggc gcaggcacca gcaccgcgac gctgcaagcc gtgatcacag
     4741 cgctgaagag cgacgatccc gcggtcggct acagcgtggc ctacccaatc ggcgtggcgg
     4801 tgccgatcct gatcctgtat ctcggcaacg caattctcaa gcccaagatt ccgcctccgg
     4861 caggctcgag actcgaattc gtcgaggtgg cggtccgcag cgacaaggtc atcggcaagc
     4921 ccttcgcttc ggtcaaggcg aaattccccg ttaacatgga cgtcgggctg atccggcgcg
     4981 agcaccgcaa catcgttccc actgagacga tgtcgctaca ggccgatgac gttttgctgc
     5041 tcatttcgac cgacaaggcg gcgctcgcgc gcgtcgcgca ggaaatcggc gaggtacagg
     5101 ccggacgcgt gaccaatgac cgcaccgacc tcgactacat ccgcgtgttc gcgtccaagc
     5161 gcaccgtcgt cggggtgccg ctgtcgacgc tcaaacttcc aatggagtcc gagtgccggc
     5221 tcctgcacgt gcgccggggc gacgccgatc tgctgccaca cgacgacctc atcctcgagt
     5281 tcggcgaccg cgtcgggttg cttacgcctc gcgagcacat tcccgagttg cgcaggtact
     5341 tcggcgattc catcaagggc acggcggaat tcagctatat ctcgatcggc atcggcgccg
     5401 cgatcggcct ggcgatcggg atgatcccga tccccattcc cggcgtcggc aagctgagcc
     5461 tggggctcgc cggtctgctg ctgttcgcgc tatggctcgg caaggtgcgc cggacggccg
     5521 gggtcacctg gaccatgccg ctgtcggcga acctcgtgct gcggaacttc gggctgaccg
     5581 tgttcctcgc gcaggtcggc atgtcttcgg gccagaagtt cgtgaatacg gtggccgcca
     5641 cgggctggac gttcctcgtg cttagcgtct tgatcgtcac cgtgcttgtc ctcttaacaa
     5701 tgatcgtgtg catggtattc tttaagatgc cgtatgacga ggtcggcggc attgtgtccg
     5761 gagtgactgg caatcccgcc atcctcgcat tcgcggcgcg catcacgcct accgacaagc
     5821 cggacatcgg ctacgccatg atctttccgt ccatgacggt ggtgaagatc atcattgtgc
     5881 aagtggccgc cgtgctggtg ggcgccggct gagacgctca aggcagttat cgcactgtat
     5941 gaactgcgca ttaattcaaa gtcctcgccg gacccccggg acctctgatc gcccactctc
     6001 tcagagaagg ggaaagaaga tggttggctg caccgaggtt ccggccgcga tggctgctga
     6061 tttcgacggt tcaaagctgc tgatctgtgc gccggttgaa gcgatggact gcttcgcgtt
     6121 agcgggagtc gaccacaggc gcttctttta tggagaaaga aaagatggca attcactact
     6181 gcgttctgct tggcgtggtc gcaataatag tcactgcctg cgcaagtcag acggcctcca
     6241 agacaccgcc acagcatcaa ctcgtcgtcg agtctcgaga caagcttccc gcggacatgc
     6301 gccaatgcac ccaacagcat ggctatgatc cggctaaggt agcgggcgtt gcagaaagcg
     6361 cgttggcgcc gcaggaacta ccgtggcggc aatgtgtcta cgacgccctg cgcgtttatg
     6421 ctcgtgc
//
LOCUS       DBNGSB010000020         3691 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2160526, whole genome shotgun sequence.
ACCESSION   DBNGSB010000020 DBNGSB010000000
VERSION     DBNGSB010000020.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2160526"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="AC159B_00550"
     CDS             <1..930
                     /locus_tag="AC159B_00550"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding SpoIIE family protein phosphatase"
                     /protein_id="HIR3376274.1"
                     /translation="IGDVAGHGIAAAAAMAQVRHLVRALAIQGRSPADVMAAVNHYLL
                     QLPDSTHLSMWLAMLDPITGDLIYGGAGHPPVLVQGAYGVRELTSQSPPIGFSPSITY
                     REERAQLAPGERLIAYTDGLIEVTRDPIEGERRLREAVSATFGQPRDLTVRSLVTDVL
                     RDDHQGDDVALLLVDVLPSSAPLSFALPAAPENLRRVRHGVRIYAERMGFPPRRVEEI
                     VAAVGEAALNIVEHAYRGETGEVAVDAIRRDDTFTVSVRDSGEWRRVLERGRGRGRRL
                     MESFADSVETVAGPAGTKVELRWTLPKTEALRE"
     gene            complement(940..1707)
                     /locus_tag="AC159B_00555"
     CDS             complement(940..1707)
                     /locus_tag="AC159B_00555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HIR3376275.1"
                     /translation="MAARQQRKRADRDAIRALFQEHWKTKDRAIREQLVLAHSSLASY
                     LARKFSNRGEPLDDLGQVAQIGLLKAIDRFDPTRGIEFTTYATVTIVGEIKRHFRDKY
                     WSIRVPRRLRELNNSLMKTVEVMAQRMGRSPTLPEIAEETRVPFEEVVEAFELGRAYN
                     PASLDAEVAEGDDEHASSLLDYLGQDDPAINRLEDHQTVQGALASLPDRHRDILKMRY
                     YEGLSQAEIARRLGISQMHVSRIQRDALKKLRAMIEA"
     gene            complement(1737..2141)
                     /locus_tag="AC159B_00560"
     CDS             complement(1737..2141)
                     /locus_tag="AC159B_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3376276.1"
                     /translation="MPVEQQSQEPDTSPAERIELTVPGRPEYVVVVRLAAAAVAGRMP
                     FSFDDIEDLKVAVGEVATAAILSGAPKIAVWFDVGAQRLEIRVVHRPTRVDRPTQERE
                     LDKLLVQVLMDEVRTDQDGADHVTHMVKNVPG"
     gene            complement(2219..>3691)
                     /locus_tag="AC159B_00565"
     CDS             complement(2219..>3691)
                     /locus_tag="AC159B_00565"
                     /inference="COORDINATES: protein motif:HMM:NF015557.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR3376277.1"
                     /translation="VAGLSAFISNTAATAFFLPVAIGAALRTKISPSQILMPLAFASI
                     LTSSVTLVSTSTNIVVSGVITNYKQPPIGMFELAPVGIPIAILGLAYMWVIGRRLIPP
                     RAAAADLTEEFGLGSYLTEVLILPDSKLVGKTLAESALGRDLDLTVMQIVRDKTRTLT
                     PRASTKLEAGDVLLVEGRRDEILKIKDTAGIEIKADVTLSFPEVQSEDTRLAEVVLMP
                     RSPLIGRTLKGVEFRERYGLQVLAINRAGASIRSKISQVRLRMGDVLLIQGHREHLAA
                     LDQDDTVRILGALQAERPKLRRAPVAILIFIGALGAATVGLMALPVAVLLGAVLVFLT
                     RCLTPEEAYRVVEWKALVLIGSMLALGAAMEGTGTAKFLAARIVDVAGDTRPLWLLSG
                     FFALTVLLTQPMSNQAAAVVVVPVAVQMAMQLDLNPRTFAMMIAIAASTSYMTPLEPS
                     CLMVYGPGRYRFSDFLKVGALLTLLIYGVAIFLVPRVWPL"
BASE COUNT          685 a         1164 c         1155 g          687 t
ORIGIN      
        1 atcggcgacg tggccggaca tggtatcgct gccgccgcgg ccatggctca ggtccggcat
       61 ctggtccggg cgctggccat ccaaggccgg agccccgcgg atgtgatggc agccgtgaat
      121 cactacctgc tgcagctccc tgattcgacg catctctcga tgtggctcgc gatgctcgac
      181 ccgatcacgg gcgatctgat ctacggaggc gcgggccacc cgccggtgct tgtgcagggt
      241 gcgtatgggg tgcgcgaact gaccagtcaa tcgccgccga tcggattctc cccgtccatt
      301 acgtatcggg aggaacgagc gcagctcgct cccggagaac gcctcatcgc gtacactgac
      361 gggctcattg aagtgacccg ggatccgatc gagggtgagc ggcggctgag agaagccgtc
      421 tccgctacgt tcgggcagcc ccgcgatctc acggtgcgct cattggtcac ggacgtgctg
      481 cgcgacgacc accagggaga cgacgtcgcc ctcctgctgg tcgacgtcct gccatcctct
      541 gcgcccttgt cctttgcgct gccggctgct cctgaaaatc tgcgccgtgt ccgccacggg
      601 gtgcggatct acgccgagcg tatggggttc ccaccccggc gggtcgagga gatcgtcgcg
      661 gccgtcgggg aggctgcgct caacatcgtc gagcacgctt accggggtga gaccggcgag
      721 gtggccgtgg acgcaatccg acgcgatgac acgttcaccg tctcggtccg tgacagcggg
      781 gagtggcgac gtgtgctgga gcgcggccgc ggacgcggcc gcaggctcat ggagagcttc
      841 gccgacagcg tggagaccgt cgccggacct gcgggaacga aggtcgaatt acggtggaca
      901 ctgccgaaaa cagaagcgct tcgtgaatag tgaaggtacc taagcttcga tcatcgccct
      961 cagctttttc aaggcatctc gctgtatgcg tgagacgtgc atctgcgaga tcccaagacg
     1021 gcgcgcgatc tcggcctggg agagtccctc gtaataccgc attttgagga tatcacggtg
     1081 ccggtcgggc agtgaggcca gcgcgccttg aaccgtttgg tgatcctcga gacggttgat
     1141 cgcgggatca tcttgtccca gataatccag aagcgaagac gcgtgctcgt catcgccctc
     1201 cgcaacctcg gcatcgaggg acgcggggtt gtaggcgcgc cctaattcaa acgcctcgac
     1261 gacttcttcg aacggcaccc tggtctcttc cgcgatctcc gggagcgtag gcgaacgtcc
     1321 catgcgctgc gccatcactt cgaccgtctt catcagagag ttgttcaact cacgcagacg
     1381 ccgtgggaca cggatcgacc agtatttgtc gcggaaatgg cgcttgatct cgccaacgat
     1441 cgtgacggtt gcgtaggtcg tgaactcgat gccgcgcgtt ggatcgaagc ggtcaatcgc
     1501 cttcaacaat ccgatctgtg caacctggcc gagatcatcg agcggctcac ctcggttgct
     1561 gaatttgcgg gcgagatagc tggccaggct actgtgcgcc agcaccagct gttcacggat
     1621 cgcgcggtct ttcgtcttcc aatgttcctg gaagagcgcg cgaatggcgt ctcgatctgc
     1681 ccgttttctt tgctgcctcg ccgccatcgt tgagtgtccg ccccggctat cctcgactac
     1741 ccaggcacat ttttgaccat gtgagtgacg tgatcggccc catcctggtc ggtgcggacc
     1801 tcgtccatca gcacctgaac gagtaacttg tccaactcgc gctcctgggt ggggcgatct
     1861 acgcgggtgg ggcggtgcac gacgcggatc tccaggcgct gcgcgccgac gtcgaaccac
     1921 acggcaatct tcggggcgcc ggagaggatc gcggccgtgg ccacttcgcc gacagcgact
     1981 ttcagatctt caatgtcatc gaaggaaaag ggcatacgac cggcgaccgc tgcggcagcg
     2041 agcctgacga caacgacgta ttccggccgg cctggaacgg tgagctcgat tcgctccgca
     2101 ggtgaggtgt ccggctcttg ggactgttgc tccacaggca tcacccttct cgaagaggat
     2161 ttgacgcagt ttcccatacc gtcctttgcc cgatttctcc ccttttcctc cggctgaatc
     2221 ataacggcca cacccgtggc acaagaaaga tggccacgcc gtaaatgagc agtgtcaata
     2281 aggcccccac tttcaagaaa tctgaaaacc ggtaccgccc cggtccgtac accatcaggc
     2341 aggaaggttc cagcggggtc atgtacgatg tgctggccgc gatggcgatc atcatcgcaa
     2401 acgtcctcgg gttcaaatcc agctgcatgg ccatctggac cgcgaccggg acgacgacca
     2461 ctgccgccgc ctggttggac atgggctgcg tgagcaggac tgtgagggcg aagaatcccg
     2521 acagcagcca gagaggccgg gtgtcacctg cgacgtcgac gatgcgcgcg gcgagaaact
     2581 tcgccgtgcc ggtgccctcc atcgctgcgc cgagcgcgag catgctgccg atcaacacaa
     2641 gcgctttcca ctcgactacg cgatacgcct cctcgggggt aaggcagcgc gtgaggaaca
     2701 cgagcacggc gccgagcaaa accgccacgg gcaacgccat caggccgacc gtcgccgctc
     2761 caagcgcgcc gatgaagatg agaattgcca cgggcgctcg gcgcagcttg ggccgctctg
     2821 cctggagcgc gcccaagatt cggaccgtat catcctggtc gagcgcagcc aggtgctcgc
     2881 ggtgcccctg aatgagcaac acgtcgccca ttcgcagtcg cacttggcta atcttgctcc
     2941 ggatggacgc tccggcgcgg ttgatcgcca ggacctggag cccatagcgc tcgcgaaact
     3001 ccaccccctt gagcgtacgc ccgatcaacg gggagcgggg catcagcaca acctcggcga
     3061 gccgtgtgtc ctccgattgc acctccggga acgacagcgt cacgtcggcc ttgatctcga
     3121 tgccggcagt gtctttgatt ttgagaattt catcgcgccg tccctcgacg aggagcacgt
     3181 cgccggcctc gagcttcgtc gaggcgcggg gcgtgagcgt acgagtcttg tcacggacga
     3241 tctgcatcac ggtaagatcg agatctcggc ccagggccga ttccgcgagc gtttttccta
     3301 ccagctttga atcgggcagg atcaatacct cagtcaggta tgaccccagt ccaaattcct
     3361 ccgtcaggtc ggcggccgct gcgcggggcg gtatgagccg gcgcccaatc acccacatat
     3421 atgcaaggcc cagaatcgcg atggggatgc ctacaggcgc gagctcgaac ataccaattg
     3481 gcggctgctt gtaattagtg atcacgccgc tcacgacgat attggtcgag gtgctgacga
     3541 gcgtgaccga gctcgtgagg atcgacgcaa acgcgagcgg cataaggatc tgtgacgggc
     3601 tgatcttggt ccgcagcgcc gcgccgatcg cgaccggaag gaaaaacgcc gtggcggcgg
     3661 tgttgctgat gaatgccgac agccccgcaa c
//
LOCUS       DBNGSB010000021         4372 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2313931, whole genome shotgun sequence.
ACCESSION   DBNGSB010000021 DBNGSB010000000
VERSION     DBNGSB010000021.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2313931"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="AC159B_00570"
     CDS             complement(<1..438)
                     /locus_tag="AC159B_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376278.1"
                     /translation="MQRRLQVIILAHVWALVPVAVAWFVRPAWRGEYAPADVSALLPL
                     TIISAAYLILRTWLTVIRWIPRLVALYPYVDVALVTAGLIILRNPSDPLSILYFIPLA
                     SAVASLSVGQITAVGAATVIAYLVAIIASSVPWTIGMLYRLIML"
     gene            561..1538
                     /locus_tag="AC159B_00575"
     CDS             561..1538
                     /locus_tag="AC159B_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HIR3376279.1"
                     /translation="MLLLREADVRGLLSMEELIRLMGDALRAFSTGGVLQPVRLALQI
                     EPHAGYLGVMPAFIQTNGALAAKLVTFYPKNAENRRPTHLAVILLWDSRTGDLLSMMD
                     GRLITEMRTAATSAAATNVLARSDAGTLAILGSGVQARSHLEALRLVRTIDAVRVWSR
                     TPAHAREFAREMHAELGMVVEQTATGDGAVRGADLIVTATSATSPVLRGEWISKGAHI
                     NAVGAPRPTWRELDTEAVTRSRVFVDSRAGALTEAGDLIMPIKEEVIKESHIAGEIGE
                     VLAGQVEGRRGADDITLFKSLGMAVEDVATAHYLYEKAREREIGEEVTV"
     gene            1538..2686
                     /locus_tag="AC159B_00580"
     CDS             1538..2686
                     /locus_tag="AC159B_00580"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003954 - NADH dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006116 - obsolete NADH oxidation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3376280.1"
                     /translation="MAGAKLVILGGGFGGITAALALRKALDGNHAITLVDRRPTFMMG
                     LRKLWVLAGRGTIGDGTRSLSALREKGIDLRQGTVTQIDPAARRVTTDAGALPYDYLV
                     IALGAESRPDLVPGFSSSVFDLYDAVDVERLAPRVKSFTAGRVVVGILGVPYKCPPAP
                     YEAALLLHDVFEQRGVRRAIQIAAFTPQPMSLPVVGTAGCAQVEGRLAASGITFMPGR
                     KVDRLEASTAITEKGSIAADLFIAVPPHRPPAVIKNSGLQMTGEWLFADPATMRTSDP
                     RVYGVGDVIEIPLANKMSLPKAGIFAESQAKVAASHIASDIAGSRSPASFDGWGFCFI
                     EIGNGQAAKVQGHFTAPNGPQVEITAPSEAALKEKVEFERSRLTNWFS"
     gene            2814..3272
                     /gene="mraZ"
                     /locus_tag="AC159B_00585"
     CDS             2814..3272
                     /gene="mraZ"
                     /locus_tag="AC159B_00585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HIR3376281.1"
                     /translation="MTSGAKWGPVGSMFKGEHHYSLDEKGRIVVPPTFRRALGSAVVV
                     TRGLDECVAVYAPAEWAKNERKLRSQAVSRRDIVRFMLASAEDADIDGQGRLTLPAHL
                     RQYAKIDREAVVIGVGARLEIWSLENWQKYIERVQSKASELASELQDLSL"
     gene            3274..4224
                     /gene="rsmH"
                     /locus_tag="AC159B_00590"
     CDS             3274..4224
                     /gene="rsmH"
                     /locus_tag="AC159B_00590"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIR3376282.1"
                     /translation="MGHVPVLLEETIRWLDPKPGGVYVDATLGGGGHAEAILERIAPD
                     GRLIGMDRDDDAAAEASQRLQRFGEAAVIVRENFTAIKEVLHSLGLAQVDGILFDLGA
                     SSMQFEDADRGFSFAAAGPLDMRMDRRQHLTAGELINRRPEEELAGLIWRYGEERWSR
                     RIARAIVRARPLSTTTALAEAVAGAIPRRSWPRGIHPATRTFQALRIAVNDELLNLEN
                     AIPVAAEALREAGRLCAITFHSLEDRTVKHTFLRLSRGCTSPPGTSASNSHERRRLRI
                     LTKKPITPSAEEVARNPRARSAKLRVAERLATDLKSYKDR"
BASE COUNT          891 a         1256 c         1465 g          760 t
ORIGIN      
        1 gagcatgatg aggcggtaga gcatgccaat ggtccacggc acgctggagg cgataatggc
       61 gactagatat gcgatgacgg tcgccgcgcc gacggccgta atctgtccca ccgagagtga
      121 ggccacggcg gaagcgaggg ggatgaagta gaggatcgag agtggatcgg aaggattgcg
      181 caaaatgatg agccctgccg tcacgagcgc gacatccacg tatgggtaga gggccaccag
      241 ccgcgggatc catcgtataa ccgtgagcca ggtacgcagg atcaggtacg cggcgctgat
      301 gatggtgaga gggaggagcg cgctcacatc cgccggggca tattctccac gccaggccgg
      361 ccggacgaac caggccacag cgaccgggac cagcgcccag acgtgggcga ggatgatgac
      421 ctgaaggcgc cgctgcacag cgtgaactag ttctatgtct tcccggtcta ccccgtctcc
      481 ccagtcagcc cagtctaccc agtctcaagc aactgcaatt agacggggaa gacagggaag
      541 atggggaaga caaggtagac atgctgttgc tccgtgaggc ggatgtgcgc gggctactct
      601 ctatggagga attgatccga ttgatggggg atgcgctgcg ggcgttctca acgggaggcg
      661 tgctgcagcc ggtacgcctg gcgctgcaga ttgagcctca cgcgggatat ctcggcgtga
      721 tgcccgcgtt cattcagacg aatggcgcgc tcgccgcaaa gctggtgacg ttttacccga
      781 agaacgcgga gaaccgtcgg ccgacccacc tggccgtgat tctattgtgg gacagcagga
      841 cgggagactt gctctccatg atggacggtc gcctgatcac cgagatgcga accgccgcga
      901 catccgcggc ggctacgaac gtactcgcgc ggagcgatgc aggtacgctg gccatcctcg
      961 ggtcgggcgt gcaggcgcgc agccacctcg aggcactccg actggtccga accatcgatg
     1021 ctgtacgtgt gtggtcacga acgcctgccc acgcgcgaga attcgcccgg gaaatgcatg
     1081 cagagcttgg catggtcgtc gagcagacgg cgaccgggga tggagccgtg cgaggcgccg
     1141 acttgatcgt caccgcaacc tcggcgacat ctcccgtgct gcggggcgag tggatctcaa
     1201 aaggcgctca tattaatgcc gtgggcgcgc cgcgcccgac gtggcgagaa ctcgatacgg
     1261 aggcggtgac ccggtcccgc gtgtttgtcg actcacgcgc cggtgcactc accgaggcgg
     1321 gggacttgat catgcctata aaggaagaag tgatcaaaga gtcgcacatc gccggcgaga
     1381 tcggtgaggt gctggcgggc caagtagagg gacggcgggg agccgacgac atcaccctct
     1441 tcaagtcgtt ggggatggcg gtcgaggacg tcgcgaccgc ccactatttg tacgagaaag
     1501 cgcgcgagcg tgagatcgga gaggaggtga cggtctgatg gcgggcgcaa agctcgtgat
     1561 cctgggcgga gggtttggtg gcattacagc ggcgctggcg ttgcgaaagg ccctcgatgg
     1621 taaccatgcc atcaccttgg tcgatcgtcg cccgaccttc atgatgggcc tgcgcaagtt
     1681 gtgggtactt gccggccgcg ggaccatcgg cgacggcaca cgctcgttgt ccgcactcag
     1741 ggagaaggga attgacttgc gtcagggcac cgtgacgcaa atcgacccag cggcgcgcag
     1801 ggttacgacc gatgcgggag cactgccgta tgactacctt gtgatcgcgc tgggcgcgga
     1861 aagccgcccg gatctcgtac cgggattttc aagttcggtc tttgatctct acgacgcggt
     1921 agacgttgaa aggctggcac cgcgcgtcaa atcattcacg gccggtcgcg tcgttgtcgg
     1981 gatcctcggg gttccctaca agtgtccgcc ggctccttat gaggctgcgc tgctgcttca
     2041 tgacgtgttc gagcaacggg gggtccgccg ggccatccag atcgcggcgt ttacgccgca
     2101 accaatgtcg ctgcccgtcg tgggcacggc ggggtgtgcg caggtcgagg ggcgactggc
     2161 ggccagcggc atcacgttca tgccgggccg aaaggtcgac cggctcgagg cgagcaccgc
     2221 cattaccgag aagggaagca tagccgccga cctgttcatc gccgttcccc cgcaccgtcc
     2281 gccggccgtg atcaagaaca gcggccttca gatgaccgga gaatggttgt tcgcggaccc
     2341 tgccacgatg cgaacttcag atccacgcgt gtacggagtt ggcgatgtca tcgagatccc
     2401 gctcgcgaat aagatgtcgc ttccgaaggc gggaatcttc gccgaatcgc aagcgaaggt
     2461 ggcggcgtca cacatcgcct cggacattgc gggcagtcgc tcgccggcat cattcgacgg
     2521 gtggggattc tgcttcattg aaatagggaa cggacaggcg gccaaagtgc agggacattt
     2581 cactgccccg aatggaccgc aggtcgagat cacagcgcca tcagaggcag cgttgaagga
     2641 aaaagtcgag tttgaacgga gccggttgac gaactggttt tcctgatcgt ggttgacgtc
     2701 cacatatagc gccttcggcg cactgatgcc ccaccatatt gcggttgggg ctttttttgt
     2761 ggggattttt ctgaattctg gtctataaag aggagttctg tggaacgagt tgaatgacta
     2821 gtggagcaaa gtggggacca gtggggagca tgttcaaagg agagcatcac tactccctgg
     2881 acgagaaggg gcggatcgtc gtgccgccca cattccggcg ggcacttggg agcgccgtcg
     2941 tggtgacgcg tggtctcgat gaatgcgtcg cagtgtacgc gcctgccgaa tgggccaaga
     3001 acgagaggaa actgcggtcg caggcggtca gccggcgcga catcgtgcgc ttcatgctgg
     3061 ccagcgcgga agacgccgat atcgacgggc aggggaggtt aacgctcccg gctcatctcc
     3121 ggcagtacgc gaagatcgat cgcgaggcgg ttgtgatcgg tgtcggggcc aggctcgaga
     3181 tctggagcct ggagaactgg cagaagtaca tcgagcgagt gcagtccaag gcgtcagaac
     3241 tggcaagcga gttgcaagat ttgagtttgt gacatcggcc acgtaccggt tctgcttgaa
     3301 gaaacgatac gctggctgga cccgaagccc ggtggcgtgt atgttgatgc gacgctcggc
     3361 ggcgggggac acgccgaggc gattctggag cgaatcgcac ccgacgggcg gttgatcggt
     3421 atggatcggg acgacgacgc agcagccgaa gcgtcccaga ggctccagcg attcggcgag
     3481 gcggcagtga tcgtgcgtga aaacttcacg gcgatcaaag aggtcctcca ttctctgggc
     3541 ctcgcacagg tcgacggcat cctcttcgac ctgggcgcat cgtcaatgca attcgaagac
     3601 gcggatcgcg ggttctcgtt tgcggccgcg ggcccgctgg acatgcggat ggaccgccgg
     3661 cagcatttaa cggccggcga gctgatcaac agacgaccag aggaggaatt ggcaggactc
     3721 atctggcgct acggtgagga acggtggtcc cggcggattg cgcgcgcgat cgttcgggcg
     3781 cgaccgctgt cgaccacgac ggcactggcc gaggccgtcg ccggcgcaat cccgcgacgg
     3841 tcctggcccc gggggatcca ccccgcgacg cgtacgtttc aagcgctacg gatcgcagta
     3901 aacgacgaac tactgaacct tgaaaacgcc ataccagttg cggcggaggc tctccgggag
     3961 gcaggtcgac tctgcgccat taccttccac tccctggagg atcgaacggt caaacacacc
     4021 ttcctgcgcc tctcccgtgg gtgcacctcg ccacccggga cctccgccag caactctcac
     4081 gaaagacggc ggctgcgaat tctcaccaag aaaccgatca cgccgtctgc tgaagaagtc
     4141 gcaagaaatc cccgcgcgcg aagcgcgaag ctgcgagtcg ccgagcgcct cgcaacagac
     4201 ttgaaaagct acaaagacag gtgagaacaa gacaaattgg tttcacgaac taaatagggc
     4261 tggggcggga tcgtcgctga ccttcggtct tcgcaccttc actcataccc cgaagaaatc
     4321 ccctacacac cacatcagag cacctccggc aaccggggtc cggcgtcccg cc
//
LOCUS       DBNGSB010000022         6607 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_1241457, whole genome shotgun sequence.
ACCESSION   DBNGSB010000022 DBNGSB010000000
VERSION     DBNGSB010000022.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 6607)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6607)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6607
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_1241457"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            359..1513
                     /locus_tag="AC159B_00595"
     CDS             359..1513
                     /locus_tag="AC159B_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose 1,6-bisphosphatase"
                     /protein_id="HIR3376283.1"
                     /translation="MRVTISVIKADIGAIGGHTMPSTEILGIVRDRVERERKKLLIDA
                     FVCHTGDDIAMIMTHAKGQDSPDVHQLAWDALLKATDKAKSQGLYGAGQDLLKEAFSG
                     NVRGLGPGSAEIELDERPSEAFMVTLGDKCGPGAFNLPLYLAFADPMYSPGLMLSEGL
                     HDGFTFHIMDMDHTEGDRLIVLEAPERLYDIASLLRDTNRYAIEAIWSRKYPNEQAAA
                     IGTTRLRNIAGRYVGKDDPPAVIRVQKIFPATEEFGPAFAVAPFVGGDTRGSHNLPLM
                     PVPMNTPASTFFCCPMVSALGFSMHDGILTAPMDLFADPFWNQVRDRVAEKAIEMRRQ
                     GFFEPAMLPMSELEYGGIVARLRKLDPEFAVRNGRGTTATAQAVTADDPD"
     gene            complement(1638..1787)
                     /locus_tag="AC159B_00600"
     CDS             complement(1638..1787)
                     /locus_tag="AC159B_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376284.1"
                     /translation="MSELQVVYLFLALLGLFIVIGALIPKYLSSLVRYVDETVLKENK
                     GREHH"
     gene            complement(1829..3154)
                     /locus_tag="AC159B_00605"
     CDS             complement(1829..3154)
                     /locus_tag="AC159B_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0052929 - ATP:3'-cytidine-cytidine-tRNA
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HIR3376285.1"
                     /translation="MGTLRDGAVDVVRRLRDAGCTAFWVGGCVRDLEMGREPHDYDIV
                     TDARPERVTSLLDRAVLVGAEFGVVAVSSHGHQYEVATFRTEGPYLDGRRPSSVKFAT
                     AEDDVRRRDFTINGLLHDPLSGKTSDYVDGRQDIRRRIIRTIGTPQERFAEDYLRMLR
                     AVRLAAELDFTIEAETFRAIAALAPSVERVSAERIRDELLRLLVAPGRSNGVRLLDES
                     GLLRIILPEVTATKGVEQPPEFHPEGDVFTHTLLALKYLRNPSPELALATLLHDVGKP
                     PTMTRVDRIRFNNHDDVGAQMAETICRRLRCSNEQVDRVVSLVQNHLRMKDLPKMRQA
                     KAARFLLQPDAADHLELHRVDCQASHGDLSVWEWAVKTQQELAATKPLQPRLLSGDDL
                     IQLGFPPGPKFKEILDYVEDAQLEGQVHSYEEAMALVRRVFPAEPAVED"
     gene            complement(3179..4504)
                     /locus_tag="AC159B_00610"
     CDS             complement(3179..4504)
                     /locus_tag="AC159B_00610"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR3376286.1"
                     /translation="MKPVRLALLAWVVVLVALLLAPPQLAAQSPDEAITALVQEVFTR
                     LRDDALAASDGPSLLRAAVAGVQQVLLGATEVPPVPVLSGTEDEDLRAVAVYVEAAVR
                     SAPGRGELILAAVLRAMVRTVPDPVSAVFTPTELGRYMADLRGEHGTIGLQVDTVGTA
                     INVVEVVEGGPAVRAGIRVGDQIQEIDGLSMEGRSPDTVLALLRGRLGSTVLVSVQRA
                     SSQPQRFSVIREQVREIPVRARMLEPRVGYLRLLGFTERAHEDLERWLLRLDGQDARG
                     LVLDLRENGGGLVEEAVNVASAFLSQGVVAVEEGRRGPITLIVRPAAHRFAGPVVVLV
                     NRGTASASEIVAGALQDAGVSLVGARTFGKGTVQTVYFLQAGWGLRITTSRYKTRAGR
                     AIEGTGLTPDVPVVQTVNQVPFPGDTQLEIARVLVRRRLESTGQRAGQR"
     gene            complement(4533..5645)
                     /gene="mnmA"
                     /locus_tag="AC159B_00615"
     CDS             complement(4533..5645)
                     /gene="mnmA"
                     /locus_tag="AC159B_00615"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HIR3376287.1"
                     /translation="MGKWAVAMSGGVDSSVAAALLAEEGHEVVGLTMNLWPDWLPEPD
                     DSFKACCGVSAIDDARATARQLGIRHYVLNMREEFERAVIGYFVDEYASGRTPNPCIA
                     CNQSIKFSLLLEKVSALGLDGVATGHYARLRHDEKSGRWLVLRAADPHKDQSYVLYGL
                     TQAQLGRVRFPVGGYAKEETRSLARRFGLPVADKPDSQEICFVPRGTYTDVVARFRSE
                     ALAPGPMVDTSGRVIGQHQGIARYTIGQRRGLGIADGEPLYVVGIDRERNAVIVGREE
                     DLLCVELVASRMNWIAFPELRGALLATARVRHAARDVEAVVRPGRASDEAIVHFAEAQ
                     RAAAPGQAVTLYERDVVLGGGIIMNVRTAASLAASA"
     gene            complement(5722..>6607)
                     /locus_tag="AC159B_00620"
     CDS             complement(5722..>6607)
                     /locus_tag="AC159B_00620"
                     /inference="COORDINATES: protein motif:HMM:NF046269.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MGH1-like glycoside hydrolase domain-containing
                     protein"
                     /protein_id="HIR3376288.1"
                     /translation="RIYDALSESLLYFSRHRTTGNSPLIVLVHPWESGLDNSPRWDAA
                     VAKGVRPSRPYRRVDTTKVAAAERPTSRDYDLYMFLVELIATHDYEMRNYLGGTPFAV
                     YDAMFNAVWFRAAKDLNRIALAIDRPPVVSKSSLDAFGRAYHAALWDAEGVMFLDFDL
                     TSQAKIPVHTWAGLAAIYAGLVERAQAEKIYDAYANRCRNCRQLPSVLPDQSGFESDR
                     YHRGPVWVNANWMLARGLYDLGMRSPARRLAEATVELVRSSGFHEYFDAYTGRGAGAD
                     QFSWTAALTIDLLKRPIG"
BASE COUNT         1291 a         2116 c         1968 g         1232 t
ORIGIN      
        1 tcaccgccgt cgtcgtcttc gcgcagccgc ccttttggtt gactagcgcg atgaccctcg
       61 cggattcagg gtagatcatc ggatgctcga ttcatcactg cgttgatacc cagccgggaa
      121 ccgcccgctg ggcgtcgcgc tgaataaagt cgtcctgatt ccgttcggtt gcgtgttcga
      181 ctcttttgaa ggttttcctc ttttccctac agaaagtgct acaaagagca caccgagcgt
      241 gaagctgcta ccaaacagcg ccaaccgtct tgactttcga ccggcaccgt tcctgtgccg
      301 gtcgattcgt cgtttgatag tacatgtcaa tttggtagaa ctttaagggg gcgaagctat
      361 gcgcgtcact attagcgtga tcaaggccga catcggtgcg atcggcggcc acacgatgcc
      421 cagcaccgag atcctgggga tcgttagaga ccgcgtcgag cgggagcgca agaaactcct
      481 gatcgacgcc tttgtgtgtc acaccggcga cgacatcgcc atgatcatga cgcacgctaa
      541 agggcaagac agccccgacg tccaccagct ggcgtgggat gcgttgctca aggcgaccga
      601 taaggcgaaa agccagggtc tttacggcgc cggacaagac ctccttaagg aggcgttctc
      661 gggtaacgtt cgaggactcg gacctgggag tgccgagatt gagctggacg agcggccgag
      721 cgaagcgttc atggtgactc tcggcgacaa atgtggcccc ggcgcgttca acctgccgct
      781 gtaccttgcc ttcgccgacc cgatgtactc gcccggtcta atgctctccg aaggtctgca
      841 cgacgggttc acgttccaca tcatggacat ggaccacact gagggcgacc gcttgattgt
      901 gctggaggcg ccggagcggt tgtacgacat cgcctcgctc ctgcgtgaca cgaaccgcta
      961 tgcgatcgag gccatctggt ctcggaagta tcccaacgag caggctgcgg cgatcggcac
     1021 gacgaggttg cggaatatcg cgggccgtta cgtcggcaag gacgacccgc cggccgtcat
     1081 tcgcgtgcag aagattttcc cggccaccga ggagtttggc ccggcgttcg ccgtggcccc
     1141 gttcgtcggc ggcgacacac gaggcagcca caacctgccc ctgatgccgg tcccgatgaa
     1201 cacgccggcg tcgacgttct tctgctgccc gatggtttca gccctcgggt tctcgatgca
     1261 tgatggcatc ctcacagcgc cgatggacct gtttgctgat ccgttctgga atcaagtccg
     1321 cgaccgggtc gccgagaaag ccatcgagat gcgccgccag ggcttcttcg aaccggcgat
     1381 gctgccgatg tcggagctcg agtacggcgg tattgtggcc aggctgcgca agctcgatcc
     1441 ggaatttgcc gtgcgcaacg ggcgaggtac tacggccacg gcccaagcag tgacggcaga
     1501 tgatcccgac taaaagcaaa actgcgggat aaagggataa agggataaac cgaaaacgca
     1561 cgaggcctgg gggaatgacc ccgggcctca gcatttggaa ctctcttttt cgttcttcct
     1621 ttcccttatc ccgtcttcta gtgatgttcc ctccccttat tctccttaag gacagtttcg
     1681 tcgacatagc gcacgagcga cgaaaggtac ttagggatga gggcgcctat gacgatgaac
     1741 aacccgagga gagcgaggaa gagatagacg acttgtagtt ccgacaccgc gctttcacct
     1801 cgcgttctaa cttccctacc ttcggaaatc agtcctctac cgcaggctcg gccggaaaga
     1861 cccgcctgac gagggccatg gcttcttcgt aactatggac ctgcccctcg agctgcgcat
     1921 cctcgacgta gtcgaggatt tccttgaact ttggaccagg cgggaagccc aactgtatga
     1981 ggtcgtcccc gctcagcagc ctgggttgca gaggctttgt cgcagccagc tcctgctgcg
     2041 ttttgaccgc ccattcccac acgctcagat ccccatggct tgcctggcag tccactctgt
     2101 gcagctcgag atggtcggcg gcgtcgggct gcagcaagaa tctggcggct ttcgcctgcc
     2161 gcatcttcgg aaggtccttc atacgcaggt gattttggac gagcgagacc acgcggtcca
     2221 cctgctcgtt cgagcagcgg agcctccggc agatcgtctc tgccatctgc gcacccacgt
     2281 cgtcgtggtt gttgaaacgg attcggtcca cccgcgtcat ggtcggcggc tttccgacat
     2341 catggagcag cgtggcaagt gcgagttcgg gcgagggatt tcgtaggtac ttcaaagcca
     2401 ggagagtgtg cgtaaagaca tcaccctctg gatggaactc cggcggctgc tccactcctt
     2461 tcgttgcggt aacttcagga agtatgatcc tcaagagccc gctttcatca aggagccgca
     2521 cgccgttgct gcggccgggc gcgacaagca ggcgcaacaa ctcgtcccgg attcgctccg
     2581 cgctgaccct ctcaaccgaa ggagccaggg cggcaatggc gcggaacgtt tccgcttcaa
     2641 tcgtaaaatc gagctcggcg gcgagccgca cagcacggag catccggagg tagtcttcgg
     2701 cgaaccgctc ttgcggggtt ccaatcgtcc gaatgatgcg ccgcctgatg tcctgccgcc
     2761 catcgacata atcgctcgtc ttaccggaga gcggatcgtg gaggagcccg ttgatcgtga
     2821 aatcgcggcg gcggacatca tcctcggccg tggcaaactt gacggacgat gggcgccggc
     2881 cgtccagata gggtccttcg gtccggaatg tcgcgacttc atactggtga ccgtgtgacg
     2941 atacggcgac gacgccgaac tccgcgccca ccaacaccgc gcgatcaagt aacgatgtga
     3001 cccgctcggg acgcgcatct gtgacgatat cgtaatcgtg cggctctcga cccatttcca
     3061 aatcgcgcac gcagccgcct acccagaaag cagtacaacc ggcatcgcgg agccggcgaa
     3121 caacgtcgac agcgccatct cgcaaagtac ccatgccgtg ataggtcgct ggtttatcct
     3181 agcgttgtcc cgcacgctgc ccggtggact cgagccgcct gcgcaccagg actcgggcga
     3241 tttcgagctg ggtgtcgcct ggaaacggga cctgattcac tgtttggaca accggcacat
     3301 cgggcgtgag gcccgtacct tcgattgcgc gccctgcccg cgtcttgtac cgggaggtcg
     3361 taatcctgag cccccaccca gcttgaagga aatacaccgt ctgcacggtt cctttcccaa
     3421 acgttctcgc tcctacgaga ctgacaccgg cgtcctgaag cgcgcccgca acaatttctg
     3481 aagcgctcgc cgtgccgcga ttgaccagga ccacgacggg accggcaaac cggtgcgccg
     3541 ccggtcgcac gatgagcgtg atcgggccgc ggcgcccttc ttccactgcc accacgccct
     3601 gagagagaaa cgctgacgcc acattcacgg cctcttccac aaggccgccg ccgttctccc
     3661 gaagatcgag caccaagccc cgcgcatctt gcccgtccag acgaagaagc cagcgctcta
     3721 ggtcttcatg cgcccgctcc gtgaacccga ggagccggag gtaccccacc cgtggctcga
     3781 gcattcgtgc acgaaccggt atttctcgca cttgctcacg gatcaccgaa aagcgctgtg
     3841 gctggctcga agcccgctgg acgctgacaa ggacggtcga acccagccgc cctcgaagga
     3901 gagcgagcac cgtgtcggga gaacgaccct ccatcgacaa cccatcaatc tcttgaatct
     3961 gatcccccac acgaatcccg gcccgtactg cgggaccgcc ctcaacgacc tcgacgacgt
     4021 tgatcgcggt gccgactgta tctacctgca ggccgatcgt gccgtgttcg cctcgaaggt
     4081 ctgccatgta ccggcccagc tcagtgggcg tgaagacggc gctcacggga tccggcaccg
     4141 tgcggaccat cgcccgcaat accgcggcca ggatcaattc acctcgacct ggggccgatc
     4201 tgacggctgc ttcaacgtag acggcgacgg cgcgaaggtc ctcgtcttct gtccctgaga
     4261 gcaccggaac tgggggaact tcagtagcgc ccaagaggac ctgttgcacg ccggcgaccg
     4321 cggcgcgcaa gagcgaagga ccatcagatg cggcgagtgc gtcgtcgcgg aggcgtgtga
     4381 agacttcttg aacgagagcg gtaatcgcct cgtcgggcga ttgggcggca agctgtggcg
     4441 gcgccagcaa gagcgcgacc agcacgacca cccatgcgag caacgcaagc cgaacgggtt
     4501 tcatgttgtg gagaataaat ctgtagaacg gactaggctg aagcggcaag tgatgcggca
     4561 gtgcgaacgt tcatgatgat gccgccgccg agcacgacat ctctttcata gagggtcacg
     4621 gcctgaccgg gggccgctgc ccgctgcgct tcggcgaagt gcacaatcgc ctcgtcagag
     4681 gcgcggcccg gtcttacgac agcctcgacg tctcgggcgg cgtggcggac acgagcggtc
     4741 gcgagcaacg ccccgcgcag ttccggaaag gcgatccagt tcatcctcga cgccaccagt
     4801 tcgacacaga ggaggtcttc ttctcttccg acgatgaccg cgttccgttc tcgatctatc
     4861 ccgaccacat atagcggctc gccgtctgct attccgagcc cgcggcgctg cccgatcgtg
     4921 taccgcgcaa ttccctggtg ctgcccgatg acacggcccg acgtgtcgac cattgggccg
     4981 ggggcaagcg cttcggaacg gaatcgagcc acgacgtctg tgtatgtccc ccgaggcaca
     5041 aaacaaatct cctggctgtc cggtttgtcg gccacgggca aaccgaaacg tcgggccagg
     5101 ctgcgtgtct cctctttcgc ataaccgccg accggaaacc gcactcggcc aagctgcgcc
     5161 tgggtgagtc cgtacagcac gtacgactga tccttgtgcg gatccgctgc cctgagtacc
     5221 agccatcgac cactcttctc gtcgtgacgc aggcgggcgt agtgacccgt ggccaccccg
     5281 tccaggccaa gggcgctcac tttctcgagg agcaatgaaa acttgatgct ctggttgcag
     5341 gcgatgcacg gattgggcgt gcggccggac gcatactcgt cgacgaaata gccgatgacc
     5401 gcgcgctcga actcctcgcg catgttgagg acgtagtgcc ggataccaag ctggcgcgcg
     5461 gtcgcccgcg catcgtcgat cgccgagacg ccgcagcatg ccttgaaact gtcgtccggc
     5521 tcggggagcc agtctggcca caggttcatg gtgaggccca cgacctcgtg gccctcttcc
     5581 gcgagcagcg ccgcggccac tgagctgtcg actccgccgc tcatcgcgac ggcccactta
     5641 cccacactcc acctccgcac aaaaatacgg aaaactgtct tccatcataa caaggcttac
     5701 ccgcgggggt caaaagagac gctatccgat gggacgcttc aacaggtcaa tcgtcaacgc
     5761 cgccgtccac gagaattgat cggcgccggc gccccggccc gtgtacgcat cgaagtactc
     5821 gtgaaagccg ctgcttcgaa cgagttcaac cgtcgcttcc gccaggcggc gggcaggcga
     5881 acgcatcccc aggtcgtaca ggccgcgggc gagcatccag ttggcgttta cccacacggg
     5941 tccgcggtgg tagcgatctg actcgaagcc cgactgatcc gggagaaccg atggcagctg
     6001 acggcagttc cggcatcgat tcgcataggc gtcgtagatc ttctcagcct gcgctctctc
     6061 gacgagtccg gcgtagatcg ctgccaggcc ggcccacgta tgcaccggga tcttcgcctg
     6121 cgacgtaaga tcgaagtcga gaaacatcac gccctcggcg tcccacaacg cggcgtgata
     6181 ggcccgcccg aacgcatcca acgacgactt cgaaactacg ggcggccggt caattgcgag
     6241 ggcaattcga ttgaggtcct tcgccgcccg aaaccacacg gcgttgaaca tcgcgtcgta
     6301 cacagcgaac ggcgtcccgc caaggtaatt gcgcatctcg tagtcgtgcg ttgcgatcag
     6361 ttccaccagg aacatgtaaa gatcgtagtc gcgggacgtc gggcgctcgg cggccgccac
     6421 tttcgtggtg tccacccgcc ggtacggcct gctcggtctg accccttttg cgacggcggc
     6481 atcccatcga ggactattgt ccagtccgct ttcccacggg tgcacgagga cgatcaaagg
     6541 tgagttgccc gttgtacgat ggcgtgagaa gtagagcagg gactcagaaa gcgcatcata
     6601 gatgcgg
//
LOCUS       DBNGSB010000023         5159 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_1703225, whole genome shotgun sequence.
ACCESSION   DBNGSB010000023 DBNGSB010000000
VERSION     DBNGSB010000023.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5159)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_1703225"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..1159
                     /gene="galT"
                     /locus_tag="AC159B_00625"
     CDS             119..1159
                     /gene="galT"
                     /locus_tag="AC159B_00625"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HIR3376289.1"
                     /translation="MPELRKDPMSRRWVIIATERAARPTDFRQEDPEPSDLDKCPFCE
                     GREEKTPPEIFAFRAPGTAPNSPGWRVRVVSNKFPALRIEGQTQRTDVGIFTRMDGVG
                     AHEVIVETTNHHTHLGLLPPEHIADVVRAYLQRYRDLRGDTRFEYVLLFRNHRRRAGA
                     SLSHPHSQLIALPIVPKQAAEEVEAAELFFGREGTCIYCTMLKQEGEAKERLIFENER
                     FTVLAPYASRHPFETWILPKHHQADFGDLEPESEFSFAQALGETLRRLDSCLHNPPYN
                     FIIHTLPYKRETGHAYHWHVEIIPSLTHVAGFEWGSGFYINPVVPEEAARFLREVPSE
                     PVMAKPDSQAAG"
     gene            1203..1922
                     /locus_tag="AC159B_00630"
     CDS             1203..1922
                     /locus_tag="AC159B_00630"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018568498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR3376290.1"
                     /translation="MQVALFVTCLIDQLFPEVAEAAVSVLRRCGIEPALVQEQTCCGQ
                     IAFNDGFWPQAHILARRHLDLFESADAVVTPSGSCAAMVREFYPVLLKDDQALTARAH
                     HAGERTYELTEFLIDVLHRDDVGARFPHRVTYHASCHGLRGLGVRDQPFQLLRQVRDL
                     ELKSLDGLEECCGFGGMFAVKFSGLSGSMLDAKIRAIEASGAEVVTATDASCLMHIDG
                     GLRRRRSKVRALHLAEILASQ"
     gene            1919..3298
                     /locus_tag="AC159B_00635"
     CDS             1919..3298
                     /locus_tag="AC159B_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HIR3376291.1"
                     /translation="MKRIDFAKNSEKALRDPHLQTALRTAMVRFRVQRSSAVEEVGDW
                     QALRQYAHDVKAHTLANLDRYLDQLERQVTGVGGVVHWARDGHEARTLISGLVKASGR
                     RVVKSKSMTTEEIELNDALEHEGCEVTETDLGEYLVQLAGERPAHILAPVVHKSKEAI
                     AALLSEKLGVPRFDDARQLTMVAREALRRKFLEADVGISGVNFAVAETGSIVVVENEG
                     NARLTTTLPRTHIAVMGIEKVIPRMADLGVFLTLLPRSATGQRMSSYVSFLTGPRRAG
                     EVDGPEQLHLVLLDNGRTAIHADASMRESLACIRCGACLNVCPVFERTAGHAYGSQYS
                     GPIGAVLTPLFEGLEVAGDLPFASSLCGACGEVCPVKIELPRLLLELRSRVVRQRGAP
                     VAERLFVWGWVNAMTSARRLAIAGKVARAVHRFASAVRLPLPYPFSRWLSSRELPRPP
                     AKAFRERTQ"
     gene            3295..>5159
                     /locus_tag="AC159B_00640"
     CDS             3295..>5159
                     /locus_tag="AC159B_00640"
                     /inference="COORDINATES: protein motif:HMM:NF014910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HIR3376292.1"
                     /translation="MTTARDAILTRLQPAADPTIPLPTPSRLNAPPSDLVSLFIERAT
                     AVGTEIETTRSETLAPAIIDELTKTGASRVAMWEDRLLAQVRAALRSTPIEVLDPWDH
                     ARDKVPAADAGITIADFAIALTGTLVLDCSPAHPRSTSLLPPVHLAVVLGEHIVASLG
                     DMLARTGPLPSALTFITGPSRTADIELTPVRGAHGPTRVKVFLLLYWGSFYNGGTVMG
                     ITASLLSELREVLGSDYVLVEPEDVIVYERDGSIMQVMPEVVVLPADTAQVAGVVRAA
                     KRANVPIVPRGSGTGLAGGAVPAEGGVVVSLSRMNRILEVDPQNRTALLEAGVINLDV
                     TKAAAAHGLFYAPDPSSQAACSIGGNVANNSGGPHTLAYGVTTNHVLGVEVVLDDGQV
                     VWLGGRVPDTPGYDLCGVFVGSEGTVGIVTKALVRLMPTRETVQTLLAAFDRMDAATQ
                     SVVEITGRGIIPAALEMMDKVTIEAVEMGASLGYPKGAEAVLLVEVEGVREHCARAVA
                     LVREICEQKGARQVKVAQDTAERQRLWKGRKEAFGSMGRLAPHYYVQDGVVPRSRLPE
                     IMREVAAISTRFGLRIANVFHAGDGNLHPNILFDMRKAGELDRVIEASDAILRACV"
BASE COUNT          961 a         1586 c         1696 g          916 t
ORIGIN      
        1 gcgcacggga atgcgggcag atttctcctg gggccgatcg gcgcgaaagt acctggagct
       61 ctacgagcgt gctcaagcca gggcagccgc tctgccgcag ggctaggagg gaatctgcgt
      121 gccagaactg cgcaaagatc cgatgagccg gcgctgggtg attatcgcta ccgagcgggc
      181 agcccggccc acggattttc gtcaggagga tccggaaccg agcgatctgg acaaatgccc
      241 gttctgcgag gggcgtgagg aaaagacccc gcctgagatc tttgccttcc gcgctcccgg
      301 caccgcaccc aatagcccgg ggtggcgagt ccgcgtcgtc tccaacaagt ttcctgcgct
      361 ccgcatcgag gggcagacgc aacggacgga cgtcggtatt ttcacgcgca tggatggcgt
      421 gggcgcgcac gaagtgatcg tggaaacgac caaccatcac acccacttgg gtttgcttcc
      481 acccgagcac atcgctgatg tcgtgcgggc gtatctccag cgataccgcg accttcgcgg
      541 tgatacgcga ttcgagtacg tgctgctgtt ccgaaatcac cgccggcgcg ccggcgcgtc
      601 gctctcacac ccgcactcgc agctgatcgc gctcccgatc gttcccaagc aggccgccga
      661 ggaggtggaa gcggcagagc tcttcttcgg ccgggagggc acgtgcatct actgcaccat
      721 gctaaagcag gagggcgagg ccaaggagcg tctcatcttc gagaacgagc gatttacggt
      781 cctggcgccc tatgcctccc ggcatccgtt cgagacctgg atcctgccca agcaccatca
      841 ggccgacttc ggtgatctcg aacccgagtc agagttctcg ttcgcgcagg cactgggaga
      901 aacgctgagg cggctcgaca gctgtctgca caacccgccg tacaatttca tcattcacac
      961 cctgccgtac aaacgggaga cagggcatgc gtaccactgg catgtcgaga tcatcccgag
     1021 cctcactcac gtggccggct ttgagtgggg atccggattc tatatcaacc ccgtggttcc
     1081 ggaagaagcg gcccgcttcc tgagagaggt gccgtccgaa ccggtcatgg cgaaaccaga
     1141 cagccaggca gcggggtagc gaagcggccg gtagccaggg gagccgggta gccagggagc
     1201 acatgcaagt cgcattgttt gtgacctgtc tgatcgatca actgtttcct gaagtcgccg
     1261 aagctgccgt cagtgtcctc cggcgttgcg ggatcgagcc ggcgcttgtg caggaacaga
     1321 cttgctgcgg acagatcgca ttcaatgacg gcttctggcc gcaggcgcac attctggcac
     1381 gcaggcactt ggatcttttt gagagcgctg atgccgtcgt gacacccagc ggttcatgcg
     1441 ccgcgatggt gcgtgaattc taccccgtgc tcctcaagga tgatcaggca ctgaccgcgc
     1501 gcgcacatca cgcgggagag cgcacgtacg agctcaccga attcctcatc gatgtgctcc
     1561 accgagatga tgtcggggcg cggttccctc accgcgtcac gtaccatgcg tcctgccacg
     1621 gactacgggg actcggtgtt cgggatcagc cgttccagct gttgaggcag gttcgagatc
     1681 tggaactgaa atcgctcgat ggactggaag agtgctgtgg gtttggcggc atgttcgcgg
     1741 tcaagttctc gggactgtcc ggctcgatgc tcgacgcaaa gatccgtgcg atcgaagcct
     1801 ccggcgccga agtcgtgacc gccacggatg cgagttgttt gatgcacatc gacggcggac
     1861 tcagacggag aaggagtaag gtgcgagcgc tccatttggc ggagattctg gcgtcccagt
     1921 gaagcgaatt gacttcgcaa aaaactcaga gaaggcgttg cgggacccac acctgcagac
     1981 cgcactgcgg accgcgatgg tgcggttccg ggttcagcgg agcagcgcgg tcgaagaggt
     2041 cggggactgg caggcgctgc gacaatacgc ccacgacgtg aaggctcaca cgctggccaa
     2101 cctcgacaga tatctggatc agctggagcg gcaggtgacg ggcgtgggcg gggtggtcca
     2161 ttgggcccgc gacggacacg aagcccgcac tctcatttct gggctcgtca aggcaagcgg
     2221 aaggcgggtc gtcaagagca aatcgatgac gaccgaggag atcgagctca acgatgcgct
     2281 cgagcatgaa ggatgcgagg tgaccgagac ggatctcggc gagtacctgg tgcaactcgc
     2341 cggagagcgt cccgcgcaca tccttgcgcc ggtggtgcac aagtcgaagg aggccattgc
     2401 cgcgctgctg tccgagaagc tcggcgtgcc ccggtttgac gacgctcgtc aattgacaat
     2461 ggtggcgcga gaagcgctcc gccggaaatt cctggaagcg gacgtgggga tcagcggggt
     2521 gaactttgcc gtcgcggaga ccggctcgat tgttgtggtg gaaaacgaag gcaacgcgcg
     2581 cctcacgacg acgctgcctc ggacacacat cgccgtgatg ggtatcgaga aggtgatccc
     2641 ccggatggcg gacctcggtg tattcctcac actgctcccc cgcagcgcca ccggtcagcg
     2701 aatgagcagt tacgtgtcgt ttctcacggg accgcggcgc gccggcgagg tcgatggtcc
     2761 cgagcagctg cacctggtgc tgctcgacaa cggacgcacg gccatccacg cggacgccag
     2821 catgcgggag tcgctggcgt gtatccgctg cggagcgtgt ctcaacgtgt gcccggtatt
     2881 cgagcgcacg gcgggccacg catacggttc gcaatactcg ggaccaatcg gggcggtgct
     2941 gaccccgctg ttcgagggcc tcgaggtggc gggagatctg cccttcgcat catctctatg
     3001 cggagcctgc ggtgaagtgt gtcccgtaaa gatcgagctg cccagactcc tcctggagct
     3061 gcggtcccgt gtcgtccggc agcgcggtgc accggttgcg gaacgactct tcgtgtgggg
     3121 atgggtgaac gcaatgacga gcgccagacg cctcgccatc gccggaaaag tggccagggc
     3181 ggtccaccgt ttcgcgtcag ccgttcgcct tccgctgccg tatccattct cgaggtggtt
     3241 gtcgtctcga gaacttccca ggccccccgc caaggcattc agagagcgaa cacaatgacg
     3301 accgctcgag atgcgatcct gacccgcctg cagccggccg cggatccaac gatcccgttg
     3361 cccactccgt cgcggctcaa tgcgccgccg tctgatcttg tgtctttgtt cattgaacga
     3421 gccacggccg tggggacgga gatcgagacc accaggtctg agacactggc gcccgcgatc
     3481 atcgatgagt tgacgaagac cggggcgagc cgcgtcgcca tgtgggaaga ccggctgctc
     3541 gcgcaggtcc gggcggcgct tcgctcgacg ccgatcgaag tgctggaccc atgggatcac
     3601 gcacgcgaca aagtgcccgc agcggatgcg ggcatcacca tcgcggattt cgccatcgcg
     3661 ctcaccggta cgctggtcct ggattgctca ccagcgcatc cacgcagcac ctccctcctt
     3721 cctcctgtgc acctggctgt ggttctgggt gagcacatcg tcgcctcgct cggtgacatg
     3781 ctggcgcgaa cggggccttt gccttccgcg ctgacgttca tcacggggcc gagccgcact
     3841 gccgacatcg aactgacccc ggtgcgtggc gcacacgggc cgactcgggt gaaggtgttt
     3901 cttctactat actgggggtc attctataat gggggcacgg taatggggat cacggcatca
     3961 ctcctcagcg aactacgcga agtcctggga tcggattacg tcctcgttga gcccgaggac
     4021 gtcatcgtct acgagcgcga tggctcgatc atgcaggtga tgccggaggt cgtcgtcctg
     4081 ccggccgaca ccgcccaggt tgcgggggtc gtgcgcgccg cgaagagagc aaacgtcccg
     4141 atcgtacccc gtggatccgg aaccgggctg gccggcggcg ccgtgccggc cgagggcggc
     4201 gtggtcgtgt cactgtcgcg catgaaccgg atcctcgagg tcgaccccca gaaccgcact
     4261 gcgctgctgg aagccggcgt catcaacctc gacgtcacga aggccgcagc ggcccatggt
     4321 ctcttctatg cgcccgatcc gagcagccag gccgcctgtt cgatcggcgg caatgtggcc
     4381 aataactccg gcggtccgca cacattggcc tatggggtaa ccacgaacca cgtgctcgga
     4441 gtcgaagtcg tgctcgacga cggacaagtc gtctggctcg ggggcagagt gccggacacg
     4501 ccgggctatg atctgtgcgg cgtcttcgtt ggatcagaag ggacggttgg aatcgtcacc
     4561 aaggcgctcg tccgattgat gccgacgcgc gaaacggtcc agacgcttct tgccgcgttc
     4621 gaccgcatgg atgccgcgac gcaatcggtt gtggagatca cgggacgagg gatcattccg
     4681 gccgcgctcg agatgatgga caaggtcacg atcgaggccg tagaaatggg ggcgtcgctg
     4741 ggctatccca aaggcgccga ggccgtgctc ctcgtggagg tcgaaggcgt ccgggagcac
     4801 tgcgcccgcg cagtggcgct cgtccgggag atctgcgagc aaaaaggcgc ccggcaggtc
     4861 aaggtcgcgc aggatacggc agagcgccag cgtctatgga aaggacgcaa ggaagcgttt
     4921 ggatcaatgg gacgccttgc cccccactat tacgttcagg acggcgtcgt cccgcggagc
     4981 cgcctccccg agatcatgag agaagtcgca gcgatcagca cgcgattcgg gttgcgcatc
     5041 gccaacgtgt ttcacgccgg ggacggcaac ctccatccga atatcctgtt tgacatgcgc
     5101 aaagctggcg agctggaccg tgtgatcgag gccagcgacg ccatccttcg cgcgtgcgt
//
LOCUS       DBNGSB010000024        13366 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_170320, whole genome shotgun sequence.
ACCESSION   DBNGSB010000024 DBNGSB010000000
VERSION     DBNGSB010000024.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 13366)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13366)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13366
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_170320"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="AC159B_00645"
     CDS             <1..626
                     /locus_tag="AC159B_00645"
                     /inference="COORDINATES: protein motif:HMM:NF018984.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HIR3376293.1"
                     /translation="FSPKYPFLGRHIALDFANTVEWHASNLPQDRIGSYSDLMAWALQ
                     SGTLSEGQAAYFVDAQTVRPDEAATALQVARDLRESIYRLMPATSTRGSPAPADIDTL
                     NRVLREGFLRLKVSAVQNAFGLQWAEDDVLMRPLWPVAWAAAQLLTSPDLARVKQCAS
                     TDGCGWLFLDRSKNLSRRWCDMRACGNTAKARRYRKRQESGADGAAI"
     gene            complement(619..1467)
                     /locus_tag="AC159B_00650"
     CDS             complement(619..1467)
                     /locus_tag="AC159B_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR3376294.1"
                     /translation="MPTPNRRRALGKGLGALIPGGGAAAGQPRTDIPLTRLVANPLQP
                     RQAMGQEELADLTSSVKQHGVLQPIVVRPIRDSFEVVAGERRWRAAEAAGLQTIPAVV
                     MALTDQEALELALVENLQREDLNSMERARAYRRLNDDFGLTQEQIADRVGKSQPSVAN
                     ALRLLKLPPQIQAALEIGRITEGHARAILSVSTDTGMLRLWERVERRGLSVREAERLA
                     RGSISREIQPRVLPKDPQLRSTEQDLSRRYGTKVVIHGTRRRGTLLFEYYSEEDLQRM
                     LDLLLK"
     gene            complement(1493..2275)
                     /locus_tag="AC159B_00655"
     CDS             complement(1493..2275)
                     /locus_tag="AC159B_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR3376295.1"
                     /translation="MNRVIAVANQKGGVGKTTTAVNLSAALALTGQRILVVDLDPQGH
                     ATSGLGVPRNGAGPTVYDTLIDDAPLLSVIVPTQILGLDLVPGSIHLAGAEVELVSLP
                     SRETRLRDAIGSVRDRFDAVLIDCPPSLGLLTLNALVAADEVIIPIQCEYYALEGLTR
                     LVDTVSLIRRDLNPALRIGGVLLTMYDPRTNLAEQVAVEIRRHFAGEVFQTAIPRSVR
                     LAEAPSFGQPVVTYDPSSKGAAAYLALAKEVVERVGQPAAVS"
     gene            complement(2446..3189)
                     /gene="rsmG"
                     /locus_tag="AC159B_00660"
     CDS             complement(2446..3189)
                     /gene="rsmG"
                     /locus_tag="AC159B_00660"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HIR3376296.1"
                     /translation="MNLNEAGLKSHLRGGAATLGLELTNAQSDAFLHYLTLLQRWNRR
                     ARLTALTESVSVIQLHFLDSLAVLRAPVPQHASVIDVGSGAGFPGIPLAIARPDLHVS
                     LLEPAARKAAFLELASSELKIRTTVRHSRAEIAGHDPEWRGQFDVATGRAIAKSAILA
                     ELLLPFVKIGGSAILLKGPAAQADVVELQQSSSVLGGRLLDPMRVTLPRGERRILIVI
                     SKIAATPERFPRQTVLHTRSRVIPKETPS"
     gene            complement(3176..3814)
                     /gene="jag"
                     /locus_tag="AC159B_00665"
     CDS             complement(3176..3814)
                     /gene="jag"
                     /locus_tag="AC159B_00665"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HIR3376297.1"
                     /translation="MKDLETSGRTVDEAVERALGELGLTRDDVDIEVVDSGARGLLGL
                     GGRDARVLVRPRVMPSIAAHQLAEDLLEAMGFRGTIRVREQEGAVGVEVSGQNLAALI
                     GRHGTTLEAMGVLLEAMVAKRTGHRPRILVDVAGYRERRRTALEGIGKRAADRAIREE
                     REIALEPMASYERRIIHTLLAEHPQVVTFSRGEGSERHIVVAPRNQAMNQPE"
     gene            complement(3922..4677)
                     /locus_tag="AC159B_00670"
     CDS             complement(3922..4677)
                     /locus_tag="AC159B_00670"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HIR3376298.1"
                     /translation="MGFIDAIANVLANVLSVFTVWTGSAILAITLLTLGVKLILHPLT
                     RKQLKSMKAMHALTPHIAALREKYRGNPQQMNTEMMSLYRAHNVNPLGGCLPLLLQMP
                     ILWALFTVFRRPGVFSGETFFGIPLERVPVSDGNFFSSNFWVGLWNVIFQTPVVAVLI
                     VLVGLTTYLQQRQSVTDPQQARMFVFMPIMFSVFAVAFPVGMSIYWIIYSVLGTLEYL
                     LVAGRPSAPSAVPARTTEKPEIFSQRPKNSRKK"
     gene            complement(4692..4904)
                     /gene="yidD"
                     /locus_tag="AC159B_00675"
     CDS             complement(4692..4904)
                     /gene="yidD"
                     /locus_tag="AC159B_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010873664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HIR3376299.1"
                     /translation="MLTKLLLLMVRAYQRFISPFTPPTCRFSPSCSEYALQAVSRHGA
                     WKGSRLVIARLLRCHPLHPGGYDPVP"
     gene            complement(4924..5250)
                     /gene="rnpA"
                     /locus_tag="AC159B_00680"
     CDS             complement(4924..5250)
                     /gene="rnpA"
                     /locus_tag="AC159B_00680"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HIR3376300.1"
                     /translation="MVNRLRSSEQFRLAYREGRRASNDFFTVHARRNGLSQSRVGIAV
                     SGRIGKAVDRNRVRRRIREVFRSLPDIPAGVDVVVVAKGAVKKAAVPDLIEGARDLVR
                     RTAGDS"
     gene            complement(5253..5387)
                     /gene="rpmH"
                     /locus_tag="AC159B_00685"
     CDS             complement(5253..5387)
                     /gene="rpmH"
                     /locus_tag="AC159B_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HIR3376301.1"
                     /translation="MKRTYQPKARKRKRSHGFRSRMRTPGGRTVLKRRKAKGRHRLTV
                     "
     gene            5867..6655
                     /locus_tag="AC159B_00690"
     CDS             5867..6655
                     /locus_tag="AC159B_00690"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3376302.1"
                     /translation="MSDFVRLLHEGRPRTEATSSTETEESTEVRLAVYDSPMAAPRVV
                     SLRGQEFHEFVGELAARTYHFSHERGGRIPYVVIREVIENLIHAYFEGAVISILGDGN
                     TIRISDQGPGIAEREKALLPGFTTATPEMRRFIKGVGSGLPVAHEQLEFLGGAIAIDD
                     NLGRGTVITLSVGATPPRSAPALRATAIPPTLSARQKKVLLLIAELGSAGPSAIAKEL
                     GISQSTAYRELQVLVDLRLVNKKRQGKRTLTEEGVAVLGGVFQP"
     gene            6713..8047
                     /gene="dnaA"
                     /locus_tag="AC159B_00695"
     CDS             6713..8047
                     /gene="dnaA"
                     /locus_tag="AC159B_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019120830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HIR3376303.1"
                     /translation="MAVDTTSVTGVWQAALRRIEGRLSKPSYESFVKAMAPMSLEGDV
                     FTFSLPSRLAKEWVETRYSGLIRAALQEVLARPVGIQLVIAEEVPPAPALRPPQRIPD
                     GLQLSPKYTFDTFVIGSGNRFAHAAAMAVAEAPARAYNPLFIYGGVGLGKTHLLQAIG
                     HHVIHRKQLHRVAYISSEKFTNELINSIRDDRTLEFRTKYRNVDVLLIDDIQFLAGKE
                     RTQEEFFHTFNTLHEASRQIIISSDRPPKEIPTLEDRLRSRFEWGLIADIQPPDVETR
                     IAILRKKAEVDGIGVPDEVAEFIAQRIQSNIRELEGALVRVVAYANLTRSTITVDLAS
                     EILKELLPVSPARPITIPSIQKAVAAHFGIRVEEMRAKRRTKGVAFPRQVAMYMAREL
                     TDASLPRIGEEFGGRDHTTVMHACDRVKEALSQDSHLVATLKSVVDTLKTGE"
     gene            8204..9313
                     /gene="dnaN"
                     /locus_tag="AC159B_00700"
     CDS             8204..9313
                     /gene="dnaN"
                     /locus_tag="AC159B_00700"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HIR3376304.1"
                     /translation="MWVTCSQSALLKGVHLVNRAISTRTTMPILSYVLLETTKSGLKL
                     TATDLELAIQVEIDGEVKQGGQLTLPARILTEIVGNLPESTVEIKADEGVTQTQITCE
                     NSNFEILGLPAGDFPTMPKPAEDALGAIDAESMRTMIRQTIFAVSGDETRPFLTGVYV
                     VMDGTDVRFVATDGGRLALRKTTLPSPVNQKTGVIVPAKTMQELARALGGMEGDVQLA
                     AVDNQLMFAVPGLRVYSRLITGQFPNYQQVIPQEFKQRIRVDTDRLLRAVRRASITAR
                     DSANVVRLAAKGKKLTITSNTPEVGKAREDVEVTAEGDTVEAAFNARYLIDCLNNIDA
                     ADLYFELTGPLSPGAIRPAEHEDYVYVLAPVRVYG"
     gene            9377..9598
                     /locus_tag="AC159B_00705"
     CDS             9377..9598
                     /locus_tag="AC159B_00705"
                     /inference="COORDINATES: protein motif:HMM:NF024672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HIR3376305.1"
                     /translation="MAAERNVAIRTESIELAALLKWAQAVNSGGEAKQLIQDGHVRVN
                     GEVEYRRARTVTIGDRVEIDDIVLIVTKR"
     gene            9598..10725
                     /gene="recF"
                     /locus_tag="AC159B_00710"
     CDS             9598..10725
                     /gene="recF"
                     /locus_tag="AC159B_00710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.2"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HIR3376306.1"
                     /translation="MHLTHLRLINFRNHARTEIEPGPRLNCFVGQNAQGKSSILEAVE
                     IAATGRSHRAVRDTDLLHFGEAWARVRAAVRRGDRDVEVDIVWRTETSTAGAKEFRVN
                     GVPVRRGDLFGHVLIVASTPHDQEVVLGAPAHRRRLLDLLLSQISPAYFFLLLRYARA
                     LEQRNRLLRDLAGPAREREAALDPWDEQLSTMGAAITARRLVIVTRLSQAAREAYAFL
                     ASDQEAVSIAYAPSLSASDETAMITMARGAFARRRREEVIRGTTLTGPHRDDLRMEID
                     GRDLRTFGSRGQHQAAMLSLRLAERRILLEESGEEPVLLLDDAAQLLDEERQVRLMQL
                     VGRAQALLTLTTTAALRHVPPDTTVFKVAGGRVEPQLAYPT"
     gene            10709..11002
                     /locus_tag="AC159B_00715"
     CDS             10709..11002
                     /locus_tag="AC159B_00715"
                     /inference="COORDINATES: protein motif:HMM:NF017103.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HIR3376307.1"
                     /translation="MLTPLRSILKTAAKSLGLERAANAAFVQELWPEVAGPEAAAHTR
                     VQGLRGRTLLIDAEPGPWAQDLTAQRARYVREFNRRLGEDVVEEIRFRQRPLT"
     gene            11010..11291
                     /gene="remB"
                     /locus_tag="AC159B_00720"
     CDS             11010..11291
                     /gene="remB"
                     /locus_tag="AC159B_00720"
                     /inference="COORDINATES: protein motif:HMM:NF046065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular matrix regulator RemB"
                     /protein_id="HIR3376308.1"
                     /translation="MYVHLGGDVVVPAKDVVAILDARLLGQSEVNRELVERAVTAKKL
                     HGPGIGPGCKALVVAVDAVYTSGISAPTLARRMVHFRQSALSWEAETQV"
     gene            11505..>13366
                     /gene="gyrB"
                     /locus_tag="AC159B_00725"
     CDS             11505..>13366
                     /gene="gyrB"
                     /locus_tag="AC159B_00725"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011876402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HIR3376309.1"
                     /translation="MATNQTTTPYTAEQIQVLEGLEGVRKRPAMYIGSTGPDGLHHLL
                     YEVVDNSVDEALAGRCDEIAVILHKDDSASVIDNGAGIPVDPVPKIGKPAVEVVLGTL
                     HAGGKFGGGGYKISGGLHGVGVSVVNALSEWLEVEVWRDGKTYKQRFARGKKTTPLEQ
                     VGNTRKKGTRVTFRADPEVMTAMEFNAEVIAGRLEDIAYLNKGLTIRLHDERSDKEQV
                     FKHAGGIAELVQALNRTKTKVSAVMQLTKERDGVEVEVALQYSESYLEHTLTYVNTIP
                     TTEGGTHVAGFRSALTRAINDYARRSGLLKNGDVTLMGDDVREGLTAVLSLKLPEPQF
                     EGQTKTKLGNTEVKGLVESVVGDWLQEWLETHPGDAKRVVAKALTAARAREAAKQARD
                     LVRRKTALEVSPLPGKLADCVETDPERAEIFIVEGESAGGSAKQGRDRQFQAILPIQG
                     KILNVEKAREDKMVSHEEIRAIITAVGTGFGEKFNFDERRYDRVIIMCDADVDGSHIR
                     TLLLTFFYRYMRQLVEHGKVYIAQPPLYLVRSGKDRRYAYSDEARQVAVKEFEAKGGK
                     PEVQRYKGLAEMNPEQLWETTMNPETRTLLRVEIEDAAAADDIFTKLMGEEVEPR"
BASE COUNT         2704 a         4065 c         4051 g         2546 t
ORIGIN      
        1 acttctcgcc aaagtatccc ttcctaggtc gccacattgc cttagacttt gccaacacgg
       61 tggagtggca tgcctcgaac ctccctcagg acaggatagg gtcttactca gatctcatgg
      121 catgggcgct gcaatccggc actttgtccg aagggcaggc agcctacttt gtcgatgcgc
      181 agacggtgcg tccggacgag gcagcgaccg cgcttcaggt tgctcgagat cttcgcgaat
      241 caatctaccg gctgatgccg gccacgtcga ccagggggtc accggcgccg gcagacattg
      301 acactctgaa ccgcgtgctt cgcgagggat ttctgaggtt gaaggtcagc gccgttcaaa
      361 acgccttcgg gcttcagtgg gctgaggatg acgtcctgat gcgtccgctc tggcccgtcg
      421 cctgggctgc ggcccagctt cttacgtcgc cagacctggc cagggtgaag caatgtgcct
      481 cgaccgacgg atgcggctgg ctcttcctgg atcggtcgaa gaacctctct cgccgttggt
      541 gcgacatgcg ggcatgcggc aataccgcaa aggcgaggcg gtatcggaag cggcaggagt
      601 caggagcgga cggcgctgct atttgaggag caggtccagc atcctttgca ggtcttcctc
      661 ggaatagtac tcgaagagca gggtgcctct gcgccgcgtt ccatggatga caaccttcgt
      721 cccgtaacgt ctggaaaggt cctgttcggt gcttctcagc tgaggatcct tcgggagcac
      781 gcgcggctgt atttcacgtg aaatacttcc cctggccagt cgttcagctt cgcggacgga
      841 gagcccgcgc cgctcgactc gctcccaaag ccgcagcatc ccagtgtcag tcgatacaga
      901 gagaatggcc cgcgcatgcc cctctgtgat tcttcctatt tccagcgcag cctggatctg
      961 cggcggtagt ttcaagagcc gtagggcatt cgcgaccgaa ggctggctct tcccgacccg
     1021 atcagcgatt tgctcctggg tcagaccgaa atcgtcgttc agccttctgt acgcccgcgc
     1081 tcgctccatc gaattcaggt cttcccgctg aaggttctct actagcgcaa gctccagcgc
     1141 ttcctgatcc gtcaacgcca tgaccacggc cggaattgtc tgaagaccgg cagcctcagc
     1201 cgcccgccag cggcgctctc ccgctacaac ctcgaagcta tctcgaattg gtcgaacgac
     1261 aatgggctga aggacgccgt gctgtttgac cgagctcgtg agatctgcta gctcctcctg
     1321 acccattgcc tgtctgggtt gaagggggtt agccaccagc ctggtcaaag gtatatcggt
     1381 ccttggctga ccagcggcgg ctccgcctcc aggaatgagc gcgccaagtc ctttcccaag
     1441 agcccgtctg cggttcggcg tgggcatcgg agtgcgcgac agatctgggg tctcagctga
     1501 cggcagccgg ctgtccgaca cgctccacca cctcctttgc gagggccaga taggctgccg
     1561 ccccctttga tgagggatcg tatgtcacga ccggctgacc aaagctcgga gcttccgcca
     1621 gtctgacact gcgaggaatc gcagtttgaa acacttcgcc cgcgaaatgc cgtctgatct
     1681 cgacagcgac ctgctcggca aggttggtgc gcggatcgta catggtgagg agcacgccgc
     1741 caattctgag ggccgggttc agatctcgac ggatcagcga caccgtatcg acgaggcggg
     1801 tcagtccctc gagcgcgtag tactcgcatt gaatggggat gataacctca tccgcggcga
     1861 ccagcgcatt gagtgtgagc agaccgagtg aaggcggaca gtcgatgagc acggcatcaa
     1921 agcgatcgcg cacggatccg atcgcgtctc ggagccgggt ctcgcgcgaa ggaagcgaca
     1981 cgagttcgac ttcggcgccg gcaaggtgaa tgctgccggg taccagatca agtccgagta
     2041 tctgcgttgg aacgatgacc gagaggaggg gagcatcgtc gatgagcgtg tcatacaccg
     2101 tcgggccggc gccgtttcgg ggaacaccca gtccgctcgt ggcatgtccc tgcggatcca
     2161 ggtccacgac cagaattcgc tgaccggtca gggccaaggc cgctgagagg ttgaccgccg
     2221 tggtcgtctt cccaacgccg cccttctgat tcgctaccgc gatgacacga ttcatccaac
     2281 cgcaacgtgc aaaattcaac ggcctcgccc tcggctcggc cttccgactc aatcaacgac
     2341 caagctctac tagcctcgcc cgccggctcg gcttcctcaa cagccggcgg cccactccgt
     2401 gggcgggctt tgtcgtcgac ttgcgcccca aactgccatt cggtactacg aaggggtctc
     2461 ctttggaatc acccgactgc gagtatgcag gacggtctgc cgcgggaacc gctctggcgt
     2521 tgccgcaatc tttgagatta cgatcaaaat tcgccgctct ccccgcggca gcgtgacacg
     2581 cataggatcc agaagcctcc caccgagaac ggacgaagat tgttgcagtt ctactacgtc
     2641 cgcttgcgcc gccggccctt tgagcaaaat cgcgctgcct ccaattttca cgaacgggag
     2701 cagcagctct gccagaatcg cggactttgc gatggctctg ccggtcgcga catcgaactg
     2761 tccccgccat tccgggtcgt gaccggcaat ctctgcgcga gagtgccgca ccgtcgtgcg
     2821 gatctttaac tcggaactgg cgagttccag gaacgcggct ttccttgctg caggttcgag
     2881 cagcgagacg tgcaggtcag ggcgagcgat cgcgaggggg ataccgggga agcctgctcc
     2941 gctgccgaca tcgatgacgc tcgcgtgctg cggaactggc gcgcggagga cggcgagtga
     3001 gtcgaggaag tggagctgga tcactgacac actttccgtg agcgccgtca ggcgagcgcg
     3061 gcggttccag cgctgcaata gcgttaggta atgtaggaag gcgtcgctct gcgcgttggt
     3121 cagctcaagc ccgagcgtcg cggctccacc gcgcaggtgg ctcttcaggc cagcttcatt
     3181 caggttgatt catcgcctga tttcgcggcg cgaccacgat gtgccgctcg ctaccttctc
     3241 cccggctgaa cgtgacgacc tgcggatgtt cggcgagcag cgtatgaatg atgcgtcgtt
     3301 catatgaagc catcggctcg agcgcgatct cacgttcttc gcggatggcg cggtctgccg
     3361 cgcgcttccc tattccttca agcgccgtcc ggcggcgttc ccgataacca gcaacatcga
     3421 ccaggatccg cgggcgatgt ccggtgcgtt tggcaaccat cgcctccagc aggactccca
     3481 tcgcttcgag cgtcgttccg tggcggccga tcagagccgc aaggttctgg cctgacacct
     3541 ccacgcccac ggcgccttcc tgctcccgca cacgaatcgt gccacgaaat cccatcgcct
     3601 cgaggagatc ctcggcaagc tggtgcgcag cgatcgacgg catgacgcga ggccgcacga
     3661 gcacgcgcgc gtcacgccca cccaaaccga gcaggcctcg tgcaccggag tcgacgacct
     3721 cgatgtcgac gtcgtcccgg gtgaggccga gttctcctaa cgcgcgctcg acggcttcat
     3781 caaccgtccg accgctggtc tctaaatctt tcatttcttt ttacccggtc tactgctgcg
     3841 gcgagggggt gttgcgaagc caaaggggtc cccgtattag ttgcagtcgg ggaaaggctt
     3901 cgcgactccg tctcgccgcc ttcatttctt tctcgaattt ttcgggcgct gcgaaaagat
     3961 ctcgggcttc tcggtcgtac gcgccggcac agcgctgggc gcggatggtc gcccggcgac
     4021 gaggaggtat tccaacgtgc ccaggacgct atagataatc cagtagatcg acatgccgac
     4081 gggaaacgca accgcgaaaa ccgagaacat aataggcatg aagacgaaca tccttgcctg
     4141 ctgcgggtcc gtgacggact gccgctgctg cagatacgtc gtcaggccca cgaggacgat
     4201 cagcaccgcc acaaccggcg tctggaagat cacgttccac agaccaaccc agaagttgct
     4261 ggaaaagaaa ttcccgtcag acacggggac ccgctcgagc gggatcccga aaaacgtctc
     4321 tccactgaac acgcccggcc gcctgaagac ggtgaacaat gcccacaaga tcggcatctg
     4381 cagcagcagc gggagacatc cccccaacgg gttcacgttg tgcgcccgat agagactcat
     4441 catttctgtg ttcatctgct gcgggttgcc acggtacttc tctcgcagcg cagcgatgtg
     4501 cggggtcagc gcgtgcatcg ccttcatcga cttcaactgc ttgcgagtca gcggatggag
     4561 gatgagcttg acgcccagcg tgagcagcgt aatggcaagg atggcgctgc cggtccatac
     4621 ggtgaacacc gacagcacgt tcgccagcac attggctatt gcatcaatga atcccacggc
     4681 ctcaactcaa ctcaaggaac cgggtcatat ccgcccggat gaagtgggtg acatcgcaac
     4741 agccgcgcga tcacgaggcg cgagcctttc caggcgccat gccggctgac cgcctgcagc
     4801 gcgtactcgg agcaggatgg agagaaccgg cacgtcggcg gcgtgaatgg tgaaatgaat
     4861 cgttgatacg cgcgaaccat caggagcagt aacttcgtca acacgtacga ctcaccctta
     4921 ttatcagctg tcgccagcgg tacgccgcac caaatcccgg gcgccctcga tcaaatcggg
     4981 aacagccgcc tttttcaccg cgccctttgc gacgacgaca acatcgactc cggcgggaat
     5041 atctggcagc gaccggaata cttcccggat cctgcgacgg acacggttcc gatccaccgc
     5101 cttcccgatg cgcccggaaa ctgcgatccc cactctcgac tgcgacagcc cattccggcg
     5161 agcgtggacg gtaaagaaat cgttcgatgc acggcgccct tcccgatatg ccagccgaaa
     5221 ctgctcactc gatctgagac gattgaccac tcttacacgg tgagtcggtg gcgccccttc
     5281 gcttttcgcc ttttcagaac ggtgcgcccc ccaggagttc gcattctgct ccgaaacccg
     5341 tgagagcgct tccttttgcg tgctttcggc tggtaggtac gcttcatcgg caggccctcg
     5401 gcacatcact atagcacacg ccgcaatcga tcccgaacgt ctatgccttt catctcctgt
     5461 ggacaacctg cgcataacaa gggggtttct ccacagattt tcgaccacag atcctgtcat
     5521 gatccccccg atcactccct cccgtcgccc cgggtcttgt ggacagggcc gggtccctcc
     5581 ctaaccgccg catgttatcc acagagtttt tcacagctgt ggataagtgc gcaggcccgc
     5641 gtctggtggg ctcaacgcac tcacgttcgg caggagcgct ctcttgcgcc gacgaatttc
     5701 ctgccactcc cggcgtcaag ttgtccacat tcgtggtcac ctgctgcccg tccatgggtg
     5761 gttatgaaat gcactctgtc gattgtttgt cggtaggagt tcccacagga acggataact
     5821 tccacgagca tcgcgtgcgg ggcgaactga tctctggcgt tatgtgatgt ctgacttcgt
     5881 ccgtctcctc cacgagggcc ggccccggac agaggctacg agctcgacgg agacggagga
     5941 atcaacggaa gttcgcctgg cagtctatga ctcgccgatg gctgcgccgc gggtcgtctc
     6001 gcttcggggg caggagtttc acgagtttgt cggagagttg gcggcgcgca cgtatcactt
     6061 ctcacacgaa cggggcggcc ggattccgta cgtggtgatc cgcgaagtga tcgagaacct
     6121 tattcacgcc tatttcgagg gtgctgtgat cagtatcctc ggcgacggca acaccattcg
     6181 catctcagat caaggcccag ggattgcgga gcgggagaaa gcgctgctgc cgggcttcac
     6241 caccgctacc cctgaaatgc gccgattcat caagggggtt gggagtgggc tcccggttgc
     6301 gcacgagcag ttggagtttc tcggtggcgc catcgccatc gacgacaacc tcggacgagg
     6361 aacggtcatc acgctgtcgg tcggcgcgac gccgcctcgg agcgcgcccg cacttcgagc
     6421 gacggccatc ccaccaacac tgtctgcccg gcagaagaag gtgctgctcc tcatcgccga
     6481 gttgggctca gccggtcctt cggccatcgc taaggagctc ggcatcagcc agagcacggc
     6541 ctaccgagaa ctgcaggtcc ttgttgactt gcgactcgtg aacaagaagc gtcaggggaa
     6601 acgcacgctc acagaagagg gcgtggccgt tcttggcggc gtgttccaac cgtgatcgac
     6661 ggcaggttaa tcccgttcgg ccggagccgc gcgccaatcc tggaggttcc gcatggctgt
     6721 tgacaccaca tccgtcaccg gggtatggca ggctgctctc cgccgtattg aggggcggtt
     6781 gagtaaacca agctacgagt ctttcgtaaa agccatggcg cccatgtcac tcgagggcga
     6841 cgtctttacc ttctctttgc cgagcaggct cgccaaggaa tgggtcgaga cccgctacag
     6901 cggcctgatc cgggcagccc tccaggaggt actggcgcgg cccgtcggca tacagctggt
     6961 catcgccgag gaagtccctc cggcgccggc actacggcca ccccaacgaa tccctgacgg
     7021 cctccagctc agcccaaagt acacctttga tacgttcgtc atcgggtctg gcaaccggtt
     7081 cgcccacgcc gccgcgatgg ccgtcgccga ggcgccggcg cgcgcctaca acccgctctt
     7141 catctacggg ggcgtggggc tgggaaaaac tcacctcctc caggcgatcg gccaccacgt
     7201 gatccaccgc aagcaactac accgagtggc gtacatcagc agcgagaagt tcacgaacga
     7261 actgatcaac tccatccgtg acgaccgcac cctggagttc cgcacgaagt atcgaaacgt
     7321 cgacgtactg ctgatcgacg acattcaatt cctcgccggg aaggaacgaa cccaggagga
     7381 gttctttcat accttcaaca cgctccacga agccagtaga cagatcatca tcagcagcga
     7441 ccggccgccg aaggagattc ccacactcga ggatcgccta cgctcccgtt tcgaatgggg
     7501 actgatcgcc gatatccagc ccccggacgt cgaaacgcgc atcgcgatcc tccgaaagaa
     7561 agccgaggtc gacggcattg gcgttcccga cgaggtcgcg gagttcatcg cccagcgcat
     7621 ccaatccaac attcgtgagc tcgagggagc gctggtgcgc gtggtcgcct acgctaacct
     7681 tacccgctct accattactg ttgatctggc atctgaaatc ctgaaagagt tattgcctgt
     7741 ctcaccggcc cgcccgatta cgatcccctc cattcaaaaa gccgtcgcgg ctcactttgg
     7801 aatccgtgtc gaggaaatgc gggccaaacg ccgaacgaag ggtgtcgcat tcccacgcca
     7861 ggtcgccatg tatatggcgc gcgaactcac ggatgcttca cttccccgta ttggtgaaga
     7921 gtttgggggc cgagaccata cgacggtcat gcatgcctgc gaccgcgtca aggaagctct
     7981 aagccaggac tcgcatcttg tcgcaacgct caagagcgtc gttgataccc ttaagaccgg
     8041 tgagtagtgg gtatctgcct tagttatcca gacgtctgta cacacccggt tccggctatg
     8101 attgaaggcg caaaagtcat ccgtccacat tattcacagc actattacta ttactagtgt
     8161 ttaaataaca ataattagta ctaatgccgc cggaggttga tcgatgtggg taacttgctc
     8221 tcaatccgct ctgcttaaag gcgtacatct cgtcaaccgc gccatttcta cacgtaccac
     8281 catgcctatc ttgagttatg tcctcttgga gacaacaaaa agcggactga aactcacagc
     8341 tacagatctc gaactggcta ttcaggttga gatagatggg gaagtcaagc aaggcggaca
     8401 actgacgctg cctgcgcgga tcctgacaga aatcgtgggt aatttgccgg aatcgaccgt
     8461 tgaaataaag gcagacgaag gagtcaccca aacacaaatt acctgtgaga acagcaactt
     8521 tgagatcctt gggcttccgg ccggtgactt tccgacgatg cctaagcccg ctgaggatgc
     8581 actgggcgct attgacgccg agtcgatgcg gacgatgatc agacaaacca tttttgctgt
     8641 gtcgggggat gagacccggc cattcctaac gggcgtctat gttgtgatgg acgggaccga
     8701 tgtgcgtttt gtagcaacag atggaggcag gctggccctg cggaagacca cgctccctag
     8761 tcctgtcaac caaaaaaccg gggtcatcgt ccccgcaaag acgatgcaag aactggcacg
     8821 cgccctggga ggaatggagg gggacgtcca gctggctgcc gtggacaatc agctgatgtt
     8881 tgctgtcccg ggattacgcg tatattcgcg gctgatcacc gggcagtttc ccaactacca
     8941 gcaggtaatt ccacaagagt tcaaacagcg gatccgcgtt gacacagacc ggttgctgcg
     9001 cgccgtgcgg cgcgcgtcca tcaccgcacg agattcggcc aacgtcgtcc ggttggcggc
     9061 gaaggggaag aagctgacga tcacctcgaa tacgcccgag gtcggaaaag ctcgtgagga
     9121 tgtcgaggtc acggccgagg gcgataccgt ggaggccgcg ttcaacgcgc ggtatctcat
     9181 cgactgtctc aacaacattg acgcggccga tctctatttc gagcttacgg gaccgctcag
     9241 cccaggagcg atccggccgg cggagcacga ggactatgtg tacgtccttg ctcccgtgcg
     9301 cgtgtacgga tagacagaag gaccggagct cacgaacaag cgaacaggaa aggacgctgt
     9361 tcgcttgttg tgtttcatgg ctgctgaacg aaacgttgct atccgtactg agtccattga
     9421 actcgccgcg ctgctcaaat gggcgcaagc ggtgaacagc ggtggggagg ccaagcagct
     9481 gatccaggac gggcacgtac gggtcaatgg agaggtagaa taccgtcggg cacgcacggt
     9541 gaccatcggc gaccgtgttg agatcgatga catcgtgctg atagtgacca agcgctgatg
     9601 catcttactc acctccgtct catcaatttc cgcaatcatg cgcgcacgga gatcgaaccc
     9661 ggcccgcgtc tcaactgctt cgtgggacaa aatgcccaag gcaagtcgtc gatcctcgaa
     9721 gcggtagaga tcgcggccac cggccgctct caccgcgccg tccgcgatac agatctgctg
     9781 catttcgggg aagcatgggc gcgcgtgcga gccgccgtcc ggcgggggga tcgcgacgtc
     9841 gaggtcgata tcgtctggcg tacggagacg tccaccgcgg gggcgaagga atttcgcgtc
     9901 aacggagttc ccgttcggcg aggggatctc tttgggcatg tgctgatcgt tgcgtccaca
     9961 ccacatgacc aggaggtggt tttaggtgcg ccggcgcacc ggcggcggtt gctggacctg
    10021 ttgctgagcc aaatcagccc ggcgtacttc ttcttattgc tgaggtatgc gcgcgcttta
    10081 gagcagcgga atcgattgct gcgagatctg gccggccccg ctcgtgaacg cgaggccgcg
    10141 ctcgacccgt gggacgaaca gctcagcacg atgggtgcgg cgatcacggc ccgccggctc
    10201 gttatcgtga cgcggctttc ccaggccgct cgggaagcgt atgcttttct ggcgagcgac
    10261 caggaagcgg tgtcgatcgc gtacgcgcct tcgctgtcgg cgagtgatga gacagccatg
    10321 attacgatgg cgcgaggggc gttcgcgcgg cggcggcgcg aggaggtcat tcgagggacc
    10381 acgttgaccg gaccgcatcg ggacgacctc cgtatggaga tcgacgggcg cgaccttcgc
    10441 acttttgggt cccgagggca acatcaggcc gcgatgctgt cgctccggct tgcggagcga
    10501 aggatattgc tcgaggagag cggcgaagag ccggtcctgc tgctcgatga cgccgcacaa
    10561 ttgctggacg aagagcggca ggtacggctg atgcagttgg tcggccgagc gcaggcgtta
    10621 ctcacgttga cgacgaccgc ggcgctgcgg cacgtccccc cggacacgac cgtcttcaag
    10681 gttgcaggcg gccgcgtcga gcctcaactt gcttacccca cttagatcga tcttaaagac
    10741 cgcggcaaag tcgctgggcc tggaacgggc cgcgaacgcc gcgttcgttc aggaactctg
    10801 gcccgaggtc gccggccccg aggccgcagc ccacacgcgc gttcaggggt tgcgaggccg
    10861 gacgctgctg atcgatgctg aaccggggcc atgggctcag gacctcacgg cgcagcgcgc
    10921 caggtacgtg agagaattca acaggcgcct cggagaggat gttgtggaag agatccggtt
    10981 ccggcagcgc ccgctgacct gatatctaga tgtatgtcca cctgggcgga gacgtcgtgg
    11041 tcccggcgaa ggacgtcgtc gccattcttg atgcgcgcct gctcggacaa tctgaggtca
    11101 accgcgagct cgtagaacgc gcggtcacgg cgaagaaact tcatggccca gggataggtc
    11161 caggctgcaa ggcactggtg gtggcagtcg acgcggtgta cacgtcaggc atctcggcgc
    11221 ccacccttgc ccgccggatg gtccacttca ggcagtccgc gctttcgtgg gaggccgaaa
    11281 cgcaagtcta gatgcgggtt ttacgcgttg acgccttttc cgcaccgtgc tatactgaac
    11341 tgtgctgcat tttagcaccc ttgcacgtgc gtgtacggca gttttccagc ccgccagcga
    11401 gttttcagag gtccagacgg gcatatttct tgacagacca ccctttaata agaaggaggg
    11461 gacggatcga atttcgctga gcatctatgg aacgggagac gaacatggcc accaaccaaa
    11521 cgaccacccc ctacaccgcg gaacagatcc aggtcctcga agggctggag ggcgttcgca
    11581 aacgcccggc gatgtacatc ggaagcaccg ggcccgatgg tctgcaccat ctcctctacg
    11641 aggttgttga caactccgtc gacgaagcgc tggccggacg ctgcgatgag atagccgtta
    11701 ttctccacaa ggatgacagc gcgtccgtca tcgacaacgg cgccggcatt ccggtagatc
    11761 ccgttcctaa gatcggtaaa cccgctgttg aagttgtcct cggcacactc catgccggcg
    11821 gcaagttcgg cggtggtggt tataagattt ccggcgggct gcatggcgtc ggggtgtcag
    11881 tcgtcaacgc gctctccgag tggctggaag tcgaagtgtg gcgcgacggc aagacgtaca
    11941 agcagcgctt tgcgcgcggc aagaagacga caccgctcga gcaggttgga aacacacgca
    12001 agaagggcac gcgcgtcacc ttcagagctg atcccgaggt gatgacggcg atggagttca
    12061 atgcagaggt catcgccggc cgcctcgagg acattgcgta tctcaacaaa gggcttacca
    12121 tccgtctgca cgacgagcgt agcgataagg agcaagtatt caagcacgcc ggcgggattg
    12181 ccgaactcgt ccaggccctc aaccggacga agacgaaggt gtctgccgtt atgcagctga
    12241 cgaaagagcg tgacggcgtc gaagtcgaag tcgcgttgca gtacagcgag agttacctcg
    12301 agcatacgct gacctacgtc aacaccatcc cgaccacgga aggcggcact cacgtcgcag
    12361 ggttccgctc ggcgctgacc cgggcgatca acgactatgc acggcgcagc ggcctgctga
    12421 agaacggcga tgtgacgctc atgggtgacg atgtgcgtga gggtctgact gcggttctct
    12481 ccctgaagct cccagagccg cagttcgagg gacagacgaa gacgaagctc ggcaacaccg
    12541 aagtgaaggg gctcgtcgag tcggtcgtcg gcgactggct gcaagaatgg ctcgaaacac
    12601 atccgggtga cgccaaacgc gtggtagcca aggcgctgac tgcagcgcgt gcgcgtgagg
    12661 cggccaagca ggcgcgagat ctcgtgcgcc gcaagaccgc gctggaggtc tcgccgctgc
    12721 ctggcaagct tgccgactgc gtcgaaacgg atcctgagcg tgcagagatc tttatcgtgg
    12781 aaggcgaatc cgccggcgga tccgccaagc aggggcgcga ccgtcagttc caggcgatct
    12841 tgccgatcca gggcaagatc ctcaacgtcg agaaggcacg cgaagacaag atggtctccc
    12901 acgaggagat ccgggcgatc atcacggcgg tagggaccgg gttcggagag aagttcaact
    12961 tcgatgagcg ccggtacgac agggtcatca tcatgtgcga tgcggatgtt gatggaagcc
    13021 acatccgcac gctgttgctg acgttcttct accgatacat gcgtcagctg gtggaacacg
    13081 gcaaggtata catcgctcag ccgccgctgt acttggttag atctggcaaa gatcgccgct
    13141 acgcgtattc tgatgaggcg cgccaggtgg cggtcaagga atttgaagcg aaaggcggca
    13201 agcccgaggt ccagcgctac aagggcctgg cagaaatgaa ccccgagcag ttatgggaga
    13261 caacgatgaa ccccgagacc cgtacgctgc tacgggtcga gatcgaggat gcagcggccg
    13321 cggatgacat ctttacgaag ctgatgggtg aagaagtgga gccgcg
//
LOCUS       DBNGSB010000025         3553 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_477478, whole genome shotgun sequence.
ACCESSION   DBNGSB010000025 DBNGSB010000000
VERSION     DBNGSB010000025.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_477478"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /gene="lysA"
                     /locus_tag="AC159B_00730"
     CDS             <1..963
                     /gene="lysA"
                     /locus_tag="AC159B_00730"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HIR3376310.1"
                     /translation="PDEIDYGLRTGVGRFIVDNEYELDLLEAATARLGVDANILLRLT
                     PGIEPHTHKSIQTGGVDSKFGFGILDGAAKGAVDRARRIPRVRLRGLHAHIGSQGFDL
                     DPFRASATAIMDFAARIRDDFDVTVEELNLGGGLAIRYLRTDEPPSINDYVAALAQTV
                     KAKLVEHRMSPPTLFCEPGRSIVGDAGVTLYTAGAIKVIPGVRTYVSVDGGMFENPRP
                     ALYGARYEAVVATRPQQKPTQTVAVAGRCCESGDVLIWEAQLPDVKPGDLLATFSTGA
                     YTYSMASNYNRFPRPAVVLVGGGRARAVVERDTYDDMVRKDVAL"
     gene            982..1614
                     /locus_tag="AC159B_00735"
     CDS             982..1614
                     /locus_tag="AC159B_00735"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR3376311.1"
                     /translation="MLGLSFLSLSPLILLFRGLALIVAAPIHEFAHAYIADRLGDPTP
                     RSQGRLTLNPLAHLDLVGTLFVLLTGFGWAKPVQINPSHFSNWRRDIILVAAAGPLAN
                     VTALFILGLPIKLRLIDAQSLVGELVFMAIVINAGLALFNLLPIPPLDGSKILIGILP
                     PGPAIAYARLQPYGILLLFAVVFVFPGLVSGLLVQPMDWLVGQATGRSFF"
     gene            1623..2330
                     /locus_tag="AC159B_00740"
     CDS             1623..2330
                     /locus_tag="AC159B_00740"
                     /inference="COORDINATES: protein motif:HMM:NF014654.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="segregation and condensation protein A"
                     /protein_id="HIR3376312.1"
                     /translation="METYTIKLPQFEGPLDLLLSLAQQGQVDLLQIPLGQIAEDYLSA
                     TRGDIDLEQATEVLWTLAAMIEMKSRLLVPKPEPSEAVVAEDQASDLQERLEERLGEY
                     RSFKEVATALRALEDYQKRVFSRTTTGDPEAVVLEGVTIDDLFRAIQRVLERAREDVS
                     EIAAEEIKVADRMDTILALLAAHPEGVEFAALFGEASSTLEVIVTFLALLELIKDRRV
                     RAQQAGTFAPITLAVTS"
     gene            2327..2890
                     /gene="scpB"
                     /locus_tag="AC159B_00745"
     CDS             2327..2890
                     /gene="scpB"
                     /locus_tag="AC159B_00745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HIR3376313.1"
                     /translation="MTDIDTLKRAVECILLVGGGPVRLQQLRSALDAPDDVITAALSE
                     LARDFEGHGLQIQEVAGGYQLVTRPAYADYVQRFLQLEHQETLTRAQLETLAIVAYRQ
                     PVTRAEIDAVRGVRSDYVLDRLLDRYLIREVGRRPTLGRPILFGTTEAFLRHFGLRDL
                     SSLPPLANHDPRNALEAVAPQELSVSS"
     gene            2997..>3553
                     /locus_tag="AC159B_00750"
     CDS             2997..>3553
                     /locus_tag="AC159B_00750"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR3376314.1"
                     /translation="MLIDSRSGRVLFAKSADLRWSPASTTKILTAMLAMDALSDEALV
                     PISDRASVERSGSAIGLEPGERWKARDLLHALLLHSANDAAVALAEAVGGSVQQFAVY
                     MNARATTLGARRSRFVTPHGRYHPNHLTTAHDLAVIARAALHMPRFAAIVRTQTWDLF
                     RPGQKPRMVINTNSLLWRYRGADGV"
BASE COUNT          618 a         1128 c         1120 g          687 t
ORIGIN      
        1 ccggacgaga tcgactacgg gctccgaaca ggcgtcggcc gctttatcgt cgacaacgag
       61 tacgagctcg acctgctcga ggctgcgacc gcgcggctcg gggttgacgc gaacattctt
      121 ctacgcctca ctcccggcat cgagccgcac acgcacaaat cgattcagac cggcggtgtc
      181 gactcgaagt ttgggttcgg aatcctcgac ggggccgcga aaggcgctgt cgatcgagcc
      241 cgcaggattc cgcgcgtgcg ccttcgcggg ttgcacgcac acattggatc ccagggtttt
      301 gacctcgacc catttcgcgc gtcggcgacc gccatcatgg attttgcggc tcggatccgc
      361 gacgattttg atgtcacggt cgaagagttg aacctgggcg gcggcctggc catccgctat
      421 ctgcggacgg atgagccacc gtcaatcaat gactatgtcg cggcactcgc ccagactgtg
      481 aaggccaagc tggtcgagca ccgcatgagt ccaccaacgc tcttctgcga gccggggcgg
      541 tcgattgttg gagatgccgg cgtaacgctc tacactgctg gggccatcaa ggtgattccc
      601 ggcgtgcgca cgtacgtgtc ggtggacggt ggaatgttcg agaacccacg accggcgctg
      661 tatggcgcac gatacgaggc cgtggtggcc acccggccac aacaaaagcc aacgcaaacg
      721 gtcgccgtgg caggtcggtg ctgtgagtcc ggcgatgtgc tgatctggga ggcgcagttg
      781 ccggacgtga agcccggcga cctgctggcg acgttctcta cgggcgcgta cacgtactcg
      841 atggcgagca actacaatcg gttcccgcgc cccgcggtcg tgctggtcgg cggcggtcgg
      901 gcgcgcgcgg ttgtcgagcg ggatacgtac gacgacatgg ttcgcaagga cgtggccctt
      961 tagcccgcga accattttct gatgctgggc ctgtcgtttc tgtcgctcag ccccttgatc
     1021 ctcttgttcc gggggctggc tctaatcgtc gcagcgccga ttcatgaatt cgcccatgcc
     1081 tacatcgccg atcgtctggg tgatccgacg ccccgcagcc aggggcggct taccctcaac
     1141 cccctggcac acctcgatct tgtcgggacg ctcttcgtgt tgctgaccgg gttcggctgg
     1201 gcgaagcccg tgcagatcaa cccgtcgcat ttcagcaact ggcgccggga cataatcctg
     1261 gtggctgcgg ccggcccgct cgcaaatgtc acggcgcttt tcattctcgg tctccccatc
     1321 aagctcagat tgattgatgc tcaaagcctc gtgggagagc tggtgttcat ggcgatcgtg
     1381 atcaacgccg gacttgcgct cttcaacctg ctgccaattc caccgctgga tggatcgaag
     1441 atcctgatcg ggattctccc gccgggaccg gccatcgcgt atgcgcgcct ccagccgtac
     1501 ggcatcctgc tgttgtttgc cgtagtattt gtgttcccag ggctagtgag cgggttgctg
     1561 gtacaaccca tggactggct ggtcggtcag gcgacaggcc ggagtttctt ctgaggagag
     1621 acgtggagac ctatacgatc aaactgccgc agttcgaagg tccgctcgac ctgctgctct
     1681 cgcttgccca acagggtcag gttgacctac tccagattcc cctcggtcaa atcgccgaag
     1741 actatctttc cgctactcgc ggcgacattg acctcgagca ggccacggag gtgctgtgga
     1801 cgctggctgc gatgatcgag atgaagtcaa ggctgctcgt gccgaaaccc gagccatctg
     1861 aagccgtcgt tgcggaagat caggccagcg atcttcagga acggctggaa gaacgtctgg
     1921 gggaataccg gtcattcaaa gaagtggcca ccgcgctgcg ggccctcgaa gattaccaga
     1981 agcgcgtctt ctctcgcacg acgacgggcg accccgaagc cgtggtgctt gaaggcgtga
     2041 cgatcgacga ccttttccgt gcgattcaac gtgtgctgga gcgggcgcgt gaagacgtca
     2101 gcgaaattgc cgctgaggag atcaaggtcg ccgatcggat ggatacgatc cttgcgctgc
     2161 tcgcggccca cccggagggg gtcgagttcg cggcgctctt cggggaggca tccagcaccc
     2221 tcgaagtcat cgtgacgttc ctggcattgc tggaactcat caaagaccgg cgcgtccggg
     2281 cgcagcaggc gggcacgttt gcgccgatca cgctcgcggt cacatcatga cggatatcga
     2341 caccctcaaa cgcgctgtgg agtgcatcct cctggtcggc ggcggtcccg tacgactcca
     2401 gcagttgcgc agcgcgttgg atgctcccga tgacgtgatc accgccgcgc tgtcggagct
     2461 ggcgcgtgat ttcgagggtc acgggctgca gattcaggaa gtcgccggcg ggtatcaact
     2521 cgtgacccgg ccggcgtacg cggattacgt gcagcgattc ctgcagctcg agcaccagga
     2581 gacgctgacg cgcgcccagc tcgaaaccct tgccatcgtc gcctaccgcc agccggtgac
     2641 gcgcgccgag attgacgctg tccgcggcgt gcggtccgac tatgtgctcg acagactgct
     2701 cgaccgctat ctgatccgag aagtcggccg gcgtcctacg cttggccggc cgatcctgtt
     2761 tgggacgacc gaagcgttcc tgcgacactt tggattgaga gacctgagca gccttccacc
     2821 actcgccaac catgatcccc gcaacgcgct tgaggccgtc gctccccagg aactctcggt
     2881 ctccagttag cctgcgcctt gcagctgcgg cgctccttgc ttttgtacta ggaagcgctg
     2941 cctccgtccc ggctgcacca caggcgcgcc cgccgcgcgt cgacgccacg gctgccgtgc
     3001 tcatagattc gcgcagcggc cgcgtgcttt ttgctaagtc ggccgatctg cgctggtcac
     3061 ctgccagcac gacgaaaatc ctgacggcga tgctggccat ggatgcgctg tccgacgaag
     3121 cgctcgtacc gatcagcgac cgagcttcag ttgagcggtc agggtccgca atagggctcg
     3181 aaccagggga gcggtggaaa gcccgcgatc tgcttcatgc gctgctgctg cactcggcga
     3241 acgacgcggc ggtggcgctt gcggaagcgg tcggcggttc ggtgcagcag tttgccgtct
     3301 acatgaacgc gcgcgcgacc acattgggtg ccaggcgcag ccggttcgtc acgccgcacg
     3361 gccggtatca cccgaatcac ctgacaacgg cgcatgatct cgccgtgatc gcccgcgcag
     3421 ccctgcacat gcccaggttc gcggcaatcg tccgcacgca gacctgggat ctgttccgcc
     3481 cgggtcagaa accccgaatg gtgatcaaca cgaacagcct cctgtggcgc taccgcggtg
     3541 cggatggcgt aaa
//
LOCUS       DBNGSB010000026         9592 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_4158026, whole genome shotgun sequence.
ACCESSION   DBNGSB010000026 DBNGSB010000000
VERSION     DBNGSB010000026.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 9592)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9592)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9592
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_4158026"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..397
                     /locus_tag="AC159B_00755"
     CDS             125..397
                     /locus_tag="AC159B_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR3376315.1"
                     /translation="MATVRVPAPLRRLTSEQRIVTASGKTLAELVRDLDRQFPGFAAR
                     VLDNDGKILSFVNVFVDNEDVRFLQGLQTPVGNDTEVAIIPAMAGG"
     gene            427..2451
                     /gene="ligA"
                     /locus_tag="AC159B_00760"
     CDS             427..2451
                     /gene="ligA"
                     /locus_tag="AC159B_00760"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HIR3376316.1"
                     /translation="MGRRKTPLTKSQARKQVAEFRKAIDRHNYLYHVLDQPEVSDQEY
                     DRLFRQLQDLEREFPDLVTPESPTQRVGAPPSQVFAPVAHRQPMMSLANVFSEEELHA
                     WARRAQAALGSQTIKYVCELKFDGTAVSLTYENGAFVRGATRGDGVQGEDVTSNLRTI
                     RSIPLRLQAKRVPRVVEIRGEVFLSKSSFEAINRDRSAAGEPLFANARNAAAGSLRQL
                     DPKITASRPLDVFVYGLGAVDGLEVASHEAALAWMQSAGFKLNPNTAVAGTLEDVMTY
                     VTRWTQEREHLPFAADGVVVKVNDFRQQAELGATSQAPRWAVAYKFPAEQTETRINDI
                     KVYVGRTGTLTPVADLDPVGISGVTVTSATLHNEDEVRRKDVRIGDWVVVQRAGEVIP
                     EVVRVLTERRNGSERKFVMPKACPECGSPVYRPEGEAVTRCTNAACPAQVLGRLLLFG
                     SRGAMNIDRVGYKLVRQLLDRGMISDSADLYALTKVQLLTLERMGEKSAQNVLDSISS
                     SKKTTLSRLLYALGIRHVGSHVAEVVADHFGTVERLMQASFEEVRDVPGIGPTIARSI
                     AEFFRQPENRRIIKKLLAAGVRPAAPRKSEHRGPLAGKRVVFTGTLPHLPRGRAEALA
                     KERGAIVAGSVSSKTDYLVAGEDPGSKLEKARKLKVRVLDEDEFAKLTGS"
     gene            2455..2967
                     /locus_tag="AC159B_00765"
     CDS             2455..2967
                     /locus_tag="AC159B_00765"
                     /inference="COORDINATES: protein motif:HMM:NF022112.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HIR3376317.1"
                     /translation="MMRIVGIVLAVAVAAAVLGLWWLTRPTSTAVEVYFVRDEGTVST
                     VVPVSRTVQASGTLDLLDAALRELLAGPTEAERFSGLTTAIPAGTRLRSIGVERGVVS
                     ADFTREVESGGGSASMLGRFWQIVHTATQLRAAPRVRILIEGQQRESMGGEGVVIDRP
                     IERPATPPRF"
     gene            3125..4228
                     /locus_tag="AC159B_00770"
     CDS             3125..4228
                     /locus_tag="AC159B_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376318.1"
                     /translation="MASEIFAEDIEALLGRLEGVASARVVANDAGQVERIYVTAAADR
                     DETALRRMVGAALMSQYSLAVDGWRVRIARLRPQHDAPAGRWELSRMEELLSPTTSRL
                     TVQLRADNSRGRGLTGSAEGPTDQANRRKAAALATLDALKSVFEAEGWKPSIETITSV
                     PLGDGDAVVVGISLSSPVDSQLYVGTAVSEGNEAEAVIAATLEAIGKRTVQSDEGGWT
                     MKDRRDQLEAMRAHYRSRREVQRELPVLSETERSEPELEEDEHPPEEGTARLAQIRPE
                     RPGGAAVEGRSEPGRGGDQRSAPKGAMEDEYFRHLIATGIPIHFRCRDGYEITEAVLK
                     EFGTYTLLVETDDGRELIFKHAVISIHPMRLKE"
     gene            4383..5930
                     /locus_tag="AC159B_00775"
     CDS             4383..5930
                     /locus_tag="AC159B_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR3376319.1"
                     /translation="MPDDRLEDLRRRRERVKALGGAERVKRQHEAGKLTARERLEHLV
                     DPGSFTESGMFVTHRAREFGMDQVDAPADGVITGYGTIEGRLVFVFSQDFTVLGGSLG
                     EAHAGKIVKLMDAALRAGAPVIGLNDSGGARIQEGVASLGGYADIFLRNTLASGVIPQ
                     ISAVLGPCAGGAVYSPAITDFVVMVRKTSYMFVTGPQVLKAVTREDVGFEELGGADVH
                     STRSGVAHFVAADDLECLQLIRRLLSDLPQNNLDDPPHLEPSDDPARMDAELDRLIPD
                     DPTKPYDMHEVISRIIDTGSFLEVHTHYAQNLLVGFARLDGHTVGIIAQQPRILAGVL
                     DIASATKGARFIRFCDAFNIPLITFVDVPGFLPGVEQEHGGIIRHGAKLLFAYCEATV
                     PKLAVITRKAYGGAYDVMSSKHVGGDLNLAWPTAEIAVMGPEGAIEIVFRRELSDAKD
                     PDALRAKLVSEYRAQFANPYVAASRGYVDDVIAPRETRPRLISALRALSTKRIANPRK
                     KHGNIPL"
     gene            5968..6480
                     /locus_tag="AC159B_00780"
     CDS             5968..6480
                     /locus_tag="AC159B_00780"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3376320.1"
                     /translation="MKSVSVPNKPMELEYVSGEDKKFRDWLRRYREETVGEPPPDEWL
                     DAYLKHIFLEQGKNRHIWWGVDEGKKIGFAVAILTPLAPDRSKVQGMVAEFFIYPEYR
                     REGVGRRLAEAVIEFLHKQGANDVHASVTAGNVRGLRFWEACSFQISRYVLVHRPGLK
                     LEEEEEEEEL"
     gene            6505..7959
                     /locus_tag="AC159B_00785"
     CDS             6505..7959
                     /locus_tag="AC159B_00785"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR3376321.1"
                     /translation="MANRGEIAVRLVRACRELGFRAVAVYSPVDRRARHVLLSDEAYA
                     LPGDEPAASYLNSAALLEIASRAHIDALHPGYGFLSENPVFAAECQQAGVTFVGPPSA
                     VLAECGDKARTRERLTQARVPVLPGTSAVSDEEARSAAERVGFPLLIKASGGGGGKGI
                     HLVGSIGELPSTLRLARGEAKTAFGDDRVYLERWLPDARHIEVQILADTHGRISALGE
                     RDCSVQRRHQKLIEETPAPGLTPSMRRRLLEAAETGARMLGYVNAGTFEFLVHGDDYY
                     FLEVNARLQVEHPVTESVTGADIVVQQFRIARGKALDIELPISSRGHSIECRINAEDP
                     HEGFLPSTGTVGGLAEPNGPGIRVDSSLYPGMEVTRHYDPLLAKIIAWGTTRGDALAR
                     MRCALLETAVGGLATTVPFHLWALDDPVFAAGSHDTQFARRWDARKPGDDLPRALLAA
                     AAFEHIERGRVRFPPERRDGAWHRASKEEGLERL"
     gene            7946..8464
                     /locus_tag="AC159B_00790"
     CDS             7946..8464
                     /locus_tag="AC159B_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HIR3376322.1"
                     /translation="MSDYEVTSGPHRFVLRVEYADGGIQVTIDGRVHRVEMQRWVPPT
                     HFVMSVDGISTPVAVSRIQDELRVVVGWEQYRLQVAPRLPIQRRGATDRSGSTDIRIV
                     APMPGLIVAVQAGPGSQVEQGTPIVIMEAMKMQMEIRAPRAGRLSGISVKPGEEVAKG
                     QILATLKTEHGR"
     gene            8454..>9592
                     /locus_tag="AC159B_00795"
     CDS             8454..>9592
                     /locus_tag="AC159B_00795"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA mutase large subunit family protein"
                     /protein_id="HIR3376323.1"
                     /translation="MDDSERRAKFETPDGIEIRRVYNPGDVEPNYRRDIGFPGEYPFT
                     RGLHPTMYRGRLWTMRQYAGFGTAEESNARYRYLLSQGQTGLSIAFDLPTQMGYDSDH
                     PMAEAEVGMTGVAIDTLRDMEVLFRGLPLDTISTSMTINATASILLAMYVSVAEQQGV
                     ALSAISGTTQNDILKEYIARGTYIYPPGPSLRLITDVVTYCADHLPKWNPISISGYHI
                     REAGATAVQEVAFTLADGVTYLQAAGAAGLPVDRIAPMLSFFFAAQMNLLEEVAKFRA
                     ARRLWARVVQEKFGATDPRSMMLRFHTQTAGVALTAQQPNNNLVRVAIQALAAILGGT
                     QSLHTNASDEALALPTDESALLALRTHHIIAYENGVANNVDPLAGS"
BASE COUNT         1837 a         2904 c         3093 g         1758 t
ORIGIN      
        1 aggggagacc gaagcctttc cccgactgaa acggatacgg ggaccccttt ggctccggtc
       61 tcccctgcgc atcccgggca atctgcatac tgtttggcgt acgcattaga gatggagggc
      121 gggtatggca acggtacggg ttccggcgcc gctccgtaga cttacgagcg agcagcgcat
      181 cgtgacggca agcgggaaga cgctggcgga gctggtaagg gacctggatc gccagttccc
      241 gggattcgcc gccagggtgc tcgacaacga cggaaagatc ctcagcttcg tgaacgtttt
      301 cgttgataat gaggacgtga ggtttcttca gggtctccag acccccgtag gcaacgatac
      361 ggaggtcgcg atcatccccg cgatggcagg cggataaccc taaacggcgg tcgtaggtcg
      421 taggtcatgg gtcggagaaa aactcccctc acgaagtctc aggcccgcaa gcaggtcgca
      481 gagttccgca aggccatcga caggcacaac tatctttatc acgtactgga ccaaccggaa
      541 gtcagcgacc aggagtatga tcgcctcttc cggcaactcc aggacctcga acgggaattc
      601 cccgacttag tcacaccgga atcgccaacg caacgcgtgg gcgctccgcc atctcaggtc
      661 ttcgctcccg tggcacatcg ccagcctatg atgagcctgg ccaacgtgtt ttccgaggaa
      721 gagcttcacg catgggcgcg gcgcgcacag gcggcgctgg gatcgcaaac gatcaaatat
      781 gtctgtgagc tcaaatttga cggaacagcc gtttcgttga cgtacgagaa tggagcgttc
      841 gtgcgcgggg cgacgcgggg cgacggcgtt cagggggaag atgtcaccag caatcttcgc
      901 acgatccgca gcattccact ccgtctgcag gcgaaacgag ttccccgagt tgtcgagatt
      961 cgcggtgagg tgtttctgtc gaagagctcg ttcgaggcga tcaatcgaga ccgcagcgca
     1021 gccggcgaac cactttttgc caatgcgcgc aacgcggcgg ccggatcatt gagacagctc
     1081 gaccccaaga ttactgcctc gcggccgttg gacgtcttcg tctacgggct cggagccgtc
     1141 gacgggctgg aggttgcctc gcacgaagcc gccctggcct ggatgcaatc cgcgggattc
     1201 aaattgaatc caaacaccgc cgtcgccggg acccttgaag acgtgatgac ctatgtgacg
     1261 cggtggactc aggaaaggga gcaccttcct tttgctgcgg atggcgttgt ggtgaaggtc
     1321 aacgactttc gccagcaggc cgagctcggg gcgacgtcac aggcgcctcg ttgggcggtg
     1381 gcgtacaagt ttcccgcgga acagaccgaa acccgtatca atgacatcaa ggtatatgtc
     1441 ggccgcacgg ggacgctcac gccggttgca gatctcgatc cggtcggcat ctcgggagtg
     1501 acggttacaa gcgccaccct ccacaacgaa gatgaagttc gccgcaaaga cgtgcgcatc
     1561 ggcgactggg tggtcgtcca gcgcgcggga gaggtgatcc ccgaggtcgt tcgggtgctc
     1621 accgagcgcc ggaacgggag cgaacgaaag tttgtgatgc cgaaagcgtg ccccgagtgc
     1681 ggctcgcccg tctaccgtcc ggagggtgaa gccgtcactc gctgcacaaa cgcggcctgc
     1741 cctgcgcaag ttctcggacg gctcctcctg tttggttcac gaggcgcgat gaacatcgac
     1801 cgcgtcggct acaaactggt gaggcagctg ctcgatcgtg gtatgatttc cgactctgcc
     1861 gatctgtacg cactgacgaa agtgcaactc ttgacgctgg agcggatggg agaaaagtcc
     1921 gcacagaatg ttcttgactc aatctcctcg agcaagaaga cgacactctc ccgcctgctc
     1981 tatgcgctcg gcatccggca tgtcggcagc catgtggccg aggtggttgc ggaccacttt
     2041 ggcaccgttg aacggttgat gcaggcgtcg ttcgaagagg ttcgcgacgt gccggggatc
     2101 ggcccaacga tcgcgcggag tatcgccgag ttcttccggc agcccgagaa ccggcgcatc
     2161 ataaagaagt tgctggccgc gggcgtacgt ccggccgcac ctcggaagtc ggaacatcgc
     2221 gggccgctgg cgggtaagcg agttgtattc accggaacgc ttcctcactt gccgcgtggc
     2281 cgggctgaag cgctggccaa agagcgggga gccatcgtag ccggctcggt ctccagtaag
     2341 accgactatc tggtggccgg ggaagacccg gggtccaagc tggagaaggc tcgtaagttg
     2401 aaggtgcgcg ttcttgatga agacgagttt gccaagttga ctggttcata gacgatgatg
     2461 cgcatcgtcg ggatcgtgct ggcggtcgcg gtcgccgcag cggtactcgg cctgtggtgg
     2521 ctcactcggc cgaccagcac tgcagttgaa gtgtactttg tccgcgatga ggggacggta
     2581 tcgactgtcg tcccggtgag tcgcaccgtt caggccagcg gaaccctaga cctcctggat
     2641 gcagcgcttc gtgaactgct ggcgggaccg acggaggccg agcgtttctc cggtttgacc
     2701 acagcgatcc ctgccggtac ccgtctccgc agcatcgggg tggagcgcgg cgtcgtcagc
     2761 gccgacttca ctcgcgaagt ggaatccgga ggtgggagcg ccagcatgct gggacgattc
     2821 tggcagattg tgcataccgc tacacagctt cgggcagcgc ctcgggtccg gatcctcatt
     2881 gagggacagc aaagggagtc gatgggcggt gagggagtcg tgattgaccg gccgattgaa
     2941 cggccggcga cgccaccccg tttctgactc tttgttttga ccgttcgccg ttcccggttc
     3001 gccgctcccg gtatcgtcgc cgcaggaaaa tttcctcgtg tagtgaattt cttactaatt
     3061 gatctgcggg gcggactccg gggcccccgg gaaattcgcg ggcgcgaatt tctggggtgg
     3121 agacgtggca agcgaaatat tcgcggagga tattgaggcg ctgctcgggc ggctggaggg
     3181 cgtcgcttcc gcgcgcgtcg tcgccaacga cgcgggtcaa gtcgaacgga tctacgtcac
     3241 cgccgccgct gatcgtgacg aaacggcgct gcgccggatg gtaggcgcgg cgttgatgtc
     3301 tcagtactcg ctcgccgtcg acggatggcg ggtccggatc gcccggctcc gtccacaaca
     3361 cgatgctccc gccggccggt gggagctcag ccggatggag gagctgctca gccccacgac
     3421 ctcgcgtctg acggtgcagc tgcgggccga taacagccgc ggccgcggcc tcacaggcag
     3481 cgccgaagga ccgacggatc aggccaaccg ccggaaggcg gccgccctcg caaccctcga
     3541 tgcgcttaaa tccgtgttcg aagctgaagg ctggaagccc tcaattgaaa ccattacgtc
     3601 ggtccccctc ggtgatggcg acgcggtcgt cgtagggatc tcgctgtcat cacctgtgga
     3661 ttcacaactg tatgtcggca cggccgtttc cgaaggcaac gaagccgaag ccgttatcgc
     3721 agctacgctt gaagcgatcg gcaaacgcac tgttcaatct gatgaaggag gctggacgat
     3781 gaaggatcgg cgcgatcagc tcgaggcgat gcgtgcacat taccgcagcc ggcgagaagt
     3841 gcagcgcgag ctgcctgtct tgagcgaaac agaacgatcg gaacccgagc tcgaagagga
     3901 tgaacaccca ccggaggagg ggacggcccg tctggcgcag atcaggccgg agcgccctgg
     3961 aggcgccgcg gtggaggggc gatccgagcc tggccgcggt ggggaccagc gctccgctcc
     4021 gaagggagcg atggaggacg agtatttccg tcacctcatc gcgacaggta ttcctatcca
     4081 tttccggtgc cgagacggct atgaaattac ggaagcggtg ctcaaggaat ttgggacgta
     4141 cacgctgctc gtcgagaccg atgatgggcg tgagctcatc ttcaaacacg ccgtgatttc
     4201 gattcacccg atgcggttga aagaatagcg gggaaggaat cgagcagttc cgaaatagga
     4261 ccggggaccc ccgggaccgc ggaccagaag atcggctatg gagtgactta gctactggtc
     4321 ctcttgtccc gggggtcccc ggtccctttt catgcccgaa tcctgtagga aggtgaacct
     4381 gcatgcccga cgatcggctt gaggatctgc ggcggcggcg cgagcgcgta aaggccctgg
     4441 gaggggccga gcgcgtgaaa cgccagcacg aggccggcaa actcacggcg cgcgagcggt
     4501 tggagcatct ggtcgatccc gggagcttca ccgagtcggg gatgttcgtg acgcaccggg
     4561 cgcgagagtt cggcatggat caggtcgacg cgccggccga tggcgtgatc acaggatatg
     4621 ggaccatcga gggccgcctc gtctttgtgt tctcgcagga ttttaccgtg ctggggggat
     4681 cactcggcga ggcccacgct gggaagatcg tcaagctgat ggacgcggcg ttgcgcgccg
     4741 gggcgccggt gatcgggttg aacgactccg ggggagcccg aattcaggag ggcgtggcca
     4801 gcctcggcgg gtatgcggac atcttcctgc gcaacaccct tgcctccggc gtgattcctc
     4861 agatctcagc cgtcctcggt ccctgtgcgg gcggcgctgt ctactctcca gcgatcacgg
     4921 actttgtggt catggtgcgc aagacaagtt acatgtttgt cacgggacct caggtgctca
     4981 aagcggtgac gcgtgaagac gtcggattcg aggagctcgg cggcgccgac gtccactcca
     5041 cccggagcgg cgtggcccac tttgtcgccg ccgacgatct cgaatgcctg caactgatcc
     5101 gccgcctgct ttctgacctg ccgcagaaca acctcgacga cccgccacat ctggagccga
     5161 gcgacgaccc cgcgcggatg gatgcggaac tcgaccgcct catcccggat gatccgacaa
     5221 aaccgtacga catgcacgag gtgatcagcc gcatcatcga cactggatcg ttcctcgaag
     5281 tccacacaca ctacgcgcaa aacctccttg tgggctttgc ccggctcgac ggtcacaccg
     5341 tgggcatcat cgcgcagcag ccccggatcc tcgccggcgt gctcgatatc gcatcggcga
     5401 ccaagggcgc gcggttcatt cgattttgcg acgcgttcaa cataccgttg atcacctttg
     5461 tcgacgtccc gggattctta ccaggcgtgg agcaggaaca cggcgggatc atccgccacg
     5521 gcgcgaagct gctcttcgcc tactgcgaag cgaccgtgcc gaagctcgcg gtgatcacgc
     5581 ggaaggccta cgggggcgcg tacgatgtca tgtcgagcaa gcacgtgggc ggcgatctca
     5641 atctcgcctg gcccacggcg gagattgcgg tgatggggcc cgaaggcgcg atcgagatcg
     5701 tcttcaggcg agaactgtct gacgcgaaag accccgatgc gctgcgggcc aagctcgtct
     5761 ccgagtatcg cgcccagttc gccaatccgt acgtcgcggc ttcgcgcggc tacgtcgacg
     5821 acgtcatcgc gccgcgggaa acgcggccac gcttgatctc agcgctccga gccctctcga
     5881 caaaacggat tgctaatccg cgcaagaagc acggcaacat tcccctctaa agctcccttc
     5941 tttccgaagg aagaggtcct cgcgtagatg aaaagtgtaa gtgttcccaa caaaccgatg
     6001 gaactcgaat acgtttctgg tgaggacaag aagtttcgcg actggcttcg gcggtatcgc
     6061 gaagagacgg tgggtgagcc gccgcccgac gagtggctcg acgcctatct caagcacatc
     6121 ttcctcgagc aggggaagaa ccggcacatc tggtggggcg tggatgaagg gaaaaagatc
     6181 ggatttgcgg tggccatcct taccccgctg gcgcccgaca gatcgaaggt ccagggcatg
     6241 gttgccgagt tctttatcta tcctgagtac cgccgtgagg gggtcggccg ccgcctcgcg
     6301 gaggctgtaa tcgagttcct gcataagcag ggcgcgaacg acgtccacgc ttcggtgacc
     6361 gccggcaacg tccgcgggct gcgtttttgg gaggcctgca gcttccagat ctcacgctat
     6421 gtcctcgtgc accggcccgg actgaaactg gaagaggagg aagaggaaga ggaactctaa
     6481 accttcggta tctactccgt tctcatcgct aatcgaggag agatcgctgt ccgtcttgtg
     6541 agggcttgcc gcgagcttgg ctttcgcgcg gtggcggtct attcgcccgt ggaccggcgc
     6601 gcgcgacacg ttctgctctc cgacgaagcc tatgctctac ccggcgatga gccggcggca
     6661 tcctatctga acagtgccgc cttgctcgag atcgcaagcc gggcgcacat agatgcgctg
     6721 catccgggct atggatttct ctcggagaat cctgtcttcg ctgcggagtg ccagcaggcg
     6781 ggcgtgacgt ttgtcggtcc gccttcagcg gtgctggcag aatgcggcga caaagcccgc
     6841 acacgggaac ggctgaccca ggcccgcgtt cctgtactcc cgggcaccag tgctgtttcg
     6901 gacgaggaag cacgctccgc ggctgagcgt gttggctttc cacttctcat caaagcgtcc
     6961 gggggcggtg gaggcaaagg aatccatctc gtcggctcca tcggagagtt gccatccacg
     7021 ttgcgtttgg cccgaggcga ggccaagacc gctttcggtg acgatcgtgt gtacctggag
     7081 cgctggctcc cggacgcccg ccatatcgag gtgcagatcc tcgcagacac gcacgggcgc
     7141 atctccgcgc tcggagaacg cgactgctcg gtgcagcgcc gtcatcagaa gctcattgaa
     7201 gagactccgg cacccggact cacaccttcg atgcgacgcc ggctgctcga agcggctgag
     7261 accggcgcca gaatgctcgg ttacgtgaac gcggggactt tcgagttcct ggtgcacggt
     7321 gacgactact atttcctcga ggtgaacgca cggctgcagg tcgagcaccc cgtgacggag
     7381 tcggtgacag gcgcggatat cgtcgtccag caatttcgca tcgctcgagg gaaagccctg
     7441 gatatcgaat tgcccatctc gtcacgcggc cattctattg agtgccggat caacgcagaa
     7501 gatcctcacg aggggttctt gccgtcgacg ggaaccgtcg gcgggctcgc cgagcccaac
     7561 ggacccggca tccgggttga tagctccctc tacccgggaa tggaggtcac gcgccactac
     7621 gaccccctgc tggcgaagat cattgcctgg ggaaccaccc gcggcgacgc cctcgcgcga
     7681 atgaggtgcg ccctcctcga gaccgcggtt ggcgggcttg cgacaacggt gccctttcat
     7741 ctctgggccc tggatgatcc ggtattcgcc gccggtagcc acgacactca gttcgcgcga
     7801 cgatgggacg cacgcaaacc gggggacgac ctgccgcgcg cccttctcgc cgcggctgca
     7861 ttcgagcaca tcgagcgggg ccgagtccgc tttccgcctg agcgacggga cggagcctgg
     7921 caccgtgcat caaaggaaga gggccttgag cgattatgaa gtgacgtccg gtcctcaccg
     7981 ttttgtgctg agggtggagt acgccgacgg tggaatccag gtgaccatcg atgggcgcgt
     8041 ccaccgtgtc gaaatgcagc gctgggtccc gccgacacat ttcgtcatgt ccgttgacgg
     8101 tatatcgaca cccgtggctg tctcgcgcat ccaagatgaa ctgagggtgg tggtcggatg
     8161 ggagcagtac cgactccagg ttgctccgcg gcttcccatt caacgcagag gagccaccga
     8221 ccgatcgggt tcaaccgaca tacgcattgt cgccccgatg ccgggcttga tcgtggccgt
     8281 gcaggcgggt ccagggtccc aggtagagca gggcacgcct atcgtgatta tggaggcgat
     8341 gaaaatgcag atggagatcc gcgcgccgcg cgcaggtcgc ctctcgggca tctcagtaaa
     8401 gccgggggag gaggtcgcga aggggcagat acttgctaca ctcaaaacgg aacatggacg
     8461 atagcgagcg gcgagccaaa ttcgagactc cggatgggat tgagatccgc cgggtctaca
     8521 acccaggcga cgtcgagccc aactaccggc gcgacattgg cttcccgggt gaatatccgt
     8581 tcacgcgcgg actccacccg acgatgtacc gcgggcgctt gtggacgatg cgccagtacg
     8641 ccggcttcgg caccgcggaa gaatccaatg cccgctaccg ctacttgttg agccagggcc
     8701 agaccggact ctcgattgcg ttcgatctcc cgacgcagat gggctacgac tccgaccatc
     8761 cgatggccga agccgaggtg ggtatgaccg gcgtggccat cgacacgcta agggatatgg
     8821 aagtgctgtt tcgcggcctt ccactcgaca cgatctccac gtcgatgacc atcaacgcga
     8881 ccgcgtcgat cctcctcgcg atgtacgtct cggtggcgga acagcaaggc gtggccctca
     8941 gcgcgatttc gggcacgacg cagaacgaca tcctcaagga gtacatcgcc cgagggacct
     9001 atatctatcc gcccgggcca tcgttgcgcc tgatcaccga tgttgtcacc tactgcgcgg
     9061 atcacttgcc gaagtggaac ccgatttcga tcagcggcta ccacatccgt gaagccggag
     9121 ccacggccgt gcaggaagtg gcgtttaccc tggccgacgg cgtgacctac ctgcaggcgg
     9181 ccggggccgc ggggttaccc gtcgatcgca tcgccccgat gttgtcgttc ttcttcgcgg
     9241 cgcaaatgaa cctcttagag gaagtggcga aatttcgggc ggcgaggcgc ctgtgggcga
     9301 gggtcgtgca agagaagttt ggggcgaccg accctcgatc catgatgttg cgattccata
     9361 ctcagactgc cggcgtcgcg ctcaccgccc agcagcccaa taacaacttg gtccgcgtag
     9421 caattcaggc tctggccgcc atcctcggcg gaacccagtc cctgcacacc aacgcaagcg
     9481 acgaagccct ggccctcccg accgatgagt cagcgctgct ggccctcagg acgcatcata
     9541 tcatcgctta tgagaatggg gttgccaaca acgtagatcc cctggccggt tc
//
LOCUS       DBNGSB010000027         3349 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_4004442, whole genome shotgun sequence.
ACCESSION   DBNGSB010000027 DBNGSB010000000
VERSION     DBNGSB010000027.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_4004442"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="AC159B_00800"
     CDS             complement(<1..427)
                     /locus_tag="AC159B_00800"
                     /inference="COORDINATES: protein motif:HMM:NF019342.7"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HIR3376324.1"
                     /translation="MRGGAPRVGITANDPSTQSARRYASAVEAAGGVPRWITPEDLRA
                     AGERAALRNIDALLLSGGRDIDPRMYAESLTPGLDVDVDANRYEVEIPLGRAVLEERM
                     PVLGICGGMQVLNVASGGSLYQDISVLGIDPAVHRVQGRD"
     gene            complement(424..1149)
                     /locus_tag="AC159B_00805"
     CDS             complement(424..1149)
                     /locus_tag="AC159B_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376325.1"
                     /translation="MILRWAIWLVVGVVVSSLAAPAAGQSEADLRKLFAGFLKTNDLK
                     IDAVPDLYPGGFARISVYARKASLGGMQVDEVWFRVVGASLNVDAMQRGELRVDSWRE
                     SAIHARFTLRSLQEYFVSGNAFKDIRLWSDGEFLFGEGTVPLNNLPVRVWLKGWFALG
                     GTKDLYFYINNMRVNGVPLLDPIIRTLEAKYNPVLTQSTWPVTFDIRSLKVTKEVVVV
                     SSQTDGNAPCAFCSGGDVPIVSP"
     gene            complement(1186..2178)
                     /locus_tag="AC159B_00810"
     CDS             complement(1186..2178)
                     /locus_tag="AC159B_00810"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HIR3376326.1"
                     /translation="MQLGDLPRVKLAHLPTPLEFAPRLTEYLRGPKIWIKRDDLTGLA
                     MGGNKARKLEFLLGQAQSQGCDVVITVGAVQSNHARMTAAAARRLGLEAIVVLNGDAP
                     GERQGNLLLDHIFGAEVRVIQTDEEYVLMGVVEDMARQLRHDGRRPYVIPRGGSNAFG
                     AAAYMEAAFELLSQANAVSLRVDAIVHASTSGGTQSGLYTGTKLTQSGAQVVGISAGP
                     PREIVVKRVVGIVTELSEMLRLEWRPHPDDVIVYDDYVGERYGIPTKECLDAIRVVAR
                     TEGILLDPVYTGKAMAGMMGLIAQDRFRSDQNVVFWHTGGQPALFAHAPALQEG"
     gene            complement(2216..>3349)
                     /locus_tag="AC159B_00815"
     CDS             complement(2216..>3349)
                     /locus_tag="AC159B_00815"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit C family protein"
                     /protein_id="HIR3376327.1"
                     /translation="ADALSGIYPLQTPGGWRLIGRTPLRTYDPSKADPILFRPGDRVR
                     FIRVEHAEFPAGDAGPSLRTSSHAAFEVLEPGLFTTVQDLGRPGYRNLGMPASGAIDQ
                     DALRAANIMVGNEPGAPAIECTAPGPALRMRDEHWVVVAGADLAATLDGTEMQLWRPV
                     RARSGQEIRFGAPRHGMWAYVALAGGVDAGTVLGSASVYATGRIGRRLARGDILGAGA
                     RRVIGRPVNARLPAGVMRFPSDEIRLRVIPGPQESWFADGAESFYDSIFVVTVQSDRA
                     GLRFDGPAVPRREKEMLSDAMLPGAIQIPPDGKPIVILADGPTTGGYPKIGVVVSVDL
                     PLLGQARPGTRVRFERTTVEAAVNALRSREALFAELRDKGRKG"
BASE COUNT          632 a         1139 c          957 g          621 t
ORIGIN      
        1 cgtcgcgccc ttggactcga tgcacggctg gatcgatccc gaggacggag atgtcctggt
       61 agagactgcc acccgaggcg acgttcaaaa cctgcatccc tccgcaaatc cctagaacag
      121 gcatccgctc ctcgaggaca gcacgtccca atgggatctc cacctcgtac cggttcgcgt
      181 caacgtcgac gtcgagcccc ggggtgaggc tctccgcata catccgcggg tcgatgtccc
      241 ggccgccgct caacaagagg gcgtcgatgt tccgcagtgc ggcgcgttca ccggccgcgc
      301 ggaggtcctc cggcgtgatc cagcgtggta ctccaccggc ggcttccacg gccgacgcat
      361 agcgccgagc cgactgcgtg ctgggatcgt ttgccgtgat gccaacgcgg ggcgcgccgc
      421 ctctcacggc gatacgattg ggacgtcgcc gccactgcaa aacgcgcatg gcgcgttccc
      481 atcggtctgt gaagacacga cgacgacctc cttcgtgacc ttgagtgacc tgatgtcaaa
      541 tgttacaggc cacgtcgatt gcgtcaacac cgggttgtac ttggcttcga gggttcggat
      601 gatggggtcc agcaggggga cgccgttgac gcgcatgttg tttatataga agtagagatc
      661 tttcgtcccg ccgagcgcaa accatccctt cagccagacg cgcacgggga ggttgttgag
      721 cggaacagtc ccttctccga agagaaactc tccatccgac cagagacgga tgtctttgaa
      781 ggcgtttccg gagacgaagt actcttgcag gcttcgcagc gtgaaccgcg cgtggatcgc
      841 ggattctcgc catgaatcga cgcgcaactc tccgcgctgc atcgcgtcga cgttcagaga
      901 cgcgcccacg acacggaacc atacttcatc gacctgcatg ccaccgagac ttgccttccg
      961 tgcatagaca ctgatccgcg cgaagccgcc cgggtagagg tcgggaaccg cgtcgatctt
     1021 gaggtcattc gtcttgagga atcccgcgaa cagcttccgc aggtcggctt cggattgacc
     1081 ggcggcaggc gccgcaagcg agctgaccac cacgccgacc acaagccata ttgcccaccg
     1141 caatatcata cgttgcacat cgccttcctc accgaagtac acccactatc cttcttgaag
     1201 cgccggcgcg tgcgcgaaca gcgccggctg cccccccgtg tgccagaaca cgacgttctg
     1261 gtcggaacga aaccggtcct gcgctatcag acccatcatt cctgccatag cttttcctgt
     1321 gtacacggga tcgagcaaga tgccttccgt gcgagctacg acccgaatcg cgtcgaggca
     1381 ctccttcgtt ggtataccat accgttctcc cacatagtcg tcgtagacga tcacgtcgtc
     1441 gggatgcggc cgccactcga gcctcaacat ttcggacaat tccgtgacga tccccaccac
     1501 gcgcttgacg acgatctccc gaggcggtcc tgcgctgatc ccgaccacct gcgcgccgct
     1561 ttgagtgagc ttcgttccgg tatagagtcc cgattgcgtg ccgcccgacg tgctggcatg
     1621 cacgatggcg tcaactcgca ggctcacggc gttggcctgg ctcaggagct caaatgcggc
     1681 ctccatatag gccgccgcgc cgaacgcatt gctcccgccg cgaggaatga catagggcct
     1741 gcggccatcg tggcggagct gccgtgccat gtcttcaacg actcccatga gcacgtattc
     1801 ttcatcagtc tggatcacgc gcacctcagc gccgaagatg tgatccagca agagattacc
     1861 ctgccgctct ccaggcgcgt cgccgttgag caccacgatc gcctcaagcc cgagcctgcg
     1921 cgcagcggcc gccgtcatcc gggcgtgatt cgactggacc gcgcccaccg taatgacaac
     1981 gtcacaaccc tgactctgcg cctgccccaa taagaactca agctttcggg ccttgttccc
     2041 gcccatcgcg agtccggtga gatcgtcgcg cttaatccag atctttggcc cgcggagata
     2101 ttcagtcagc cgcggcgcga actccaacgg cgtgggaagg tgtgccaact tgacacgggg
     2161 cagatcgcct agctgcacgc tcaaccctca actgcggcgc atttacctta tccctttatc
     2221 cctttctccc tttatcccgt agttccgcaa agagcgcttc ccgcgatcgg agagcattca
     2281 cagcggcttc cacggtcgtt cgttcaaagc gcacgcgtgt gcccggccgc gcctgtccca
     2341 gtagcggaag atcgaccgac actacgactc cgatctttgg ataaccgccc gtggtaggtc
     2401 cgtcggcgag gatcacgata ggtttaccat ccggcggaat ttgaatcgcg ccgggaagca
     2461 tcgcatcgga aagcatctcc ttctcgcggc gcgggaccgc agggccatcg aagcgaagcc
     2521 cggcgcggtc gctctgcacc gtgacgacga agatagagtc gtagaaactc tcggccccat
     2581 cagcgaacca cgactcctgc ggtccgggga tgacacgaag acggatctcg tctgatggga
     2641 atcgcatgac gcctgccggt agtctggcgt tcacgggtct tccgatcaca cggcgcgcgc
     2701 ccgcgcccaa aatgtctccc ctggccagtc gccgtccgat cctgcccgtg gcatacaccg
     2761 aggcgcttcc aaggacggtc ccggcgtcaa ccccaccggc gagcgcaacg tacgcccaca
     2821 ttccgtgtcg cggcgcgccg aagcggatct cttgtcctga gcgcgcccgc accggccgcc
     2881 acaattgcat ctccgtccca tcgagcgttg cggcgaggtc tgcacccgcc acaaccaccc
     2941 aatgttcatc acgcatcctc agggcgggtc ccggcgccgt gcattcgatc gcgggtgcac
     3001 cgggctcatt ccctaccata atgttcgcgg cccgtagagc atcctgatcg atcgcgccgg
     3061 aagcgggcat ccccagattg cgataccctg gacggccgag atcctgcacg gtcgtgaaga
     3121 gcccgggttc gagcacctcg aaggcagcgt gcgatgacgt ccggagagat ggaccggcat
     3181 ctcccgccgg aaactccgca tgctccaccc gaatgaaccg gacgcggtcg cccggtcgga
     3241 acaggattgg atctgccttt gatggatcgt acgtccgcaa gggtgtccgg ccaatcaatc
     3301 tccacccacc cggagtctgc agtggataga tcccgctcag tgcgtcggc
//
LOCUS       DBNGSB010000028         2900 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_4158064, whole genome shotgun sequence.
ACCESSION   DBNGSB010000028 DBNGSB010000000
VERSION     DBNGSB010000028.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_4158064"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..1091)
                     /locus_tag="AC159B_00820"
     CDS             complement(204..1091)
                     /locus_tag="AC159B_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3376328.1"
                     /translation="MKMTTNRAALARSAVLAAALIVGCGPRDESAKRETAVTPGKYAD
                     VNGLRMYYEIHGTGRPLVLLHGGLAYIESSFGKMLPSLAKNRQVIGIEQQGHGHTADI
                     DRPLTYEQMAEDTAELLRRLGITAADFFGWSDGGVIATRIAMRHPELVRKFAILGTHY
                     NNGGIPPELRKAAANMSPEDVPAEFRAAYTKVAPKPEQWPTLVGKVIKLMDDSVWQGW
                     RPEDIRSIRAPALVMIGDADQVVRLDHIVQFFHLIPHAQLAVLPGTDHFAPLTRPDWI
                     LQMLEAFFDAPMPERKKAS"
     gene            complement(1229..2065)
                     /locus_tag="AC159B_00825"
     CDS             complement(1229..2065)
                     /locus_tag="AC159B_00825"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HIR3376329.1"
                     /translation="MIVHLLDGTYELFRHFYGLRRFNQGKDRPFGAVVGVLHTVLQMI
                     ETGATHVGVATDHVIESFRNDLWSGYKTGDGIEPALLAQFHPLEEALTAMGVAVWPMI
                     ELEADDALASAAEIASGDEDVEKVCIWTPDKDLAQCVRAARVVQVDRRGRKILDAEGV
                     RQKFGVEPSLIPDFLALVGDAADGYPGIPGIGRTTSARLLNQYGIIEDFPPAVLGDHR
                     DRALLFKDLATLRTDAPLFREVDELRWRGPTDAFAAYGERLGDARLLPRCLKAPPAVS
                     ST"
     gene            2150..>2900
                     /locus_tag="AC159B_00830"
     CDS             2150..>2900
                     /locus_tag="AC159B_00830"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HIR3376330.1"
                     /translation="MSTQLNAKAFPIAPPIEPMLAKLADALPVTGTFLFEPKWDGFRA
                     IVFRGASDVYIQSRDLRPFDRYFPELHEALLEKLPKGCVIDGEIVITTPQGLDFDALQ
                     LRLHPAASRVAKLAKETPASFVGFDLLASDGQSRMAAPQSERRALLERLLGDIGPPVY
                     LTPMTRDRGTATRWLDQFEGAGLDGVIAKLADSPYQPGKRAMIKVKHARTAECAVAGF
                     RWHKSGKDAVGSLLLGLYDDRGVLQHVGVTSS"
BASE COUNT          507 a          911 c          920 g          562 t
ORIGIN      
        1 caaagagttg ataagtcctg cgccccagga aaaccccgtc gatcgatttc agcatgtcgg
       61 tggcgtagcg gtcgaagtcc tggtcctcga accccgcagt aaagtccagt tcgcggttgg
      121 gtccttccat gaagccgtca agcgtcacgt tcatttgcca gaacagtgtt ctcatggctt
      181 gattcctatt cattgggtct aggctaggaa gcttttttcc gctccggcat gggcgcgtcg
      241 aaaaatgcct ccagcatctg caggatccaa tcaggacggg tcaggggcgc aaagtggtct
      301 gtgcccggga gcaccgcgag ctgggcatgt gggatcaggt ggaagaactg gactatgtgg
      361 tcgaggcgga caacctggtc ggcatcgcca atcatgacca gggcgggcgc ccggatggac
      421 cgaatgtcct ccggccgcca cccctgccag acgctgtcgt ccatcagctt tattactttg
      481 ccgaccaggg tcggccactg ttccggtttc ggcgccacct tggtgtaggc cgctcggaat
      541 tcagcaggaa cgtcttcggg gctcatattg gcagcggcct tccgaagctc gggtggaatt
      601 ccgccattgt tgtagtgagt cccgagtatc gcgaatttgc gcaccaattc cggatgccgc
      661 atggcgatcc gggtggcgat gacgccgcca tcgctccagc cgaagaaatc ggcagctgtg
      721 atgcctaggc gccgcagaag ttcggccgtg tcctccgcca tctgttcgta ggtcaatgga
      781 cgatcgatgt cggccgtgtg cccatgtccc tgctgctcga taccgatcac ctgccggttc
      841 ttcgccaggg aaggcagcat ctttccaaat gaggattcga tgtaggccaa gccgccgtga
      901 agcaggacca gcggcctccc ggtgccgtga atctcgtagt acatcctgag accgttcacg
      961 tcggcatatt tgcccggtgt cacagcggtc tcccgtttgg ctgactcgtc tcgcggtccg
     1021 catcccacga tgagtgccgc ggcgagcacg gcggatctgg ccagcgcggc cctgttcgtg
     1081 gtcatcttca tcgtgtcatc tccgaatcgc atcgtttcgc cctgattggg agccgggact
     1141 tgcagacggt ctcgaccggc tggacctggg ttctacttac ccgtcgaacg gagcgcggcg
     1201 aaatcgacac ctcaaagatg tttcttggtt aagtgcttga aacggcagga ggtgccttga
     1261 ggcaacgggg cagcaggcgg gcatctccga ggcgctcccc atacgcggcg aatgcgtcgg
     1321 tggggccacg ccagcggagc tcgtccacct cgcggaagag gggcgcgtca gtcctgagcg
     1381 tggccagatc cttgaatagg agcgcccggt cgcggtggtc tcccaggacg gcaggcggga
     1441 agtcctctat gatgccgtat tggttgagca gccgtgccga ggttgtccga ccgatccccg
     1501 gaatgccggg atatccgtct gccgcgtcgc cgacgagggc gaggaagtcg gggatcaacg
     1561 agggttccac cccgaacttc tggcgaactc cttccgcatc gaggatcttc cgccctctac
     1621 ggtcgacctg caccacccgg gcggcccgga cgcactgtgc caggtccttg tcgggtgtcc
     1681 agatgcagac tttttccacg tcctcgtctc ccgaggcgat ctccgccgcg gaggccagcg
     1741 cgtcgtcggc ctccagctcg atcatgggcc ataccgccac tcccatcgcg gtgagggcct
     1801 cttccagcgg gtggaattgc gcgagaagcg cgggctcgat gccatccccg gtcttgtagc
     1861 ccgaccaaag gtcgttccgg aaagactcga tgacatgatc cgtcgccacg ccgacgtgcg
     1921 tcgccccggt ctcgatcatc tgtagcacgg tgtggagcac gccgacaacg gcaccgaagg
     1981 gccgatcctt gccctggttg aagcggcgaa ggccgtagaa gtggcggaac agctcgtagg
     2041 tgccgtctag gagatgaacg atcatccgat agtatacggt gtcacgtcga gaagcgtttc
     2101 gcccatgata actttaggtt cccttaacgt gccccattcg ataggcgaca tgagcacgca
     2161 actcaacgcc aaagcctttc cgatcgcccc gccgatcgag ccgatgctcg ccaagctggc
     2221 cgacgccctg cccgtcaccg gcacgtttct cttcgagccg aaatgggacg gatttcgcgc
     2281 catcgtcttt cgcggtgcat ccgacgtcta cattcagagc cgggacctgc ggccgttcga
     2341 ccgctatttc ccggagctgc acgaggcgtt gctcgagaag ctgcccaaag gctgcgtgat
     2401 cgacggcgag atcgtgatca cgacgccgca ggggctcgat ttcgatgcgt tgcagctgcg
     2461 gctgcatccc gcggcctcgc gcgtggccaa gcttgcgaaa gaaacgccgg cgtcgttcgt
     2521 cggttttgac ctgctggctt ccgacggcca gagccggatg gcggcgccgc agagcgagcg
     2581 gcgggcgctc ctcgagcgac tgctcggcga catcggcccg ccggtgtacc tcacgccgat
     2641 gacgcgagac cgcggcacgg caacccggtg gctcgaccag ttcgagggcg ccggtctcga
     2701 cggcgtgatc gcgaagctgg cggacagccc ttatcagccc ggcaaacgcg cgatgatcaa
     2761 ggtaaagcac gcgcgcaccg ccgagtgcgc ggtggccggg ttccgctggc acaagagcgg
     2821 gaaggacgcg gtgggctccc tgctcctcgg cctgtacgat gatcgcggcg tgctccagca
     2881 cgtcggcgtc acctcgtcgt
//
LOCUS       DBNGSB010000029         5764 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_1857862, whole genome shotgun sequence.
ACCESSION   DBNGSB010000029 DBNGSB010000000
VERSION     DBNGSB010000029.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 5764)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5764)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5764
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_1857862"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /locus_tag="AC159B_00835"
     CDS             <1..1074
                     /locus_tag="AC159B_00835"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3376331.1"
                     /translation="KVNWVDVQLQAVEQKLLAAIAGGVPPDVVNLNVEFTVRIAEKNA
                     LLDLDAAVPAEERAKYFEGLWGSARFKGRTYGIPWYIAPPVLMYNTELFKKAGLNPLD
                     PPSSEDEMVDAARQIKDRGKVYGFMPLVDGTRLMHRFLENGLPILSGDGKRAVFNSPA
                     HVAYLQRYLELFKRDYFPEDTLRRGFTGAVERFSAGQLGMLLTGPQFLLRVKASNPEV
                     YSQISVAPYPTGKNKMIHAGLMALAVPRASRHHQEALKFALYVTNDENQLEFSRLAVL
                     LPSTKRAAADPFFKQSTGGPEGKAREIAADELQSSRDLTVVVPNQSELYKVFREAVES
                     AFFGKMSAKQALDWAVREWNARL"
     gene            1056..3275
                     /locus_tag="AC159B_00840"
     CDS             1056..3275
                     /locus_tag="AC159B_00840"
                     /inference="COORDINATES: protein motif:HMM:NF014852.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HIR3376332.1"
                     /translation="MERPAVIQGLVRVVFIVVVLGAVAAAAPTVPDAFLPTPPPIQFF
                     PVPKEVTWGPGVFEISPSTRIVVGDRAADEDLYAARELNEEFRVRFSMTLRVVNAADA
                     GTDRGHIVLGEPSINSLAARMLQAGGITVSATSPGPEGYVLRAVPEGIVVAGSDRRGT
                     FYGVQTLRQLLRPGKSVASVRSVTIRDRPDHPIRAVHVLLDNASAEFHTTLVDRILAP
                     YKFNTLIAEAEHVQWESGRPFWVPDSRGATKAHVRRLVETAREHHIQVIPLIATLGHS
                     EWVFAGLRSDELCRQIAHIPKRLRDEGRTQVTCDRSRAVFPAVYDPERPVMIEGKETT
                     INTALIRPVLQEVADLFRPSFVHIGHDEVRGPSGVSYDLDLYLRDVIALHQLLKDLGV
                     RTMMWGDVLYERRDEVVSEPLWNQVPRDIAVVAWKYEDIRDYPELAYFRQAGFSVLAA
                     TWFRTHNNFHMSRAARIAGALGMVRTTWNGFFQNRAALSRTYQQFYTYLAAATYFWTS
                     GRPQPDTLPSEASLARRFADAWMPKAYRSRPIPGAPVDLRRAVTQRHIDDDGMGWQRK
                     GAEYDLRAVQPGRSRFSGVQFEILDPRGNGKSVVMLKGERDVAEAMPDRVSVAVGRRA
                     GCVAVLHATLDRATNFGDVVGRYEFALGDGTRATQNLLYGRNISSWLADLERGIPSIE
                     QEIAWTGKTRAGNDVNLQMLLWKNPQPDRQVESIDLVAAGGRASPVVFAITTLERCP"
     gene            3286..3864
                     /locus_tag="AC159B_00845"
     CDS             3286..3864
                     /locus_tag="AC159B_00845"
                     /inference="COORDINATES: protein motif:HMM:NF015438.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HIR3376333.1"
                     /translation="MAPTRLPAQQAGGAQRPSRSEAGGILLGAGLNGSVISLHLVRAH
                     GERPQFVAQVGATVGRGLDGDLHGKTRAPGSRRQLLIVDRADLVRLGLQPGDLREQIT
                     VDFPELNALPAGTLLRIGSVTCELTGFCEPCTHIGGLVGVRDPQVFQHTLIGRRGQLA
                     RVTAVEGDGTIRIGDPVTSEGVSTPAVHEQIG"
     gene            3848..4741
                     /locus_tag="AC159B_00850"
     CDS             3848..4741
                     /locus_tag="AC159B_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017869338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3376334.1"
                     /translation="MNKSDSGRRVRTTLIAYLFLAPALGLLAVFTFYPVIFGTILSLF
                     EYDVINPPRYVGLSQFQRLLGDRYFWIAITNSAKYVLVVPVIQLGSILLAVLVNRQLR
                     GIKAFRAAYYIPVITSWPVVGIMWTWMYDQQGIINYVLQTVQAIDRPISWLSHPTLTL
                     YAVMFVTMWKGLGWYMVIYLAGLQSIPTEYEEAAMMDGASRARVFWNVTIPLLRPYIL
                     LASLVSTMAALKVFEEIYVMTRGGPFFSTYTMFMYIFDEAFQELNMGYASALAVVLAA
                     VILVFSAINFRVFRRGGLEYY"
     gene            4741..5547
                     /locus_tag="AC159B_00855"
     CDS             4741..5547
                     /locus_tag="AC159B_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010395548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3376335.1"
                     /translation="MRKVVIYAALVAIAIFLTFPFLWTLIVSLKTKGPIFSIPPDLWP
                     FPLSPANYVGVWETLPLPRYILNSLQITFIGVSLTLLICSLAAYPLARLQFPGKNLVF
                     YAIIATLMLPEHIGLIVNFITLTKLRMVDTFASVYLPSLASIFGIFLLRQAYLSIPQE
                     LEDAARIDGAGDMVIWWRIMLPLITPALATLAIFQFVAFWNSFLWPIIILKTPDKYPL
                     AAGLLYLRGLFAHNTRLIAAGTVIATVPIIVVFLFTQRYFMRGITAGAIR"
     gene            5548..>5764
                     /locus_tag="AC159B_00860"
     CDS             5548..>5764
                     /locus_tag="AC159B_00860"
                     /inference="COORDINATES: protein motif:HMM:NF024941.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4127 family protein"
                     /protein_id="HIR3376336.1"
                     /translation="MSGQILLFVPLDDRPVTMDFVVDLARAAGVEVRTPDRSVLGDRH
                     RAGDVDRIWDWLDGQADVAGATAMIASA"
BASE COUNT         1021 a         1763 c         1818 g         1162 t
ORIGIN      
        1 aaagtcaact gggtcgacgt gcagctccag gcggttgagc agaagctgct ggctgcaatc
       61 gcgggcggcg tccccccgga cgtcgtgaat ctcaacgtcg agttcacggt ccgtatcgcg
      121 gagaagaatg cgctgctcga cctggatgcc gccgtcccgg ctgaagaacg ggcgaaatac
      181 ttcgaggggc tgtgggggtc ggcacggttc aagggtcgca cctacggcat cccatggtat
      241 atcgccccgc ccgtgctgat gtataatacc gagctcttca agaaggccgg gttgaacccg
      301 ctcgacccac cgtcttctga agatgagatg gtcgatgcgg ccaggcagat caaggatcgc
      361 ggcaaggtct acggctttat gccgttggtc gacggcactc ggctgatgca ccggtttctc
      421 gagaacggtc tgccgatcct gagcggcgac ggcaaacgcg cagtgttcaa ctcccccgcg
      481 catgtcgcct atttgcagcg gtaccttgag ctgttcaaac gcgattactt tccggaggat
      541 acgctccggc gtggcttcac cggcgcggtg gaacggttca gcgccggtca gttgggtatg
      601 ctgctgaccg ggccgcagtt tctcttgagg gtcaaggcga gcaacccgga ggtatactcg
      661 caaatttctg ttgcccctta tcctacgggg aagaacaaaa tgattcacgc cggcctgatg
      721 gcgctggcgg tgccgcgcgc cagccggcac catcaggagg ccttgaaatt tgcgctctac
      781 gtcaccaacg acgagaatca gctggagttc agcaggctgg ccgtgctgtt gccgtcgacg
      841 aagcgagccg ccgccgatcc attcttcaag cagagcacag gcgggcctga aggaaaggcg
      901 cgagagatcg cggccgatga attgcagtcc agccgtgacc tgacggtcgt cgttcccaat
      961 cagagcgagc tctacaaggt gttccgcgaa gccgtggaga gcgcattctt cggtaagatg
     1021 tccgcgaagc aggcgcttga ctgggccgtc cgcgagtgga acgcccggct gtaattcagg
     1081 gacttgtgcg agtcgtcttc atcgtagtcg tgctgggcgc agttgccgct gctgcgccga
     1141 ccgtccccga cgcatttctc ccaacacccc caccaataca atttttccct gtgcccaagg
     1201 aagtgacgtg gggccctggg gtattcgaga tttcaccatc aacgcgcatc gtcgttggcg
     1261 accgcgctgc ggatgaagac ctctacgcgg cccgcgaact gaatgaggag tttcgtgtgc
     1321 ggttcagcat gactctgcgc gtagtcaatg ccgccgatgc cggaaccgat cgcgggcata
     1381 tcgttctcgg cgagccatcc atcaattcgc tggcggctag aatgcttcag gccggcggga
     1441 tcacagtctc agcgacatcg ccgggacctg aggggtacgt cctgcgggca gtccccgagg
     1501 gcatcgtggt cgcaggatca gaccgtcgcg gcacgttcta cggcgtgcag accctgcgcc
     1561 aattgctccg gccggggaag tctgtggctt ctgtgcggtc cgtcacgatc cgggaccgtc
     1621 ctgaccatcc catccgcgcc gtgcacgtgc tgctggacaa cgcctccgcc gagtttcaca
     1681 ccacactcgt ggaccggatc ctcgcgccgt acaaattcaa caccctgatc gccgaagccg
     1741 agcatgtgca atgggaaagc ggtagacctt tttgggtccc ggattcgaga ggtgccacca
     1801 aagcgcacgt tcgccggctt gtggagacgg cgcgcgagca ccacatccag gtgattcctc
     1861 tgattgcgac cctggggcac agcgagtggg tgttcgcggg tctgcggagc gacgagctct
     1921 gccgtcagat cgcgcatatt ccgaagcgcc tgcgcgatga aggcaggaca caggtcacgt
     1981 gcgaccgcag ccgggcggtg ttcccggccg tctacgaccc cgagcggcct gtgatgattg
     2041 aggggaagga aaccaccatc aacacagcac tcatccggcc cgtgttgcaa gaggtcgccg
     2101 atctgtttcg accgtcgttt gtgcacattg gacacgacga ggtccgggga ccctcgggcg
     2161 tcagctacga tctcgacctg tacctgcggg acgttatcgc gctgcatcaa ctgctgaaag
     2221 atcttggcgt gcggacgatg atgtggggcg acgtgctcta cgagcgccgt gatgaggtcg
     2281 tctccgagcc gctgtggaat caggtgcccc gcgacatcgc cgtcgtggcg tggaaatatg
     2341 aagacattcg agactatccc gagctggcgt actttcggca ggcgggtttt tcggtgcttg
     2401 cggcgacctg gttccgcacg cacaacaact tccacatgag ccgcgccgcc aggatagccg
     2461 gcgccctggg catggtgcgg acgacctgga atggattttt tcaaaaccgt gctgcgctct
     2521 cgcgcacgta tcaacaattc tacacttacc tcgcggcggc gacgtacttc tggacgagcg
     2581 gacggccgca gcctgacaca ctgccatctg aagcgtcgct ggcgcggcgt tttgccgatg
     2641 catggatgcc caaggcgtat cgctcgcgac cgatcccggg cgcaccggtt gatctgaggc
     2701 gcgccgtcac ccagcgccat atcgatgacg atgggatggg ctggcagcgt aagggtgccg
     2761 agtacgatct gcgggcggtc cagcccggca gatcgcgttt cagcggcgtc cagtttgaaa
     2821 ttctcgaccc cagagggaat gggaagagcg tcgtgatgct gaaaggcgag cgtgacgtgg
     2881 ccgaagcgat gccggaccgc gtctcggtcg cggtggggcg ccgcgccggg tgcgtggctg
     2941 tgcttcatgc tacactcgac cgcgcgacca acttcggaga tgtcgtcggt cgatatgagt
     3001 ttgcgctcgg cgatggcacc cgggcgacgc agaatcttct ctacggccgg aacatcagca
     3061 gctggctcgc agatctggag agaggaatcc cctcgattga acaggaaatc gcgtggacgg
     3121 ggaagacgcg cgcaggcaac gacgtgaatc tgcagatgct gttgtggaag aacccccaac
     3181 ctgaccgaca ggtagagtcc atcgatctcg tggccgccgg cggacgcgca agccccgtcg
     3241 tcttcgcgat caccacgctg gagcgatgcc catgagccaa agaaggtggc gcccacgagg
     3301 ttgcctgcgc agcaggccgg cggcgcacag aggccgagcc gcagcgaggc cgggggaatt
     3361 ttgcttggtg ccgggttgaa cggaagcgta atcagcctgc acctggtgcg ggcgcacggt
     3421 gagcgtccgc agttcgttgc tcaagtcggg gcgacggtcg gacgcgggct cgatggagat
     3481 ctgcacggga agactcgagc gcccggcagc cgcaggcagc tcctgatcgt cgaccgcgcg
     3541 gatctcgtgc gcctgggatt gcagcccggg gatctgcggg agcagattac ggtggatttt
     3601 cccgaactca acgcgctgcc ggcgggaacg ctgttgcgca tcggctccgt cacctgtgag
     3661 ctcacgggtt tctgtgaacc ctgcacgcac atcggcggcc tggtcggcgt ccgtgatccg
     3721 caggtatttc agcacaccct gataggccgt cgaggccagc tcgcccgtgt gacggccgtc
     3781 gagggcgacg gaacgatcag gatcggtgat ccggtaacct ccgaaggggt gtcgacgccc
     3841 gcagtgcatg aacaaatcgg atagcggccg gcgcgtccgg acgacgctta tcgcctacct
     3901 ctttctcgcg cccgcgctcg gactacttgc ggtcttcacg ttctacccgg tcattttcgg
     3961 aacgatcctc agcctgttcg aatacgatgt catcaatccg ccccggtacg tcggcctgag
     4021 tcaattccag cgcctgctgg gcgaccgcta cttctggatc gcgatcacga actcggccaa
     4081 gtatgtgctc gtcgtgccgg tgattcagct cggctccatc ctcctcgccg tgctcgtcaa
     4141 ccggcagctc cgcggcatca aggccttccg cgccgcgtac tacatcccgg tgatcacctc
     4201 atggccggtc gtgggcatca tgtggacctg gatgtacgat cagcagggga tcatcaacta
     4261 tgttctgcag accgtgcagg cgatcgaccg gcccatctcg tggctcagcc atccgacgct
     4321 caccctgtac gccgtcatgt ttgtgacgat gtggaaggga ctcggatggt acatggtcat
     4381 ttacctggcg ggcctgcagt cgattcccac tgagtatgaa gaagccgcga tgatggacgg
     4441 tgcgtcccgc gcgcgcgtct tctggaatgt cacgattccg ctgctgcgcc cgtacattct
     4501 gctcgcctcg ctggttagca ccatggcggc gctgaaagtc tttgaggaga tctatgtgat
     4561 gacgcgcggc ggcccgttct tctcgaccta cacgatgttc atgtacatct tcgatgaagc
     4621 gttccaggag ctgaacatgg gatacgcgtc ggcgctcgcg gtggtcctgg ccgcagtcat
     4681 tctcgtgttc tcggccatca acttccgcgt cttccggcgc gggggactgg aatactactg
     4741 atgcgcaaag tggtgatcta cgccgccctc gtcgccatcg cgatcttcct aacgttccca
     4801 tttctgtgga cgctgatcgt gtcgctcaaa accaaaggcc cgatcttctc gatcccgccc
     4861 gacctctggc cctttccgct gtcgccggcc aactacgtgg gagtctggga gacgctgccg
     4921 ctgcctcgat atatcctgaa cagcctgcag atcaccttta tcggcgtgtc gttgacgctg
     4981 ttgatttgtt cgctggccgc ctacccgctg gcacgcctcc agtttccggg gaagaacctg
     5041 gtgttctacg ccatcatcgc gacgctcatg ctgcccgagc acatcggcct gatcgtaaac
     5101 ttcatcacgc tgacgaagct gcgaatggtc gatacgttcg catcggtcta ccttcccagc
     5161 ctggcgagca tcttcggcat tttcttgctg cgccaggcgt acctctcgat ccctcaggaa
     5221 ctggaggacg ccgctcggat cgacggcgcc ggcgatatgg tgatctggtg gaggatcatg
     5281 ctgccgctca ttacaccggc cttggcgacg ctcgcgatct tccagtttgt ggcgttctgg
     5341 aacagctttc tgtggccgat catcatcctg aaaacccctg acaagtatcc gctcgcggcc
     5401 ggtctcttgt atctgcgggg attgttcgcg cataacacgc gtctcatcgc ggccggcacg
     5461 gtgatcgcga cggtgccgat catcgtcgtg ttcttgttta ctcagcgtta cttcatgcgg
     5521 gggatcaccg cgggagccat tcgctagatg agcggtcaga ttttgttgtt cgttccgctc
     5581 gacgacaggc ccgtgacgat ggacttcgtg gtcgacctcg cgcgcgcggc cggagtggag
     5641 gtccgcacac ccgatcggtc ggtgcttggc gaccggcacc gcgccggcga cgtcgatcgc
     5701 atctgggact ggctcgacgg tcaggcagac gtggctgggg cgacggcgat gattgcgtcg
     5761 gctg
//
LOCUS       DBNGSB010000030         4873 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_1859522, whole genome shotgun sequence.
ACCESSION   DBNGSB010000030 DBNGSB010000000
VERSION     DBNGSB010000030.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 4873)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4873)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4873
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_1859522"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..1575)
                     /locus_tag="AC159B_00865"
     CDS             complement(226..1575)
                     /locus_tag="AC159B_00865"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF015531.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR3376337.1"
                     /translation="MKLIRTHRWREILGTLIMVAVLSPLAIGLPALAADQSLVLEALG
                     VLQQHYVDRIDPVKMLNAALAGVRAQLPAAGTMATLSDIPADLSEDEAKRIFLDRFTT
                     AVNASNGNSTQTQLAHAAIRGMTDSFKDSHTGFLTPQQNAERRQRQRGQAGFSGVGII
                     LAPRDGKFFVMTTIPGGPAEAAGVKEFDRILRVNDVSTSGMQVGDVSGMVRGPSGTQV
                     SLTLQRPGLTDPMVVTVTRAPISTPAILKSEILEGGVGYIKLWQFVERTGRDFRGVVS
                     QMQGQGMRALVLDLRGNSGGFLTELNSVLNAIMPAGLPVYTEIRKGGNTRIFRTVGPA
                     LLPPSLPMVVLIDENSASAAELLAMAIKEHRRGQLIGEKTAGAVEASLLLDLSDGSAL
                     SVTTFRLASGMGVRLEGAGVDPDMPTALTTADIEAGQDKPLGTAVRLVRQYLALPAR"
     gene            complement(1587..2312)
                     /locus_tag="AC159B_00870"
     CDS             complement(1587..2312)
                     /locus_tag="AC159B_00870"
                     /inference="COORDINATES: protein motif:HMM:NF014713.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HIR3376338.1"
                     /translation="MPEVNLILAFAAGMLGFASPCIVPLIPGYVSFVSGLSLSEMDPD
                     ERRRHLGRVWLSTLIFVLGFSVIFTAMGASATVAGNFILSNRQWLSRIGGAVVIVLGL
                     SVLGVLKIPALYRERRVHVRPVGGLWGALPVGMAFAFAWTPCVGPVLAAILTLAATAV
                     RASDGALLLFSYSLGLGVPFLVAAGLLSTAFDALSWFRRHGRAIEAVSGAFLVVMGAA
                     LMFDLVFRLNTWILRLVPFQPTI"
     gene            complement(2457..3332)
                     /locus_tag="AC159B_00875"
     CDS             complement(2457..3332)
                     /locus_tag="AC159B_00875"
                     /EC_number="3.2.2.-"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013328.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fpg/Nei family DNA glycosylase"
                     /protein_id="HIR3376339.1"
                     /translation="MPELPFIQVLVENLAARITGRTVQHVHLASPSVLKTVDPPLSEA
                     QGRSIETVRRTGKLILLDLTDGLSIAFHLMRNGRLQVGPARVSGQRSPRPSKDVAVIM
                     TLDDHQELRFVEIGPKKRAALYVLPTEGILKREPLAGLGIDPLSDEFTPDRLKEMLAL
                     DSGQLKHFLTLQQYITGIGNAFSDEILWEAKLSPFVRASKLRPEETATLYRGIRATLM
                     RGLEEHRRVLMDNLPTKEPVQLLHVHRHGGEPCPRCGTKIAEVAYAERETYYCPSCQT
                     AGKVYADRRMSRLLK"
     gene            complement(3398..3964)
                     /locus_tag="AC159B_00880"
     CDS             complement(3398..3964)
                     /locus_tag="AC159B_00880"
                     /inference="COORDINATES: protein motif:HMM:NF014041.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF84 family protein"
                     /protein_id="HIR3376340.1"
                     /translation="MKVAIGSTSQSKLRAARAVFARVDAKAEIQAVTVESSVSDQPVS
                     DGEAIAGALHRARESRALADADLGVGIEGGVHADTHGVWMCAWVAIIDRQGREGLGCG
                     MRLRLPDWIASRALKREPVGEIVDRFLGQQGAHEALGVIGLLTRRLVDRQAALEQALL
                     AALAPFLSEEMYAREPESSGRKQEQGEQ"
     gene            4025..4798
                     /gene="lgt"
                     /locus_tag="AC159B_00885"
     CDS             4025..4798
                     /gene="lgt"
                     /locus_tag="AC159B_00885"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR3376341.1"
                     /translation="MNPVIVEIGPLVIRWYGVMMALMIVLAVATAYRYGPRFGVPARE
                     LDRLTLPFVLTAVIGARVGYVLSHPAEFTNPLEILRIDHGGLTSHGAIAGALVLLWMT
                     SHRRHLSFWSLADTTVWAVPLGNILVRFGNFMNGELYGDVTGLPWGVTFPNAPGPRHP
                     LQIYEMILAAGVLLAAARVAQRRRFDGEVFWTTMVLSSIARIALDLLRSEDRFFWVLT
                     LGHIPALILLFVGIGFLVQRASTQSPPSTPSSPSQAKIS"
BASE COUNT          933 a         1569 c         1435 g          936 t
ORIGIN      
        1 ttcgaactct ccagacgcac tgcgcttgcc agcaggccaa caatggccgc gcggctgcga
       61 tcaatctggt cgaggagagc ctggtcggca gtagtcaaag gccttagggc acagggctta
      121 gggcttagaa atgaaacggg aagagagaca ttcctctctt cccgttatcc cagcgaactt
      181 cgttttcctt tactgagctc tacgagctgg ccctaaaccc tgaagttacc gtgccggcag
      241 cgcaagatac tggcgcacga ggcgaactgc cgtacccaac ggcttgtcct gacccgcttc
      301 gatgtccgct gtggtgaggg cggtcggcat gtcggggtcg acgcctgcgc cttcgaggcg
      361 cacgcccatc ccggacgcga gccggaatgt cgtgacactg agcgccgaac cgtccgagag
      421 gtcgagcaac aggcttgctt ccaccgctcc ggccgtcttt tctccgatca gctgaccgcg
      481 gcgatgttcc ttgatggcca tcgccagcaa ctctgccgcg gaggcactgt tctcatcgat
      541 caacaccacc atcggcaggg acggcggcag cagcgccggc cccaccgtgc gaaagatgcg
      601 cgtgttgccc cctttgcgga tttccgtgta cacggggagg cctgccggca tgatcgcatt
      661 cagtacgctg ttgagttctg tcagaaatcc gccgctgttc cctcgaaggt cgagtaccag
      721 cgcgcgcatg ccctgcccct gcatctggga gacgactcca cggaagtctc gaccggtccg
      781 ttccacgaac tgccagagct tgatgtatcc tacgccgcct tcaagaatct cgctcttcaa
      841 aatggccggg gtcgagatcg gcgcgcgcgt cacggtcacc accattggat ctgtgagacc
      901 cggccgctgc agcgtgagcg acacctgggt gcccgacggg cctcggacca tacccgacac
      961 gtcacccacc tgcatacctg aggtcgagac atcgttgact ctcaggattc gatcgaattc
     1021 cttgacgccg gcagcctcgg cgggcccacc cggaatcgtt gtcatgacaa aaaacttgcc
     1081 gtcacgcggg gcgaggatga tgccgacccc cgaaaatcct gcctgtcccc gctgtcgctg
     1141 acgccgctcc gcattttgct gcggggtcaa gaagcctgtg tgcgaatctt tgaaactatc
     1201 cgtcattccg cggatcgccg catgagccag ttgagtttgt gtgctattgc cattgctggc
     1261 gttcacggca gtcgtaaatc ggtcgaggaa tatccgcttg gcctcgtcct cggagagatc
     1321 cgccggaata tcactcaacg tcgccatcgt tcccgccgcc ggcaattgcg cccgaactcc
     1381 cgccagggcg gcgttgagca tcttcaccgg gtcgatccga tcgacatagt gctgctgcaa
     1441 cactcccaaa gcttccaata ccagagattg gtcggccgcc agggcaggca gcccgatcgc
     1501 cagaggcgac agcaccgcga ccatgatgag cgtgccgagt atttctctcc agcggtgagt
     1561 ccggataagt ttcatgccct ctccctctat attgtgggct gaaatggaac cagccgcagg
     1621 atccaggtgt tgagtctaaa caccaggtcg aacatcaatg cggcccccat gacaaccaag
     1681 aacgctccgc tcaccgcttc gatcgccctg ccgtgccgcc tgaaccaaga gagggcgtca
     1741 aaagccgtag agagcaaccc cgctgccaca agaaacggga ccccgagccc caatgaatac
     1801 gaaaacagca acagcgctcc atcgcttgcg cgcacggccg tcgcggccag tgtcaagatg
     1861 gccgcgagca ccgggccgac gcagggcgtc caagcaaacg caaatgccat cccgacgggt
     1921 agcgcgcccc acagtcctcc gacggggcgc acgtgaaccc gccgttcacg gtagagcgcg
     1981 ggaatcttca acacgccgag cacggacagc ccaagcacga tcacgacagc gccgccgatg
     2041 cggctcagcc actgccggtt gctcaggatg aagttgccgg cgacagtggc cgaagcgccc
     2101 atggcggtga agatcactga gaatccgagc acaaagatca gtgttgacag ccacacccgg
     2161 ccaagatgcc ggcggcgctc atcaggatcc atctcactga gagacagacc cgagacgaac
     2221 gacacgtagc ccggtatgag cggcacaatg cacggagagg caaacccgag catgccggca
     2281 gcgaaagcga gaatcagatt gacttcaggc atttagctct cgaactccgg gtctggaaat
     2341 tagagacgca ccgcagcatc cctgccagtg acttcatgcc cgaaacgcac gcgtcctaga
     2401 atacggctct accactaata cggtcaacat gcgccggggg tttacttgcc tattctctac
     2461 ttgagtagcc gggacatgcg ccggtcggcg tagaccttgc ccgcggtctg gcaggatggg
     2521 caataatacg tctctctttc cgcgtaggcc acctcggcaa tctttgtccc gcaacgtggg
     2581 cacggctcgc cgccgtgccg gtgcacatgg agcaactgca cgggctcttt ggtcggcaga
     2641 ttgtccataa gcaccctgcg gtgctcttcg agtccccgca tgagtgtcgc gcgaatcccc
     2701 cgatagagcg ttgccgtttc ctcgggcctg agcttcgagg cccgtacaaa cggggacagt
     2761 ttcgcctccc agaggatttc gtccgaaaac gcgttcccga tcccggtgat gtattgttgt
     2821 agggtcagga aatgtttgag ttgccccgaa tccagcgcga gcatctcttt cagccgatcc
     2881 ggggtgaatt catcactgag cggatcgata ccgagccccg cgagcggttc ccgtttcagg
     2941 atcccctcgg tcggcaggac gtacagcgcc gcgcgcttct tcggaccgat ttcgacgaat
     3001 cgcaactcct ggtgatcgtc gagcgtcatg atgaccgcga cgtccttgga tggacgcggc
     3061 gatcgctggc cgctcacccg ggcaggcccg acttgcagcc gaccattccg catcaaatga
     3121 aatgcgatgg agaggccgtc ggtgagatca aggaggatga gtttgccggt gcggcgcact
     3181 gtctcaatcg agcgcccctg cgcctcgctg agcggcgggt cgactgtttt cagaactgag
     3241 ggactggcga gatgaacgtg ctgaaccgtg cgccccgtga ttcgggccgc gaggttctcg
     3301 acgagcacct gaatgaatgg cagctcaggc atgtcacagg cctacggagg gggaaaaagg
     3361 ttcatggaag gaggtgacct tcatgattct cctgttgcta ttgctcgccc tgttcctgct
     3421 tgcggccaga tgattcgggc tctcgagcgt acatctcctc ggagaggaac ggcgccagtg
     3481 cggcgaggag cgcctgctcc aatgccgcct gccggtctac caaccggcgt gtaagcaggc
     3541 caatcacccc gagcgcctcg tgcgctcctt gctgtcccaa gaacctgtcg acgatctcac
     3601 cgactggctc gcgtttgagc gcgcggctcg cgatccagtc gggaaggcgc aaccgcatgc
     3661 cgcagcccag accctcgcgg ccctgccggt cgatgatcgc cacccaggcg cacatccaca
     3721 cgccgtgagt gtccgcatga acgcccccct cgatgcccac gccgagatcg gcgtcggcga
     3781 gagcccggct ttcgcgggcg cgatgaagcg cgcccgcgat cgcctcgccg tcgctgaccg
     3841 gctgatcgga caccgaggat tccacggtca cggcttgaat ctccgccttc gcgtccacgc
     3901 gagcgaagac ggcccgcgct gcgcgtagct tcgactggct tgtcgacccg atcgcgacct
     3961 tcatcggcgt ctccgttccc cgttccccgt cagccgttcg cggtgtattg tgttgagtgc
     4021 gtcaatgaac cccgtcatcg tcgagattgg acctctcgtc atccgttggt acggcgtgat
     4081 gatggctctc atgatcgtgc tggcggtcgc caccgcgtac cgatatgggc cgcgcttcgg
     4141 cgttcccgca cgggagctcg accggttgac gctcccgttc gtgctgaccg cagtcatcgg
     4201 cgcccgcgtg ggctacgtgc tctctcatcc ggccgagttc acgaatcctc tcgagatcct
     4261 gcgcattgat catggaggat taacatcgca cggcgccatc gccggagcac tcgtattgct
     4321 gtggatgacg agccaccgtc gccatctgtc gttctggagc ctcgcggata ccacggtgtg
     4381 ggcggtcccc ctgggaaaca tcctcgtccg ctttggaaat ttcatgaacg gcgagctcta
     4441 cggagatgtg acgggccttc catggggggt gacattcccc aacgcgcctg gcccgcgtca
     4501 cccgctgcag atctacgaga tgatcctggc ggcaggcgtg ctcctcgccg cagcgcgggt
     4561 tgcccagcgg cggcggttcg acggcgaagt tttctggacg acgatggtcc tgagttcaat
     4621 cgcgcggatc gcgctcgacc tgctgcgaag cgaagatcgc ttcttttggg ttctcacgtt
     4681 aggacacatt cctgcattga ttctgctctt cgtcggaatc gggttcttag tccaaagagc
     4741 gtctacccag tctcccccgt ctaccccgtc ttccccgtct caagcaaaaa tatcttgatc
     4801 tcttgagcat gtagacaggg gagactggga agacagggta gacggggaag atgcagtgcg
     4861 tcagaactca tcc
//
LOCUS       DBNGSB010000031         6448 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_326105, whole genome shotgun sequence.
ACCESSION   DBNGSB010000031 DBNGSB010000000
VERSION     DBNGSB010000031.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 6448)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6448)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6448
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_326105"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..645
                     /locus_tag="AC159B_00890"
     CDS             115..645
                     /locus_tag="AC159B_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR3376342.1"
                     /translation="MERLRKSTRVYDGRILSLRVDEVELSSGRVTTREIVEHRGAVAI
                     VPLTDAGRILLVRQYRTAAGRALLEIPAGTIEPNEDVLVCLQRELAEEVGMQADRVQH
                     LVTFLPSPGFLTEQVHLYLATGLSPATLEREEEDLEVVPMPLAQAQSLIGTEIIDAKS
                     IIGLLMALSPHHHAGV"
     gene            642..1601
                     /gene="xerD"
                     /locus_tag="AC159B_00895"
     CDS             642..1601
                     /gene="xerD"
                     /locus_tag="AC159B_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HIR3376343.1"
                     /translation="MRQAGPPYGAHIEAFLAYALAEAGLARASADSYRHDLVEFAEFM
                     ASRGISKPSEVDRAALTVYLLTLRRHGRAAATIKRRAAAIRAFYRFLLREEELEHDPT
                     LDLAPPKLPARLPRVLTIDEVDRLLAAPDPLSPRGNRDRAMLELMYASGLRVSELVNL
                     NLGDADLAHEVVRTIGKGDRERLVPIGTKAVQALRSYLLHARPALSKTRGTQALFVNR
                     RGGRLTRQACWKFVRAYARQARIAKPLTPHMLRHSFATHLLEGGADLRAVQEMLGHAS
                     INTTQVYTHVTRDRLREVYRQAHPRDGMRIATRPKTKTVGRKQ"
     gene            1626..2810
                     /locus_tag="AC159B_00900"
     CDS             1626..2810
                     /locus_tag="AC159B_00900"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - metabolic compound salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HIR3376344.1"
                     /translation="MFTRAIIIVLDSAGVGELPDADRYGDVGSSTIPHTAEAVGGLHL
                     PTLESLGLGRIVPIRGVAPVANPAAAFGKLTERSPGKDSTTGHWELMGIILDRPFPTY
                     PNGFPRALIEAFEREIGTKVLGNYAASGTEIIQQLGPEHLRSKHPIVYTSADSVFQIA
                     AHEDVVPIPRLYEMCEIARRILTGEHSVSRVIARPFVGTLGAFKRTDRRRDFSLPPPT
                     PTVLDHAVHSGLPVVGVGKVPDLFAGRGITRSVHTHDDLYGVTQTAAAMDETRQGIIF
                     ANLVDLDSKYGHRNDPRGYAADLAAIDAKLPAILQKLRPEDLLMFTADHGNDPTTPST
                     DHSREYVPLLVAGPNVRGGVDLGTRRTFADVGATVAEALGVTWTGAGESVLSSLLGVR
                     TR"
     gene            2807..3631
                     /locus_tag="AC159B_00905"
     CDS             2807..3631
                     /locus_tag="AC159B_00905"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIR3376345.1"
                     /translation="MTAREQINQASSFVKQRTKIKPKIALILGSGLGALAHEIEADAT
                     IPYSEIPNFPASTVEGHTGQLVLGRLEGKAVVAMQGRVHFYEGYTLQHIVFPIRVMHA
                     LGAGALVVSNAAGGINRQWHRGDLMIIADHINFFGTNPLIGANDPDLGPRFPDMSQPY
                     DPEFIALAERSAGADGLVIRKGVYVGVSGPSYETLAELRMLRGWGADAVGMSTVPEVI
                     AARHLGMRVLGITAITDMATGEQVQQVTHEEVIAVAKEVEPKFIRLVKRILREMQV"
     gene            3749..5053
                     /locus_tag="AC159B_00910"
     CDS             3749..5053
                     /locus_tag="AC159B_00910"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019006503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HIR3376346.1"
                     /translation="MRAYEIIQKKRDGGELSAEEIEGFVQGFTSGSVPDYQVSAFLMA
                     VYFRGMTAEETTALTLAMVRSGETLDLSAIRGTKVDKHSTGGVGDKTSLVLIPLVAAA
                     GAPVAKLSGRGLGHTGGTLDKLESIPGFTTELSAEAFVSQVNTIGCAIAGQTADLVPA
                     DKKLYALRDVTATVDSIPLIAASVMSKKIAAGSDGIVLDVKTGSGAFMKSLDGSRELA
                     KTMVAIGNEAGRRTVAVISDMDQPLGYAVGNALEVREAIETLAGEGPDDLRELCVALG
                     AQMLVLAGITRSLEDAGAILERLLSDGRALDKFKEMTRAQRGDPACVDDPERLPQAPI
                     LLPVPAPSAGVVTGIDAERIGLAAMRLGAGRAKKEDTIDPAVGIVLKKKVRDQVSKGE
                     ALAQIQAAHKDWAEQALALIQAAYSIGRTPPRTRPLIHEIVT"
     gene            5054..5617
                     /locus_tag="AC159B_00915"
     CDS             5054..5617
                     /locus_tag="AC159B_00915"
                     /inference="COORDINATES: protein motif:HMM:NF018154.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3376347.1"
                     /translation="MRIPIPDHSLVMLCGPAGSGKSTFARKHFPETAIVSSDRCRAMI
                     GDDESNLGVSRDAFDLFHQIIAARLRNRRLAVADSTALGADARRELRQLARRAGVPVI
                     LIVFDVSEATSAKQDETRPRRVGRPVIREHWERLQEALKQISRESYDQVVILSEEDVR
                     RATVEIGRRRGAEPASRGTEQGGRDGD"
     gene            5607..>6448
                     /locus_tag="AC159B_00920"
     CDS             5607..>6448
                     /locus_tag="AC159B_00920"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - (3S)-3-hydroxyacyl-CoA
                     dehydrogenase (NAD+) activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3376348.1"
                     /translation="MEIKRVGVVGCGLMGSGIAEVCALGGYQVAVSEVDDDLLKRGLS
                     RLEASMQRGVQRGKLAEADAAAARKRIRGTTNLSDLASSDLIIEAVVEKMDLKKEVYR
                     ALDEACPPETIFASNTSSLSITEMASVTKRRERFLGMHFFNPVPIMKLVELIRGLQTS
                     EETMDTGRRFATAVGKTVVVCKDSPGFIVNLLLIPYLLDSVRALELGIATKEDIDTAI
                     VLGLAHPMGPLTLLDYVGIDTTYFIAEAMFQEFKDTRYAAPPLMKQMVLAGLHGRKTG
                     RGFY"
BASE COUNT         1249 a         1969 c         2110 g         1120 t
ORIGIN      
        1 gcacctgatc caatctatac aatcttcaca atcacactat ctacacaatt aggagagtgg
       61 attgagcaat tgtgcggatt gtgcagattg tgaagatcgt gcagattgaa ggtcgtggag
      121 cggctccgga aatcgacgcg ggtctacgac ggacgcatcc tctcgctacg cgtcgatgag
      181 gttgagcttt ccagcggccg cgtgactact cgggaaatcg tggagcatcg cggcgcggtg
      241 gccatcgtgc cactgacgga cgccggccgc atcctcctcg tccggcagta ccgcaccgcc
      301 gccgggcgcg cgcttctcga gattcccgcc ggaaccattg agccaaacga ggatgtgttg
      361 gtgtgcctgc agcgtgaact cgcggaggaa gtgggaatgc aggccgatcg agtgcagcac
      421 ctcgtgacgt tcctgccctc tccggggttt ctcaccgagc aggtgcatct ctacctggcc
      481 acaggtctct cgccggcaac gctcgagcgg gaagaggaag acctggaggt tgtgccgatg
      541 ccgctggcgc aggcgcagtc gttgatcggc acagagatca tcgacgcgaa gtcgatcatc
      601 ggactgctca tggccctctc tccgcaccac cacgccggcg tatgaggcag gccggtcctc
      661 cctacggcgc ccacattgag gcgtttctgg cttacgcgct ggccgaagcg ggtctggccc
      721 gcgcaagcgc ggattcgtac cgccacgatc ttgtcgagtt cgcggagttc atggcgtctc
      781 gcgggatcag caaaccctcg gaggtcgatc gcgcggccct caccgtatac ctgctcacgc
      841 tgcggcggca tggccgcgct gcggcgacga tcaagcgccg ggcggcggca attcgggcgt
      901 tctaccgctt cctcttgcgt gaggaggaat tggaacacga cccaacactc gacctggcgc
      961 ctccgaaact cccggcaagg ttaccgaggg tcctcacgat cgacgaggtc gaccggctgc
     1021 tcgccgcacc ggatccgctg tcgccgcgag ggaatcgtga ccgcgcgatg cttgagctga
     1081 tgtacgccag cggactgcgc gtctccgaac ttgtcaatct gaatctgggc gatgcggacc
     1141 tggcccatga ggtcgtacgg acgatcggta agggcgacag ggaacgcctc gttcccatcg
     1201 ggacaaaggc cgtccaggcc ctccggtcct atctcctgca tgcgcgtcct gcgctctcaa
     1261 aaacgcgcgg aacgcaggcg ctgttcgtaa accgtcgcgg cggcagactc acaaggcagg
     1321 cctgctggaa attcgtgcga gcgtacgccc ggcaggccag aatcgcgaag ccgctgacgc
     1381 cgcacatgct caggcactcg tttgcgacgc atctgctcga gggaggagcc gacctgcgcg
     1441 cggttcagga aatgctgggg catgcgtcga tcaacacgac ccaggtttat actcacgtca
     1501 cccgcgatcg attgcgcgag gtctaccggc aggcccatcc tcgcgacgga atgcgcatcg
     1561 ccacccgccc aaagacgaag acagtaggca gaaagcagta ggccacgaac tcccgaaggg
     1621 tgagcgtgtt tacgcgggcc atcatcatcg tgctcgacag cgccggcgtc ggcgaactgc
     1681 ctgatgcgga tcgctacggg gacgtaggca gcagcacaat cccgcatacg gcggaggccg
     1741 tgggtgggct gcacctgccc acgctggagt cgttgggtct cggccggatt gtgccgattc
     1801 gaggagtggc gcctgtcgcc aatcccgccg ccgcattcgg caagctgaca gaacgctcac
     1861 ccggcaaaga tagcaccacc ggacactggg agctcatggg gatcatcctg gaccgtccct
     1921 tcccaaccta tcccaacggt ttcccgcgcg cactcatcga agcgttcgag cgcgagatcg
     1981 gcacgaaggt cctcggcaac tacgcggcat ccggcaccga gatcattcaa cagctcggac
     2041 cggagcatct gaggagcaag caccctatcg tgtacacctc ggccgacagc gtgttccaga
     2101 tcgcggccca cgaggatgta gttccaatcc cccgcctcta tgagatgtgc gagatcgcgc
     2161 ggcggatctt gaccggagag cactctgtga gccgggtgat cgccaggccg ttcgtgggga
     2221 cgctcggcgc gttcaagcga accgaccgtc gccgcgactt ctcgctcccg cctccgaccc
     2281 ctacggtgct cgatcacgcg gtccactctg gcctccctgt ggtgggagtg gggaaggttc
     2341 cggacttatt tgcggggcgc gggatcaccc gaagcgtcca tacccacgac gatctttacg
     2401 gcgtcacgca gaccgcggcg gccatggacg agacgcgcca gggcatcatc ttcgcaaacc
     2461 ttgtggacct ggactccaaa tatggccacc gcaacgatcc gcgcgggtac gcggcagatc
     2521 ttgcagcgat cgatgcgaag ctcccggcga tcctgcaaaa gctgaggccg gaggatctcc
     2581 tgatgttcac ggccgaccac ggcaacgatc cgaccacgcc gagcacggat cactccagag
     2641 aatatgtgcc gctccttgtc gcggggccga acgtgcgggg cggagtcgac cttggaaccc
     2701 gccgcacgtt tgcggatgtg ggtgcgacgg tcgccgaagc cctcggagtg acgtggacgg
     2761 gagccggcga aagtgtgctt tcctcactgc tcggggtgcg aacgagatga cagcgcggga
     2821 gcagatcaat caggccagct cgtttgtcaa gcagcgcaca aagatcaaac caaagatcgc
     2881 gttgatcctg gggtccggtc tgggcgcgct ggctcatgag attgaagctg acgccacgat
     2941 tccgtattcg gagatcccca atttcccggc ttcaacggtg gaaggccata ccggtcagct
     3001 tgtccttggc aggctggaag gcaaggccgt cgtggcgatg cagggccgcg tgcacttcta
     3061 cgagggatac acgctgcagc acatcgtctt cccgatccgg gtgatgcacg ccctgggtgc
     3121 aggggcgctg gtcgtcagca atgcggcggg cgggatcaac cggcagtggc accgaggcga
     3181 tctcatgatt attgccgacc acatcaactt cttcggcacg aacccgctga tcggcgcgaa
     3241 cgatcctgac ctcggccctc gtttcccgga catgtcgcag ccgtacgatc cggagttcat
     3301 cgccctggcc gaacgttcag ctggggccga cgggctggtc atccgcaagg gcgtgtatgt
     3361 tggcgtgtcc ggcccgagct acgagaccct agccgagctg cggatgctca gaggctgggg
     3421 cgccgacgct gtggggatgt ccaccgtgcc cgaagtgatt gccgcgcggc acctgggcat
     3481 gcgggtgctc ggaattaccg cgatcaccga catggcgact ggcgaacagg tgcagcaggt
     3541 cacccacgaa gaggtgatcg cagtcgcgaa agaggtggag ccgaagttca tccgccttgt
     3601 gaaacgcatt ctaagggaga tgcaagtgtg agtgggttca acgctggagg cggggaggcc
     3661 agaccctctc cccgactgca actgatacgg ggacccctca gggtccggcc cccccgcctc
     3721 cagcatcgcc atcacaagta atccctccat gcgggcttac gaaatcattc agaagaagcg
     3781 cgacggcggt gaactttccg ctgaggaaat tgaggggttt gttcaggggt ttacctccgg
     3841 gagtgtcccc gactatcagg taagcgcatt cctcatggcg gtgtacttcc gcggcatgac
     3901 agcggaggaa acgaccgcat tgacattggc gatggtgcgg tcgggtgaaa ccctcgatct
     3961 gagcgcaatt cgtggcacga aggtggataa gcacagtacg ggcggggtag gggacaagac
     4021 atcgctcgtg ctcatcccgc tcgtggccgc ggcaggagcg ccggtcgcga aactttccgg
     4081 ccggggactc gggcataccg gggggaccct cgataagctc gagtcgattc caggctttac
     4141 gactgagctc tccgcggagg cgtttgtcag ccaggtcaac acgattggct gcgccatcgc
     4201 cggacagacc gccgatctcg tgcccgccga caaaaaactg tatgcgctgc gcgacgtgac
     4261 cgccaccgtg gacagcattc ccttgatcgc cgccagcgta atgagcaaga agatcgcggc
     4321 aggcagcgac gggatcgtgc tggacgtgaa gaccgggagc ggcgcgttca tgaaatcgtt
     4381 ggacggatcc cgcgaactcg cgaagaccat ggtggccatt ggtaacgagg ccgggcgcag
     4441 gacggttgca gtgatcagcg acatggatca accgctgggc tacgctgtcg gcaatgccct
     4501 ggaggtgcgc gaggcgatcg agacgctcgc gggcgagggg ccggatgacc tgcgggagct
     4561 ctgcgtcgcg ctcggcgcgc agatgctggt cctcgcgggg atcacgaggt ctctcgagga
     4621 tgccggggcc attctcgagc ggttgctttc cgacggccgg gcgctcgaca agttcaagga
     4681 gatgacacgc gcgcagcgtg gggacccagc ctgtgttgat gatcccgagc gtcttcccca
     4741 ggcgcctatc ttacttccgg tacctgcccc ctccgcagga gtcgtgactg gaattgacgc
     4801 cgaacgcatc gggctcgccg cgatgaggtt gggcgcgggc cgggcgaaaa aggaggacac
     4861 gattgatccg gccgtcggga tcgtgctcaa aaagaaagtc cgcgatcagg tgagcaaggg
     4921 agaggccctc gcgcagatcc aggccgcgca caaggattgg gcggagcagg cactcgcgct
     4981 gatacaggca gcgtattcca tcgggcgtac gccgccgcga acgcggccgc tgattcacga
     5041 gatcgtgacc tagatgcgca taccaattcc tgatcactca ttggtgatgc tctgcggacc
     5101 cgcgggcagc ggcaagagta cgttcgcgcg gaagcacttt cccgaaacgg ccatcgtctc
     5161 atccgaccgg tgccgggcaa tgatcggcga tgatgagtcg aacctcggag tgtcgcgtga
     5221 tgcgttcgac ctcttccacc agatcatcgc tgcccgactg cgaaaccgca ggctggccgt
     5281 cgccgacagc accgcgctcg gcgccgacgc gcgccgcgag ttgaggcaac tggcgcgccg
     5341 ggcgggtgtt ccggtcatcc tgatcgtgtt cgatgtcagc gaagcgacca gcgcgaagca
     5401 ggacgagacc cgtccacggc gggttggacg cccggtgata cgcgagcact gggagcgcct
     5461 tcaggaagcg ctcaaacaga tttcgcgcga atcgtatgat caggtcgtca tcctcagcga
     5521 ggaggatgtt cggcgggcga ccgtcgagat cgggaggcga cgcggcgcgg aaccggcatc
     5581 ccgaggcaca gaacaggggg gaagagatgg agattaagcg cgtgggtgtc gttggctgcg
     5641 gcctcatggg atccgggatt gcggaagtct gcgcgctggg cggatatcag gtggcggtca
     5701 gcgaggtgga cgacgatttg ctcaagagag gcctctcccg cctggaggcc tcgatgcagc
     5761 ggggagtgca gcgagggaaa ctggccgagg ccgacgcggc tgccgcgcgc aagaggatcc
     5821 gcgggacgac gaacctgagt gatctcgcct cgtccgatct catcatcgaa gctgtggtcg
     5881 aaaagatgga cttgaagaag gaagtgtatc gcgcgctcga cgaggcctgt ccgccggaga
     5941 cgattttcgc gagcaacacg tcgtcgctat cgatcacgga aatggcgtcc gtgacgaaac
     6001 ggcgcgagcg tttcctcggg atgcacttct tcaatccagt ccccatcatg aaactggttg
     6061 aactgatccg cgggcttcaa acgtcggaag aaacgatgga cactgggcgt cgcttcgcca
     6121 cggctgtcgg gaaaacggtg gtcgtctgca aggacagccc tggattcatc gtcaaccttc
     6181 tgctcatacc gtacttgctg gactccgtgc gtgcactcga gctcggaatc gcgaccaagg
     6241 aagacatcga taccgcgatc gtgctcgggc ttgctcatcc aatggggccg ctgacgctgc
     6301 tcgactacgt ggggatcgat acaacgtact tcattgccga ggccatgttc caagagttca
     6361 aggacacccg ctacgccgcg ccgccgctga tgaagcagat ggtgttggcc ggcctccacg
     6421 gccgtaagac cggccgcggc ttctacga
//
LOCUS       DBNGSB010000032         9356 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2168618, whole genome shotgun sequence.
ACCESSION   DBNGSB010000032 DBNGSB010000000
VERSION     DBNGSB010000032.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 9356)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9356)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9356
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2168618"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="AC159B_00925"
     CDS             <1..745
                     /locus_tag="AC159B_00925"
                     /inference="COORDINATES: protein motif:HMM:NF015413.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium transporter MgtE N-terminal
                     domain-containing protein"
                     /protein_id="HIR3376349.1"
                     /translation="EIHPADLAQLMGDLGRDERVEVMTALEHEAAADALAEAEPDVQS
                     DVVKSLPSELAADILEEMSPDDATDVLTDLPAEKVQELIGLMEAEEAKDVRELLKFPE
                     GTAGSVMTTEFIAFAETLTVDETIQRLRGAAPEAEQIYYLYVVDAQQRLVGVLPLRSL
                     ITSPPSQRIGDIMASGVVYLSVTDSVETVASALTKYDLLAMPVLDESLRLSGVVTVDD
                     VMDVVVQKGRRRVPPRFRRRAKPRTKSEV"
     gene            759..2006
                     /locus_tag="AC159B_00930"
     CDS             759..2006
                     /locus_tag="AC159B_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HIR3376350.1"
                     /translation="MWIRERWRRLLLFLAVMGPGVITGNVDNDANGITTYSVAGAEYG
                     YGLLWTLLFSTVTLALVQEMVARMGAVTGKGLADLIRERFRVRLTVLVMSILVLANWA
                     NTVGDFAGVAGSMEIFGISRYLTVPLAALLVGVLVVRGSYRVVERVFLGATLIYVTYL
                     VSAVLADPPWGQVLRATVTPTFQFDAPYLALVVGLVGTTVAPWMQFYQQAAVVDKGIG
                     EKDYRYARLDTYLGVVSLNAIAFFIVVACAATLFTHGVHINDAKDAAVALEPLAGRYA
                     STLFAIGLLNAAVFSVAIIPLSTAYTVCEAFGWEAGLDRSVREAPIFVGLYAGMIALS
                     ALVILVPGVPLIRVMLISQVLNGILLPFVLIFIILLINDRQIMGEYRNSRFVNIASGI
                     TIIVIIILAVLLLGTSARLLLRF"
     gene            2006..2797
                     /gene="recO"
                     /locus_tag="AC159B_00935"
     CDS             2006..2797
                     /gene="recO"
                     /locus_tag="AC159B_00935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HIR3376351.1"
                     /translation="MPVYKAEAIVIRRTSLGEADRLVTLYTRDRGKIATVAKGARKPK
                     SRFAGRLELFTHLRALLAIGKTLDVVSQIEVVDPHAGIRTDLQRMGHAAFIAEVTDRA
                     TADREAAPELFAALRSALHVVAAGEAELAALWYAAQVLVLTGHGPVVDRCVVCGRGVA
                     RGAAFSLALGGALCQADRGRDAQAPSASAVALAAIGFLREAQAAALSKLALEHHHRDE
                     VAGILQRYLEYRLETRLKSPTVIEKMRERDPGKTGTRKRGNAETR"
     gene            2891..3154
                     /locus_tag="AC159B_00940"
     CDS             2891..3154
                     /locus_tag="AC159B_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376352.1"
                     /translation="MEIDLERARRALSQMGSALEEARRVITAGPEGTVRVKTDDKLVE
                     LPISWAVAGAVAFAVAALLSSMPVPSVRRRREREREEEPLGIG"
     gene            3319..4191
                     /locus_tag="AC159B_00945"
     CDS             3319..4191
                     /locus_tag="AC159B_00945"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HIR3376353.1"
                     /translation="MTFQEVIMALERYWSMEGCVIEQPYDLEVGAGTMHPATFLRTLG
                     PEPWRVAFVQPSRRPVDGRYGENPNRLFKHYQYQVVLKPSPDDVQDVYLTSLKKLGID
                     AREHDIRFLEDNWEAPTLGAWGLGWQVFLDGNEITQFTYFQQVGGFDVKPVSVEITYG
                     LERISMYLQRKANVFEIEWAPGITYGEIRHQDEAEQSRYGFEAADVPTLRAFFDASEV
                     EAKRLLALDLVLPAYDYVLKCSHVFNLMDARGAVGVAERTSFMGRCRALARECAERYL
                     ARRDVLGHPLVKTH"
     gene            4193..6304
                     /gene="glyS"
                     /locus_tag="AC159B_00950"
     CDS             4193..6304
                     /gene="glyS"
                     /locus_tag="AC159B_00950"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HIR3376354.1"
                     /translation="MPDLILEVGTEELPPGEIAPALTQLAGGARKLVEDLRLDCGDVS
                     VYGTPRRLVLHVTEVAARQRPAVREVRGPAAQAAYDAHGKPTQAATGFARSQGVPVDR
                     LKLIETAGRRYVAAVVEEAGRPAATVLPDALAALVQGLMFTKTMRWGESDARFVRPVR
                     WVLALFGTRILPVTIAGVRAGRRTYGHRFLSPAARSIGDARAYFSALKRNSVIVDPEA
                     RRNRIVSQARAIAAKEDGRPVLDAALLDELAMSVELPQALLGSFNSEFLSLPRPVLAT
                     VMEHHQKYFPVEDRGAPRAARSASTALLPNFIAVRDGGTRHLAVVREGHEWVLRARLA
                     DARFFFEQDRRQPLEAYAPKLGGLSFHAQLGSMADKTRRLVSLADYIAVVLVLDGRTS
                     EVLRRAAALCKADLVTHMVGEFPELQGVIGGIFAELDGEPGEVARAIAEHYRPRQAGD
                     EPPSTRVGALLALADKCDTLAGGIGAGLMPTGSQDPYGLRRAAQGIVDIILNHRLKLA
                     LKPLIARVAEGYGHASDQSGQEAVEFLRQRLRASLIDRGLRYDLIDAALAVSGDDLLA
                     AAARAETLEAWASKREFARLYVAYDRASRILTGAVNGRVDPHRFEDDSERTLHETVRK
                     VGPPVEGAASEGNFTQALNDLVPLTGPVDRLFDAVLIMAPDEEVRANRMALLKEVVDV
                     FRNVADFSKVVMSDEEKTRTK"
     gene            6422..9067
                     /gene="ppdK"
                     /locus_tag="AC159B_00955"
     CDS             6422..9067
                     /gene="ppdK"
                     /locus_tag="AC159B_00955"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HIR3376355.1"
                     /translation="MLFEEGNAKMRDLLGGKGAGLAEMSRIGLPVPPGFTITTEACLE
                     YDRRGRQYPAGLLQEVRDKIKLIEQRTRKGFGDPKNPLLLSVRSGAKFSMPGMMDTVL
                     NLGLNSATCEGLVHATGDARFAYDSYRRFIQMFGNVVLGMKHEHFEDALAAMKHRRGV
                     TQDTDLRAEDLWRLIEEFHATVKERTGEEFPDEPWRQLEMAIRAVFESWNNPRAITYR
                     NFNRIPHDLGTAVNIQTMVFGNMGTDSGTGVAFTRNPATGERKVYGEYLLNAQGEDVV
                     AGIRTPKPIEDLASDMGAAYAQLMDVFALLERHYRDVQDIEFTIENSTLYLLQTRSGK
                     RTAAAAVKIAVDMVREKLITKEEALLRVEPASLEQLLHPRLDPDAKKEVIGRGLAASP
                     GAAWGTVVFDADEAAKLGAESKVILVRPETNPDDIHGLVAAQGVLTSRGGMTSHAAIV
                     ARGMGKPAVVGAESIQIDEEAKQFIADGGATVRGGEVITIDGSVGEIIRGRVPVIEPT
                     LTGEVQNLLAWADEVRTLGVRANADYPRDARKALEFGAEGIGLCRTEHMFMEEERLPI
                     MQQMIMASTEQERRTALDRLLQFQKDDFKGLFRVMGSLPVIIRLLDPPLHEFLPSLEE
                     LLVEVTELRIRGDGQELAGKEALLRRVQTLREFNPMLGLRGCRLGILYPEINEMQVRA
                     ILLAAVELKKQEGIDVIPEIMIPLIGHPNELKVVHEQLVSVAKKVVDESKTAVKYMFG
                     TMVEIPRACLVADQIAEMAEFFSYGTNDLTQTIFGFSRDDAQAKFLHVYLQKGILKDD
                     PFQVLDREGVGEMMRVGTKLGKKTRPDLEVGICGEHGGDPSSVEFCHEIGLDYVSCSP
                     FRVPIARLAAAQARLKEKTAVTRDV"
BASE COUNT         1800 a         2763 c         3063 g         1730 t
ORIGIN      
        1 cgagattcac cccgctgatc tggcgcagtt gatgggagac ctcggccgcg atgagcgtgt
       61 ggaagtgatg actgcgctcg agcacgaggc tgccgcggac gcgttggccg aggctgaacc
      121 cgacgtgcag tcggacgtgg tcaagagcct gccgagcgag ctcgcggcgg acatcctcga
      181 agaaatgtcc cctgacgacg ccacggacgt gctcaccgat ctcccggctg agaaagtaca
      241 ggaactcatc gggcttatgg aggcagagga agcgaaggac gtccgagagc tgctgaaatt
      301 ccctgaaggc accgcgggca gcgtgatgac cacggaattc atcgcctttg ccgagacgct
      361 gaccgtcgac gagacgatcc agcgcctgcg cggcgcggcg cctgaagcag agcaaatcta
      421 ctatttatat gtcgtcgatg cacagcaacg tctggtcgga gtccttccac tgcgcagcct
      481 cattacgtca ccgccgtcgc agcgcattgg cgacattatg gcctccggcg tcgtctatct
      541 atccgtgaca gactcggtcg agacggtcgc aagcgccctc acgaagtatg atctcctggc
      601 gatgccggtc ctcgacgaat ctctgcgtct gtccggcgtc gtcaccgtcg atgacgtcat
      661 ggacgtcgtc gtccagaaag gacggcgcag agtcccacct cgtttccggc gacgggccaa
      721 gccccggacg aagagcgagg tctagcgatc acctcgatat gtggataagg gagcgctggc
      781 gccggctctt gctgttcctt gccgtgatgg gccccggcgt catcaccggc aacgtcgata
      841 acgacgccaa cgggatcaca acctattcag tggcgggcgc ggagtacggc tacggcctcc
      901 tttggacgct gctcttctca acagtgacac tcgcgctcgt gcaagagatg gtagcgcgaa
      961 tgggagctgt gaccggaaag ggcctggccg acctgatccg agagcgtttt cgggtccgct
     1021 tgacggtcct ggtcatgagc attctcgtcc tcgcaaactg ggccaacacg gtcggcgatt
     1081 ttgcgggggt cgccggatcg atggaaatct tcggcatcag ccggtatctc acggtgccgc
     1141 tggccgcact gctggtgggg gtgctggtcg tgcgaggaag ctaccgggta gtcgaacggg
     1201 tctttctcgg cgcgaccctg atttacgtca cctatctcgt ttcggccgtg ctcgcagatc
     1261 cgccgtgggg tcaggtgctt cgcgccacgg tcacgccgac gttccaattt gatgcgccct
     1321 acctggcgtt agtggtcggg ttggtcggca caaccgtcgc cccgtggatg cagttttatc
     1381 agcaggccgc ggttgtggac aaagggatcg gggagaagga ctacaggtat gcgcggctcg
     1441 acacgtacct tggggtggtg tcgctcaacg cgatcgcatt cttcatcgtt gtcgcctgcg
     1501 cggcgacgtt gtttacgcat ggagtccaca tcaatgacgc caaggacgca gcggtcgcgc
     1561 tggaacctct cgccggccgc tatgcgtcca cattgttcgc catcgggctg ctgaatgcgg
     1621 cggtattttc cgtggccatc attccgttgt cgaccgcgta cacagtttgt gaggccttcg
     1681 gctgggaggc ggggctcgac cgctccgtcc gggaggcgcc gatcttcgta ggtctgtacg
     1741 cggggatgat cgcgctgtcc gcgctcgtga ttctcgtgcc cggggtgccg ttgatccgcg
     1801 tgatgctcat ctcccaggtg ctcaacggaa tcttgctccc gttcgtattg atctttatca
     1861 ttttattgat caacgacagg caaatcatgg gtgagtaccg caattccaga ttcgtcaata
     1921 tcgccagcgg tatcaccatc atcgtgatca tcattctggc cgtgctgctt cttgggacgt
     1981 ctgcgcgttt gctgctgcgg ttctgatgcc cgtctacaaa gcggaagcca ttgtgatccg
     2041 cagaacaagt ctcggcgagg cagaccggct ggtgacgctg tacacacgcg atcggggcaa
     2101 aatcgccacg gtcgcaaaag gcgcgcggaa gccaaagagc cgtttcgccg gacggctgga
     2161 gctgttcacc catctgcgcg cgcttctggc catcgggaag acgctcgacg tggtctcgca
     2221 aattgaggtc gtcgatccgc atgcgggcat tcgaacggac ctgcagcgca tgggtcacgc
     2281 cgcgttcatc gccgaggtga ccgatcgggc caccgcggac cgcgaggccg ctccggaatt
     2341 gtttgccgcg ctgcggagcg cgctgcacgt cgtcgcagcg ggcgaagccg agctcgccgc
     2401 actttggtac gcggcgcagg tccttgtctt gaccgggcat ggcccggtcg tggatcggtg
     2461 cgtcgtctgc gggcggggag tcgcgcgggg cgcggcattt agccttgccc tggggggagc
     2521 actctgccag gcagatcgtg gccgcgacgc gcaggcccct tccgcatccg cggtggccct
     2581 ggccgcgata gggtttctcc gtgaagcgca ggcagcggcc ctctcaaagc tggccctcga
     2641 gcatcaccac cgagatgaag ttgcgggcat cctgcagcgt tacctggagt accggctgga
     2701 aacgagattg aagagcccca ccgtgataga gaaaatgcga gagcgggacc caggaaagac
     2761 gggaacaagg aaacggggaa acgcggaaac gcgataagga agaactgcgg gatccccgta
     2821 gtgtcccgtt tccccgtttc cgcgattccc cgtttccgct atggtatagt gtgtgcggag
     2881 gtgatcggaa ttggagatcg acctggagag agcccgccgc gcgctgtcgc agatgggttc
     2941 cgcgcttgag gaggcccgcc gcgtgatcac ggcaggtccc gagggcacag tgcgggtgaa
     3001 gaccgacgat aagctcgtcg agctccccat ttcgtgggct gtcgcggggg ccgtagcgtt
     3061 cgccgtcgcg gcgctgctgt cttcgatgcc ggtgccatcc gtacgccgcc gccgggagag
     3121 agaaagagaa gaggagccgc tcggcatagg ataatctgca caatctatac aatctgcaca
     3181 atcttcacga tccaaggtgc agccaatcga agttatgttt cagcatttag attgtgtaga
     3241 ttatgtagat tgtgaagata gtgtagattc gagggccatc cgcctccaca cggataggct
     3301 cttctaattt tcgcacccat gacttttcag gaagtcatta tggccctcga gcgttactgg
     3361 tcgatggaag gctgcgtcat cgagcagcca tacgacctcg aggtcggagc cggcaccatg
     3421 catccggcca cgttcctgcg cacgctcggc ccggagccgt ggcgcgtcgc gttcgtgcag
     3481 ccgtcgcgcc gccccgttga cggtaggtac ggagagaacc ccaaccgcct gttcaaacac
     3541 tatcaatacc aggttgtgct caagccctct cccgacgacg tgcaggacgt atatctcacg
     3601 agcctcaaga agcttgggat cgacgcacgc gagcatgaca tcaggttcct cgaggacaat
     3661 tgggaagcgc ctacactggg agcctggggt ctcggttggc aggtgttcct cgatggaaac
     3721 gagatcacac agttcacgta cttccagcag gttggcggct tcgacgtaaa gccggtgtcc
     3781 gtggaaatca cctacggcct cgagcgcatc tccatgtacc tgcagcggaa agcgaacgtc
     3841 ttcgagattg aatgggctcc cggcatcacg tatggggaga tccggcacca agacgaagcc
     3901 gaacagtcga ggtatggctt cgaggccgcg gatgtaccta cgctgcgggc gttcttcgac
     3961 gcgtccgagg tcgaggcaaa gcggctgctg gcgctcgacc ttgttctgcc cgcatatgac
     4021 tatgttctaa agtgctcaca cgtgttcaac ctgatggatg cgcgcggcgc ggtcggggtc
     4081 gcggagcgca cctcattcat gggccggtgc cgcgcgcttg cgcgagagtg cgcagagcgt
     4141 tatctcgccc ggcgcgatgt cctgggccat cccctggtga aaacacacta gagtgccgga
     4201 tctcatcctc gaagtcggca cggaagaact cccgccgggg gagatcgcac ccgcgctcac
     4261 tcagcttgcc ggcggtgcca ggaaactggt cgaggatctg cgcctcgact gcggcgacgt
     4321 aagtgtgtac ggcacgcccc ggcgcctggt tctccatgtc acggaggtgg cagcgcggca
     4381 gcgtccggcc gtccgcgaag tccgcgggcc ggcggcgcaa gccgcgtacg acgcacacgg
     4441 aaagccgacg caggccgcga ccggattcgc gcgcagccag ggcgtgcccg ttgaccggct
     4501 caagctcatc gaaacagccg gacgacgcta tgtcgcggca gtcgtcgaag aagctggacg
     4561 tcccgccgcc acggttctgc ctgatgcgct ggcagctctt gtccaagggc tgatgttcac
     4621 gaagaccatg cgctggggtg agagcgatgc acggtttgtc cgccccgtgc gttgggtgct
     4681 ggctctattc ggaacccgca tattgccggt tacgatcgcg ggggtccgcg caggacggcg
     4741 tacctacggt caccgattcc tgagtcctgc cgcgcgatcc attggggatg cgcgggcgta
     4801 tttcagcgca ttgaagagaa acagcgtcat cgtcgatccc gaagcacggc gtaatcggat
     4861 cgtctcgcag gcgcgtgcga ttgccgcaaa ggaagacggc cggcccgtgc tcgacgcggc
     4921 gttgctcgac gaactcgcga tgagcgtcga gctgccacag gcgttgctcg ggtcatttaa
     4981 cagcgagttc ctctcgctgc ctcgacccgt gctggccacc gtcatggagc accaccagaa
     5041 atacttcccg gtggaagacc gcggcgcgcc gcgcgctgcg cgcagcgcga gtacggcgct
     5101 gctgccaaat ttcatcgccg tgcgcgacgg tggcacccgt catctcgctg tggtgcgtga
     5161 ggggcacgag tgggtgctgc gtgcgagact tgcggacgca cgtttcttct ttgaacaaga
     5221 ccgccgccaa ccgcttgagg cgtacgcacc gaaacttggc ggcctctcct ttcacgccca
     5281 actgggctct atggccgaca agacgaggcg gctggtctcc ctcgccgact acatcgccgt
     5341 ggtgctcgtg ctggacgggc ggaccagtga ggtcctacgg cgcgcagcgg ctctgtgcaa
     5401 ggccgacctc gtgacgcata tggtcggtga gtttcccgaa ctgcagggtg tgattggagg
     5461 aatctttgcg gagcttgacg gcgagccggg ggaggtggcc cgcgcgattg ccgagcacta
     5521 ccgtccccgc caggctggtg atgagccgcc ctcgacgcgg gtcggagcgc tgctcgcact
     5581 cgctgataag tgtgacacac tcgccggcgg catcggcgcg gggttgatgc cgacaggatc
     5641 tcaagatccc tatgggctgc ggcgcgccgc acagggtatc gtggacatca tccttaatca
     5701 tcggttgaag ctcgcactga aaccgctgat tgcaagagtg gcggaagggt acggccacgc
     5761 gtcggatcag tcaggacagg aggcggtgga gtttctacgt cagcggcttc gcgcgtcctt
     5821 gatcgatcga gggctgcgct acgatttgat cgatgccgcc ttagcggtga gcggtgatga
     5881 cctgctggct gcggcggcgc gcgcagagac gctcgaagcg tgggcgtcga agcgcgagtt
     5941 tgcccggctc tatgtggcat acgaccgcgc gtcgcggatt ctgaccggcg cagtgaatgg
     6001 gcgtgtggac ccccaccggt tcgaggacga tagcgagcgc acgctgcacg aaaccgtcag
     6061 gaaggtcgga cctccggtcg aaggcgcggc ctcggagggg aacttcacgc aggcgctcaa
     6121 tgatttggtg cctctgacgg gccccgttga ccggctcttt gacgcggtgc tcatcatggc
     6181 tcccgacgaa gaagtcagag cgaatcggat ggcgctgctc aaagaggtcg tcgatgtctt
     6241 tcgaaatgtg gcagattttt caaaagtcgt catgagcgac gaggagaaaa ctaggaccaa
     6301 atgaggtgtc ttgatggcga gagttcacaa ccgtaaggcg cgacagacgg cgaaaggccc
     6361 gcggcctgtc ccgcggagtg tgcgtaagtc cccccgacac aaaccctcgg gccgctggac
     6421 gatgctcttt gaagagggaa acgcgaagat gcgggacttg ctcggcggga agggcgcagg
     6481 gctcgccgag atgagccgga ttgggctacc ggtaccaccg ggattcacca tcacgaccga
     6541 ggcgtgtctc gagtacgatc ggcgcgggcg ccaatatccg gccggccttc ttcaagaagt
     6601 gcgcgacaag atcaagctca tcgagcagcg aacccgcaag ggcttcggcg atccaaagaa
     6661 cccgctgctg ctttcggtcc gctccggcgc taagttcagc atgccgggga tgatggacac
     6721 ggtgctgaac ctcggtctga actccgccac gtgcgagggc cttgtacacg cgaccggcga
     6781 cgcgcggttc gcctacgatt cgtaccggcg gttcatccag atgttcggca atgtggtcct
     6841 ggggatgaag catgagcact tcgaggatgc gcttgcggcg atgaagcacc gccgcggtgt
     6901 gacccaggac acggacctgc gcgctgaaga tctgtggcgg ctcatcgagg aatttcacgc
     6961 caccgtcaag gagcgaaccg gcgaggagtt tccggatgaa ccctggcggc agctggagat
     7021 ggcgatccgc gccgtgttcg aatcctggaa caaccccagg gcgatcacgt atcgcaactt
     7081 caacaggatt ccccacgatc tcggcaccgc ggtgaacatc cagacaatgg tgttcgggaa
     7141 tatgggtaca gactctggta cgggtgtcgc gttcacccga aacccggcga cgggagagcg
     7201 gaaagtctac ggcgagtacc tcctcaatgc gcagggtgag gacgtcgtcg ccggcattcg
     7261 cacgcccaag ccgatcgagg acctcgcgtc ggatatgggc gccgcctacg cgcagctcat
     7321 ggacgtgttt gcgctgctcg agcgtcacta ccgggatgtc caggacatcg aattcaccat
     7381 cgaaaacagc accctctatc tgctgcagac acgcagcggc aaacgcaccg ccgcggcagc
     7441 ggtgaagatc gcggtagaca tggtacgcga gaagctcatt accaaggaag aggcccttct
     7501 gcgtgtcgag cctgcatcgc tcgagcagct gctccaccca cgcctggatc cggacgcgaa
     7561 gaaagaggtc atcgggcgtg gacttgcggc aagtcccggc gctgcctggg gcacggtggt
     7621 attcgacgcg gatgaggccg caaaactcgg cgccgaaagc aaagtgattc tggtgcggcc
     7681 tgagacaaat ccagacgata tccacgggct ggtggccgca cagggtgtgc tgacgagccg
     7741 cggcgggatg accagccacg cggccatcgt cgcgcgcggg atgggcaaac cggccgtggt
     7801 cggagcggaa tcgatccaaa tcgacgagga ggcgaagcaa ttcatcgccg acggtggcgc
     7861 gacggtgcgc ggcggcgagg taatcacgat cgatggcagc gtcggagaga tcatcagggg
     7921 cagggtgcct gtcatcgagc ccacgctcac cggcgaagtc cagaatttgc tcgcctgggc
     7981 ggacgaagtc cgcactctgg gcgtacgcgc caacgccgac tacccccgcg acgcgcgcaa
     8041 ggcgctggag ttcggcgccg aggggatcgg gttatgccgc acggagcaca tgtttatgga
     8101 agaagagcgg ctgccgatca tgcagcagat gattatggcc tcgaccgagc aggagcgccg
     8161 gacggcgctg gacaggctgc tgcaatttca gaaagacgat ttcaaaggcc tcttccgagt
     8221 gatgggaagc ctgcccgtca tcattagact tctggaccca ccgctccatg agtttctccc
     8281 gagtctcgaa gagttgctcg tcgaggttac cgagctgcgg atccgcggcg acggccagga
     8341 actggcaggg aaagaagcct tgctgcggcg cgtccagaca ctgagagagt tcaacccaat
     8401 gctcggtctg cgggggtgtc gcctcggcat cctatatccc gagatcaacg agatgcaggt
     8461 gcgggccatc ctcctcgcag cggtcgagct caaaaagcaa gaagggatcg acgtcatccc
     8521 tgagatcatg atccctctga tcggacatcc caatgaactg aaggtagtgc acgagcaatt
     8581 ggtgagcgtc gccaaaaagg tcgtcgatga gtccaagacg gcggtgaagt acatgttcgg
     8641 aacgatggtt gagatcccac gcgcgtgcct ggttgcggat cagatagcgg agatggccga
     8701 gttcttttcc tacggcacca acgacctcac gcagacgatc ttcggcttca gccgcgacga
     8761 cgcacaggcg aagttcctcc acgtgtattt gcagaagggt atcttgaagg acgacccgtt
     8821 ccaggtgttg gaccgcgaag gggtggggga gatgatgcgc gtcgggacca agcttggaaa
     8881 gaagacgcgg ccggacctcg aggtcggtat ctgcggcgag cacggcggcg accccagctc
     8941 agtcgaattt tgccacgaga tcgggctgga ctatgtgtcg tgctcaccct tccgcgtccc
     9001 tatcgcccgc ctcgcggcgg cgcaggcccg gctgaaggag aagaccgcgg tgacacgaga
     9061 cgtctgagtt gcccgtagct tgcggggacc ggggaccggg actaggacct gaagatcggc
     9121 taaggagtga cttattacag gtcctttgtc ccgatccccg gtccccgctt cgctcatttc
     9181 taacatcatg aatcctaggg ggaaccgagc ggcgtgatgg ggaccttgga gaaaggggac
     9241 ccgtaactaa gtcactccat agccgattgc cgggtccccg gtccaaggtc cccgtcgcgc
     9301 cgccaaccgt gaattcaaga gaagagatag aacaaagaga acgcgaatgg ctgagc
//
LOCUS       DBNGSB010000033         4945 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_1709234, whole genome shotgun sequence.
ACCESSION   DBNGSB010000033 DBNGSB010000000
VERSION     DBNGSB010000033.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 4945)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4945)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4945
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_1709234"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="AC159B_00960"
     CDS             <1..183
                     /locus_tag="AC159B_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376356.1"
                     /translation="ASILVAMYDGLMLQEGMTSARVDWARVVATLRQGLGTGILTSEA
                     LANSTWTNSGKNEGMS"
     gene            180..1478
                     /locus_tag="AC159B_00965"
     CDS             180..1478
                     /locus_tag="AC159B_00965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3376357.1"
                     /translation="MRRWMVVVLVILAIVVMGGVLASRRRPAAQPPQSQPVTPAPIET
                     VVVEVTSVRRGTIERTVDLTGSIIAAQEVHVTPKIPGKIAVISVTEGSRVSRGQVIAR
                     LEAVELVAQVAQAEQSVRQARAGQELARARLAATVSGARSQERALAENAVRQAEANLK
                     NAEAEAARMQQLFDGGAVSRQQVDAAVLARDVAKTQLEAARQQMSLVQTGARPEDVRM
                     AQAQVAQADAAYGAAVAALNLTRAQLSGATVRAPFSGRVAEIPVSLGEFVSPGMNIAT
                     LYDDQRLEVEVAVGERDLQLVRPGAVVTVTPEALPGRKVAGAVRLVLPAADPASRSAK
                     VRIRLIDPPSGLLPGTFVRAAIIVERHVGALVIPRQALRGSNEVAVVQGGVVRLRTVT
                     VGLQHGALVEVTSGLREGEQVVVLGPETLSDGQPVRTVAH"
     gene            1478..4645
                     /locus_tag="AC159B_00970"
     CDS             1478..4645
                     /locus_tag="AC159B_00970"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3376358.1"
                     /translation="MSLSALSIRRPVLVLMLIAFLAVMGLISYFRLPVELFPSVQFPF
                     VTILTSYPGAGPEEVETLISRPLEEQVSSLTNVRRVVSTSAEGSSIVGIEFNLGTDLE
                     AVTADIRQRVDLVKGSFPRDADDPTVQKFDFSTQPIMLLGMGGRLGGFELRRLAENIV
                     KPRFERVPGVAAVNITGGRTREIRIEVSQDGLRAHGISGLDIVTALQRENLNVPSGII
                     RQGQQEFLVRVVGEFKTLDEIADMRLARLGAAPVRLGDLAVIRDTFKDPDQLTRLDGQ
                     ESVGIGIQKQAGANTVSVADGVTAAIAQLKPALPPGVDIAVARDDSLMIRDSVNDVEG
                     NLILGSILATVVVFLFLHNIRSTVIIALALPAAIVSTYILMYFAGFSLNIISLLGLAI
                     AIGLMIDNAIVVNENINRHLEEERDPKTAAHRGAAEIELAVLASNLANVAVFIPIAFM
                     RGLVGQFFRQFGLTVVFANLFSILIGFTLTPMLSSRWLRPSSDGVGETGIRGRVNRFF
                     ARWDRAYDRLGARYHGALSWALLHRGRVLLIAAVLFIGSIALVASPLIGKEFFPQGDF
                     GQFSVDISLPPGVSLAQTDRATAAVEGQIAPLKEVDASFTIVGGSDTGFGFTEVGTHR
                     AQIFVNLVPREQRSRSHEEVMADLRVRVRNVAAGTIKIGAQGVAGGAAPVMIEVTGPD
                     PAVLERLASQVKDIVAATPGTTNVDTSLRTGKTEVQVRLDRARLALFGLSTADIGSQL
                     RTNIEGTPMTHYRVAGDEFDITVRAQTADRDALEKLGEMRVGAVNGASIQLSDVATLQ
                     LRRGPVTIDRLNRQRLVNVYASLAGRPLGAVVSEIQAKLRAVTVPPAYSVRFGGEAEF
                     QQQAFGDMFFALALGMLLVYMTIAAQFESLRNPLAIMFSVPLAGIGVFLILLISRTTI
                     NLMSLLGIIMLAGIVVNNAILLIEFTTQLRARGLARREALLQAGQTRLRPILMTALVS
                     IMGGLPVALGLGASGAEIRRPLGMAVLGGMFTSTFLTLFVIPVMYTVLEDLTLRVRRR
                     RAVPGGAVQPVAGASNGESGSQQSG"
     gene            complement(4694..>4945)
                     /locus_tag="AC159B_00975"
     CDS             complement(4694..>4945)
                     /locus_tag="AC159B_00975"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR3376359.1"
                     /translation="GGISGVLAFYILRFPKARLGILFRVFFYFRWIQMPAYAWGLLWV
                     VFQLLGAFQQVSGTSNVSALAHLGGAAVGVALWWATQNR"
BASE COUNT          835 a         1539 c         1684 g          887 t
ORIGIN      
        1 gcgtcaattc tcgtcgcgat gtatgacggc ctgatgctgc aggaagggat gaccagcgcc
       61 agggtcgatt gggcgcgagt ggtcgcgacg ctgcgacaag ggctcggcac gggaattctg
      121 acttcggaag ccctggccaa cagtacgtgg actaactcag ggaagaacga ggggatgtca
      181 tgagaaggtg gatggttgtc gtgctggtga ttctcgccat cgtcgtgatg ggaggtgtgc
      241 tggcctcacg gcggcgcccc gccgcgcagc cgccccaaag ccagcctgtg actccggctc
      301 cgatcgaaac ggtcgtcgtc gaagtcacgt ccgtgcggcg aggcacgatc gagcgcacgg
      361 tggatctcac cgggtcgatc atcgccgcgc aggaagttca tgtgacgccc aagatcccgg
      421 gtaagatcgc cgtaatttcg gtgactgagg gttcgcgtgt gagccgaggc caagtcatcg
      481 cgagattgga agcggtcgaa ctggttgccc aggtggccca ggcagagcaa tccgtcaggc
      541 aggcacgggc cgggcaggag ctcgcgcggg cgcggcttgc tgcgacggtg agcggggcgc
      601 gatcccagga gcgcgccctg gccgagaacg cagtgcgtca ggcagaagcg aatctgaaaa
      661 atgccgaagc cgaggcggcg cggatgcagc agcttttcga cggcggcgcc gtgagccggc
      721 aacaggtgga cgcggccgtg ctggcgcgcg atgtcgcgaa aacgcagctg gaagctgccc
      781 gacagcagat gagtttggtg cagaccggcg cgcgccctga ggatgtgcgg atggcgcagg
      841 cgcaggtcgc gcaggccgat gccgcgtacg gcgcggcggt ggcggcgctc aatctgacgc
      901 gcgcacaact cagcggcgcg accgtccgcg caccgttttc gggccgtgtc gcggagatcc
      961 cggtcagtct cggcgagttc gtgtcgcctg gaatgaacat cgcaacgctg tatgacgatc
     1021 aacggcttga agtcgaagtc gcggtcggag agcgcgacct gcagctcgtg cgtcccggcg
     1081 ccgtcgtcac cgtgacgccc gaggcactgc ccggccgcaa ggtggctggg gccgtccggc
     1141 ttgtgctccc cgccgccgac ccggcgagcc gctccgcgaa agtgcgtatc agattgatcg
     1201 atccgccttc ggggctgctc cccgggacgt tcgtgcgcgc cgcgatcatc gtggagcggc
     1261 acgtcggcgc cctggtgatc ccacggcagg cactgcgcgg gagcaacgag gtggcagtgg
     1321 tgcagggtgg cgtcgtgcgc ctgcgcacag tgacggtcgg attgcagcac ggagcgctcg
     1381 tcgaggtcac gtcgggactg cgtgagggtg agcaggtggt tgtgctgggt ccggaaacgc
     1441 tgagcgacgg acagccggtc aggacggtgg cgcactgatg tcgctgtcgg cgttatcgat
     1501 ccgcaggccg gttctcgtgt tgatgctcat cgcgttcctg gcggtgatgg gactcatttc
     1561 ctacttccgc ctgccggtgg aactattccc gagcgtgcag tttccgttcg tcacgatcct
     1621 cacgtcgtat ccaggcgccg ggccggaaga ggtcgagacg ctcatcagca ggccgctcga
     1681 agagcaggtg agttcactca cgaatgtgcg gcgggtcgtc tcgacctccg ccgagggttc
     1741 ctcgatcgtc ggcatcgagt tcaacctggg gacggatctt gaagcggtga ccgccgacat
     1801 ccgccagcga gtggatctcg tgaaagggtc cttcccacgg gacgccgacg acccgaccgt
     1861 ccagaagttt gatttcagca cacagccgat catgttgttg gggatggggg gccggctcgg
     1921 tggatttgaa ttgcgccggc tcgccgagaa tatcgtcaag ccacggttcg agcgcgtgcc
     1981 gggcgtggcg gccgtcaaca tcaccggtgg ccgcacccgc gaaattcgga tcgaagtcag
     2041 ccaagacggg ctgcgggcgc atgggatctc ggggctcgac atcgtcaccg ccctgcagcg
     2101 cgagaacctc aacgtgccca gcggcattat ccgccaaggc cagcaggagt ttctcgtccg
     2161 cgtcgtcggc gagttcaaga cgctggacga aattgccgac atgcgcctcg cgcgccttgg
     2221 cgccgcgccg gtgcgtctcg gcgacctcgc cgtgattcgc gatacgttca aggaccccga
     2281 ccaactcacg cgtctcgatg gtcaagagag cgtgggcatc ggcatccaaa agcaggccgg
     2341 ggccaacacg gtcagcgttg ccgacggcgt gactgccgcg atcgcgcagc tcaagccggc
     2401 gctgcccccg ggggtcgaca tcgcggtggc gcgggatgac tcgctcatga tccgcgactc
     2461 cgtcaacgac gtcgaaggca acctgatcct cggctccatc ctggccaccg ttgtggtgtt
     2521 cctgttcctc cacaacatcc gcagcacggt gatcatcgcg ctcgcgctcc cggcggcgat
     2581 tgtctccacc tatatcctga tgtactttgc cggtttctcg ctcaacatca tttcgctgct
     2641 gggactggcc atcgcgattg gattgatgat cgacaacgcg atcgtggtga acgagaacat
     2701 caaccggcat ctggaagaag agcgtgaccc gaagacggcg gcccaccgag gcgccgcgga
     2761 gattgaactt gccgtgctgg ccagcaacct ggccaacgtt gccgtgttca tccctatcgc
     2821 attcatgcgc ggcctggtcg gacagttctt ccgccagttc gggctgaccg tcgtcttcgc
     2881 caacctgttc agcatcctga ttggctttac gctgacccca atgctgtcca gccgctggct
     2941 ccgcccgtcg tcggatggtg tcggcgagac aggcattcgt ggccgcgtca accggttctt
     3001 tgcgcgctgg gaccgggcgt acgatcgcct cggcgcacgc taccacggcg cgctgagctg
     3061 ggcgctgctg caccggggac gcgtgctgct catcgccgcg gtgctcttca tcggcagcat
     3121 cgcgttggtc gcctcgccgc tgatcgggaa ggaattcttc ccgcagggcg atttcggaca
     3181 gttctcggtt gatatctcgc ttccgcccgg tgtctccctt gcgcagaccg accgcgccac
     3241 cgccgcggtg gaggggcaga tcgcgccatt gaaagaggtc gacgcgtcgt tcaccatcgt
     3301 cggcggttcc gataccggct ttgggttcac cgaagtcggt acccatcgcg cccagatttt
     3361 cgtcaatctc gtgccacggg aacagcgttc gcgctcgcat gaggaagtaa tggccgacct
     3421 gcgggtacgg gtccgcaacg tcgccgccgg aacaatcaag atcggggcgc agggcgtcgc
     3481 cggcggtgcc gcgccggtga tgatcgaggt caccggacct gacccggcgg tgctcgagcg
     3541 cttggcctcc caggtgaaag acatcgtcgc ggccacgcct ggaacgacaa atgtggacac
     3601 gagcctgcgg accggtaaga cagaagtgca ggtccggctg gaccgggcga gacttgccct
     3661 gtttgggctg tcgaccgccg atatcggaag tcagcttcgg acgaacattg aaggcacacc
     3721 gatgacgcac taccgggtcg cgggcgacga gtttgacatc acggtgcgcg cgcaaaccgc
     3781 cgaccgggac gcgctcgaga agttgggcga gatgcgggtc ggcgcggtga acggcgcatc
     3841 cattcagttg agtgacgtgg cgacgctgca gctccggcgc ggtccggtca cgatcgaccg
     3901 gctcaatcgc cagcggctgg tgaacgtcta cgcctccctc gcgggacgcc cgctgggtgc
     3961 ggtcgtctcc gaaatccagg cgaagttgag ggctgtgacc gtcccgccgg cgtatagcgt
     4021 acggtttggc ggcgaggcgg agttccagca gcaagcgttc ggagacatgt tctttgcgct
     4081 ggcgctgggt atgctgctgg tctacatgac gatcgctgcc cagttcgagt cattgcggaa
     4141 cccgctggcg atcatgttca gcgtgccgct cgccggaatc ggtgtgttcc tgattctgct
     4201 gatcagtcgg acgaccatca acctgatgtc gctgctcggg atcatcatgc tggccggcat
     4261 cgtggtcaac aacgcgatct tgctcatcga gtttacgacg cagcttcgcg cgcgcggcct
     4321 ggcgcggcgt gaagcgctgc tgcaggcggg tcaaacgcgc ctgcgcccga tcctcatgac
     4381 cgccctggtg tccatcatgg gaggcctgcc ggtcgcgctc ggcttgggcg caagcggcgc
     4441 ggagatccgg cggccgctcg gcatggccgt gctcgggggc atgttcacgt ccaccttcct
     4501 gacgctgttt gtcattccgg tcatgtacac ggtgctcgag gatctcacgt tgcgggtgcg
     4561 ccgtcgcagg gccgtgccgg gcggagccgt tcagcctgtg gcgggagcgt cgaacggcga
     4621 atcaggtagc cagcaatccg ggtagccagg gagccccaaa agtcttttgg ctcccggatc
     4681 gccggctacc cggctagcgg ttttgcgttg cccaccataa cgccacaccg acggccgctc
     4741 caccgaggtg cgccagcgct gagacgttgc tggtcccgct gacctgctgg aacgcgccga
     4801 gcagctgaaa tactacccag agcagtcccc aggcatatgc cggcatctgg atccagcgga
     4861 aatagaagaa gacgcggaag agaattccca ggcgcgcctt tgggaatcgc aggatgtaga
     4921 atgccagaac ccccgagatc ccgcc
//
LOCUS       DBNGSB010000034         4569 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2476401, whole genome shotgun sequence.
ACCESSION   DBNGSB010000034 DBNGSB010000000
VERSION     DBNGSB010000034.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2476401"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="AC159B_00980"
     CDS             <1..394
                     /locus_tag="AC159B_00980"
                     /inference="COORDINATES: protein motif:HMM:NF021824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative nucleotidyltransferase substrate
                     binding domain-containing protein"
                     /protein_id="HIR3376360.1"
                     /translation="GRLRKEYHGEDRQGVVNLKLRGTLPLVEGTRVLALKAGIPATST
                     LTRLDCLREKGAISAGDHEDLADAFKHITHLLLRQQLEDFKAGRKITNYVPEAHLSQR
                     DKKHLVASFRAIENLRATLNMEFAEHPL"
     gene            complement(523..2628)
                     /locus_tag="AC159B_00985"
     CDS             complement(523..2628)
                     /locus_tag="AC159B_00985"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIR3376361.1"
                     /translation="MQAVVAIPILLAALALAAFAVSGVALWLAVQGGGAVGVYMSIAF
                     AVVAALVGLAWALLHVKLLKPLAALKRELLMQSQIRVDRPLAVDRGHLLNGLPTAVDK
                     ILGALRAARGETQEAVDAATRRTEEQKSRLEAILLDLSEGVIVCNLEHRILLYNQAAA
                     RILNARDTLGLARSLFGVLTREPVLLTLELLLQRSEDAAGDEAQSTGESTLDSTTRRF
                     VCASVDLATLLQARLSLVREPSGRASGYVLTFADVGAELENVAQRDALLREVAMEWRR
                     PLASLRAAAEMLAGELEAGERRVFQDVVGKEVGTLDRSFADVAQRYERLATGQWPLAD
                     IYSLDLFRAVRRHLADADGIQVTPVGVPVWIHADSHSLVLALEHLIRAVARHTAKAAF
                     DIGAVVRDEYVYVEVVWDGAPLASAIIEAWLAEPLKGTIGNRTVRQIVERHGSELWSQ
                     ALPEGRTCLRLPLRKTEQPRAPERSDRVAPMPELYDFDLFAPSTEAIRDTPLRKLNLV
                     VFDTETTGLRPNEGDELISIGAVRVVNGRILTGETFERLINPSRVIPASTTRIHGITT
                     EMVRDKPPAHVILPQFKSFIGDSVLVAYNAAFDMKFLENGAEQAGVKFDNPVLDALLL
                     SVYLQPDVSDFSLTATAERLGVEVIRRHTAIGDAMTTAAIFVKLLDLLKARGIETLGQ
                     AARISSRMMEQRRQQAQLT"
     gene            complement(2649..3023)
                     /locus_tag="AC159B_00990"
     CDS             complement(2649..3023)
                     /locus_tag="AC159B_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3376362.1"
                     /translation="MPQSVLVVDDEANILLSLEFLMKKAGYEVRLARDGEEALAEIGK
                     ARPDLVLLDVMMPKRNGFDVCEAIRANPEWRAVRVILLTAKGRDIEREKGLALGADDY
                     ITKPFSTREVVERVTAWIGPAT"
     gene            complement(3231..3365)
                     /locus_tag="AC159B_00995"
     CDS             complement(3231..3365)
                     /locus_tag="AC159B_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376363.1"
                     /translation="MKKDIWQENKERYFEWNPIESLGAKFTWAAIGAAVAVVAMYLLR
                     "
     gene            complement(4139..>4569)
                     /locus_tag="AC159B_01000"
     CDS             complement(4139..>4569)
                     /locus_tag="AC159B_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HIR3376364.1"
                     /translation="YLNVPPARIPGDGGEQLDDLPADPETIGAALLDAFDRQRQVDLA
                     ARLVARHLSLGHSPQALIATLAHAVLREDAGFHAYQMLEAGVRQFGAWGDTDEGRHIL
                     IAVARYLAAHSPTERASLQTADIARRLMRGGELHQEAGSS"
BASE COUNT          863 a         1448 c         1392 g          866 t
ORIGIN      
        1 cggacgcttg cgcaaggagt accatgggga ggatcggcag ggtgtggtga acctgaagct
       61 tcgtggaacg ctaccgctcg tggaaggcac gcgggtgctt gcgctcaagg ccggcattcc
      121 ggccacatca actctcaccc ggcttgactg cctcagagag aagggggcta tcagcgccgg
      181 agatcatgag gatcttgccg acgcattcaa gcatattacc catctcctgc tgcgccagca
      241 gctggaggac ttcaaggccg ggcgcaagat caccaactat gttcccgagg cacatctctc
      301 gcaacgcgac aagaagcatc tcgtggcgtc cttccgtgcc atcgaaaacc tgcgtgcaac
      361 actgaatatg gagttcgcgg aacacccgct ttagcgcaac tccggacgtt gttatcaagt
      421 gatctgaggc cgcgccaatg gcaccagcga gttgccgctc cgcgtcgtct tggcatgcgt
      481 gtcacctcta tgcgctcttc gctgcgtctc tgcctcacga gatcatgtca gttgtgcctg
      541 ctgacgacgc tgctccatca tccggcttga gatcctggcg gcttgaccga gcgtctcgat
      601 cccgcgggct ttcaacagat ccaggagctt cacgaagatc gcggccgtgg tcatggcgtc
      661 gccgattgcg gtgtgcctgc gaatgacctc gacacccagt cgttcggcag tggcggtcag
      721 cgaaaaatcg gacacatctg gctgcagata gacggacaga agcagggcat cgagcaccgg
      781 gttgtcgaat ttgacgcctg cctgctcggc tccgttctca aggaatttca tgtcgaacgc
      841 ggcgttatag gcgacgagca cggagtcgcc gatgaaggac ttgaactgtg gcaagatgac
      901 atgggcaggg ggcttgtcgc gcaccatttc ggtggtgatg ccgtgaattc gcgtggtgct
      961 ggccgggata acgcggctcg gattgatcag ccgctcgaac gtctcgcccg tcaggatgcg
     1021 accattgacc acacgaaccg ctccgattga gatgagctca tcgccctcgt tgggcctgag
     1081 gccggtggtc tcggtgtcga agacgaccag gttgagcttg cgcaacggcg tgtccctgat
     1141 tgcctcagtc gaaggagcaa acaggtcgaa gtcatatagt tccggcatcg gcgcaacgcg
     1201 gtccgaccgt tccggggcgc gtggctgctc ggtcttcctt agcggcaatc tgaggcacgt
     1261 gcgcccctct ggcagcgcct ggctccatag ctcgctgcca tgtcgctcca cgatctggcg
     1321 gactgtgcgg ttgccgatcg tgcccttcag cggctccgcc agccaggctt cgataatcgc
     1381 cgaggcgagc ggtgcgccgt cccaaaccac ctcgacgtag acgtactcgt ctcgcaccac
     1441 ggcgccgata tcgaaggcgg ctttcgccgt gtgcctggca acggcgcgga tgagatgctc
     1501 gagcgcaagc accagcgagt ggctgtcggc atgaatccag accggcacgc ccacgggcgt
     1561 gacctggatg ccgtcggcgt ctgccaaatg cctgcggacg gcgcggaaca gatcgagcga
     1621 gtaaatgtcg gccaacggcc actgacccgt cgcaaggcgt tcgtaacgct gcgcaacgtc
     1681 ggcaaagctc cgatcgagcg ttccgacctc cttgccaacg acatcttgga agacgcggcg
     1741 ctcgcccgct tcgagctctc ccgccagcat ttcggcagcg gcgcgcaagc ttgcaagtgg
     1801 ccgtcgccat tccatggcga cctcacgcaa gagcgcatcg cgctgggcca cgttttcgag
     1861 ctcggcgccg acatcggcaa aggtcaacac gtagcctgag gcgcggcccg aaggttcgcg
     1921 caccaagctg agcctcgcct gcagcaaagt agcaaggtcg accgacgcac agacgaagcg
     1981 gcgcgtcgtg gaatcgagcg tcgattctcc ggtggactgg gcttcgtcgc cggctgcgtc
     2041 ctctgaccgt tgcaggagca gctcgagcgt caatagcacg ggctctcgcg tcagcacgcc
     2101 aaacaatgag cgggcaagcc caagcgtatc gcgcgcgttg agtatccggg cggcggcttg
     2161 gttgtagagg agtatgcggt gctcgagatt gcagacgatc acaccttcgg agagatcgag
     2221 caggatggcc tcgagccggc tcttctgttc ttccgtacgc cgcgtggcag catcaacggc
     2281 ctcctgtgtc tccccacgcg cggctctgag cgcgccgagt attttgtcga ccgccgttgg
     2341 gagcccattg agcaagtggc cgcgatcgac cgcgagcggt cgatcgacgc gtatctgcga
     2401 ctgcatcagc agctcccgct tcagagcggc caggggcttg aggagcttga catgcagcag
     2461 agcccaagcc agcccaacca aagcagcgac gacggcaaat gcgatggaca tgtaaacgcc
     2521 gaccgctccg ccaccttgca ccgccagcca caacgcaacg ccggacacag cgaaggcggc
     2581 aagcgccaat gctgcgagaa ggattggtat cgcaacgaca gcctgcatcg cacgcccttg
     2641 ttgcccggtt acgtggccgg cccgatccaa gcggtgaccc gttcaacgac ttcgcgcgtc
     2701 gagaacggtt tggtgatgta gtcgtcggcg cccagcgcca agcccttttc gcgttcgatg
     2761 tcgcggccct tggctgtcag caggatgaca cgtaccgccc gccactcagg attggcccgg
     2821 atggcctcgc acacgtcgaa accgttgcgc ttcggcatca tgacatcgag gagcacgaga
     2881 tccggccgcg ccttgcctat ctccgcgagc gcctcctcgc cgtcgcgggc gaggcgaacc
     2941 tcgtagccgg ccttcttcat caggaattcg agggacagca ggatgttggc ctcgtcatcg
     3001 accacgagca cgctttgcgg cattgcatcc tccaggggtc gccttgctcg accgcgcgag
     3061 agcgcggcaa gcgaaaagtg cgctccgccc aactccgcgc cacgtagcta gagcatgatc
     3121 cggaagagct tgccctcgac tggtcggggg tgggtaccgg ttctccgaaa agatcatgct
     3181 caatcaaaaa cctcgagcgc gatcgcgagt cgatctaatc ccgtcgcgct ctagcgcagc
     3241 aggtacatgg cgacgacagc cacggctgcc ccgatcgcag cccatgtgaa tttggcaccg
     3301 aggctttcga tggggttcca ctcgaagtag cgctctttgt tttcttgcca gatatccttt
     3361 ttcatcggtt cctccgtttt cgctctcgcc ctcccaaacc tcagccgagc ccgcccgatg
     3421 acacagcgac ccgccgattc gcgcagcaag tcagactgct ggcatccagg gtcgcgcgga
     3481 ctgaccttac atccttcgcc cctgtttgtc ccttgcccac cttttccgta cctgggtgtc
     3541 cggtaggaat gctgcggtgc cgagcgtgcg aggcagccgc atgggttcga ccttcgttgg
     3601 atcgggcaaa tcgcacgttg caggaacgct cgtttccgct gattgctgca ccgcgacaac
     3661 gcctgcgcct tcgaaaacgt gggccagctg cggttcatct ttggcagcga gcggcttcga
     3721 caccagtcgc ccgatggtta gggccggcgg cctacgaaca ccgtcatgga gcgaggagaa
     3781 aaatggcggc ccggagagat ttgaactcct gaccctcgtt tcgcagtcta atggtgaaag
     3841 gggcgtagct ggcgtagcaa tctggttgag cagcggtaag cctccgatca caattgagtc
     3901 attacaccgc tactaacttc acaccgctac taacttcgtc aattttacgc tgcgactttc
     3961 ctcctgccgc taccatgacg cttccacgaa cgaaatctcc aaccaagttc aggacagcaa
     4021 atccatcgaa aatgatgcgt tttcaaggac ttattgaagg caaactgccg gaagacggtt
     4081 cgtcggcgca ggcttttgtc acttcagatc gcccttagtg cgccctcagc gcgtgacgtc
     4141 aggacgatcc agcctcttga tgtagctcgc caccccgcat caagcggcgg gcgatgtcgg
     4201 ctgtctgcag tgacgcgcgt tcggttggtg aatgggccgc cagatagcgg gcaaccgcga
     4261 tgaggatgtg ccgcccctcg tccgtgtcgc cccacgcgcc gaattgccgg actcccgcct
     4321 ccagcatctg atacgcatgg aagcctgcat cttcgcgcag caccgcatgt gcgagcgtgg
     4381 cgatcagcgc ctgcggcgaa tggccgagcg agagatgccg tgccacgagg cgtgcggcga
     4441 ggtcgacctg tctctgccgg tcgaaggcgt cgagcaaggc ggcgccgatc gtctctggat
     4501 ccgcgggcag atcatcgagc tgctcgccgc cgtcgcccgg gatgcgcgcc ggcggcacat
     4561 tgagatagc
//
LOCUS       DBNGSB010000035         2774 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3243484, whole genome shotgun sequence.
ACCESSION   DBNGSB010000035 DBNGSB010000000
VERSION     DBNGSB010000035.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3243484"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="AC159B_01005"
     CDS             <1..259
                     /locus_tag="AC159B_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020516291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HIR3376365.1"
                     /translation="ARSYWEDARALAAALASGQPPPAVTVPVGFTTFPGEIWASPRSW
                     VEAVYPGLAYFNEVDRGGHFAAWEQPALFASELRAAFKPLR"
     gene            374..886
                     /locus_tag="AC159B_01010"
     CDS             374..886
                     /locus_tag="AC159B_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HIR3376366.1"
                     /translation="MLIAIATLAGVVAFSAPASRHPPQEVTPIFVTKIPPGYRDWKVV
                     SVAHEAGDLNDIRAVLGNDIAIKAYRESKLPFPEGAIVGRIAWSYVSSEENNKAFGRD
                     QSFVAGSPTDFYLQFMVKDSKKYAATGCWGYSSFDKDGKPTDEAAMKKCFPCHQAVKD
                     RDFVFTRYSP"
     gene            1098..2339
                     /locus_tag="AC159B_01015"
     CDS             1098..2339
                     /locus_tag="AC159B_01015"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018900772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase family protein"
                     /protein_id="HIR3376367.1"
                     /translation="MTQTSATQRGREPTTDKTAIRPFQVNIPEAELTELRRRINATRL
                     PEKETVADFSQGVPLATVQKLALYWATEYDWRQVEARLNAVPNFITEIDGLDIHFIHV
                     RSKREKALPVIVTHGWPGSIIEQLKIIEPLTNPTAHGGSSADAFDVVIPSMPGYGFSG
                     KPTTTGWGPERIARAWDVLMKRLGYTSYVAQGGDWGAVIVDLMGVQAPKGLLGIHTNM
                     PGVIPPAIDAAAFAGAPLPAGLSADEKHAYEQLAAFYKDVYYAFFMGTRPQTLTAFAD
                     SPVGLATFMIDHDARSLELIARSFDGVKEGLTRDDVLDNVTLFWLTNTGVSASRLYTR
                     ATGRGWENKLGFFSPKGVKIPVAASAFPDELFQAPKSWTEQAYPKLIHYNKLDKGGHF
                     AAWEQPQLLTEEIRAGFKSLR"
     gene            2520..>2774
                     /locus_tag="AC159B_01020"
     CDS             2520..>2774
                     /locus_tag="AC159B_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR3376368.1"
                     /translation="METTSASAPLPGSSPDEMFPVLTPEQQARVLAHGRLRTAESGET
                     IVEPSTPGIKFFVVVSGRLELHRVSDNKEELIAFCGPGMFT"
BASE COUNT          617 a          825 c          806 g          526 t
ORIGIN      
        1 ggcccggtcg tactgggagg acgcacgagc gctggccgca gcgcttgcca gcggccagcc
       61 tcctccggcg gtcaccgtgc cggtcggctt cacgacgttc cccggcgaga tctgggcctc
      121 cccgcgcagc tgggtcgagg cggtgtaccc cggcctcgcc tacttcaacg aggtcgacag
      181 gggcggacac ttcgcggcat gggaacaacc ggcactcttc gcgtcggagc tgcgggcggc
      241 gttcaagccg ctgcggtgac gcatcgtcag gcggcctaac gcttgttctg aaccttgatt
      301 cgtgatgcca gattacaaat gggttcagtt atcaaatatt cggaggagga tcatcatgaa
      361 aagaaccact tgcttgctga ttgccatcgc aacattggcc ggcgttgtcg ccttcagcgc
      421 gcccgcatct cgacacccac ctcaagaggt caccccgatc ttcgtaacca aaattccacc
      481 aggataccgt gactggaagg tggtctcagt ggctcacgaa gcaggcgatc tcaacgatat
      541 tcgtgccgtt ctggggaacg acatagcgat caaggcatac cgagaaagca agcttccgtt
      601 tccggaaggg gcaatcgttg gccgaatagc ctggagctac gtctcgtctg aggaaaacaa
      661 caaagccttt ggccgtgacc agtctttcgt ggccggctca cccacggatt tttaccttca
      721 atttatggtc aaggactcga agaagtacgc cgcgacgggt tgctgggggt actcgtcatt
      781 tgataaggac ggcaaaccca cggacgaggc ggcgatgaag aaatgcttcc cttgccacca
      841 ggctgtcaaa gatcgcgact tcgtcttcac ccggtactct ccttgatacg gagcgcaaag
      901 aaactgacga agggatgacc acacccaaat ccagaactaa gcgaaagggg cacagcaatg
      961 aaacagcgag aggacagatc gcgccgtcgc tttctcaagg agcgttcgat gttgggtctg
     1021 gccgtcgcgt tcagcccaga aacgatcggc gagggatttg ccgattccaa attcaaaact
     1081 actaaaagga gaacaacatg acgcagacca gtgctacaca acgaggcaga gaaccgacaa
     1141 ctgacaagac cgctattcgt ccgtttcagg tgaacattcc ggaagcggaa ctcacggaac
     1201 tgcgcaggcg catcaacgcg acgcggttac cagagaagga aactgtcgca gacttttcgc
     1261 aaggggtgcc gctcgcgacc gtgcagaaac tcgcgcttta ctgggctacc gaatatgact
     1321 ggcgccaagt cgaggcacga ctcaatgccg tgccgaactt catcaccgag atcgatggtt
     1381 tggacattca cttcatccat gtgcggtcca agcgtgaaaa ggcgctgccg gtcatcgtca
     1441 cgcatggatg gccaggttcg atcatcgaac agctcaaaat catcgagccc ctaaccaatc
     1501 cgacggcgca tggcggcagt tcagcggacg cctttgacgt ggtaatcccg tccatgcctg
     1561 gctatggctt ttcgggcaaa cccaccacca ccggctgggg gcccgagcgc atcgcgcgtg
     1621 cctgggacgt actcatgaag cgtctcggct acacgagtta tgtggcgcag ggcggcgact
     1681 ggggtgcagt catcgtcgac ctgatgggag tgcaggcgcc gaaggggctg cttggcattc
     1741 acaccaacat gcccggtgtc attccgcctg cgatcgacgc cgcggccttt gccggggctc
     1801 cgctgccggc cggtctttcc gcggatgaaa agcatgccta cgagcagttg gctgccttct
     1861 acaaagacgt atactacgcc tttttcatgg gaacgcggcc gcagacgctg acggccttcg
     1921 cggattcacc ggtcggcctg gccaccttca tgatcgacca cgacgcgcgc agcctggagc
     1981 tcatcgcgcg cagcttcgac ggagtcaagg agggtctcac gcgagatgat gtgctcgaca
     2041 acgtcacatt gttctggctg acgaataccg gcgtatccgc gtcgcgactc tacacccgcg
     2101 ccacgggcag aggttgggag aacaagctcg gcttcttctc gccgaagggc gtcaagatcc
     2161 cggtggccgc aagcgccttc ccggatgaac tcttccaggc tccgaagagc tggacggagc
     2221 aggcatatcc caagctcatc cactacaaca agctcgacaa gggtggccac ttcgccgcct
     2281 gggaacagcc gcaactcctc actgaggaga tccgggctgg gttcaaatcg ctgcggtaga
     2341 tctaaatgag tgggaaggag agacccatcg cacgtcaggc ctcggaagca agcgtgagca
     2401 tcagcctggg gcgtcagccc caggttggcg atggcttctc aaaatgcgag cccgctccag
     2461 ctagttgccg tagtaacggc gtgcgctaat ctcatgcaac tctcgcgaca gggccactga
     2521 tggaaacaac atctgcatct gcaccactgc ccggctcttc tcctgacgaa atgttccctg
     2581 tgcttacacc ggagcaacaa gcgcgcgtgc tggcgcatgg ccgattaaga acggccgaga
     2641 gcggtgagac tatcgtcgaa ccgagtacac caggaatcaa gttcttcgtg gtcgtttccg
     2701 gccggctcga acttcaccga gtctcagaca ataaagaaga gctgattgcc ttttgtggac
     2761 cgggcatgtt cacc
//
LOCUS       DBNGSB010000036         4050 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2476812, whole genome shotgun sequence.
ACCESSION   DBNGSB010000036 DBNGSB010000000
VERSION     DBNGSB010000036.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2476812"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="AC159B_01025"
     CDS             complement(<1..803)
                     /locus_tag="AC159B_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013158523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3376369.1"
                     /translation="MRIVHRMLILGLAVALAWTPAVVAQGQKASIIEAWFIHNESMGD
                     PVAVEKGFFGNLQVSVVGGGPGLSPIDRVMAKARAGEIVFGVDYPYNILEAREKQRLP
                     LVVVSHDFQKSAIHLLSWVPLKTARDVRGTVATWIGYDKQIKSMIGRDWSGRIKVVNQ
                     QGDPATIGAWMQKQYQFAHAMGYNEVLVAKRLVKQRYYVYSYTDDFKMNWPENIVFTT
                     EDIIRRYPEAVQQFVSGHYRGFQYAFANRNEAADILLKYNKNLDKAHEIA"
     gene            complement(835..1626)
                     /locus_tag="AC159B_01030"
     CDS             complement(835..1626)
                     /locus_tag="AC159B_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013158524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3376370.1"
                     /translation="MSTAHAARRSPFADLGSWTIFAMFMVAAWEVGSRLYDQPYLLPA
                     PSQILRALGEDYGLVLSYARITMQETVLGFVLGAGAALLGAMLFVWLPRPVEDFLYRI
                     IVTLNSTPFVALASLAVVWFGLGITSKIIIAGMYTFFAVLYHTHKEFISVDPMREYLM
                     DVYNAGWLQRMLLLKIPSALPIIFTSLKGGVMAAVNGAIVGELFGAFEGLGYMILDSR
                     YVGNTVRVFLAAVFCTFIGWVLLGIVTVAERVLLPWHVSIARERM"
     gene            complement(1626..2894)
                     /gene="codA"
                     /locus_tag="AC159B_01035"
     CDS             complement(1626..2894)
                     /gene="codA"
                     /locus_tag="AC159B_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine deaminase"
                     /protein_id="HIR3376371.1"
                     /translation="MDLVIRKATIAGKEGPHDIGIRGERIASITPSIKERGQTEIDAA
                     GNLVTPALVETHIHLDAALTVGQPRHNRTGSLFEGIEIWSERVRILTHEDVKQRATTA
                     LKWLLVHGVTHVRTHVDVCDPKLTALQALLELREEVRDIMTVQIVAFPQQGIYSFPDG
                     EELMVRALEMGADVVGGIPHYEWTREYGERDVRTALRLAAEYDRQADLHCDETDDDQS
                     RFLELVGAETIRLGLQGRVTASHTTAMHSYNNAYANRLIRWIRNAGVHFITNPLDNSV
                     LQGRFDGYPIRRGFTRVKELLASGINVCIGQDSMMDPWYPLGIGDPLQACFVMAHYGQ
                     MSGRDELEMMLDLVTTRAAACFGAEHYGLEEGKQADLVVFDAPTPVDAVRTMAARRWV
                     ISRGRVAAETAPASARVQWNGREEQVRFLV"
     gene            complement(2898..3326)
                     /locus_tag="AC159B_01040"
     CDS             complement(2898..3326)
                     /locus_tag="AC159B_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376372.1"
                     /translation="MSRMIEEHTVLGRTCSVTIEGPDGDRRYNAYVKENASGRMLTRN
                     PVRGRSVDDVRDRALEVMHNLLGIERIQEEVLAVAAEVAPGASVELTEDAQTIRASLT
                     GSWELDVPFTVSRDELEEGLAIESLRLRILEHFHAHLKEP"
     gene            3356..>4050
                     /locus_tag="AC159B_01045"
     CDS             3356..>4050
                     /locus_tag="AC159B_01045"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:NF016084.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HIR3376373.1"
                     /translation="MNPPVVGITIGDPAGIGPEIVVRALAHESVPASVTPVVIGDRWV
                     LERAMDATGIRLEFDSTGRGRLIDLANVDRRLQWGKIQATGGGAAAQFIERAVQEALA
                     GRLDALATAPINKEALWRAGYRHLGHTEMLADLTRSDDPLTMFQVRGLRIFFLTRHLS
                     LREALDQITRERISSMLPRIDNELKRLGITRPTLAVAALNPHAGEGGALGREDQDEIA
                     PAVEDARTRGLSV"
BASE COUNT          753 a         1314 c         1207 g          776 t
ORIGIN      
        1 gcgatctcgt gcgccttatc gaggttcttg ttgtacttca gcaggatgtc cgccgcttcg
       61 ttccggttgg cgaacgcgta ttggaacccg cgataatggc cggacacgaa ctgctggacg
      121 gcctccgggt accgtcggat gatgtcctcg gtcgtgaaga cgatgttttc aggccagttc
      181 atcttgaagt cgtctgtgta cgagtagacg tagtaccgtt gttttacgag ccgcttcgcg
      241 acgagcacct cgttgtagcc catcgcgtgc gcgaactggt actgcttctg catccacgcg
      301 ccgatcgttg ccgggtcgcc ctgctgattc acgaccttga tgcgcccgga ccaatctcgg
      361 ccgatcatcg acttgatctg cttgtcgtag ccgatccagg tggcgacggt gcctcttacg
      421 tcccgcgccg tcttgagcgg cacccacgag agcaggtgga tggccgactt ctgaaagtca
      481 tgcgacacga cgacgagcgg gagccgctgc ttctcgcgcg cctcgaggat gttgtaaggg
      541 tagtccaccc cgaagacgat ctcgcctgcg cgcgccttcg ccatgacgcg gtcgatgggt
      601 gacagacccg gcccgccgcc caccacgctc acctgcaggt tgccgaagaa ccccttttcg
      661 accgcgacgg gatcgcccat cgactcgttg tgaatgaacc aggcctcgat gatcgaggcc
      721 ttttgacctt gggccacgac cgcgggcgtc caggccagcg ccacggccaa cccaaggatc
      781 agcattcggt gcacaatccg catcgctcca ccccctgtat gatttactgc ctcgctacat
      841 cctctcccgt gcaatgctga cgtgccatgg cagcaagacc cgctccgcca ccgtgacgat
      901 gccaagcaac acccatccga tgaacgtgca aaacaccgcc gcgagaaaaa cccgcacagt
      961 atttccgaca tatcgtgaat ccaggatcat gtagcccaat ccttcgaacg ccccaaagag
     1021 ctccccgacg atcgcgccgt tgaccgccgc catcacacct cccttcaggc tcgtgaagat
     1081 gatcggcagc gccgagggaa tcttcagcag cagcatccgc tgaagccagc cggcgttgta
     1141 cacatccatg aggtactcac gcatcgggtc gacagagatg aactccttat gcgtgtggta
     1201 cagcacggcg aagaacgtgt acatgcccgc gatgatgatt ttgcttgtaa tccccagccc
     1261 aaaccagacg accgccagcg acgcgagtgc gacaaacggg gtgctgttca gggttacgat
     1321 gatgcggtag aggaaatcct ccaccggcct cggcagccac acaaacaaca tggcgccgag
     1381 cagcgcggcg cccgcaccaa gtacaaaccc cagcaccgtc tcctgcatgg tgattcgggc
     1441 gtaggagagc acgagcccgt agtcttcgcc cagcgcgcgg aggatctgcg agggcgccgg
     1501 gagcaggtac ggctggtcgt acagccgcga accaacctcc catgcggcga ccatgaacat
     1561 cgcaaagatc gtccacgatc caaggtccgc aaaaggactg cgccgcgccg cgtgcgccgt
     1621 cgacatcaga cgagaaacct gacctgttcc tcgcgtccgt tccattggac gcgggcactc
     1681 gcaggtgccg tctccgcggc gacgcgcccg cgcgagatca cccaccggcg cgcggccatc
     1741 gtgcgtacgg catcgaccgg cgtcggcgca tcgaacacga ccaggtcggc ctgcttccct
     1801 tcttcgagtc cgtagtgctc ggcgccaaaa cacgccgccg cccgggtcgt caccagatcg
     1861 agcatcatct cgagctcgtc gcggccggac atctgcccat agtgcgccat cacaaagcat
     1921 gcctgcagcg gatccccgat gcccagcgga taccaggggt ccatcatcga gtcctggccg
     1981 atgcatacgt tgatgccgct cgccagcaat tccttgacgc gcgtgaaccc gcggcggatg
     2041 gggtatccgt cgaagcggcc ctgcaacacc gagttatcca gcgggttggt gatgaagtgc
     2101 acgcctgcgt tcctgatcca ccggatgagg cgattggcat aggcgttgtt ataggagtgc
     2161 attgccgtcg tatggctggc tgtcactcgt ccctgcagcc ccagccggat cgtctcggct
     2221 cccacgagct ccaaaaaccg gctctgatcg tcgtccgtct cgtcgcagtg gaggtcggcc
     2281 tgccggtcgt attccgccgc cagccgcagc gcggttcgca cgtcgcgctc gccgtactcc
     2341 cgcgtccact cgtaatgggg aatcccgccc accacgtcgg cgcccatttc aagcgcgcgc
     2401 accatcagtt cctcgccgtc agggaacgag tagatcccct gctggggaaa tgccacgatc
     2461 tgtaccgtca tgatgtcgcg cacttcctcg cggagctcaa gcaacgcctg gagcgctgtg
     2521 agttttgggt cgcacacatc cacgtgggtg cggacgtgcg tcacgccgtg gaccagcaac
     2581 cacttcagtg cggtggtggc gcgctgcttc acatcctcgt gggtcaggat gcggacacgc
     2641 tcgctccaga tctcgatgcc ttcaaagagc gatccggtgc gattgtggcg aggctggccg
     2701 accgtcaggg cggcgtcgag gtgaatgtgc gtctcgacca gcgcgggtgt gacgaggtta
     2761 ccggcggcat cgatctccgt ctgtccgcgc tctttgatgg atggggtaat tgacgcgata
     2821 cgctctccgc ggatcccaat gtcgtgagga ccttccttgc cggcgatggt agctttgcgg
     2881 atgacgagat ccatggatta cggttctttc aagtgtgcat gaaagtgttc caggatccgc
     2941 aggcgcagcg actcgatggc cagaccttct tcgagctcgt cccgcgacac cgtaaacgga
     3001 acgtcgagct cccacgatcc agtaagcgaa gcgcgaatgg tttgcgcatc ttcggtgagt
     3061 tccactgacg ctccaggcgc aacctcagct gcaacggcca ggacttcctc ctggattcgc
     3121 tcaatcccga ggaggttgtg cattacctcg agggcccggt cgcggacatc gtccacactc
     3181 cgcccacgaa ctggattgcg ggtcagcatg cggccgctgg cattttcttt tacgtaggca
     3241 ttataacgac ggtcaccgtc tggaccttca atggtgacgg agcacgtgcg tccgagcacg
     3301 gtatgctcct caatcatgcg ggacatgtct gctgaacgct tgtagaatga tctctgtgaa
     3361 tcctcccgtc gtcggcatca ccatcggcga tcccgccggc atcggtcccg aaatcgtcgt
     3421 gcgcgcactt gcccacgaaa gcgtcccagc gtctgtgact cctgtcgtca tcggtgatcg
     3481 ctgggtcctc gaacgggcga tggacgcgac gggcatccgg ctcgagttcg actccaccgg
     3541 ccgagggcgc ttgatcgatc tcgcgaacgt ggatcgccgc cttcagtggg gaaagattca
     3601 ggccacgggc ggaggcgccg cggctcagtt catcgagcgt gcggttcaag aggcgctggc
     3661 cgggcgcctg gacgccttgg ccaccgcgcc tattaataag gaagcattgt ggcgggccgg
     3721 ataccggcac ctcgggcata ccgagatgct ggcggatctc acgcgttcgg atgatccact
     3781 gacaatgttc caggtcagag gcctgcggat tttctttctc acccgacacc tgtcactgcg
     3841 cgaagcgctg gatcagatca cgcgcgagcg tatctcctcg atgcttcccc ggatcgacaa
     3901 cgagttgaag cggctgggga tcacgcggcc gactctcgcg gtggcggcgc tcaatcccca
     3961 cgcgggggaa ggcggcgcgt tgggtcgcga ggaccaggac gaaattgcgc ccgcggttga
     4021 agatgctcgc acccgtggcc tttcagtcca
//
LOCUS       DBNGSB010000037         2732 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2325893, whole genome shotgun sequence.
ACCESSION   DBNGSB010000037 DBNGSB010000000
VERSION     DBNGSB010000037.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2325893"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="AC159B_01050"
     CDS             <1..796
                     /locus_tag="AC159B_01050"
                     /inference="COORDINATES: protein motif:HMM:NF016201.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HIR3376374.1"
                     /translation="LNHAELHARTIRKYQDQYGLEKIDSLLDALLTVAGTVNAFERSS
                     GERRKRLIYHLEEKAPLEEWERHLLQIVREEAEYFDLIQRTHVINEGWATFVEAELLR
                     DILDTKSWASLSVQLSNRPAPYTIGYTLFKRIKGERGFGAALGVRTYYEDIRLIDEIL
                     TDEMVRRLDIFVYDPKEKKKSYDLQQVKEMLISQKLHKGEPHIEVEPGAHHRDLILIH
                     RDEERKLDAKRVGLFLKAVHSLWRNPVRLRANGKTYSYDRRGLSTS"
     gene            complement(871..1338)
                     /locus_tag="AC159B_01055"
     CDS             complement(871..1338)
                     /locus_tag="AC159B_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HIR3376375.1"
                     /translation="MKVRGVALDQQMNPVVLLVDQSETLALPIWIGQAEATAIAMELQ
                     GVKAPRPMTHDLLRAVLQTLAISVTRIVVTEVKDSTYFAEIHLAGNGKPLIIDSRPSD
                     AIALALRSEAPIFVADNVAEQAIALKKPLEEEMEEFKKFLESVKPSDFKGTVH"
     gene            1640..2287
                     /locus_tag="AC159B_01060"
     CDS             1640..2287
                     /locus_tag="AC159B_01060"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HIR3376376.1"
                     /translation="MRQFLITVCISITTTLALVPIADAAAQTHVVRRGDTLSSIARRH
                     GVSAAMLAAINDLANPDRLYPGQRLAIRRTTNLPPHRRVVTASAQPSRGQKWGSALLA
                     SAFRHIGVRYVWGGMSPRGFDCSGFIGFVMRSVGVLLPRTAAELYVSGRPVPTDQLRV
                     GDIVFFETTRPGPSHAGIYIGHDQFIHASSGFGRVTVTSMDYPYYKPRYMGARRF"
     gene            2514..>2732
                     /locus_tag="AC159B_01065"
     CDS             2514..>2732
                     /locus_tag="AC159B_01065"
                     /inference="COORDINATES: protein motif:HMM:NF019850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HIR3376377.1"
                     /translation="MPGRVARSVAPDVIRTLAARLPSGTVVVTGTNGKTTTSRLIAHI
                     LRLAGLSSVHNRAGANLAAGIAAALLQHA"
BASE COUNT          534 a          822 c          817 g          559 t
ORIGIN      
        1 gctcaatcac gccgagctgc acgcgcgcac gatccgcaag taccaggatc agtacgggct
       61 cgagaagatt gattcgctcc tcgacgcgct gctgacggtg gcggggacgg tcaacgcatt
      121 tgaacgcagc tcgggtgaac ggcgcaagcg tctgatctac catctggaag agaaagcgcc
      181 gctggaagag tgggagcggc acttgttgca gatcgtccgc gaagaggccg agtactttga
      241 cctgattcaa cgcacgcacg tcatcaacga agggtgggcg accttcgtcg aggcggagct
      301 gctgcgtgac atcctggata ccaagtcatg ggccagcctg tcggtacagt tgagcaaccg
      361 tcccgcgccg tacacgatcg gctacacgtt gttcaaacgc atcaagggcg agcgcgggtt
      421 cggcgcggcg ctgggcgtgc gcacgtatta cgaggacatc cgtctcatcg acgagatcct
      481 caccgacgag atggtgcggc gcctggacat ctttgtctac gatccgaaag agaaaaagaa
      541 gagctacgac ctgcagcagg tgaaggaaat gctcatcagc cagaaactgc acaagggcga
      601 gccgcacatc gaagtcgagc ccggcgccca ccaccgcgac ctgatcctga tccaccgcga
      661 tgaagagcgc aagctggatg cgaaacgcgt cggcctcttt ctcaaggccg tgcactcgct
      721 gtggcgaaat ccggtgcggt tacgagcaaa cgggaaaacc tattcgtacg atcgaagagg
      781 gctctctaca agctgactgc ggtcatgggt cgtagatcgt cgcaactgcc ttcctatgac
      841 cgacgaccaa tgacctacga ccgctgttag ttagtgtacg gtgcctttga agtctgaggg
      901 cttcaccgac tccaggaact tcttgaattc ttccatctct tcttccaggg gcttcttcag
      961 cgcaatggcc tgctctgcga cattgtcggc cacgaagatc ggcgcctctg aacggagggc
     1021 cagcgcgatg gcatcgcttg gccgcgagtc gatgataagg ggcttgccgt ttcccgcgag
     1081 gtgaatctcg gcgaagtacg tgctgtcttt gacttcagtg acgacgatcc gggtcacact
     1141 gatcgcgagg gtctggagaa cggcgcgcag gagatcgtgg gtcatgggtc gcggcgcctt
     1201 cacaccctgc aactccatcg cgatcgcggt cgcctcagct tggccgatcc agatggggag
     1261 cgcgagggtc tcgctttggt ccactaacaa cacgacggga ttcatctgct ggtccagcgc
     1321 cacgccgcgg actttcatgt gcaccacgag atgtcccctc cttcgtgcgc cctattatag
     1381 cagaatcatt ttgcggtcaa gaaacgcata tgctgcgtac ccgattcccg accgcttagt
     1441 cccgtcgtaa tttgtttttc ccgcatcgat caggtcacgg gagggggaat cagtgacccg
     1501 ggcgaataag ccgtgagcgc tcgagcggcg tctatgcttg caagggcgcg ggtatcactc
     1561 tcacgtttcg tggatccggc aagcaccaac ggcatcttca attcttcggc gacggaagcc
     1621 ggatactacg gagggcaacg tgaggcaatt cctgattaca gtttgtattt cgatcaccac
     1681 cacgctcgca ctggtgccca tcgccgatgc agcggcacaa acgcacgtcg tcaggcgggg
     1741 cgatactttg tcgtcaatcg caaggcgaca cggcgtgtcg gcggcgatgc tcgctgccat
     1801 aaatgatctc gcaaatcctg atcggttgta tcctggtcag cggcttgcga tccgccgcac
     1861 gacgaacctc cctccgcaca ggcgcgtcgt gacggcgtct gcccagccat ctcgaggtca
     1921 gaagtggggt tcagcgctac tcgcctcagc cttcaggcat ataggcgtcc ggtacgtatg
     1981 gggcgggatg agcccccgcg gcttcgactg ctcgggattc atcggttttg tgatgcgatc
     2041 ggtcggcgtt ctgctccctc gaaccgccgc agagctctat gtctcgggcc ggcctgtgcc
     2101 cacggaccag ctgcgtgtcg gtgacattgt gttcttcgaa acaacgcgtc cgggcccgag
     2161 ccatgcgggc atctacattg gccacgatca gttcatccat gcctcgtctg gatttggccg
     2221 tgtgacggtg acctcgatgg actatccgta ttacaagccc cggtacatgg gagctcggcg
     2281 cttctaactc cctaaccacg ggaacaaagc gaacgaagcg atagaggcga accaggaaac
     2341 tgcatcgttg cgtctcgtga ccttgttcgc tttgttcgcc ttgttcgctg tgttcgcgtc
     2401 ttcccaaagg gtatgatcca cttgtgaagt ctctgcgtct aattgtcgcg attgcccttg
     2461 gcaaaatcac ggcagttggg agccggttgc ttggttgagg tggagggacg acgatgccgg
     2521 ggcgcgtggc gcgcagtgtc gcccccgacg tcatccgaac cctggcggcc cgcctgccct
     2581 ccggcacagt tgtcgtcacg ggaaccaacg gcaagacgac gacctcgcgt ctaatcgcac
     2641 acatcctccg ccttgcgggg ctctcatccg tgcacaatcg cgccggcgcg aacctcgccg
     2701 ccggcatcgc tgccgccttg ttgcagcacg cg
//
LOCUS       DBNGSB010000038         3242 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_792684, whole genome shotgun sequence.
ACCESSION   DBNGSB010000038 DBNGSB010000000
VERSION     DBNGSB010000038.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_792684"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="AC159B_01070"
     CDS             <1..232
                     /locus_tag="AC159B_01070"
                     /inference="COORDINATES: protein motif:HMM:NF013768.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HIR3376378.1"
                     /translation="ADLLGAVSARQVRITIGSEHRYEDLRGCSLIAAAYRIGEGAVGA
                     LGILGPTRMNYAKVISLVRYLTSELSATPEQS"
     gene            238..1341
                     /gene="dnaJ"
                     /locus_tag="AC159B_01075"
     CDS             238..1341
                     /gene="dnaJ"
                     /locus_tag="AC159B_01075"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006696716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HIR3376379.1"
                     /translation="MRDYYDVLGLDHSASQDEIKRAFRRLAREHHPDVKKNDPQADER
                     FKEINEAYQVLSDPQRRSQYDQFGTVSPTYAAEEFRDFGLGSFNDIFDMFFGRRGAGV
                     DRDAPIRGTDLRYDVEISLEEAARGVEREIEIERLDTCPSCFGTGAERGSAPQACPTC
                     RGSGQQRYSQRTALGSFTQIITCTQCGGAGTVLRHPCKQCRGSGRTPNKKMLTVTIPK
                     GVDSGMRLRLAGEGEGGERGGERGDLYVFLDVRPHPTFERRGNDLFCEIPISITQAAL
                     GDEVQVPTLDGPAVHAVPAGTQPDDVVTIQGKGMPDPRGGRGDLHARLHVTVPTNLSA
                     EERRLLEELAKLRGEQIKPQKKKLADKVKEMLQ"
     gene            1371..2240
                     /gene="prmA"
                     /locus_tag="AC159B_01080"
     CDS             1371..2240
                     /gene="prmA"
                     /locus_tag="AC159B_01080"
                     /inference="COORDINATES: protein motif:HMM:NF018076.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HIR3376380.1"
                     /translation="MKWVEITVTTAPQAVEAIANILLESRTGGIVEESPQPGLTLLRG
                     YLPVGPATTAKLNAICARVQALKAYGLDAGPARIETEIVEESGWAQAWKSHFKPFAVG
                     KRLWISPTWDETAPPPEAVIVALDPGMAFGSGLHASTQMCLSVIEEHIRGGEHVFDVG
                     TGSGILSIAAAKLGAAVVLAIDDDPVAVEVTGQNVQHNRVAGRVTVRLGDLLDGVADK
                     ADLILANLTADIHLKFLADGPSHLTSGGILAASGIVEDRMPEVEAVARSVGLRSLEVK
                     RDAEWRCLVLTRS"
     gene            2286..3032
                     /locus_tag="AC159B_01085"
     CDS             2286..3032
                     /locus_tag="AC159B_01085"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HIR3376381.1"
                     /translation="MHRFSLSETISGDRVVLRDAQARHASVVLRVRPGERIVVFDGSG
                     REFDVELESVDARAVTGRIVATRKAGRFPLHITLLQGVPKGAKMDDVIRMGTEIGVAE
                     FVPFLSERTVAEGRQRADRWRRVATEAAKQSGRSDVPVVHDPSPLTQALEGLAGHDLL
                     LVPWEAERHRSIRDVLEQSDRVSRIAIAIGPEGGLEAREIEEAARFGGVAVTLGPSVL
                     RTETAGIVAVSMVLYGLVMRTTGRGESAPA"
BASE COUNT          625 a          939 c         1113 g          565 t
ORIGIN      
        1 cgccgacctg ctgggcgccg tttcggcgcg gcaggtgcgg atcacgattg gcagcgagca
       61 ccgttacgag gacttacggg gctgcagcct catcgctgcc gcataccgga tcggggaggg
      121 cgcggtaggc gcgctcggca tccttggtcc aacccggatg aattatgcga aagtgatctc
      181 gctcgtgcgg tatcttacct cggaactcag cgcaacgccg gagcagtcat agcgatcatg
      241 cgcgactatt atgacgtcct tggcctcgac cacagcgcct cgcaggacga gatcaaacgt
      301 gcctttcgcc ggctggcacg tgaacaccat cctgacgtga agaagaacga tccgcaggcg
      361 gacgagcgat tcaaagagat caatgaagcc taccaggtcc tgtctgaccc acagcggcgg
      421 tcgcagtatg accagtttgg gacggtgtcc ccaacgtacg ccgccgaaga gtttcgagac
      481 ttcgggctcg gatcgttcaa tgacattttc gacatgttct tcggccggcg tggggcgggg
      541 gtggaccgag acgctcccat acgcgggacg gaccttcggt acgacgtgga gatctcgctg
      601 gaagaagccg cccgcggagt tgaaagagaa atcgagatcg agcggttgga cacctgcccg
      661 tcgtgctttg gaaccggcgc cgaacgcgga tccgcgccgc aggcctgtcc gacctgtcgg
      721 ggcagcggcc agcagcggta ctcgcagcgc acggcgcttg gctcttttac acagatcatc
      781 acctgcacgc agtgcggtgg agcgggcacc gtcctgcgcc atccctgcaa acaatgccgc
      841 ggcagcggcc gcacgccgaa caagaagatg ctcacggtca cgatcccgaa aggcgtcgat
      901 agcggtatgc ggcttcggct cgcgggggag ggtgaaggcg gcgagcgtgg cggggagcgc
      961 ggtgacctct atgtgttcct cgacgtgcgg cctcatccca cgttcgagcg acgggggaac
     1021 gatctctttt gtgagatacc gatctcgatc acacaagcgg ccctcgggga tgaagtccag
     1081 gtgccgacgc tggacggccc cgcggtacat gcggtgccgg ccggtacgca gccggacgac
     1141 gtggtgacga tccagggcaa agggatgccg gatccgcgcg gcgggcgagg agatctccac
     1201 gcgcgcctcc acgtcacggt ccccacaaac ctgagcgcgg aggagcggcg gctgctggaa
     1261 gagcttgcga agctccgggg cgagcagatc aagcctcaga agaagaaact cgccgataag
     1321 gtcaaggaga tgttgcagta ggcgcgcagc gcgcggcgcg ccgcgcgaac atgaagtggg
     1381 ttgaaatcac cgttaccact gctccgcagg ccgtcgaggc gatcgccaac atcttgctgg
     1441 aaagccggac gggcgggatc gtcgaggaat ctccccagcc tggcctcacg ctgcttcgcg
     1501 gctatctgcc ggttggaccc gccacgacgg ctaagttgaa cgccatatgc gcgcgcgtcc
     1561 aagccctcaa ggcatacggg ctcgacgcgg ggcctgctcg cattgagacg gaaatcgtcg
     1621 aggaatcggg gtgggcgcag gcgtggaaga gtcatttcaa gccctttgcg gtaggcaaga
     1681 ggttgtggat cagcccaacg tgggatgaga ccgctccgcc accagaggcc gtcatcgtgg
     1741 cgctcgatcc tgggatggcg tttggcagcg gcctgcatgc gtccacgcaa atgtgtctgt
     1801 ccgtaatcga ggagcacatc cgtggcggcg agcacgtctt cgacgtcggc acaggatcgg
     1861 ggattctctc tatagctgcg gcgaagctgg gcgccgcggt cgtcctggcg atcgacgacg
     1921 atccggttgc cgtagaggtg accggccaga acgttcagca caatcgcgtg gcgggtcggg
     1981 tcaccgttcg cctgggcgac ctgctggacg gcgtcgcgga caaggccgat ctgattctcg
     2041 ccaacctcac cgcggacatc catctgaagt tcctggcaga tggaccaagc caccttactt
     2101 ctggcggaat cctcgccgca tcagggatcg tcgaagatcg catgcccgag gtggaggccg
     2161 tcgcgcgcag cgtcgggttg cgttcactcg aagtcaagcg cgatgcggag tggcggtgtt
     2221 tggtactgac cagaagttga cgcaaccgtg caaacgcgca agcacgcaag cgcgatgact
     2281 gactaatgca tcgtttttca ctttcggaaa cgatctccgg cgaccgagtt gtgttgcggg
     2341 atgcgcaggc aaggcacgcc tctgtggtgc tgcgcgtgag gccgggtgag cggatcgtgg
     2401 tcttcgatgg gtccggccgg gagttcgacg tagaactcga gtcggtggat gcgagagcag
     2461 tgacaggccg catcgttgcc acgcggaaag ccggtagatt cccgctgcac atcaccttgc
     2521 tgcagggtgt tccgaagggc gcgaagatgg acgacgtcat ccgcatgggg acggagatcg
     2581 gggtcgcaga atttgtcccg ttcctatctg agcggacggt ggctgaagga cggcagcgag
     2641 cagatcggtg gcggagggtc gcgacagagg cggcaaaaca aagcgggcga agcgacgtgc
     2701 cggtcgtgca tgacccgtcg ccgctgaccc aagcgctcga ggggcttgcc gggcatgacc
     2761 tcctcctcgt gccgtgggaa gccgagcgcc accgatccat tcgagacgtg ctggagcaaa
     2821 gcgaccgggt gtcgaggatt gcgatcgcca tcggccctga gggcggactg gaagcccggg
     2881 agatcgaaga agccgcccgg tttggaggcg tggccgtgac gctcggcccc tcggtcctgc
     2941 ggaccgagac tgccgggatc gttgcggtgt ccatggtgct gtatggactg gtcatgagga
     3001 cgaccggcag gggagagtcg gctcccgcgt agaatagtcc gagcagtaaa gatagcgacc
     3061 attaacggta ccccgggagg gaccatggtt gcggctggga agactgacag tgctgagccc
     3121 tgtgcgtggg gcttaatttt tttgcatgtt tattcacgag ctggtatcat tatgcaacca
     3181 tgaccggacc gcgcgcatat ctcatcctag aagacggcgc catctttgaa ggggtccgta
     3241 tc
//
LOCUS       DBNGSB010000039         5666 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3401948, whole genome shotgun sequence.
ACCESSION   DBNGSB010000039 DBNGSB010000000
VERSION     DBNGSB010000039.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5666)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5666
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3401948"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="AC159B_01090"
     CDS             complement(<1..333)
                     /locus_tag="AC159B_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HIR3376382.1"
                     /translation="MHVAAGARIVEFGGWEMPVQYSGIVDEHRAVRAAAGLFDVSHMG
                     EVEVSGAGALESVQRLITNDAASLAVGQVLYSPMCTKSGGIIDDVTVFRLGEARYLFI
                     VNASTTAKD"
     gene            complement(365..1021)
                     /locus_tag="AC159B_01095"
     CDS             complement(365..1021)
                     /locus_tag="AC159B_01095"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009911333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HIR3376383.1"
                     /translation="MTFQDRREAGRQLAAALAVYPDRRPFVLAIPRGGVIVGYEVAKA
                     LGAPLDVVVPRKLRAPYNPELAVGAVAHDGSVYLDSPLVSHLDVTEEYVREETARQLE
                     EIRRRMQIYRGDRLPPALEGTASIVVDDGIATGSTMIAALRATRAARPSHLVAAIPVA
                     PAEGVAMLRREADDVVCLYTPPAFYAVGQFYVDFAQTDDAEVVELLRRREQEMNYGIS
                     "
     gene            complement(1100..1654)
                     /locus_tag="AC159B_01100"
     CDS             complement(1100..1654)
                     /locus_tag="AC159B_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HIR3376384.1"
                     /translation="MTRDEAWALVCEWITNPNLRKHLLAAEAAMRAYARKFEADEEAW
                     AAVGLLHDLDYERCPSQDAGHPFVGVEELRKRGVPETWPRAILSHADYSGVPRESLME
                     KTLYAVDELVGFIIAVALVKPGKTLAEVDVPSVRKKMKDKAFARGVNRDDVIKGADEL
                     GIPLDEHIDFVIGALQESAPQLGL"
     gene            complement(1651..2175)
                     /locus_tag="AC159B_01105"
     CDS             complement(1651..2175)
                     /locus_tag="AC159B_01105"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR3376385.1"
                     /translation="MASPRKRAPRKPRKNGKGSLHEWVLLHCRQTYQRRNWAGKGVLP
                     AIKGVTAEQAHWRPQPDQHNIAEIVLHMGYWKDRVTAGLTGRKWKYSDADNWRTVPHT
                     DAGWSAAQTELEAAQRRLMQGIRGLAPSRLMERAVGPWKWLDFIVDIATHDSYHAAQI
                     FVIRRLFSGRLTQT"
     gene            complement(2246..3862)
                     /locus_tag="AC159B_01110"
     CDS             complement(2246..3862)
                     /locus_tag="AC159B_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HIR3376386.1"
                     /translation="MSASVATAAERIDYASTLSGQTKAFILIGVLLGLFLAALDQTIV
                     ATALPAIIADLRGIDLLAWVSTGYLLASTAMVPIYGRLSDFYGRRPVLLFGILVFLAG
                     SALCGVAATMMQLVAFRVLQGIGAAALTSTAFAVPADLYPPAERARYQGLFGAVFALS
                     SVIGPYVGGLLTDQLNWRWVFYVNVPLGVVAIIFILARMPRLASGVKAAIDWAGTALL
                     ILAVVPLLLGLTLDKSLYPWSSPLIVGLFMMAAIATVGFIIVERRASAPVIPLDLFRN
                     RTFTVVVLASLLTGAAFFGAIVFISLFMVNVVGVSATAAGTTLMPLTMAVVAGAFTAS
                     MIVQRLGRYKVPMLVGYIISIIGFLLLSVMDASVTRSAVTWRMIVLGLGLGPTMPLLT
                     LVIQNAVSYDRVGAVTASRQFFMQIGAAIGIAVFGVVLSAALTSEFRSRVSPQLAQLP
                     PEVRGQIDLAQLRSGQGGRSAGLLARLGPRSPLRRATREAFAKSVTRVYRYAVFLAAA
                     ALLVTLALPELPLRTSNRPGAGPPVAAGGK"
     gene            complement(3906..4799)
                     /locus_tag="AC159B_01115"
     CDS             complement(3906..4799)
                     /locus_tag="AC159B_01115"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR3376387.1"
                     /translation="MQNLVEISDALAGIVEKAGQSVVRVEARRRWPSSGVVWADGLIV
                     TAAHVVERDDEIQVGIGGERTADATLVGRDPTTDVAVLRSDALTSTRIPQWADPGAVK
                     VGQLVLAISRPGRSARASLGIISAAGGSWRTPAGGEIERYLQPDVERALGFSGGLLVD
                     TSGAALGVNTSRLMRRTPMTIPAPTLRRVVETLIQHGRIRRGYLGIGGHPIRLPETIS
                     QQVGQRRGLIIISIEPGSPADRGGLFVGDIIVAIDQAPIRHPGEVSARLDEKSIGKPL
                     VVRVVRAGKTAEVTLTAGERQ"
     gene            complement(4873..5070)
                     /locus_tag="AC159B_01120"
                     /pseudo
     CDS             complement(4873..5070)
                     /locus_tag="AC159B_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015152194.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(5121..>5666)
                     /locus_tag="AC159B_01125"
     CDS             complement(5121..>5666)
                     /locus_tag="AC159B_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two pore domain potassium channel family
                     protein"
                     /protein_id="HIR3376388.1"
                     /translation="PSAGELLRRLGRWDDVGAVSAFLGRWEEWAADLLESHISYPILA
                     GFRSQHGNQSWVAGLTTILDACALVISSVEGVPQRAAHLTFAMARHVAVDLCQVLGSP
                     PEAPENDRLPRAELARLRTTLRDAGVSIREDQASAAALDDLRAMYEPYVNSLADFLLL
                     PLPNWLPVSGARDNWQKSKWV"
BASE COUNT         1098 a         1894 c         1711 g          963 t
ORIGIN      
        1 atccttggcg gtggtcgagg cgttgacgat aaagaggtag cgcgcttcgc ccagcctgaa
       61 tacggtcacg tcgtcaatga tgccacccga cttggtgcac atcggagagt acaggacctg
      121 accgaccgcc agagatgcgg catcgttcgt aatcaggcgc tgcacggact ccagcgcgcc
      181 ggctcccgag acctcgacct cacccatgtg cgacacgtcg aacagacctg ccgcggcacg
      241 aacagcgcga tgctcatcga cgatgccgga atattgaacc ggcatttccc agccgccgaa
      301 ctcgactatg cgcgcgccgg cggcaacgtg caattcaaat aagctcgtgc gcttgagttg
      361 gtggctaact gatcccgtag ttcatctcct gctcgcgacg cctgagtagc tcgacgacct
      421 ccgcgtcgtc cgtttgcgcg aaatcgacgt agaactggcc gaccgcgtag aatgcgggcg
      481 gggtgtacag gcagacgacg tcgtcggcct cgcggcgcag catcgccacg ccttcagcgg
      541 gagccaccgg gatggccgcg acgaggtgcg acggccgcgc cgcccgcgtc gcacggaggg
      601 ccgcgatcat cgtcgatcca gttgcgattc catcatcgac gacgatggat gccgtcccct
      661 cgagtgcggg cggcagtcgg tcgccgcggt agatctgcat ccgtcggcga atctcctcga
      721 gctggcgcgc ggtctcctcg cgcacgtact cctctgtgac gtccagatgg ctcaccaacg
      781 gggagtcgag atacacgctg ccgtcgtgcg cgaccgcgcc gacggcgagc tctggattat
      841 acggggcacg caacttgcgc gggaccacca cgtccagcgg agcgccgagc gccttcgcca
      901 cctcgtagcc cacgatgacg ccgccccgtg ggatggccag cacgaacggc cgccggtcgg
      961 gatacacggc gagcgccgca gccaactgtc gaccggcttc ccggcgatcc tgaaaggtca
     1021 tcgccgcgca gccccaatgt gagagagtcc ccgagcgtcc ggcgcgtcaa aggttccacg
     1081 acgcgcaaaa tttctcctat cacaggccca attgcggcgc cgactcttgg agtgctccaa
     1141 tcacgaaatc gatgtgctcg tccagcggta tgccgagttc atccgccccc ttgatcacat
     1201 cgtcgcggtt gacccctcgg gcgaatgcct tgtccttcat cttttttcga accgacggga
     1261 catccacttc ggccagcgtc ttgcctggtt tcaccagcgc cactgcaatg atgaagccga
     1321 caagttcgtc gacggcgtag agcgtcttct ccatcaacga ttcgcgcggc acaccggaat
     1381 aatcggcgtg cgacagaatc gcgcgcggcc aggtctcggg cacgccacgc ttgcgcagct
     1441 cctccacgcc gacgaacgga tggccggcgt cctgcgaagg acaccgctcg taatcaagat
     1501 cgtgcaggag gccgacggcc gcccatgcct cctcgtccgc ttcgaatttt cgcgcgtagg
     1561 cgcgcatcgc agcctccgcc gccagcagat gcttccgcag gttcgggttg gtgatccact
     1621 cgcaaacgag tgcccaggcc tcgtctcgag tcatgtctga gtaagccgac ccgagaacag
     1681 cctccgaatc acaaagatct gggcggcatg gtaactgtcg tgcgtggcga tgtccacgat
     1741 gaaatccagc cacttccacg gaccgacggc gcgctccata agacgcgacg gtgccaggcc
     1801 gcggatccct tgcatcagcc ggcgctgagc tgcctcgagt tcggtctgcg ctgcgctcca
     1861 tcccgcgtcc gtgtgcggca ccgtgcgcca gttgtccgca tcgctatact tccacttccg
     1921 gccggtgagc cccgcggtga cgcgatcctt ccaatagccc atgtggagaa cgatctcggc
     1981 gatgttgtgt tggtcgggct gcggccgcca gtgcgcctgt tcagcggtca cacctttgat
     2041 cgccggcagc acgcctttgc cggcccaatt ccgccgctgg taggtctggc ggcagtgcag
     2101 gagcacccac tcgtgcagcg agcctttgcc attcttgcgg ggtttccgcg gcgcgcgctt
     2161 ccgtggcgat gccacacgtc acctcactca catcagagtc ctcgccgact tccccggcat
     2221 cggaacggac tcctcgcgag tggagttact tgcctccagc cgccaccggg ggacccgccc
     2281 ccggacgatt gctcgtacgc agcggaagtt cgggcaacgc cagtgtcacc agcagcgcgg
     2341 cggcggcaag aaagaccgcg tatcgataga cgcgcgtcac gctctttgcg aaagcctcgc
     2401 gcgttgcgcg gcgcaatggc gagcgcgggc ccaagcgcgc cagcaggccg gcagaacgac
     2461 caccctggcc gctgcgcagt tgtgcgagat cgatctgtcc ccgcacctcc gggggcagct
     2521 gcgcgagctg ggggcttacg cgcgaacgga attcagaagt gagggccgct gacagcacca
     2581 cgccaaacac ggcgatgccg atcgcggccc cgatctgcat gaagaactgt cgcgaggcgg
     2641 tgaccgcgcc gactctgtca taggacaccg cgttctggat gaccagcgtc agcagcggca
     2701 tcgtcggccc aagaccaagg ccgaggacaa tcatccgcca cgtcacggcg gagcgggtta
     2761 cggacgcatc catcacggaa agcagcaaga acccaatgat ggagatgata tagccaacga
     2821 gcatgggcac cttgtagcgt cccagtcgct gcacgatcat cgatgcggtg aacgcccccg
     2881 ccaccacggc catggtgagc ggcatgagtg tcgtcccggc ggccgtggct gagacgccga
     2941 cgacgttcac catgaagagc gaaatgaaga ctatcgcccc gaagaacgcc gcgccggtca
     3001 ggagcgacgc gagcacaacc acggtaaacg tccggtttcg aaagaggtcg agggggatga
     3061 ccggcgctga tgcgcggcgc tcgacgatga tgaaccccac cgttgcaatc gccgccatca
     3121 tgaacaatcc gacgatcaga ggtgagctcc atgggtagag cgatttatct agcgtgagtc
     3181 cgagcaagag gggcacgacg gcgaggatta ataacgccgt gccggcccaa tcgatcgctg
     3241 ccttgacgcc acttgcgagc ctcggcatcc gcgccaggat gaaaatgatc gcgaccaccc
     3301 cgagcggcac gttgacgtag aacacccacc gccagttgag ttgatccgta aggagtccgc
     3361 cgacatatgg accgatgacg ctcgacagcg cgaacacggc gccaaacagt ccttggtagc
     3421 gtgcgcgctc cgcgggcggg tacagatcgg caggtacggc gaacgccgtg gaggtcagcg
     3481 ccgccgcacc gattccctgc aggacgcgaa aggcgaccag ctgcatcatg gtcgcggcga
     3541 caccacacag cgccgagcct gccagaaata caaggatgcc aaacagcagg acaggccgcc
     3601 ggccgtaaaa atctgaaagg cggccataga tgggaaccat cgcggtgctg gcgaggagat
     3661 atccggtcga cacccacgcc agcaggtcga tgccgcgcag atctgcgatg atcgccggta
     3721 acgccgtcgc gacgatggtc tgatcgagcg ctgccaggaa cagtcccagc aacacgccga
     3781 taagaatgaa cgccttggtc tggccagaga gggtggaggc gtaatctatg cgctcagcag
     3841 cagtcgcaac agatgcgctc accctcgatc tccccgacca ggttgcaggt ggctgtccat
     3901 tctccttatt gacgttcccc cgctgtgagg gtcacctcgg cagtcttccc ggcccggacg
     3961 acgcgcacga ccagaggttt tccaatcgac ttctcatcaa gccgcgcgga aacctcacca
     4021 ggatgccgga tcggcgcctg gtcgatggcg acaatgatat caccgacaaa caggccgccg
     4081 cgatcagccg gacttcccgg ctcgatggag atgataatga gaccccggcg ctgtccaacc
     4141 tgctggctga tcgtttccgg aaggcgaatc gggtgaccac cgattccaag atatccccgg
     4201 cgaatccgac cgtgttggat cagcgtttcg acgactctgc ggagtgtggg cgccggaata
     4261 gtcatcggcg tgcggcgcat caacctcgat gtgtttactc ccaaagcggc gcccgatgta
     4321 tcgaccagca acccacccga gaatcccaat gcgcgttcca cgtccggctg aagatagcgc
     4381 tcgatctctc cgcccgccgg cgtccgccag cttccaccgg ctgcgctgat gattccgagt
     4441 gaggccctgg cactccgccc cggcctcgag atcgcgagca cgagctgccc cactttgacc
     4501 gctcctggat cggcccattg cggaatccgc gtactggtca gtgcatcgct gcgcagcacg
     4561 gcgacatccg tcgtgggatc gcggccgacg agtgtggcgt cagctgttcg ttcgcctcct
     4621 atgccgacct ggatctcatc gtcccgttcg acgacgtggg cggccgtcac gatcaggcca
     4681 tccgcccaca cgaccccact cgagggccac ctgcgtcgtg cctcaaccct cacgaccgac
     4741 tgaccggcct tttcaacaat ccctgccagc gcatctgaaa tctcaactag attctgcatc
     4801 tataccacct ccggacccac gatgtcatgg atccgggccg cgggcatccc ccgttcggct
     4861 aggattgcag ggttagagaa tgatgagacc ctgacggacg gctctggtca ccgcctcagt
     4921 tctggagtgc gcttcaagct tgccaaaaat tgcggcaacg tggaacttcg cagtgtgctc
     4981 gctgatctcg aggcgttgag caattgcctt gttcgataac ccttcggcta gaagctgcag
     5041 cacctggtgt tctcgtgcgg tgagatcctc agcgggcagg atgtcttcgc gccggtcatc
     5101 gaggccgaca aaaacgaggt tcatacccat tttgacttct gccagttgtc ccgcgccccg
     5161 gatacaggca gccagtttgg caaaggcaac agcaggaaat ccgcaagaga gttgacgtac
     5221 ggctcgtaca tcgcgcgcaa atcgtcaagc gcagcggctg atgcctggtc ctcacggata
     5281 ctaaccccgg cgtcgcggag tgtcgtgcgc agccgcgcga gttctgcccg tggcaaccga
     5341 tcgttctccg gcgcctccgg cggggagccg agcacctggc acaggtccac agcgacgtgc
     5401 cgcgccatcg cgaacgtcag atgggcggcg cgctgcggca caccctcgac gctgctgatc
     5461 acgagggcgc atgcatccaa tatggtcgtc agccccgcga cccaggactg attcccatgc
     5521 tgcgatcgga aacccgccag gatcggatag gagatgtggc tttccagcag atccgccgcc
     5581 cattcctccc acctgccgag gaacgcgctt accgccccaa cgtcgtccca tctgcccaac
     5641 cggcggagga gttcccccgc cgatgg
//
LOCUS       DBNGSB010000040         4835 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3708674, whole genome shotgun sequence.
ACCESSION   DBNGSB010000040 DBNGSB010000000
VERSION     DBNGSB010000040.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4835)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3708674"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="AC159B_01130"
     CDS             <1..265
                     /locus_tag="AC159B_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIR3376389.1"
                     /translation="VPDFTTALIAALVLGIVNAFIRPIVLLLTLPINILTLGLFTLIV
                     NTLMLYIVAAVVQSFVITSFWSALIGALLIALISTVLSHVFTR"
     gene            267..1175
                     /gene="rapZ"
                     /locus_tag="AC159B_01135"
     CDS             267..1175
                     /gene="rapZ"
                     /locus_tag="AC159B_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HIR3376390.1"
                     /translation="MARPTAARRQSRLALSDIRFLIITGLSGAGKSTAMRVFEDLGFF
                     CVDNLPPALLPKFAELCLQSDGNIRQAALAIDVRGGEFFDDLFSALRHLEDLGFDYEI
                     VFLDSSDDVLVRRFEETRRKHPLSQTGGVLESIKEERQRLEAVKERADKIIDTSVLSV
                     KELRTELLDTYVRGRQEGPITVSVVSVGYKYGIPRDADLVFDVRFLPNPHYQEALRPL
                     PGHSEPVRRFVLEQPATREFMERLTEMMDYLLPQFITEGKAHLTVALGCTGGKHRSVV
                     LADELAEHLRQKGYNVSVRHRDVGKE"
     gene            1281..2615
                     /locus_tag="AC159B_01140"
     CDS             1281..2615
                     /locus_tag="AC159B_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006193562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HIR3376391.1"
                     /translation="MRDSFRRWAQWLVPGIGVKRWALVLAVSILLASAGVALIVNVRP
                     LGWLEVIVFQMALSFIVLTGGTLSPTALGVLLILVGLFVAFLGLRGTIRSIAGAFLPR
                     DDPRLVQVLHQQRQHRQRQRGPRIVVVGGGTGLSALLRGLKTFTDNLTAVVTVFDDGG
                     SSGRLRRELGILPPGDIRDCLVALAESEPLMTQLFEYRFQGGALDGHAFGNLFIASLA
                     GVTGDLESAVAATSKVLNIRGRVLPSAAEDVVLVAEFADGSVIEGESQIPRARKHIRR
                     MALKPTDVAALPDVLQAIREADLIVLGPGSLYTSVIPNLLVRGVGDAIRGSRALRVYV
                     CNVMTQPGETDGFAASDHVRALIDQIGPGLFTHVLVNTQRVRNRALLARYEAEGAQPV
                     EADFDAIRAAGVTPVPAHIVSEEDVVRHDSDRLGRVLMHLAAGAPAATVATL"
     gene            2821..3186
                     /locus_tag="AC159B_01145"
     CDS             2821..3186
                     /locus_tag="AC159B_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012411797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HIR3376392.1"
                     /translation="MPKVIVDWLYVAVFAFVGTIMAALPLGIVWLLAPRADLPQKTLT
                     YESGVIPFGQAWAQFNVRYYLFALIFVVFDVEVIYLYPWSVVFRQLGTFAFVEMVIFI
                     LILLVGLAYAWRKGALEWT"
     gene            3187..4353
                     /locus_tag="AC159B_01150"
     CDS             3187..4353
                     /locus_tag="AC159B_01150"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012373.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1/NuoH family protein"
                     /protein_id="HIR3376393.1"
                     /translation="MAWLAEILKAVLLTLLVFTFLAVVTAMLGVYLERKISGWIQARL
                     GPKHVGPQGLLQTVADTVKLLQKEHITPRQADVLIFNAAPVMVAVAGLLDWVVIPFGR
                     LGDRVLVVRDINIGVLYFAAMASLTVIGILAGGWASNNKYALLGGLRSASQMVSYEIP
                     LALGILWVAMIAGTLSTVGIVEAQRGLWFMFSVPPFGLLAAVIFLTAATAEVNRVPFD
                     LPEAESELVAGYFAEYTGMRFALFQLGEYAEAFAMAALAAIMFMGGWLEPNLPAWLWL
                     TLGIAGLLVAALLVVTGAARELLLRPILILALLGAVLSVIMFFGVQAGRSPVLPSPIW
                     FFTKMFALVFFLIWMRWTYPRLRLDQLLNLSWKVLLPAGLLNLLITGFLITVAR"
     gene            4354..>4835
                     /locus_tag="AC159B_01155"
     CDS             4354..>4835
                     /locus_tag="AC159B_01155"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012709.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J family
                     protein"
                     /protein_id="HIR3376394.1"
                     /translation="MGEFAAFIILSCVTLIPAVVVVTSGNIVRSALSLVPTFLGVTGL
                     YILLHAEFVAGIQILIYAGAITVLILFVIMLTAGGTGTRIRQVNEQVPLGAMAAVWLG
                     FLILLVLLRTRWPSAAATTPQYTVASVGTAFLTDYVLIFEVTSLVLLVSLIGAIIIAR
                     R"
BASE COUNT          747 a         1510 c         1516 g         1062 t
ORIGIN      
        1 ggtcccggac ttcacgacgg cgctcatcgc cgcactcgtc cttgggatcg tgaacgcttt
       61 catccgaccg atcgtgctgc tgctcacctt gccgatcaac atcctcaccc tcggtctgtt
      121 cacgctaatc gtgaatacct tgatgctcta cattgtcgcg gcggtggtgc agtcgttcgt
      181 gatcacgagt ttctggtcgg cactgatcgg cgcgctgctc attgcgctga tcagcacggt
      241 gctcagtcac gtgttcacgc gctgacatgg cgcgtccaac ggcagcgcgg cggcagagcc
      301 gcctcgcgct ctctgatatc cgcttcctca tcatcaccgg actgtccggg gcggggaaat
      361 ccaccgccat gcgcgtcttc gaggacctcg ggtttttctg cgtggacaac ctgccccccg
      421 cgctgctgcc gaagttcgcc gaactgtgcc tgcagtctga tgggaacatc cggcaggccg
      481 ccctcgcgat cgacgtacgc ggcggggagt ttttcgacga tttgttcagc gccctgcgcc
      541 atctggagga cttgggattc gactacgaga tcgtcttcct tgattcgtcc gatgacgtac
      601 tggtccgccg gttcgaggag acgcgccgca agcacccgct gtcacaaacg ggcggcgtgc
      661 tcgagagtat caaggaggag cgccagcgac tggaagcggt gaaagagcgg gccgacaaga
      721 tcatcgacac ctcggtgttg tcggtcaaag agcttcgcac ggagctgctc gatacctacg
      781 ttcgcgggag gcaggagggg ccgattaccg tgagcgtcgt ctccgtcggc tacaaatacg
      841 ggattccccg ggacgcggat ctcgtcttcg acgttcggtt ccttcccaac ccccactatc
      901 aggaagcgct ccggccgttg ccggggcaca gcgagccggt gcgccgtttc gtgctcgagc
      961 agccggccac tcgcgagttc atggaacggc tgaccgagat gatggactat ctgctgccgc
     1021 agtttatcac tgagggcaag gcgcatctga ccgtggcact cgggtgtacg ggtggaaagc
     1081 atcgctcggt cgtgcttgcc gacgagctcg cggagcacct gcggcagaag gggtacaacg
     1141 tcagcgtgcg gcaccgcgac gtaggaaagg agtaagcggc atggcggcgg cgcaaagcct
     1201 ctccccgact gaaactaata cggggacccc tctggctttg cacctccgcc tgccgctagt
     1261 agaagtggca agtaatgaag atgagggata gttttcggcg ctgggcgcag tggttggtcc
     1321 ccggcatcgg ggtcaagcgc tgggcgcttg tgctcgcggt cagcatcctg ctggcgagcg
     1381 ccggcgtcgc gctcatcgtc aacgtcagac cgcttggatg gctggaagtc atcgtcttcc
     1441 agatggcact gtcattcatc gtcctgaccg gcggcacgtt gtcgccgacc gcgctgggcg
     1501 tgctgctgat cctcgttgga ctgttcgtcg ccttcctggg gcttcgcggc acgatccggt
     1561 cgatcgccgg cgcgtttctt cccagagatg atccacgtct cgtccaggtc ttacatcagc
     1621 agcgtcagca tcggcagcgc cagcgtggcc cacggatcgt ggttgttggg ggcgggaccg
     1681 gtctgtcggc tctgctccgc ggcctcaaga cttttactga caacctcacc gcggtggtga
     1741 cggtgtttga cgacggcggg agctccggcc ggctgcgccg ggagctcggc atccttccgc
     1801 ccggggacat ccgtgactgt cttgtcgcgc tcgccgaatc cgagccgctg atgacgcagc
     1861 tcttcgagta tcgctttcag gggggcgcgc tcgatgggca tgccttcggc aatctcttca
     1921 tcgccagcct ggccggcgtt accggtgacc tggagagcgc ggtggcagcg acgagcaaag
     1981 tcctcaacat ccgcggccgg gtcttgccct cagcggccga ggacgtggtg ctcgtcgcgg
     2041 aatttgccga cggcagcgtc atcgaaggcg agtctcaaat cccacgggcc cgtaagcaca
     2101 tccgcaggat ggccctcaag cccaccgacg tcgcggcgct gccggacgta ttgcaggcta
     2161 tccgcgaagc ggacttgatc gtgctgggcc cgggcagcct gtataccagc gtgatcccga
     2221 accttcttgt ccggggcgtg ggcgacgcca tccgcggcag ccgcgcgctt cgcgtgtacg
     2281 tctgcaatgt catgacccag ccaggcgaga ccgacggttt tgccgccagc gatcacgtac
     2341 gtgcattgat cgatcagatc ggcccgggac tcttcacgca tgttttggtt aacacccaac
     2401 gggtccgcaa ccgcgcgctc ctggcgcggt atgaggctga gggcgcgcag cctgtcgagg
     2461 cggatttcga tgccatccgc gccgcgggtg tcacccctgt ccccgcgcac attgtgagcg
     2521 aggaagacgt ggtgcgccat gattccgacc gcctgggccg cgtgctgatg cacctcgccg
     2581 ccggcgcgcc tgccgctacc gtagcaactc tctaacagac gacaatcgga caactggaca
     2641 acagggataa caattgcgtc atcgcccacg ttgtccccgt catcctgttg tcccgtgttc
     2701 gcgtgcggca ggtttcatca gcaagtcact cgaatttcga agaattggtt tgtgtccaac
     2761 cctcttcgtt ttcgtctgtt ggtcgtggat ccatgagttc tgggcgaggg cctgcgcata
     2821 atgccgaaag ttatcgttga ctggctgtac gttgcggtgt tcgcgttcgt cggcacgatt
     2881 atggccgccc tgccactggg gattgtctgg ttgctggccc cccgcgccga cctgcctcag
     2941 aaaactctga catacgagtc cggcgtgatt ccttttggcc aggcatgggc acagttcaac
     3001 gtccgctact atttgttcgc gttgattttc gtcgtctttg acgtggaagt catctacctc
     3061 tacccgtggt cggttgtgtt ccgtcagctg ggtaccttcg cgttcgttga gatggtcatc
     3121 ttcatcttaa tactgctcgt ggggctcgcc tacgcgtggc gcaagggcgc cctcgaatgg
     3181 acgtagatgg cgtggctggc tgaaattctc aaggcggtcc tcctcacgct gctggtgttc
     3241 accttcctgg cggtcgtcac agccatgctc ggcgtctacc tcgagcgaaa aatcagcggg
     3301 tggattcagg cgcgtcttgg tcccaagcat gtgggtccgc aagggctgct gcaaacggtc
     3361 gctgataccg tcaagctcct gcagaaggag cacatcacgc cgcgacaggc cgacgtcctg
     3421 atcttcaatg ccgcgccggt tatggtggcg gtcgccgggt tactcgactg ggtggtaata
     3481 ccgttcgggc ggctggggga ccgcgtgctg gtcgtccgtg acatcaacat cggcgtcctg
     3541 tactttgccg cgatggcatc tttgaccgtc atcggcattc tcgcgggcgg ctgggcatca
     3601 aacaacaagt acgcgctcct gggcggtctg cgctccgcct cacagatggt gagctacgag
     3661 attccccttg ccttagggat cttgtgggtc gcgatgatcg ccgggacgct gtccaccgtc
     3721 gggatcgtcg aggcgcagcg aggcctgtgg ttcatgttca gcgtgccccc cttcggcctt
     3781 ctcgccgcgg tgatatttct gacggcggcc acggcggaag tgaaccgtgt tccgttcgac
     3841 ctccccgagg cagaatcgga actggtggcg ggctatttcg ccgagtacac cggcatgcgt
     3901 tttgcgctct ttcagctggg ggaatatgct gaagcgttcg cgatggccgc gctggccgcg
     3961 atcatgttca tgggcggatg gttggaaccc aacctgcccg cgtggctctg gttgacgctg
     4021 gggatcgccg gcctgctcgt ggccgcccta ctcgtcgtga ccggggccgc gcgcgaattg
     4081 ctcttgcggc cgatcctcat ccttgcgctg cttggtgccg tgctctccgt gatcatgttc
     4141 ttcggcgtgc aggccggccg ctcgccggtg ctgccttcgc ccatctggtt tttcacgaag
     4201 atgttcgcgc tggtcttctt cctgatctgg atgcgctgga cgtatccgcg tctgcggctc
     4261 gatcaactgc tcaacctgtc gtggaaggtg ctgttgcccg cgggactgct gaatctcctc
     4321 atcacggggt tcctcatcac ggtcgctcga taaatgggtg agttcgccgc gttcatcatt
     4381 ctgtcgtgcg tcaccctgat ccccgcggtc gtggtagtga cctcagggaa cattgtgcgc
     4441 agcgcgctat ctctggtccc aacgttcctg ggcgtcaccg gcctctacat cctgctgcat
     4501 gcggagtttg tcgcgggcat ccagatcctc atctacgccg gagccattac cgtgttgatc
     4561 ctatttgtca tcatgttgac cgcggggggc accgggacgc gcatccggca agtcaatgaa
     4621 caggtgccgc tcggcgccat ggcggcggtc tggctcgggt tcctcatcct gctcgtcctc
     4681 ctccgcaccc gctggccgtc agcggccgcg accaccccgc aatacaccgt ggcctcagtg
     4741 ggcacggcgt tcctgacgga ctacgtcctg atcttcgagg tcacgagcct cgtgctgttg
     4801 gtctcgctca tcggggcgat cattattgcg aggcg
//
LOCUS       DBNGSB010000041         7285 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3862024, whole genome shotgun sequence.
ACCESSION   DBNGSB010000041 DBNGSB010000000
VERSION     DBNGSB010000041.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 7285)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7285)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7285
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3862024"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /gene="miaA"
                     /locus_tag="AC159B_01160"
     CDS             complement(<1..592)
                     /gene="miaA"
                     /locus_tag="AC159B_01160"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HIR3376395.1"
                     /translation="MVSSQVAIVQKPAPEELARQEGRPLIVICGPTATGKTAAAVELA
                     ERIGGEIIAADSRTVYKEMDIGTAKPTPALQARVRHHLIDIAPPDEVMTVATYRRLAL
                     AAMGEIQLRGREVLLVGGTGLYIRAITDGLTIPEVAPDWSLRERLEGLERDAPGTLYA
                     RLQAVDPVSASRIHPKNIRRLIRALEVYEHTRRPISE"
     gene            complement(574..2262)
                     /gene="mutL"
                     /locus_tag="AC159B_01165"
     CDS             complement(574..2262)
                     /gene="mutL"
                     /locus_tag="AC159B_01165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HIR3376396.1"
                     /translation="MGNIRILEKSVAERIAAGEVVERPSSVVKELVENSLDAGATQIV
                     IEVEGAGSTMIRVGDNGSGMEPADLRLAIRRFATSKITSLDDLETAASFGFRGEALPS
                     IAAVSHLEISSAAVGSAAGSRLRSVGGEESTDDMVGIPPGTTVSVRQLFFNTPARRKF
                     LKSPSREFALIVDALNRLALAHPHVAFRLVHEGEEVLRYPAGRDEDRIAAVLGGAVFD
                     RMLPLNEGVADIAISGWTGKPDLARTVRRHQYVYINRRSVQSRVIGGAVEQSYQQLVP
                     AGRFPIFVLFVNLPGRRVDVNVHPRKSEVRFDDEHQVFAAVSRVVRGALRSAPIAPAV
                     GKFSPSPMEAGAPLMLGDLRGFMPVAEPLEVSSAGRLPAMRLLGQLHGTYLLAQSTDG
                     LVVIDQHAAHERVLYERLIRERANPTGNGEVLIDPAPVSLSVGEAVMLAEHLEILEQI
                     GFDLEPFGDRTVLVRGVPGITRGHSPETLLRDLLAELSESPRATAAQALIERAAITTA
                     CHTAIRAGDRLGSEQMMALLRDLSETADPFTCFHGRPTLATISLPAIERWFLRR"
     gene            complement(2286..4970)
                     /gene="mutS"
                     /locus_tag="AC159B_01170"
     CDS             complement(2286..4970)
                     /gene="mutS"
                     /locus_tag="AC159B_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HIR3376397.1"
                     /translation="MDSDLTPMMQQYHQLKARFPGMLLMFRLGDFYELFYDDAQVASR
                     ELEITLTSRETGKGRRVPMCGVPYHALNTYLSRLVERGYRVAVCDQLEDPRRAKGLVK
                     RDVVRIVTPGTVVEHAMLPADANNYLMAVAPKAGAWGLAVADLSTGEFAVTELRGEQR
                     DAQLTEELARWSPAEVLIPETSTTDLKAHLGGARITTVDEWRFDPAIARGLLLEHFGV
                     ATLDGFGCEDLPMAVAAAGALLHYLKDTQFSPLAHMHRLVTYSPEELLALDSATSRNL
                     ELVANLRDGGTAGTLFEVLNDTKSRMGARKLRHWLLQPLVDPQQIVARHDGVEYFVGH
                     ARDQEAIHEALHRMPDLERLTGRVGHGSADARDLVAVAAALDRIPEIQTILQRTGDQH
                     LLQLAVSIDPHDEVKTLVTRAIVESPPLSVHEGGVIRDGYNEELDQLRAGAREGKAWI
                     ASLETQERARTRIRSLKVGFNRVFGYYLEVSKANLHLVPADYVRKQTLAGVERYVTEA
                     MKEREAAILGAEERIAELEYALFSDVRDRVAQSSDTLLLTAAALAETDVLASLASVAA
                     RWEYVRPVITGDLVLEVTAGRHPVVERLRESERFVPNDIALSAEDRTILVVTGPNMAG
                     KSTYLRQAALITLMAQMGSFVPAAAATVGIADRIFTRVGAIDDIATGRSTFLVEMQEV
                     ANILHHATKRSLVILDEVGRGTSTYDGMSLAWAVVDYLHDRIGPRTLFATHYHELTEL
                     AALLPRVRNVNVLVKEEGDRVVFIRKVVDGGADRSYGIHVARLAGLPPAVIEHAQRIL
                     RQLEATSSSVRSVEEGFLPPIPSRATGALQLPLPLAPISLLEEQILGLSLESLTPLEA
                     LNALHTLREQVRQRVEETRSAALPGKIVRMKRHRKNPN"
     gene            5223..6296
                     /locus_tag="AC159B_01175"
     CDS             5223..6296
                     /locus_tag="AC159B_01175"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR3376398.1"
                     /translation="MHAIEKERSKKQCVLLATSGLLLALVVTYALNFLGLETLTTAQF
                     ALAAALTVSAQVALWLVVHFGLDARLTWDRHYLYVPMMVAIVLLSLYVYLAPPVRMLM
                     LMAWFSSLVFMAGLIGLAGVVMLALMMAVGYLTAVATLINRGSAIYLPLEVTAAATFL
                     VINVYAGVVFERLRREHDETRALRTNLAELAITDPLTGLHNRRHFEEILRDEVARVKR
                     YGGFCALAMLDLDFFKSYNDTLGHLAGDDLLRDLAQMLRRHIRVTDVLARYGGEEFGL
                     IMVNTPKEVAIAAMERLRGLIETYPFRGGNILPDGRLTVSVGIAGCPEDSVDFEELVR
                     RADAALYIAKRRGRNQVQAAAIA"
     gene            6405..6554
                     /locus_tag="AC159B_01180"
     CDS             6405..6554
                     /locus_tag="AC159B_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376399.1"
                     /translation="MIVYVEAFAPQSLLQRVEGGLQLILTKWVTGVILVAATLQMLSD
                     LGLIP"
     gene            6683..7237
                     /locus_tag="AC159B_01185"
     CDS             6683..7237
                     /locus_tag="AC159B_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376400.1"
                     /translation="MKYLAGPAQAAIVTALLTLLAFTPAAGQTPAQRGNSFSVLISST
                     AGATAFRVGMTFRVNPTLDAVFAYSTVPSFAASELDAGFRYHFPVRSSVGFDPYIGAG
                     LAIAQIGGLGVTGVFLQTGAAVTLAPQLRGFGGVSYVAAGGTTALVYDIGAEYLFSQQ
                     VRGVVGVTGASGSSSLYFGATLNY"
BASE COUNT         1328 a         2294 c         2263 g         1400 t
ORIGIN      
        1 attccgagat tgggcgcctt gtgtgctcat agacctcgag cgccctgatg aggcgccgga
       61 tattcttggg atggattcgt gacgccgaca caggatcgac ggcctgcaat cgtgcataca
      121 gcgtgccggg cgcgtcgcgc tcgagcccct cgaggcgctc gcgcagcgac cagtcgggcg
      181 ccacctcagg gatggtgagt ccatccgtga tcgctcgaat atatagcccg gtccctccca
      241 caagcagcac ctccctgccc cgtagttgta tctcacccat cgcagccagg gccaaccggc
      301 ggtaggttgc gacggtcatg acttcgtcgg gcggcgcgat gtcgatgagg tgatgtcgca
      361 cgcgcgcctg gagggccggg gtcggcttgg ccgtcccgat atccatctct ttgtagacgg
      421 tgcgagaatc ggcggcaatg atctcgccgc ctatgcgctc ggcaagctcg acggccgcgg
      481 cagtcttgcc ggttgccgtg ggcccgcaga tcacaatgag gggccgtcct tcttgccgcg
      541 ccagctcctc gggggcgggc ttttggacaa tcgctacctg cgaagaaacc atcgctcaat
      601 tgcgggaagc gagatcgtgg caagtgtagg ccgcccatgg aaacaggtga atggatctgc
      661 ggtctctgag aggtcgcgca acaaagccat catctgctcc gaccccagac gatcccctgc
      721 cctgatcgcc gtatgacacg ccgtagtgat ggcggcacgc tcgatcagcg cctgcgcggc
      781 cgtggcacgc gggctctcgg agagttccgc gagcagatcc cgcagcaggg tctccgggga
      841 gtgtccgcga gtgataccag gcactccgcg caccagcacc gtccgatccc caaaaggttc
      901 gaggtcgaat ccgatctgtt cgaggatctc gaggtgctcg gccagcatga ccgcctcacc
      961 taccgatagc gagaccggtg ccggatcaat tagcacctct ccgttgcctg ttggatttgc
     1021 gcgctcacga atgagacgct cgtagaggac ccgctcatgg gcggcgtgct gatcgatgac
     1081 gacgagacca tcggtactct gggccaggag ataggtccca tgcagttgtc ccaacaacct
     1141 catcgcgggg aggcggccgg cggagctcac ctccagtggt tccgcgaccg gcatgaaacc
     1201 acggaggtct cccagcatca gaggtgcgcc ggcctccatc ggagatggcg aaaatttgcc
     1261 aaccgctggc gcaatcggag ccgatcgcag cgcgccccgt accaccctcg ataccgctgc
     1321 gaaaacctga tgctcgtcat cgaaccggac ttccgatttg cgcggatgga cgttcacgtc
     1381 gacgcgccgc cctggaaggt tcacaaacag cacgaaaatg gggaaccggc cggcgggaac
     1441 gagttgttga taggattgct cgaccgcgcc accgattact cggctctgca cgctccgccg
     1501 gttgatgtac acgtattggt gccgccggac cgtccgcgca agatccggtt tccctgtcca
     1561 gcccgagatc gcaatgtcgg cgacaccctc attcaacggc agcatccggt caaacactgc
     1621 gccgccgaga accgccgcga tacgatcctc gtcgcgtccg gccgggtacc gcaggacctc
     1681 ttcaccctcg tggacgagcc tgaaggccac atgcgggtgg gccagcgcga gacggttgag
     1741 ggcatcaaca atcagcgcga attcgcgaga tggtgacttc agaaattttc gcctggccgg
     1801 tgtattgaag aagagctgcc ggacagacac ggtcgtcccg ggcgggatcc cgaccatgtc
     1861 gtcagtcgac tcttccccgc ctaccgaccg taaacggctt cccgcagcag atcccaccgc
     1921 tgccgaggag atctccaaat gtgagacggc ggcgatactg ggcagcgctt cgccccgaaa
     1981 cccaaacgac gctgcggtct ccagatcatc gagtgaggtg atcttgctgg tggcgaacct
     2041 ccggatagcc aaccgcagat cggcgggctc catcccgctc ccgttgtcgc cgacgcggat
     2101 catcgttgag cccgccccct cgacctcgat gacgatctgc gtggcgcctg cgtcgagact
     2161 gttctcgacg agctccttca cgaccgagga aggacgctcg accacctcgc ccgcggcaat
     2221 tcgctccgcg acactctttt caagaattcg aatgttgccc atggttgagt cgctacgcgt
     2281 ttgcgtcagt tagggttctt gcggtgtcgt ttcatccgca cgatctttcc cggcagcgca
     2341 gcgcttctcg tttcctcaac ccgctggcgc acttgttcgc gcagtgtatg cagcgcattg
     2401 agggcttcga ggggggtcag cgactccagg ctcaacccaa gtatctgctc ttcgagcagt
     2461 gagatcggcg ccagcggcag cggcaattgc agcgcacccg tcgcccgaga cggaatcggc
     2521 ggcaagaacc cttcctcgac tgaacggacc gaggaggatg tggcctccag ctgcctgagg
     2581 atgcgctgcg cgtgctctat caccgcaggt ggaagcccgg cgagcctggc gacgtggatg
     2641 ccgtaactgc ggtcggcgcc tccgtcgacg accttccgga tgaacacgac acgatctccc
     2701 tcttccttca ccagcacatt gacgttgcgg accctcggca gcagcgcagc cagttccgtg
     2761 agctcgtgat agtgagtcgc gaacaatgtg cgcgggccga tccggtcgtg cagatagtcc
     2821 acaacggccc aggcgaggct catcccatcg taggtgctgg tccctcgccc gacttcgtcc
     2881 aagatgacca aactccgctt ggtggcgtgg tgcaggatgt tcgcgacctc ctgcatttcc
     2941 accaagaacg tgctgcgccc agtcgcaatg tcgtcgatgg cgccaacccg cgtgaagatg
     3001 cgatcggcga tcccgaccgt agccgctgcg gccgggacga acgaccccat ctgcgccatg
     3061 agcgtaatca gggccgcctg ccgcaggtag gtcgacttcc cggccatgtt cggtccggtg
     3121 acgacaagga tggtccgatc ttccgctgag agcgcgatgt cgttcggcac gaatcgctcg
     3181 ctctcacgca accgctccac gacgggatgg cgtccggcgg tcacttcaag caccaggtcg
     3241 cccgtaatca caggacgcac atactcccac cgtgcggcga ccgatgccag agaggccagc
     3301 acgtcggttt cagccagcgc cgccgcggtc aagagcagtg tgtccgacga ctgggcgacg
     3361 cggtcgcgca cgtcggaaaa caacgcgtac tcgagctccg cgatccgttc ttcggcgccg
     3421 agaatcgcgg cttcgcgctc cttcatggcc tcagtgacgt atcgctcgac gcccgcgagc
     3481 gtctgctttc tgacatagtc cgccgggacc aaatgaaggt tcgccttgct gacctcgagg
     3541 tagtagccga agactcgatt gaacccgacc ttcagggatc ggatccgggt gcgcgcccgc
     3601 tcctgggtct ccagactcgc gatccaggct ttcccctcac gggcgccggc ccgcaactga
     3661 tcgagctctt cattgtatcc gtcccggatc accccgccct catgcacaga caacggtggt
     3721 gattccacga tcgccctcgt gacgagtgtt ttgacctcat catgcgggtc gatgctcact
     3781 gccagctgca gaagatgttg atcgcctgtt cgttgaagaa tcgtctggat ctccgggatc
     3841 ctgtcgagcg cggcggccac ggcgacgagg tcacgggcat cggctgagcc gtgtcccacc
     3901 cgtccggtca ggcgctccaa atcaggcatg cggtggagcg cctcgtggat tgcctcttga
     3961 tcgcgcgcgt gcccgacaaa gtactccacc ccatcgtgcc gtgccacgat ctgctgcgga
     4021 tcaactaaag gctgcagcag ccagtgccgg agcttgcggg ctcccatccg actcttcgtg
     4081 tcgttgagga cttcgaagag tgtgccggcc gtaccgccgt cacggagatt tgcgaccagt
     4141 tcgaggttcc gagaggtcgc gctgtcaagc gccagcagct cctctgggct atacgtgacg
     4201 agccggtgca tgtgcgccag ggggctgaac tgcgtgtcct tcaggtaatg cagcagcgcg
     4261 ccggcggccg ccaccgccat cggaagatcc tcacagccga agccgtccag ggtggcgacc
     4321 ccgaagtgct ccaacagcag cccgcgtgcg atcgcaggat cgaaccgcca ctcgtcgacc
     4381 gttgtgatgc gcgcgccgcc gaggtgcgcc ttgaggtccg tcgtgctcgt ctcaggaatg
     4441 aggacctcgg ccggactcca gcgggccagt tcttcggtga gttgcgcgtc tcgctgctca
     4501 cctcgcagct cggtgacggc aaactcaccc gtcgagaggt cggcgacggc gagtccccag
     4561 gcgcccgcct taggcgcgac agccatgagg tagttgttcg catcggcggg aagcatcgcg
     4621 tgttctacaa ccgtccctgg agtgacgatg cgcacgacat cgcgcttgac cagtcccttc
     4681 gcgcgcctgg gatcctcgag ctggtcgcag accgccacgc ggtagccgcg ctcaaccagc
     4741 ctggagagat aggtgtttag cgcgtggtac gggacaccgc acattgggac gcggcgcccc
     4801 ttgcccgttt cgcgcgaggt cagggtgatt tccagctcgc gtgatgcgac ctgcgcatca
     4861 tcgtaaaaca gctcgtagaa atcgccgagc cggaacatca gcagcatccc aggaaagcgg
     4921 gccttcaact ggtgatattg ctgcatcatc ggcgtgaggt cagagtccat ctaagcgcac
     4981 caacggagct ctccgggtaa gccaattctt cggcgggcac ctaccctctc ccgccactgg
     5041 gagatgatgg atcgcgcgag acgacagcag gagggccgtc gcatccgtac aatgcttttt
     5101 cggatttgtc gcgcctcggg aaactatggt ttgtcgagtg ccatcaaacc cgcctcaaac
     5161 caacgggatg ccggagaagc gggcggtctc gttcttgcta tctaggttag atggatacac
     5221 ctatgcatgc gattgaaaag gaacggagca agaaacagtg tgtcctgctg gccacgagcg
     5281 ggctgctcct ggcgctggtg gtgacgtatg ccctcaattt cctagggctc gagacactga
     5341 ctactgcgca gttcgcgctg gccgcggcgc tcactgtgag cgcgcaggtg gcgctgtggc
     5401 tggtcgtcca cttcggattg gatgcacgcc tgacttggga ccggcattat ctgtatgtgc
     5461 cgatgatggt tgcgatcgtg ctcctgagcc tgtacgtata cctcgctccg ccggtgcgga
     5521 tgctcatgct catggcatgg ttcagctctc ttgtgtttat ggccgggctg atcggtcttg
     5581 cgggtgtcgt catgttggcg ctgatgatgg ccgttgggta tctcaccgcc gtggcaacct
     5641 tgatcaatcg tggatccgcc atctacttgc cgttggaagt cacagcggcc gccacgtttc
     5701 tggtgatcaa cgtgtatgca ggcgtggtgt tcgagcgcct gcggcgcgag cacgacgaaa
     5761 cgcgcgcgct gcgaacgaat ctcgccgagc tggcgatcac cgatccactg acggggttgc
     5821 acaaccgtcg gcacttcgag gagatcctgc gggacgaggt cgcgcgtgtc aaacgttatg
     5881 gcggattttg cgcgctggcg atgctggacc tcgatttttt caagagttac aacgatacac
     5941 tcgggcacct ggccggcgac gatctcctcc gcgacctggc gcagatgctg cgcaggcaca
     6001 tacgcgtgac cgacgtgctg gcccgctacg gcggcgagga gtttgggctg atcatggtca
     6061 acacacccaa agaggtggcc atcgccgcga tggagcggtt gcgcggactc atcgagacgt
     6121 atcccttcag gggcggcaac atactgccgg acggccgctt gaccgtgagc gtgggcatcg
     6181 ccgggtgtcc agaagacagc gtggacttcg aagaactcgt tcgccgcgcg gacgcagctc
     6241 tctacatcgc caaacgccgc ggccggaacc aagttcaggc ggccgcgatc gcgtagttga
     6301 attttcctcg ttgcgactaa gagaagttta atggaagttt tttcgaaccc cgtggcacag
     6361 cccatgctgt gctgtaactc aggtcgaacg cgcgggagga agcgatgatc gtctacgtcg
     6421 aggccttcgc accacagagt ctgctgcaga gagtcgaggg cggtctgcag ctgatcctaa
     6481 ccaagtgggt gacgggcgtc atcctggtgg ccgcgacgct gcagatgctc tcggatctcg
     6541 gactcatacc ctaaagaaac ttcgcggacg gcgggcggac aacgaagggc cgggtcggtg
     6601 aacccggccc ttcaaatttg cgagagtcta cgagacctgc gaacagggaa cgacccgaga
     6661 gtcagaataa agggggcaga cgatgaagta tttggctgga ccggcgcaag ccgcgattgt
     6721 aacagcgttg cttaccctgc tggccttcac tccggcagcc gggcagacgc cggcgcagcg
     6781 cgggaactcc ttcagcgtgc tgatctcctc cactgcgggt gcgacagcct tccgggtggg
     6841 aatgacgttc cgagtgaacc ctacactaga tgccgtgttt gcatatagca ccgtgccatc
     6901 gtttgccgcc agtgaattgg acgcgggctt tcgttaccac ttccctgtac ggtcgtcggt
     6961 gggcttcgat ccctacatcg gcgccggtct tgctatcgct caaattggag gcctcggggt
     7021 caccggtgtg ttcttgcaaa cgggggccgc ggtaacgctc gcaccgcagc tgcgcggctt
     7081 cggtggtgtg agttacgtcg cagccggcgg aacaactgcg cttgtctatg acatcggagc
     7141 cgaatatttg ttttcacagc aggtcagggg cgtcgtaggg gttacgggcg caagcggctc
     7201 ctccagcctt tacttcggcg ctacgctcaa ctactaagga gatagcctct gcattcctgg
     7261 ctgcggcgac actggcgtgt cgccg
//
LOCUS       DBNGSB010000042        10849 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_4168862, whole genome shotgun sequence.
ACCESSION   DBNGSB010000042 DBNGSB010000000
VERSION     DBNGSB010000042.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 10849)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10849)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10849
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_4168862"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..1053
                     /gene="efeU"
                     /locus_tag="AC159B_01190"
     CDS             223..1053
                     /gene="efeU"
                     /locus_tag="AC159B_01190"
                     /inference="COORDINATES: protein motif:HMM:NF041756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HIR3376401.1"
                     /translation="MAGSFLITLREGLEAALIVGIILAYLAKTGNRGKSGVVWMGVGL
                     SVLMSVLVGAAIFLTAGGLAGRSEARFEGVAMFTAAGVLTYMIFWMRKQAINIRAHLQ
                     RQLSTALESGSLTALGLLAFVAVGREGIETALFMFAATKAATPAAATLGGLLGLVAAV
                     ALGYVLYRGTYRLNLRAFFNVTSVLLLLFAAGLLAHGIHEFHEAAVIPPVVDELWNTN
                     HILDEQSTVGSFARALFGYNGNPSLVEALAYLTYLLLVGWMYFRPPTAKELTPGRASA
                     "
     gene            complement(1054..2433)
                     /locus_tag="AC159B_01195"
     CDS             complement(1054..2433)
                     /locus_tag="AC159B_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HIR3376402.1"
                     /translation="MRPQITPTIFREYDIRGVVGREITVDTAGAIARAYASYLSERGE
                     RKVVVGRDLRESSPEIRDAAVAALIDSGCDIVDIGTVITPVFYFARVHLGIDGGLMIT
                     ASHNPPEFNGFKLAHGFGTIYGEEIQEIRRIAGSGEFVSGRGRVEKHDVLQAYRTMLK
                     EKIRLGSRRLRAVVDCGNGTASLIAPQALEDLGVEVIRLYCDPDPKFPNHHPDPVDPD
                     NLVDLIATVRREKADVGVGFDGDGDRIGVVDDQGTILWGDMLMVVFWREILPKYPGTP
                     GIVEVKCSQVLYDEIARLGGKPMFYRTGHSLIKAKMKEIGAVFTGEMSGHMFFADEYY
                     GYDDAVYAAARLLRILSNSPRPLSALLADVPRYPVTPEVRVDCPDEGKFEVVRRLTDE
                     FRATYEVIDIDGARVLFPDGWGLIRASNTQPALVLRAEGKTPEALAEIKRTLEAALSK
                     FPAVGPIDW"
     gene            2480..3181
                     /gene="lipB"
                     /locus_tag="AC159B_01200"
     CDS             2480..3181
                     /gene="lipB"
                     /locus_tag="AC159B_01200"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HIR3376403.1"
                     /translation="MRPGWLLNVTDRVTPYAHAWDLQKQLVEARQHGRLGDGLVLVQH
                     EPVFTLGRSSRSEHLPFPRRALAQLGFGVFEIERGGSVTYHGPGQLVGYPILDLRSYG
                     DDVVQFVRSLEESVLGTLADFDIEAQRIRGFPGVWAGNEKIAAVGVAVKRKVTMHGFA
                     LNVDPDLSHFAYINPCGLNKPVTSVAKILGRPVSLAEVGLSYARRFSEVYGLTMSSML
                     PEALLSAASESPVPV"
     gene            3189..4334
                     /locus_tag="AC159B_01205"
     CDS             3189..4334
                     /locus_tag="AC159B_01205"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HIR3376404.1"
                     /translation="MTRRRFETRAIHDGQDPDPTTGSVIPPIYQTSTFQQSAPAEHKG
                     YEYARTGNPTRTALEACLASLEDGQFGLAFASGMAAITTTAYLLSPGDHAVLPDDVYG
                     GTYRLFVRVLERYGIASSFVDMTDLTRVEAALTPKTRMVLTETPTNPYLKILDIAAIS
                     ELAHARDALVVVDNTFASPYFQRPLSLGADLVVHSTTKYLGGHSDVIGGAVVVNSNDI
                     YQTLKFHQNAAGAVPSPFDCWLVLRGIRTLGIRMDRHAANAIAVAESLRAHPVVKRVF
                     YPGLPDHPGHDLARKQMGGFGGMVSFVVRGGLDAARKVASSTKVFILGESLGGVESLL
                     DHPASMTHASLSGSSLQVDGGLIRLSVGIEHVQDLVDDLRQALAKVR"
     gene            4335..6044
                     /locus_tag="AC159B_01210"
     CDS             4335..6044
                     /locus_tag="AC159B_01210"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - L-cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HIR3376405.1"
                     /translation="MPTIFIPAPMRRLMKGQGKVIVQGATVREVLTALERQYPGVRPE
                     VHEPGGEVRSFINIFVNGTEIRQLQGLDTPVQDADEVAIIPAMAGGAARRTKTRSHPG
                     RRRAVPAGRQEHIFDSILDSIGHTPLVRLHRVTHGIQADVVAKLEFLNPGGSVKDRIG
                     PKMIEAAEQTGRLRQGGTIVEATSGNTGVGIAITAAVRGYKTVFVMPDKMSDEKIRLL
                     RAFGARVVITPTAVPPSDPRSYYNVSRRIAEETPNSFYANQYANPANPEAHYATTGPE
                     IWAQTGGKIDILVVAMGTGGTISGAGRYLKERNPRLLVLGVDPIGSVFYEYFKTGRLP
                     EPHTYKVEGIGEDFLPETMDFSVVDEVIQITDRESFLMTRRLVREEGIFCGGSSGTAV
                     AGAIKYLRGRPDTAASLRVVVVFPDSGARYLSKIFSDDWMREHGFLDLDLGTAGELLR
                     LRPIDLVTAAAADAVSDVIAKMKQYDVSQLPVLDDGRLVGMISEVDLLNYLLEGSHRA
                     TDPIRSIIDPAPPVVSPDAPIDTLTDAFLTANAVVVVDHGAVVGIVTKIDVIDHLAKK
                     VSR"
     gene            6041..6877
                     /locus_tag="AC159B_01215"
     CDS             6041..6877
                     /locus_tag="AC159B_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376406.1"
                     /translation="MNQPSALRLATGQLLPDLRIAATEEVFVHEDHDPVRVDRLVASL
                     RADRVLRNQPIAATLSSGSYVVLDGANRAGALASIGIKAIPLQVVDYDDGRVELEVWH
                     HFVVDEIALFSKMSGAGLTLEAMSMREAQEALAGRALACYLVTKDNILGVRSGSPMGI
                     SLARVVAAYRGAARIYRVQGGAYAELVRNFGTDGTLIVFPSFTKTDIVAFARARAKLP
                     SGITRHVISGRALRLNLPLEALSKPDDLGEKNRWLSELIRQKLLDNRIRYYPEATFLF
                     DE"
     gene            6870..7817
                     /locus_tag="AC159B_01220"
     CDS             6870..7817
                     /locus_tag="AC159B_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HIR3376407.1"
                     /translation="MNKRAPRPRGPAGKPAIGQSILDRIGNTPLLRLERIMRDLPKRV
                     EVYAKAEWFNPGGSVKDRPVRRIIEDGEHDGRLAAGRVILDSSSGNAGIAYALIGAVK
                     GYRVRLVIPDNVSEERKRILQAFGAQVDYSDPLEGSDGAILAVRRLVEENPTAYFFGD
                     QYNNPSNPHAHYETTGPEVFAQTEGRITHFVAGLGTSGTLMGAGRALRMLSPEVQIVA
                     VEPDSGLHGIEGLKHMASAIVPGIYDPAAHHRKIQVRTEAAYGMAQRLAREEGLLVGQ
                     SSGAAMSGALEVARDLRDGVIVVVFPDGGDRYLSTALWR"
     gene            7889..8284
                     /locus_tag="AC159B_01225"
     CDS             7889..8284
                     /locus_tag="AC159B_01225"
                     /inference="COORDINATES: protein motif:HMM:NF025819.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008235 - metalloexopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HIR3376408.1"
                     /translation="MLELTQDQAAALVAHAREAFPNECCGLLAGRQGRVNRIFRGTNV
                     DRSPYTYLMDPKEQLVAFKDMEAAGLDLLAIYHSHTHTAAYPSKTDVAKAYYPDAIYV
                     IISLKDQQPSIKGYRIMDGRIADEKVVVR"
     gene            8281..8709
                     /locus_tag="AC159B_01230"
     CDS             8281..8709
                     /locus_tag="AC159B_01230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR3376409.1"
                     /translation="MKVSTRAEYGLRALIDLANHYGEGPIQTHDIARRQGLPEPYLNQ
                     LMTTLRRAGLVNSKRGPTGGHTLARAPADISLRNAFDVLEGSTAPWWCVETEDPNCQY
                     APGCGLRPVWQAVNSAVEGVLNSMTIADISPGVRQLAGKA"
     gene            8770..9438
                     /locus_tag="AC159B_01235"
     CDS             8770..9438
                     /locus_tag="AC159B_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3376410.1"
                     /translation="MGVVALAEQVPIPASADRQQFEQMVEEHLSGLFGAALRLTRNRT
                     NAEDLLQETFLRAWRSFHTFRPGTNARAWLYRILMNAYIDNYRKATREPEVVDQDDVD
                     EFYLYSKVQESDEYRRAGNPEEAFLQTLMDADVKAALESLPEAFRAVVMLADIDGFSY
                     KEIAEILSIPIGTVMSRLHRGRKQLQVALWEYARRARYVNGTRPQPPEPRSKGRPSAG
                     EERR"
     gene            9435..9665
                     /gene="rsrA"
                     /locus_tag="AC159B_01240"
     CDS             9435..9665
                     /gene="rsrA"
                     /locus_tag="AC159B_01240"
                     /inference="COORDINATES: protein motif:HMM:TIGR03988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol system anti-sigma-R factor"
                     /protein_id="HIR3376411.1"
                     /translation="MRELDCEQVLEMVWGYLDGEIDEEQYLGIHTHIEKCGECGPRYE
                     FQRKLMALVERKCKEGPVPADLKQRLFKLLKD"
     gene            complement(9666..10307)
                     /locus_tag="AC159B_01245"
     CDS             complement(9666..10307)
                     /locus_tag="AC159B_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3376412.1"
                     /translation="MIRVLLVDDHRLVAESIARLLEEYSDLQVVGQAGTGPEAISLAR
                     HLNPTVILMDIGLPGMDGVDATWTLKRQFPAIPVLMLTMFDHEAYAIESLRAGASGYL
                     VKTAGPDELVQAIRAVCAGKSVLPPQIASAALQRLATRGRLHARDPFALTQREIQILQ
                     LMVSGLNALEISRRLVLSPHTVRNHLKSVYKKLGVHDRTEAAIHAYRQGIAKA"
     gene            10460..10801
                     /locus_tag="AC159B_01250"
     CDS             10460..10801
                     /locus_tag="AC159B_01250"
                     /inference="COORDINATES: protein motif:HMM:NF016296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIR3376413.1"
                     /translation="MSQSSLDRLPVRVNGEIKLLPLVDVFYCYANGNRTVVVTARGEL
                     SSHGGLRRLAERLEPWGFFRCHRAYVVNLGHIRSVIPWTRNAYSLLLEGNREIPLSKH
                     RVTALRRVLDW"
BASE COUNT         2047 a         3331 c         3391 g         2080 t
ORIGIN      
        1 aggccggtcg tgggtcgtag gtcgtgggtc gtggaacctg caacctacca ccaccaccta
       61 cgacctacca cctacgaccg acgttgaggg acagcgttcg gcaggaggct gtccgccgaa
      121 caagcgaacg ggacgggaaa ttgtccccgg gggattgtta ttgactgagg tcaagaattc
      181 cgatagcctg agcggggatc aaatttctac cgggaaaggt gcatggcggg ctcatttctg
      241 atcacactcc gtgagggcct ggaggccgcg ctaatcgtcg gcattatcct tgcctatctc
      301 gcaaagaccg gcaacagggg caaatccgga gtcgtctgga tgggggtcgg cctgtccgtg
      361 ctcatgagcg tgctggtcgg cgccgccatc ttcttgacgg ccggcggcct ggcgggtcgg
      421 tctgaagcac ggttcgaggg cgtcgcgatg ttcactgcag caggcgtctt gacatatatg
      481 atcttctgga tgcgaaagca ggccatcaat atccgcgcgc acctccagcg gcagctcagc
      541 acggcattag aaagcgggtc gctcactgcg ctgggcctgc tcgctttcgt cgccgtcggt
      601 cgagaaggca ttgagaccgc gctcttcatg tttgccgcca cgaaggccgc gacaccggct
      661 gccgccacgc tgggtggtct cctggggttg gtcgctgctg tcgcgctcgg ttacgtcctg
      721 taccggggaa cgtatcgtct gaaccttcgc gcgttcttca acgtgaccag cgttctgctg
      781 ctgttgtttg ctgccgggct gctcgcgcac ggcattcacg agtttcatga ggcagccgtc
      841 atcccgcccg tggttgacga gctgtggaac acgaaccata ttctcgacga gcaatcgacc
      901 gtcggaagtt tcgccagggc gctcttcggg tataacggca acccctcgct cgtcgaagcg
      961 ctggcctacc tgacatacct gttgttggtc ggctggatgt acttccgccc gcccacggcg
     1021 aaggaactca caccgggccg cgcaagcgcc tgactaccag tctattgggc ccacggcagg
     1081 gaactttgag agagctgctt cgagagtacg cttgatctcg gcaagcgcct cgggcgtctt
     1141 gccttccgcg cggagcacga gagccggctg ggtgttgctg gcgcggatca agccccaccc
     1201 atccgggaag agcacgcgcg cgccatcgat atcgatgacc tcgtaggttg cgcggaactc
     1261 gtcggtgagg cgccggacga cctcgaactt cccttcatcc gggcaatcga cccgcacctc
     1321 cggcgtgacc gggtaccggg gcacatccgc gagcagcgcg gaaagtggac gaggtgagtt
     1381 tgacagtata cgcagcagcc tcgccgcagc atagaccgcg tcgtcgtagc cgtagtactc
     1441 gtcggcgaag aacatgtgtc cggacatttc gcccgtaaac acggcgccga tctccttcat
     1501 cttggccttg atcagggagt gaccggtccg gtaaaacatg ggtttcccgc cgaggcgcgc
     1561 gatctcgtcg tagagaacct gggagcactt gacctcgaca atgcccggcg tgcccgggta
     1621 cttgggaaga atctcgcgcc agaacacgac catgagcatg tcgccccaca gaatcgtgcc
     1681 ctgatcgtcc accacaccga tccggtcgcc gtctccatcg aatccgaccc cgacgtccgc
     1741 cttctcgcgg cgcacggtgg cgatcaagtc gacgaggttg tcggggtcga cagggtcggg
     1801 atggtggttc ggaaacttgg gatcgggatc acagtataga cgaatgacct cgacgccgag
     1861 atcttccagc gcctgcggcg cgatgaggct ggccgtgccg ttgccgcagt ccaccacggc
     1921 acgcaaccgt cgactcccca gtcgaatctt ttccttgagc atggtccggt acgcctggag
     1981 cacatcgtgc ttttcaacgc gcccgcggcc cgagacaaac tctcctgagc cggcgatgcg
     2041 gcgaatctcc tggatctcct cgccatagat cgtgccgaag ccgtgggcta atttgaaccc
     2101 gttgaactcg ggtggattgt ggctcgcggt aatcatgagg ccgccgtcga tgccgaggtg
     2161 cacgcgggcg aaatagaaca ccggtgtgat gacggtcccg atatcgacga tgtcgcagcc
     2221 gctgtcgatc aaggcggcta ccgcagcgtc gcgaatttcg ggcgagcttt cccggagatc
     2281 ccggcccacg acgaccttgc gctcgccgcg ctcactgagg tatgaggcgt acgcgcgcgc
     2341 gatcgcccct gcggtgtcga ccgtgatctc gcggcccacc acgccgcgga tatcgtactc
     2401 gcggaaaatt gtcggcgtaa tctgaggacg catgggacgc ttccatatta tagactcggt
     2461 agactagaat tgacctgaca tgcggccggg ctggctcctc aacgtgacag atcgtgtgac
     2521 accgtacgcg cacgcctggg atctgcaaaa gcagttagtg gaagccaggc agcacggccg
     2581 actcggcgac ggcctcgtct tggtgcaaca tgagccggtc ttcacgctgg gccggtcctc
     2641 tcgctccgag cacctcccat ttccccggcg ggctcttgcc caactcggct tcggcgtctt
     2701 cgagatcgag cgtggcggga gcgtgacgta ccacggcccg ggacaactgg tcggatatcc
     2761 cattcttgat ttgcgtagtt atggcgatga tgtcgtacag tttgtgcgct cgctggaaga
     2821 gagcgtcctc ggtaccctcg ccgatttcga cattgaggct cagcggatac gcgggttccc
     2881 cggtgtgtgg gcggggaatg agaagattgc ggcggtcggc gtcgccgtga agcgcaaggt
     2941 caccatgcat gggtttgcac tgaatgtcga ccccgacctc tcgcactttg cctacatcaa
     3001 tccgtgcggg ctcaacaagc ccgtgacctc ggtggcgaag atcctgggcc gcccggtttc
     3061 tctcgccgag gtcggactgt catacgcgcg ccgtttcagt gaggtgtacg gactgacaat
     3121 gagttcgatg ctgcccgagg cgctgctgtc tgccgcatcc gaatcgcccg ttccggtctg
     3181 agccttccat gacccggcgc cgcttcgaga cacgcgccat tcacgacggg caggatccag
     3241 acccgacgac agggtcggtt atccccccga tttaccagac ctccacgttt cagcagtcgg
     3301 cgcccgccga gcataaggga tatgagtacg cgcgcaccgg aaacccaacc cggacggcgc
     3361 tggaagcctg cctcgcgtcg ctcgaagatg gtcagttcgg ccttgccttt gcgtccggga
     3421 tggcggcgat tactacgacg gcgtatcttc ttagtcccgg tgatcatgcc gttctcccgg
     3481 atgacgtcta cggaggaacc tatcgtcttt ttgtgcgcgt gctggagcgc tacggcatcg
     3541 cctcgtcgtt tgtcgacatg accgatctca ctcgggtcga ggccgccctg acacctaaga
     3601 cacggatggt cctcaccgag acgccgacca acccctacct caaaattctt gacatcgccg
     3661 cgatcagtga actcgctcac gcccgcgacg ccctcgtcgt ggtcgacaac acgtttgcgt
     3721 ccccgtactt ccagcggccg ctgtcgctgg gcgccgatct cgtggtgcac tccaccacaa
     3781 agtatctggg cgggcacagt gacgtgatcg gcggcgctgt tgttgtgaac agcaacgaca
     3841 tctaccagac gctcaagttc catcagaatg ccgctggggc agtgccgagt ccgttcgact
     3901 gctggctggt cttgcgaggg atccgaacgc ttggaatccg aatggaccgg cacgccgcaa
     3961 atgcgatcgc ggtcgcagaa tccctccggg cccaccctgt tgtgaaacgg gtgttctatc
     4021 cgggcctgcc tgaccatccg ggccacgatc tcgcgagaaa gcagatgggc ggattcggtg
     4081 gaatggtgtc gttcgtcgtg cggggaggtc tcgatgccgc aaggaaagtc gcctcgtcga
     4141 ccaaggtctt catccttgga gagtctctcg ggggtgtgga atcgctccta gatcaccccg
     4201 ccagcatgac ccacgcgagc ctgagcgggt cttccctgca ggtggacggt ggcctcatca
     4261 ggctctccgt cggcatagag cacgttcagg acctcgttga cgacctccgg caggcccttg
     4321 cgaaggtgag atgaatgccc accattttta ttccggcgcc gatgcgccgt ctcatgaagg
     4381 ggcagggaaa ggtgatcgtt caaggcgcga cggtgcggga ggtgctgacc gctctggagc
     4441 ggcaatatcc gggggtccga cccgaggtgc acgagccggg cggcgaggtc cgcagcttca
     4501 tcaacatctt cgtcaacggc accgagatcc gccaactcca gggactggac acgccggtcc
     4561 aggatgcgga tgaagtggcg atcattcccg cgatggcggg aggtgcggcc cgccggacca
     4621 agacgcgatc gcatccgggt cgccgccggg ccgtcccagc gggtcggcag gagcatatct
     4681 ttgattccat cctggattcg atcggtcata cgccgcttgt ccgcctgcat cgtgtgacgc
     4741 atggcatcca ggccgacgtg gtcgcgaagc tggagtttct caaccccggg ggctcggtca
     4801 aagatcggat cggtcccaag atgatcgaag cggccgagca gaccggccgg ctgcgccagg
     4861 gcgggacaat cgtcgaggcc acgtcaggga acaccggcgt ggggatcgcc atcacggcgg
     4921 ccgtccgcgg atacaaaacc gtgtttgtga tgccggacaa gatgtcggac gaaaagatcc
     4981 ggctgctgcg cgcgttcgga gcgcgggtcg tgatcacgcc aacggcggtc ccacccagtg
     5041 atccgcgtag ttactacaac gtcagccggc gcatcgcaga agaaacgccc aatagtttct
     5101 acgcaaatca gtacgcgaac ccagcgaacc ccgaagccca ctacgccacg actggaccag
     5161 agatttgggc tcagaccggc gggaagatcg acatcttggt cgtggcgatg ggtactggcg
     5221 gcaccatcag cggtgcaggc cggtacttga aagagcgcaa cccacgactg ctggtcctgg
     5281 gcgtcgatcc gatcggatcg gtgttctatg aatacttcaa gaccggccgg ttgcccgagc
     5341 cgcacacgta caaagtcgag gggattggtg aagacttcct ccccgagacg atggacttct
     5401 cggtggtcga tgaggtcatc cagattaccg accgtgagtc gttcttgatg acccggcgcc
     5461 ttgtccgcga agagggcatt ttctgcgggg gcagcagcgg aaccgcggtc gccggcgcga
     5521 tcaagtatct gcgcggccga cctgacaccg ctgcctctct tcgtgtcgtc gttgtattcc
     5581 ccgactcagg cgcgcggtat ctctcgaaga tcttctcgga tgactggatg cgcgagcacg
     5641 gctttctcga tctcgatctg ggcacggcgg gtgaactgct ccggctgcgc ccgattgatc
     5701 ttgtgacggc cgccgccgcc gatgcggtca gcgatgtcat cgcgaagatg aaacaatacg
     5761 atgtctcgca gttgcctgtt ctcgatgatg ggcgtctggt cgggatgatc agcgaagtgg
     5821 acctcttgaa ctaccttctt gagggttcgc accgggccac cgatccgatt cgctcgatca
     5881 tcgacccagc gccgccggtc gtctcgcccg atgcccccat cgatacactc accgacgctt
     5941 tcctcacagc caacgccgtc gttgtcgtgg atcatggcgc cgttgtcggg atcgtcacca
     6001 agatcgacgt tatcgaccac ctggcgaaga aggtcagtcg atgaatcagc catcggccct
     6061 gcggctggcg acgggccagc tcctgcccga cttgcgcatc gccgcgaccg aagaggtctt
     6121 tgtccatgaa gaccatgatc cggtacgggt ggaccggttg gtcgcatctc tccgcgccga
     6181 tagggtgctg cgcaatcagc caatcgccgc gacgctttca agcgggagct acgtcgttct
     6241 cgacggcgcc aaccgcgcgg gcgctctagc gtccatcggc atcaaggcga tcccgttgca
     6301 agttgtggac tatgacgatg ggagggtgga gctcgaggtc tggcatcact tcgtggtcga
     6361 cgagatcgct ctcttttcca aaatgtcagg agccggattg acgctcgagg ccatgagcat
     6421 gcgagaagct caagaggccc tggccgggcg cgccctcgcc tgctatctcg tgacgaaaga
     6481 taacattctc ggcgtccggt cagggagccc catgggaatc tcgctcgcgc gcgtggttgc
     6541 cgcctaccgg ggtgcggcac gcatctaccg tgtgcagggg ggcgcctatg ctgaattagt
     6601 ccggaacttc ggaacggacg gcacgctgat tgtctttccg tcattcacga agacggacat
     6661 cgtggcgttc gcgcgagcac gagcgaaatt gccttcgggc attacacgcc acgtcatctc
     6721 cggacgcgcc ctgcgcctca accttcccct ggaggccctc tcgaaaccgg atgatctcgg
     6781 ggagaaaaat cgctggctct ctgaactcat ccggcaaaag cttctggata acaggatccg
     6841 ttactacccc gaggcgacct ttctctttga tgaataagcg ggccccgagg cctcggggcc
     6901 cggctggaaa gccagccatc ggtcaatcca tcctcgaccg catcggcaac accccgttgc
     6961 tccggttgga acggatcatg cgcgatctcc ccaagcgcgt cgaggtctat gcgaaggccg
     7021 agtggttcaa ccccggcggg tcggtcaagg accgcccggt gcgccgcatc atcgaggacg
     7081 gcgagcacga cggccgtctc gcagccggtc gagtcatcct tgactcgtca tctggaaatg
     7141 ccggcatcgc ttacgccctg atcggggcgg taaaggggta tcgcgtccgg ctggtaatcc
     7201 ccgacaacgt gagcgaggaa cgcaagcgga tcctccaggc gtttggagcg caggtggact
     7261 attcggatcc actggaagga tctgacggag cgattctggc cgtccgccgt ttggttgagg
     7321 agaatccgac agcatatttc tttggagacc aatataacaa cccaagcaat ccccatgcgc
     7381 actacgagac gaccggtccc gaggtgttcg cgcagacgga aggtcggatc actcactttg
     7441 tcgctggcct gggcacaagc ggcacgctca tgggggcggg acgcgctttg cgcatgctga
     7501 gtcccgaggt tcaaattgtc gcggtcgaac cggacagcgg gctgcatggg atcgaagggc
     7561 tcaagcacat ggcctcagcc atcgtgccag gcatctatga tccggcggcg catcaccgca
     7621 agattcaggt acgcaccgag gcggcgtatg ggatggcgca gcgactcgcg cgggaggagg
     7681 ggctgctcgt cgggcaatcc tcgggcgcgg ccatgtcagg cgccctggag gtggcccgtg
     7741 acctgcgcga cggcgttatc gtcgtcgtgt tccctgacgg cggagaccga tacctttcga
     7801 ccgctctctg gcgctaaccc gggtcccttt gtcctgtgcc ccgatccccg ctgtccaaat
     7861 gcatcttgtg ctataaaggg ggctaagagt gttggagctc acccaggatc aggctgccgc
     7921 gcttgtggcc catgcgaggg aggcatttcc caatgaatgc tgcgggttgc tggcgggacg
     7981 tcagggtcgc gtcaaccgga tctttcgggg aacgaacgtc gatcggagcc cttatactta
     8041 tctcatggat ccaaaagagc aactggtggc gttcaaggac atggaagcgg cggggttgga
     8101 ccttctggcc atctaccatt cacacaccca tactgccgcc tatccgtcca aaacggacgt
     8161 ggcgaaggca tactatcctg acgcaatcta cgtgatcatc tcgttgaaag atcaacagcc
     8221 gtctattaag gggtatcgca tcatggatgg gcggattgcg gacgagaagg tggtcgttcg
     8281 atgaaggtgt cgacgcgcgc cgagtatggt cttcgcgcac tcatcgacct cgccaaccat
     8341 tacggcgagg ggccgatcca aacccacgac atcgcacgcc gccaggggct gccggaaccc
     8401 tatctcaatc agttgatgac aacccttcgc cgggcgggac tcgtgaacag caagcgaggc
     8461 cccacaggtg ggcatacgct cgcgcgcgcg cccgcggaca tttctttgcg gaacgccttt
     8521 gacgtcctcg aagggtcgac cgcgccctgg tggtgcgtgg aaaccgagga tcccaactgt
     8581 cagtatgccc ccggctgtgg cctgcgtccc gtctggcagg ccgtcaactc cgccgtcgaa
     8641 ggggtcttga actcgatgac catagcagat atcagccctg gagttcgtca gctagcgggc
     8701 aaagcgtagc ctccacgatc tctcctcggg aattcagcgg acaccgggag cgttctaccg
     8761 gtggtcgcca tgggagtcgt tgcgttggct gaacaggtcc caatcccggc aagcgccgat
     8821 cgccagcagt tcgagcaaat ggttgaggag cacctcagcg ggctgtttgg ggcggcgctg
     8881 cggttaacgc gcaaccgcac gaacgccgag gatctcctgc aagagacctt cctccgcgcc
     8941 tggcgctcgt tccacacgtt ccggccgggc acgaatgccc gcgcgtggct ctaccgcatc
     9001 ctcatgaatg cgtacatcga caactaccgg aaggcgaccc gggagccgga agtggtcgat
     9061 caggatgacg tcgacgagtt ctatctgtac tcgaaagtgc aggagagcga cgaataccgc
     9121 cgcgccggaa accccgaaga agcgttcctg cagacactta tggacgcgga tgtcaaagcg
     9181 gcgctggaaa gtctccccga agcctttcgc gccgtggtca tgctcgccga tatcgacggg
     9241 ttctcgtaca aagagatcgc tgagattttg agcatcccca tcgggaccgt gatgtcgcgc
     9301 ctgcaccggg gccggaagca attgcaggtg gcgctctggg aatacgcgcg ccgcgctcgc
     9361 tacgtcaatg ggacgcgtcc gcagcccccg gaaccccgga gtaagggtcg cccgtccgcg
     9421 ggtgaagagc gccgatgagg gaactggact gcgagcaggt cctggagatg gtctgggggt
     9481 acctcgacgg tgagatcgac gaggaacaat accttgggat ccacacgcac atcgagaaat
     9541 gcggagaatg cggtccccgc tatgagtttc agcggaaact catggccctt gttgaacgaa
     9601 agtgcaaaga aggccctgtt cctgctgacc tcaaacagag actgttcaaa ctcctgaaag
     9661 actagtcacg ctttcgcgat tccctggcga taggcgtgga tcgccgcttc agtccggtcg
     9721 tgcactccca gcttcttgta gacagatttc agatggttgc gtacggtgtg cggactcagc
     9781 acgagccgcc ggctgatttc cagcgcattg agcccgctga ccatgagctg taggatctga
     9841 atctcccgct gggtcagtgc gaacggatcg cgcgcatgca gccgcccacg ggttgccaga
     9901 cgctggagcg ccgcggacgc gatctgtggg ggcagcacgg acttgcccgc gcagacggcg
     9961 cggatcgcct ggaccagctc atccgggccg gctgtcttca cgaggtaccc ggacgctccc
    10021 gcccgcagcg attcgatcgc atacgcctca tggtcgaaca tcgtcagcat gagcacggga
    10081 attgccggaa actgccgctt gagcgtccag gtggcgtcca ccccgtccat gccggggagc
    10141 cctatgtcca tcaggatcac agttggattg agatgacggg caagagagat ggcttcgggg
    10201 ccggtgccgg cctgacccac gacctgcaag tcggagtact cctcgaggag cctggcgatc
    10261 gactccgcaa cgaggcggtg atcgtcaacg aggaggacgc ggatcatagt caaaggaacc
    10321 ccacgccctc cgcccatcta tattaagcga gatcaatctt atgtaaagaa cacgatcttc
    10381 aattgactca cgaaggctag ttcgtgacgg ccgaacggag ccggggcaca aagcgctctt
    10441 cctgaagtat gctgagcgcg tgtcacagtc gtctcttgat cggctgcctg tgcgcgtcaa
    10501 cggggagatc aagctgcttc ccctggtgga cgtcttctac tgctatgcga acggcaaccg
    10561 gacggtcgtt gtcaccgcgc gtggagaact gtccagccac ggaggacttc gccgcctggc
    10621 ggaacggctg gagccgtggg gattctttcg ttgccaccgc gcctacgttg tgaacctggg
    10681 ccatatccgc agcgtcatcc cgtggactcg aaacgcatac agcttgctgc tggagggcaa
    10741 tcgagagatc cccttgagca agcaccgcgt gaccgcgctg cgccgggtgc tggactggta
    10801 gtagtttcag gtggcgaccc accaggatag cctgcgtagc aggccggcg
//
LOCUS       DBNGSB010000043         4122 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_335407, whole genome shotgun sequence.
ACCESSION   DBNGSB010000043 DBNGSB010000000
VERSION     DBNGSB010000043.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_335407"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="AC159B_01255"
     CDS             <1..449
                     /locus_tag="AC159B_01255"
                     /inference="COORDINATES: protein motif:HMM:NF019522.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HIR3376414.1"
                     /translation="FGAGTEEAFYLQLGPLEQLNLRGLLLGGIILGALGVLDDITTAQ
                     AAAVDEIHRANPALSARELYQRGVSVGTEHITSLVNTLFLAYAGVSLPLFLLFTLSED
                     RPLWVLVNSEFIAEEVVRTMAGSIALVLAVPITTVLAAKALGRASS"
     gene            complement(439..1371)
                     /locus_tag="AC159B_01260"
     CDS             complement(439..1371)
                     /locus_tag="AC159B_01260"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR3376415.1"
                     /translation="MSDLQRYIIEEWAEDYRGGRMSRREFLRRTALMAGGVTLAIPML
                     GSLGVSASADEVAQAASSGPSTVAQASGVTVPENDPAIAAGMVSYASGGTAVVAYQAR
                     PRTGGPAPGVLVIHENRGLLEHFKDVARRLAKVGYVAIAPDLASHQGGTARYSDPAQV
                     TAILGQTSAELMVELLNSGVLYLQNQSTVRRDRIGVMGYCFGGGIVWRLATRNADLKA
                     AAPYYGANPPLEDVPKIKAAVLAIYGEQDTRINAGIPAIRDALQKASVPHEIVVYPNA
                     DHAFFNDTGTRYQAQAAQAAWQRTLAWYERYLKS"
     gene            complement(1470..2480)
                     /locus_tag="AC159B_01265"
     CDS             complement(1470..2480)
                     /locus_tag="AC159B_01265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR3376416.1"
                     /translation="MKVDARITYATLREVPTLAGAAEEIGVDGIWFTETSHDPFLPAV
                     LAAEHTARVSIGTAVAIAFTRSPTLLAYLAWDLAAQSGGRFVLGLGTQVKAHIIRRFG
                     MTWDPPAPKLRESVQAIHAVWDAWRTRDPLRFRGRFYNLSLMTPFFTPAPLDAKIPIF
                     TAGVNPPLCRIAGQVADGFVVHPFHTRAYLRDVVLPAVRTGRTKTGRDTVPFTIAATV
                     FTATGETAGDVARARDEVRRMVAFYASTPSYRRVLAHHGWQEVGERLSGRAARGEWTA
                     MAAEVSDEMLEAVAVIGRAEEIGPEIMDRYRGLVDRIGFYEPFVPADLKKWRSLLDAA
                     RG"
     gene            2653..>4122
                     /locus_tag="AC159B_01270"
     CDS             2653..>4122
                     /locus_tag="AC159B_01270"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF019450.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIR3376417.1"
                     /translation="MRLGSAALLAVLIAWTLPAGVALGQAAGVKVIVNGVEISLPAPA
                     VLSGGQVMAPISGLFEPMGAVAAFYETDRTIVVSNRVRTTVRMRLGDPTLYVNAAARA
                     VPVAPTQRGPHVFVPAQAVFAALGAWTKYEEAERTLYVSSQIKSIVTQASRDVLQIRI
                     DATGPVRVETNVLSNPDRLVVDLLHAALRTYERESPINSAGVRRIRTAQFQVKPYISR
                     MVFDLAQPAEIRVTTSPTNYVVTLEVRPKDAGIIGRPRPPAPTLGERDSNHGAKIAGV
                     TFQESGQAGRLFIEGNGTMEYRVREFVYPDRLVIDVQDAVFIPIKQDISVDGASIVSV
                     RAAQFTAEPPVTRIVVTLKRKMNYVVSQSDGRLAIEIKAFTARGHLVALDAGHGGRDP
                     GAIGPSGLRESDAVLDIAMRARELLQRDGIRVWMVRETDANVELADRPRLTRDAGATI
                     LVSIHANAHTRTAVNGSETYYLTPQSLALAQMIQDELGVV"
BASE COUNT          761 a         1290 c         1326 g          745 t
ORIGIN      
        1 tctttggggc ggggaccgag gaagcgttct acttacagct aggtccgctg gaacagctca
       61 accttcgcgg gctgctgctg ggcgggatca tcctgggggc gctgggcgtt ctggacgaca
      121 tcacaaccgc tcaggcggcg gccgtcgacg aaatccaccg cgccaacccg gcgctctcag
      181 cgcgggaact ctatcagcgc ggagtctcgg tcgggaccga gcacattacc tcgctggtca
      241 acacgttgtt cctcgcctac gcaggcgtat cgctgccgtt atttctgctc ttcacgctca
      301 gcgaggaccg gccactctgg gtcctcgtca atagcgagtt catcgccgag gaagtggtac
      361 gcacgatggc cggcagcatc gctctggtgc tggcggtgcc gatcaccacc gtactcgcag
      421 cgaaagcgct ggggcgcgct agctcttgag gtagcgttcg taccaggcga gcgtgcgctg
      481 ccacgccgcc tgcgcggcct gcgcctgata ccgggtcccc gtgtcattga agaatgcatg
      541 gtcggcgtta ggatagacca cgatctcgtg cgggacgctg gccttctgga gcgcgtcgcg
      601 gatcgcggga atcccggcat tgatgcgagt atcctgctcg ccgtagatgg cgagcacggc
      661 cgctttgatc ttgggcacgt cttcgagcgg cgggttggcg ccataatagg gagccgctgc
      721 cttcaggtcg gcattgcgag tggccaagcg ccagacaatt ccgccgccga agcagtaccc
      781 catcacgccg atgcggtccc gacggacggt ggattggttc tggagataga gcacaccgga
      841 gttgagcaac tcgaccatca gttccgccga ggtctgtccg aggatcgccg tgacctgcgc
      901 aggatcgctg tagcgcgcgg taccgccttg atgcgacgcc agatcaggtg cgatggcgac
      961 ataaccaacc ttggcgagcc gacgggcgac gtctttgaaa tgctcgagca gcccccggtt
     1021 ctcgtggata accaacacgc ccggcgcagg cccaccagtt ctcggcctgg cctgatatgc
     1081 aaccaccgcg gtgccaccgg atgcgtacga caccatcccg gctgcgatgg cgggatcatt
     1141 ctctggcacc gtcacgcccg acgcctgtgc aaccgtcgac ggcccagatg aggcagcctg
     1201 agcgacttca tcggccgacg cagagacgcc gagactgccg agcattggaa tcgcgagcgt
     1261 gacaccgccc gccattaaag ccgtgcggcg caggaactcg cgccggctca tccggccgcc
     1321 gcgataatcc tctgcccatt cctcaatgat gtaacgctgg agatcactca ttgaagcctc
     1381 ccttaagaga tgtgtggatc aatggtctga gggatctgta acgattccat cactccctct
     1441 tctatgaccg tgaggactgc cagatgcgtt tagccgcggg ccgcgtccag gagagagcgc
     1501 cactttttca agtcggccgg cacaaagggc tcgtagaatc cgatgcggtc gacaagtccg
     1561 cgataccggt ccatgatctc aggcccgatc tcctcggccc ggccgatgac ggcgacggcc
     1621 tcgagcattt catcggacac ttccgctgcc atcgcggtcc attcgcctcg tgcggctcgt
     1681 ccactcaagc gctcgccgac ctcctgccat ccgtgatgcg caagcacgcg tcgataggat
     1741 ggggtgctgg cgtaaaacgc aaccattcgc cggacctcgt cacgcgcacg ggcgacgtcc
     1801 cccgctgttt cgccggtcgc cgtgaagacc gtcgcagcga tggtgaacgg taccgtgtcg
     1861 cgtccggttt tcgtccgccc ggtgcggacg gccgggagga ccacgtcacg caggtaggcg
     1921 cgcgtgtgaa atggatgcac gacgaatccg tcggcgacct ggccggcaat ccggcacagc
     1981 ggtgggttga cgccggcggt gaaaatcggg atcttcgcgt cgaggggcgc gggggtgaag
     2041 aatggcgtca tgagcgatag attgtagaat cggcctcgga accgcaacgg atctcttgtc
     2101 cgccacgcgt cccataccgc gtggattgcc tgaacagatt ctcggagttt tggtgccggc
     2161 ggatcccacg tcatgccgaa acggcggatg atgtgcgcct tcacctgtgt tcccaggcca
     2221 aggacgaacc gacctccaga ctgggcggcc agatcccagg ccagataggc gaggagggta
     2281 gggctgcgtg taaaggcaat agcgacggcc gtcccgatcg atacgcgcgc ggtgtgctcg
     2341 gcggcaagca cggccggaag aaatggatcg tgcgaggtct ccgtaaacca gattccgtcg
     2401 acgccgatct cctcggccgc accggccaga gttggaacct ctcgaagggt cgcgtacgtg
     2461 atacgggcat ccactttcat gagggtcaaa ggtcactgtt ctcatgtctg acgcgtgtcc
     2521 ctgtgcgaag gaattccgga tgccgggcgg aacgatgttc ggttgggaga ggacagggca
     2581 ggatggctcc agtgattccc gcgccagcgg gagtctcgat tggaaggtgg taggcgcacg
     2641 cagaggaaga gcatgaggct cggatctgcc gcgctcctgg cggtactgat tgcctggacg
     2701 ctgcccgcag gggtggccct cgggcaagcc gccggagtga aagttattgt gaacggcgtc
     2761 gagatctcac tgcccgcgcc tgccgtcctc tccggcggtc aggtgatggc tcccatttcg
     2821 gggctgtttg agcccatggg ggccgtggcg gcattttacg agaccgaccg cacgatcgtc
     2881 gtcagcaacc gtgtgcgcac aacggtgcga atgcggttgg gtgatcctac cttatatgtg
     2941 aacgccgcgg cgcgggcggt gccggtcgcc ccgacgcagc gaggtcctca cgtgttcgtc
     3001 cccgctcagg ccgttttcgc cgcgctcggc gcctggacaa agtacgaaga agcggagcgg
     3061 actctctacg tcagctctca gatcaagagt atcgtcaccc aggcatcgcg agacgtacta
     3121 cagatccgta tcgacgccac gggtccggtg cgggtagaga cgaatgtgct cagcaaccct
     3181 gaccgcctgg tcgtagatct tctccacgcc gcgctccgca catacgaacg agagagtccg
     3241 attaacagcg ccggcgtgag gcggatccgg accgcccagt tccaggttaa gccctacatc
     3301 tcgagaatgg ttttcgatct tgcgcagccg gcggaaattc gcgtcaccac atcgcccacg
     3361 aattacgtcg ttaccctcga agtgcggcca aaggacgcgg ggatcatcgg gcgacccaga
     3421 ccgccggcgc cgacgctggg tgagcgagat tctaaccatg gcgcgaagat tgcgggcgtg
     3481 acgtttcaag agtccgggca ggcgggccgt ctgttcatcg aaggcaacgg aacgatggaa
     3541 taccgggtgc gcgagttcgt atatcccgac cgcctcgtca tcgacgttca ggatgccgtg
     3601 ttcattccca tcaaacaaga catatctgtc gacggcgcct cgatcgtctc cgtgcgcgcg
     3661 gcgcagttca ccgcggaacc tccggtgaca cgcatcgtgg tcacgctcaa gcgcaagatg
     3721 aactatgtcg tcagccagtc cgacggccgg ctggccatcg agatcaaggc gttcaccgcc
     3781 cgtggccacc tcgtcgcgct ggacgcggga cacggcggcc gcgatccggg tgcgatcggt
     3841 ccgtcggggc tgcgcgagtc cgatgccgta ctggacattg cgatgcgcgc gcgagagctg
     3901 ctgcagcgtg acggaatccg ggtctggatg gtccgggaga ccgacgcgaa cgtcgaactc
     3961 gccgatcgcc ccaggctgac ccgcgatgcc ggagccacaa tcctcgtcag cattcacgcg
     4021 aatgctcaca cgcgcacggc cgtcaacgga tcggagacat attacctgac cccccagagc
     4081 ctcgcgctgg cccaaatgat ccaggatgaa ttgggggtgg tc
//
LOCUS       DBNGSB010000044         5762 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3096639, whole genome shotgun sequence.
ACCESSION   DBNGSB010000044 DBNGSB010000000
VERSION     DBNGSB010000044.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 5762)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5762)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5762
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3096639"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="AC159B_01275"
     CDS             <1..624
                     /locus_tag="AC159B_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007748408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3376418.1"
                     /translation="DPRQAGRVFANRGVRLASFGYFGHMWELFAMWAWFVVFFSDELA
                     ARGRSVGSAAPYATFAVISIGALGSWLAGILAEQWGRTRTTALSLGISGTCCVLIGML
                     FGAPTWVVLLVSLVWGCAVVADSAQFSTMVTELADQAYVGTALTLQLAVGFALTVATI
                     WLIPFLENAFGWRWAFAFLAPGPILGVMAMLRLKSLPESTRIAGGLG"
     gene            complement(641..1219)
                     /locus_tag="AC159B_01280"
     CDS             complement(641..1219)
                     /locus_tag="AC159B_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HIR3376419.1"
                     /translation="MSKGRLEAFSDGVIAILITIMVLELKIPHGTGWEALRPVVSIFL
                     IYVLSFVFLGIYWNNHHHMLHAADRINGKVLWANLHLLFWLSLIPFVTGWMGENQFAQ
                     LPTAVYGGALLMAAIAYTILQAALVAAQGSNSRLAAAVGNDVKGRVSLLSYVAAIPLA
                     FVSQWIAIALYVFVALMWLIPDRRIESTVGRL"
     gene            1392..2408
                     /locus_tag="AC159B_01285"
     CDS             1392..2408
                     /locus_tag="AC159B_01285"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPL family radical SAM protein"
                     /protein_id="HIR3376420.1"
                     /translation="MAIGALALPETLSRPEETADTIASPRLFKATGARKTPQVEWVEI
                     HVKSVLNRVKGMPFEWSINPYRGCGHGCNFCYARVTHWYMDQDGVNDWSSRIFVKVNA
                     PEVLRSELARSDWTHEDVAVGTATDPYQPAEGAYRLTRRILEALRDFRTPISLITRSP
                     MVTRDIDLLQQMSRASGASVCFSIVTMDLELSQELEPHVAPPLKRLEALRTLAEAGVE
                     TSVNLAPVIPRITDNEQNLAEVIRAAKYYGASQLWTNALHLGEVVRDSFFKYLHEKHP
                     DLIPEYERMYPKRYAPKEYQDHVQRVVSALKSKIGFSGRGRRGDGTDDRPSREEPAQL
                     QLFG"
     gene            2619..3458
                     /locus_tag="AC159B_01290"
     CDS             2619..3458
                     /locus_tag="AC159B_01290"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3376421.1"
                     /translation="MNSRVESGYVPVTGARLYFEATGDGHPLVLIHAGIADCRMWDEQ
                     FTEFSKTYRVIRYDTRGFGKSKTADVEFSNRQDLFDLLAHLEIEKAHVLGASRAGGIA
                     LDFTLDHPGKVDALILVSSALGGLNHRTPDHEKRMFDEMNEAWDQKNFVRLAGLELKM
                     WVDGPGQPETRVASALRERVREMILGTYTSHTTEGKPQRLDPPAARRLAEVKAPTLII
                     FGDLDESGVLAAADELERGITAARKAVLAGTAHLPSMEKPHEFNRIVLDFLTDVSSEE
                     RSR"
     gene            3718..4434
                     /locus_tag="AC159B_01295"
     CDS             3718..4434
                     /locus_tag="AC159B_01295"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR3376422.1"
                     /translation="MSGEPQVKSELEPVRKTKVYEGVAGQIQRLIRDGRLNPGDKLPP
                     ERELAEAFAVSRTSVRDAIRVLEMQGMVEPRQGDGTVVRDLSPDSLVNPLTSLLARNG
                     RLLTELLDVRKMLEPPLAARAATHASEVDVNKLRAILDRQAEKVQRGELAIDEDTQFH
                     YMIATAAQNSVVMRLVDVLMDLLRESRERSLQVKGRLQKSLAGHRQIFAAIHSRDQAA
                     AEAAMRQHLQEIEDVIVGRS"
     gene            4437..5678
                     /locus_tag="AC159B_01300"
     CDS             4437..5678
                     /locus_tag="AC159B_01300"
                     /inference="COORDINATES: protein motif:HMM:NF026048.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster-binding protein"
                     /protein_id="HIR3376423.1"
                     /translation="MGRMFAVEIVYRGIFQKNLAKNISRGIALAAKHDGKTAISFGRY
                     GDSPERNGIPAKNFAIVATDGETLEEGMAKYEPKEVDISIAVDDTLCKGVESWAWSGL
                     QPINRLLKPGGTLIVTSMHEPEVLIDMAHRKETPYNLAIVKSIPSFAGLWVYKDDHTD
                     VRILGAIAKVLPDLFSIESVEKAILEEWHEPLKATSARKSYERITTRPVQPTEGSAEK
                     PYEFELPKWWEMREGLAIPSIHMGGEITDPTTQVSGGYRPDRNPTFKKYSTRTMRPVV
                     DFDTCVKCTLCWLQCPDSCFDVTPDGLYDANMQACCGCGVCEAVCPVDNCVTMVNESA
                     FPDNASQWEMYRQDKPGYKTWLQEKIQHKPDRSHGFNIRGQYERQIDTMIADGTIEGG
                     KEAITEKVNTDVGEASAGGLD"
BASE COUNT         1275 a         1622 c         1737 g         1128 t
ORIGIN      
        1 gatcccaggc aggccggccg agtcttcgcc aaccgaggtg tcaggctcgc ttcctttggg
       61 tacttcgggc acatgtggga actgtttgcg atgtgggcgt ggttcgtggt cttcttctcc
      121 gacgaactag ctgctcgagg gcgctccgtg ggatcggcgg ccccttacgc gacgtttgcc
      181 gtgatcagta ttggggcgct cggaagctgg cttgcgggca ttctcgccga acagtggggt
      241 aggaccagga cgaccgcatt gtcattggga atatcgggca catgctgtgt cctgattgga
      301 atgctattcg gcgcgccgac atgggtcgtt ctgctggtga gcctcgtatg gggttgcgcc
      361 gtcgtggccg actccgcgca gttctcaacg atggttaccg aattggcgga tcaggcctac
      421 gtggggacgg cgttgaccct gcaacttgcc gtcggcttcg cccttactgt ggccacgatc
      481 tggctgatcc cgttccttga gaacgccttt ggatggaggt gggcctttgc gtttctggcc
      541 cccggaccga tcctcggcgt gatggcaatg ttgcggctca aatcattgcc ggaatccact
      601 cgaatcgcgg gaggcctcgg ctaattggta ttgagccggc ttacaagcga cccacggtcg
      661 actcgatccg ccggtccgga atgagccaca tgagcgccac gaagacatag agcgcgatgg
      721 cgatccattg actgacaaac gccaaaggga ttgcggcaac gtacgaaagg agcgagaccc
      781 tgcccttcac atcgttgccc accgccgccg cgagcctcga gttcgagccc tgcgctgcaa
      841 cgagcgcggc ctgaagaatc gtgtacgcaa ttgcagccat gagcaatgca ccgccataga
      901 cggcagttgg cagttgcgca aactgatttt ctcccatcca gccggtgacg aatggtatca
      961 gcgacaacca gaacaagagg tgcaaattcg cccagagtac cttgccgttg attcgatcgg
     1021 ccgcatggag catgtggtga tggttgttcc agtagatgcc tagaaacaca aagctcagca
     1081 cgtagatcag aaatatggac actactggac gcagcgcctc ccagcctgtg ccgtgcggga
     1141 ttttcaattc cagcaccatg atggtgatca agatcgcgat gaccccatca ctaaacgctt
     1201 cgaggcgccc tttgctcatg ttttggtgat gcctattcta ttgcactttg caacttcatc
     1261 ttggaggatc tactcgaagc atgcggtgag ttgaggaaac atactcaggg cagactcgtg
     1321 agccctcaga ggagtacctc gactttcggc attgacacga actagtgttc ggtattaccc
     1381 tcggaaccag catggcaatt ggagcgctcg cgcttcccga aacactcagc cgaccagaag
     1441 agactgctga caccattgcg tcgccgcgct tgttcaaagc gacgggcgcg cggaagactc
     1501 cgcaagtcga gtgggtggaa attcatgtca agtccgttct caaccgcgtc aagggcatgc
     1561 cattcgagtg gtcgatcaat ccctatagag gatgcggtca tggctgcaat ttctgttacg
     1621 ctcgcgtaac acattggtac atggatcagg atggggtgaa cgattggtct tcacgaattt
     1681 tcgttaaggt gaatgcgccg gaggtgcttc gaagtgagct cgctcgctct gactggactc
     1741 acgaggatgt tgcagtgggc actgcgacgg atccctatca accggcagag ggtgcgtacc
     1801 ggttgacacg gaggattctg gaagccctgc gtgacttccg cactcccatc tccctaatca
     1861 cacgctcgcc gatggtcacc cgggacattg atctccttca gcaaatgtca cgagcttcgg
     1921 gggcttccgt ttgtttcagc atagtcacca tggatcttga actgtcgcag gagctcgagc
     1981 cgcacgtcgc gccaccactc aagcggctgg aggcgttgcg aacactcgca gaggcaggtg
     2041 tcgagacgtc agttaaccta gcaccggtca ttccccgcat cacggacaat gaacaaaacc
     2101 tagctgaagt gattagagcc gccaagtact atggggcttc ccagttgtgg acgaacgcac
     2161 tacatctggg cgaggtcgtg cgcgactcat tcttcaagta ccttcatgag aaacatccgg
     2221 atctaattcc tgagtacgag cgaatgtacc caaagcggta cgcgccgaag gagtatcagg
     2281 atcatgttca gcgcgtcgtt agcgcgttga aatccaagat cggattctca ggccgaggac
     2341 gtcggggcga cggcaccgac gacaggcctt ctcgggaaga gcccgcacaa ctgcaacttt
     2401 ttggctagct ggatgtttga acccggttag accgggcaaa aatactcggc cagcggaagc
     2461 gaatccaaga tggtggaagg tagctcttcg gagttgccca aaagctaact aggatagcgc
     2521 accgctggtt agtcttttcc tctgccggaa gtccccgctt gatggtttct tgatcaacac
     2581 tcaggggacc aaagctagga aaccttagga ggaacctgat gaactcacgt gtcgagagtg
     2641 ggtacgtgcc tgttactggg gcgcgattat acttcgaggc cacaggtgat ggccatcctc
     2701 tggttctaat tcacgccggc attgcggact gccggatgtg ggacgaacag ttcacagaat
     2761 tctcgaaaac ctatcgcgtc attcgttatg acactcgggg ctttggcaaa tcgaagaccg
     2821 cagacgtcga gttctcaaat cgccaggacc tcttcgatct ccttgcgcac cttgaaatag
     2881 aaaaagccca cgtccttggg gcatcacgag caggagggat cgccctcgac ttcaccctcg
     2941 accatcccgg gaaagtggac gctctgatcc tggtgagttc tgctctcggt ggcctcaatc
     3001 atcgaacccc cgaccatgag aagcggatgt tcgacgaaat gaatgaagcg tgggatcaaa
     3061 agaacttcgt ccggctcgcc ggcctggaac tgaaaatgtg ggtggacggc ccgggccagc
     3121 ccgaaacccg cgttgcttcc gcattgcgcg aacgtgtacg cgagatgatc ctggggacct
     3181 acacctccca cacgacagaa gggaagccgc agcgccttga ccctccggca gccagaagac
     3241 tcgccgaagt caaagcgccc accttgatca tctttggaga cctcgatgaa tctggcgtcc
     3301 tggcagccgc tgatgagctg gagcgcggca tcactgcggc gaggaaggcc gtcttagccg
     3361 ggactgcaca cttgcccagc atggaaaagc cccacgagtt caaccggatc gtcctcgact
     3421 tcctgacgga tgtctcgtca gaggagcgca gccggtagtg ggtcagtttg aaagcaagcg
     3481 cgagtggcgc gctgcttctg agactgtcat actcgtgaca tggcgaagcg gaagcgcaag
     3541 aggcatctcc gaaagcactg gcagggaaag cggaagagtg aaggtgaaag gcgcaggacg
     3601 gagattcgga agaagaaccg cgcagccgga ggtcttgacc gccagggaaa tggcatgaga
     3661 tagtggaaga accaagttgt ccaaccaatg gaccattgtt gaggtaggcg gtcaagaatc
     3721 tcaggggaac cgcaagtcaa atctgagctc gagcctgtcc ggaaaacgaa ggtctatgaa
     3781 ggagtcgccg ggcagattca gcgtttgatt cgcgacggcc ggctcaatcc gggcgacaaa
     3841 cttccgcccg agcgtgagct ggccgaggca tttgcggtca gccgaacctc ggttcgggac
     3901 gcgatccgcg ttctcgagat gcagggcatg gtcgaacctc gccagggcga cgggaccgtt
     3961 gtccgagacc tctcgccgga ttctctcgtc aacccgctta cgtcgctact ggcgcgtaac
     4021 ggccgtctgt tgaccgaact cctcgatgtc cgcaagatgc tggaaccgcc gcttgccgct
     4081 agagccgcga cacatgcgtc cgaagtggat gttaacaagc tgcgcgcgat tctggatcgc
     4141 caggcggaga aggttcagcg gggcgagctc gctatcgatg aagacaccca gtttcactac
     4201 atgattgcca ccgcggcgca gaacagcgtt gttatgcgct tggtggacgt gctcatggat
     4261 ctgcttcgag agagccggga gcggtcgctg caggtgaaag gccgtttgca gaagtcgctg
     4321 gccggtcacc ggcagatttt tgcggccatc cattcgcgtg atcaggcggc cgccgaagcg
     4381 gcgatgcgcc agcacttgca ggagatcgaa gacgtgattg ttgggaggag ctaaatatgg
     4441 gccggatgtt tgcagtcgag atcgtttatc gcgggatctt tcaaaagaat ctcgcaaaaa
     4501 atatcagtcg cggcattgcg ctggctgcca agcacgacgg aaaaaccgcc atctcattcg
     4561 gccggtacgg cgacagcccg gagcgcaacg gcatccctgc gaagaacttc gccatcgtcg
     4621 ccaccgacgg ggaaacgctc gaggaaggca tggcgaaata cgagcccaag gaagtcgaca
     4681 tttccattgc ggtcgacgac acgctgtgta aaggcgtcga gtcgtgggcc tggtccggac
     4741 ttcagccgat caacaggctg ctcaagcccg gcggcacgtt gatcgtcacc tcgatgcacg
     4801 agcccgaggt cctcatcgac atggcccacc gaaaggaaac gccctacaac ctggcgatcg
     4861 tcaagagcat ccccagcttc gccgggttgt gggtctacaa ggatgaccac accgacgtgc
     4921 gcatcctggg cgccatcgcc aaggtgctgc ccgacctgtt cagcattgag tcggtggaga
     4981 aggcgatcct ggaagagtgg cacgaaccgc tgaaggcaac ttcggcgcgc aaatcgtacg
     5041 agcgcatcac cactcgtccc gtccagccca ctgagggcag cgcagagaag ccatacgagt
     5101 tcgagctgcc aaagtggtgg gagatgcgcg aggggctggc catcccgagc atccatatgg
     5161 gcggggaaat caccgacccg actacccagg tgtccggcgg gtaccgtccc gatcgcaatc
     5221 caacctttaa gaagtactcc acgcgcacga tgcggccggt tgtggatttc gacacttgcg
     5281 tgaaatgcac gctgtgctgg ctgcagtgcc cggactcctg ctttgacgtc acccccgacg
     5341 gcttatacga cgcgaacatg caggcatgct gtggttgcgg cgtctgcgag gcggtgtgtc
     5401 cggtggacaa ctgcgtcacg atggtcaacg agtcggcgtt tcccgataac gccagccagt
     5461 gggagatgta tcggcaggac aaaccgggct acaagacctg gctccaggaa aagatccagc
     5521 acaagcccga ccgctcccac ggcttcaaca tccgcgggca gtatgagcgg cagattgaca
     5581 ccatgatcgc cgacgggacc atcgagggcg gcaaagaggc gatcaccgag aaggtcaaca
     5641 ccgatgtcgg ggaagccagc gcaggggggc ttgactaatg gcacaggtcg tcgagccggc
     5701 tctaaaggaa actgaatcac tcatcagcgg cagcgaggcg attgccgtcg cctgcaaact
     5761 cg
//
LOCUS       DBNGSB010000045         5064 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3863444, whole genome shotgun sequence.
ACCESSION   DBNGSB010000045 DBNGSB010000000
VERSION     DBNGSB010000045.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 5064)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5064)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5064
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3863444"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="AC159B_01305"
     CDS             complement(<1..197)
                     /locus_tag="AC159B_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376424.1"
                     /translation="MALVFTFPCGSCGRRYSVYYPKALLYELSGTAPRQMGEKEDEEE
                     RKSGAVESARQRAEAAGNIWA"
     gene            369..797
                     /locus_tag="AC159B_01310"
     CDS             369..797
                     /locus_tag="AC159B_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376425.1"
                     /translation="MERSWLKVVLMVSGLAAFGFVMYLLGFDAGAQNVAAQTPRPIAA
                     TPAQSPAPFQEVIPLPGPDNQQGQGQGECQPMILFYFQGQLFQLMPGPQDQQGQPGSP
                     PEFFPLQPYRGPAIPGLPFGPQSPQRITPPPGFQPVQPRF"
     gene            complement(896..1102)
                     /locus_tag="AC159B_01315"
     CDS             complement(896..1102)
                     /locus_tag="AC159B_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013301410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR3376426.1"
                     /translation="MQGTIRWFDARRGFGFIIPEEGGPDVFLYRTVLRAAGLKRLDPG
                     QRVDYEIEETPRGRKVTKIKLQQV"
     gene            1372..3840
                     /locus_tag="AC159B_01320"
     CDS             1372..3840
                     /locus_tag="AC159B_01320"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HIR3376427.1"
                     /translation="MNQQIDLRTRHRLAPEQLRWTCDPSLFEFQTTAELKADEVIVGQ
                     DRAVRALDLGLTMDQPGYHIYVTGPVGTGRATYTRKQVQAVAVTMPAPPDWCYVYNFE
                     QPDQPVALSLSPGTGGTFRKEMAELVDELKDAIRKLLASETFEARRTELLQSFEGRIN
                     EVWRELESKAAQLGFSIQRTPAGIATTPVGPSGEPISQDQFAAIPEAQREEIAKRGRE
                     LNEEVGEALRKVRAVEREAREALRELERRAVRSAAGHPVARLKERYTGHPKITEYLDK
                     LLTDVIEHLDDFKEQEEPQIPLQLPTLGRRPEPFQRYRVNMVVDNSHLSGAPMIFETN
                     PTYYNLLGKVEYRGEFGALVTDFTMIKSGVLQQANGGFLILQVKDLLTNPLSWEGLKR
                     ALRSQEVRIENIGEQLGLVPTSTLRPEPIPLRVKVVLIGTPYIFQLLYMLDEDFRKLF
                     KIKADFDTEVDRTDQTAALYATAIGAICNRQGLRPFDRTAVAKVLEQSARLADHQQRL
                     STRFNEVVEIIYEASAWAERLGHDVVSAVDVVKAIDEKVYRSSRVEERLRQMIAQGQL
                     LVDVEGRRAGQVNGLSVLQLGDYAFGHPSRITARTYVGSRGVVNIERETEMSGRLHSK
                     GVAILAHYLGGKYAQDRPLSLNASITFEQTYTDVEGDSASSTELFALLSELADLPVEQ
                     GIAVTGSINQKGEIQPIGGVNEKIEGYFAVCKVQGFNGTQGVVIPAQNTVNLMLSEDV
                     VQAVRSGDFQIWAVRTVDEGLEVLTGVPSGEANSSGRYPEGTTNYRVSRRLTELAERL
                     RRFTPAPRGGDGKGEDKADKDQKK"
     gene            3916..>5064
                     /locus_tag="AC159B_01325"
     CDS             3916..>5064
                     /locus_tag="AC159B_01325"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR3376428.1"
                     /translation="MMRVYLGGLLCAILILGTGVGPAFTQAAQTVSLEEAIATALQRH
                     PTLAASTKSLEAAQARLVQARAGNAVQVGLNGSVALGTLSATGSPTSSGASVTHNVSV
                     GASLPLFDGGERAAQITQAEAGVVSAQAALEATRQDLALDAADAYFAVLRAMRVVEVR
                     EGALQSARRQVEQAEALVRAGTAARADVIRAQSVAASADADLITARGDVELSYASLRS
                     AMGIPITQPIAVREPPEILVADVPPAEAAAEAVRGRPEIRRVQSEVASSQAALRIAEI
                     RSGLNVSADANSSVQVSTNPGQIGCSISTSVSLPIADGGRAKAAVDEARANLDAARAR
                     AEATAQQIQLQAFQAALIIQQTRARLEAVRVAAAAAEESLRVAEGRYRA"
BASE COUNT         1012 a         1519 c         1590 g          943 t
ORIGIN      
        1 tcggcccaga tgttgcccgc ggcctcggcg cgctgtcgcg ccgactcaac cgctccggac
       61 ttgcgctctt cttcgtcttc cttttcaccc atctgccgcg gcgccgtccc gctgagctca
      121 tagaggagcg ccttgggata gtagacgctg tatcgtctcc cacaactgcc gcaggggaaa
      181 gtgaagacta gagccataaa ctaattgtaa ggcaagttgg tctggtagaa tacggagtac
      241 cggcgccctt cggctcaccg ggttcgctca gggcattcga ctcggcgcgg ccgaaggcca
      301 tgagcgagcc tgtaaagcag gcgagtcgaa tgggaacgcg accggcaagg ctttaggagg
      361 aacgggcgat ggagcggtca tggctgaagg ttgtcttgat ggtttcgggt ctcgcggcgt
      421 tcggtttcgt gatgtatctg ctgggatttg atgccggcgc gcagaacgtt gccgcgcaga
      481 cgcccaggcc gatcgcggct actccggcgc agagccccgc cccgttccag gaagtgatcc
      541 cgctaccggg cccggacaac caacagggtc aggggcaggg agaatgtcag cccatgatcc
      601 tgttctactt tcagggccag ttgttccagc tgatgccggg accccaagat caacaggggc
      661 aaccgggcag cccgcctgag ttcttcccgc tccaacccta ccgaggaccg gccatccctg
      721 ggctgccgtt tgggccgcag tctccacagc ggatcacccc gccgccggga tttcaaccgg
      781 tgcagccacg gttttagaag aaaccagcag tcattgcgag cgaagcgaag caatctctta
      841 tagaccggag attgcttcgt cgcttctagc tcctcgcaat gacgacgaac cgaacctaca
      901 cttgctgaag cttgattttc gttacctttc ggcctcgcgg cgtctcctca atctcataat
      961 cgacgcgctg acctggatca agacgcttta atccggcagc ccgaagcact gtccggtaga
     1021 gaaagacgtc gggtccgccc tcttcgggaa tgatgaatcc gaatccgcgg cgagcgtcga
     1081 accagcgtat cgtcccttgc atttcaagct tgtcccctta cactttccga acaaaagttc
     1141 ctgattgacg ctccaagact gcagatgtat ggtgagtttt gctagcggat gcgtattacc
     1201 tagctgggag gaagttaacc tccctaggtt aacccagcct aactgggtaa cgatccgcta
     1261 gcttttttgc tccccctccc cccaattgtg ctgctatcat tcatttgcga tgctcggact
     1321 cggccaaacc ggtccggtgc atgtgacatt cctcaataga atgattcaaa tatgaaccag
     1381 cagattgatt tgcgcacgcg ccaccggctc gcgcccgaac agttgcggtg gacgtgcgat
     1441 cccagtcttt tcgagtttca gaccaccgct gaactcaaag cggacgaggt tatcgtgggg
     1501 caggaccggg ccgtccgcgc gcttgatctc ggcctcacga tggatcagcc cggttaccac
     1561 atctacgtca ccggcccggt gggcacaggg cgggcgacgt acacgagaaa acaggtgcag
     1621 gccgttgcgg tgacgatgcc tgcgccgcct gattggtgtt acgtctataa tttcgagcag
     1681 cccgatcagc cggtcgcgct atcgctgtcg cccggtaccg ggggaacatt ccgcaaagag
     1741 atggcggagc ttgtcgatga gctcaaggac gccatccgca aattgctggc cagcgagacg
     1801 tttgaggccc gccgcaccga actgctccag tccttcgaag gccgcatcaa cgaagtctgg
     1861 cgcgagctgg aatcgaaagc ggcgcagctg gggttttcaa tccagcgaac tccggccggg
     1921 atagccacga cccccgttgg tccttcaggt gaacccatca gccaggatca gtttgcggcc
     1981 attcccgaag cgcaacgcga ggagatcgcc aagcgggggc gcgagctcaa tgaggaggtc
     2041 ggcgaggcgc tccgcaaggt gcgtgccgtt gagcgagaag cgcgcgaggc gctgcgcgag
     2101 ctggaacgcc gggccgttcg atcggcggcg ggccatcccg ttgcccgtct caaagagcgg
     2161 tacaccggac atcctaagat taccgagtac ctcgacaagc tcctgaccga cgtcatcgag
     2221 cacctcgacg atttcaaaga acaggaagag ccgcagatcc ccctgcaact gccaaccctc
     2281 ggccgccgtc ccgagccgtt ccagcgatat cgcgtgaaca tggtggtgga taacagccac
     2341 ctctcggggg cgccgatgat ttttgaaacc aatcccacct attacaacct cctcggcaag
     2401 gtggagtacc gcggtgagtt tggggcgctc gtcaccgact tcacgatgat caagagcggc
     2461 gtgcttcagc aggccaacgg tggatttctc atccttcaag tgaaggactt gctgaccaat
     2521 ccgctctcct gggaaggcct caagcgcgcg ctccgcagcc aggaggtgcg tattgagaac
     2581 atcggcgagc agctgggact tgttcccaca tcgacgctgc gtccggagcc gattccgctc
     2641 agggtgaagg tcgtgctgat cggcaccccc tacatatttc aactcctgta tatgctggat
     2701 gaggattttc gaaagctctt caagatcaaa gcagactttg acaccgaggt cgatcgcacg
     2761 gaccagaccg cggcgttgta tgcgacggct atcggcgcaa tctgcaaccg gcagggactg
     2821 cgtccgttcg atcggactgc cgttgcgaaa gtcctggaac aaagcgcgag actcgccgac
     2881 caccaacagc gtttatccac tcggttcaat gaagtcgtgg agatcatcta cgaggcctca
     2941 gcgtgggcgg aacgcttggg ccatgacgtc gtcagcgccg tggatgtcgt caaagcgatc
     3001 gatgagaagg tgtaccggtc cagccgcgtc gaggagcggc tgcggcagat gatcgcgcag
     3061 ggacagcttc tcgtcgacgt ggaaggccgc cgcgcaggac aggtcaacgg cctgtccgtt
     3121 ctgcagctgg gtgactacgc cttcggccat ccgagccgca tcaccgcccg cacgtacgtt
     3181 ggcagccgcg gcgtggtgaa catcgagcga gaaaccgaaa tgtcggggcg cctccacagt
     3241 aaaggcgtgg ccatccttgc gcactacctc ggggggaagt acgcgcagga tcgtcctctg
     3301 agcctgaacg cgtccatcac gttcgagcaa acctatacag atgtggaagg cgacagcgca
     3361 agctcgacgg agctgttcgc gttgctctcg gagttggctg atctcccagt cgagcagggg
     3421 atcgccgtga cgggatcgat caatcagaaa ggcgagattc agccgatcgg cggtgtaaac
     3481 gagaaaatcg aaggatattt cgccgtatgc aaggtccagg gattcaacgg cacgcaagga
     3541 gtcgtgatcc ctgctcagaa cactgtgaac ctcatgctga gcgaagacgt ggtgcaggcg
     3601 gtccgttccg gagactttca gatctgggcc gtgcgtacgg tagacgaagg cctcgaggtg
     3661 ctgaccggtg tgccctctgg tgaggcgaac tcctccggca ggtatccgga aggcacgacc
     3721 aactaccggg tgagccggag actgacggaa cttgccgaac gcttgcgacg ttttacacct
     3781 gccccccgcg gcggcgatgg gaagggcgag gacaaggcgg ataaggacca aaagaagtaa
     3841 acgtcgatta ctgcgtggtc gtcaaattac aagataacta cggggggttt cccgtagttt
     3901 ctgtttaggg gaggcatgat gcgggtctat ctcggggggc tcctgtgcgc aatcttaatc
     3961 cttggaaccg gagttggccc ggccttcact caagccgcgc aaaccgtgtc gctggaagag
     4021 gccatagcca cggcactgca gcgacatccc acgttggccg cttcaacgaa atcgctcgag
     4081 gcggcgcagg cgcggcttgt gcaggcgcgg gcgggcaacg cggtgcaggt cgggctcaat
     4141 ggcagcgtgg cgctgggcac actgtccgca actgggagtc ccacgagtag tggtgcatcg
     4201 gtcacccaca atgtttccgt cggcgcttca cttccgcttt tcgacggggg cgagcgcgct
     4261 gctcagatca cgcaggcgga agcgggggtc gtgtccgctc aagcggcgtt ggaggcaacc
     4321 cgtcaggatc ttgcgctgga cgcggcggat gcctactttg cggtgctgcg cgcgatgcgc
     4381 gtcgtcgagg tgcgtgaagg cgcgctgcag agcgcgcgcc gccaggtgga gcaggcagaa
     4441 gcgctggtgc gggccggcac ggctgcacgc gcagacgtga tcagagcgca gtcggttgcc
     4501 gccagcgcgg acgccgatct gatcaccgcg agaggcgacg tcgagctttc ctatgcctcg
     4561 ctgcggagcg cgatgggaat tcccatcacg cagccgattg ccgtccgcga gcctccggag
     4621 atcctggtcg cggatgttcc gccggcggaa gcagcggccg aggccgtccg cgggcgtccg
     4681 gaaatccgcc gggtacagtc cgaagtcgca agttcgcagg cggctctccg cattgctgag
     4741 atccgctcag gcctcaacgt ctctgctgat gcgaacagct ccgtccaggt gtcgacgaac
     4801 cccggccaga ttgggtgttc gatatcgaca agcgtgtcac ttccgattgc ggatggaggg
     4861 cgggcaaaag ccgccgttga cgaggccaga gccaacctcg atgccgctcg ggccagagcc
     4921 gaagcgaccg cgcagcagat ccagctccag gcgtttcaag ccgcgttgat catccagcag
     4981 acacgagcgc gcctcgaagc ggtcagggtc gcagccgcgg cagcggagga atcgctgcga
     5041 gtggccgagg gtcgctatcg cgcc
//
LOCUS       DBNGSB010000046         5378 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3864375, whole genome shotgun sequence.
ACCESSION   DBNGSB010000046 DBNGSB010000000
VERSION     DBNGSB010000046.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 5378)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5378)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5378
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3864375"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="AC159B_01330"
     CDS             complement(<1..990)
                     /locus_tag="AC159B_01330"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3376429.1"
                     /translation="MRVLRPPLRARLPVQLLLPAGIAAIVAYLTRLPLIFLLYGTFFT
                     PTVGGQAGTFTLQNYLRAYTDRSALPIMANSFVFAVGTMVVSLVIGTMLAWITERTNT
                     PLRGLFFAVSLVPLIIPGILFTLSWIFLLSPRIGLLNLWIMRLLGLSAPPFDIYSIGG
                     MIWVEALHWSPIAYLMMSAAFRSMDPSLEEAALMSGSGLARTFYHVTLKLNVPTLLGA
                     SLLLFLRGLETFEVPAIIGLPAGIHVFTSRIYLAIKSYPSDFGLAGSYSTILLVMTLT
                     GIFLYGRITREASRFATITGKGFRPHVIDLGIWKYAMAALIVGYLVVMVVLPMF"
     gene            complement(1027..2103)
                     /locus_tag="AC159B_01335"
     CDS             complement(1027..2103)
                     /locus_tag="AC159B_01335"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3376430.1"
                     /translation="MRKGRMRMWTAAALVLVWTLVSVQAGAAPSGSIAAIAMYKGPDR
                     QARLEAGARQEGALMIYTSMDLEESQPILEVFMKKYPYIRGEVYRAPGEDVAQKLITE
                     YRGRKYLADVFEGTGIDVAKMIKETYGQAFFTPRAGAYPRQAKDPKGFWVATRYNILV
                     AAWNTNVVSDADSPRTYEDLINPKWRGRIGIEATDQVWLGTLMELWGEAKAMEFFRQL
                     SAQQPLIRTGHTLLAELIVAGEVPMSPTIYNHRSERLKSRRAPIDWRPLQPAVAIPHV
                     IGLPNRAPHPHAALLFIDFLLSREGQNELVKLGRIPAYPLVNAAPPYLNQGFAYKVID
                     PSTFLDKYQRYDALWQDMIIRARR"
     gene            complement(2100..2582)
                     /locus_tag="AC159B_01340"
     CDS             complement(2100..2582)
                     /locus_tag="AC159B_01340"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3376431.1"
                     /translation="MERGTIDETSEREEFRKKHLYEGTSFKNLRSHPVHQRKMRGSSS
                     AHFALSGNKTIDAHISELPPGGQNKRHRHMNEAIIYILSGRGYSIIQNGDETPVRIDW
                     QEGDMFSPPLWWWHQHFNGDPDRPARYLAVTNAPLMITIGLFKKEQAVDQSKGAGEKR
                     "
     gene            complement(2684..3832)
                     /locus_tag="AC159B_01345"
     CDS             complement(2684..3832)
                     /locus_tag="AC159B_01345"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR3376432.1"
                     /translation="MRGTGNRERGTVTEAGLPTVEAAVAVAPLRTEMQRLPIPTIGED
                     DGLLAVEACGLCGTDWDFYTRKRGAKLGPIILGHEIVGRVIDVGPQAAQRWKVKPGDR
                     VAVEEFLPCGQCEFCLSGRPAMCEATDSRSEHGFLRYGATPTALPPGLWGGFSEILYL
                     HPRALVHRLADDITSDLATLFVPISNGVRWMLKEGRLRRGGTAVIIGPGAHGLGCVIA
                     ALEGGASHVFAVGRSRSVRLEAAAALGAIPIESERANVIETVRQKTNGAMADVIVDLA
                     PDSVETVETAVALARKGGTVLLASSKHGRPIAGFQSDTVVRNELTVKGVRGRDYESVE
                     DALRIIRSGKYPLALLRTHRFSLGQTDQALRVLGERTDPSAIHVAVMP"
     gene            complement(3829..4290)
                     /locus_tag="AC159B_01350"
     CDS             complement(3829..4290)
                     /locus_tag="AC159B_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376433.1"
                     /translation="MKTKVTDTPLARVGNVEVSKFETSTGARLRLRRAGSAGAEAYLD
                     PLELEGLTRLRSKPIAVLGPAGAGREHAVRPAGAEPVEHLQNEFAMVGVSVSRSNRDA
                     GLIIRDMNAGQTVLLTPQELEALLVARHMDFAPLIDTSDLVAIPEPDIDQA"
     gene            complement(4297..4806)
                     /locus_tag="AC159B_01355"
     CDS             complement(4297..4806)
                     /locus_tag="AC159B_01355"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HIR3376434.1"
                     /translation="MTPRASLGVEEAQRFLFAEARLLDERRFDEWEGLFTDDGEYWLP
                     IDPERDPAGAISIIRDDRKRLQERVYRLTKTPVLDQNPHSRTVHFVSNVEVEPAQSNG
                     ENLVRCHQLIAEMRPGGLGQAGLNAPRWIAARCEYRLRNVDGAWKIAQKKVVFLNSDE
                     PHYNLSFIP"
     gene            complement(4778..>5378)
                     /locus_tag="AC159B_01360"
     CDS             complement(4778..>5378)
                     /locus_tag="AC159B_01360"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3376435.1"
                     /translation="ESTVDEVQRYRQEGCARGMVNGHGLLERPGSLNPAASWTARMMD
                     RYPEYAGAMKQRFSEQELVEISARRNIFVFPNLYLFDTHIRVIVPKASDKTEVQLYVY
                     ALKGIPDKINEGRYRGHERFYGPAGFGAPDDIEVFTEVQTGVHAKGNVWNLINRGQHR
                     ERRENGEWVGHTTDEAPQRSLYRHWRQVMEDHPESIPGR"
BASE COUNT         1006 a         1707 c         1613 g         1052 t
ORIGIN      
        1 gaacatcggc aggaccacca taaccacgag atacccaacg atcaacgccg ccatggcata
       61 tttccagatc cctagatcga tgacatgcgg cctaaaccct ttgccggtga tcgtggcaaa
      121 tcgcgacgcc tctcgtgtaa tccggccata caagaagatg ccggtcaatg tcatcaccag
      181 cagaatcgtc gagtatgatc ccgccaggcc gaagtctgag ggatagctct tgatcgcaag
      241 atagatcctg gaggtgaata cgtggatgcc cgccggcaat ccgatgatcg ccggcacttc
      301 gaacgtctcc aggccgcgca ggaagagcaa cagactggcg cccaggagcg tcggcacgtt
      361 caactttagc gtcacgtgat agaaggtgcg tgccagaccg ctcccggaca tcaacgcggc
      421 ttcttcgagt gacggatcca tcgagcgaaa tgcggcagac atcatcaagt acgcgatcgg
      481 agaccaatgc agggcttcaa cccagatcat cccgccgatc gaatagatgt cgaatggcgg
      541 cgccgagaga cccagaagcc gcatgatcca caggttcagc agcccgatcc gcgggctcag
      601 caggaagatc caactcagcg taaacaggat gcctgggatg atcagcggga cgaggctgac
      661 ggcaaagaac aatccccgca gcggcgtgtt ggtgcgctcc gtgatccagg ccagcatcgt
      721 accgatcacc agcgaaacca ccatcgtgcc cacggcgaac acaaaggaat tcgccatgat
      781 cgggagcgct gagcggtcgg tgtacgcgcg gaggtagttt tgcagcgtga acgtaccggc
      841 ctggcccccg acggtgggcg tgaagaaagt gccatacagc agaaagatca ggggcagccg
      901 cgtcaggtag gcaacgatcg cggcgatccc agccggcaac agcaactgca caggcagccg
      961 cgcccgcagc ggcgggcgca agaccctcac cgtggcgggc gcggtgccgt ccaatgcgtt
     1021 ctgttcctag cgtcgggcgc ggatgatcat gtcctgccag agggcatcgt atcgctggta
     1081 cttgtccagg aacgtcgacg ggtcaatcac cttgtaggca aacccctggt tgagatacgg
     1141 cggcgcggca tttaccaacg ggtaggcagg gatgcgaccg agcttgacta actcattctg
     1201 cccctctctc gacagaagaa agtctatgaa cagcaatgct gcatggggat gcggcgcgcg
     1261 attgggcagc ccgatcacat gcggaatggc gacggccggc tgcagcgggc gccagtcgat
     1321 tggggcgcgc cgtgacttga gacgctcgga acggtgattg tagattgtcg gcgacatcgg
     1381 cacctctccc gcgacgatca gttccgcgag gagtgtgtgg cccgtccgga tcagcggctg
     1441 ctgcgcgctc agctggcgga agaactccat cgccttggct tcgccccaca gttccatcag
     1501 cgtccctagc cagacctggt cggtcgcctc gatgccaatg cggccccgcc acttgggatt
     1561 gatcaaatct tcgtaagttc gcggagaatc agcgtcggag acgacgttgg tgttccaggc
     1621 cgcgacaaga atgttgtagc gcgtggcgac ccagaaccct ttcgggtcct tagcctgccg
     1681 cggataggcg ccggcgcgcg gcgtgaagaa tgcctgcccg taggtttcct tgatcatctt
     1741 ggcaacgtcg atccccgtgc cctcgaacac gtcggccagg tacttacgcc ctcgatactc
     1801 cgtgataagt ttttgtgcga cgtcttcgcc tggagctcga tagacctcgc ctcgaatgta
     1861 tggatatttc ttcatgaata cctcaaggat cggctgggac tcctcgaggt ccatcgaagt
     1921 gtaaatcatc agcgcgcctt cctggcgcgc gccggcttcc agcctcgcct gccggtcggg
     1981 ccctttgtac atggcgatgg cggctatgga tcccgatgga gccgcgccgg cctgtaccga
     2041 cacgagcgtc cacaccagta ccagcgccgc cgccgtccac attcgcatgc ggcctttcct
     2101 catcgcttct caccagctcc tttcgattga tcaactgcct gctctttctt gaacagtcct
     2161 atagttatca tgaggggcgc attggtcaca gccaggtaac gcgccgggcg gtccgggtcg
     2221 ccattgaagt gctgatgcca ccaccatagc ggcggcgaga acatgtcgcc ctcttgccag
     2281 tcgatccgca cgggcgtctc gtctccattt tggatgatcg agtagcctcg tccggagagg
     2341 atatagatga tcgcctcgtt catgtggcgg tgccgcttgt tctggccacc cggaggcagt
     2401 tcgctgatgt gcgcgtcgat cgtcttgttt ccagacaatg cgaagtgcgc cgaagaactc
     2461 ccccgcatct tccgctggtg caccgggtga ctgcggagat tcttgaacga tgttccttca
     2521 tacaagtgct tcttccgaaa ctcttcgcgc tcgctggttt catcgatggt gcctcgttcc
     2581 accggctgca cctccaccga ctcaaatagc cttttctatc attcgcgttg gtagaaagcg
     2641 tgtttcgggg gaggcggcgc cgctacctac tgtcccgccg ccgctagggc atgaccgcga
     2701 cgtggatcgc cgacgggtcg gtgcgctcac cgagtactct cagtgcctgg tcggtttgtc
     2761 ccaacgaaaa ccggtgggtg cgcaacagtg ccagaggata cttccctgag cggatgatcc
     2821 tcagcgcgtc ctcgaccgac tcgtagtcgc ggccgcggac gcctttcaca gtcaattcat
     2881 tgcggacaac cgtgtcgctt tgaaaaccgg cgatcggcct gccgtgtttg gaagacgcaa
     2941 ggagtaccgt cccgcctttt cgcgccagcg caacggctgt ttctaccgtc tcgaccgagt
     3001 ccggggccag atcgacgatc acatcggcca tggcgccatt cgtcttctgg cgaactgtct
     3061 cgatcacgtt ggcgcgctca gattcgatcg ggatcgcgcc cagggcggcc gcggcctcca
     3121 gccgcacact tcgcgagcgg cccacggcga agacgtgcga cgctccccct tcgagcgccg
     3181 caatgacaca gccgagcccg tgcgcccccg gtccaatgat gacggcggtg ccgccgcggc
     3241 gaagccgccc ttctttgagc atccatcgca cgccattcga gatcggaaca aatagcgtgg
     3301 caagatccga ggtaatgtcg tcggcaagtc ggtgcaccag cgctcgcgga tgcaggtaga
     3361 ggatctcact gaatccaccc cagagcccgg gtggaagggc cgtcggcgtc gcgccgtacc
     3421 ggagaaagcc atgctccgat ctcgaatccg tcgcctcgca catcgccggg cggccggaga
     3481 gacagaactc gcactggccg cacgggagaa attcctcgac ggccacgcgg tcgccgggtt
     3541 tcaccttcca acgctgcgct gcctgtggtc caacatcgat gacgcggccg acgatttcgt
     3601 gccccaggat gatcgggcca agtttggcgc cccgctttcg tgtgtagaag tcccagtcgg
     3661 tgccgcacag cccacaggcc tcgacggcga gcagcccgtc gtcctcaccg attgtgggaa
     3721 tgggcaggcg ctgcatctcc gtgcgcaatg gcgccacggc caccgcggct tcgacagtcg
     3781 ggagaccagc ttcagtgacc gttccccgtt cccggttccc cgttcccctc atgcctggtc
     3841 gatgtctggc tcggggatcg caaccaggtc gctggtgtca atcagcggag cgaagtccat
     3901 gtggcgggcc accagcagtg cctcgagttc ctgcggggtg aggagcaccg tctggccggc
     3961 attcatatct cgaatgatga gacctgcatc cctgtttgag cggcttacgc tcacgccgac
     4021 catcgcgaac tcgttttgca gatgctcaac gggttccgcc ccggccggtc gaacggcgtg
     4081 ctcgcggccc gctccggcgg ggccaagcac cgcgatcggc ttggagcgca gacgggtgag
     4141 tccctcgagc tcgagcggat cgagataggc ttcggcccct gcggatccgg cgcgccgcaa
     4201 acgcagacga gcgcccgtgg acgtttcgaa cttgctgact tccacgttgc cgactcgcgc
     4261 gaggggagta tctgtgacct tagtcttcat gcgtcactag ggaatgaacg acaggttgta
     4321 atgaggttca tccgaattca ggaagaccac tttcttctgc gcgatcttcc acgcaccgtc
     4381 gacgttgcgg agacggtact cacaccgcgc cgcgatccag cgcggagcat tcaaccccgc
     4441 ctgtcccagg ccacccggcc gcatctcggc gatgagctgg tgacagcgga cgaggttctc
     4501 tccgttggac tgcgccggct cgacttcaac atttgagacg aaatgcacgg tgcgtgaatg
     4561 gggattctgg tccagcaccg gagtcttggt caaacgatag acgcgttctt gcagacgctt
     4621 gcggtcgtcg cggatgatcg agatagcccc ggccggatcg cgctccgggt cgatcggcag
     4681 ccagtactcg ccgtcgtcag tgaacagccc ttcccattcg tcgaaccggc gctcatcgag
     4741 cagccgcgct tcggcgaaca ggaagcgctg cgcttcttca acgcccaggg atgctctcgg
     4801 ggtgatcttc catcacctga cgccagtggc gatacaacga gcgttgaggc gcttcgtccg
     4861 tcgtgtggcc tacccactcg ccattctctc tgcgctcccg gtgctgaccg cggttgatca
     4921 ggttccacac attccccttc gcgtgcaccc cggtctggac ttcggtgaac acttcgatgt
     4981 cgtcgggcgc gccgaaccct gccggtccat agaatcgctc gtgccctcgg tacctgccct
     5041 cgttgatctt gtcggggatg cctttgagtg cgtatacata cagctggacc tcagttttat
     5101 cagaggcctt cgggacgatc acgcggatgt gcgtatcgaa gaggtagagg ttcgggaaga
     5161 caaagatgtt tctgcgcgcc gagatctcga cgagctcttg ttcggaaaag cgctgcttca
     5221 tggcccctgc atactccgga tagcggtcca tcatcctcgc ggtccagctc gcggcgggat
     5281 tgagactgcc tggccgctcc agcaacccgt gcccgttgac catgccgcgg gcgcatcctt
     5341 cctggcggta ccgctgcacc tcatcgaccg ttgattct
//
LOCUS       DBNGSB010000047         3056 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_1105256, whole genome shotgun sequence.
ACCESSION   DBNGSB010000047 DBNGSB010000000
VERSION     DBNGSB010000047.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_1105256"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..1284
                     /locus_tag="AC159B_01365"
     CDS             190..1284
                     /locus_tag="AC159B_01365"
                     /inference="COORDINATES: protein motif:HMM:NF013847.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen
                     decarboxylase/cobalamine-independent methonine synthase
                     family protein"
                     /protein_id="HIR3376436.1"
                     /translation="MTVSYRADHVGSLLRPKELLDARSAPDPDPERLKKLEDQHILRV
                     LDRQHDLGFEIFTDGELRRRNFMSDLFDSVEGIDTESAVGRTWQGGTAVAVSSVTGIA
                     TGRIRQIRRLTAHELTFLKANSPGAIKITLPSANQFPAIAYKRGITDQVYRNHSELLW
                     DIVPIVKSEIQALADEGVRYIQLDAPRYSYYIDPKWRDYVRREMGVEPDEALDEALRA
                     DNTCFEGVRRLGLTLAIHLCRGNNRSQWYAEGGYDPIAEKLFNSLQVDRFLLEYDDQR
                     SGTFEPLRFVPPGKTVVLGLVSSKKPVLESKRDLFSRIKEAERHVPLEHLALSPQCGF
                     ASTMEGNLVTEDDQWAKLKLVAETAREVWT"
     gene            1422..2621
                     /locus_tag="AC159B_01370"
     CDS             1422..2621
                     /locus_tag="AC159B_01370"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3376437.1"
                     /translation="MRRVPLLVLIVGVSVSLLAQGGAAAPSGTIRLGAVVPLTGRFAS
                     GGAQVKAGYEIAVEDINARGGVQVGNRRMPLELVMLDDESDPTKTVSRLETLATQGVV
                     TYLGGFGSDLHAAAAAVAEKNRIPYCGVAFALHAVHQQGFRFLFSPFIKSPDIAIGIY
                     RFFNSLPAAQRPRRIAIFSERTDWGRELDRLWTARSTENGYQVVLSAEYTVGTRDFSD
                     IILRAKAAGAEAVLSLPTPPDGLAIMRQMKELDFNPKAILMIRAPDAPSWTQALGKDG
                     DYVLLTPGWHYNFRAAGVTELNAKHQQRFGRPADVITGPSYACVQVVADAITRAGRLD
                     PPAIRDAIAATNLQTVVGTVRFRADGTGAQPFVVVQWQNGAQQLVWPKEMGAVPFLYP
                     ARPWRER"
     gene            2611..>3056
                     /locus_tag="AC159B_01375"
     CDS             2611..>3056
                     /locus_tag="AC159B_01375"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3376438.1"
                     /translation="MNGDGQNLLEAEDLSKSFGGLRAVDRLSFTVDEGEILGLLGPNG
                     SGKTTVFNLIAGALRPDRGRVRFSGRDITHEAPSVRTRLGIARTFQLVRPLSSLTALD
                     NVLVARLYGRGRSIMLREARNESLDLLYLFGLAEKAGMPAASLTLA"
BASE COUNT          572 a          935 c          972 g          577 t
ORIGIN      
        1 cgagatgccg gtcgcagagc aatctgatcg cctgaggcgt caacgggaac gtaaggttgg
       61 ctgggtcgag gacgatgtag tcgttaccga gcgcgtggga cttgacgaag ggattgcgct
      121 ccatcactga tgggtgtgtt cggacggcaa gagttgactt cctgtccggc gaatactgac
      181 caaactcaga tgaccgtctc ataccgcgcc gatcacgtcg gcagtttgct ccgccccaag
      241 gaacttctgg acgcgcgaag cgcgccggat cccgacccag agcgtcttaa gaaactcgaa
      301 gatcagcaca tcctgcgcgt gctcgacagg cagcacgatc tcggttttga gatttttacg
      361 gacggtgagt tgcgacgacg taacttcatg agtgatctct tcgattcagt cgaaggcatc
      421 gacaccgaaa gcgcagtagg acggacctgg cagggcggga cggccgtcgc cgtgagcagc
      481 gtaaccggca tcgccaccgg caggatccgt caaatccgcc gcctcaccgc gcacgagctc
      541 acgttcctga aagccaacag ccctggcgcg ataaagatca cgcttcccag cgccaaccag
      601 tttccggcga tcgcctacaa gcgcgggatc acggaccagg tctacaggaa ccactccgag
      661 cttctctggg atatcgtccc gatcgttaag tcggagattc aagcgttggc cgatgagggc
      721 gtgcggtaca ttcagctcga cgcgccgcgg tacagttact acatcgatcc gaagtggcgg
      781 gattatgtgc gccgcgagat gggcgtcgag ccggacgagg cgctggatga ggcgctccgg
      841 gcggataata cctgcttcga aggggtgcgc cggctcggtc ttacgctcgc gatccacctg
      901 tgtcgcggga acaaccgcag ccagtggtat gcggagggtg gatacgaccc gattgccgag
      961 aagttgttca actcgctcca ggtcgatcgt ttcctgttgg aatacgacga ccagcgttcc
     1021 ggaacattcg agcccctccg gttcgtcccg ccaggaaaga ccgtggtcct gggtcttgtg
     1081 agcagtaaga agcccgtcct cgaatccaag cgagatttgt ttagccggat caaagaagct
     1141 gagcggcacg tcccactcga gcaccttgca ttgagcccgc agtgcggctt tgcctcgacg
     1201 atggaaggca acctcgtcac cgaggatgat caatgggcaa agctcaagct tgtcgcggag
     1261 actgcgagag aggtgtggac gtaagtggcg cccacgagtc ttgcctgcgc agcaggccga
     1321 cggcgcacag aagccgagct gcagcgaggg tgggggattc ttgcttggtg ccgggttaag
     1381 taggacgcga ggtttgatat taacactcgg aggtggagtg catgagaaga gttcctctgc
     1441 tcgtcctgat tgtcggagtc tctgtcagcc tgcttgcgca gggtggtgcg gccgcaccgt
     1501 cggggacgat ccggcttggc gccgttgtcc ccctgaccgg ccgtttcgcc agcggcggag
     1561 ctcaagtcaa ggccggatac gagatcgctg ttgaggacat caacgcgcgc ggcggagtgc
     1621 aggtcggtaa ccgccgcatg ccgctggaac tcgtcatgct ggacgatgag tcggatccta
     1681 cgaagacggt cagccgtctg gagacgctgg caacacaagg tgtcgtgacc tacctgggag
     1741 gctttgggag cgacctccac gcggccgcgg ccgcggtggc ggagaagaac cgcatcccgt
     1801 actgcggcgt cgcgttcgcc ctccatgcgg ttcaccagca gggcttcagg tttctctttt
     1861 cgccattcat caagtctccg gatattgcga tcggcatcta tcggttcttc aacagtctgc
     1921 cggcggcaca gcgcccgcgg cgcatcgcga tcttctccga gcgcacagac tggggccggg
     1981 aactcgaccg gctgtggacg gcgcgctcaa cggagaatgg ctatcaggtg gtcctctccg
     2041 ccgagtacac ggtggggacg cgcgacttct ctgacatcat cctgcgcgcg aaagccgccg
     2101 gagctgaggc ggtcctctct ttgcccacgc cgccggacgg cctcgccatc atgcggcaga
     2161 tgaaagagct cgatttcaat cccaaggcca tcctcatgat cagggcgccc gacgcgccct
     2221 cttggacaca ggcgctgggc aaagacgggg actacgtgct cttgacgccc ggctggcatt
     2281 acaacttccg tgccgccggc gtgaccgagc tcaatgccaa gcaccagcag cgtttcgggc
     2341 gacccgccga cgtcatcacc gggccaagct acgcgtgcgt ccaggtcgtt gcggacgcga
     2401 tcactcgagc cggtcggttg gatccccccg cgatccgcga cgccatcgcg gcgacgaacc
     2461 tgcagaccgt ggtcggaacc gtccggttcc gcgccgacgg cacgggcgcg cagccgttcg
     2521 tcgtggtgca gtggcagaac ggggcacagc agcttgtgtg gcctaaggag atgggcgcgg
     2581 tccccttcct gtacccggcc cgcccctggc gtgaacggtg acggtcagaa tttgctcgag
     2641 gctgaggatc tttcgaagtc cttcggtgga ctgcgcgccg tagatcggct ctccttcacg
     2701 gtcgacgaag gagagatcct cggcctgctc ggccccaacg gatccggtaa gaccaccgtc
     2761 ttcaatctta tcgctggcgc gctgcgcccg gatcgtggcc gcgtgcgttt ctccggccgt
     2821 gacatcacgc acgaagctcc cagcgtcaga acacgcctcg ggattgcccg tacgtttcag
     2881 ctcgtgcgcc ccctttctag tctcacggcg ctggacaatg ttctggtcgc tcgactgtac
     2941 ggccgcggcc gatctataat gctgcgggaa gcccgcaacg aaagtctgga tctcctctat
     3001 ctctttggtc tggcagaaaa agcgggaatg cccgccgcat cgttgacgct ggccga
//
LOCUS       DBNGSB010000048         2957 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_1872176, whole genome shotgun sequence.
ACCESSION   DBNGSB010000048 DBNGSB010000000
VERSION     DBNGSB010000048.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_1872176"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="AC159B_01380"
     CDS             complement(<1..964)
                     /locus_tag="AC159B_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376439.1"
                     /translation="MPASRPASFLQPVIELGAYMGVLIVVLCSKQTKVEQVARQVART
                     PGARSLIVPIPDKWKHSKFPARTSADEFKDANAGRESDLSAKRNLGLLLARLQGWNKV
                     AFVDDDIMDTDNIARLAAELERHQVAGMLVRRHPDNSVVCHARRLAGIAQGVFVSGAV
                     LGVHCNSLPLSFFPDIYNEDWFFFANEAAARKLPCVGHARQAEYDPFAAPDRARQEEF
                     GDLLAEGLYAVFGEEDPSVPFYEQLDAATRTYWSRFIEARREVITETMTRLDRFMQPN
                     ADDGRVSSALNSLAAAENHLGTSPPDPCRDFLDAWRDDLDDWQRF"
     gene            complement(1102..1611)
                     /locus_tag="AC159B_01385"
     CDS             complement(1102..1611)
                     /locus_tag="AC159B_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376440.1"
                     /translation="MSDDLSRHVILLGGIGWNEVTRRFQSAISQVPITQTAVDDLKGG
                     DIFQVETRESAEPKLFYPDYADLGDGEELIADVGYLARLRNPFKIDRTLTICNGIFSR
                     GVFGAVRCLTDANVRSENEKYLADKFPDGEFAILLRVPVVTNETLSPDLQSPDSRLYE
                     WAPNVGARR"
     gene            1666..1932
                     /locus_tag="AC159B_01390"
     CDS             1666..1932
                     /locus_tag="AC159B_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376441.1"
                     /translation="MPIELDQRIAVLPHPCEVRVLYVPEWTLHVRWGVRADHDHRPGV
                     KLESASHFAVLRMKQLQQLGFQFSEPVKLAWRQLIFWYQVGFPL"
     gene            2260..2430
                     /locus_tag="AC159B_01395"
     CDS             2260..2430
                     /locus_tag="AC159B_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376442.1"
                     /translation="MPVVVRLRAAGWDVFYDASGLDDFDEIRETMRRALADSVLFVAR
                     FSDVYPLRRACA"
BASE COUNT          551 a          897 c          890 g          619 t
ORIGIN      
        1 agaacctctg ccaatcgtcg aggtcgtctc gccacgcatc gaggaagtcc ctgcaggggt
       61 cgggtgggct tgtgcctagg tgattttcag cggccgccag cgagttcaga gccgaggaca
      121 cacggccgtc atctgcattc ggctgcatga agcgatcgag cctcgtcatc gtttcggtta
      181 ttacctcacg ccgggcttcg atgaatcgcg accagtacgt cctcgtggct gcatcgagtt
      241 gctcgtagaa cggaacactc gggtcttctt caccgaagac ggcgtacagg ccttcagcca
      301 ataggtctcc gaattcctcc tgacgagctc gatccggggc cgcaaagggg tcgtactcag
      361 cctgcctggc atggcctaca catggcagct tgcgtgcggc ggcctcgttc gcaaagaaga
      421 accagtcttc gttgtatatg tccgggaaga atgacagcgg caggctgttg cagtgcacac
      481 ccaagaccgc accggacacg aagacccctt gggcaatgcc ggcgagccgg cgggcgtggc
      541 ataccacgga gttgtcgggg tgccggcgaa cgagcatccc cgccacttgg tgccgctcca
      601 attccgccgc gagacgcgcg atgttgtctg tgtccataat gtcgtcgtct acgaaggcga
      661 ccttgttcca cccctggagg cgcgccagca ggagtccgag gttgcgtttg gcgcttaagt
      721 cgctctcccg accagcgttc gcatccttaa actcatctgc cgaggttcgg gcggggaatt
      781 tggagtgctt ccacttatcg ggtatcggca cgatgagcga gcgtgcaccg ggcgtcctcg
      841 ccacctgcct tgccacctgc tcaaccttcg tttgcttact gcacagaacg actattagga
      901 cgcccatgta cgcgcccaat tcgatcaccg gctggaggaa ggacgcgggc ctggacgccg
      961 gcacaatgat tgcgtccagt cgcccccgcc gcgacctcgt cggtggcgcg ggcgatcgtg
     1021 acaagtcttg agatagatga gcgtgtgacg cccgctgctc gacccttagc gaccggatgg
     1081 atgccttagt tcctgcgccg ctcaccgccg agcacccacg ttcggtgccc attcgtaaag
     1141 tcgagaatcg gggctttgca ggtcgggtga caaggtctcg ttggtgacca ctggaactcg
     1201 cagcaagatg gcaaactctc cgtccggaaa cttatctgct aggtatttct cgttctcgct
     1261 ccggacattg gcatccgtca ggcaacgaac cgctccgaag acaccgcggc tgaatattcc
     1321 gttgcaaata gtgagagtgc ggtcgatctt gaaagggttg cgaagacgag caaggtagcc
     1381 gacgtcagca atgagttcct cgccgtcgcc aagatccgca taatccggat agaagagctt
     1441 cggctctgcg ctttcgcggg tctcaacttg gaatatgtct ccacccttca ggtcatccac
     1501 cgctgtctgt gtgattggga cctggctgat ggcgctctgg aaacgccggg tgacttcgtt
     1561 ccagccgatt cctccaagca agatcacgtg acgggagagg tcgtcactca caacgtcgct
     1621 agcgagtctg tgaaagacat ctagggtcgg attttccccg cgtaggtgcc catagagctc
     1681 gatcagcgca tcgccgtatt gcctcatcct tgtgaagttc gggtcctgta cgtccccgag
     1741 tggaccctgc acgtccgttg gggcgttcgg gcagatcacg atcaccggcc cggcgtcaaa
     1801 ctggaaagtg cgtcgcactt cgcggtcctc cgcatgaaac agctccaaca actgggattc
     1861 cagttctcgg aaccggtcaa gctcgcctgg cgtcagttga tcttctggta tcaggtgggg
     1921 ttccccctct aatgatcgcg gggtgcagaa gaagcgtgcg taaagcggtg attctggctc
     1981 ccgacggcgt cttgggatta gtcgtggatt cccaagagct cagcgttgcc gctgccacgc
     2041 ggccctcagc gctgagcgct ttcgcgagct gactttgggt tagcacgtgg tcaggccaag
     2101 tggattcgcg cagattgcgc agagcgcgcg ccaatctgat cgcctggttg agctttaggc
     2161 tcacgttcgt cctttctctc tgaagagaac ggccaagact agcccactga aagggtttga
     2221 aatagccccg accgcgcggt gcggccaccg cgcgcgttgg tgccggtggt ggtccggctg
     2281 cgcgccgctg gctgggacgt gttctatgac gccagcggac tcgatgactt cgacgagatc
     2341 cgcgagacga tgcgtcgagc gctcgccgac tcggttctgt tcgtcgcgcg gttcagcgac
     2401 gtctatccgc tgcgccgtgc atgcgcgtga gagctgacga ccgcgctgct ggccgacccg
     2461 gcgagcaggt cctggccgat cgttgaacac ttcgttcgaa gactgccggc tactaccgtt
     2521 gaacggtcgt gaggtcaggc accggtcgag ctataccagt gttatgccag atctacccac
     2581 gtctgctgca gcaataggta aggctaggtg agcaataggt gaaatctacc tcagctagct
     2641 cgccctgtgc cgggcgttga gaaaatgccc accaccgcat ccatgaatcg gtcgttctag
     2701 gtcgttctag gcgggctaga cacgccctta gtatccgctg acggcctgga ggccgtacct
     2761 gttgtgaccc ggcagccgat cgcgatcgcc tacgcgaaca tcccccgcat catcactctc
     2821 tgcagcagtc cctggctcgc taggtcatga cggctggcat agacgggcgc ccatgggctg
     2881 gcacactcga gcaacagctc tgcgaccatg agccgataac ctccgtgcgt tccgtcaacc
     2941 agtagaggcc atgcgat
//
LOCUS       DBNGSB010000049        12000 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3405940, whole genome shotgun sequence.
ACCESSION   DBNGSB010000049 DBNGSB010000000
VERSION     DBNGSB010000049.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 12000)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12000)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12000
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3405940"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2074)
                     /locus_tag="AC159B_01400"
     CDS             complement(<1..2074)
                     /locus_tag="AC159B_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018348612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HIR3376443.1"
                     /translation="MSTKVVERLRGRVGESAARVDGVPKVKGQFLYGSDLWAEEMLWG
                     YTLRSPHPHARIRSIDISQALTGPGVHAVLLAGDVPGKKTFGLDFADQPVLARDRVRY
                     EGEAVAIIAAAELELARRAAARIKVDYEVLPAITDMERALRPDAPRLHEFGNVLRHVR
                     IVHGDPKAQADVWVEGYYETGMQDQAMLGPESGLAIPAPDGGIDLYVATQWLHVDQEQ
                     VAPCLGLSKDQVRLHLAGVGGAFGAREDVSMHIHACMLALRTRKPVKMSYTREESFYG
                     HVHRHPARIWMRHGAARNGRLLNVYARLLIDGGAYASSSTAVIGNASTFAAGPYEVPN
                     ALVEGTAVYTNNPPCGAMRGFGAVQACFAYEAQMDKLARILSTDPVELRLKNVLQTGS
                     KLPTGQIVRGSAPVRTIIEKCRAIPLPAESGERHDPIDYPGGSGNVSRGERLRRGVGF
                     AVGYKNIGYSEGFDDSSECRIKLFAGSTGPIAEVHSAAAEVGQGVHTILAQVVRSELG
                     IDNVVLHPSDTAVGSAGSSSASRQTMMSGGAAQLAAVAVRTALLDRVRQWAQKTGRAL
                     RGPLSLGGQDVLDAGAPLARIQQFLEPPIDETRVYHHRKTRAIDKDGQGDPHVTFAFA
                     AQRAVVEVDEELGLVRVLQIATAQDVGKAINPQSVYGQIEGGTAQGLGLALMEEIQLH
                     DGKIRNASF"
     gene            complement(2071..3471)
                     /locus_tag="AC159B_01405"
     CDS             complement(2071..3471)
                     /locus_tag="AC159B_01405"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HIR3376444.1"
                     /translation="MTQTRKRANAPTRQPTNSLLIRDCDVVVTMDDAGTEIAGGSILI
                     ENGAVTWIGTGKPPRPSFQSVDGRGAVAIPGLINAHHHLYQSLTRVRAQDQGLFGWLQ
                     ALYPLWAGIDREWMRSSALVGLAELALSGCSTTTDHHYVFPRGVTGILEEEVDVAREV
                     GLRFHPCRGSMDLGRSQGGLPPDSIVEDAERILTETEDAINHLHDSRPGAMLRISVAP
                     CSPFSVSPRLMRESATLARRHGVRLHTHIAETIDEEAYCRQKFDRRPIELLDDLDWLG
                     RDVWLAHCVHLNGDDIRTLARTGTSVAWCPTSNLRLGSGIAPARELIDAGAAVGLAVD
                     GSASNDSGHMLAEVRQAMLVIRGRAGAAAMTAREALHIATRGSATCLGRDDIGSLEAG
                     KRGDVALFRTGGLAFVGAETDLVAALVFGPPQRVRDLFVEGRPVIRNERLQNVREEDI
                     IKEGSLRARRLLSVEA"
     gene            complement(3468..3932)
                     /locus_tag="AC159B_01410"
     CDS             complement(3468..3932)
                     /locus_tag="AC159B_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015100420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3376445.1"
                     /translation="MIVKTRVNGEHCEAEVWPGASLLFFLREKLGLVGSKNACEQGEC
                     GSCSVWLDGELVCSCLVLAAQAHGREVRTVESLASGERLHPVQEAFLETGAVQCGFCT
                     PGLIVAVADLLEHDPRPRESVIREALAGNLCRCTGYAKIIDAVRIAAQRMQK"
     gene            complement(3947..4825)
                     /locus_tag="AC159B_01415"
     CDS             complement(3947..4825)
                     /locus_tag="AC159B_01415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR3376446.1"
                     /translation="MDVLLPRTLDEALEMKASMPHAVSIAGGTDLMVDLNFDRKRPDV
                     IIEISRVPELKVSRRDNGTFFLGAGVTYSQIVRDLPQFRALIEASRTVGSPQIRNRAT
                     VGGNLGTSSPAGDALPVLSAYDAEIVIVKAGRKTRRLPFAKFMTGPKKNALAPDELIL
                     GAQWTIVHGPGSFSKIGTRNAMVIALASLCLVVDEERAAVRVALGSVGPTILRAGDAE
                     AFADRAMEKAGVWKDPFAPLPPQVVEEFGEHVAAAARPIDDVRGTAAYRRHACAVLAR
                     RALRWALDGRKLTATR"
     gene            complement(4926..5540)
                     /locus_tag="AC159B_01420"
     CDS             complement(4926..5540)
                     /locus_tag="AC159B_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376447.1"
                     /translation="MRTRLKSLLVVASIIAAGCQSVTVQVPAEADLQGVKTVAVVASD
                     LPNDPDPVAVLLRSEASSRIRRLLPTLTLVEPTGTPDAVLNMKVVRHGITPVSFRVGV
                     DPQTGRVSCRAWQVASLMVDGSVSTDQTIRWQGLLEAGRRIDLFCLPAGGVVVPGSQG
                     SIDPRLVHDVVDELGRRLAGYARREFRAIQNPDTTPTPIPAPDP"
     gene            5630..7243
                     /locus_tag="AC159B_01425"
     CDS             5630..7243
                     /locus_tag="AC159B_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3376448.1"
                     /translation="MFGQAAQEALQTVGEPPDMPAGQAPISPSGVDRTARAPRLSVRN
                     VTKTFPGVTANANVSLDVHGGEVHALLGENGAGKTTLMNILYGIYQPDAGEIFIDGRR
                     IDIRSPRDAIGHGLGMVPQHFLLVRRHTVAENVALGLRETRFLFPVRSVEQQIREAGE
                     RYGLAVDPQAYVWQLSVSEQQRVEILKALLRGAEILVLDEPTSVLTPQEAKQLFAVLK
                     RMKVEGHAIIFITHKLDEVIEAADRVTVLRKGAVIDTQPISAVDKRVLARMMVGRDVE
                     FQMPRRSDARSDRDALVVQDLWVRDDRGLAAVRGVSFAVRQGEVLGIAGVAGNGQREL
                     IEALTGLRTSERGRVAVPAGEVTNRSAKTLYEAGVAHVPEERMRTGIVPQMAVSENLV
                     LKRYRYPPFSRGPLIQLPAVDDFARRMIAEYGITTPGPRTPARLLSGGNVQKLILARE
                     LSAQPGLIVAAHPTYGLDVSATEQIHHTLLDRRNEGAGVLLVSEDLEEIFALSDRIAV
                     LFRGEVMGIVAARALERETLGLMMAGQRA"
     gene            7240..8355
                     /locus_tag="AC159B_01430"
     CDS             7240..8355
                     /locus_tag="AC159B_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013456762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3376449.1"
                     /translation="MRQLGPLRFEHDPEPSLTRTVLISALAIAAAFLVAGLIFWAYGV
                     SPLRAYRVILVDTLTDPRALPEIFVPAIPLLLTGIGLLLAFRAQFYNIGAEGQLLAGT
                     IAATWIAVFTSTPDPLMLPLMFLAGLVAGAVWGLLPSLLKLRLGVNEVITTLMMNYIA
                     LYSVSWLVTGPWKGPSVRGFAYTDTFPDAAWLPYFPGTRVHWPTLLLGIALAILVTFL
                     LNRTRFGFEIRVQGHNPDAARYAGINPLRTTLLVMLLSGGLAGLAGVGEVAGIHHKLL
                     DPNQVSLGYGYVAIIAAWLARGNPLAVIVTAIFFGLIYSSGDVMQVSLQMPARVTDVF
                     NGLILFFLIGSERLLQYRVRWVIQRGETPRLGEQWKS"
     gene            8343..9215
                     /locus_tag="AC159B_01435"
     CDS             8343..9215
                     /locus_tag="AC159B_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3376450.1"
                     /translation="MEIVAAITGILIRGLAFGTPLLWGSLGEIYAERAGVVNLGVEGM
                     MILGAFSGFATAHVTGNPVAGLCAAALVGALAALLHAFVAITLRANQYVSGLALSMFG
                     LGLSGLLGRGWEGIPLERPLPEVTILAPVGFALAIALWFVLYHTRWGIILRTTGEAPA
                     AADAMGINVILVRYAAVVFGGLMAGIAGGFLSVAYRPSWTEGMTSGIGWIVVALAIFA
                     SWDPLKAIWTSLLFGVLFHLSFRLQTWIPAEPLQMMPFAFTIIVLTVTARRGGGARQA
                     APEALGLPYVRGER"
     gene            9261..10421
                     /locus_tag="AC159B_01440"
     CDS             9261..10421
                     /locus_tag="AC159B_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018466568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3376451.1"
                     /translation="MRWIKLLAIVAALLVVATTGWAQPSKVKVGFIYVGPIGDYGWTN
                     AHDVARKKLERAFPGQVETMYVESVPEGNAEPFIDKLVRGGANVVFTTSFGFMDGTLA
                     AAKRHPNVIFAHASGFKRNDNMATYMADFYQIYYLNGLMAGALTKTNKIAYVGAFPIP
                     EVKRHMGAFALGVRAANPRATVHVRWLFSWFDPAKAKEATEALLAERADVFAFTEDSP
                     TVIQVAAQKNRTSFSHYSPMQRFAPRHVISGQLVHWDRIYVDFIGKVLKGTYSNQNLE
                     SVDYWWLLAEKAVELGGQFGQPINPAFTGQLKAARVKTREFGTISVYDLVNRRLAQMA
                     RKPVAFDPFTGPIRDRKGVVRVPAGHTMTIGELNSMEWAAPGIVGQWPKEPK"
     gene            complement(10472..10936)
                     /locus_tag="AC159B_01445"
     CDS             complement(10472..10936)
                     /locus_tag="AC159B_01445"
                     /inference="COORDINATES: protein motif:HMM:NF020907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HIR3376452.1"
                     /translation="MRDLPRKLTSDELAILFEGRTNFTRRLAEHENPLAVARRLLTEI
                     PEDELIDALNAHPRIGGRPASTRSAREQGAEEDSAILSGLAKLNQTYEDRFGFRFVVF
                     VNRRRRSEILRVLQQRLNRTREQELLTAIDDLVSIAEDRYRSGALKGNGGRR"
     gene            complement(10912..11211)
                     /gene="uraH"
                     /locus_tag="AC159B_01450"
     CDS             complement(10912..11211)
                     /gene="uraH"
                     /locus_tag="AC159B_01450"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019588781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033971 - hydroxyisourate hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006144 - purine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HIR3376453.1"
                     /translation="MPSISTHVLDTDRGTPARGVRVELYRGDRLLSAQQTNDDGRISD
                     LAQGPLESATYRLVFHVSSPFFSRLEVEFKITDTSRHYHIPLIVAPYLCATYRGS"
     gene            complement(11211..11783)
                     /locus_tag="AC159B_01455"
     CDS             complement(11211..11783)
                     /locus_tag="AC159B_01455"
                     /inference="COORDINATES: protein motif:HMM:NF005592.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HIR3376454.1"
                     /translation="MPHILPYNGQTTYDLKIAGTVRPLPLIQVAPDTWIAYYYSLGDT
                     EVIDRAARELAPKLSNCELFVTTETKGIPLAHAIAANLGLRPYVVCRKEIRPFMLSPL
                     VVRYKPITAKTEQELYLDGRDAMKVRGKRVGIIDDIVSTGETLDAMAELVRQAGGTVV
                     ARAALLMEGDERADVHHLGILPIFKSKSPA"
BASE COUNT         2199 a         3838 c         3692 g         2271 t
ORIGIN      
        1 tgaatgacgc gttacggatc ttgccgtcgt ggagctggat ctcttccatc aacgcgagcc
       61 caagaccctg cgcggtgccg ccttcaattt gtccatacac gctctgagga ttgatcgcct
      121 tgccgacgtc ctgcgctgtg gcgatctgaa gcacacgcac gagcccgagc tcttcgtcta
      181 cttcaaccac ggcgcgctgc gcggcaaacg cgaatgtcac gtgaggatcc ccctgaccat
      241 ccttatcaat cgcgcgcgtc ttccgatgat ggtagacgcg agtttcgtca atcggcggtt
      301 ccaggaattg ctgtatccgc gcgagcggcg cgccggcgtc gagcacgtcc tgcccgccga
      361 gcgacagcgg cccgcggagc gcccgtccgg tcttctgcgc ccactgccgc acccgatcaa
      421 ggagcgcagt ccgaaccgcg acggcagcga gttgggcggc gccacccgac atcatcgtct
      481 gccgcgaagc ggagctcgaa cccgcggagc ctacggccgt atcggaggga tgcaggacaa
      541 cgttgtcgat gccgagctcg gaccgcacca cctgagcaag gatcgtgtgc acgccctggc
      601 cgacttccgc cgcggcgctg tggacttccg cgatggggcc ggtcgatccg gcaaacaact
      661 tgatgcggca ctctgacgag tcatcaaacc cttcgctgta cccgatgttc ttgtagccta
      721 cagcgaatcc aacgccccgt cgcagtcgtt cacctcgact aacgttcccg gagccgcccg
      781 gataatcgat gggatcgtgt cgctctccag actcggcggg cagcgggatg gcccggcact
      841 tctcaatgat cgtacgaacc ggcgcgcttc cacgaacgat ctgtccagtc ggcagctttg
      901 aacccgtctg cagtacattc ttcaggcgga gctcgaccgg gtcggtcgaa agtattcggg
      961 cgagtttgtc catctgggcc tcgtaagcaa aacacgcctg caccgctccg aaaccccgca
     1021 tcgccccgca cggtgggttg ttggtataga ctgcggtgcc ttcgacgagc gcattcggca
     1081 cttcgtacgg cccggccgca aacgtggagg catttccgat caccgcggtc gacgacgacg
     1141 cgtacgcgcc tccgtcgatc agaagtcgcg cgtatacatt cagcaatctc ccgttgcgtg
     1201 cagcgccgtg gcgcatccat atcctggcgg ggtggcggtg cacgtgtccg tagaatgact
     1261 cttctcgtgt gtatgacatc ttcaccggct tgcgcgttcg aagcgccagc atgcaggcat
     1321 ggatgtgcat gctgacatcc tcgcgcgccc cgaacgcgcc gcccaccccc gcaaggtgca
     1381 accggacctg gtctttggac agcccgaggc atggggccac ctgctcctga tcgacgtgga
     1441 gccattgtgt cgcgacatag agatcgatgc cgccatccgg cgctggaatc gccagccccg
     1501 attccggtcc gagcatcgcc tgatcctgca tgccggtttc gtagtagccc tccacccaca
     1561 cgtccgcctg agctttggga tccccgtgga cgattcgcac gtgacggagg acgttgccga
     1621 actcatgcag ccgtggcgca tccggccgaa gtgcccgttc catgtcggtg atcgcgggca
     1681 gaacttcgta gtcaaccttg atcctggcgg cagcacgccg ggcaagctcc agctcagccg
     1741 cggcgatgat ggcgacggct tcgccctcat agcgcacgcg gtcacgcgcg agcaccggct
     1801 ggtctgcgaa gtccagacca aacgtcttct ttcctgggac gtctccggcc agcagcacgg
     1861 cgtgaactcc agggccggtc agcgcctgcg agatatcaat cgaccggatt cgcgcatgcg
     1921 gatgcgggct ccgaagcgtg tagccccaca gcatttcctc ggcccacagg tcgctgccgt
     1981 acagaaattg acctttgacc ttgggaacgc cgtcaacgcg ggccgcactc tcaccgaccc
     2041 tgcctcgcag ccgttcgact actttcgtgc tcacgcttcc acgctcaaca accgcctggc
     2101 ccgaaggctt ccttctttga tgatgtcctc ttcacgaacg ttttggagac gctcgtttcg
     2161 tatgacgggg cgtccctcaa caaaaagatc gcgaacccgc tgcggcggac cgaagaccag
     2221 cgctgcgacc agatcggtct cggctcccac gaatgccaga ccgccggtcc tgaacagggc
     2281 gacgtctccg cgcttcccgg cttcaagtga gccgatatcg tcgcgcccca ggcacgttgc
     2341 gctgccgcgc gtcgcaatgt ggagtgcctc tcgggccgtc atcgccgccg ctccagcacg
     2401 gccccttatt acaagcatcg cctgacgaac ttcggccagc atatgaccgg aatcgttcga
     2461 ggcagagccg tctacggcaa gcccgaccgc tgcgcccgca tcgatcaact ctcgcgccgg
     2521 agcaatcccg gaccccaggc ggaggtttga agtcggacac cacgcaacgg aggtgccggt
     2581 acgcgcgaga gtgcggatgt cgtcgccatt caggtgcacg cagtgcgcca gccacacgtc
     2641 gcggccgagc cagtccagat cgtctagcag ttcgatcggc cgtcggtcga acttctggcg
     2701 gcagtaggcc tcttcatcaa tcgtctcagc aatgtgcgta tggagacgca cgccgtggcg
     2761 gcgagcaagt gtggcggact cacgcatgag gcgtgggcta accgaaaacg gcgagcaggg
     2821 ggcgaccgag atgcgcagca ttgcccccgg ccgagaatcg tgcaggtggt tgatcgcgtc
     2881 ctcggtttca gtcaggatcc gctctgcatc ttcgacgatc gagtccggcg gcaacccgcc
     2941 ctgggatcta ccgaggtcca tcgagccgcg acacgggtga aagcgaagcc cgacctcgcg
     3001 ggctacgtcg acttcctctt cgaggatccc ggtgaccccg cgggggaaca cgtagtgatg
     3061 gtcggtggtt gtcgagcagc ctgacagggc gagctcagcg agccccacga gggcggacga
     3121 tcgcatccac tcacgatcga tccctgccca aagcggatag agagcctgca gccagccgaa
     3181 gaggccctgg tcctgtgcac gcacccgcgt cagcgattga tagagatgat ggtgcgcgtt
     3241 gatcagtccc ggaatggcca ccgccccgcg gccatcgacc gactggaaag atggacgggg
     3301 aggctttccg gtcccgatcc aggtcactgc gccattttct ataaggatag aaccccctgc
     3361 gatctcagtg ccggcgtcat ccatcgtgac cacgacatcg caatcgcgaa ttagaagcga
     3421 gtttgttggt tgccgcgtcg gcgcgtttgc gcgtttgcgc gtttgcgtca tttctgcatt
     3481 ctctgcgccg ctattctcac ggcgtcgatg atctttgcat atcctgtgca gcggcagaga
     3541 ttacccgcca gcgcttctcg aatgacgctc tcccgaggcc taggatcgtg ctcaagcagg
     3601 tcggcgacag cgacgatcag gcccggcgtg caaaaaccac attggaccgc gccggtttcg
     3661 aggaatgcct cctggaccgg atgcaacctc tcgccgctcg ccaggctttc caccgtccgc
     3721 acctcgcgtc cgtgggcctg cgcggcgagc accagacacg agcacacgag ttctccgtcg
     3781 agccacacag agcacgaacc gcactcgccc tgttcgcacg cattctttga accaaccaga
     3841 ccaagtttct cgcgcaagaa gaacagcagg ctggcccccg gccagacctc agcttcacag
     3901 tgttctccat tgacacgtgt cttgacaatc atgtcgcgct accgcgtcat cgcgttgccg
     3961 tcagttttct accatcgagt gcccatcgaa gggcgcggcg tgcgaggacc gcgcatgcgt
     4021 ggcgccggta cgctgccgta ccacgaacgt catcgatcgg gcgcgccgcc gcggccacat
     4081 gctcgccgaa ttcctcgacc acctgtggag gcaggggcgc gaacggatcc ttccacacgc
     4141 ctgctttctc catggctcga tctgcaaacg cttccgcgtc acccgcccgc aaaatcgtcg
     4201 gccccactga gcccagcgcg acacgcactg cagccctttc ctcatccacc accagacaca
     4261 ggctggccag cgcgatgacc atcgcgttgc gagtaccgat ctttgagaag gaccccgggc
     4321 cgtggacgat ggtccactgc gcgccgagga tcagttcatc cggcgcgagc gcgtttttct
     4381 ttggaccggt catgaattta gcaaagggta accttcgggt cttccggccg gccttcacga
     4441 taacgatctc ggcatcatac gcggatagca ccgggagcgc gtcgcctgcg ggagacgagg
     4501 tccccaggtt tccgccgacc gtggcgcggt tgcgaatttg gggagatccg acggtgcgcg
     4561 atgcctcgat cagcgcacgg aactgcggga gatctctgac gatctggcta tacgtcacgc
     4621 ctgccccgag gaagaacgtt ccgttgtccc ggcgtgagac tttcagttca ggaacccggc
     4681 tgatctcgat gatgacgtcg ggacgcttcc tatcgaagtt gaggtccacc atcagatcgg
     4741 tgccgcccgc gatagacacc gcatgcggca tggatgcctt catctccagg gcttcgtcga
     4801 gtgtgcgagg caacaaaacg tccatggcca gattccgcga cgcccgtatc ttccacgaaa
     4861 gaattgcggc acccctgccc cgcgttgcgg gactggagtg ccgcgactac gtcaggaaag
     4921 tgatgttagg gatcgggtgc gggaatgggg gttggagtcg tgtccgggtt ctggatcgcg
     4981 cggaattctc ggcgcgcata gccggcgagc cgtcgaccca attcatcgac gacatcgtgg
     5041 acaagccgag gatcgatgga tccttgagag cccggtacga caacgccccc cgccggtagg
     5101 cagaagagat cgatgcgacg gccggcttcg agcaagccct gccagcgaat cgtttggtct
     5161 gtgctcacgg atccgtcgac catgagcgac gccacctgcc aggccctgca gctcacgcgg
     5221 cctgtctgtg gatcgacgcc cacacggaac gaaacaggcg tgatgccgtg gcggacgacc
     5281 ttcatattga gcaccgcatc gggggtacct gttggctcaa ccagggtcag ggtgggcagg
     5341 agccgccgga ttcgagatga tgcctcactc cgcagcagca ccgccacagg atcaggatca
     5401 ttgggcaggt ctgaggccac gacggccacg gtcttgaccc cctgcagatc agcttccgca
     5461 ggcacctgca ccgtgacaga ctggcacccg gccgcgatga ttgaagccac aacgaggaga
     5521 cttttcaggc gcgtcctcat ggaccacctc cacgttcctc ttgtagcctt cccggccacg
     5581 gcgcacgcta ggcggtggct tgttccttgt tacgcgcgct gggtcagaaa tgttcgggca
     5641 ggccgcgcag gaggcgctgc agacagtggg cgaacccccc gatatgcccg cgggacaggc
     5701 tccgatctcc ccttccggtg tcgacaggac ggcgcgggct ccccggctca gcgtccgtaa
     5761 cgtcacgaaa accttccccg gcgtcaccgc gaacgcaaac gtctctctgg acgtccatgg
     5821 cggcgaggtc cacgcgctcc tgggtgaaaa cggtgccggc aaaaccacct tgatgaacat
     5881 cctctacggc atctaccagc ccgatgccgg agagattttc atcgacggcc gccggatcga
     5941 catccggtcc ccccgcgatg cgattgggca cggcctcggc atggtccccc agcacttcct
     6001 cctcgtccgt cgtcacaccg tggccgaaaa cgttgccctc ggcttgcgag aaacgaggtt
     6061 tctctttcct gtgcgcagcg tcgagcagca gatccgcgag gccggtgaac ggtacggcct
     6121 tgccgttgac ccgcaggcat acgtatggca actgtccgtc agcgaacagc agcgcgtgga
     6181 aattctgaag gcgctgctcc gtggggctga aattctcgtg ctcgacgaac ccaccagcgt
     6241 gctcacccct caggaagcga agcagttgtt cgcggtcttg aagcgcatga aggtcgaagg
     6301 acatgcaatc atcttcatca cgcacaagct tgacgaggtc atagaagcgg cggaccgcgt
     6361 gacggtgctg cgcaaggggg ccgtcatcga cacacagccg atcagcgcgg ttgataagcg
     6421 cgtgctggcg cgtatgatgg tcgggcgcga cgtggagttc caaatgccga gacgcagcga
     6481 cgccagaagc gaccgggacg cgctggtggt ccaggatctg tgggtccgcg atgaccgcgg
     6541 cctggcggcg gtccggggag tctcgtttgc tgttcggcaa ggagaggttc tcggcattgc
     6601 gggtgtggcc ggcaacggcc agcgggagct catcgaagcg ctgactggcc tcaggacctc
     6661 tgagcgaggc cgcgtcgccg tgccggccgg cgaggtgacg aatcgcagtg cgaagaccct
     6721 gtacgaagcc ggcgtcgcgc acgtccccga ggaacgcatg cgtacgggca tcgttccgca
     6781 gatggccgtg agcgagaatc tggtgctgaa gcgctatcgt tatccgcctt tctcacgcgg
     6841 cccgctgatt cagctgcccg cggtcgacga ctttgcccga aggatgatcg ccgagtatgg
     6901 aatcaccacc ccaggaccgc gcacccccgc gcggttgtta tcgggtggca acgttcagaa
     6961 actgatcctg gcacgcgagc tttcggcaca gcctggactc atcgttgccg cccatcccac
     7021 ctacgggctg gatgtcagcg ccacggaaca gatccaccac accctgctcg accggcgcaa
     7081 cgaaggcgcg ggcgtgttgc ttgtctcaga agacctggaa gaaatctttg cgctgtcgga
     7141 ccgcatcgcc gtactgtttc gcggcgaggt gatgggcatc gtcgccgcgc gggccctgga
     7201 gcgagagacg ctcggcctga tgatggccgg acagcgagca tgagacagct tggtccgctt
     7261 cgattcgagc acgacccgga accatcgctg acccggacgg ttctcatctc tgcactggcg
     7321 atcgcggccg cattcctggt ggccgggctt atcttctggg cctacggagt gagcccgctg
     7381 cgggcgtacc gggtcatcct cgtggatacg cttaccgatc cccgtgcact gcctgagatc
     7441 tttgtgccgg ccatcccgct cttgctgacc gggatcggtc tgctgctcgc cttccgcgcg
     7501 cagttctaca atatcggcgc cgaaggacaa ctccttgccg gcaccatcgc cgccacgtgg
     7561 atcgcggtct tcacgtccac ccccgacccg ttgatgctcc cgttgatgtt cctcgccgga
     7621 cttgtcgccg gggcggtgtg ggggttgctt ccgtcattgc tgaagctgcg cctcggcgtc
     7681 aacgaagtca tcaccacatt gatgatgaac tatattgcgc tgtactcggt gagttggctc
     7741 gtcaccggtc catggaaagg ccccagcgtc cgcgggttcg cctacacgga cacgtttccg
     7801 gatgcggcat ggctcccgta ctttccgggc acacgcgttc actggcccac gttgttgttg
     7861 gggattgctc tcgccatcct cgtgacgttc ctgctcaacc gcacacggtt cggattcgag
     7921 atccgcgtgc aaggccacaa ccccgacgcg gcccgatacg cgggcatcaa tcccctgcgc
     7981 acgaccctgc tcgtcatgtt gctctccggt ggccttgctg gactcgcggg cgtcggcgaa
     8041 gtcgccggca tccatcacaa actcctggat cccaaccagg tgtctctcgg ctatggctac
     8101 gtcgcgatta ttgccgcctg gctggcccga gggaatccgc tggcggtcat cgtcaccgcc
     8161 atattctttg gactcatcta cagcagcggc gatgtcatgc aggtgtcctt gcaaatgccg
     8221 gcccgtgtca ccgacgtctt caatggactg atcctcttct tcctcatcgg gagcgagcgt
     8281 ttgctgcaat atcgagtgcg gtgggtcatc cagagaggag aaacgccgcg attgggtgag
     8341 caatggaaat cgtagccgcg atcacgggca tactgattcg cggtctggca tttggcacgc
     8401 cgctgctgtg gggctcactt ggcgaaatct acgccgagcg ggccggcgtc gtgaacctgg
     8461 gagtcgaggg catgatgatc ctcggcgcat tctcggggtt tgcgacggcg cacgtcacgg
     8521 gcaaccctgt agccggactg tgcgccgcag ctctcgtcgg ggcccttgcc gctctgcttc
     8581 acgccttcgt ggccatcacg ctgcgcgcca accaatacgt ctcgggactg gcgctctcga
     8641 tgttcgggtt gggactctcg gggttactcg ggcgtggatg ggaaggaatt cccctcgagc
     8701 gcccactccc ggaggtcacg atcctggcgc cagtgggttt tgcgctggcc atcgcgctgt
     8761 ggtttgtgct gtaccacacg cgatggggaa tcatccttcg aacaaccggc gaggctccgg
     8821 cagcggccga cgctatgggg atcaacgtga ttctggtgcg ctacgccgcc gtcgtgttcg
     8881 gcggactgat ggccgggatc gccggaggct ttctctccgt cgcataccgg ccatcatgga
     8941 ccgagggtat gacctcggga attggctgga tcgtcgtggc gctggccatt tttgcgagct
     9001 gggatccgct gaaggcgatc tggacctcgc tcctctttgg cgtgctcttc cacctatcgt
     9061 tccggctgca gacatggatc ccggcggaac cgctgcagat gatgccgttc gcattcacga
     9121 tcatcgtcct gaccgtcacg gcgcgacggg gcggcggggc tcgccaggcc gcacccgagg
     9181 ccctcgggct tccatatgtc agaggggagc gttgaccggc aggtcgaaac attcagcatc
     9241 acgcacgggg gaggcgagga atgcgttgga ttaaactttt agccattgtt gcggccttgt
     9301 tggtggtcgc cacaaccgga tgggcccagc cgtccaaggt caaagttggg tttatctacg
     9361 tcggccccat cggcgattac ggttggacca acgcgcacga tgtggccagg aagaaactcg
     9421 agcgggcgtt cccagggcag gtagagacga tgtacgtcga gtcggtccct gagggaaacg
     9481 cagagccgtt catcgacaag ttggtgcggg gtggcgcgaa cgtcgtgttc acgacgagtt
     9541 tcggtttcat ggatggaacc ctggcagccg ccaagcgcca tccaaacgtg atcttcgccc
     9601 atgccagcgg cttcaagcgc aatgacaaca tggccacgta catggccgac ttctatcaga
     9661 tctattacct caacggattg atggcaggcg cactgaccaa gaccaacaag atcgcgtacg
     9721 ttggtgcgtt ccccatcccc gaggtcaagc ggcacatggg tgcgttcgcg ctaggcgtgc
     9781 gggccgccaa cccgagggcg accgttcatg tgcgctggct gttcagctgg tttgatcctg
     9841 ccaaggccaa ggaagcaacg gaagcgttgc tggcagaacg cgcggacgtg ttcgcgttca
     9901 cggaagattc gccaaccgtc atccaggtcg ccgcgcagaa gaaccggacg agtttcagtc
     9961 actactcccc gatgcagcgc ttcgcccccc gccatgtgat ctcggggcag ctcgtgcact
    10021 gggatcgcat ctacgtcgac ttcatcggca aggtgctgaa ggggacgtac tccaaccaga
    10081 atctcgagag cgtcgactac tggtggctac tggctgaaaa agccgtcgag ctcggcgggc
    10141 agttcggaca gccgattaac cctgccttca caggccagct gaaagcggca cgggtcaaga
    10201 cgcgggaatt cggaacgatc tcggtctacg atctcgtcaa ccgccgcctc gcgcagatgg
    10261 caaggaagcc ggtggccttc gatcccttta ccggccctat tcgcgaccgg aagggcgtgg
    10321 ttcgggttcc tgcgggacac acgatgacga tcggcgagct caacagcatg gagtgggcgg
    10381 cacccggcat cgtcgggcag tggccgaagg agcccaagta aagacgcaag cgcgcaaacc
    10441 gcgattctac aaataacaga acggcgggcg ctcagcggcg cccgccgttg cctttgagtg
    10501 cgccgctccg gtagcgatct tcggcaatac tgaccagatc gtcgatcgcc gtcaataatt
    10561 cctgttcacg tgttcggttc aggcgttgtt gcaacaccct caggatctcc gagcggcggc
    10621 ggcggttgac gaacacgacg aaacggaagc cgaacctgtc ctcgtaggtc tggttgagtt
    10681 ttgcgaggcc ggagagaatg gcggaatctt cctcggcgcc ctgctcgcgg gctgaccgtg
    10741 tcgatgcggg gcgccctccg atccgtgggt gcgcgttcag cgcgtcaatc agttcatctt
    10801 ccggaatttc agtcagcagc cgcctggcga cagcgagagg gttctcatgc tcagccaggc
    10861 gtctcgtgaa attggttcgg ccttcaaaga gaattgcgag ctcgtctgag gtcagcttcc
    10921 gcggtaggtc gcgcacaggt acggagcgac gatcagcggg atatggtagt gtcgcgacgt
    10981 gtcggtgatc ttgaattcca cctcgagccg cgagaagaac ggcgacgaaa cgtgaaacac
    11041 gaggcggtag gtggcggatt caagtggacc ctgcgccaga tccgagatgc gcccgtcgtc
    11101 attcgtctgc tgggcggaca gcagtcgatc gccacgatac agctcgacac gcacgccccg
    11161 cgccggggta ccccggtcgg tatccagcac gtgcgtggaa attgaaggca ttaggcgggg
    11221 ctcttggatt taaagatcgg aaggatgccg agatggtgca cgtcggcccg ctcgtcgcct
    11281 tccatcagca gcgctgctct ggccacgacc gtcccaccag cctggcgcac gagttccgcc
    11341 attgcatcga gcgtctcgcc ggtgctgacg atatcgtcga tgatcccgac ccgcttcccc
    11401 cgcaccttca tcgcatcacg gccgtccaag tagagctcct gctcggtctt ggccgtgatc
    11461 ggcttatagc gcaccacgag cggggagagc atgaatggtc ggatctcctt gcgacacacg
    11521 acatatgggc gtaacccaag gttggcggca atcgcatggg ccagggggat gcctttcgtt
    11581 tcggtggtga cgaacaactc gcagttcgac aacttaggag caagctcgcg cgcggcccgg
    11641 tcgatgacct cggtatcgcc taggctgtag tagtaggcga tccaggtatc gggcgcgacc
    11701 tgaatcagcg gcagcgggcg aacggtgccg gcaatcttca gatcataagt cgtttgaccg
    11761 ttgtacggca gaatgtgtgg catatgaaac cccacataag ataatagcgc aggaacaatc
    11821 ttcatttcca cggaagccca ctcccaccca aatggaactg gggcagttca ttcaggtcgc
    11881 ccgcggcgcg cagccggccg atctcctcct caccaacgcg cagatcgtcg atctctacaa
    11941 tctcgatgtc gtccgaggac aggttgcgat cgccgacggc cggatagccg gcaccgcgcc
//
LOCUS       DBNGSB010000050         3370 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_187576, whole genome shotgun sequence.
ACCESSION   DBNGSB010000050 DBNGSB010000000
VERSION     DBNGSB010000050.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_187576"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..229)
                     /locus_tag="AC159B_01460"
     CDS             complement(32..229)
                     /locus_tag="AC159B_01460"
                     /inference="COORDINATES: protein motif:HMM:NF018657.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376455.1"
                     /translation="MNHQHRFHTLVAHIRGPVEPVMLGGHEVTAAIPVAVGEGGNMTV
                     YFEVLGVRRRAQDRCDRRSRS"
     gene            complement(150..659)
                     /locus_tag="AC159B_01465"
     CDS             complement(150..659)
                     /locus_tag="AC159B_01465"
                     /inference="COORDINATES: protein motif:HMM:NF014998.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
                     /protein_id="HIR3376456.1"
                     /translation="MDTGKVPQQFAVILILDRPGDFSLSQLRQMFLERTVALPRLRQR
                     LINVPPGCGRPVWIDDPDFQINRHVREVPCRPPGNEQALLDTALSVIMTPLPRWAPLW
                     SAFLITELEDDAAAVVVVLHHVLADGLGGVNVLAAWGLPVLHEPPAPIPHTRGAHPRP
                     RRAGHARRA"
     gene            complement(1093..1950)
                     /locus_tag="AC159B_01470"
     CDS             complement(1093..1950)
                     /locus_tag="AC159B_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3376457.1"
                     /translation="MDNQDQRAEKKYQAKAPRTQTFGEVAFEASGETTLRWLGMAGFL
                     INSRGTTLMVDPLLGGFDMPIMIEMPVATTDVARLDAILVTHSDNDHYSVPTCRDLAP
                     VTRAYHSTKYVASLMNNEGFPAYGHDIGDVFDVGALQVEVTPADHAWQNESPGASDRI
                     FQPEDACGFWVETPDGTIWAPGDSRLIPEHHLQMPAPDALFFDFSDSEWHFGLAGAVK
                     MANAYPNTPLLLYHWGSVDAPDFAPFNGDPESLYDLVKNPERIHVLAPGEPFTLNRWM
                     ETISDPVDG"
     gene            complement(2015..2302)
                     /locus_tag="AC159B_01475"
     CDS             complement(2015..2302)
                     /locus_tag="AC159B_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376458.1"
                     /translation="MARVRERGSHEPERLRHSLRSGIDKPAGGEERHWLHGGPERNVR
                     FWVPVMKCALCIDEAGQEVAMEILEITHACTIPAAPRHPRAREAPGRTTVL"
     gene            2376..2759
                     /locus_tag="AC159B_01480"
     CDS             2376..2759
                     /locus_tag="AC159B_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YybH family protein"
                     /protein_id="HIR3376459.1"
                     /translation="MTKQDLNDVIAGFREALRDYVKGDPEPAMSFFSDWDDVTTANPL
                     EPPRRGAAEVGEAARRAAANFQEGGPLHFEEVSSSFEEVSRFATSELAYVLQIERHEG
                     RVAGHDDTFVTVLRATLVFRCEGGV"
     gene            complement(2904..>3370)
                     /locus_tag="AC159B_01485"
     CDS             complement(2904..>3370)
                     /locus_tag="AC159B_01485"
                     /inference="COORDINATES: protein motif:HMM:NF014026.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HIR3376460.1"
                     /translation="QAVYIENIGSAAAARLGRVVDDPADADLAVLRLNAPYEPRPGGF
                     EAFFHAGSLEFPAAERERIVAICEQVPTIIVLFLDRPAIVPEIAVAAAALLVEFGARD
                     DAVVDVLLGEAQAMGRLPFDLPSSTKAVTDSRSDVPYDTANPLFRFGDGIVD"
BASE COUNT          669 a         1038 c         1029 g          634 t
ORIGIN      
        1 caacttgcgg gtcaagtcgt caaggtctgg gtcatgatcg ggatcgacga tcacagcgat
       61 cctgagcacg ccggcgtaca cccagcacct cgaagtagac ggtcatgttc ccgccttcgc
      121 cgactgcgac cggaatggca gcagtaacct catgcccgcc gagcatgacc ggctcgacgg
      181 ggccgcggat gtgcgccacg agtgtgtgga atcggtgctg gtggttcatg tagtaccggt
      241 agaccccaag ccgctagcac atttacaccg cccaggccgt ctgccaggac gtggtgcagc
      301 acgaccacca ccgctgccgc atcatcttcc agctcagtga tcaagaacgc cgaccacagc
      361 ggagcccatc tcggcagcgg agtcatgatc acagacaggg cggtgtcgag cagcgcctgt
      421 tcgtttcctg ggggtcggca gggtacctcc cggacgtggc ggttgatctg gaagtcagga
      481 tcatcaatcc acaccggacg cccgcagcct ggcggcacat tgatcaaacg ctgccgtaac
      541 cgtggcaggg caacggtgcg ctccaagaac atttgtcgga gctgagacag gctgaaatca
      601 ccgggtcgat ccaagatcaa gatgaccgcg aactgctgcg gaaccttgcc agtatccatt
      661 gcgaggaacg ccaaatcggc cgacctggca cgctcgatat ttggccgacc cgttgtcact
      721 tcagtgcacg cgattgatcg gggatcggtg gagccgaacg gcagtcaacc gccaccactc
      781 ctctcggcct tcggcgctaa ccatccccgg gttctgcagc agcgcgtcgc ggtgcaggcc
      841 cgggatgacg tgatagcgga tcccccgtaa cgcttaccag ggccagcacc cgccacagcg
      901 cccgcgtagt aaccacgcac tttcgcgacg gcgatccagg ccttaccggg tggcagcgga
      961 aactgttgcg acacgatgag ttccatcctt tccacgacga tcccatcgtg gcccgcgcgc
     1021 ggtgtcccag atcatgatca ttggtcgtca gtcgttggga cctttgtcct acgcgacgag
     1081 ccaggtctgc atctagccgt ctaccggatc gctgatggtt tccatccaac ggttcagtgt
     1141 gaagggttcg cccggtgcca ggacatgaat tcgttctgga ttcttcacca gatcgtataa
     1201 ggattctggg tcgccgttga acggtgcaaa gtcgggagcg tccactgatc cccaatgata
     1261 aagcaggagc ggtgtatttg gataggcatt cgccatcttc acggcaccag ccaatccgaa
     1321 atgccattca ctatcagaaa aatcaaagaa tagcgcgtcg ggtgctggca tttgcagatg
     1381 atgttccggt atcaagcgtg aatcacccgg cgcccagatt gtgccatcag gcgtctcaac
     1441 ccagaagccg catgcatcct ctggctggaa aatgcgatca cttgctccag gagactcatt
     1501 ctgccaggcg tgatcagccg gtgttacttc aacctgcagt gctccgacat cgaaaacatc
     1561 cccaatatcg tgaccataag ctgggaaacc ttcattgttc atgagagaag ccacgtactt
     1621 tgtagagtga tatgcgcgag taacgggagc cagatcacga caggttggga cactgtagtg
     1681 atcgttatcg ctatgagtaa ccaatattgc gtccaaacgg gcaacatccg tggttgcaac
     1741 tggcatctcg atcatgatcg gcatgtcaaa ccctcctaaa agggggtcga ccattagagt
     1801 tgtgccgcgg ctattgatca agaatccggc catgcccagc catcgaaggg ttgtctcgcc
     1861 cgaggcttca aacgctactt caccaaaggt ctgtgttctc ggagccttcg cctgatactt
     1921 cttttccgcc ctttgatcct ggttgtccac gtgcccgacc ttacgaccgt cgtcgcttgt
     1981 gaggaagggc ctcgttttgt gaggaagggc ctcgttacag caccgtggtg cgccccggcg
     2041 cctcacgcgc gcgaggatgg cgaggggctg ccggtattgt gcaggcgtgg gtgatctcga
     2101 ggatttccat cgcgacttcc tgccccgctt catcgatgca caacgcgcat ttcatgacgg
     2161 ggacccagaa ccgaacattt cgctctggtc caccgtggag ccagtgacgc tcttcgccgc
     2221 ccgcgggctt gtcgatgccg gatcggagcg agtgacgaag acgttccggc tcgtggcttc
     2281 cacgttctcg aacgcgcgcc acgccgaccg caggccgccc gaccgacccg ccgactgagc
     2341 ggaaccccgt ctggacggag tcatgaggtg atcggatgac gaagcaggat ctgaacgacg
     2401 tcatcgcagg tttccgcgaa gcgctgcggg actacgtcaa aggtgacccg gaacccgcca
     2461 tgagcttctt ctccgactgg gacgacgtca cgaccgccaa cccgttggaa cctccgcgtc
     2521 gaggagccgc ggaggtcggg gaggcggccc ggagggcggc ggcgaacttc caagaaggcg
     2581 gtccactgca cttcgaggag gtgtccagca gcttcgagga agtttcgagg ttcgccacgt
     2641 cggagcttgc gtatgtcctg cagatcgagc ggcacgaggg cagggtcgcc ggccacgacg
     2701 atacgttcgt gaccgtgctg cgcgccacgc tggtcttccg gtgtgagggc ggcgtgtaga
     2761 agatcgcaca ccgccacgca gacccaatta cgtctacgcg accggtcacc accctcctcg
     2821 actcctagag tgcgtatgcg agctcgggcg tgaaggggct ggagtatcag cgacgctcgg
     2881 gctcagcatc gacgagtaat ccttcagtcg acgatgccgt cgccgaatcg aaacagcggg
     2941 ttggcggtgt cgtacggaac gtcgctgcgg ctatctgtca cggccttcgt ggatgacgga
     3001 aggtcaaacg ggagccgccc catcgcttgg gcctcgccga gcaaaacgtc cacgaccgcg
     3061 tcgtcccggg cgccgaactc gaccagcagg gcggccgcgg cgacggcgat ttccggcacg
     3121 atcgccgggc ggtccaggaa gagcacgatg atcgtcggga cctgctcgca gattgcgacg
     3181 attcgctcgc gctccgccgc cgggaactcc agcgagccgg catggaagaa cgcctcgaac
     3241 ccgcctggac ggggttcgta gggggcgttc agccgcagca ccgccaggtc ggcgtcggcg
     3301 gggtcgtcga ccacccggcc cagccgggct gcggcggccg agccgatgtt ctcgatatag
     3361 accgcttggt
//
LOCUS       DBNGSB010000051         6307 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_1107803, whole genome shotgun sequence.
ACCESSION   DBNGSB010000051 DBNGSB010000000
VERSION     DBNGSB010000051.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 6307)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6307)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6307
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_1107803"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="AC159B_01490"
     CDS             <1..590
                     /locus_tag="AC159B_01490"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HIR3376461.1"
                     /translation="RTGVSVFFERVAFVGRQVPGDLQWAAVGPGTADALHAHGIEHVW
                     IPTRYLSDALGDGLPAQRGESVLRIRADAASPVPAARIRSRGIIVHEIVAYRTVEAPP
                     DSVPLLREAFAGGIDGVVFTSASTVRGFAKLIEAAEFQDKVLRLTVIAIGPVTADAIR
                     ALGWPVHLVADEYSVDGIAATLRRRQANASGIVRS"
     gene            565..1560
                     /gene="hemB"
                     /locus_tag="AC159B_01495"
     CDS             565..1560
                     /gene="hemB"
                     /locus_tag="AC159B_01495"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HIR3376462.1"
                     /translation="MLQVSSEVNLVLRPRRMRRTSAVRALVRETRLDPSRFIYPLFIR
                     EGRGIREPLPAMPGQWRVSPDRLAPEVDETRALGIGAILLFGIPTHKDAAGSGAYADD
                     GVIQEAVRAIRTAHPDLVIVTDVCLCEYTSHGHCGIIREESVDNDATLEVLAKTAVSQ
                     ASAGADIVAPSAMMDGQVTALRRALDASEFTDVAIMGYSAKYASAFYGPFRDAAGSTP
                     QFGDRRGYQMDPSNAREAEREIELDLAEGADIVMVKPALPYLDVIHRARSRVTVPLAA
                     YNVSGEYAMLKAASERGWCDERSAVLEVLTAIARAGADLIITYHAKEASQWLTSR"
     gene            1542..2231
                     /locus_tag="AC159B_01500"
     CDS             1542..2231
                     /locus_tag="AC159B_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HIR3376463.1"
                     /translation="MVDEQVRDQTFVQVLALRLDPAWRRLADEERERATAEFARVVES
                     TRREVLMESYSLVGLRADVDLVFMRFASSLVQLEETASQVLGAGMGRWMTVARSFLGL
                     TRPSRYVRKPGAQEQAIISGERGAYLVVYPFTKTHDWYRLSREARQGMMSEHIRVGHD
                     FPQVRQLLAYSTGLDDHEFIVAYETDDLPAFQDLVIALRETEARRFTLNDQPILTGIH
                     RPLQDALRLLR"
     gene            2232..3272
                     /gene="hemE"
                     /locus_tag="AC159B_01505"
     CDS             2232..3272
                     /gene="hemE"
                     /locus_tag="AC159B_01505"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012811364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HIR3376464.1"
                     /translation="MNDRFLRACRREPVDRTPVWFMRQAGRSQPQYRALRERHSLLDI
                     VRNPELGAEVTLRPVHELEVDAAVLFADITLPFLSMGVGFELGDGGPQIHHPIRTDDD
                     VAGVQAFAVDQTIAAVLETIRIVRARSPVPLIGFAGAPFTMASYLVEGGPSKDYLHTK
                     MMMYGQPETWRALMDALTDMTIRYLLIQVEAGAQAVQLFDSWVGCLSPVDYTRYVAPY
                     TRRVLDALRPAGVPAIHFGTGTAGLLALMAGVGGDVIGVDWRIDLDRAWEAIGHDRAI
                     QGNLEPAVLLAPSEVIRERTIDVLRRAAGRPGHIFNLGHGVLPDTPREHLVQVVQTVR
                     AFVAEPEMERTR"
     gene            3269..4201
                     /gene="hemH"
                     /locus_tag="AC159B_01510"
     CDS             3269..4201
                     /gene="hemH"
                     /locus_tag="AC159B_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015254313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - protoporphyrin ferrochelatase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HIR3376465.1"
                     /translation="MTPPFGVLLMAYGSPASLDDVEAYYTHIRGGHPPSQNAVEELRE
                     RYRRIGGRSPLPEITQRQAAGLEAALRVQGIRPRVYAGMKHAPPFISETVAQMAEDGI
                     GHAAGVALAPHYSRMSIGGYIAAATAAASERNVELRCVEHYHDHPGLVHALADRIEIA
                     RGRFPTPRDVPVVFTAHSLPERILEWGDPYPDQLMRTCQLVASAASIERWRFAFQSAS
                     HTGERWLGPDVREALRELREAGAEEVIVSPIGFVADHLEVLYDIDIEARGTAEKLGMR
                     LERAPSLNYDHDFLSVLADVAKAALASFQARARA"
     gene            4204..4365
                     /locus_tag="AC159B_01515"
     CDS             4204..4365
                     /locus_tag="AC159B_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376466.1"
                     /translation="MHPARRATRPALSKARGYRVIVPALLAVLALITVVVLVLAGGVL
                     FGLIPYPGR"
     gene            4375..5115
                     /locus_tag="AC159B_01520"
     CDS             4375..5115
                     /locus_tag="AC159B_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376467.1"
                     /translation="MTQYLPMASAVIIVVFAALVFRRYLQRGGAHLLLWGIGLSMFGV
                     GSLTEAYSTVAWHPSVFRLWYLTGAILNAAWLGQGTVFLLSRRRRTARALLALLIVGS
                     MIATWLIFTTPLNAARFNSQETLSAQYREILPLGAPVRRLTPIFNIYGLLTLVGGALY
                     SAWLLWRKEIVPNRVLGNLLIAVGALALGFASVLVRLGLGDFLYVAEMIAAIFMFGGF
                     LLATSRAGPSTTATAVPSTCVQSLHLAG"
     gene            5112..5729
                     /locus_tag="AC159B_01525"
     CDS             5112..5729
                     /locus_tag="AC159B_01525"
                     /inference="COORDINATES: protein motif:HMM:NF025719.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR3376468.1"
                     /translation="MRLGAASRFLGVDPSTLRAWTDAGRVPVFRTPGGHRRYDERDLR
                     AFLDRNRQKRESVTDIVGPRGARLVDMHLRDRVRAQDWYQAFDPKAISAMRSTCQRIM
                     DGLAGYLAGGSRLSPSLREGERAGHALGAHVARLSMTPSMATQAFLFFRKMVTDAAAR
                     RLPLSPDLKVQSLQRLDVYLNQVLLEMMKAYERQSDREHGSAESR"
     gene            5741..>6307
                     /locus_tag="AC159B_01530"
     CDS             5741..>6307
                     /locus_tag="AC159B_01530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3376469.1"
                     /translation="MDRTLILIPVFNEEATLAGVLLQVRKAAPDADVLVVDDGSTDRS
                     PEILAARPDIRVIRHEDNRGYGQSLITGFTDGTTRGYEIILTIDCDEQHEPRRIQAFR
                     DRVGQSDIVSGSRYLDPSVPGDPPPHDRMQLNREFTALIRAVTGYEITDAWCGFKAYR
                     VEGLRRLHLTEPSYGMPLQVWVQAAFHRL"
BASE COUNT         1088 a         1917 c         2074 g         1228 t
ORIGIN      
        1 gccggaccgg cgtcagcgtc ttcttcgagc gcgtcgcttt tgtggggagg caggtgccag
       61 gtgatctcca atgggcggcc gtcgggccgg gcacggcgga cgctctacac gctcacggca
      121 tcgagcatgt ctggattccg acgcggtacc tcagtgacgc actgggagac gggttgcccg
      181 cgcagcgtgg agagagcgtg ctgcggatcc gagcggacgc agcgtctccg gtgccggccg
      241 ctcggatccg gtcgcgaggc ataatcgtcc acgaaatcgt tgcctaccgc accgtggagg
      301 cgccgccgga ctcagtgccg ctcttgcgtg aggcattcgc tggcgggatc gacggcgtcg
      361 tctttacgag cgcctcgacc gtgcgcggat tcgctaagct gattgaggct gcggagttcc
      421 aggataaggt tctgcgcctt accgtcatcg cgatcggtcc ggtgacggcg gacgcgatcc
      481 gcgcattggg atggccggtg catctggtcg cggacgaata cagtgtcgat ggcatagcag
      541 caacgctccg aaggaggcag gcgaatgctt caggtatcgt ccgaagttaa cctcgtgctg
      601 aggccccggc ggatgcggcg cacctccgcc gtgcgcgcac tggtgcgcga aacgcgtctc
      661 gacccgtcac ggttcatcta cccgttgttt atccgcgagg ggcgcggaat ccgtgagccg
      721 cttccggcaa tgccgggaca gtggcgggtc tcccccgacc gtctcgcgcc ggaggtcgac
      781 gaaaccagag cgctcgggat cggggcgatt ctgttgtttg gcatcccgac gcacaaggat
      841 gccgcgggca gcggcgcata tgccgacgac ggtgtgatcc aggaggcagt gcgcgcgatc
      901 cgcacggcgc atccggatct cgtgatcgta actgacgtct gtctgtgcga atacacgagc
      961 cacgggcact gcgggatcat ccgcgaggag tcggtggaca acgatgcgac attggaggtg
     1021 ctggcaaaga cggctgtttc acaggcctcg gccggtgccg atatcgtcgc gccgagtgcc
     1081 atgatggacg gtcaggtcac cgcccttcgt cgcgcgctcg atgcgtcaga attcactgat
     1141 gtcgcgatca tggggtattc ggccaaatat gcgtcggcgt tctacggccc gttccgagac
     1201 gccgccggct cgactccgca gtttggggat cgtcgcgggt accagatgga tccctcgaat
     1261 gcgcgtgagg ccgagcgtga gatcgagctc gaccttgccg aaggcgccga tattgtcatg
     1321 gtcaagccgg cgttgccata tctcgatgtc atccaccggg cgcggtcacg cgtgacggtt
     1381 cccctggcag cctacaacgt cagcggcgaa tacgcgatgc tgaaggccgc ctcggagcgc
     1441 ggatggtgtg atgaacgtag cgcggtgttg gaggtcctca ccgcgatcgc tcgggcgggc
     1501 gccgacctta tcatcaccta ccacgcgaag gaggcatcgc agtggttgac gagcaggtaa
     1561 gagatcaaac gttcgtgcag gttctggcac ttcggctcga tccggcatgg cgccggctcg
     1621 ccgacgagga gcgagagcgg gcgacggctg agttcgctcg tgtcgtggaa tccacgcgtc
     1681 gcgaggtgtt gatggagagt tactcgctcg tcggcctacg cgccgatgta gatctcgtct
     1741 tcatgcgctt tgcctcctcg ttggtgcaac tcgaagagac cgcttcgcaa gtgctgggag
     1801 ccggcatggg tcggtggatg accgtggccc gttctttcct cgggctgacc cgcccatccc
     1861 gatacgtgcg gaagccggga gcgcaggagc aggccatcat ctccggggaa cgcggtgcgt
     1921 atctcgttgt gtatccattc accaaaacgc acgactggta ccgactcagc cgtgaagcaa
     1981 ggcaggggat gatgagcgag cacatccgcg tcgggcacga ttttcctcag gtgcgccaac
     2041 tgctcgcgta ctccacgggg cttgatgacc atgagtttat cgtcgcctat gagaccgacg
     2101 acctcccggc gttccaggat ctcgtcatcg cgctgcggga gaccgaggcg cgacgcttca
     2161 cgctgaacga ccagccgatt ctcaccggaa ttcatcgacc gctccaggat gcgctgaggc
     2221 tgcttcggta gatgaacgat cggttcttac gcgcgtgcag gcgagagccg gtggaccgga
     2281 cgccggtctg gttcatgcgg caggccggac ggtcgcagcc gcagtaccgc gcgctgcgag
     2341 agcggcactc cttgctcgac attgtccgga atcccgaact cggcgccgag gtgacgctgc
     2401 ggccggtcca cgaacttgag gtggatgccg cggtgttgtt cgcagacatc acgctgccgt
     2461 tcctcagcat gggcgtaggc ttcgagctgg gggacggcgg accgcagatt caccacccga
     2521 tccgtaccga cgacgatgtc gctggcgtgc aggcctttgc cgtcgatcag acaatcgccg
     2581 cagtgctgga aaccatccgg atcgttcggg cgcgctcgcc ggtcccgctc atcggtttcg
     2641 cgggcgcgcc gtttactatg gcgagctatc tggtagaagg cgggccgtcc aaagactacc
     2701 tgcacacaaa gatgatgatg tacgggcagc cggagacgtg gcgggcgctc atggatgctc
     2761 tgactgatat gacgatccgc tatcttctga ttcaggtcga ggcaggcgct caggcggtgc
     2821 aactcttcga tagctgggtc ggctgcctca gccccgtgga ctacacaagg tacgttgcgc
     2881 catacacgcg ccgcgttctc gacgccctcc ggccggccgg cgttccggca attcactttg
     2941 gcaccggaac cgctggcctg cttgcgctga tggcgggggt tggaggtgac gtcatcggtg
     3001 tcgactggcg catcgatctc gatcgcgcgt gggaggcgat cggccatgac cgcgcgatcc
     3061 aaggaaacct cgagcccgct gtgctgctcg cgccgtctga ggtcatccgt gagaggacaa
     3121 tcgacgtgct tcgccgggca gcaggtcgtc ccgggcacat tttcaatctc gggcatggcg
     3181 tgctgcccga cacgccgcgc gagcatctgg tgcaagtcgt ccagaccgtg cgcgccttcg
     3241 tggcagaacc ggaaatggaa cggacccgat gacgcctcct tttggcgtgc tcctcatggc
     3301 ttacggaagt ccggcatcac tcgacgatgt cgaggcctat tacacgcata tccgtggcgg
     3361 acacccccct tcacagaatg cagtagagga gttacgggaa cgttatcgcc gcattggtgg
     3421 acgctcccca ctgccggaaa ttacgcagcg gcaagcggcg gggcttgagg ccgcgctgcg
     3481 ggtgcagggg attcggccac gggtgtacgc tggaatgaag cacgctccgc ccttcatctc
     3541 ggagacggtc gcgcagatgg cggaggatgg tatcggccat gctgctgggg tggcgcttgc
     3601 tccccactat tccagaatga gcatcggcgg gtacatcgcg gccgcaacag cggcggcgtc
     3661 tgagcgaaac gtcgagttaa gatgcgtgga gcactatcac gatcatcccg gcctcgtcca
     3721 cgcgcttgcc gatcgcatcg agattgccag gggccggttc cccacgccgc gcgatgtacc
     3781 ggtcgtcttc accgcgcaca gcctgcctga gcgcattctt gaatggggcg atccatatcc
     3841 ggatcaactc atgaggacct gccagctcgt ggcgtcggcc gcgtccatcg aacgctggcg
     3901 gtttgcgttc cagagcgcga gccataccgg cgaaaggtgg ctggggcccg acgtccgtga
     3961 agcgctccgt gagctccgtg aagcgggagc ggaggaggtc attgtctcgc ccatcggctt
     4021 tgtggccgac cacctggaag tgttgtacga catcgatatc gaggcgcgcg ggaccgccga
     4081 gaagctcggc atgcggctcg agcgcgcccc gtcgctgaac tacgaccacg actttctgtc
     4141 ggtcctcgcc gatgtcgcga aggcggcgct cgcgtcgttt caggcgcggg cgcgtgcata
     4201 gtcatgcacc cggctcggcg cgcgaccaga cctgcgctgt ccaaggcaag aggctaccgg
     4261 gtcattgtgc ctgcactgct ggcggtgctg gcgctgatca ctgttgtggt gctcgtgctc
     4321 gcgggcggag tgcttttcgg actcattccg tatccaggga ggtaggcgcg ccacgtgaca
     4381 cagtatttgc ccatggcctc ggccgtaatc atcgtcgtgt tcgcagcgct cgtgttcaga
     4441 aggtacctgc agcgaggcgg ggcacatctt ttgctgtggg gcatcgggtt gagtatgttc
     4501 ggcgtggggt ctctgaccga agcgtattcc acggtggcct ggcatccttc cgtgttcagg
     4561 ttgtggtatc taaccggcgc aatcctgaac gcggcctggc ttgggcaagg gacggtattc
     4621 cttctgagtc gacgcaggcg tacggcgcgc gcattactcg cgctgctgat cgtcggcagc
     4681 atgatagcaa cgtggctcat cttcacgaca ccactcaatg ccgcccgctt caactcccag
     4741 gagacactca gcgcgcagta ccgcgagatc ttgccactcg gtgccccggt ccgaaggctg
     4801 acgccgatct tcaatatcta cgggctgctg acgctcgtcg gtggagcgct gtactccgcc
     4861 tggttgctgt ggcgaaagga gatcgtcccc aaccgcgtct taggaaatct cctcatcgcc
     4921 gtcggtgcac tcgcgctggg cttcgccagc gtcctggtca ggttgggcct gggtgacttt
     4981 ctgtatgtgg cggaaatgat cgccgcgatc ttcatgtttg gtggatttct gcttgccacg
     5041 agccgcgcgg ggccatccac gacagcaacg gcggtgccgt ccacatgcgt gcagtcactc
     5101 catctagctg gatgagactc ggcgcggcca gccgcttcct tggcgtcgat ccatcgacgc
     5161 tgcgtgcgtg gacagacgcc ggacgtgttc ccgtctttcg caccccggga ggccaccggc
     5221 gttatgatga gcgagacctg cgcgcattct tagaccgcaa ccggcagaag cgtgagtcgg
     5281 tgactgacat agtcgggccg cgcggcgcta gactggtcga tatgcatctc cgagaccggg
     5341 tacgggcgca ggattggtat caagcgtttg atccaaaggc gatctccgcg atgcggagca
     5401 cctgccaacg catcatggat ggcttagccg gctatctggc gggaggatcg cggttgtctc
     5461 cgtcgcttcg agagggggaa cgcgccgggc acgcgctcgg agcgcacgtt gccaggctca
     5521 gcatgacccc gtccatggcc acccaggcct ttctgttctt tagaaagatg gtgaccgatg
     5581 ccgcggcgcg ccgccttccg ctctcacccg acctcaaggt gcaatcactc caacggcttg
     5641 atgtctacct gaatcaagtg cttcttgaga tgatgaaggc ctatgaaagg caaagtgacc
     5701 gggagcacgg gagcgcggaa tcgcggtagc gcccggctct atggatcgga ctctcatcct
     5761 catcccggta ttcaatgaag aggcgaccct tgcgggcgtc ctcttgcaag tcaggaaggc
     5821 tgcgccggac gccgacgtat tggtagtgga tgatggctcc acggatcgct ctcctgagat
     5881 tcttgccgca aggccggata ttcgagtgat ccgccatgag gacaatcgcg ggtacgggca
     5941 gtcgctgatc actggattta ccgacgggac cacacgggga tacgagatta tcctcacaat
     6001 cgattgcgat gagcagcatg aaccgcggcg tatccaggcg ttccgcgatc gcgtaggcca
     6061 gtccgacatc gtctcaggca gccgatatct cgatccgtcc gttcccggcg atccgccgcc
     6121 gcacgaccgg atgcagctga accgtgagtt caccgccctc attcgagcgg tcaccggata
     6181 tgagatcact gatgcgtggt gcggattcaa agcgtaccgc gtcgaaggac tcagaagatt
     6241 gcacttgacc gagccgagtt acggcatgcc gctgcaggtt tgggtgcagg cagcctttca
     6301 ccggctc
//
LOCUS       DBNGSB010000052         5265 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3254770, whole genome shotgun sequence.
ACCESSION   DBNGSB010000052 DBNGSB010000000
VERSION     DBNGSB010000052.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 5265)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5265)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5265
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3254770"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="AC159B_01535"
     CDS             complement(<1..427)
                     /locus_tag="AC159B_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376470.1"
                     /translation="MGKKSIERLMTLPELSEMLGVPIETLYGWRHRGDGRSGIGSAVT
                     SATAARQLRNGWRRKPTVATRWVGKTVAYIERRQLEQRDKSGRTRSVVRYKVRYRDAA
                     GKPHSETKRRLVDAERRKAEIELEVGNGLWRDPRRGEIRL"
     gene            complement(1836..3035)
                     /locus_tag="AC159B_01540"
     CDS             complement(1836..3035)
                     /locus_tag="AC159B_01540"
                     /inference="COORDINATES: protein motif:HMM:NF022062.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIPR family protein"
                     /protein_id="HIR3376471.1"
                     /translation="MTGSGGDTGQDAIAILVNGELITDPDEIDTLASSGATLDVNYIF
                     VQTETSTSFSTSKVGQIAYGVKDFFSDSPTLERNEIVAAAAEISQKVLSNARLFRNGN
                     PTCSVYYVTAGRWTGDNDLSARIDSARSDIDELNLFSKVIFAPLDAREVQRRYQRLRT
                     GAEREFTFQNRIALPEISHVSESHLGYVPVTDLLSILTDDDGLLMSTAFYENVRDFQG
                     EANPVNAEIASTLAGDRRSQFPLMNNGVTIIAKSVRQTGTKFVIRDFQVVNGCQTCNV
                     LWSRREQLDESILVPLRLISTDDEDVIVDIIRATNRQTEVKEEQFFATSDYLKQLEMY
                     FESTPLDRRLYLERRSKQHANTPVERTRVVPFNNLVRSFASIILVSPIAPHETISKFS
                     NASRMTS"
     gene            complement(4041..4715)
                     /locus_tag="AC159B_01545"
     CDS             complement(4041..4715)
                     /locus_tag="AC159B_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376472.1"
                     /translation="MDQPSVAPRRAWASERWAPLAGVAAVVLWVIGILVDPGIPDLFV
                     ATGQEWLTFVSGNEGGILTSRLLLLIGDFLFILFLGTLRTRLFAAEAEPRHWTAIAFG
                     GGIATAAMLIATSTPVLAAAAAADGLEPSAAQALGVVEYAFFVGAEIAGAVLLVATGM
                     LAIRTAVLPTWLGWASLVVALLMLTVIGPIGFIAIVIGLPLWVLAVSILLWRRGRAGG
                     SSSPGQ"
     gene            complement(4837..5064)
                     /locus_tag="AC159B_01550"
     CDS             complement(4837..5064)
                     /locus_tag="AC159B_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376473.1"
                     /translation="MGPRKVDRATWPSAGAFFVGCASAAVGVDGVGAEDLAGGEVDDG
                     DAGLVGDGEDAAVGVGGAEAEVVHPARLVGS"
BASE COUNT         1034 a         1597 c         1588 g         1046 t
ORIGIN      
        1 cgagtcgaat ctcgccgcga cggggatcgc gccatagtcc gttgccgact tcgagctcga
       61 tctccgcttt tctgcgttcc gcgtcgacca ggcgccgctt tgtctcactg tgcggtttgc
      121 cggcggcatc gcggtaacgc accttgtagc ggacgaccga tctcgtgcgc ccggacttgt
      181 ctcgctgctc aagctggcgg cgttcgatgt acgccacggt cttaccaacc catctggtcg
      241 cgacggtcgg cttgcgtctc cagccattcc tcaactgccg cgcggcggta gcggacgtga
      301 cggccgatcc gatacccgac cgtccatcgc ctcggtgccg ccagccatac agcgtttcga
      361 tcggaacgcc aagcatctcg gacaactctg gcagagtcat cagccgctca atcgatttct
      421 tacccatgat catcttcctt tctcatccgg atgcgtcggt ggtccgccgg tcgaagctag
      481 cgatggaacc tttccttctc aggcactgga cagctcctat ctagccacgc cggacggaca
      541 ggtggctggt tcacgccgag ctgtggataa ccggtgccgc tgcaaacccc ctgcagactt
      601 cccaaggtga gcaaccgttg ctggcggtca ctctctgttg aaagaatttc ttgcagaggg
      661 gacttcgtga gcctgtcggc gctcaaacga tctccgtccc accgcggtca cccaggctgc
      721 agtcgccatc gctcgtcggc aaatccgccg tccgcatcgg cgctgcgatg cgttttgccg
      781 agccgcgatc cgcgaccgcg gacgaaaggg cgtgggcatg gcgtctgcac ggatccatgc
      841 acggccaaga acgtcctctg ccagtcacca aggttcgcgt taatgtggcg cggttgccat
      901 cagcgctgcc gtccagagat cggccacgag tttgatatgc gcttcgcgag tgaggcggcg
      961 cggttggatc tctggtcccg cccgcgacga gtccactgac cacttccatg ccatcgacaa
     1021 tggggtgccg tgatttgccc gctttgagcg cgggaagtgg acgcagatgt gcctgcatgt
     1081 gcggcgtcga gcgcactgat tggtaagcag attggtaatc agcgttcgtc gtcaagctga
     1141 ccttctcgtt gcgagcgttg cgcttggagc aagagccttg tccgggacta ttcacgaccg
     1201 tgcatctgat gcatgctgat catgttcgtc gcgattcggg ggcacaccgg gggcacacgt
     1261 tccggcgcgc cgtgactcct actgtgtcag ggcaggctgc gtcgatggga ccacattgtc
     1321 ggcagcggaa actgtatgcg tgcgagttac cccgcattcg aggaatagcg cggcgaatgg
     1381 ctgtctgtct ggctgtcagg ggcccggcac ttgactagtc gcgccggcct gagcaatcca
     1441 ggacgctcct agagggcgca caccgtccta cgcccctacg cgggcgttgg agcggtagtg
     1501 cgatagaact tgctccgtga acggttgcgt ccgaatccga tcacgtgata gatcttcgtc
     1561 tgcaagggca gtgatgtccg cagccgcagt acgaaagagt acctcagctt tcggtgggtc
     1621 ccagtagacg tcgatcaaat ccctggccca ggaatcggct tcgcgactgt tcaggaacgg
     1681 cggtatctct ggcttgacaa tcaagcggct cgccaagagg agatggtact tggcggaagt
     1741 gaagcgccta tcaagcaccc cgttccgaaa caagaattcg atgcggtata gcgatgaagc
     1801 ggcagcgagg tatagcgagg ccttgtgact tgggttcaag atgtcattcg ggatgcgttc
     1861 gagaacttgc ttatagtttc gtgtggcgcg atggggctca ctaagatgat cgaagcaaaa
     1921 gagcggacta gattattgaa gggaactacc cgcgttcttt cgacgggcgt gtttgcatgc
     1981 tgtttcgagc gtcgttcgag gtaaaggcga cggtcgagtg gggtgctctc gaagtacatc
     2041 tcaagttgtt tcaagtagtc gctcgttgca aagaattgct cttcctttac ctcggtctga
     2101 cgatttgttg ccctaattat atccacgatc acatcttcgt catccgtact gatcagtcgg
     2161 agtggcacaa gtatggactc gtcaagttgc tcacgtcttg accacagaac gttgcacgtc
     2221 tgacatccat tgacaacctg gaaatcccgt atgacaaact tcgttcctgt ttgccgaacg
     2281 gactttgcaa tgatggtcac accgttgttc attagaggga actggctgcg ccggtctccc
     2341 gccaaagtag aggcgatctc ggcgttaaca gggttcgcct caccctgaaa gtcgcggacg
     2401 ttctcataga aagctgtcga catcaagagg ccgtcgtcgt cagtaaggat cgagagaaga
     2461 tcggtcacag ggacgtaccc gaggtgcgac tctgacacgt gactaatctc aggaagagcg
     2521 atccggttct ggaaggtaaa ttctctctcg gctcccgtcc taagtctttg gtagcgccgc
     2581 tgaacctctc ttgcatcaag cggtgcaaat atgaccttgg agaacaggtt cagttcatca
     2641 atgtcgctgc gagccgaatc gatgcgcgcg ctcaggtcat tatcgccggt ccaccttcct
     2701 gctgtaacgt agtacacgga gcaggttgga tttccattgc gaaagagtcg ggcgttcgaa
     2761 agcaccttct gtgaaatctc agctgccgcc gcgactatct cattccgttc cagcgtgggc
     2821 gagtcggaga agaagtcctt gaccccgtag gcgatttgac ccaccttgga tgtggagaag
     2881 gacgtcgagg tctcagtttg aacgaagata tagttgacat ccaacgttgc tcccgacgag
     2941 gccagtgtgt caatctcatc ggggtcggtg atcaattcgc cgttcaccag gatggcaatc
     3001 gcatcctgtc cggtatcgcc gcccgaaccc gtcatgatgt tttcgtagtc gatggcggcg
     3061 tcgatttgag gactaataac gacgtacgcc gagaatcgct cgaaatcctg agtagggtca
     3121 gttgacggca tggcggtctc gttgagaaac tcggtaacta gcgccttagt gatttggtca
     3181 agtgccacgc cacagaacct ttcagttgaa agcggcccgc cctcgcctag cgttgacgtc
     3241 caatcaggac gccagccttc accctaattc atgctcggac ccgtgtatgc cttcggccga
     3301 cggaacagat ctgaggctct tcccggtccg ccgtgtcggc ggtagaagtg atgcggagtg
     3361 gcgggagttc atcccgttgc ctgggatttc gcccagcagg tcccgcacgg ctgggattga
     3421 ttggatcggt gcctatgcag cgcccaagca acggtgtgtg cgcgctctcc agcaactggt
     3481 taaggactga cggacccagc gatgaacgtc gacgaagtcc tagctccgcc gaggaccgga
     3541 ccgcaggcag cgcgccgagc gcaagcgagg cgtcttgatc agttagagcg gaattcgtcc
     3601 tgcacacggc tggtgatcgg tggagctaag gggactcgaa ccccctgacc ttctcgttgc
     3661 gaaggctcct cctgcgcccg agtcgatagt ccgcaagcgt ccagaccgtt gcaaacgatg
     3721 catggaggtc gcggtgtaac aggtcgggag cacaccggga gcacacgcgt gcggcggcgt
     3781 ctgaccgctg ccttatcagg cgagcccctg cagtggtgaa gtggactcgc gccgcgagcg
     3841 cccgtgacct gtgccgcgct gggccttggt ctggcagtta tcgaacgggg caattatgga
     3901 cacagcaaac caagcgacgg aactcagacc tcacggtctc cggcgtgccg aatcggctcg
     3961 cgatcgttca gaacccgcag tcgcgtgacg tccacctgcg accgccgggt aacggctagg
     4021 cctagctcgg cggccccgga ctactggcct ggcgaactag aaccaccggc tcggcccctc
     4081 cgccagagca ggatgctcac tgcgaggacc cagagcggga ggccgatgac gattgcgata
     4141 aacccgatcg gaccgatgac ggtcagcatc aggagcgcga cgaccagact cgcccagccg
     4201 agccaggtgg gcaggaccgc cgtgcgaatc gcgagcattc cggtcgcgac aaggaggact
     4261 gccccggcga tctccgcgcc gacgaagaag gcgtactcca ccacgccaag cgcctgagcg
     4321 gctgagggct ccaaaccatc ggcggccgcc gccgccgcga gaaccggcgt cgaggtcgca
     4381 atcagcattg ccgccgtcgc gatcccgccc ccgaacgcga tcgccgtcca atgccggggc
     4441 tccgcctcgg ccgcgaaaag ccgtgtccgc aacgttccga gaaacagaat gaagaggaaa
     4501 tcaccgatga gtaacagcag cctggacgtg aggatcccgc cctcgttgcc actaacgaag
     4561 gtcagccact cctgtcccgt cgccacgaac aagtcgggaa tgcccggatc gacgagaatg
     4621 ccgatcaccc agagaacgac tgcggcgacg ccggcgagcg gcgcccatcg ctccgatgcc
     4681 caggcccgcc gcggggccac cgacggctgg tccatccagc ctcctctcaa ccggcaccaa
     4741 ctcatcgtag gcaggcggga cgagagtggg ctgtacagga gaaaagtaca agcctctacc
     4801 ggctgcgcta cgaccgcacc gacgaccacg gcaaaatcag cttccgacga gccgggccgg
     4861 atgcaccacc tcggcctcgg cgccgccaac gcccaccgcc gcgtcctcgc catcgccgac
     4921 cagaccagcg tcaccgtcat cgacctcacc accggcgagg tcctcagcac ccacaccatc
     4981 gaccccaacc gcagcactgg cgcaaccaac aaaaaacgcc ccggccgacg gccaggtggc
     5041 tctgtcaacc ttgcgtggcc ccatgtgggc agaggtagct ggacccaggt ggtgctggct
     5101 ccggagatgt tgagcgttgg ttcacgatga ggtctagtct tgttttctgg cgtgccggga
     5161 agcctggtcg gcgttcttgt tgtcgaccgt tgaggggttc tggtgaaggt tctggtcatg
     5221 actttgggca cgcgcggaga cgtccagccg tttgtggcgt tggct
//
LOCUS       DBNGSB010000053         3985 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2488348, whole genome shotgun sequence.
ACCESSION   DBNGSB010000053 DBNGSB010000000
VERSION     DBNGSB010000053.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3985)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2488348"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="AC159B_01555"
     CDS             <1..287
                     /locus_tag="AC159B_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376474.1"
                     /translation="RGRIYSLPDFDGQLSVALDPTKWEGVDGREGSFRGDVRGQIRSA
                     LYARLREMEDLREHPWLIGWVNDPRARVMLSPGDAGPALQLLAARVETSK"
     gene            403..1128
                     /locus_tag="AC159B_01560"
     CDS             403..1128
                     /locus_tag="AC159B_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376475.1"
                     /translation="MRLNPVYRNDVRFRLTGPKAINVVSIYLTLLGVLAMLTLPPDVG
                     QLDELRQEGLLRAMLIVQMVLVAYFTSATACGEIVIDGEKSAWDLAASPFPSPVIARG
                     KVATSVAFASMLIFIGLPIVTTVGALRGESLWPVALAPLVGLPFAAGAGSLGTLYSVW
                     FDSDFTRGFVHWLTLAALVIASAALPAPWNLISPARSLQLIDRGIGPAMVLVVLGYLL
                     IAAIAAMLVRSRLDVIRRDALAS"
     gene            1185..2732
                     /locus_tag="AC159B_01565"
     CDS             1185..2732
                     /locus_tag="AC159B_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376476.1"
                     /translation="MTIEPGRELLRLVRQAAVRLTVSRAGQAAARAGALAMAAFVLLN
                     VAGVVIPVSGVPMAHAIAAAGLAALLAGVLVVLVRPVPRVDTARALDATLRLEERVST
                     AMEVLASALPRSLLAKRVVTDAERRLSAVGVAAVTPLQVPRTFWWTAALTALLFVLTS
                     WLHGITIPGTPAHRTAEVIRKEGQRLEQFARTLQSRTRAEHMPATRHLTPQIRDLGSR
                     LQRERLARAEALARIEELSQQLEQTRREIDERLQASRPAPSRERDPSDALRRQSTQRQ
                     IRQLRELLSRLQQDSSSASRDALDQLNQMAAGGEGTQPARVRDQLQRAREQLERGNMV
                     GARDAVSDAMLDLQGLESLMADSEAVRNAQQQVERSQAAIAGGAPVEGQPEGQETEQT
                     SESPVAPGEQRPSASQGEDSEPPQGPNEGSTPGTGQGNEKIGARTPRLDTTKTPQRVR
                     GAQSEGPVASSEIVGTGRSSSSRVQAADVSPAVIARADQAMTRSRTPARYRDIVRRYF
                     QRLAKLR"
     gene            2783..3766
                     /locus_tag="AC159B_01570"
     CDS             2783..3766
                     /locus_tag="AC159B_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3376477.1"
                     /translation="MTPEEFAKVFDRLRREIQKVIVGHQDLLDHVLVALLSGGHVLIE
                     GVPGLGKTLLVKTLAQALDLRYGRIQFTPDLMPADIIGTNVVMQDATGRRYFQFERGP
                     VFTQILLADEINRATPKTQSALLEAMQEHAVTAAGAAYTLEEPFFVMATQNPIEMEGT
                     YPLPEAQLDRFLFKLKVEFPPVPDLMEIIDRTTRPDLPQATVVAKGDEVLQMMRLARE
                     VEVATHVKAFVARVIEATHPRPGASTMARRYVRYGSSPRGAQAMILSAKVRALMAGRA
                     NVSFGDLKALAAPSLRHRLVMNFEGEAEGIDSDAIVANVLEETPELEGVKR"
     gene            3770..>3985
                     /locus_tag="AC159B_01575"
     CDS             3770..>3985
                     /locus_tag="AC159B_01575"
                     /inference="COORDINATES: protein motif:HMM:NF013998.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR3376478.1"
                     /translation="MATLLEPSFLRKLDTMTLRSRRLFRGMLRGERRSRSLGRGVEFA
                     DYRSYQSGDDYRYIDWNIYSRLDRLFIK"
BASE COUNT          697 a         1215 c         1335 g          738 t
ORIGIN      
        1 tgcgtggtcg catctacagc ctgcctgact ttgacgggca actctcggtt gcgctcgatc
       61 ctaccaagtg ggaaggcgtc gatggccgag agggttcctt ccgcggcgac gtccgtggcc
      121 agatccgatc cgcgctgtat gcccgcctgc gtgaaatgga ggatctgcgg gagcatccgt
      181 ggctgattgg ttgggtgaac gatccgcgcg cgcgtgtgat gctctcgccg ggggacgccg
      241 gtcctgctct gcaattgctc gccgcccgag tggaaaccag caaatgaagc tggagagctg
      301 gaaagttgga gagctggaga gtaaaaagat cgttctgata gaaaaatctg ttgctttcca
      361 gctttccaac tcttcagctt ttcagctggg agtgaggact cagtgcggtt gaaccccgtg
      421 taccgaaacg acgtgcgatt ccggctgacc ggtccgaagg cgatcaatgt cgtcagcatc
      481 tatctgaccc tgctgggtgt gctggcgatg ctgacgctcc cgccggacgt tggacagctg
      541 gacgagctac ggcaggaagg gttgctgcgg gcgatgctga tcgtgcagat ggtgttggtg
      601 gcgtacttta cctctgccac tgcctgtggc gagatcgtga tcgacgggga gaagtctgcc
      661 tgggacctgg cggcgtcccc gtttccatcg ccggtcatcg cacgcgggaa ggtagcgact
      721 tctgttgcgt tcgcgtcgat gctcattttc atcggactgc ccattgtgac cacggtcggg
      781 gccctccgcg gcgaatccct ctggcctgtc gcgctcgcgc cgttggtggg actgcccttt
      841 gcggccggag caggctcgct gggtacgctc tacagcgtct ggttcgactc ggactttacg
      901 cgcggattcg tgcactggct gacgctggct gcgctggtca tcgcgtcggc ggcgctgccg
      961 gcaccatgga acctcatcag ccccgcgcgg tctctgcagt tgattgaccg cgggattgga
     1021 ccggccatgg tgctggtcgt tcttggttac ctgttgatcg ccgccattgc cgccatgctg
     1081 gtgcgctctc ggctcgatgt gatcaggcgc gacgcgctgg cgagctgatc taaagtaggc
     1141 agtcgccagt agccagtaga cagtctactg gtattattgt tggtatgacg atcgaaccgg
     1201 ggcgcgagct ccttcgactg gtccgccagg ctgcggtgcg gttaacggtc tcgcgtgccg
     1261 gccaggcggc ggcgcgcgcg ggcgcgctcg ccatggcggc gtttgtgctc ctcaacgttg
     1321 ccggcgtcgt cattcctgtt tcaggggtgc cgatggcgca cgcgatcgcc gcggcaggcc
     1381 tggcggcgct cctcgccggg gttctcgtag tgcttgtccg tcccgttccg cgtgttgaca
     1441 ccgcccgtgc gctcgatgcc accctgcgtc tcgaagagcg ggtgagcacc gcaatggagg
     1501 tcctggcgag cgctcttcca cggtccttgc ttgcgaagcg cgtcgtgacg gatgccgaac
     1561 gacgtctgtc agcggttggt gtcgcggctg tgacgccgct gcaagtcccg cggaccttct
     1621 ggtggacggc cgcgctcacg gctttgttgt ttgtgctgac atcatggctg cacgggatta
     1681 cgatccctgg tacacctgcg caccgcaccg ccgaagtgat ccgtaaagaa ggtcagaggc
     1741 tcgagcagtt tgcgcgtacg ctccaatccc gtacccgcgc agagcacatg ccggcgacac
     1801 gccacctgac gccccaaatt cgcgacctcg gctccaggct ccagcgggag cgtctcgcgc
     1861 gcgccgaggc gctcgcgagg atcgaggaac tctcgcagca gctcgaacag acgcgcagag
     1921 agattgacga gcgtctgcag gcgtcgcgcc cggcgccctc gcgtgagaga gacccgtccg
     1981 atgcattgcg gaggcaatcg acgcagcgcc agatccgtca gctccgggaa ttgctgtctc
     2041 ggctgcagca ggattcctcg tccgcgtccc gcgatgcgct ggaccaactc aaccagatgg
     2101 ccgcgggcgg cgaaggaacg cagccggcgc gcgtcaggga tcagctgcag cgcgcgcgtg
     2161 agcagttgga gcgcgggaac atggttggag cgcgtgacgc agtttcggat gcgatgctcg
     2221 atctccaggg actcgagtcg ctcatggcag actctgaggc tgtgaggaac gcgcagcagc
     2281 aggtcgagcg cagccaggcg gccatcgccg gcggtgctcc cgtcgagggg cagcccgaag
     2341 ggcaggagac cgaacagacc tcggagtcgc ctgtcgctcc gggtgagcaa cggccgagtg
     2401 ccagccaggg agaggatagt gaaccgcccc agggtcccaa cgagggctcg acgcccggga
     2461 ccggtcaagg taatgagaag atcggggctc ggacgccgag actcgacacg accaaaacgc
     2521 cgcagcgcgt tcgcggcgca cagtcggagg ggccggttgc cagctctgag attgtgggca
     2581 cggggcgttc cagcagcagc agggtacagg ccgccgacgt gtctcccgcg gtcatcgcgc
     2641 gggctgatca ggccatgacg cgatcgagaa ctccggcgcg atatcgagac atcgtacggc
     2701 gatacttcca gagactcgcg aagctgagat aagtgacgca catgcgcaag cgcgcaaacg
     2761 cataagcgcg acgggagagt tcgtgacacc ggaagagttc gcaaaagtat tcgaccggct
     2821 ccgcagggag attcagaagg tgatcgtcgg tcatcaggac ctgctcgacc acgtgttggt
     2881 cgcgctgcta tccggcggcc acgtcctcat cgagggggtg cccgggcttg ggaagacgct
     2941 cctcgtgaag acgttggccc aggcgctcga cctccggtat gggcggattc aattcacgcc
     3001 cgacctcatg ccggcggaca tcatcgggac gaacgtggtg atgcaggacg cgaccggccg
     3061 gcggtacttc cagtttgagc gcgggcccgt gttcacgcag attctcctcg cagacgaaat
     3121 caaccgcgcc actccgaaaa cgcaatcggc cttgttggaa gcgatgcagg agcacgccgt
     3181 caccgccgcc ggcgcggcct ataccctcga ggagccgttc tttgtcatgg ccacccagaa
     3241 tcccattgag atggaaggca cctatccgct tcccgaggcg cagctggatc gcttcctgtt
     3301 caaactgaaa gtcgagttcc cgccggtgcc agacctgatg gagatcatcg accgcaccac
     3361 gagacccgat ctcccccagg ccacggtcgt cgccaaaggc gacgaggtgc tgcagatgat
     3421 gcggctcgcc cgcgaggtcg aggtcgcgac gcatgtcaag gcgtttgtcg ctcgcgtcat
     3481 cgaggccacg cacccccggc cgggtgcctc gacgatggcg aggcgatatg tgcgctacgg
     3541 cagcagtccc cgcggggcgc aggcgatgat cttgtccgca aaggtgcggg cgttgatggc
     3601 cggacgcgcc aacgtgagct ttggtgacct gaaggcgctc gctgctcctt cgctgcgcca
     3661 tcgactggtg atgaactttg agggagaagc cgagggaatc gattcagacg ctatcgtggc
     3721 aaacgtcctc gaggaaaccc cggaactaga aggagtaaag aggtaatcgg tggccacact
     3781 cctcgagccg agtttcctgc gcaagctcga cacgatgacg ctccgcagcc gccggctgtt
     3841 ccgcggcatg ctgcgaggag agcgacggag ccggtccctt ggacgcgggg tggagttcgc
     3901 cgactaccgc agttatcaga gcggcgatga ttaccgctat atcgattgga acatttattc
     3961 ccgcctggac cgtctcttca tcaag
//
LOCUS       DBNGSB010000054         2579 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3409109, whole genome shotgun sequence.
ACCESSION   DBNGSB010000054 DBNGSB010000000
VERSION     DBNGSB010000054.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3409109"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="AC159B_01580"
     CDS             complement(<1..748)
                     /locus_tag="AC159B_01580"
                     /inference="COORDINATES: protein motif:HMM:NF046269.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGH1-like glycoside hydrolase domain-containing
                     protein"
                     /protein_id="HIR3376479.1"
                     /translation="MGDYTKPSGRLYPHQWNWDSAFNALGWAHVDWERAASEVESLLR
                     AQWTNGMLPHIRYNPAVIDYAPGPEWWPNTPVKRAGELTSGISQPPVLSSMVYAVGLI
                     QPDEARRKFWWHSIYDALSESLLYFSRHRTTGNSPLIVLVHPWESGLDNSPRWDAAVA
                     KGVRPSRPYRRVDTTKVAAAERPTSRDYDLYMFLVELIATHRYEIRDYLGRTPFAVYD
                     AMFNAVWFRAAKDLNRIALAIDRPPVVSKSS"
     gene            complement(807..1883)
                     /locus_tag="AC159B_01585"
     CDS             complement(807..1883)
                     /locus_tag="AC159B_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013157175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3376480.1"
                     /translation="MADIRLEQVRKVFGKVLAVADVSLNIREGEFMVLLGPSGCGKTT
                     LLRCLAGLERVDGGEVLIGGEDVTDRPPGKRGIAMVFQSYAVFPHMTVLDNIAFGLRM
                     QRVPDDEVQRRASEGAELLQIQQLLGRYPAQLSGGQRQRVAVARAIVMRPRVLLMDEP
                     LSNLDALLRLQMRGELKRLHQEVRSTTVYVTHDQVEALSLGDRIAVMKDGKIVQLDIP
                     DRVYDSPATRFVGSFIGSPPMNFLEGTIRSSDGARAVETAGVRVEYHGVPAGRRDVVL
                     IGIRPEHIAVERSPAADGIPAAVVVVEPIGPQKLLTVQVGSSTLKVTTPVDFAVDQGQ
                     RVWLRFDPSRIRLMDPQTGDAIAS"
     gene            complement(1890..>2579)
                     /locus_tag="AC159B_01590"
     CDS             complement(1890..>2579)
                     /locus_tag="AC159B_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018466416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3376481.1"
                     /translation="PRSLVPSPFSVETMRFFVQAQDVLQSLLNSVVVALLTIVLAVAI
                     GAPAGYALSRFRFGGKEVFRLGILGTKMFPATILAVPLAVSFINWNLYDTLLGVALVH
                     TALALPFAVVITTSVFVGVPFELEEAAMTLGCSRGQAFRKVAMPIALPGLAAAAIFTF
                     VLSWNEVFAATILTLRNRTLPALVMNAVGLAGAPIDYRFAGGFFMIIPALIIIFVIRR
                     YLLTLWGVTVR"
BASE COUNT          514 a          834 c          790 g          441 t
ORIGIN      
        1 acgacgactt cgaaactacg ggcggccggt caattgcgag ggcaattcga ttgaggtcct
       61 tcgccgcccg aaaccacacg gcgttgaaca tcgcgtcgta cacagcgaac ggcgtcctgc
      121 ccagataatc gcgtatttcg tagcggtggg ttgcgatcag ttctaccagg aacatgtaga
      181 gatcgtagtc gcgagaggtc gggcgctcgg cggccgccac tttcgtggtg tccacccgcc
      241 ggtacggcct gctcggtctg accccttttg cgacggcggc atcccatcgg ggactattgt
      301 ccagtccgct ttcccacggg tgcacgagga cgatcaaagg tgagttgccc gttgtacgat
      361 ggcgtgagaa gtagagcagg gactcagaaa gcgcatcata gatgctgtgc caccagaatt
      421 tgcggcgtgc ttcatccggc tgtatcaggc cgacggcgta caccatcgag gacaagaccg
      481 gcggttgaga aatgccgctg gtgagttctc cggcccgctt caccggggta ttgggccacc
      541 actccggacc cggagcgtag tctatcaccg cgggattgta gcggatgtgc gggagcatcc
      601 cgttggtcca ctgcgccctc agtaacgatt cgacctcgga cgcggcacgc tcccagtcca
      661 cgtgggccca gccgagcgcg ttgaatgcgc tgtcccagtt ccactggtga ggatacaggc
      721 ggccgctggg tttggtgtaa tcgcccacat cgttcgcgcg gaggatcccc tccgcgccgg
      781 ccaggagttc ctgatctgtt actgggttag gaggcgatgg cgtccccggt ctgaggatcc
      841 atcaaacgga tccgtgaggg atcgaagcgc agccagacgc gttggccttg atcgaccgca
      901 aagtccaccg gtgtggtgac tttcagtgtc gatgagccca cctgcaccgt gaggagtttc
      961 tgcgggccga tgggttcgac gacgaccacc gcggcgggga taccgtccgc cgcaggcgac
     1021 cgctcaacag cgatatgttc gggcctgatg ccgatcaaca cgacgtcacg ccggccggcc
     1081 ggcacgccgt ggtattccac gcgcactccc gcagtttcaa ccgcacgggc cccgtcggaa
     1141 gatcgtatcg tgccttccag aaagttcatt ggcgggctcc cgatgaagct ccccacgaag
     1201 cgcgtggcag gcgagtcata gacacggtcc gggatgtcca attgcacgat cttcccatcc
     1261 ttcataaccg cgatgcggtc gccgaggctg agtgcctcca cctgatcgtg ggtcacatac
     1321 actgtggttg aacgcacctc ctggtgcagc cgcttcagct cgccgcgcat ctgcaggcgc
     1381 agcagcgcgt cgaggttcga gagcggctcg tccatcagga gcactctagg ccgcatcacg
     1441 atcgcgcgcg cgacggccac gcgctgacgc tgaccgccgg atagctgcgc cggatagcgc
     1501 cccagcaact gctgaatctg cagcagctcg gcgccttcgc ttgcgcgccg ctggacctcg
     1561 tcgtccggca cgcgctgcat gcgcagacca aaggcaatgt tgtcaaggac cgtcatgtga
     1621 gggaaaacgg cgtaggactg aaacaccatg gcaatgccgc gtttgcccgg tggacggtcg
     1681 gtgacatcct cgcctccgat gaggacctct cccccatcta ctcgctccaa ccccgccagg
     1741 caccggagca gcgtcgtctt gccgcacccg ctcggcccca gcagcaccat gaactctccc
     1801 tcgcggatat tcagggagac atcggcgacg gcgaggacct tcccgaagac cttgcgcacc
     1861 tgttccagac ggatgtcagc catggtacct caccgcaccg tgacgcccca cagcgtcagc
     1921 aaataccggc ggatcacgaa gatgatgatc agcgcaggga taatcatgaa gaatccaccc
     1981 gcgaaccggt agtctatcgg cgcgcccgcg agcccgaccg cattcatgac gagcgccggc
     2041 agcgtccggt tgcgcagcgt caggattgtc gccgcaaaca cttcattcca tgagagcacg
     2101 aacgtgaaaa tcgccgcggc ggcgaggccg ggaagggcga tcggcatggc cactttccgg
     2161 aatgcctggc cgcgcgagca tcccaacgtc atcgcggctt cctccagttc aaatggcacg
     2221 ccgacaaaga cactcgtcgt gatcacgacg gcaaacggaa gcgccagcgc cgtgtgtacc
     2281 agggcgacgc ccaacagcgt gtcgtagagg ttccagttga taaacgagac cgccagcggc
     2341 acggcaagga tcgtcgcggg aaacatcttc gtcccaagga tgccgagccg gaacacttct
     2401 ttcccaccga agcggaacct cgagagcgcg taccccgcag gcgccccgat cgcaaccgcc
     2461 agcacgatgg tcagtaaggc gacgacgacg ctgttgagca acgactgcag cacatcttga
     2521 gcctgcacga agaagcgcat cgtctcaacc gagaacggcg agggcacgag cgatcgcgg
//
LOCUS       DBNGSB010000055         3149 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_496139, whole genome shotgun sequence.
ACCESSION   DBNGSB010000055 DBNGSB010000000
VERSION     DBNGSB010000055.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_496139"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..1452)
                     /locus_tag="AC159B_01595"
     CDS             complement(64..1452)
                     /locus_tag="AC159B_01595"
                     /inference="COORDINATES: protein motif:HMM:NF024948.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HIR3376482.1"
                     /translation="MTLLWSRGPMVAGRGFLRSALPFAGVFIEFCWLYPWLLLLSGAF
                     YGGSSRPLLGPWPSFALLLGGFITVRWAVSRSWPISTARVVVAAAGLIAGLLSVKRAY
                     YPHVAAWDLSWIGSLLRAAHDVFPAFAPPVVGALIATMLWWRGIVLGERDFNHFEIDR
                     QYRRGVGWSMFFVLLYAIYGHTRGIALILHAPVYLLVFFSLGLTSLAIARLISIWQET
                     QADEEQALATNRHWILLLIGVVGVILSLAVAISGVVSVEFRPIMLQALRPLAPIVEYL
                     FYIVFAVALVIARAILFVFSQLPFRRDRAPRLEATPPSFAELFRDLPPQVVSGARWGM
                     VLVVVLLLLVLVAIAVVRARRRSKKRDEDERESVWSAEAVFAGFGGMWRSLMDRLRSR
                     AQPYEEPAVGAIRALYRELLRVGSHLDIPRLGYETPYEYSPRLAQRFPERDDDVEALT
                     EIYRGLKGGLSS"
     gene            complement(1449..2717)
                     /locus_tag="AC159B_01600"
     CDS             complement(1449..2717)
                     /locus_tag="AC159B_01600"
                     /inference="COORDINATES: protein motif:HMM:NF013998.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR3376483.1"
                     /translation="MYTQGWLIVGWLLVILGLLTRRPLGFVLGAIVLFSAGASYLWDR
                     YCLWKVEYRRIFTPRRAFYGETITFTLEVTNRKLLPLAWLEVMDELPQELVPLRGRVI
                     PSLRQRRHHLVHLFSLRWYERVRRRVTMRCTARGYFALGPARLRSGDVFGVTIRGSDF
                     ERVDHLLVYPRVVPLSVLGLPALHPIGDARSRRLTFEDPTRVVGARDYLPGDPLRRIH
                     WKATARAQSLRSKQYEPTTSHRIAVFLNMDTLGRYAEYRGFVRSVLEMVILTAAAVTN
                     WAIEADYLTGLYANGYLPQGLRWVRLLPATGSEHLAAILESLAKVFPTPVMPIGDLMQ
                     LEARDLPWGTTAVVITAVTDAPLHAGILRLRDGGHTVVLVLVGDEAEEVSGVQKFRVP
                     TTRAWHALDGIDLTGFGPNSGTKGPGTRRP"
     gene            complement(2814..>3149)
                     /locus_tag="AC159B_01605"
     CDS             complement(2814..>3149)
                     /locus_tag="AC159B_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376484.1"
                     /translation="RAPTRRITSAGGKDDDDLLEPETFRRLRRRPLDDVIEEYRESSK
                     SVMRMLGKDSGVVSDIVALNLVSRYSRARLQIRKWLKEQTAAAPAAPSHLPAPALPRS
                     RTSKHRPRR"
BASE COUNT          674 a         1002 c          997 g          476 t
ORIGIN      
        1 gctcgatctc gctggcgtcg gcaacttcca aatggatcgc atcaatcacc gcgagggctt
       61 ccctcaactg cttaagccgc ctttcaaccc tcggtagatt tccgtgagcg cttcgacgtc
      121 atcatcgcgc tcaggaaatc gttgcgcgag ccgtggactg tactcatacg gcgtttcata
      181 gccaagtcgg ggaatgtcca ggtgcgatcc cacgcgaagc agctcgcgat acagtgcgcg
      241 gatcgcgccg acggccggct cctcgtatgg ttgcgcccga ctgcgcagcc ggtccatcaa
      301 tgaccgccac atccctccga atccggcgaa cactgcctcg gcagaccaaa ccgattcccg
      361 ttcatcttca tcacgtttct tcgagcgccg ccgggcacgc acaaccgcaa tcgcgacgag
      421 caccaggagg aggaggacga ccacgagcac catcccccag cgagccccgc tgacgacttg
      481 aggcggcaga tctctaaaca gttctgcgaa cgagggaggc gtcgcctcga ggcggggcgc
      541 tcgatctcta cggaacggca gctgcgagaa cacaaacaaa atcgctctgg cgatgacaag
      601 cgcgacggca aacacgatat agaagaggta ctctacgatc ggtgcaaggg gtcgaagcgc
      661 ctgcagcatg attggccgga actccacgct caccacacca gaaatcgcga cggccagcga
      721 cagaatcact cccacgacac cgatcagcaa gagaatccag tgccggttgg tcgccagggc
      781 ctgctcctcg tcggcctggg tctcctgcca gatactgatt agccgcgcga tcgcaagtga
      841 cgtcaacccg agagaaaaga acacaagcag gtagaccggc gcatgcagga tgagggcgat
      901 gccccgggtg tgcccgtaga tggcatacag cagcacgaag aacatgctcc agccaacccc
      961 gcggcggtac tgacggtcga tctcaaagtg attgaagtcg cgctccccga gcacgatgcc
     1021 ccgccaccac aacatcgtgg caatgagcgc gcccacgaca ggcggtgcga acgccgggaa
     1081 cacatcgtgc gcagcgcgga ggagcgatcc gatccagctc agatcccagg ccgcaacgtg
     1141 cggataatac gcgcgtttga ccgagaggag ccccgcgatc aggcctgcgg cagccacaac
     1201 gacccgcgcg gtggagatcg gccaggagcg gctcacggcc caccgtacgg tgatgaaccc
     1261 gccaagcaag agggcaaacg acggccacgg cccaagcagc ggcctgctgg aaccaccgta
     1321 gaacgccccg ctcagcaata gcagccaggg atacagccag cagaactcaa tgaacacgcc
     1381 ggcgaagggc agcgccgacc gcaagaaacc gcgacccgcg accatgggac cacgcgacca
     1441 gagaagggtc atggtcgcct ggtcccgggt cccttggtcc cggaattagg gccgaagccc
     1501 gtcaggtcaa ttccatccag tgcatgccag gcccgggtgg tggggacgcg gaatttctga
     1561 actcccgaga cctcctcggc ttcatcccca accagtacga ggacgaccgt atgtccacca
     1621 tcgcgcaggc gaagaatgcc cgcgtggagc ggcgcgtccg tgaccgcggt gatgaccacc
     1681 gcggttgtac cccagggtag gtcgcgcgcc tcaagttgca tgaggtcccc gataggcatg
     1741 accggtgttg gaaagacctt tgcaagcgac tccagtatgg ccgcgagatg ctcgctgccc
     1801 gtcgccggga gcagccggac ccagcgcaat ccctgcggga gatatccgtt ggcatagagg
     1861 ccggtgagat aatcggcctc aatcgcccag ttcgtcactg cagcggcggt caaaatcacc
     1921 atctcaagga ccgagcggac aaaaccgcgg tactccgcgt agcggccgag cgtgtccata
     1981 ttaaggaaga cggcgatgcg gtggctcgtc gtcggctcgt actgcttgga gcgcagcgac
     2041 tgcgcgcgcg ccgtcgcctt ccagtgaatg cggcggagtg ggtcgccagg gaggtagtcg
     2101 cgcgcaccga ccacgcgggt tggatcttcg aaggtaaggc ggcgggaacg ggcgtcgccg
     2161 atcggatgca gggcgggcag ccccagcacc gatagcggca cgacgcgcgg atagacgagt
     2221 aaatggtcga cgcgttcgaa gtcggatcct cgaatggtca cgccgaacac atcacccgag
     2281 cgcagccggg cggggcccag ggcgaagtac ccgcgtgcgg tgcagcgcat cgtgacgcgg
     2341 cgtcgtaccc gctcgtacca tctcagacta aagaggtgga ccaggtggtg gcggcgctgg
     2401 cgcagactcg ggatcacccg accacggagg ggcacgagct cctgcgggag ttcatccatc
     2461 acctcgagcc acgccagcgg cagcagtttt cgattggtga cttcgagtgt gaacgtgatg
     2521 gtctctccgt agaacgcgcg ccgcggggtg aagatgcgcc ggtactcgac tttccaaagg
     2581 caataccggt cccacagata ggaggcaccg gcgctaaaaa ggacaatcgc gccgagcacg
     2641 aacccaagcg gccggcgcgt gagaaggccg aggatcacca ggagccagcc gacgatcaac
     2701 caaccctgcg tatacacgcc agactacggt gccacgcgac caagggacca cgggaccaaa
     2761 ccgaagctat gtggtcgcct ggcagcctgg tcgcctggtc gcgtcatttg tggttaacgc
     2821 ctggggcgat gtttcgaggt tcgagagcgc ggaagcgcgg gagcagggag gtgcgatggg
     2881 gccgccggcg ctgcagcggt ttgctctttc aaccacttcc ggatctgcaa tctcgccctg
     2941 ctgtaccggg agaccaggtt caaggcgacg atgtcggaga cgacgccgct gtctttgccg
     3001 agcatgcgca tcacgctctt ggacgattcc cgatactctt caatgacatc atccagcggt
     3061 cgccgtcgaa gccggcggaa cgtctcgggt tcgagtagat catcgtcatc cttccctccg
     3121 gccgacgtga tgcggcgggt gggggcgcg
//
LOCUS       DBNGSB010000056         4641 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_1876796, whole genome shotgun sequence.
ACCESSION   DBNGSB010000056 DBNGSB010000000
VERSION     DBNGSB010000056.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4641)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4641
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_1876796"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="AC159B_01610"
     CDS             <1..912
                     /locus_tag="AC159B_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012493.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HIR3376485.1"
                     /translation="VGVFGPDTDGSGRPRVIDVFVRTNVRLVDRRGEQDRDTMLAGII
                     ARGEKTLVYTLARAHSVEMAQNLRTSTGRDVAYYHGGLPMRVREVLEQMFADGKIGVM
                     VVADGLDETAVPGDVRQVALLGLPQTAAELVEQIGTAGMDGRSSQATLLYAKLDLEAA
                     RASLAERNPTRDMLTAIYRAVREHVVKDKFASWPDPGLTEALGAVASPRVIGIGLDVL
                     AEAGVIQREYEGEHWRITLTNSSRRELSTSLRYAEGEREREALAELEHFAFGPLSEIL
                     KAVAGPTAGIRDQGTRISKTGETGKTG"
     gene            928..3120
                     /locus_tag="AC159B_01615"
     CDS             928..3120
                     /locus_tag="AC159B_01615"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIR3376486.1"
                     /translation="MKWLQARQPSAFQDLPAEVQGLVAKMRDYHPKMDLDVVRDAYLF
                     AEAAHHGQTRVSGEPYIKHSIAVATVLTDLRLDPVTVAAALLHDVAEDTGVTLDEVRE
                     KFGTEIAALVDGVTKLGKIEWMSREERQAESLRKMFLAMASEIRIVLIKLADRLHNMR
                     TIEYLPEWKQKRTSQETLDIYAPLAERLGIGQFKREFEDLAFRVLEPDAYQDLKTQIA
                     HAEPARASFVERVADILYRELNQAGVKVVRERISGRPKHFYSIWQKMKRPKYAGQPVA
                     RIYDRLGVRVILDDLKDCYAALGVVHSLWRPIPGEFDDYIANPKTSGYQSLHTTVIYE
                     GESLEIQIRTLQMHQEAEHGIAAPWTYKEGKPADREVEQKLAWLRQLLDWHSDLHDAR
                     DFVRSVKLDLFQNEVFVFTPKGDVVDLPAGATPVDFAYRIHTDVGHRTIGAKVNGRLV
                     PLSHKLQTGDIAEIVTGKTGGPSRDWLSFIITTNARTKVKQWFKREARDENIQRGREM
                     LERELRRLGALSSLMKPEKLRAIATDFEVSDEDDLLAALGNGDVSVLHVVQALRGEPV
                     EAEAPVPPPSVAPAPTRGVRVRGVENVLMRFSRCCTPLPGDRIIGYVTRGRGVTIHRA
                     DCPNTAFFKDHPERMIEVEWEVGTEGSYQVEIEVEGFDRVGLLKDVLAAIGDSKTNVV
                     TVNARVRRDKVGLINLVLDIRNVAQLHTVLQRVGKVPDVYSVERVVHS"
     gene            3117..3575
                     /locus_tag="AC159B_01620"
     CDS             3117..3575
                     /locus_tag="AC159B_01620"
                     /inference="COORDINATES: protein motif:HMM:NF019013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3376487.1"
                     /translation="MRTRKASRQSAVGSRQSTAQIKPLLDAHLQALNGRDLAKLVSFY
                     ADDAVLEFPASPPAQGISRIRQAFESFFDQWEETSAYRTVLVSGTVAAVEGTVRGRHR
                     TLHLRIPGRVAAGSRGYQHDFAMFVEFADGKIQRQRVYFDARDLVKQLLG"
     gene            3670..>4641
                     /locus_tag="AC159B_01625"
     CDS             3670..>4641
                     /locus_tag="AC159B_01625"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR3376488.1"
                     /translation="MTKQLTAARVGLFAESVIREMTRLNNLHNGVNLAQGFPDFDAPA
                     ELLEAAAKAMRDGFNQYAITWGSPRLRAAVAEKFAWYNGVEVDPDAHITVTCGATEAM
                     MASLLAIIDPGDEVIIFEPFYENYGPDALLSGAVPSYVSLNLDDPECGFDPDQLRAAF
                     TNKTKAIIINTPHNPAGKVFTRAELSLIADLCRRHGVLAVTDEVYEHLLYDGRLHVSI
                     AALEGMAERTVTINSMSKTYSVTGWRVGWAIIKNRDVTNGIRRAHDFLTVGAAAPLQE
                     AGVVALGFPREYYARTAEMYRRKRDQLLGILTDLGFRCVIPRGAYYIM"
BASE COUNT          917 a         1407 c         1497 g          820 t
ORIGIN      
        1 gtgggagtct ttggcccgga cacggacgga agcgggcgtc cgcgcgtcat cgatgtgttt
       61 gtccggacta atgtccggct ggtcgaccgc cggggcgaac aagaccggga caccatgctg
      121 gcaggcatca ttgcccgggg cgagaagacg ctcgtttata ctttggcgcg cgctcactcg
      181 gtcgaaatgg cccagaacct gagaacgtcg acaggccgcg acgtcgcgta ttaccatggc
      241 ggcttgccca tgcgggtgcg tgaggtcctg gagcagatgt tcgcggacgg gaagattggt
      301 gtcatggtcg tggccgacgg tctcgacgag actgctgttc ccggcgacgt ccggcaggtc
      361 gcccttctcg ggctgccgca aacagccgct gagctggtcg agcaaatcgg caccgcgggg
      421 atggatggcc ggtcatcaca ggcgacgttg ctttacgcca agttggatct cgaggccgcg
      481 cgcgcatccc tggccgagcg aaacccaacg cgggacatgt tgacggctat ctatcgagct
      541 gtgcgggaac acgtcgtaaa agacaagttt gcatcgtggc cggacccggg actgaccgag
      601 gcgctgggag ccgttgcctc tcctcgagtg attggcattg ggctcgatgt cctggccgag
      661 gccggggtga tccagcgcga gtatgaagga gaacactggc gcattacgct gaccaacagc
      721 agccgtcgcg aactctccac ctcactgcga tatgctgaag gtgaacgaga gcgcgaggcg
      781 ctcgcagagc tggagcattt cgctttcggc ccactctccg agatccttaa ggcagtggcg
      841 ggaccgacgg cagggatcag ggatcaggga acaaggatca gtaagactgg ggagacgggg
      901 aagactggat agacggggta gacgcggttg aagtggctgc aggcgcgaca acccagcgcg
      961 ttccaggacc tgcccgccga ggtccagggg ctagtcgcga agatgcgcga ctaccacccc
     1021 aagatggacc tcgacgtcgt ccgggacgca tatctcttcg cagaagcggc ccaccacggc
     1081 cagacccgag tctcgggtga accgtacatc aaacactcca tcgcggtggc cacggtgctg
     1141 accgacctcc gattggatcc cgtcaccgtg gcggctgctc tcctccatga cgtcgccgaa
     1201 gatacaggcg tgacgctcga tgaggtccgc gagaagttcg gaaccgaaat cgctgcgctt
     1261 gtggatgggg tcaccaaact cggcaagatc gagtggatga gccgtgaaga acgccaggcc
     1321 gagtcgctgc gcaagatgtt ccttgccatg gccagcgaga tccggatcgt attgatcaaa
     1381 ctggccgacc gcctgcacaa catgcgcacg atcgaatacc tgccggagtg gaagcagaag
     1441 cggacgtcgc aggagacgct ggacatctac gcgccgcttg cggagcgcct gggcatcggc
     1501 cagttcaagc gtgaattcga ggacctggct ttcagggttc tggagcccga cgcctaccag
     1561 gatctgaaga cgcagattgc gcacgcggag ccggcgaggg cgtcgtttgt ggagcgcgtc
     1621 gccgacatac tgtaccgaga gttgaatcag gccggggtca aggtggtccg agagcggatc
     1681 agcggccggc cgaagcactt ctacagtatc tggcagaaga tgaaacgccc caagtacgcg
     1741 gggcagccgg tcgcccgtat ctacgatcgg ctcggcgtcc gggtcatcct cgacgacctg
     1801 aaggattgct acgcggcgct cggcgtcgtg cactcgctgt ggcggccgat ccccggcgag
     1861 ttcgacgatt acattgcgaa tcccaagacc agcggttatc aatcactgca tacgacggtg
     1921 atttacgagg gagaatccct cgagatccag attcgcactc tgcagatgca ccaggaggcc
     1981 gagcacggga tcgccgcacc ctggacatat aaggaaggga aacccgccga ccgggaagtc
     2041 gagcagaaac tcgcctggct ccggcaattg ctcgactggc attccgacct tcacgacgcc
     2101 cgcgatttcg tccgatcagt caagctcgac ctgttccaga acgaagtctt cgtctttact
     2161 cccaaaggcg acgtggtgga cctgcccgcc ggtgcgacgc cggtggactt tgcgtaccgc
     2221 attcacaccg atgtcggcca ccgcacgatc ggtgcgaagg tcaacggacg cctggtgccg
     2281 ctgtcccaca aactccaaac cggcgacatc gccgagatcg tcacgggcaa gaccggcggc
     2341 ccgagccggg actggctgtc cttcatcatc acgaccaacg cccgcaccaa ggtcaaacag
     2401 tggttcaagc gagaagcgcg cgacgagaac attcagcgcg gccgcgagat gctggagcgg
     2461 gagctccgcc gtctgggtgc gctcagcagc ctgatgaagc ccgagaaact ccgggcgatc
     2521 gcaacggact ttgaggtcag tgacgaggac gatctcctcg ctgctctcgg caacggcgac
     2581 gtctcggtgc tccatgtcgt ccaggcgctc cgcggtgagc ccgtcgaggc cgaagccccc
     2641 gttccaccgc cgtcggtcgc gccggcgccg acccgcggcg tccgcgtgcg cggcgtcgag
     2701 aatgtcctga tgcgcttctc tcgctgttgc accccgctgc ccggcgaccg catcatcggc
     2761 tacgtcaccc gcggccgcgg tgtcaccatc caccgtgcag actgtccgaa cacggccttc
     2821 ttcaaggatc accccgagcg aatgatcgag gtggaatggg aggtcgggac ggagggttcg
     2881 taccaggtcg agatcgaggt cgaagggttc gaccgggtcg ggctgctcaa ggacgtgctc
     2941 gcggcgatcg gcgacagcaa gacgaacgtc gtgacggtca acgcgcgcgt gcggcgcgac
     3001 aaggtcgggc tcattaatct cgtcctcgac atccgcaacg ttgcgcagct tcacacggtg
     3061 ctccagaggg tcgggaaggt gcccgacgtg tactccgtcg agcgagtcgt tcacagttga
     3121 ggacgcggaa ggccagtagg cagtccgcgg taggcagtag gcagtccacg gcacaaatca
     3181 agccgcttct cgacgcccat ctgcaggctc tcaacggccg ggatctcgcc aaactcgtct
     3241 cgttctatgc ggacgatgct gttctggaat tcccggccag cccgcccgca caggggatca
     3301 gccgtatccg gcaggcattc gagtcttttt tcgaccagtg ggaggaaacg agcgcctacc
     3361 gaaccgtcct tgtgtcagga accgtcgccg cagttgaggg aacggtcagg gggcgccacc
     3421 ggacactgca tctgcgcatt ccgggccggg tagccgccgg gtcgcggggg taccagcatg
     3481 acttcgcaat gtttgttgaa tttgctgatg gaaagatcca gagacagcgc gtctatttcg
     3541 acgcgcgaga cctggtaaag caactactgg gataagggga taaagggaca aggggataac
     3601 gttttgcgtc gctttatccc attatcccct tatcccctta tcccgcagtt acgtacttag
     3661 gaggttagtg tgacgaagca gttgaccgcg gcccgcgttg ggttattcgc ggaatcggtc
     3721 atccgcgaga tgacccgtct gaacaatctc cacaacgggg tgaacctcgc gcagggcttt
     3781 ccggacttcg acgctcccgc ggagctgctg gaggcggcgg cgaaggcgat gcgggacggc
     3841 ttcaatcagt atgcgatcac gtggggatcg ccacgccttc gtgcggccgt cgcggaaaaa
     3901 ttcgcctggt acaacggggt cgaggtggat cccgacgccc acatcacggt tacctgtggc
     3961 gccaccgaag cgatgatggc ttcgctgctc gccatcatcg accccgggga cgaagtcatc
     4021 atcttcgagc cgttctacga gaactacggt cccgacgcgc tgctctccgg cgctgtaccg
     4081 tcctacgtgt ccctgaatct cgatgatccg gagtgcggct tcgatccgga ccagctgcga
     4141 gctgcattca cgaacaagac gaaggcgatc atcatcaaca cgccccacaa tccggccggg
     4201 aaggtcttca cacgtgcgga gctctcactc attgccgacc tgtgccggcg gcacggtgtc
     4261 ctcgccgtga cggacgaggt ctacgagcat ctgctgtacg atgggcggct gcacgtcagt
     4321 attgccgcac tcgagggcat ggcggagcgc acggtgacga tcaatagtat gagcaagaca
     4381 tacagtgtca cgggctggcg agtaggctgg gcgatcatca agaaccggga cgtgacgaac
     4441 ggcatccgcc gcgcccacga cttcctgact gttggcgcag cggccccgtt gcaggaagcg
     4501 ggtgtggtcg ctctcggctt cccgagagag tattatgcgc gtaccgccga gatgtaccgg
     4561 cgcaagcgcg atcagctgct cggaatcctg accgatctcg ggttccggtg cgtgattcct
     4621 cgaggcgcgt attacatcat g
//
LOCUS       DBNGSB010000057         6600 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3411779, whole genome shotgun sequence.
ACCESSION   DBNGSB010000057 DBNGSB010000000
VERSION     DBNGSB010000057.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 6600)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6600)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6600
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3411779"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..2530)
                     /locus_tag="AC159B_01630"
     CDS             complement(134..2530)
                     /locus_tag="AC159B_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003349489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HIR3376489.1"
                     /translation="MMENRRLYRAAVLGSGVMGGAIAAHLANAGVPVLLLDVVPSSLT
                     DDERRRGLTLTDPLVRSRISTTGKERALTANPASFFTPARAQLISTGNFEDDLPKIRE
                     ADWIIEAIVEDLAVKQQLLEQVERHRRDDAIVSTNTSGLPVHQIASGRSERFRRSFLG
                     THFFNPPRYLKLLEIIPLPETDPDVVAAARRWGEWRLGKGVVYAHDRPNFIANRIGAY
                     ASRSVVHLMLEMGLTVEEVDELTGPLIGRPRSATLRTLDLVGLDTAILVTDNAYRNLA
                     DDEERDVFIVSPLMGEMAKRGWLGEKSGGGFYKRVNGEIHALDYKTMEYRPRQKVAMA
                     SVESAKLVEDPQKRLRQLLTAPDKAGQFLWRLTSSVLVYAAKRIPEISDDIVNVDRAM
                     RWGFAWEHGPFETWDLLGVSDVGKRLQEEGREVPPLVRAVMDAGGKFYREHDDGRREF
                     FDLQTRAYQPELERPGVIVLSRLKKSNRVIATNPGASLVDLGDGIACLEFHSKLNTIG
                     EDTMRMVYRALDETRKKLNGLVIGNQGQEFSAGANLMLLLLEAQEGNWDELALVIRQF
                     QKANLAVRYFELPVVAAPFGRTLAGGCEVCLPCARMQAAAETYIGLVETGVGLIPAGG
                     GTTEMVRRAAARVPDGDGDLFPFARWVFETIALAKVSTSAEEAYTLGYLREGDAISMH
                     SDHLIHDAKEACLALVRTGYRPPLKIPVRVTGERGLSAFESYLYLMKTAGNISAHDVV
                     VGTKLANVVCGGRVPYATEVTEEYLHDVEREAFLSLLGQPKTLERIRHTLKTGRPLRN
                     "
     gene            complement(2623..4980)
                     /locus_tag="AC159B_01635"
     CDS             complement(2623..4980)
                     /locus_tag="AC159B_01635"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HIR3376490.1"
                     /translation="MNSTRRLLRVVLFLFALAAIVVAGLGVYVIRRPFPVVRGQLQIP
                     GVRAAVEIIRDRWGIPHIFAQNMPDLILAQGYVHAQDRLWHMEFNRRIASGRLSEIFG
                     EVTLKEDRFLRTVGIRRSAELDLAQLDPETRSALESYAAGVNAFITQHRRRLPVEFTV
                     LRFSPEPWTPVDTLAFAKLMAWVLGGNWESEILRAHILSRFGPATAHVLLPPYPGHHP
                     VIVDPAVSNAFRSSAILKVLDVAPRAAGVGSNNWVLGGTRTSTGRPILANDPHLEAQM
                     PSIWYEMHLSGGRYDAIGSTFPGTPGVVIGHNADIAWGVTNAGPDVMDLYVERFDAAD
                     PTRYEYRGRWERAQVVREEIKVKGRDDPVVETVRITRHGPIINNVEEGLNAFLALRWT
                     GLETSSVAGSVLRIGRARNWDEFRDALRLWTIPAQNFVYADREGNIGYQLPGRIPIRA
                     TGRGLVPVPGWTGEHEWTGEIPFDDLPTMLNPPRGYVVTANNQIVPDRYRHFISSEWD
                     PGYRARRIEALLAGPGLDLEHMRKVQADLTSIPGQVLVGALHGVRAPTDPAAGLLREL
                     LVWDGVVRPDSSAAAIYEALRITLLEEIFAPVLGKDLFERYRRHAQFWQIALLRIVQD
                     PTSPWWGPEGRDALVTRALTKTARTLTEKLGSDRTKWQWGRLHVMRFMHPIGRVPGLG
                     WIFNATSPPTGGDGFTVNNAGFNALNFRQVVVASYRQIIDVADWDRSISIHTTGQSGL
                     PFHKHYRDFVPLWASGRYHPMLFTRARIEQVAAGKLTLTPAQPAE"
     gene            5112..6008
                     /locus_tag="AC159B_01640"
     CDS             5112..6008
                     /locus_tag="AC159B_01640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR3376491.1"
                     /translation="MPQVRAAVVSVAAGIGVLVFKFTAYLLTGSVAILSDAAESVVNV
                     VAANIALISLVVAVRPPDRGHPYGHGKAEYLSSATEGWLILLAGGWTFFTAAQRFVRP
                     LPLRNLETGLLVLAVATLANYLTARFLLRVSREAQSIALEADARHLLVDVITTFGVFA
                     GLGLVRFTGIQRLDAVVGVLIGLHILSMGFGIIRRSVGGLMDTNLPPDEESKVRVILG
                     DHKEEIVSYHGLRSRQAGSDRFIDLHLVLHRTLSVGEAHTMCDDLEDHIREALPGADI
                     TIHVEPCGPDCRRCAAILAARL"
     gene            6097..>6600
                     /locus_tag="AC159B_01645"
     CDS             6097..>6600
                     /locus_tag="AC159B_01645"
                     /inference="COORDINATES: protein motif:HMM:NF019352.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HIR3376492.1"
                     /translation="MRKKAISRRDFLKATAGAAGILGGLAVPGGKALAAGRSHGQSHS
                     AAAPVYPTTAQHGGNITVGEVDVARMGYDPMQFLTHFDYGKVSKLPNGQTLREFRLVA
                     DEGREVEIAPGVFFPAWTFNGTVPAPTLRAREGDRVKVTLINASSHPHTIHFHGFHKA
                     EMDGVPGI"
BASE COUNT         1178 a         2108 c         2037 g         1277 t
ORIGIN      
        1 tgcctctttc atcgttcaca ctccctcttg cgtacgcacc gtggggccgg agactggaac
       61 aaaggcgacc cgtaactaag tcactcctca gccgatttgc cgggtcgccg gttccggctc
      121 cggccccgcg ggactagttt cgcaaaggcc ttcccgtctt cagtgtatgc cgaatccgct
      181 ccagtgtctt cggctgtccg agcaggctca agaatgcttc ccgctccaca tcgtgcagat
      241 attcctccgt gacctcagtg gcatagggca ctcgtccacc gcacacgacg ttcgcaagct
      301 tggttcccac gaccacatcg tgcgcggaaa tattgccggc ggttttcatg agatagaggt
      361 agctttcgaa tgcgctcagc ccacgctcgc ccgtcaccct caccgggatc ttgaggggag
      421 gccggtagcc ggtccgtaca agtgcgaggc acgcttcttt ggcatcgtgg atcaagtggt
      481 cgctgtgcat cgagatggcg tcgccctcgc ggagataccc cagcgtatag gcttcctctg
      541 ccgacgtgga cactttcgcg agtgcgatcg tctcgaagac ccagcgtgca aacggaaaga
      601 gatcgccgtc cccgtccggg acgcgcgccg ccgcgcgccg gaccatttcc gtcgtgcccc
      661 cgcccgccgg gatcaggccg acgccggttt cgaccagccc gatgtacgtc tccgccgcgg
      721 cctgcatccg cgcgcacgga aggcagacct cgcacccgcc cgcgagcgtg cgtccaaatg
      781 gcgcggcgac gacgggcagc tcgaagtagc gcactgcaag atttgccttt tggaactgcc
      841 ggatcacgag ggccagttcg tcccagttcc cctcctgcgc ttccaatagc agcagcatca
      901 agttggcgcc tgcggaaaat tcctgaccct ggttgccgat cacgagcccg ttcagcttct
      961 tacgggtttc gtcgagggct cgatagacca tccgcatcgt atcttcgccg atcgtgttga
     1021 gcttcgagtg aaactccaga caagcgatcc cgtctccgag atccacgagg ctggcgccgg
     1081 ggttagtggc gatgacgcgg ttcgacttct tgagtcgcga gaggacgatg acgccgggcc
     1141 gctctagctc cggctgatac gcacgggtct ggaggtcgaa aaattctcgc cgtccgtcat
     1201 catgctcccg gtagaatttc cctccggcgt ccattactgc ccgcaccaac ggcggaacct
     1261 cgcgaccttc ctcctgcaaa cgtttgccca cgtcagaaac gccgagaagg tcccacgttt
     1321 cgaacggtcc gtgttcccag gcaaagcccc atcgcatcgc gcggtcgacg ttcacgatgt
     1381 cgtcggagat ctccgggatg cgcttcgccg cgtacacgag cacgctgctc gtgaggcgcc
     1441 agaggaactg ccccgctttg tcaggcgccg tgagcagctg cctcaggcgt ttttggggat
     1501 cttcgacgag cttcgcgctt tcgacgctgg ccatcgcgac tttctgccgc ggacggtact
     1561 ccattgtctt gtagtcgaga gcgtgaatct ccccgttcac gcgcttgtaa aagcctccac
     1621 cgcttttctc acccagccat ccgcgcttgg ccatctcgcc catcagcgga gagacgatga
     1681 agacatcgcg ctcctcgtca tcggcaagat ttcgataggc gttatccgtc acgaggatgg
     1741 cggtatccag gccgacgagg tccagcgtgc ggagtgtggc gctgcgtggg cggccgatga
     1801 gtgggccggt gagctcgtcc acttcctcca cggtcagtcc catttcgagc atcaggtgca
     1861 cgaccgagcg cgaggcgtag gcgccgatcc gattggcgat gaagttcggc cggtcgtgcg
     1921 cgtacacgac gcctttcccg agtcgccatt caccccagcg ccgcgccgcc gccaccacat
     1981 cgggatcagt ttccggaagc gggatgatct ccagcaattt cagataacgg ggagggttga
     2041 agaagtgcgt gccgaggaag ctccgccgga accgctctga gcggccggaa gcgatttggt
     2101 gcactggcag gccgctcgtg ttggtgctga cgatggcgtc gtcgcgccgg tgacgttcga
     2161 cctgctcgag gagttgctgc ttgaccgcca gatcctcgac gatcgcctca atgatccagt
     2221 ctgcctcacg gatcttcgga aggtcgtcct cgaagttacc ggtggagatc agctgcgccc
     2281 gcgccggcgt gaagaacgag gcggggttcg ctgtgagggc gcgttctttt ccggtggtgg
     2341 agatgcggct gcgtacgagt ggatcagtga gtgttagccc gcgcctgcgt tcatcgtcgg
     2401 tcaggctcga cggtactacg tcgagcagca ggaccggcac gccggcgttt gcgagatggg
     2461 cggcgatcgc gccgcccatc acacctgagc cgaggactgc ggctctatag agtcggcgat
     2521 tctccatcat atctacctat ggggcctggc cccaaggtgc caggccccaa ggtgccaggc
     2581 ccctttgctg cacgtcttgg ccaggcacga ttagctcacg cgtcattccg caggttgcgc
     2641 gggagtcagc gtgagctttc ccgcggcgac ctgctcgatt cgggccctgg tgaagagcat
     2701 cgggtgatag cggcctgacg cccagagcgg cacgaagtcc cggtagtgct tgtgaaacgg
     2761 cagcccgctc tggcctgtcg tgtggataga gatcgagcga tcccagtcgg cgacgtcgat
     2821 gatctggcga tacgaggcaa cgacgacctg cctgaaattc aatgcgttga aacccgcgtt
     2881 gttcacggta aacccgtcac cgccggtcgg cggtgaggtc gcgttgaaga tccagcccaa
     2941 gcccgggacg cggccgatcg ggtgcatgaa ccgcatcacg tgcagccggc cccactgcca
     3001 tttggtccgg tccgatccga gcttctctgt gagggtgcgt gcggttttgg taagcgctct
     3061 cgtcaccagc gcatcacgtc cctcgggccc ccaccatgga gaggtcgggt cttgcacgat
     3121 tcgcagcagc gcgatctgcc agaattgcgc gtgccgccgg tatcgctcga aaagatcctt
     3181 ccccagcact ggagcgaaaa tctcctcgag cagtgtgatg cggagcgctt cgtagatggc
     3241 cgcggcactg ctgtcggggc gcacgacgcc atcccacacc aggagttccc gcagcagacc
     3301 cgccgcgggg tcagtgggcg cccgcacacc atgtaatgcc ccgaccaata cttgaccggg
     3361 gatcgacgtg aggtctgctt gcaccttccg catgtgctcc agatcgagcc ccggccccgc
     3421 cagcaacgct tcgatcctcc gggcccgata gccgggatcc cattcagagg aaatgaagtg
     3481 ccggtagcgg tccggaacaa tctggttgtt ggcggtgacg acgtacccgc gcggcgggtt
     3541 gagcattgtc gggaggtcgt caaaaggaat ctcaccggtc cactcgtgtt cccccgtcca
     3601 gcccggcacc gggacgagac cgcgccctgt ggcgcggatg gggatccgac cgggcagctg
     3661 ataaccgatg ttcccttccc gatcggcata gacaaagttc tgtgcgggga tcgtccagag
     3721 gcggagcgcg tcgcgaaact catcccagtt gcgcgcacgg ccgatgcgca ggaccgaacc
     3781 ggcgaccgag ctggtttcca ggcccgtcca gcgcagcgcc aggaacgcat tcaatccttc
     3841 ctcgacgttg ttgatgatcg gtccgtgccg ggtgatgcgt accgtctcga caactgggtc
     3901 atcccggcct ttgaccttga tttcttcccg cacgacctgc gcccgttccc agcgcccgcg
     3961 gtattcgtag cgcgtcgggt ccgcggcatc gaagcgctcg acatacaggt ccatcacatc
     4021 gggaccggca ttggtcacac cccaggcgat gtccgcgttg tgtccgatga ccacccccgg
     4081 cgttccgggg aacgtgctgc cgatagcgtc gtaccgtccc cccgagagat gcatttcgta
     4141 ccagatcgag ggcatctgcg cttccagatg aggatcgttg gccaggatcg gcctgcccgt
     4201 cgacgtgcga gtcccgccga ggacccagtt gttgctcccc acgcccgctg cacgcggcgc
     4261 gacgtcgaga actttcagga tggcgctgga gcggaatgcg ttcgacacgg ccggatcaac
     4321 gatcaccgga tgatggcctg gatagggagg cagcaacacg tgcgcggtgg caggtccaaa
     4381 ccggctcaaa atgtgcgcgc gaaggatttc tgattcccag ttcccgccca gcacccaggc
     4441 catcagcttt gcgaaggcaa gcgtgtcgac cggcgtccac ggctccggag agaaacgcag
     4501 cacggtgaac tcgaccggta gccggcgccg gtgctgagtg atgaacgcgt tgacgccggc
     4561 cgcataggat tcgagagcgc ttcgagtctc cggatcgagt tgcgcgaggt cgagctcggc
     4621 actgcgacgg atgcccacag tgcggaggaa acggtcttcc ttaagtgtga cttctccaaa
     4681 aatctctgag aggcgcccgc tggcaatccg cctgttgaac tccatgtgcc acagccggtc
     4741 ttgcgcgtga acgtaccctt gagcgaggat caggtccggc atattttgcg caaagatgtg
     4801 gggaatgccc cagcggtcgc ggatgatctc aaccgctgcg cgcacgcccg gtatttgcag
     4861 ctgcccccgc acgactggga agggacggcg gatcacatac acgccgaggc cggcgacgac
     4921 aatcgccgca agcgcgaata agaacagcac cacccgcaag agccgtcgcg tagagttcac
     4981 ctcacgcaaa ggaaaatgga aaagggaata tggaaaatgg accgatgacc taattttcgc
     5041 ctcccttttc cctcttccct attccttttt gctacactcg ggccgtgctg cgttctgatc
     5101 gggtcatccg tatgccccag gtccgtgccg cggtcgtctc cgttgccgcg gggatcggcg
     5161 tgcttgtgtt caagtttacc gcctatctgc tcaccggttc agtggccatc ctgtccgacg
     5221 ccgcggaatc cgtcgtcaac gtcgtcgccg cgaacattgc gctgatcagc cttgtcgtcg
     5281 cggtccggcc gcccgaccga ggtcatccct acgggcacgg aaaggccgag tatctcagca
     5341 gcgcgacaga gggatggttg atcctgctcg cgggcggctg gactttcttc actgccgcgc
     5401 aacgctttgt acgcccgctg ccgctgcgca acctggagac ggggctcctc gtgctcgctg
     5461 ttgccacgct tgcgaactat ttgaccgcgc gattcttact ccgtgtgtcg agggaggcgc
     5521 agtcgatcgc gctcgaggcc gacgccagac acctgctcgt cgatgtgatc accacattcg
     5581 gagtcttcgc cggcctgggg cttgtccggt tcaccgggat ccagcggctg gatgccgtag
     5641 tcggcgtgct cattgggctc cacatccttt cgatggggtt cggcataatc cgacgatcgg
     5701 tcggcgggct gatggacacg aacctgcctc ccgatgagga atcgaaggtg cgcgtgatcc
     5761 tcggcgatca caaagaagag atcgtgagct accacggact gcggtcacgc caagccggct
     5821 cagaccgctt catcgacctt catcttgtgt tgcaccgtac gctctcggtc ggcgaggcac
     5881 acacgatgtg cgatgatctc gaggatcaca tccgcgaagc gctgccgggg gccgacatta
     5941 ccatccacgt agaaccctgc ggaccggact gcagacgctg cgccgccatc ctcgccgccc
     6001 gattgtagtc cctgcttgac tcaaaccagg cttcctgggt acgatttagg tgaggctaat
     6061 ttagcctggt ctaaattcac tttggggtgg actcatatgc ggaagaaggc catttccagg
     6121 cgagacttct tgaaggcaac agccggggcg gccgggattc tgggcggact tgccgttcca
     6181 gggggaaagg ctctcgcggc cggccggtcc cacggacagt cgcatagcgc ggcagcgcct
     6241 gtgtacccga cgactgcgca gcacggcggt aacatcaccg tgggtgaagt tgacgtcgcg
     6301 cggatgggat atgatccgat gcagttcctc acccatttcg attacgggaa agtgagtaag
     6361 ctcccaaacg gccagacgct ccgagagttc cgtcttgtcg ccgatgaggg acgcgaggtc
     6421 gagatcgcac ccggggtctt ctttcctgcg tggacgttca acggtacggt gccggcgccg
     6481 acgctccggg cgcgcgaagg tgatcgggtc aaggtgaccc tgatcaacgc cagctcgcac
     6541 ccgcacacga tccatttcca cggattccac aaagccgaga tggacggcgt gcccggcatc
//
LOCUS       DBNGSB010000058         4514 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_4025028, whole genome shotgun sequence.
ACCESSION   DBNGSB010000058 DBNGSB010000000
VERSION     DBNGSB010000058.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4514)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_4025028"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            287..436
                     /locus_tag="AC159B_01650"
                     /pseudo
     CDS             287..436
                     /locus_tag="AC159B_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536826.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            719..4246
                     /locus_tag="AC159B_01655"
     CDS             719..4246
                     /locus_tag="AC159B_01655"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HIR3376493.1"
                     /translation="MNSSRIESLLVANRSEIAIRVMRAASEMGIRTVGIYSREDRLAL
                     HRFKSDESYVVGEGKKPLAAYLDVDDILRVARQTGVDAIHPGYGFLSENPEFAQAVID
                     AGIAWIGPSPDVMRTLGNKVAARNAAAAAGVPVMPATAPLPADLAQCKQLAAEVGYPL
                     MLKASWGGGGRGMRVIESPDDLDTLLPAARREALAAFGNDEVYFEKLVRRARHVEVQV
                     LADRHGRIVHLYERDCTVQRRNQKVVERAPAPYLDEQTRRALCEDALKLMRAVGYSHA
                     GTVEFLMDADTGQFYFIEVNPRIQVEHTVTEMVTGIDIVKAQIRITENGHLGLVEDEF
                     DVGGQLVRRGTGVPAQDDIALNGHALQCRVTTENPESGFLPDSGRLAEYRSAAGFGVR
                     LDAGTAYGGAVITPYYDSLLVKVTTWALTAGETIQRMDRALREFRIRGIASNLQFLEN
                     VINHPSFAAGDVTTRFIDTTPELLAFTQKRDRATKLLSYIGEVTVHGHPEMKGRRLPE
                     RPLPKPVLPPVDGRSVAPRGTRDLLLDLGADRFAQWMLEQQRVLLTDTTMRDAHQSLF
                     ATRMRTADMLPIAPYYARALSGLFSLECWGGATFDVALRFLKEDPWERLAQLREGIPN
                     MLFQMLLRGSNAVGYTNYADNVVRFFVRQAAAGGVDLFRVFDSLNWVENMRVAIDAVC
                     ESGALCEGAICYTGDLFDDARSKYDLKYYVRIAKELEAASVHILAIKDMAGVCRPRAA
                     SVLVKALKEETGLPLHFHTHDTSGISAATVLAAIDAGCDAVDGALDAMSGLTSQPNLS
                     SIAAALAGTERDPQVSQDSLHTASTYWEGVRRFYAPFESDIRSGTADVYRHEMPGGQY
                     TNLREQARGLGLAHRWTEVSQAYADVNRLFGDIVKVTPTSKVVGDMALMLVASDLTAA
                     DVMDPAREIPFPESVVSLFKGDIGFPPDGFPQELSRKVLRAAPPLPVRPGSRIAPVDL
                     EAARAEAELRCGAELDDRRLASYLMYPKLTQDCFAHRQRFGDTSQLATPTFFYGMQPQ
                     EEVAIDIEPGKTLLVVLQTVQKDSDGIRLQYELNGQSRIVHVDDRSVAAESDAAESKR
                     RKAEPGNPLHVAAPMPGAVVSVAVEPGQKVQAGSTLLALEAMKMETHIAAERDCEIES
                     VLVKPGMRVQAKDLLIALRDIRLNSNKGT"
BASE COUNT          844 a         1393 c         1448 g          829 t
ORIGIN      
        1 cgcggcccgt cggtgacgaa catccataac ccagacgcag cacggcaaca gcgtcacgta
       61 ttccagattt tcgcgatgcg cggtcagcca gctgcatcgg ccgacagccc accagttggt
      121 ctgcctgccg aaagtcgaat taacccggag atgtaattga cgcttcctcc cgccgagagg
      181 ggaattgagg ggcgaatgaa agcgaggagc cctcgctgta cctgacggca tcgaagtgcc
      241 aaagtggtgg ttctgcaacg acctcaaacg gtaaggaggg ctcgagatga gggttacgac
      301 atttgggctg gacttggcaa agcgggtgtt tcaagtgcac tgggtggata tggaaacagg
      361 cgagatctgc cgacggcaat tgaagcggga tcaagtggcg gagttcttcg ccaagcgaga
      421 gtcttcggtg ggcgcctgag tctgattcat ccagctattc aaggtgagag acaaacgtga
      481 ataaactacg ccgttccatt gccacgctcg ccgcccccgg tattcctgta cctgcgacgc
      541 ggtctgacca tcagccaagc ccaccaggtc tgggtgacgg acatctccta cgtgccgatg
      601 gcgtgtggct tcgtgttcct caccgccgtg ctcgactagc cggagagttc cccaggcggc
      661 gtgcgccctt ggttctttac atcgcgccca tccccgtcca cgggggtgct ccattcgaat
      721 gaacagcagc cgcattgagt cccttctcgt tgccaatcga tcggagatcg ccatccgcgt
      781 gatgcgcgcg gcttccgaga tgggcattcg cacggtcggc atctactccc gcgaagatcg
      841 tctggcgctc caccgcttca agtcagacga aagctacgtt gtcggtgaag ggaagaagcc
      901 gcttgccgcc tacctcgatg tcgacgacat cctccgcgtc gcgcgccaga ccggcgttga
      961 tgcgattcac ccggggtacg gtttcctttc ggagaacccg gagttcgccc aggcagtgat
     1021 tgatgccggc atcgcatgga tcggtccgtc gcccgatgtg atgcgaacgc tcggcaacaa
     1081 ggtggccgcg cgcaacgccg cggccgccgc cggcgtgccg gtcatgcccg cgacggcgcc
     1141 gttgccggcg gacctggcgc aatgcaagca gctggcggcg gaggtcggtt atccgttgat
     1201 gctgaaggcc agctggggcg gcggtgggcg cggcatgcgg gtgatcgagt cgcccgacga
     1261 cctggacact ctgttgcccg ctgcacggcg cgaagcgctg gctgcgttcg ggaacgacga
     1321 ggtctatttc gaaaagctcg tgcgccgcgc gcggcacgtc gaagtgcagg tgctggccga
     1381 tcgccacggg cgcatcgtgc atctatacga acgcgattgc acggtgcagc ggcgcaacca
     1441 gaaggtagtc gagcgcgcac cagcgccgta cctggatgag cagacgcgcc gggcattgtg
     1501 cgaagacgcg ctgaagctga tgcgcgccgt cggttacagc catgcgggca ccgtcgagtt
     1561 cctgatggat gccgacacgg ggcagttcta tttcatagaa gtcaacccgc gcattcaggt
     1621 cgaacacacg gtgaccgaga tggtcaccgg aatcgacatc gtcaaggccc agatccgcat
     1681 caccgagaac ggccacctcg gtctggtcga ggacgagttc gatgtcgggg ggcaactcgt
     1741 gcgccgcggc acgggcgttc cggcgcagga tgacatcgcg ctcaacggcc atgccctgca
     1801 atgccgagtc acgacggaaa acccggaaag cggtttcctg cccgactctg gacgccttgc
     1861 cgaataccgc agcgccgcgg gcttcggagt gcggctcgat gcggggaccg cgtatggcgg
     1921 cgccgtcatc accccgtatt acgactcgct gctggtcaag gtcacgacat gggcgttgac
     1981 cgcgggcgaa actatccagc gcatggaccg ggcgctgagg gagttccgca tccgcggcat
     2041 cgcctccaac ctgcagttcc tggagaacgt gatcaaccac ccgtcgtttg cggccgggga
     2101 cgtcacgact cgattcatcg acacgacgcc ggaactgctg gcgttcacgc agaagcgtga
     2161 ccgcgctacc aagctcctga gctacatcgg tgaagtgacg gtccatgggc atcccgaaat
     2221 gaaggggcgg cgactgccgg agcgtccgct gcccaagccc gtcctgccgc ccgtcgatgg
     2281 gcgttccgtt gctccgcgcg ggacgcgcga ccttttgctc gacctcggcg cggaccgctt
     2341 cgcgcagtgg atgctcgagc agcagcgggt actgctgacg gacaccacga tgcgcgacgc
     2401 gcatcagtcg ctgttcgcga cccgcatgcg cacggcggac atgctgccga tcgcgcctta
     2461 ctacgcgcgg gcgttgtccg gtctcttttc gctcgagtgc tggggtggcg cgaccttcga
     2521 cgtcgcgttg cggtttctga aggaggaccc ttgggagcga ctggcccagt tgcgggaagg
     2581 gatcccgaac atgctgttcc agatgctgct gcgcggctcc aacgcggtcg gctataccaa
     2641 ctatgctgac aacgtggtcc ggttcttcgt ccggcaggcg gcggcaggcg gggtcgactt
     2701 gttccgcgtg ttcgactcct tgaactgggt ggagaacatg cgcgtggcga tcgacgcggt
     2761 gtgtgaatca ggcgcgttgt gcgaaggcgc catctgctac acgggtgatc tgttcgacga
     2821 tgcccgatcg aagtacgacc tgaagtacta cgtccgaatc gcgaaagagc tcgaggcggc
     2881 aagcgttcac atcctcgcca tcaaggacat ggccggcgtc tgccgcccac gtgcggcgtc
     2941 cgtccttgtc aaggcactca aggaagaaac cggactgccg ctgcacttcc atacgcacga
     3001 cacgagcggc atctcggctg ccaccgtgct cgcggccatc gatgcgggat gcgatgctgt
     3061 ggacggtgcg ctcgatgcga tgagcggcct gacgtcgcag ccgaacctct ccagcatcgc
     3121 cgctgcactg gcgggcaccg agcgcgatcc gcaagtaagc caggactcgc tgcacaccgc
     3181 atcgacctac tgggagggcg tgcggcgctt ctatgcgccg ttcgaaagcg acatccgttc
     3241 cggcacggca gacgtctacc ggcacgagat gccgggcggc cagtacacga acctgcgcga
     3301 gcaggcgcga gggcttggtc tcgcgcatcg ctggacggaa gtctctcagg cgtacgccga
     3361 cgtgaaccgg ctcttcggcg acatcgtcaa ggtgacgccc acgtccaagg tcgtgggcga
     3421 catggcgctg atgctggtcg ccagcgattt gaccgcggcc gacgtgatgg atccagcgcg
     3481 ggagatcccg ttcccggagt ccgtcgtgtc gctcttcaag ggagatatcg gatttccgcc
     3541 ggacgggttc ccgcaggaac tctcacgcaa ggtgttgcgc gctgcgccgc ccctgccggt
     3601 caggccgggc tcccggatcg cgccggtcga tctcgaggcc gcgcgcgccg aagcggagct
     3661 gcggtgcggc gcagagttgg acgaccgccg tctggcgagc tatctgatgt accccaagct
     3721 aacgcaggac tgcttcgcgc accgacaacg gttcggcgac acatcgcaac tggccacgcc
     3781 gaccttcttc tatgggatgc aaccacagga ggaagtggcg attgacatcg agcccggcaa
     3841 gaccttgctc gtcgtcctgc aaacggttca gaaggattcg gacggcattc gacttcagta
     3901 cgagttgaat ggccaatccc gcatcgtgca cgtcgatgac cggtccgtag cggccgaatc
     3961 cgacgccgcc gagtcgaaac ggcgaaaagc cgagccgggt aacccgttgc atgtcgctgc
     4021 cccaatgcca ggcgccgtcg tgtccgtggc cgtcgagccg ggtcagaagg ttcaggccgg
     4081 aagcactttg ctggcgctcg aggcgatgaa gatggaaacg cacattgctg ccgagcgcga
     4141 ctgcgaaatc gagagcgtgc tggtgaagcc gggcatgcgc gtccaggcca aggatctgct
     4201 gatcgccctt cgtgacatca ggttaaattc caacaaaggt acgtaaactc tcacagcggt
     4261 caagcggtgc tgccactccg aggtcgcttc aatccgtgct ttttgagcca ttgatgcaga
     4321 ttcgcctcgg tggtgtcata aagtatcctt tgatgtgttc atctttttgc atcttatttg
     4381 gaacgctatt ccgatgaaat tgaccgtgac atagcacatg cacgactcag ttaaccggga
     4441 actcatctcg cggcacaaac gcctgaaagc gttttccgtg gacgacttcg atctactctg
     4501 cgaagtcatc gcat
//
LOCUS       DBNGSB010000059         5414 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_804994, whole genome shotgun sequence.
ACCESSION   DBNGSB010000059 DBNGSB010000000
VERSION     DBNGSB010000059.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5414)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_804994"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="AC159B_01660"
     CDS             complement(<1..431)
                     /locus_tag="AC159B_01660"
                     /inference="COORDINATES: protein motif:HMM:NF013595.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HIR3376494.1"
                     /translation="MRRLLVILLGLAAVGCNGVGLISPIPTGPRIYIADRDNHRIVRV
                     NDMTGSGWVAFGTLGGGTNQFNQPAGIHVDSSGRIYVGERSNHRIVRMNDMAGVAWTS
                     FGSLGSGTDQFNRPRGVFVDGTGRIYVADVFNNRIIRIDSIT"
     gene            556..2511
                     /locus_tag="AC159B_01665"
     CDS             556..2511
                     /locus_tag="AC159B_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR3376495.1"
                     /translation="MAQTVGDSLEAGRKAIKGHAWREAFELLGAADPSTLTAQDYEHL
                     AEAAWWTGRLDACIDARERAYNIYLQQGNQRRAGSMALELAHDHEFKRAPPIAAGWQS
                     KGERLLEQEPEGPEHGVLSRRRTNAAMGKGDFESALAHAKRTVDIGMKFGDRGLQGMG
                     LHDQGRVLIAMGRADEGMALIDEATVAAVSGELQPLPTAVIYCNTIGTCRDMVDYQRA
                     GQWSEAAKRWCDRQHITGFPGMCRVYRAEIMRLRGAWTEAEQEAKRACEELKDFCLDY
                     AAGAFYEVGEIRLRVGDFQAAEAAFNHAHEIGKDPQPGLALLRLAEGKIAGAQASIRR
                     ALEETHDRLARVRLLPAEAEVALAAGEIESARSAVEELEAIAKDYSTPALLAYASYCR
                     GALQLATGNTVQARDSLRQSIRLWQDIESPYEMARARLLLSTTYRAMGDMEDAGLELH
                     AARSVFERLGAIPDLRRADSLLKEEGAAGRSVPTGRQASRTFMFTDVVRSTPLVEAIG
                     DAAWADVVRWHDQTLRSLFATHEGEEADHAGDGFLVAFNDSTTAVECAVAIQRALAEH
                     RHTHGFAPQVRIGIHTSQAVQEGGKYKGKGVHEAARIGALAEGGEIVVSGDTLAGSPP
                     RFPAINPRTVTLKGLSQSVTVCSLDWR"
     gene            complement(2551..2964)
                     /locus_tag="AC159B_01670"
     CDS             complement(2551..2964)
                     /locus_tag="AC159B_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376496.1"
                     /translation="MADKVRRVEYFYATVPDKPGEGIRVLSALKDAGVLLLAFHSFPT
                     SGGQAQVDFVPENAGKFKQAAQSARVTLTGPKSAFLITGDDRVGAAAEHAKKLADAKV
                     NISAVTAVGAGSGRYGMLLWVAPADFEKAAKALGA"
     gene            3175..3963
                     /locus_tag="AC159B_01675"
     CDS             3175..3963
                     /locus_tag="AC159B_01675"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR3376497.1"
                     /translation="MLSTARRAIGAALLILALVLAFELPVSAAKKQQQPRLTRQQLIA
                     LVNKYAAKYGIPQAIAHNLVEVESGWRQNAVSYRGARGIMQLMPMTARDLGVNRRDPK
                     QNIEGGMRYLRQLYNRFRSWDLALAAYHSGPRNVIRHKGIPPVSRSYVRRILRNSGSV
                     AYASPARRAAKAAPATVPTPGFEKRQSVTVSGQTTTTVRESLRDGQVVWRAEDIVTVQ
                     GNVRINMTREYRLVDGTLTLVKEQTLVYTITDESITESVPNEEK"
     gene            complement(3964..>5414)
                     /locus_tag="AC159B_01680"
     CDS             complement(3964..>5414)
                     /locus_tag="AC159B_01680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR3376498.1"
                     /translation="VFTTLDLDMQIAAELAVREGIDEAQRQKLNVTQGALVAIEPRTG
                     YVRAMVGGYDFNQSQFNRAWQARRQPGSAFKPFIYVTALANGMPPTKIIEDVPVEYFV
                     TDAKPEFQHWQPKNYDEKFRGPVTMRYALENSINIPAVKTIAELGAVRVIEYARRMGI
                     KSPLQPNLSLALGSNDVTPLEMTSAYGTLAGLGVRTEPIPIKKIQARGGQVLEEHEPR
                     QELALSADVAYVMTDLLKGVIARGTGRGAALGRPVAGKTGTTDDYRNAWFVGYTPSLV
                     TTVWVGNDDNSPMKKVVGATVPTRIWASFMVPATQNLPPDDWTMPAGVSERMVCGTSG
                     LLAAGDCTDPRREIFIEGTEPTEYASGAGKPGSPGVIPPQQNPVLPDQPVRSPLPLNV
                     TAPANGVALTTPFTIEGTTTPGVKITITITWLSELEKQVDEHVTAARRDGRFRYEFQP
                     SQPAPGAQYVITVTASLRTGESQTASITVSEH"
BASE COUNT         1089 a         1604 c         1657 g         1064 t
ORIGIN      
        1 gtgatagagt caatccggat aatgcgattg ttgaacacgt ccgcgacgta gatccgcccc
       61 gtaccgtcga caaaaacacc tcgaggacgg ttgaactgat ccgttccgct gccgagactg
      121 ccgaacgaag tccaggccac ccccgccatg tcgttcattc gaacgatgcg gtgattagat
      181 cgctcgccga cgtagatgcg cccggaacta tcgacgtgaa tccccgcggg ctggttaaac
      241 tggttcgttc cgccgccgag tgttccaaat gcgacccacc cggacccggt catgtcattc
      301 acccggacga tccgatggtt gtcgcgatcc gcgatgtaga tccgaggacc tgtgggaatg
      361 ggactgatca gcccgacgcc attacacccg acggcggcga ggccgagcag gataacgagg
      421 agtctgcgca ctgctaccat tcggatggga acgttgttcg tgggctcgat actattccct
      481 tttcactaaa gtcgagtgta ccttacgtgt acttccgata ggactgtctt ctccgctcgc
      541 tgaaccacgg aacagatggc tcagactgtt ggtgactcgt tggaagccgg ccgcaaggcc
      601 attaagggac acgcttggcg tgaggcgttc gaacttttgg gcgctgccga tccctcaaca
      661 ctcacggccc aggactatga gcatctcgcc gaggccgcgt ggtggaccgg acggctggat
      721 gcgtgtatcg acgcgcgtga acgcgcatac aacatctacc ttcaacaagg gaatcagagg
      781 cgggcagggt cgatggctct tgagctagct cacgatcacg agttcaagcg ggcacccccg
      841 atcgcggcag gatggcagag caagggagaa aggctgctcg agcaggagcc agaaggtccc
      901 gagcacggcg ttcttagccg acggcgcacg aatgcggcga tgggcaaggg agattttgaa
      961 tctgccctgg cccacgcgaa gcgaacggtc gatattggca tgaagttcgg ggaccgcggc
     1021 ctccagggga tgggcctaca tgatcaaggg cgcgtcctca tcgctatggg gagagccgac
     1081 gaggggatgg cgttgataga tgaagcaact gtcgccgccg tcagcggcga acttcagccc
     1141 ctgccaacag cggtcatcta ttgcaacacc attggcacgt gccgagacat ggtggattac
     1201 cagcgggccg gccagtggtc cgaagcagcc aagcgttggt gtgaccgcca gcatatcacc
     1261 ggttttccgg gaatgtgccg ggtttacagg gccgagatca tgcgactccg aggggcgtgg
     1321 accgaagccg agcaggaggc caagcgcgct tgtgaagagc tgaaagattt ctgcctcgac
     1381 tatgccgcgg gcgctttcta tgaagtggga gagatccggc tccgggtcgg tgattttcag
     1441 gccgccgaag cggcgttcaa ccatgcgcat gagataggta aggatcccca gccgggatta
     1501 gccctgcttc gactggccga ggggaagatc gcgggggctc aggcctccat ccgtagggct
     1561 ctggaagaaa cccacgatcg attggctcga gtccggctcc tcccggcgga ggcagaggtc
     1621 gctctcgctg cgggtgagat tgagagtgct cggagtgccg tggaggagtt ggaagccatt
     1681 gcaaaggatt acagcacacc cgcactattg gcgtacgcct cctactgccg gggcgctctt
     1741 caactcgcta caggcaacac agttcaggcc cgcgacagtc tacgtcagag tattcgtcta
     1801 tggcaagaca tcgaatcacc ctatgaaatg gcgcgcgccc gcctgctgct gagtacaacc
     1861 taccgtgcta tgggcgatat ggaagatgcc ggccttgagc ttcacgctgc gcggtccgtg
     1921 ttcgagcgtc taggggcgat tccggatctg cgacgggcgg atagtctact taaagaagaa
     1981 ggagccgccg gccgttccgt gcctaccgga aggcaggcga gccggacatt catgtttacc
     2041 gacgtcgtca gatcaactcc gttggttgaa gcgatcggcg atgcggcgtg ggcggatgtt
     2101 gtgcggtggc acgatcagac gctacgttca ctctttgcca cgcacgaggg ggaagaagct
     2161 gatcacgcgg gggatggttt cttggtcgcc ttcaacgact caacgacagc agtagagtgc
     2221 gcggtggcaa tccaacgagc gctcgccgaa catcgccata ctcacgggtt cgcaccgcag
     2281 gtacggattg ggattcacac aagtcaagca gtccaggagg gcggaaagta caagggaaag
     2341 ggcgtgcacg aagcggcacg cattggcgca cttgctgagg gaggcgagat cgtagtgagc
     2401 ggcgataccc tcgccggatc tccgccgcgg ttcccagcca tcaacccccg cacagtcacc
     2461 cttaaggggc tcagccagtc agtcacggtc tgcagcctgg actggcgata gcgcccaagc
     2521 agagtaggta gatttcttca gaagatctcg ctaggcgccg agcgctttgg cagccttttc
     2581 gaagtcggcc ggcgcgaccc acaacaacat gccgtaacgg cctgatcctg ccccgaccgc
     2641 cgtcaccgcg ctgatattga ccttggcgtc tgcgagcttc ttcgcgtgtt cggccgctgc
     2701 gccaacacgg tcgtcgccgg tgatcaggaa tgccgacttc ggtcccgtca gggtgacgcg
     2761 ggctgattgc gctgcctgct tgaacttccc ggcgttctcg ggaacgaaat cgacctgagc
     2821 ttgtcctccg ctagtcggga acgaatggaa cgccaagagg agcacgccgg catctttgag
     2881 cgcagaaagc actcttatgc cctcgcccgg tttatctggc acggttgcgt agaagtactc
     2941 gactctccga accttgtccg ccattaccct cacctcccga tggttcctac gctttatgcg
     3001 atgcgcgctg tataatcctg caagtttatt accaaaaacc tctcaattcc tgccgactta
     3061 tcgtcgattg ccccgggcaa acccaagata tactcacgag gcctgcttct tgcttgtgct
     3121 tagcaagagg gcgaacaccg tcggtgttcg atcttccgcg actcggggtt cacaatgctc
     3181 tctacagcga ggcgggcgat tggggcggct ctccttattc tcgcattagt tctcgccttt
     3241 gagctccctg tttccgcagc gaaaaagcaa cagcaaccaa ggctgacgcg gcagcaactg
     3301 atcgccttgg tcaacaagta tgcggccaag tacggaatcc cccaggcgat tgcccataat
     3361 ctggtcgagg tggaatcggg ctggcggcag aacgcggtga gttatcgcgg tgcgcgcggg
     3421 atcatgcaat tgatgcccat gacggctaga gaccttgggg tcaaccggcg cgatccgaag
     3481 cagaatatcg aaggcggaat gcggtacctg cgtcagctct acaatagatt ccgctcatgg
     3541 gacctggccc tcgcagccta ccactctggc ccgcgaaacg tgatcaggca caaaggcatt
     3601 ccgccagtat ctcgatccta cgtcaggcgg attctcagga actccggatc tgttgcatat
     3661 gcatcgcccg cgcgccgcgc cgcaaaggcc gcgccggcta cggtccccac tccgggattt
     3721 gaaaagcgac agtctgtgac cgtttcggga cagacaacca cgacggtccg ggaaagtcta
     3781 cgcgatggtc aggtggtctg gcgggcggaa gatatcgtca ccgtacaggg caacgtgcgg
     3841 attaacatga cacgtgagta ccggctggtc gacgggacgc tgacgctcgt gaaagagcag
     3901 accctcgtat atacaatcac ggacgagagc atcaccgagt ccgtgccgaa cgaagagaaa
     3961 tagttagtgc tctgataccg tgatcgaagc ggtttgagac tcgcctgtcc gaagtgacgc
     4021 agtgaccgta atcacgtatt gcgctcctgg tgccggctgc gaaggctgaa actcgtatct
     4081 aaaccttccg tcacgcctcg ccgccgtcac atgctcatcc acctgtttct ccagctcact
     4141 cagccaggta atggtaatcg tgattttcac tcccggggtc gtcgtgcctt cgatcgtgaa
     4201 gggcgtcgta agagcgaccc cattggccgg ggcagtgacg tttaggggta ggggagagcg
     4261 cacaggttgg tccggcagca ccgggttctg ttgcggcgga atgactccag gagagcccgg
     4321 tttgccggca ccggatgcgt actccgtagg ctccgttccc tcgatgaaga tttcacgccg
     4381 gggatctgtg cagtcaccgg ccgccagcag cccggatgtc ccgcagacca tacgctccga
     4441 gacgccggcc ggcatcgtcc agtcgtccgg cgggaggttc tgtgtggccg ggaccatgaa
     4501 tgacgcccaa atccttgtcg gaacggtcgc tccgacaacc tttttcatcg gtgagttatc
     4561 gtcgttgccg acccatacgg tagtcacgag cgaaggagtg tacccgacga accaggcatt
     4621 ccgatagtcg tcggttgtcc cggtcttgcc tgccacaggc cggccgagcg ccgcgccccg
     4681 cccggttccg cgggcgatca cgcctttcag cagatcggtc atgacgtagg ccacgtcggc
     4741 gctcagcgcc agttcctggc gtggctcatg ctcctccaga acctggccgc cgcgagcctg
     4801 gatcttctta ataggaatcg gctcggtcct gaccccgagc cctgccagtg tgccgtaggc
     4861 agacgtcatc tcgagcggcg tgacgtcatt gctgccgagc gccagcgaca ggttcggctg
     4921 gagcgggctc ttgatcccca tccgcctggc gtattcgatg acacgcacgg ccccgagctc
     4981 ggcaatcgtt ttgaccgccg ggatattgat cgagttctcc aacgcgtacc gcatggtgac
     5041 cgggccgcgg aacttttcgt cgtaattctt cggctgccag tgctgaaact caggcttcgc
     5101 atctgtgacg aagtactcga ccggaacatc ctcgatgatc ttcgtgggcg gcatgccgtt
     5161 ggcaagcgcg gtgacataaa tgaacggttt gaacgcggag cccggctgac ggcgcgcctg
     5221 ccaggcccgg ttgaattgac tctggttgaa atcgtagccg ccgaccatgg cgcggacgta
     5281 accggtgcga ggctcgatcg ccaccagggc accctgggtg acgttcagct tctggcgctg
     5341 agcttcgtca atgccctccc gcacggccag ttcagcggcg atctgcatgt ccagatcaag
     5401 cgtcgtaaat acct
//
LOCUS       DBNGSB010000060         6472 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2338825, whole genome shotgun sequence.
ACCESSION   DBNGSB010000060 DBNGSB010000000
VERSION     DBNGSB010000060.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 6472)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6472)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6472
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2338825"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="AC159B_01685"
     CDS             <1..266
                     /locus_tag="AC159B_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HIR3376499.1"
                     /translation="PVAQSVEAVKQTLERTPPELAADIVDRGIILAGGGSLLRGVDRL
                     LAEETGMPVTLTDDPLSAVALGTGRALEEIEMLKRVLITSRKL"
     gene            276..1100
                     /gene="mreC"
                     /locus_tag="AC159B_01690"
     CDS             276..1100
                     /gene="mreC"
                     /locus_tag="AC159B_01690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HIR3376500.1"
                     /translation="MFDVIIARRRIAVLVGLILFTLVLLTSQVRSADRRQVGPLGTVV
                     LTLLLPIQAGMAHVADAGTRWWRLYTEIGRLRQENARLRSEVERLSQDLTLIREQAIS
                     ARRMERLLEFRPQPGYRAVAARVIARDPERWFSTLLVDRGSRDGVRRNAAVATADGVV
                     GRVIEVTPTAARVLLIGDSRSAMSVLVQRSREAGIVEGRGGEMLHLKYLSRTGDVAAG
                     DLVVTSGLGGVFPRGLVVGQIVNINREPGALQLEAEVRSAASVARLEEVVILVAKL"
     gene            1103..1615
                     /gene="mreD"
                     /locus_tag="AC159B_01695"
     CDS             1103..1615
                     /gene="mreD"
                     /locus_tag="AC159B_01695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HIR3376501.1"
                     /translation="MLGSDRINRALMYGGVLIVAALVQTAWLARLPVVGAVIDPVLVL
                     AVATGILRGAESGAVVGLAGGLLQDLLSGGPLGMHGLSKLVAGFGSGLFERSIYIENP
                     FLPAVAAFAGTLLGEALLNVIGVVTGLGSVSLTEAVPRVVIQAGLNSVIAPPLFRGIR
                     SLDVRFERQH"
     gene            1669..3480
                     /gene="mrdA"
                     /locus_tag="AC159B_01700"
     CDS             1669..3480
                     /gene="mrdA"
                     /locus_tag="AC159B_01700"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HIR3376502.1"
                     /translation="MDFQAPRQRLFALSLTIAIAIGILVGRLWQIQVLQGTAYLRQSE
                     ENRIRDYTLTAPRGIVYDRKGRPLVTNRPSFTVAILPLELRRPAEVMAALAPMLGMTP
                     GEIRARIESSRSRLFEPVRVKRDVGTRVVAMIEENRMDLPGVVILAEPVRHYLHGKMT
                     AHVMGYIGEIGEDELKARKSEQYKLGDLIGKTGVERKYEAALRGSDGRLRMEVDALGR
                     PLRVLGRVAAVPGRSIVLSLDLDVQRAAFEALLNSGLPSGAVVAMDPRNGELLAMVST
                     PSYDPNLFATGISPRAWQGLVRHPLRPLLNRASGTAFEPGSVFKLVTATAALERGIVT
                     RHSTFHAPGYFRLGRWVFHDLRAWGTVNFMTGIANSINVVFYTLGHRLGGELLALYAK
                     MLGLGEITNVDLPAEVSGTIPSPSTKQQLVGEPWYPGDNVNMSIGQGAVTATPLQVAR
                     MVSVIANRGTLYQPRVLLMMHDKDGKPRRTEPVLQGKVKLKPSTFDVLHEGMQAAVDR
                     GTGRAAAISGLAIAGKTGSAENPRGKPHAWFAGFAPVRAPTVVVVAFVEHGFRGGIAA
                     APIVRKVFLAALPQAPDQRAAPHQTAHRTVHVLEPRR"
     gene            3477..4571
                     /gene="rodA"
                     /locus_tag="AC159B_01705"
     CDS             3477..4571
                     /gene="rodA"
                     /locus_tag="AC159B_01705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HIR3376503.1"
                     /translation="MSARLLRFVDWILLGTTLALMVFGGVMIYSATQSTGDPMGFVKV
                     RVLHALFGLTLLAVAAAVDYQAVARAWRPVLGGSMFLLVVVMLVGVTTMGSQRWIPLG
                     PLGGFQPSELAKLALIIALARNLDAHPDMELWRTVIPVLVIAAVPVGLIVEQPDLGTA
                     MVLVAITAAMLFAAGAPRRIMLALGGAALAGVPVLWQMLHDYQRRRLIVFIDPGVDPQ
                     GAGYALIQSKIAMGSGQVFGKGLLHGTQNLLNFIPEQHTDFIFTVVGEELGFLGAMTV
                     LALFAIWLWRGMTIVYLAKDRLGMLMAVGIVCMIAFHVIVNIGMTVGLMPITGIPLPF
                     ISHGGSALVVMLMATGVLLGIGMRRKKILF"
     gene            4594..6246
                     /locus_tag="AC159B_01710"
     CDS             4594..6246
                     /locus_tag="AC159B_01710"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HIR3376504.1"
                     /translation="MSKEIIANIEPFETRVAVLEDGQLVNFFVERGEPLAGNIYKGRV
                     ANVLPGMEAAFIDIGLERNAFLHVGDVRAQRLAGEEVDDSFGRGAIAERLRVGQEILV
                     QVTKEPMGTKGARVTTYVALPAYYLVLMPTVNYVGVSRRIERDQERRRLRQLADKLRP
                     KGMGVIVRTAAEGASEKDLADDIQFLLQVWSRVEERSKTGRAPTLVYQDLRLIRRVVR
                     DLFTDDVARFLIDSPEEFQRVQDLVASFAPKLKARLSLHSASEPVFEALGVERELEKA
                     LRRKVWLRSGGYIVIDRTEALTVIDVNTGKYVGKTDLATTIFRTNLEAVGEIVRQITL
                     RDIGGIILVDFIDMENESHRKRVMQAVNEAVRKDRSKIHIIDLTGLGLVEITRKRVYQ
                     DLEEIMRIPCPYCEGRGRILSPETMSMRTRREVRRLMRTTSATAALVEVHPDVYKELF
                     RDGPSWVRQLEEQTGRRLTIRGRDGMHLERMRVLEGERTEDLDREETARDRRGQVYWL
                     DREGEDTLVFSERDVEQELALAAAGNSRNGLMRRLLKLLGRS"
BASE COUNT         1144 a         1863 c         2234 g         1231 t
ORIGIN      
        1 agcctgtcgc ccaaagtgtc gaggccgtca aacagacgtt ggagcgaaca ccgccggagc
       61 tggccgcaga tattgtggac cgcggcatta ttctggcagg aggcggatcg ctgcttcgcg
      121 gcgtggacag gctgctggcc gaagagaccg ggatgcctgt gacgctcacg gacgatccgc
      181 tctcagcggt ggcgctcgga accggtcggg cgctggagga aatcgaaatg ttgaagcgcg
      241 tgttgattac gtcgcgaaag ctctagggag ccgaagtgtt cgacgtcatc atcgcacggc
      301 gacggatcgc ggtccttgtt ggcctcatcc tgtttacgct cgtgctgctg acgagccagg
      361 tacgcagcgc cgaccggcgg caggtcgggc ctctcggcac cgtggtgctg acgttgctgc
      421 tgcccattca ggccgggatg gcgcatgtcg ccgacgcggg aacacgatgg tggcggctgt
      481 atacggagat cggacggctg cggcaggaga atgcgcggct gcggtccgag gtcgaacgct
      541 tgtcacaaga tctcaccctc attagagaac aggcgatctc agcgcgtcgc atggagcggc
      601 tgctcgagtt ccggcctcag cccggctacc gggcagtcgc cgcacgtgtg atcgcgcgcg
      661 atccagaacg gtggttctcc acgcttctgg tcgaccgtgg gagccgcgat ggtgtccggc
      721 gcaacgcagc ggtcgcaacc gcagatgggg tcgtcggcag ggtgatcgag gtcacgccga
      781 ctgccgcacg ggtgctcctg atcggagact cgcgcagcgc catgagtgtg ctggtgcagc
      841 ggtcccgcga agccgggatc gttgagggtc gtggcgggga gatgttgcat ctgaagtacc
      901 tgtcgcggac gggtgacgtg gctgcgggtg atctcgtggt cacttcaggg ctgggtggcg
      961 tcttcccacg ggggctcgtg gtggggcaga tcgtgaacat caatcgggag ccgggggcat
     1021 tgcaacttga agcggaagta agatcggcgg catcggtcgc gcggcttgag gaagtcgtga
     1081 tcttggtggc aaagctttaa cgatgcttgg atcagatcgg ataaataggg ccctgatgta
     1141 cggcggcgtg ctgatcgtgg cggccctggt gcagaccgca tggctggcca ggctgccggt
     1201 cgtcggcgcg gtgatcgatc ccgtattggt gctggctgtg gcgacgggca ttctgcgggg
     1261 agcagagtcc ggcgccgtcg tcgggctggc aggggggttg ttgcaagatt tgctgagcgg
     1321 aggcccgctg gggatgcatg ggttgagcaa gctggtcgcc gggttcggtt ccggtctctt
     1381 tgagcggagt atttatatcg agaacccctt tctgcccgcc gtggctgcct tcgcagggac
     1441 tctcctcggt gaggcgctgt tgaatgtcat cggcgtggtg accggtttgg gatcggtgtc
     1501 attgactgaa gccgtccccc gcgtggtcat ccaggccggg ctcaacagcg tcatcgcccc
     1561 gccgctgttc cgcggcatcc gctccctcga cgtgaggttc gagagacaac actgaccgcg
     1621 caagcgcgga aacgcgcaag cgcgtaatca gcagcgtaaa ggagttgcat ggattttcaa
     1681 gcgccgcgac agcgcctgtt cgcgctgtcc ttaaccatcg cgattgcaat cggaatcctg
     1741 gtgggccggc tctggcaaat ccaggtcctc caggggacgg catacctgcg ccagtcagaa
     1801 gaaaaccgga ttcgcgatta cacgctcacg gcgccccgcg ggatcgtcta cgatcgaaaa
     1861 gggcgtcctc tcgtcacgaa ccggccttcg ttcacggtcg caattctgcc tctcgaactg
     1921 agaaggcctg ccgaagtcat ggcagcgctg gcgccgatgc tcggcatgac ccccggggag
     1981 atccgcgcgc gaatcgaaag cagccgctct cgcctgtttg agcccgtgcg cgtcaagcgg
     2041 gacgtgggca cgcgggtcgt ggcgatgatc gaggaaaatc gaatggatct tccgggcgtc
     2101 gtcatccttg cggagccggt acgtcactac ctccatggaa agatgaccgc gcacgtcatg
     2161 gggtacatcg gcgagatcgg tgaggacgaa ctcaaagcga gaaaaagcga gcagtacaaa
     2221 ctcggggatc tcattgggaa gaccggcgtg gagcgcaagt atgaggcggc gctgcgagga
     2281 agcgacggcc ggctcaggat ggaggtcgat gcgctcggca ggccattgcg ggtgttgggc
     2341 cgcgtggccg cggttccggg acgatcgatt gttctgagcc tcgatctcga cgtccagcgg
     2401 gccgcctttg aggccctgct taatagcggc cttccgtcag gtgcggtcgt ggcgatggat
     2461 ccccgcaacg gggagctgct cgccatggtg agcacgccat cgtatgaccc caatctgttc
     2521 gcgaccggta tctcgccaag agcctggcag ggtctcgtcc gccacccgct ccgcccgctt
     2581 ctcaaccgag cgtcggggac ggcgtttgag cctgggtcgg tcttcaagct ggtgacggcg
     2641 acggccgccc tggagcgggg catcgtcacc aggcacagca cgttccatgc acctggatac
     2701 ttccgcctcg gccggtgggt gtttcacgac ctgcgagcct gggggacagt gaacttcatg
     2761 accggcatcg cgaattcgat caacgtggtc ttctatacgc ttggtcaccg gctcggcgga
     2821 gagctgctcg cgctctacgc gaagatgctg ggtctcgggg aaatcaccaa tgtcgatctg
     2881 cccgccgagg tttccggaac gatcccgagc ccgtcgacca agcagcagct ggttggggag
     2941 ccctggtatc ccggggacaa cgtgaacatg tcaatcggtc agggggccgt gaccgcaaca
     3001 ccgctgcagg tggcacgaat ggtgtcggtc atcgccaacc gaggaacgct gtatcaacct
     3061 cgcgtgctgc tgatgatgca cgacaaagac ggcaaaccgc gccgcactga acccgtcctg
     3121 cagggaaagg tgaagctgaa gccctcaacg ttcgacgtcc tgcacgaagg catgcaggcg
     3181 gcggtggacc gaggcaccgg ccgcgctgcg gccatcagcg gcctcgcgat tgcgggcaag
     3241 acagggagtg ctgagaaccc ccgcggcaag ccgcatgcct ggttcgcagg cttcgcgccc
     3301 gttcgcgcgc cgacggtcgt ggtcgtagcg ttcgtcgagc acgggtttcg tggaggcatc
     3361 gcggccgcac cgatcgtgcg taaagtgttt ctggcagcgc tgccgcaggc accggatcaa
     3421 cgtgcggccc cgcaccagac ggcgcaccgt accgtgcatg tcttggagcc acgccgatga
     3481 gcgcccggct gcttcgcttc gtcgattgga ttctgctcgg gacgacactc gccctgatgg
     3541 tattcggggg cgtgatgatc tacagcgcca cgcagagcac tggggatccg atgggcttcg
     3601 tgaaggtccg tgtccttcac gcgttgttcg gtctgacact cctggccgtg gcggccgcag
     3661 tcgactacca ggcggtcgcc cgggcctggc gcccagtgct gggcggctct atgttcctgc
     3721 tcgtcgtcgt catgctggtt ggtgtcacca cgatgggatc gcagcgctgg atcccgctcg
     3781 gaccgttggg aggctttcag ccatccgagc tggcgaaatt ggcgctgatc atcgccctcg
     3841 ccaggaatct cgacgcgcat cccgatatgg agttgtggcg cactgtgatt cccgtgttgg
     3901 ttattgccgc tgtgcctgta ggcttgatcg tcgagcaacc ggacctggga accgcgatgg
     3961 tgctggtggc gattaccgcg gcgatgcttt tcgccgccgg ggcgcccaga cggatcatgc
     4021 ttgctctcgg cggcgcagcc cttgccggcg ttcctgtgct ttggcagatg cttcacgatt
     4081 accagcgccg ccgcctgatt gtcttcatcg atccgggtgt ggacccgcag ggggcggggt
     4141 acgcgctgat ccagtccaag atcgcgatgg gctcgggaca agtattcggg aaagggctcc
     4201 tgcatggaac ccagaacctg ctcaacttca ttccggagca acatacggac ttcatcttca
     4261 ccgtcgtggg agaggaactg ggattcctgg gcgcgatgac tgtcctggcg ctgtttgcga
     4321 tctggctgtg gcggggcatg accattgtct atctggcgaa ggacaggctc ggcatgctca
     4381 tggcggtcgg catcgtgtgc atgattgctt ttcacgtcat cgtgaacatc gggatgacgg
     4441 tcgggctgat gccgatcacc ggcattcctc tgccgttcat cagccacggc gggagcgcgc
     4501 tcgtcgtcat gctgatggcg acgggcgtat tactgggcat tgggatgaga cgaaaaaaga
     4561 ttctattctg agcactgtga tgggagtcat cgaatgagca aggagatcat cgcaaacatc
     4621 gagccgttcg aaacgcgtgt cgcggttctg gaggacggcc agcttgtaaa tttcttcgtc
     4681 gaacgggggg aaccgctcgc cggcaacatc tacaaagggc gcgtagcgaa cgtcctgccg
     4741 ggcatggagg cggcgttcat cgacatcgga ctggagcgta acgcgttcct gcacgttggc
     4801 gatgtccggg cgcagcgcct ggcgggtgaa gaggtcgacg actcgtttgg ccgcggcgcg
     4861 atcgccgagc gactgcgggt gggtcaggag atcctggtcc aggtgaccaa ggagccgatg
     4921 ggcacgaagg gcgcgcgcgt gacgacgtac gtggcgctgc cggcgtacta tctcgtgctc
     4981 atgccgacgg tgaactacgt cggggtcagc cggcggatcg aacgtgacca ggaacgcagg
     5041 cgcctgcgtc agctcgccga caagctacgc ccgaagggca tgggcgtcat cgtccgcact
     5101 gccgcggaag gcgcgagtga gaaggacctc gcggacgata tccagtttct gctgcaggtc
     5161 tggtcccggg ttgaggaacg atcgaagacc ggccgggcgc cgacgctcgt gtatcaggat
     5221 ttacgtctca tccgtcgggt tgtgcgggac ttgtttaccg acgacgtggc ccgattcctc
     5281 atcgactcgc ccgaggagtt tcaacgcgtc caggatctcg tggcctcctt cgcgcccaag
     5341 ttgaaggcgc gcctcagcct gcatagcgcc agcgagcccg tgtttgaagc gctcggcgtg
     5401 gagcgcgagc ttgagaaggc cctgcgccgc aaagtgtggc tgcgctccgg cggctacatc
     5461 gtcattgacc ggacggaagc gctgaccgtg atcgacgtca acaccgggaa gtatgtgggg
     5521 aagacagatc tcgccacgac gatctttcgc accaacctcg aggccgtcgg ggagatcgtc
     5581 aggcagatca cgttgcggga catcggaggc atcatcctcg tcgacttcat tgatatggag
     5641 aacgagtccc accgtaagcg cgtcatgcag gcggtcaatg aggccgtgcg taaagaccgt
     5701 tcgaagatcc acatcatcga cctcaccggg ctcgggttgg tcgaaattac gcgcaagcga
     5761 gtctatcagg atctcgaaga gatcatgcgg atcccctgtc cctattgcga agggcgcggg
     5821 cgtatcctgt cgccggagac gatgagcatg agaactcgcc gggaggtccg ccggctcatg
     5881 cggacgacct ctgctacggc agcgctggtc gaggtacatc cggacgtgta caaggaactg
     5941 ttccgcgacg ggccatcgtg ggtgcgccag cttgaggagc agacgggacg gcggctgacg
     6001 atccgcggcc gggatgggat gcacttggaa cggatgcgcg tgctcgaagg cgagcggact
     6061 gaggatctgg accgggaaga aacggcgcgt gaccggcgtg ggcaagtgta ctggttggat
     6121 cgtgaaggag aggacacgct tgtcttcagt gagcgggatg tcgagcagga actggcgctt
     6181 gcggctgcgg gaaactctcg caacggattg atgaggcggc tgctcaagct tctcgggcgt
     6241 tcatagttcg cgaggtcctt gtgcacggcg gcgggacggg gaccttggac cggggacccg
     6301 gcaatcggct atggagtgac ttagttacgg gtcccctttc tccaagatcc ccatcccgcc
     6361 gccaagaacc ccgtgcacga gctgttgagg cgtctgtgtg catgtgatac gatacgcgag
     6421 gcccaaagcg cagcgcgctg tgtgtggagg acgggatgta cgcagttatc ga
//
LOCUS       DBNGSB010000061         7177 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_806441, whole genome shotgun sequence.
ACCESSION   DBNGSB010000061 DBNGSB010000000
VERSION     DBNGSB010000061.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 7177)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7177)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7177
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_806441"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3296)
                     /gene="rpoB"
                     /locus_tag="AC159B_01715"
     CDS             complement(<1..3296)
                     /gene="rpoB"
                     /locus_tag="AC159B_01715"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012268991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIR3376505.1"
                     /translation="MVGHPVTRGHRTRESFAKIPEILDVPDLIEVQRRSYEWFLREGI
                     REVFEEVSPIQDFTGNLELHFAVEGRGKRPDPPSLFNDDQVMDFGGYRLERLKYSEEE
                     CRDRDYNYSAALKVKVRLLIKETGEIKEQEVFMGDLPLMTRRGTFIINGAERVVVSQL
                     VRSPGVYYNYGEDASGRPMPTATVIPHRGAWLELELDNNGVISTRIDRTRKIPVTVLL
                     RALGYDSDEKILKLYEHDKTIQATLAKDPVKERAGEKAYQEALIEIYRRLRPGDPPTV
                     ESARQLLQTLFFDPRRYDLGKVGRYKVNRKLDLEAPLGFRTLVQTTGGELADVRVSRD
                     RKEFTLELRDGREFTGQTAKVTVRIDKEPADVRKLEKGSRVVLGLVSINKEVLYVISE
                     EGVRHPAAVRDDIAEVVRYLIRLHNEEEGTTVDDIDHLGNRRVRSVGELLQNQFRIGM
                     LRMERVIRERMTIQETQQITPQVLINIRPVVAAIKEFFGSSQLSQFMDQTNPLAELTH
                     KRRLSALGPGGLSRERAGFEVRDVHTSHYGRMCPIETPEGPNIGLISSLATYARVNDL
                     GFIETPYRVVRDGNVTRRSEFLTADQEDKYIVAQANAKIDDRGHLTESRVSARHGSLE
                     IVLVSPDTVDFMDVSPKQTVSVATALIPFLEHDDANRALMGSNMQRQAVPLLDSEAPL
                     VGTGMEYRAAADSGAVVLARRPGTVESVSGDEIVIKSDRGRDTYKLIKFQRSNQGTCM
                     NQTPAIQTGDEVKEGEVIADGPCTDQGELALGRNVLVAFMPWEGYNSEDAIVISERLV
                     KEDLFTSIHIEEYEVEARDTKLGPEEITRDIPNVGEEALRDLDERGIVRIGAEVRSGD
                     ILVGKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGEKGKVVSVKVFSRDKG
                     DELPPGVNTLVRVYVAQKRKITVGDKMAGRHGNKGVISVILPEEDMPSLPDGTPVDIV
                     LNPLGVPSRMNVGQVLETHLGWAAAQLNIWAECPVFDGPKEHEIRKHLIEAGLPPEGK
                     VELHDGRTGEEFDQKVTVGWIYMMKLLHLVEDKIHARSTGPYSLITQQPLGGKAQFGG
                     QRFGEMEVWAL"
     gene            complement(3667..4047)
                     /gene="rplL"
                     /locus_tag="AC159B_01720"
     CDS             complement(3667..4047)
                     /gene="rplL"
                     /locus_tag="AC159B_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HIR3376506.1"
                     /translation="MGKDEIVDAIGNLTVLELADLVKALEEKFGVTAAAPMAVAMPGG
                     GGAGAAAQAVEEQTEFDVVLKTIGDKKIQVIKVVRELTGLGLKEAKDLVDGAPKPVKE
                     KVGKAEADQMKTKLEAEGATIEIK"
     gene            complement(4098..4715)
                     /gene="rplJ"
                     /locus_tag="AC159B_01725"
     CDS             complement(4098..4715)
                     /gene="rplJ"
                     /locus_tag="AC159B_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HIR3376507.1"
                     /translation="MAKRKTQPKKVEPDRGARPRKVGQVESIRERLDSAAAAILTDFR
                     GLNVGEISQLRSKLRDAGAEYKVVKNTLLERAAESLGIRGLEPYLQGPTAVAFSNRDP
                     IAPARILAEYMRQMKKLEVKAGLVEGRVLTADQVKVLADLPSKNQLLAMALRNMKSPL
                     VGVAGVLVALQRNLVYALDQIRQKQAPQYETVQTVQAVQTVQTVS"
     gene            complement(5001..5720)
                     /gene="rplA"
                     /locus_tag="AC159B_01730"
     CDS             complement(5001..5720)
                     /gene="rplA"
                     /locus_tag="AC159B_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004094873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HIR3376508.1"
                     /translation="MKKRHGKRYRDAAKLAGEDQLHDPTGAIKILKQAPKAKFDETVE
                     VAIRLGIDPKQADQQVRGTVALPAGTGKKVRLLVFAKGEKAKEAESAGADMVGADEFI
                     EKIQGGWTDFDVAVATPDMMSTVGKLGRILGPRGLMPNPKSGTVTFDLNKAVKEFKAG
                     KIEYRTEKAGVIHAPIGKASFSEDQLLENFTSLIEAVSRAKPAASKGPFLMSITLSTT
                     HGPGIKIDPAKAQALLKTATT"
     gene            complement(5722..6147)
                     /gene="rplK"
                     /locus_tag="AC159B_01735"
     CDS             complement(5722..6147)
                     /gene="rplK"
                     /locus_tag="AC159B_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007070817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HIR3376509.1"
                     /translation="MAKKVIAIVKLQIPAGKATPAPPVGPALGQHGVNIMEFCKSYNE
                     RTASQAGTIVPVEITVFADRTFSFITKTPPASVLLRQAAGLEKGSGQPNKNKVGKVTR
                     AQVRGIAEKKLKDLNANDLDAAVRMIEGTARSMGIEVTS"
     gene            complement(6160..6918)
                     /gene="nusG"
                     /locus_tag="AC159B_01740"
     CDS             complement(6160..6918)
                     /gene="nusG"
                     /locus_tag="AC159B_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HIR3376510.1"
                     /translation="MEDKRPVIEDKIKELFGDEPVAEPEPAPAPVSDPEPAVVEEPAA
                     RAELEVQVEPNGEAAVEAPEAEAPTEPEAPRDPRLRWFVIHTYSGYENKVKTNLERRT
                     KTMHGKGGEKIAQVLVPTEKEKEIKGGKRREVDRKIYPGYVLVEMVMDDDSWYVVRNT
                     PGVTGFVGSGARPVALEDQEVKRLLKQIHDETPKYRINFQKGSMVRITTGPFMDFTGQ
                     VDDVMVEKEKVRVLVSIFGRSTPVELNFGDVEKV"
     gene            complement(6931..>7177)
                     /gene="secE"
                     /locus_tag="AC159B_01745"
     CDS             complement(6931..>7177)
                     /gene="secE"
                     /locus_tag="AC159B_01745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HIR3376511.1"
                     /translation="RQAVRPAAKPAPVRTGLPIIERIRRYLREVRTELGRVDWPSRAE
                     LTAMTIVVVVVLLLMSLYLGAWDLLFTWLFQRGLVRQ"
BASE COUNT         1191 a         2323 c         2087 g         1576 t
ORIGIN      
        1 agcgcccaga cctccatctc cccgaagcgc tgcccgccga actgtgcctt gccgcccagc
       61 ggctgctggg tgatgaggct ataggggccg gtgctgcggg cgtggatctt gtcctcgacc
      121 aggtgcagca gcttcatcat gtagatccag cccactgtga ccttctggtc gaattcctcg
      181 ccggtccggc cgtcgtgcag ttccactttg ccttcggggg gcaggccggc ttctatgaga
      241 tgctttctga tctcgtgctc cttggggcca tcgaacacgg ggcactcggc ccagatgttc
      301 aactgcgccg ctgcccagcc caggtgcgtc tccagcacct gaccgacgtt catgcggctt
      361 ggaaccccca gcgggttcag cacgatgtcg accggcgtgc cgtcgggcag cgacggcatg
      421 tcttcctcgg ggagaatgac ggagatcacg cccttgttgc cgtggcgtcc cgccatcttg
      481 tcgcctacag tgatcttccg cttctgcgcg acgtagacgc gcaccagcgt gttgacgccc
      541 ggcggcagct catcgccctt gtcccggctg aagaccttga ccgacacgac cttacccttc
      601 tcgccgtgcg gcaccttgag cgacgtgtcg cgcacttcgc gggctttctc accgaagatt
      661 gcgcgcagga gacgctcctc ggcggtgagc tcggtctcgc ccttcggcgt gaccttgccg
      721 acgagaatgt cccccgagcg gacctctgcg ccgatgcgaa cgatgccgcg ctcgtcaaga
      781 tcgcgcagcg cctcttcacc gacgttgggg atgtcgcgcg tgatctcttc cggcccgagt
      841 ttcgtgtcac gggcctccac ttcgtactcc tcgatgtgaa tcgacgtgaa caaatcttcc
      901 ttgacgaggc gctcgctgat gacgatagcg tcctcggagt tgtagccttc ccagggcatg
      961 aacgcgacga gcacgttgcg gcccagcgcc agttcaccct gatcggtgca cgggccgtcg
     1021 gcgatgacct cgccttcctt gacctcgtcg ccggtctgaa tcgcgggcgt ctggttcatg
     1081 cacgtccctt gattgctgcg ctggaacttg atcagcttgt atgtgtcccg gccccgatcg
     1141 gacttgatca cgatttcatc gccggacacc gactccaccg ttccgggacg gcgcgcgagg
     1201 acgacagccc ccgagtcggc cgcggcccgg tattccatgc ccgtgccgac cagcggcgcc
     1261 tcgctgtcga gcagcggcac cgcctggcgc tgcatgttgg aacccatcaa cgcgcggttg
     1321 gcatcgtcgt gctcgaggaa cgggatcagc gcagtggcca cggagaccgt ctgtttgggc
     1381 gacacgtcca tgaaatcgac cgtgtccggg gacacgagca cgatctccaa actcccgtgc
     1441 cgcgccgaaa cgcgagattc cgtcaggtgc ccgcggtcat caatcttcgc gttggcctgc
     1501 gccacgatgt acttgtcttc ctgatcggcg gtgaggaact cgctccgacg ggtgacgttt
     1561 ccatcgcgga ccacgcggta gggtgtttca atgaacccaa ggtcattgac acgcgcgtag
     1621 gtcgcaagcg agctgatcag cccgatgttc ggaccttccg gtgtctcgat cgggcacatc
     1681 cgcccgtagt ggctggtgtg cacgtcacgc acttcgaagc cggcgcgctc gcgcgacaga
     1741 ccgccggggc cgagcgccga cagccggcgc ttgtgcgtca gttcggccag tggatttgtc
     1801 tggtccataa actgcgagag ctgactgctg ccaaagaact ccttgatcgc ggcgaccacc
     1861 ggccggatgt tgatcagcac ctggggggtg atctgctgcg tctcctggat cgtcatccgc
     1921 tcgcggatca cgcgctccat gcgcagcatg ccgatgcgga actggttctg cagcagctcg
     1981 ccgaccgacc gcacccggcg gttgcccagg tggtcgatgt cgtcgacggt cgtgccctct
     2041 tcctcattgt ggagacgaat caggtaccgg acgacctcgg cgatgtcatc gcgcacggcc
     2101 gccggatgac gcacgccttc ctctgagatg acgtacagca cttccttatt aatggaaacc
     2161 aacccgagca cgacccggga ccccttttcc agcttgcgga catcggcggg ctccttgtcg
     2221 attcggaccg tcaccttggc cgtctggccg gtaaactcgc gcccatcgcg cagctcgagc
     2281 gtaaactcct tgcggtcccg ggacaccctg acgtcggcca gctccccgcc ggtcgtctgc
     2341 accagcgtgc ggaatccaag cggagcctcg aggtcgagct tccggttcac cttgtagcgc
     2401 ccaaccttgc cgaggtcata ccggcgcggg tcgaagaaca gcgtctggag cagctggcgg
     2461 gcgctctcca ccgtgggggg atcacccggc cgcagccgcc ggtagatctc gatcagggct
     2521 tcctggtagg ccttctcccc ggcccgctct ttcacgggat cctttgccag ggttgcctgg
     2581 atggtcttgt cgtgctcgta gagcttcagg attttctcat cggagtcgta cccgagcgca
     2641 cgcagcaaca ccgtcacggg aatcttcctg gtgcggtcaa tgcgtgtgct gatgacgccg
     2701 ttgttgtcca gctcgagctc gagccacgcg ccgcgatgcg ggatcaccgt cgccgtcggc
     2761 atcggccggc ccgacgcgtc ctcgccgtag ttgtagtaga cgccggggga tcggacgagc
     2821 tggctgacca ccacgcgctc ggccccattg atgatgaagg tgccgcggcg ggtcatgagc
     2881 gggaggtcgc ccatgaagac ttcctgctct ttgatctccc cagtttcttt aataagaagc
     2941 cggactttga ctttgagcgc cgcgctgtag ttgtaatcgc ggtcccggca ctcctcctcg
     3001 ctgtacttca gccgttccag gcggtagccg ccgaaatcca tgacctggtc gtcgttgaac
     3061 aggctcggtg gatcgggtcg cttgccgcga ccttcgacgg caaagtgcag ctcgaggttg
     3121 ccggtgaaat cctgaatcgg cgacacctcc tcgaagactt cgcggatgcc ctcgcgcaga
     3181 aaccactcgt aggagcggcg ctggacctca atgagatcag ggacgtcgag gatctccggg
     3241 atctttgcga agctttctct ggtacggtgg ccgcgcgtga cgggatgccc gacgatggcc
     3301 gtgcgggact tgccccgcgc acggagaaac ttgcgtcgcg tctgtgtctt cacagggtga
     3361 cattcacctc cgagttggaa ataggaagcc ctatacggct cactgggcta gagtgtggga
     3421 gtgacaggtc ctgtcggatg tctcaggcgt caagaaaaac agcgaaagca aggcatacga
     3481 tgcctcgctt tcggttgtcg gcgtcggatt taactagact gaatcccttt cgacaagtaa
     3541 caaccgtatc ctccatccta atgaggagga tacggggtgt caatacccct gactaccggg
     3601 aacggagaac tgggaacggt aaccgcgggt ttgtgacggt tccccgttac ccgttcgccg
     3661 ttcccgtcat ttgatttcga tcgtcgcccc ttctgcttcg agcttggtct tcatctggtc
     3721 ggcctcggcc tttccgacct tctccttcac cggcttgggc gcaccatcca ccaggtcctt
     3781 ggcttccttg agaccaagcc cggtgagttc tcggacgacc ttgatgacct ggatcttctt
     3841 gtcgccgatg gtcttgagga ccacgtcgaa ctccgtctgc tcctcaaccg cctgagccgc
     3901 cgcgcccgcg ccgccgccgc ccggcattgc cacggccatt ggcgccgccg cggtcacgcc
     3961 gaacttctct tccagcgctt tgacgaggtc ggcaagctcg aggaccgtga gattgccgat
     4021 cgcgtcgacg atctcatcct tgcccagctt actccctgct gccttcgttt tagtttctgc
     4081 cactttcgtc tcccccctta actgactgtc tgcacggtct gcacggcctg cacagtctgc
     4141 acagtctcat actgcggtgc ttgcttttgg cgtatctggt caagtgcgta cacaagattc
     4201 ctctgcaatg cgaccaacac accggccaca cccacgagcg gcgacttcat gttccgcaac
     4261 gccatcgcga gcagctggtt cttggacggc agatcggcaa ggactttgac ctgatcggct
     4321 gtgaggacac ggccttcgac caggccggct ttgacctcga gtttcttcat ctgccgcatg
     4381 tactcagcga ggatcctcgc tggagcaatg ggatcgcggt tgctgaacgc cacagctgtc
     4441 gggccttgaa gatacggctc gagaccgcgg attcccaggc tctccgcggc gcgttcaagc
     4501 agggtgtttt tgaccacttt gtactcggcg ccggcatcgc gcagcttgct gcgcagctgg
     4561 gatatctcgc ccacgttcag tccgcgaaag tcggtcagga tggccgcggc ggcactgtcg
     4621 agccgttcgc gaatcgactc tacctgtcca acctttctcg ggcgcgcccc gcggtcgggc
     4681 tccaccttct taggctgggt tttcctcttc gccatcgtga atcctcacca cgaaaaagaa
     4741 atgggccacc gcagacggga ggccctgaat tctgacactc gcctcggcag ggggccttcc
     4801 ggccattaac gccccgcgcg taagctcggg acgcacctgc tgtctacggc ctgttattca
     4861 tttggtccga aatatagcgg ggcctagtct atcacatgaa atcgacggca cgcaattctt
     4921 tggttcggga acgactaacg gggaactggg aactgtcaac gcatttaccg ttacccgttc
     4981 ccggttccct gttcccgtct ctacgtggtc gcagtcttca gcagcgcctg cgccttggca
     5041 gggtcaatct tgatgcctgg gccgtgagtc gttgaaagcg tgatactcat caggaacggt
     5101 ccctttgacg ccgcgggctt cgcgcgcgaa acagcctcaa tgagcgaagt gaaattctcc
     5161 agcaattggt cttcgctaaa cgacgccttc ccaatggggg catgaattac acccgccttc
     5221 tcggtgcggt actcgatctt tccggctttg aactctttga cggctttgtt caaatcgaat
     5281 gtgaccgtcc cggacttggg gtttggcatg aggccacgcg gtccgaggat gcggccgagc
     5341 ttcccgaccg tgctcatcat gtctggggtc gccaccgcca cgtcgaaatc agtccagccg
     5401 ccttgaatct tctcgatgaa ctcatcggct ccgaccatgt ctgcgccggc cgactcggct
     5461 tcctttgcct tctctccctt ggcaaacaca agcagccgga ctttctttcc ggtccccgcg
     5521 ggaagcgcga cggtcccgcg cacctgctga tcggcctgct tgggatcgat gcctaggcgg
     5581 atggccacct ccaccgtctc gtcaaacttg gccttcggcg cctgcttcaa gatcttgatc
     5641 gctcccgtag gatcgtggag ctgatcttca ccggcgagct tggcagcgtc ccgatatcgt
     5701 tttccgtgtc gtttcttcat ctcaactcgt cacctcaatg cccatcgacc gggccgtccc
     5761 ctcgatcatc cgtacggcgg catccaggtc gttggcgttc aaatccttca atttcttctc
     5821 tgcgatgcca cggacttgcg cccgtgtgac tttcccgact ttgttcttgt tgggttggcc
     5881 tgagcctttt tccagcccgg cggcctgccg aagcaggaca gatgccggtg gtgtcttggt
     5941 aatgaacgag aaagtacgat cggcgaacac cgtgatctca accggcacga tcgtcccagc
     6001 ctgcgacgcg gtgcgctcgt tgtacgactt acagaactcc atgatgttca cgccatgctg
     6061 gccgagcgct ggcccgacag gtggcgccgg cgtcgctttg ccggcgggaa tctgcagttt
     6121 gacgatcgcg ataactttct ttgccatgcc ctgatccctt cagacctttt ctacgtctcc
     6181 gaagttcagt tcgaccggtg tggaccgtcc gaagatcgag acaaggacgc ggaccttttc
     6241 cttctcgacc atgacatcgt caacctgacc cgtaaagtcc atgaatggac cggtggtgat
     6301 tctcaccatc gagcctttct ggaagttgat gcggtacttg ggcgtttcgt catggatctg
     6361 cttgaggagg cgtttgactt cctgatcctc caacgcgacc ggccgcgcac ccgatccgac
     6421 aaaacctgtg actcccggcg tattgcggac gacgtaccat gagtcgtcgt ccatgaccat
     6481 ctcaaccagg acgtaacccg gataaatctt gcggtcgact tcgcggcgtt tacctccctt
     6541 gatttccttt tctttctcgg tgggaacgag cacctgggcg atcttctcgc cgcccttgcc
     6601 gtgcatcgtc ttggtccggc gttccaggtt cgtcttgacc ttgttctcat aaccggagta
     6661 ggtgtggatc acgaaccacc gcaggcgggg gtcacgaggg gcttctggct ctgtcggcgc
     6721 ctcggcttct ggcgcttcga cggcggcctc accattaggc tcgacttgca cttcgagctc
     6781 agcccttgcg gccggctcct cgacgacagc cggctccggg tcagacaccg gtgcgggcgc
     6841 aggctcaggt tcggcgaccg gttcgtcgcc aaacaattct ttgatcttgt cctcgatcac
     6901 cggtcttttg tcttccatat ataggtgcct ctactgccgg actaagcccc gctggaacag
     6961 ccaggtgaat aggaggtccc atgcgccgag ataaagcgac atcagcagca gaacgacgac
     7021 aacgacgatc gtcattgccg tcagttctgc gcggctcggc cagtcgactc gtcccagctc
     7081 ggttcgcacc tcgcgaaggt atcgacgtat ccgctcaata atgggaaggc ccgtgcgcac
     7141 gggcgcgggc tttgccgcag ggcggaccgc ctgccgg
//
LOCUS       DBNGSB010000062         3115 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_193494, whole genome shotgun sequence.
ACCESSION   DBNGSB010000062 DBNGSB010000000
VERSION     DBNGSB010000062.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_193494"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="AC159B_01750"
     CDS             <1..1107
                     /locus_tag="AC159B_01750"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3376512.1"
                     /translation="AAEIRPHAGGWDRDGTFPLALVPKLAALGLFGLVVPAEHEGAGL
                     DMVSAMLAVEAICWGDGAIGLTVASHNSLCTGHVLLFANEEQKRRYLPALARGEALGA
                     WCLTEPGAGSDAASIQTRARRSGSAYVLNGSKAFVTQGSIGGVYVVMAVTDPAAGREG
                     ISAFIVERGAQGLRIGKKEDKLGVRACDTAEVTFEDCEVPASALIGREGAGYAQAMAI
                     LERGRIGIGAMAVGLGRAALDDSLAYASQRQTFGKPIAQHQAIQFMLADMGTELDAAW
                     LLVLRAAQLVDLGRPFRREASMAKLFASEAGARATNKAVQIHGGYGFIKGYAVERYYR
                     DIKLCEIGEGTSEIQRIIISKSLIERRIDPITRS"
     regulatory      1134..1239
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            1281..>3115
                     /locus_tag="AC159B_01755"
     CDS             1281..>3115
                     /locus_tag="AC159B_01755"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HIR3376513.1"
                     /translation="MTHPFLERLNQGVLLADGAMGTVLYARGVPFDRCFDELNLSAPE
                     IVRDIHRDYIASGAELIETNTFGANRFKLATHNLERRFAEINRAGAALARDAVQASGR
                     SVLVAGSMGPIGRPIAPLGTVTPEEVRDAFAEQAAALVAGLADVLILETFTDLAELVE
                     AVRGVRSATGVPLIAQMTFTQEGKTLLGHEPREIVQTLEGLNVDVVGANCSVGSQGIL
                     DVMTVMAEVATTAKLSAMPNAGFPAYLGGRYIYFSTPAYMAEHARQLVALGVRVVGGC
                     CGTTPEHIAAMREALASPSAEPAAAVRPIARPHEPKVEITAPAEPTPLAQKLGKSFVV
                     SVEIDPPRGIHDRKELEGCRRLAQAGVDAVDVSDNPMARLRMSPWAMCARIQGEVGLE
                     TILHFTTRDRNLVRLQSDLLAVHALGVRNILVLRGDPPQMGDYPDATAVSDVKPSGLV
                     RLVKDFNRGRDVAGNSIGAPTTFTVGVALNPSAPDLDKEAKVLERKIQAGADFICTMP
                     VFEAEALDRFIRLLGGLPVPLLMGLMPLASHRHAEFLHNELPGLSIPKGVRLRMRAAP
                     DGRDEGVRIAAELLSAVRHRVAGLYLVPSFGRYDAVIELVKLSKER"
BASE COUNT          556 a          938 c         1060 g          561 t
ORIGIN      
        1 gcggctgaga tccgccctca tgccgggggg tgggatcgtg acgggacctt tccacttgcg
       61 ttggtgccaa agctcgcggc cctcggcctg tttggactgg tggtcccggc ggagcacgaa
      121 ggcgcgggcc tcgatatggt cagcgcgatg ctggccgtcg aggcgatttg ctggggcgat
      181 ggcgctatcg ggctgacggt tgcctcgcac aactccttgt gcacgggcca cgttctgctc
      241 tttgcgaatg aggagcagaa gcgccggtac ctgccggccc tcgcccgagg cgaggcgctc
      301 ggcgcgtggt gcttgaccga acccggcgcg ggcagcgatg cggcgtcaat ccagacgcgg
      361 gcccggcgga gcggcagtgc ctacgttctc aacggctcga aggcctttgt cacccagggc
      421 agcatcggcg gtgtgtatgt ggtgatggcg gtaacggatc ccgccgcagg cagggaaggg
      481 atcagcgcgt tcattgttga gcgtggagcg caggggttgc ggatcggtaa gaaagaagac
      541 aaacttggtg tccgggcgtg cgacacggcg gaggtcacgt tcgaagactg cgaagttccc
      601 gcgtccgcat tgatcggccg cgaaggcgcg ggctatgctc aggccatggc gatcctcgag
      661 cgtggacgga tcggcatcgg ggcgatggcc gtggggctcg gtcgggcggc gctcgacgat
      721 agtcttgcct acgcgagcca gcgccagacg ttcggaaagc cgattgccca gcatcaggcc
      781 atccagttca tgctggccga catgggtacc gagctcgacg ccgcctggct cctcgtgctg
      841 cgggccgcgc agctcgtgga cctaggtcgc cccttccgcc gcgaggcgtc catggccaag
      901 ctcttcgcct cggaggccgg cgcacgcgcg acgaacaagg ccgtgcaaat acacggcggc
      961 tatggattca tcaagggcta cgccgtggaa cgctactacc gcgatatcaa gctctgtgag
     1021 atcggtgaag gaacgtctga aattcagcgg atcattattt cgaagtctct gattgaaaga
     1081 cggattgacc cgataacccg ctcgtgatat acagatgaca atcgaatatg gcgctcttat
     1141 cgagagtggg tgagggacgg gcccgatgac ccccggcagc ctgcagaacg cctaacggca
     1201 aggtgctaaa tcccgcggac caattccgag agataagagg gaaaagcgcc cctccaggag
     1261 ttgggagggg tgttgctttt atgacgcatc cattcttaga acgcctcaac cagggtgtgc
     1321 tgctcgcgga cggcgcgatg ggcaccgtgc tgtatgcccg cggtgtgccg tttgaccgct
     1381 gtttcgatga gctcaatctg agtgcgccgg agatcgtcag ggatattcac cgggactata
     1441 tcgcatcggg cgcagagttg attgaaacga acaccttcgg agccaatcgg ttcaagctcg
     1501 cgacccataa tctcgagcgc cggttcgccg agatcaaccg ggcgggtgcc gcgcttgcgc
     1561 gagacgcggt ccaggcatcg ggccgctccg tccttgttgc cggctcgatg ggccccatcg
     1621 ggcggccgat cgcgccgctg gggaccgtga ctcccgaaga ggttcgggat gcgtttgccg
     1681 agcaggcggc tgcgctcgtt gctggtctcg cggacgttct gatcctcgag accttcaccg
     1741 atctcgccga gctggtcgag gcggtccgcg gcgtacgctc agccaccggc gtgccgttga
     1801 tcgcccagat gaccttcacc caggaaggca agacgctgct gggacacgag ccgcgcgaaa
     1861 tcgtgcaaac cctcgaagga ctcaacgtcg acgtggtggg cgcgaactgc agtgtgggat
     1921 cacaggggat tctcgatgtt atgacggtga tggccgaagt tgcgacgacg gccaaactgt
     1981 ctgcgatgcc caacgcggga tttccagcct acctcggcgg ccgctacatt tacttctcaa
     2041 cgccggcgta catggcggag cacgcacgac aactggtcgc actcggcgtg cgcgttgtcg
     2101 gcgggtgctg cgggaccacg cccgagcaca tcgccgcgat gcgcgaggcc ctggcgagtc
     2161 cgtcggcaga accggcagcc gccgtccgtc ccatcgcgcg tccgcacgaa cccaaggttg
     2221 agattacggc tcccgccgaa ccgacgcctc tggcgcaaaa actcggcaag agtttcgtcg
     2281 tcagcgtgga gatcgatccc ccgcgcggta tccacgaccg gaaggagttg gagggatgcc
     2341 gtcggctggc gcaggccggc gtggacgcgg tcgacgtgtc ggacaatccc atggcccgcc
     2401 tacggatgag cccatgggcg atgtgcgcgc gcattcaagg cgaggtggga ttggagacca
     2461 tcttgcactt tacgactcgt gacagaaatc ttgtccgact gcaatcggac ctgctggcgg
     2521 tccacgcgct gggcgtgcgg aacattcttg tgctgcgcgg ggatcctccg cagatgggag
     2581 attatcccga cgcgacggcg gtctccgacg tcaagccatc ggggctcgtc cggctggtca
     2641 aagatttcaa tcgggggcgt gacgtcgcgg gcaattcgat cggcgcgccg actacgttca
     2701 cggtgggcgt ggccctgaac ccgtccgcac cggatctcga caaagaggcg aaggtgctcg
     2761 agcgtaagat ccaggccggc gcagatttca tttgcaccat gccggtattc gaggcagagg
     2821 cgctcgaccg gttcatccgc cttctgggcg ggctgcccgt gcccttgttg atgggactga
     2881 tgccgctggc cagccaccgc cacgccgaat tcttgcacaa cgagctgccg ggtctcagca
     2941 tccccaaggg cgtccgactc cggatgcgcg cggcgccgga tggcagagac gaaggagttc
     3001 ggatcgcagc agaactgctg agcgcggtcc gacatcgcgt ggcgggcctc taccttgtcc
     3061 catcgttcgg acggtacgac gcggtgatcg aattggtgaa gctttcgaaa gagcg
//
LOCUS       DBNGSB010000063         5378 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2035200, whole genome shotgun sequence.
ACCESSION   DBNGSB010000063 DBNGSB010000000
VERSION     DBNGSB010000063.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 5378)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5378)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5378
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2035200"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..628)
                     /locus_tag="AC159B_01760"
     CDS             complement(164..628)
                     /locus_tag="AC159B_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013913470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376514.1"
                     /translation="MLNGRAIWCLDHGRPIAAQACTRVPGRYVSRLFFIMTLSFTAVV
                     AADASRDLRAQAPKATIVGPGAATCQRFNDDIKSNPLLRRDYLAWAQGFMSGILSSRP
                     PGVDEGLNLAPMTFDVRRQLQFLEDYCTRNASKDFSDAVEALYTRLREEGKI"
     gene            complement(643..1014)
                     /locus_tag="AC159B_01765"
     CDS             complement(643..1014)
                     /locus_tag="AC159B_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376515.1"
                     /translation="MRSPLLLIATVLFAVAVSAEEYKRGPNGGLMLDVAGVDAELMTA
                     GKTVTINVFVPNTAKPVPTDRFTAAALIVSGSNRETISLTPRGENSLKGDAKNPIAPD
                     ATITLTIKTAEEKSGQVKFKK"
     gene            complement(1011..1271)
                     /locus_tag="AC159B_01770"
                     /pseudo
     CDS             complement(1011..1271)
                     /locus_tag="AC159B_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022549.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
     gene            complement(1309..1572)
                     /locus_tag="AC159B_01775"
     CDS             complement(1309..1572)
                     /locus_tag="AC159B_01775"
                     /inference="COORDINATES: protein motif:HMM:NF013629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIR3376516.1"
                     /translation="MTTPIEMQFRAKGLRLTKQRRLIVEAISEANGCPDFAELYRRAA
                     GRNRRVSRATVYRALRLLTSEGIIECYTFRGGRPRFGSLGGAS"
     gene            1707..2825
                     /locus_tag="AC159B_01780"
     CDS             1707..2825
                     /locus_tag="AC159B_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007787964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HIR3376517.1"
                     /translation="MRTPIWQRLSRRRVLSGGIVAAGGAIVGALPNGRPAVAHPPDAS
                     HGANVATTATHASANPMITVGDVGSAKNGFDPTAMLTDWETGTVSQLPDGRTLRTFEI
                     EGQDKEIEIASGVLFPAWTYNGRVPGPTLRATEGDHLRVVFRNRASHPHSMHFHGIHA
                     ARMDGVPGAGLIEPGQEFVYEFDALPFGCHLYHCHALPLRRHISKGMYGAFIVDPDPA
                     RHPESVAVAQSRLLGTAENKEWQELVMVMNGFDTNFDRKNETYAVNTIAHHFMKHPIR
                     IDHDRPVRIYLVNVLEFDLINSFHLHGNFFDYYDHGTTLIPTLTTVDTVMQCQAKRGI
                     LEFTYRKHETGAYMFHAHQSEFAELGWMGLFDVVAVAS"
     gene            2822..4039
                     /locus_tag="AC159B_01785"
     CDS             2822..4039
                     /locus_tag="AC159B_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011578861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIR3376518.1"
                     /translation="MIEIIRPQAQMPGAASNAQPPRSYTKLLWFALPLFALVVAAAWL
                     VASDWLKVFDTGAPPVEKLTFERVILDGQGIHLKVRAGGSEPMMIAQVTVDEAFWSFR
                     QEPPGPLDRMSTAWLHIPYSWVLGEKHDVGVLTKTGLTFKKEIEVAIATPQAQVGQLV
                     PQALLGAFVGVVPVIIGMLFYPAMRSLGRQGLDFVLALTVGLLAFLFVGTLKDALAFA
                     AQAAPAFQGIAMVLVVAAIAFLTLFVVGRRHGTPTGLTLATYIALGIGLHNLGEGLAI
                     GAAFAAGVAGLGGFLVLGFTLHNVTEGIGIVAPLVVSRPSLPAFAGLAILAGAPAILG
                     IWLGSLSYSPHWSALALAIGAGAILQVIVEVGAYLFRIASERGATWLSSGSLGGLIVG
                     FVVMYATAMLVKI"
     gene            complement(4292..4531)
                     /locus_tag="AC159B_01790"
     CDS             complement(4292..4531)
                     /locus_tag="AC159B_01790"
                     /inference="COORDINATES: protein motif:HMM:NF012624.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HIR3376519.1"
                     /translation="MGGIRDRRKNLATRRRERLILDGFPRTVFQAAALDDVLLIEERE
                     LSCVLELKVDERILLDRILGRAVQAKGQRRAGESR"
     gene            complement(4629..4865)
                     /locus_tag="AC159B_01795"
                     /pseudo
     CDS             complement(4629..4865)
                     /locus_tag="AC159B_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470527.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
     gene            4906..5118
                     /locus_tag="AC159B_01800"
     CDS             4906..5118
                     /locus_tag="AC159B_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376520.1"
                     /translation="MAVLQEAGAIRECEEQGWMQDRVDPHARERAFAIARQDPPTGVS
                     PQAAAVAIAEVLESIGDTCPGCPLEG"
     gene            complement(5122..5325)
                     /locus_tag="AC159B_01805"
                     /pseudo
     CDS             complement(5122..5325)
                     /locus_tag="AC159B_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688949.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
BASE COUNT          998 a         1646 c         1611 g         1123 t
ORIGIN      
        1 gaacttcgtc ccgaagtcct gacgctctgc ggggcgctgt cgaagcgact tggctggcga
       61 ggcgcttcat cgtgacgccg agccatccgg cgtaagcgcg tcttcgcgga tgaggcggtg
      121 ctattcatgg tcgcttcctc aggcaaggcg ggagaggtcg agttcatatt tttccctcct
      181 cgcgcagccg cgtgtagagg gcttcaactg catccgagaa atcctttgag gcattccgtg
      241 tacagtagtc ctccaagaac tgcagttgcc tcctgacgtc gaacgtcatc ggagcgagat
      301 tgagcccctc gtcaacaccg ggcggcctgc tcgacaggat tccactcatg aacccttgcg
      361 cccaggccag atagtctctc ctgagcaagg gatttgattt tatgtcatcg ttgaaccgct
      421 ggcatgtcgc agcacccggg ccgacaattg tcgccttggg cgcctgcgcg cgaagatcgc
      481 gcgatgcgtc cgccgccacg acggcggtga aggacagcgt catgatgaag aacagacgac
      541 tgacgtatcg accgggcacg cgcgtgcagg cctgcgccgc aattgggcgg ccgtggtcga
      601 ggcaccagat tgcacgtcca ttcaacatcc tgccgtcggt ccttacttct tgaactttac
      661 ctgacccgat ttctcctcgg cggttttgat ggtgagggtg atcgtagcgt ccggcgctat
      721 cgggttcttc gcgtcgccct tcagtgaatt ctcaccccgc ggcgtaagtg aaattgtttc
      781 gcgattactg ccgctgacga tgagcgccgc tgccgtgaaa cggtctgtcg gaacgggctt
      841 cgcggtgttt ggaacaaaga cgttgatcgt gaccgtctta cccgcagtca tcagttcggc
      901 atcaacaccg gcaacgtcga gcattaggcc gccgttggga ccccttttgt actcttcggc
      961 ggagaccgca actgcgaaca agacggtagc aatcaacagc agcggggatc tcatgagggt
     1021 gctccctctt gggcgggtga tgtcgcttca ccgttcgcca gatcctcccg cctgaacaga
     1081 acgtagagcg ccggcagtac cagcaacgtg aggatggtcg atgagatgat tccgccgatg
     1141 acgacggtgg cgagcggccg ctgcacctcg gcgccggcgc cagtcgcgac cgccatcggg
     1201 acgaagccga gcgacgcgac cagtgccgtc atcagaaccg gcctgagacg ggtcagggcg
     1261 ccatcgagaa ttcgacaaat gttgccggta tccaagtcga tcaggggttc atgatgctcc
     1321 ccccaaggaa ccgaagcgag gccgaccacc tcgaaacgtg tagcactcga tgatgccttc
     1381 gctcgtgagc agccgcaaag ccctgtaaac cgtcgcccgc gacacgcggc ggttgcgccc
     1441 cgcagcgcgt cggtacagtt cggcaaagtc tgggcaacca ttggcttcgg agattgcttc
     1501 gacaatcaga cgccgctgct tggtcagccg gagacccttc gcacggaact gcatctcaat
     1561 cggagttgtc atggcttcaa gcccgccatg gcgggccgat cttgcctacc ctcttgcaat
     1621 gtctcacaaa aaggagaaag atgcaactcg tttgcaatcg caactggact tttgttgcta
     1681 cgatacccgc gaggcgggag gcgtccatgc gaacaccgat ttggcagcgg ctaagccggc
     1741 gaagagtact ctcaggcggc atcgtcgctg ccggcggcgc gatcgtgggc gctctgccaa
     1801 acgggaggcc ggcagtcgcc cacccccccg atgcatctca tggcgcgaac gtagcgacta
     1861 ccgccactca tgcttccgct aacccgatga tcacggttgg cgatgtcggt agcgccaaga
     1921 acggcttcga tccgaccgcg atgctcaccg actgggagac tggaacggtg tcgcagctgc
     1981 cggacggacg cacattgcgg accttcgaga tcgaaggaca ggacaaggaa atcgagatcg
     2041 cgtcaggagt gttgtttccg gcttggacgt ataacgggcg cgtgcccggt ccgacgctgc
     2101 gtgccaccga gggcgatcat ctgcgcgttg tgttccggaa ccgtgcctcc catcctcatt
     2161 cgatgcactt ccatggcatc catgctgccc gcatggacgg ggtgcccggt gccggtctga
     2221 tcgagccggg ccaggagttc gtctacgagt tcgacgcact gccgttcggc tgccatctct
     2281 atcattgcca cgcgctgccg ctgaggcgtc acatcagcaa gggcatgtac ggcgccttca
     2341 tcgttgatcc tgatccagca cgccatcctg agagtgtggc cgtcgcgcag tcgcgcctgc
     2401 tcggcaccgc cgaaaacaaa gaatggcaag agctggtgat ggtgatgaat ggcttcgata
     2461 cgaatttcga taggaagaat gagacttacg ccgtcaacac gattgcgcat catttcatga
     2521 agcatccgat ccgcatcgac cacgaccggc cggtgcgtat ctatctggtg aatgtgcttg
     2581 agtttgatct gatcaactcg tttcatttgc acggcaactt ctttgattac tatgaccacg
     2641 ggacgacgct catcccaaca ttgacgacgg tcgacactgt catgcagtgc caagccaagc
     2701 gtggaatcct tgagtttacg taccggaaac acgaaaccgg cgcctacatg ttccacgcgc
     2761 accagtccga atttgcggag cttggctgga tgggactgtt cgacgtcgtc gcggtggcgt
     2821 catgatcgag atcatccgcc cacaagcgca aatgcccggg gctgcgagca acgcacagcc
     2881 gccccgctcc tataccaagc tgctgtggtt tgcattgccc ctgttcgcgc tcgtcgtggc
     2941 agcggcctgg ctcgttgcaa gcgactggct caaggtcttc gacactggag caccgccggt
     3001 cgagaagctc accttcgagc gcgtgatcct cgacggccag ggaatccatc tcaaggttcg
     3061 cgccgggggt tccgagccga tgatgattgc ccaggttacg gtcgacgaag ccttctggtc
     3121 gttcaggcag gagccgcccg ggccgctgga tcgcatgtcg accgcgtggc tccatatccc
     3181 gtattcgtgg gtgctaggag agaagcacga cgtcggggtg ctcaccaaga caggcctgac
     3241 cttcaagaag gagatcgagg tcgccattgc aaccccgcag gcgcaggttg gccagctcgt
     3301 tccccaagcc ctcctgggag ctttcgtggg cgtcgtgcca gtgatcatcg gcatgctgtt
     3361 ttacccggcg atgcgcagct tgggacggca gggcctcgac ttcgtcctgg ctctgaccgt
     3421 tgggctcctt gcatttctgt tcgtgggcac gctgaaagac gcgctggcat ttgccgctca
     3481 agcggcgccc gccttccagg gtattgcaat ggtactcgtg gtcgctgcaa tcgcgtttct
     3541 cacgctgttt gtcgtcgggc gccgccacgg aacgccgacc gggctcacac ttgccaccta
     3601 tattgccttg gggattgggc tacacaatct cggtgaaggt ctcgcgatcg gcgcggcgtt
     3661 cgcggcgggc gttgccgggc tgggtggctt tctcgttctc ggtttcaccc tgcacaacgt
     3721 cactgaagga atcgggattg tggctcccct cgtcgtgtct cgcccatcgc tgccagcctt
     3781 cgcggggctg gcaattctcg caggagcgcc cgcgatccta ggcatatggc ttgggagctt
     3841 gagctattcg cctcactggt cggcgctcgc actcgccatc ggcgccggag ccatcctgca
     3901 agtcatcgtc gaggtgggtg cctatctatt ccgcattgcc agtgaaaggg gtgcaacgtg
     3961 gctctcgtcg ggctcgctag gcggtctcat cgtcggtttc gtcgtgatgt atgccacggc
     4021 catgctagtg aaaatataac gcgtggagaa tgcaggtcga ggagccgaca atccgtcagg
     4081 tcgatgagac taccactacg gcgcaggctc cggcccttaa gcggagtcat ctacgggtga
     4141 ttcgatcgcg tcaatcagcg ctgccgtgac acgatcgatc gcctgggaag catcgatggt
     4201 cctcaacagg cccttggcgc gatagtattc ggttaggggt ttcgtttggt tgccgtaagc
     4261 ggtcaaacgc acgctgagag cttcctcatt gtcatctgct ctcaccggct ctccgttggc
     4321 cctttgcctg cacggcccgc cctagaattc gatccagcaa gatcctttcg tcaaccttga
     4381 gttcaagcac acaactgagt tcccgctcct cgatcagaag aacgtcgtcg agcgcagctg
     4441 cctggaagac cgtgcgcgga aatccatcca ggatgagcct ctcgcgacgt cgggttgcga
     4501 gattctttcg acgatcacgg ataccaccaa ttgatctggg accaaatttt ttgagataat
     4561 ctgagcgctt gggttccttt gcctgctcct ggcggtccta tgagtacgac tctcatccgg
     4621 ttattccgtc aagcagatgt tcgggcagcg gcgcgcgaaa tcgcatcaat caacctgcgc
     4681 gcttcatcgg cgtgccccca tcccgtaagc ttcacccact tgcccggttc tagatccttg
     4741 tagtgaagga agaagtgctc gatttgcttg cacgtgattt ccggcagatc ggtaaaggtc
     4801 ttcacatgct gatatcgctg agtaagcagt gacgacggaa ccgcgacgat tttctcgtcg
     4861 ccgccgcccc acgccgccgt gatcctaacc ctacggagct gggcgatggc ggtgctgcag
     4921 gaagccggcg ccattcgcga gtgcgaggag cagggctgga tgcaggatcg cgtcgatccg
     4981 cacgcacgcg aacgcgcctt cgccatcgcc cgccaggatc cgccgaccgg tgtttcgccg
     5041 caggcggcgg ccgtcgcaat cgccgaggtg ctcgagtcga tcggcgacac ctgcccgggg
     5101 tgcccgctcg agggatgagc tctattcgcg aaggattgtc cgcgacgtct ccaggccgct
     5161 cgccagcgcc gcctcgaccg tgcccatgtc tcgcacgcgg tagaatgctt cgccggaaaa
     5221 gaaaaccaac ccgccatcat ttcttgaaaa caccgactgc gccgcgcgcg tttccggtgt
     5281 cgtgtacgga taggctccgc ccgccagagg atcgtgcgcc cagttgattg gctctctcgt
     5341 cgcctcagag gccgccgcta tttagttgcc tgaatggg
//
LOCUS       DBNGSB010000064         2874 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3875778, whole genome shotgun sequence.
ACCESSION   DBNGSB010000064 DBNGSB010000000
VERSION     DBNGSB010000064.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3875778"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="AC159B_01810"
     CDS             complement(<1..603)
                     /locus_tag="AC159B_01810"
                     /inference="COORDINATES: protein motif:HMM:NF016051.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase
                     domain-containing protein"
                     /protein_id="HIR3376521.1"
                     /translation="MALDKKKLLITSGPTRAPLDAVRYISNKATGKLGAIIAEGALQR
                     GADVTYVYGRPGELPVARGGRDHLRLIPVETVEDLVAVFREELPKRYDAVVHPMAVLD
                     FQPDSAREYKTSSDTEEWVIRLVPTAKAIRLVKDLAPDTFLVGFKLEIGKEPRELQSI
                     ALEFLKRNRCDLVIANDLKEIEAGRHTGYFIMPDGKVAKTA"
     gene            complement(610..1191)
                     /locus_tag="AC159B_01815"
     CDS             complement(610..1191)
                     /locus_tag="AC159B_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376522.1"
                     /translation="MIKVHVNVRHLASGTAFGTLSEERERLTQILATGLPTGLVPGET
                     EMVAPEEMLELKKMKFAFLDAFVDSIRPYLYDAGIPVVPALPHSAERQFLTRARDLPG
                     DWVEAAAVDSHGYGKPPADSDFATLRTVGTTTKKHLIVPTQRRISPGDVPRYFEIPQV
                     AAIMIGAIVTGKAPGSLGRATGEFRRELDRLFA"
     gene            complement(1281..2603)
                     /locus_tag="AC159B_01820"
     CDS             complement(1281..2603)
                     /locus_tag="AC159B_01820"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018834706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIR3376523.1"
                     /translation="MREQDSEVFRILEAERRRQREGVELIPSENYVSAAVLEAMGTIF
                     TNKYSEGYPGRRYYGGNEHVDEIERLAQKRAMQLFSVPHANVQPYSGSPANLAVYLAT
                     CQPGDVIMGQNLPDGGHLTHGWKVSATGMYYRSVPYHVKADGYIDFDDVWRLAREHRP
                     RLIWCGATAYVREFPFEKFAEIADELGAFFAADIAHIAGLVIADAHKSPVPYAHIVTT
                     TTHKTLRGPRGAMILVTQRGLEKDDELGERIDRAIFPGLQGGPHDHTTAAIAVALGEA
                     MQPEFKEYGRRVVANSKALAGGLMKRGFKLVTNGTDNHMMLVDLSPGGPGRGVFMQEA
                     LDRVGITVNKNTIPGEPSSPFYPSGVRLGTPAATTRGMREQEMDRIAAWIALVAEEIE
                     ASRLPVDAKARSATLKSFRQAIEENRRLATLRSEVREFCLKFPVPGTT"
     gene            complement(2625..>2874)
                     /locus_tag="AC159B_01825"
     CDS             complement(2625..>2874)
                     /locus_tag="AC159B_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3376524.1"
                     /translation="EYYSIPYRCLLPKGLDNVLVAGRCISSSHEAFAAFRVSAIVMAI
                     GQAAGTAAAMAAARSALPRDVDVSQLQRVLREQGAFLP"
BASE COUNT          529 a          875 c          892 g          578 t
ORIGIN      
        1 ggccgtcttg gccaccttcc catccggcat gatgaaatag cccgtgtgcc ggcccgcttc
       61 aatttccttc aagtcgttgg cgatgacgag gtcgcatcgg tttcgtttga gaaactcgag
      121 cgcgatggac tgcagctctc tcggttcctt gccgatctcc agtttgaatc ccaccagaaa
      181 cgtatcgggc gccagatcct ttacgagtct gatcgccttg gccgtcggca ccaagcgaat
      241 cacccattct tcggtatctg agctggtctt gtactctcgc gccgaatccg gttgaaaatc
      301 gagcacggcc atcgggtgga caaccgcgtc gtagcgtttg ggcaattcct ctctaaacac
      361 ggcgaccaaa tcctcgaccg tttccacagg gatcaaacgc aggtggtcgc gtccaccgcg
      421 ggcgacgggt aattcgcctg ggcgtccgta gacatacgtc acgtcggcgc cccgctgcag
      481 ggcgccttcc gcgatgatcg cgccgagttt gccggttgcc ttattggaaa tgtagcgaac
      541 tgcgtccaga ggagcacgcg tcggaccgga ggtgatgagg agcttcttct tgtcgagcgc
      601 catgtgatgc taggcgaaga gacgatccag ttcccggcgg aattcccccg ttgcgcgtcc
      661 tagtgaaccg ggagccttgc ctgtgacgat ggccccgatc attatcgctg cgacctgggg
      721 aatctcaaag taccgcggca cgtctccggg tgaaatgcgg cgctgcgtgg gtacgatcag
      781 gtgcttcttg gtcgtcgtcc ccactgtgcg caatgttgca aagtcagaat ccgcgggcgg
      841 ctttccataa ccgtgactat cgacggcagc ggcttccacc caatcgcctg ggaggtcccg
      901 cgccctggtc aggaactgac gttccgcgga gtggggaagc gcgggcacga cgggaatccc
      961 ggcgtcatac agatagggcc ggatggagtc cacgaatgcg tccaggaaag caaacttcat
     1021 tttcttgagt tcgagcatct cctccggcgc gaccatctcc gtttcccccg gcaccagacc
     1081 ggtgggaaga ccggtggcaa gaatctgcgt gagccgctct cgctcttcgg acagtgtccc
     1141 aaacgccgtc ccggacgcga gatgacgaac attgacatgg actttaatca tgtctgcgcc
     1201 ggcatttcga gccgcctgag cgagggcggg atcattactc ggaagactta caatgaggag
     1261 gccgcggcga tcgggaaggg tcatgtggtt cccgggaccg ggaatttcag gcagaattcg
     1321 cgcacctcac tgcgcagcgt ggccagccgc cggttttctt caatcgcctg cctgaacgac
     1381 tttagcgtgg cagaccgggc tttggcgtcg acgggcaacc tcgacgcctc gatttcctcg
     1441 gccacgagag caatccaggc cgcgatccgg tccatctcct gttcacgcat ccctcgcgtc
     1501 gtcgccgcag gggtccccag gcgtacgccg gacggataga acggtgagga tggctcgcct
     1561 ggaattgtgt tcttgttcac ggtgatcccc acccggtcga gcgcttcctg catgaacaca
     1621 ccgcggcccg gcccgcccgg ggagagatcc accagcatca tgtgattgtc tgtgccattg
     1681 gtgaccagct tgaacccgcg tttcattaag ccgccggcca gggccttgga gttcgcgaca
     1741 acacgccggc cgtattcttt gaactcaggt tgcatcgctt cgccgagcgc gacggcgatc
     1801 gcggccgtcg tgtgatcgtg cggtccgccc tgaagaccgg gaaagatcgc gcgatcgatc
     1861 cgttcgccga gctcatcgtc cttttccagg cctctctgcg tgacgaggat catggcgccg
     1921 cgcggaccac gcagcgtctt gtgggtcgtc gtggtcacga tgtgagcgta gggaaccggg
     1981 ctcttgtgcg cgtcagcgat caccagacca gcgatgtggg cgatgtcggc ggcgaagaac
     2041 gcgccgagct catcggcgat ctcagcaaat ttttcgaagg gaaattcacg gacgtaggcc
     2101 gttgcgccac accagatcaa gcgcggccgg tgctcgcggg cgagccgcca gacgtcgtcg
     2161 aaatcgatgt agccgtcggc tttaacatga taaggaacgc tgcggtagta catgccggtg
     2221 gcactgacct tccacccgtg ggtgagatgg ccgccgtcgg gaaggttctg gcccatgatg
     2281 acatcacccg gctgacacgt ggcgaggtac actgcaagat tggccgggct gccggagtac
     2341 ggctgaacgt tggcgtgtgg gacactaaag agctgcatag cccgcttctg ggcgagccgc
     2401 tcgatctcgt cgacgtgttc gttgccgccg tagtaccgcc gcccgggata gccttccgaa
     2461 tatttgttgg tgaagatcgt gcccatggct tcgagtactg ccgctgagac gtagttctca
     2521 gacgggatga gctcgacgcc ttcacgctgt cggcggcgct ccgcttccaa gatgcggaag
     2581 acttcggaat cttgctcacg catttccatt tcgttcccgt ttcttcaggg gagaaacgcg
     2641 ccctgctctc ggagcacgcg ctggagttga gagacatcga catcgcgcgg aagggcactt
     2701 cgcgcggccg ccatcgccgc ggcggtgccg gccgcctgcc cgatggccat cacgatcgcg
     2761 gacacgcgaa aggcggcaaa cgcttcatgc gacgaagaga tgcaccggcc agccacgagc
     2821 acgttgtcca atcccttggg cagcaggcac cggtagggga tcgaatagta ttcg
//
LOCUS       DBNGSB010000065         4244 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2648995, whole genome shotgun sequence.
ACCESSION   DBNGSB010000065 DBNGSB010000000
VERSION     DBNGSB010000065.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4244)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2648995"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="AC159B_01830"
     CDS             <1..662
                     /locus_tag="AC159B_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HIR3376525.1"
                     /translation="SNIGVSTFYMLTSTMTNELVKEKGYYPVGGCGANIAWHTEDDTL
                     EIADRDHLLRDIRVYAAAAFRAANSPIYPLDFAATAAEFIRTLDRYQEAAGEAFDLQP
                     VIEEARGLENSLARFYDSAHQSSPRSVNDPDVRRINEAQRRLARVLVPINYSRAGRFR
                     HDPAIDVPPLPDLEPATKIASLAQESDAYRLTRTHLARGRNRVVAALREARRIIEDAQ
                     A"
     gene            666..1616
                     /locus_tag="AC159B_01835"
     CDS             666..1616
                     /locus_tag="AC159B_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016202258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3376526.1"
                     /translation="MHRYIVRRLLIMVPILLGITVLSYLILSLTPGDPVQMLIDPGQS
                     AEDVAIKRRAFGLDRPVYVRYVKWLGELARGNLGYSYNTGAPVTKRVGERIVPTLKLT
                     GAALLLSYVIGIPVGVVAAAKRYSAVDYLATMLTFLGISLPTFFLGLAGIYIFALRLQ
                     LLPVSGTMTLGGEGGFLDGLHHLVMPASVLAVAGAGLVTRYVRSSMLEVMGQDYVRTA
                     RAKGLAEVLVMRRHALRNALIPVITLAGLQIPALLAGAVITEQVFEWPGMGRLTIEAI
                     SQRDYPVLMGITLIAAVLVAIGNLLADMAYAVADPRIHYE"
     gene            1609..2514
                     /gene="opp4C"
                     /locus_tag="AC159B_01840"
     CDS             1609..2514
                     /gene="opp4C"
                     /locus_tag="AC159B_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019536363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HIR3376527.1"
                     /translation="MSNVASVSRALDGARPTRPVSFWGLATQRFLRHRLAVSGMLVMI
                     VIAAIATGAVWFAPHSPTSIDPVTFQAAPSATHPLGTDSVGRDVLSRLIFAARVSITV
                     GVLAVAMYVVIGTAVGAAAGYYGGKTDLVLSRIMDVMLSFPPLIIILFAVSVFGRPSL
                     WNVIIVLGLLGWPALARLVRGQFLALRDLEYVLAARANGALDARIVLRHMLPNALAPV
                     LVAATFGMANAILVEAALSFLGLGVQPPTPSWGNMLTDAQSLTVLERMPWLWVPPGFM
                     ILISVLSINFLGDGLRDALYPSLRL"
     gene            2546..4162
                     /locus_tag="AC159B_01845"
     CDS             2546..4162
                     /locus_tag="AC159B_01845"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3376528.1"
                     /translation="MKGMRVWLAAIVTLVVAALMIPAGALGGPKSSLVVVSGMWSPPN
                     NFSPINTDSSYGYYCVRFMFQGMLEARLDKNEMKFTPALASSWDVGADRQTFRFTIHP
                     RAAWHDGRPVTSDDVEFTILTISDTRTATNRGAYIARIAGLTAGGKRVPDQQLGFRRL
                     GPKTFELRTKGPVDPQMFLESFGNSVYVLPKHILGDVPPDQLARHPFFLNPTVGNGPY
                     KFVQYKTDQFVEFVANENYHNGAPNVRRVFVRIIPPTTMLAQLERHDMDITAGVGIGE
                     AIAADWDRVKAIPHLRTVSIRAPAYQFMLFNFSRPYLEDKRVRRAMAHAINRPLIVNQ
                     LLKGEGEIAEGPIPPTTPYYNKNVRPWPFDPARARALLQEAGWDFNRTITLRVPTGNI
                     DRERSADIIRENLVAVGVKVEIQKSDFPTHLAALTAKNYDIGLIGWGGLADPDVSSQF
                     RTGGQYNFTHQSLPRMDQLLDEGASAADPARRRAIYNQFQEMFADELPYVVLYYIYGK
                     TAIAKRMSDVLDDVSGLYDFRTYTWIAAPQ"
BASE COUNT          790 a         1288 c         1314 g          852 t
ORIGIN      
        1 tcagcaacat cggggtgtca acgttctata tgctgacgtc gaccatgacc aacgagctcg
       61 tgaaagagaa aggatactat cccgtgggcg gctgcggggc caacattgcc tggcacaccg
      121 aggacgatac cctcgagatc gccgaccgcg accaccttct gcgggacatt cgtgtatacg
      181 cggcggccgc gtttcgtgcg gccaacagtc cgatctaccc gctcgatttc gcggccactg
      241 ccgcggagtt catccgcaca ctggaccggt accaggaggc cgcgggggag gcgttcgatc
      301 ttcagccggt gatcgaagag gcgcggggac tcgagaacag cctggctcgg ttctacgatt
      361 cggcccacca gagcagcccg cgcagcgtga acgatcccga tgtccgccgc atcaacgaag
      421 cacagcgccg tttggcgagg gtccttgtgc cgatcaacta ctcacgggcg gggcgattcc
      481 gccacgaccc ggcgatcgac gtgccgccgc ttccggatct cgagccggcg acgaagatcg
      541 cttccctggc acaggagagc gacgcctatc gattaacgcg cacgcatctg gcacgtggtc
      601 gcaaccgggt ggtcgccgcg ctgcgggaag caaggcgcat catcgaagat gcacaggcct
      661 agcacgtgca ccgatacata gtacggaggc tgttgatcat ggtgcctatc ctgctcggca
      721 tcaccgtgct cagctacctg attctgtcac tcacacccgg ggaccctgtg cagatgctga
      781 ttgatcccgg gcagagcgcc gaagacgttg ccatcaagcg ccgcgcgttc gggctggacc
      841 ggcccgtata tgtgcggtac gtgaagtggc tcggtgagct cgcccgggga aacctgggtt
      901 actcgtacaa cacgggggct ccggtcacca aacgggtcgg cgagcgaatc gtccccacgc
      961 tgaagttgac gggggctgcc ctgctcttgt cctatgtgat cgggataccc gtaggagtgg
     1021 tcgcagcggc gaagcgctac agcgcagtcg attatctggc taccatgctg acgtttctgg
     1081 gcatcagcct gcccacgttc ttcctgggcc tggcgggcat ctatatcttc gcgctccggc
     1141 tgcagttgct tcccgtcagc ggcaccatga cgttgggagg cgagggaggt tttctcgacg
     1201 ggttacacca cctggtgatg cccgccagcg tgctggctgt ggcgggagca gggcttgtca
     1261 cgcgatacgt gcgttcgagc atgcttgaag tcatgggaca ggattacgtg cgcacggcgc
     1321 gagcaaaggg gttggccgaa gtcctcgtca tgcggcgcca cgcgctgcgc aacgcgctca
     1381 tcccagtcat cacgcttgcg gggttgcaga ttcccgcgct gctcgccggc gccgtgatca
     1441 cggagcaggt gtttgagtgg ccgggaatgg gccgcctcac gatcgaggcg atcagccagc
     1501 gtgattaccc ggtcttaatg gggatcacgc tgatcgccgc ggtgctggtg gcgatcggga
     1561 atctccttgc cgacatggca tatgcggtcg cggatccgcg cattcactat gagtaacgtg
     1621 gcgtctgtct cacgcgcgct ggacggggcg aggccgacac gtccggtgtc attttggggt
     1681 ctcgcgaccc aacggttcct ccgtcaccgg ctcgccgtga gcggcatgct cgtcatgatt
     1741 gtgatcgctg cgatcgccac gggtgcggtc tggttcgcgc ctcattcacc aacgtccatc
     1801 gatcccgtga cgtttcaggc cgcgccgtcg gcaacgcatc cgctgggcac cgacagcgtg
     1861 gggcgcgatg tcctgagccg cctcatcttt gccgcgcgcg tgtcgatcac cgtgggcgtg
     1921 cttgcggtcg cgatgtatgt ggtgatcggc accgcggtcg gcgccgcggc gggctattat
     1981 ggcgggaaga cggacctcgt gctgagccgt atcatggacg taatgctgtc ctttccgccg
     2041 ctgatcatca tcctgtttgc ggtgagcgtc ttcggccggc cgagcctatg gaacgtcatt
     2101 atcgtgctcg ggctgctggg ctggcccgcg ctcgcgcgcc tggtccgcgg ccagttcctc
     2161 gcgctgcgcg atctggagta cgtgcttgcc gcgcgcgcca acggggcgtt ggacgcccgc
     2221 attgtgctcc gtcacatgct gcccaatgcc ctcgcgcccg tgctcgtggc ggcgacgttc
     2281 ggtatggcga acgccatcct cgtcgaggcc gctctgtcat tcctcgggct gggcgtccag
     2341 ccgccgactc cgtcatgggg gaatatgctc accgatgccc aatcactcac agtgctggag
     2401 cgcatgccgt ggttgtgggt gccaccgggg ttcatgattt tgatctcggt gctgtccatc
     2461 aattttcttg gagatggatt acgcgacgcg ctttacccca gcttgcgtct ttaaggatgt
     2521 acggcccttg aaagggggag agtagatgaa aggaatgcgt gtgtggctgg cggcgattgt
     2581 cactctggtc gttgccgctc tgatgatacc ggctggcgcc ctcggtgggc cgaagtcatc
     2641 gctggtcgtg gtctccggga tgtggagccc tccgaacaac ttcagcccga ttaacaccga
     2701 tagctcctac ggctactact gcgtgcgatt catgttccag ggaatgctcg aagcgcgcct
     2761 ggacaaaaac gagatgaagt tcacgcctgc gctggccagt agttgggacg tgggagccga
     2821 caggcagaca tttcgattca ccattcaccc gcgcgctgcg tggcacgacg ggagacctgt
     2881 gacgtccgac gatgtcgagt tcacgattct gaccatcagt gatacgagga ccgccaccaa
     2941 caggggagca tacatcgcta ggattgcagg cctgacggcc ggcggcaagc gcgtgccgga
     3001 tcaacagttg ggctttcgca ggctcgggcc caaaacgttc gagctcagaa cgaagggccc
     3061 tgttgacccg cagatgttct tggagtcatt cgggaacagc gtctatgttc tgcccaaaca
     3121 cattcttggc gacgtaccac ccgatcagtt ggcgcgtcat ccattcttcc ttaaccccac
     3181 ggtcgggaac ggcccgtaca agttcgtcca gtacaagacc gaccaattcg tggaatttgt
     3241 cgctaacgag aactatcata acggtgcgcc aaacgtccgg cgcgtgtttg tacgcatcat
     3301 cccaccgacg acaatgctgg cccagttgga acgtcacgac atggacataa ccgcaggggt
     3361 cgggattggt gaagcgattg ccgcagactg ggaccgcgtc aaggccattc ctcacctacg
     3421 aacggtcagc atacgcgcac ccgcatatca gtttatgctc ttcaactttt cgcgtccgta
     3481 ccttgaggac aaacgcgtcc gtcgagcgat ggcccacgcc atcaaccgac cgctgatcgt
     3541 taaccagctc ctcaagggcg agggcgaaat tgctgaagga ccgatcccac caacaactcc
     3601 gtattacaac aaaaatgtca gaccgtggcc gtttgacccg gcgcgagcgc gggcattgct
     3661 gcaggaagcg gggtgggact tcaaccgcac gattaccctc cgtgttccaa cgggcaacat
     3721 cgatagggag aggtctgcag acatcatccg cgagaatctc gtcgccgtcg gggtcaaagt
     3781 ggagatccag aaatccgact tcccgaccca cctggccgca ctgacggcga agaattatga
     3841 tatcgggctc atcggctggg gaggtctggc agacccggac gtaagctcgc agttccgcac
     3901 gggcggccag tataatttca cacatcagtc ccttccgcgc atggatcaac tgctcgatga
     3961 gggtgccagt gcagcggatc ctgccaggcg cagggcgatc tacaaccagt ttcaagagat
     4021 gttcgccgac gagcttccct atgttgtgct gtattacata tacggtaaga cggcaattgc
     4081 aaagcggatg agtgacgtgc tagatgacgt atcaggcctg tacgactttc ggacctatac
     4141 ctggatcgcg gcaccgcagt aaccctgtca tccaccggac gggggtggac ccacatccac
     4201 ccccgtttgg tccttcctcg ctgagataat ccgatgaaag ccgt
//
LOCUS       DBNGSB010000066         5598 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_1270886, whole genome shotgun sequence.
ACCESSION   DBNGSB010000066 DBNGSB010000000
VERSION     DBNGSB010000066.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 5598)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5598)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5598
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_1270886"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="AC159B_01850"
     CDS             <1..731
                     /locus_tag="AC159B_01850"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HIR3376529.1"
                     /translation="NSRVDIALFEAALRDDLNYRAPRILAVLRPLKVVLTNFPDGKVE
                     HLEAPYWPHDVPNKGSRRVPFAKTLFIERHDFMEDPPKGFYRLTPGGRVRLRHAYVIR
                     CEEVMKDAAGEIIELRCTYDPTTLGHPPADGPVKGTIHWVSAAHAVQAEMNLYDRLFM
                     VADPEATETEFTDRLNRESLTVARGYVEPSVAQDARDLRYQFERLGYFWRDPVDSRPE
                     RLTFNRIVSLRDSWNRRSRAQRPS"
     gene            complement(765..2246)
                     /gene="gltX"
                     /locus_tag="AC159B_01855"
     CDS             complement(765..2246)
                     /gene="gltX"
                     /locus_tag="AC159B_01855"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIR3376530.1"
                     /translation="MSQIRTRTAPSPTGYLHVGNAWSAFFNWLLTRNQGGVFVLRIED
                     TDRSRSTEQFEASILDDFKWLGITWDEGPDAGGPYGPYRQTERMHMYRQYADELIQRG
                     AAFHCFCTREELDAERQSAEMEGRAYRYSGRCRTLADSQRAIFIAEGRRAATRMKIDP
                     GPPIVVNDLVQGRVVFEPDVLDDFIIVRSNESPLYNFANVVDDHLMAMTHIVRGSEHL
                     NNTPRQLVIYKALGWTPPQLAHLPVILGPDRKKLSKRHAATAVSEYRREGYLPEAMLN
                     FFALMAWYPEENREIYSIDELVRKFRIQDVNNASPIFDVTKLTWMNGVYMRDLLGKDP
                     DRVVTMCQDVLRDAGLLDGQLTPEVRTYITRVVEVLGDRIKTGRDILAYGDFFFTDAV
                     TYEPDAVEKYFSRKGSTELLAQLRERVQRADQFDASATEAIVRDMASELGIPTAEIVH
                     PARVAMTGKTAGPGLFELMALLGRDRVVQRLEKAIDWIRISVS"
     gene            complement(2337..3287)
                     /locus_tag="AC159B_01860"
     CDS             complement(2337..3287)
                     /locus_tag="AC159B_01860"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HIR3376531.1"
                     /translation="MDADYGEMKIFCGTANPDLGEEIAAYLSIPRGKVTSTRFADGEI
                     YVRFEENARGVDAFVVQPTSHPVNETLMELLIMLDALRRASAGRITAVMPYYGYARKD
                     KKDAPREPITGRLVADLLISSGANRVLTVDLHAGQIEGFFNIPVDHLRAMPLFADYLR
                     EKDLRNAVVVAADDGAVKRSKQLADRLDLPLAIIFQRRVGADAKEPIEIVGDVEGMIP
                     VMIEDVIDTAGTLSGAVDVLVRKGARPEVYICASHPVFAGPARQRLDRTEVREVIVTN
                     TIPLPPEKMLPKVTVLSAAPLLAEAIRRIHLNQSVSMLFT"
     gene            complement(3379..3642)
                     /locus_tag="AC159B_01865"
     CDS             complement(3379..3642)
                     /locus_tag="AC159B_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HIR3376532.1"
                     /translation="MPEVLKVSADSKPKAVAGALAAVLREKGKVEMQAVGAGAVNQAV
                     KAIAITRGFVAPNGIDLVAIPAFTKVIIEGEERTAIRFLVEAR"
     gene            complement(3717..4841)
                     /gene="csaB"
                     /locus_tag="AC159B_01870"
     CDS             complement(3717..4841)
                     /gene="csaB"
                     /locus_tag="AC159B_01870"
                     /inference="COORDINATES: protein motif:HMM:TIGR03609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046919 - pyruvyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase CsaB"
                     /protein_id="HIR3376533.1"
                     /translation="MGRAGDRDRGDLPAPPLVTSIVISGYYGFANVGDEAVLASMLNS
                     LRNRIPDARFVVLSADPAGTTRLHGVPSISRVGPGVLGAITGADLFISGGGSLIQDAT
                     SARSALYYVGVLAYAARAARRSMVFAQGLGPIRRRWIRALVRRTLDRVHVISFRDEDS
                     QRLAHEIGVRRPSQLVADPVFALDPAPFERAAHLLEGLPRPRFGLVLRPVAGIQYVQH
                     VVDAVRSTVQHTGGSVVALVFHRRYDLAITRHIAEALQSRVISDLAPSEMMAVVGALD
                     VLAGVRLHALIAAVAAGVAPVGLSYDPKVESLFRKVGAGYLMPVAGLQPEPLKQALLA
                     AWEGGEALRPQLLAAAARLREEALRATDLAASLLSLPAPF"
     gene            complement(4787..5437)
                     /locus_tag="AC159B_01875"
     CDS             complement(4787..5437)
                     /locus_tag="AC159B_01875"
                     /inference="COORDINATES: protein motif:HMM:NF039831.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5693 family protein"
                     /protein_id="HIR3376534.1"
                     /translation="MQTLWVASAVSIGGGLLVAALLTSWDFMMASQVFLGVKVAHLIP
                     VVLAAVLIGSSSRTPRSWRETAQALWEWSAHPLRMRYAMVAVLIGFAGIVLLARSGNF
                     GLPVLAIEDRLRTITEDVLVARPRTKEYLIGHPALFLAGAAMAAGWRRWVLPLAAIGA
                     VGQAGIINSFSHIHTPLVYTLLRTINALVLGTVFGVIGTWVVLAIATAATSPRRPS"
BASE COUNT         1132 a         1772 c         1681 g         1013 t
ORIGIN      
        1 caaacagccg cgtggatatc gcgctctttg aagcggccct tcgcgacgac ctgaactacc
       61 gtgccccgcg catcctggca gtcctcaggc cgctcaaggt cgtcttgacg aacttccccg
      121 atgggaaggt cgagcacctg gaggcgccct actggccgca cgatgttccg aacaaagggt
      181 ccagacgcgt accgttcgcg aagacgctct tcatcgagcg ccacgatttc atggaagacc
      241 cccccaaggg gttctaccgt ctaactcctg gaggccgagt gcggctgcgc cacgcgtacg
      301 tgatccggtg cgaagaggtg atgaaggatg cggccggaga gatcatcgag ttacggtgca
      361 cctacgatcc caccaccctc gggcatcctc cggccgatgg accggtcaag ggcacgatcc
      421 attgggtttc cgcggcgcat gccgtccagg ccgagatgaa cctgtacgac cgtctgttta
      481 tggtcgcaga tcccgaagcc accgaaacag aatttactga tcggctgaat cgggaatcct
      541 tgaccgtcgc gcgtgggtac gtcgagccaa gcgtggcgca ggacgcgagg gatcttcgct
      601 atcagttcga gcgcctgggg tacttctggc gtgaccccgt cgattcgagg ccggagcgct
      661 tgaccttcaa ccgcattgtg agcctgcgtg attcctggaa caggcgaagc cgcgcacaga
      721 ggccgagcta gaccgaggcc gggggtatct tgcacgttgc cgggttaaga gaccgatatc
      781 cggatccagt caatcgcctt ttctaaccgt tggacaacgc ggtcacgacc aagcaacgcc
      841 atcaactcga acaaccccgg acccgccgtc ttgccggtca tcgccacccg ggcgggatgc
      901 acgatctccg cggtcggaat cccaagctcg ctcgccatat cgcgaacgat ggcttccgtt
      961 gccgaggcat caaactgatc agctcgctgg acacgctcac gcagctgcgc gagtagttcg
     1021 gtgcttccct tccggctgaa gtacttctct acagcgtctg gctcatacgt gacggcatcg
     1081 gtgaagaaga aatcgccgta cgcgaggatg tcgcggccgg tcttgattcg atccccaagc
     1141 acttcgacaa cacgcgtgat gtacgtacgt acctctgggg tcagctgacc gtccagcaag
     1201 ccggcgtctc tcaacacatc ctggcacatc gtcaccaccc ggtctggatc cttgccaagc
     1261 agatcccgca tgtacacgcc attcatccac gtgagtttgg tgacgtcgaa aatgggggac
     1321 gcgttgttca cgtcctgaat acggaacttc cggacgagtt cgtcgatcga atagatttca
     1381 cggttctctt ctgggtacca cgccatcagc gcaaagaagt tcaacatcgc ctccggcagg
     1441 tacccctctc ggcggtactc gctgaccgcc gtggcggcgt gacgtttgct gagcttcttg
     1501 cgatcgggcc cgagaatgac gggcaggtgg gcgagctgcg gcggcgtcca gcccagcgcc
     1561 ttatagataa cgagctgccg cggcgtgttg ttgaggtgtt cgctgccgcg cacgatgtgc
     1621 gtcatggcca tgaggtgatc gtccacaacg ttcgcaaagt tgtacaaagg cgactcgttg
     1681 gacctcacga tgatgaaatc atccagaaca tccggctcga atacgacgcg cccctgcacg
     1741 agatcattca cgacgatggg tggaccgggg tcaatcttca ttcgagtcgc cgcccggcgt
     1801 ccttcggcga tgaatattgc gcgctgagaa tctgccaggg ttcgacagcg gcccgagtac
     1861 cgataggcgc ggccctccat ttccgccgac tgtcgttctg cgtcgagctc ctcgcgcgtg
     1921 cagaaacagt gaaacgcggc accacgctgg atcagctcat ccgcgtactg ccggtacata
     1981 tgcatccgtt ccgtctgacg gtacgggccg tacggcccgc cggcgtccgg cccttcgtcc
     2041 caggtaatcc cgagccattt gaaatcgtcc agaatcgatg cttcaaactg ctcggtggaa
     2101 cgcgatctgt ccgtgtcttc gatgcgcagg acgaagacgc cgccttgatt gcgtgtgagc
     2161 agccagttga aaaaggcact ccaggcattg ccgacgtgga ggtacccggt gggacttgga
     2221 gctgtgcggg ttcggatctg ggacataagt caaggaaaaa ggaaaaagga aaatggaata
     2281 aggacaagtc tcagagttaa tccccttttc cctttacctt atacctttga cctttgtcag
     2341 gtgaagagca tactcaccga ttgattgagg tgaatccgcc ggatggcttc ggccagcaac
     2401 ggagcagctg agagcaccgt gactttcggc aacatcttct ccggcggcag cgggatggtg
     2461 ttggtgacga tgacctcgcg gacttccgtc cggtccagcc gctgccgggc cggaccggcg
     2521 aacacggggt gcgacgcgca gatatacact tccggtcgcg cgcccttccg cacgagcacg
     2581 tcgacggcac ccgacagcgt gcccgccgtg tcgatcacat cctcgatcat gaccgggatc
     2641 atgccttcga cgtcgccgac gatctcgatc ggctccttcg cgtccgcgcc tacgcggcgc
     2701 tgaaagatga tcgcgagtgg caggtcgagg cgatcggcca gctgcttgct gcgtttcacc
     2761 gcgccgtcgt cggccgcaac gaccacggcg ttgcggagat ccttctcgcg cagatagtcg
     2821 gcgaacaggg gcatggcgcg gaggtgatcg acaggaatat tgaagaaacc ttcgatctgc
     2881 ccggcgtgca gatccacagt cagcacccgg ttggcgcctg aactgatcag caggtccgct
     2941 acgagacgtc ccgtgatcgg ctcgcgcggg gcgtccttct tgtcctttcg cgcatatccg
     3001 taatagggca tcaccgccgt aatgcggccg gcggatgcgc ggcgtaacgc atcaagcatg
     3061 atcagcagtt ccatcaaggt ctcgttcaca ggatgactgg taggctggac gacaaaggcg
     3121 tcgaccccgc gggcattttc ctcgaagcgc acgtaaatct cgccgtcagc gaagcgcgtg
     3181 ctcgtgactt tgcccctggg aattgaaagg taggcggcta tctcctctcc caggtcggga
     3241 ttagcagtgc cgcagaagat cttcatctcg ccgtagtcgg cgtccatctt cacaatggta
     3301 aaaggaaaaa ggtaaaagga aaaggcgcaa aacaagcgcc atcccttttc ccttttccgt
     3361 tttccttatt cctttgtctc agcgggcttc tacgagaaac ctgatggcag tacgttcttc
     3421 gccctcgata atgaccttcg taaatgcggg gatggccacg aggtcaattc cgttgggggc
     3481 aacgaacccg cgcgtgatcg cgatcgcctt caccgcctga ttgaccgcgc cggcgccgac
     3541 agcctgcatc tcaaccttac ctttttccct tagcaccgcg gcaagtgctc cggcgactgc
     3601 cttaggcttg gagtcggcag agaccttgag cacctcgggc attgcagccc tcgatgctgc
     3661 gacgaatggt ccatccattg gctttccact acttcgtaga ggtgcaaaga aatcctctag
     3721 aatggcgccg gcagtgatag caatgacgcc gccagatcgg ttgcgcgcag ggcttcttca
     3781 cgaagccggg cggccgcggc caggagctgc gggcgaagcg cctccccccc ttcccaggcg
     3841 gcgagcaggg cctgcttcag cggttccggt tgcagtccgg cgaccggcat cagatagccg
     3901 gctccaacct ttcggaagag actctctact tttgggtcgt atgagaggcc gaccggcgcg
     3961 actcccgcag ccacagcggc aatcagggca tgcagccgga cgccggcgag cacgtctaac
     4021 gcgccgacca ccgccatcat ttctgagggc gcaaggtcag agatcaccct cgactgcagg
     4081 gcttccgcga tatgccgcgt gatcgcgagg tcgtaccgcc ggtggaacac caaggcgacc
     4141 acggagccgc cggtgtgctg gacggtcgac ctgacggcat cgacgacgtg ttggacgtat
     4201 tggatcccag caacaggacg gaggaccaga ccgaaccttg ggcggggcag accttcaagc
     4261 aggtgcgcag cgcgctcaaa aggcgccggg tcgagggcga acacaggatc cgcaaccagc
     4321 tggctgggac ggcgcacccc gatctcatga gccaaccgct gggagtcctc atcgcggaac
     4381 gagatgacat gaacgcgatc gagcgtccgc cggacgagcg cacgaatcca ccgtcgccgg
     4441 atcggcccga ggccttgcgc gaacaccatc gaccggcgtg cggcacgcgc ggcataggcg
     4501 agcacaccga cgtagtagag tgcgctgcgc gcgctggtgg catcctgaat caggctcccc
     4561 ccgccgctga tgaagagatc ggcaccggta attgccccta gtactcctgg gcccacgcgc
     4621 gagatgctcg gcacgccgtg cagccgtgtt gtcccggccg gatcggccga cagcacgacg
     4681 aaccgcgcat caggaattcg gttgcgcagg ctgttgagca tcgacgcaag taccgcctcg
     4741 tccccgacgt tcgcaaaccc gtaatatcca gagatgacaa ttgaggtcac gaggggcggc
     4801 gcggggaggt cgccgcggtc gcgatcgcca gcacgaccca agtgccgatg actccgaaga
     4861 ccgtgccgag gaccagcgcg ttgatggtgc gcaacagcgt gtacaccaac ggcgtgtgaa
     4921 tgtgcgagaa gctattgatg atccccgcct gtccaacggc gccgatcgcg gcgagcggca
     4981 acacccagcg ccgccatccg gccgccatcg cagcgccggc aaggaacagc gctgggtggc
     5041 caatcaagta ctctttcgtc ctgggccgcg cgacaaggac gtcttccgtg atcgtacgca
     5101 accgatcctc aatcgccagg acgggcaggc cgaaatttcc gctgcgcgcc agcagcacaa
     5161 tcccggcgaa gccaatgagg accgccacca tcgcgtaccg catccgcagc gggtgtgccg
     5221 accattccca taaggcctgc gcggtttccc gccaggagcg tggtgtgcgg gacgagctcc
     5281 ctatcaacac cgcggcgagc acgaccggaa tcaggtgggc gactttcact ccaaggaaga
     5341 cctgcgaggc catcatgaag tcccatgacg tgagcaacgc cgcgacgagc aagccacccc
     5401 cgattgatac agccgacgcc acccagagtg tttgcacgcc cgcccacagc gcgccgagcc
     5461 ttgactcgct cggaaaaatc cgccgcagtt aaaacacgac gcccagcgcc gcggtgaccg
     5521 actcggttcc cagagacagc agattgctcc agagcactat cggcccggca agaagcagcg
     5581 cggccgtcag cacaattc
//
LOCUS       DBNGSB010000067         2925 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3418721, whole genome shotgun sequence.
ACCESSION   DBNGSB010000067 DBNGSB010000000
VERSION     DBNGSB010000067.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3418721"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            334..1092
                     /locus_tag="AC159B_01880"
     CDS             334..1092
                     /locus_tag="AC159B_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376535.1"
                     /translation="MRSDMAKVVTERPRRGHGNPSKKWGRRLRKHEYDAEDHGPTRAA
                     IARRHQYGWNAKEFSDLLGPLRRYLHKQVGRPWDKVWSEITRNLDSRSLSGQHIFDHI
                     QWEVEQHAWLGPDGTLYRNRWSGLLPVSGLYVHPTTRLLCYTRESWRGYRGGPFLKAQ
                     AALRVFGVDASKAADIRRYRVDGTRLWERRDCGWFIHTYRYVPEQLVRVIIRSDGHEV
                     PIYSPPCYERVATKQASKREIREARLILERDPSA"
     gene            1132..2367
                     /gene="serS"
                     /locus_tag="AC159B_01885"
     CDS             1132..2367
                     /gene="serS"
                     /locus_tag="AC159B_01885"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HIR3376536.1"
                     /translation="MIDIRELRRNPEAYLAKLARKGAGELGQELLDLDAAWRATTTAA
                     ESLRAKLKSSGKPTAEQREELQRAKEQFQETESKLAELEARRRALLDRVPNPPADDVP
                     SGGEEDFEVLREMGTKPSFAFTARDHLDLAQPHGWLDAPRGTKASGSRFVYRLGDLAL
                     LEMALYRYAFTRVTEQGHIPMLPPVLVREEAMYGTGFFPTDAVNIYGVERDQLYLVGT
                     SEVPAAAFHAGEFLAELPLRYVAYSTCFRREAGAAGKDTRGMFRVHQFDKVEMFVYCL
                     PEASADEHERLLAIEEGLVKELGLPYRVINVAAGDLGAPAVKKYDIEAWFPSQGRYRE
                     ITSTSNTTDFQARRLDIRFRRGDKPEHVHTLNGTAITARAMIAILENFQEADGSIGVP
                     RVLWEFGAPRRMGSGRAGA"
     gene            complement(2686..>2925)
                     /locus_tag="AC159B_01890"
     CDS             complement(2686..>2925)
                     /locus_tag="AC159B_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376537.1"
                     /translation="HLLRRTYVRRQTVDERLEPVGPQRVMDLRPITRLRRLGAAGSEQ
                     SMARTLTDAITRPIQRATPGCDESGPAVFSRNERS"
BASE COUNT          599 a          890 c          935 g          501 t
ORIGIN      
        1 gtagagacca gttctatgga cgttaagtca attaaagtat attattatgg acgttatgtc
       61 aacttgtcgg ccggccctaa gagagcaccg gcgacaaagc attgaggcgc ggagggcaca
      121 atcagcaatg accaatgcca gcaatgctgc agcgtcaggt cgatagggat ccctgacctg
      181 ttgaacatca acgtcccaat gacaccagcc ggctgggaat gcgctcgaac cggaaatgcg
      241 ctatcctgtc cggctctcgc ggcgaggatt gaccgaatga ctccgacccg attcttcaga
      301 ggaggtgacc tatgacgtag tggaggaaca cgtatgcgaa gcgacatggc caaggtcgtt
      361 accgaacggc cacgacgagg ccacggaaat ccgagcaaga agtgggggcg acgactccga
      421 aagcacgagt acgacgcgga ggatcacgga ccgacgcgcg cagcgatcgc gcggcgtcat
      481 caatacggct ggaacgcaaa ggagttctcc gacctcctgg gaccgctccg gcggtacctg
      541 cataagcaag tcggacgccc ttgggacaag gtctggtcgg agatcacacg caatctcgac
      601 agccgttcac tgagcggcca gcacatcttc gaccacatcc agtgggaggt ggagcagcac
      661 gcctggctcg gtccagacgg cactctgtac cgcaatcgtt ggtcagggct gctcccggtc
      721 agcggcttgt acgttcaccc gactacgaga ctgctttgct acacccggga gtcctggcgc
      781 gggtaccgcg gtggcccgtt cttgaaagct caggctgccc ttcgcgtgtt cggtgtcgac
      841 gcatcgaagg cagcggacat tcgacgctac cgcgtagacg gcacgcgact gtgggagcgt
      901 cgcgactgcg gctggttcat tcacacctac cgctacgtgc ccgaacaact cgttcgagtc
      961 atcatccgca gtgatggcca cgaggtcccg atctacagcc cgccctgcta cgaacgcgta
     1021 gccacgaagc aggcgagcaa acgggaaatt cgggaggcgc gactaatctt ggaacgcgac
     1081 ccgagcgcct gagagtgggc gcgcggatgt cacaaaggac caaccgctgc aatgatcgat
     1141 atccgtgagc tgcgtagaaa tcccgaggca tacctggcca aactggcccg caagggcgca
     1201 ggcgaactcg gtcaggaact gctcgacttg gacgcggcct ggcgcgcgac aacgacagcg
     1261 gcggagtcgc tgcgcgcgaa actgaagtct tccgggaagc cgaccgcgga gcaacgggag
     1321 gaactgcagc gggcgaaaga gcagtttcag gagaccgagt cgaagcttgc cgaattggag
     1381 gctcgtcgta gggcccttct cgatcgcgtg cccaatccgc cggctgacga cgtgccgagt
     1441 ggcggagaag aggatttcga ggtgctccgc gagatgggca cgaagccgtc gttcgccttc
     1501 acagctcggg accatctcga tctcgcccag cctcacggat ggctcgatgc gccgcgcggt
     1561 acgaaggcct ccggatcgcg gtttgtctat cgcctgggcg atttggcgtt gctggagatg
     1621 gcgctctacc ggtatgcgtt cacgcgagtg accgagcagg gtcacatccc gatgctcccg
     1681 cccgtgctcg tgcgcgagga agcgatgtat ggcaccggtt tctttcctac agacgccgtc
     1741 aacatctacg gcgtcgagag ggaccagctc tatctggttg gtacgtccga ggtgccggcc
     1801 gctgcgtttc atgcgggaga gttcctcgcg gaattaccgc tccggtacgt ggcgtactcc
     1861 acctgcttca ggcgtgaggc cggcgccgcg ggcaaggaca ctcgcggcat gttccgcgtg
     1921 caccagttcg acaaggtgga gatgttcgtg tactgcctgc cggaggcctc agccgacgag
     1981 cacgaaaggt tgctcgcgat tgaggaaggg ctcgtcaagg aactcggcct gccctaccgc
     2041 gtgatcaacg tcgcggctgg cgatctcgga gcgccggcgg tcaagaaata tgacatcgaa
     2101 gcctggttcc ccagccaggg tcgatatcgc gagatcacgt cgacctcgaa cacaactgat
     2161 tttcaagcac gtcgcctcga catccgcttc cgccgcggtg ataagccaga gcatgtgcac
     2221 acgctcaatg gcacggcgat caccgcgcgg gcgatgattg cgatcctgga gaattttcag
     2281 gaggcggacg ggtcaatcgg cgtcccacga gtgctgtggg agtttggcgc tccgcgcagg
     2341 atggggtcgg gtcgcgctgg ggcttgacac gcgaaaccga cgcgcgtatt ttcaaatcat
     2401 ctcgcgtggc gatcgatcgc caccctccac gatgggccac aggccacgtg gaattcgctt
     2461 ccgcccagtc gccctcaggg cacgggaaga agcgcccgat agccgggtcg gtgcagagag
     2521 tcgcagctag gcagcccgag gacaccccag ggggctcgag gcaaccagag acagcgcgac
     2581 tcgccacgat cagcggttcg gtcatcatga ccagccgcat cgatgagcac caccgagggc
     2641 cggcacaccc ctaaaacaga aggcgtttcc gcgtacgacg gctcttcagg accgctcgtt
     2701 ccgcgagaac accgcggggc cggactcgtc gcaccctggt gtggcgcgtt ggatgggcct
     2761 cgttatggcg tcagtcagag tgcgagccat gctctgctct gaacccgccg caccgagccg
     2821 ccgcagacgc gtgatcggcc tcaggtccat cacgcgctgc ggacctaccg gctcgagacg
     2881 ttcatcaaca gtctggcgtc gaacgtacgt tcggcgcagc aagtg
//
LOCUS       DBNGSB010000068         5918 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2498884, whole genome shotgun sequence.
ACCESSION   DBNGSB010000068 DBNGSB010000000
VERSION     DBNGSB010000068.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 5918)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5918)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5918
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2498884"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="AC159B_01895"
     CDS             <1..803
                     /locus_tag="AC159B_01895"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019342.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HIR3376538.1"
                     /translation="GRRRVFTDRWERAMRVLQWRRRPNRIAVRGGAPRVGITVNDPST
                     QSARRYASAVEAAGGVPRWITPADLRAAGERAALRNIDALLLSGGRDIDPRMYAESLT
                     PGLDVDVDANRYEVEISLGRAVLEERMPVLGICGGMQVLNVASGGSLYQDISVLGIDP
                     AVHRVIGRDVFHDIRIVPGSRLAEVLGLRELKVNSAHHQAVKQPGRGAVITAAAPDGV
                     IEAIEFPGTSFAIGVEWHPERMPDDDHQRLLFHALIAAARTAQPRSRA"
     gene            800..2581
                     /locus_tag="AC159B_01900"
     CDS             800..2581
                     /locus_tag="AC159B_01900"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF014665.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HIR3376539.1"
                     /translation="MKSFARLVLGTTAATYFLIVLGGVVRATGSGLACPDWPLCHGRL
                     IPPLEGPILIEYSHRLVASVVGLLTGAVALQTWRLRDARPLRGLSILALLLVGVQILL
                     GGLTVRTELSEWIVVAHLGTAMAFFATLTILSARAMIGTGKSVSSGDTRGFRRLVVLT
                     ACATYLLVLIGGYVSASGAGLACPDWPLCTGELLPPLSGAVGIHVLHRLAAAFVSVLI
                     VATTIAAYRIHRQPLLQAASAIATGLLVLQIILGALNIEYRLAPGATTAHLANAAALF
                     ATLIALAVLAFKVPEPLAPLPKPLLPEGRGVARILDYVSLTKPRIIVLLLVTTGAAML
                     VAAPGVVSPWVILSTLIGGALASGSAGVINQILERDIDALMARTRRRAVAAGRVDPPS
                     AFAFAVILGALAFAILASLVNLLSAILAMLGLLFYVFIYTVWLKRATPQNIVIGGAAG
                     AVPPLVGWAAATGRVELPAMLLFLIVFLWTPPHFWALSLYRLDDYARAHVPMLPVVAG
                     ENETRRQIVIYSMLLVATTLVLYPVGSMGWMYASAAAALGGVFLWRAIHVRRAQTPQS
                     ARGLFSYSIVYLALLFAAMVMDRMLLT"
     gene            complement(2540..3712)
                     /locus_tag="AC159B_01905"
     CDS             complement(2540..3712)
                     /locus_tag="AC159B_01905"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3376540.1"
                     /translation="MRIHALGLMGFGAITSIMMTIPILSLYLNDRGLSPAHVGAVIGV
                     MSLALVVTEVLAAPAASSRLGRRATVAIALAGSAVMLTVFPQVRSLTGFYLNRLIFGG
                     LRGLLWPVMFAEVAEQAPARREASFAVFWLYFGLGTLVGPALGGILGDRFSLTAPFFV
                     AAAISVLTLSLVIAVRPFRDPGIGNPLAAYRRLLRISPAMPRAWALTLCNVTIFSVYS
                     TFLPLHAASRGLSAAQIGLIFTGGAVSFILGQDLLRRFVDRIPAERLLGPAFVVRGLG
                     IAIVPLLHSFESLLVANFLSALVGAAVPLALTTRVAALAPPDQLVPAMGGFNAAADMG
                     FFVGPVVGGLLAVFGLGWTFGAVLPVLAISVLLLRDQHRIPRRLGEQHPIHDHGGE"
     gene            3818..4024
                     /gene="rpoZ"
                     /locus_tag="AC159B_01910"
     CDS             3818..4024
                     /gene="rpoZ"
                     /locus_tag="AC159B_01910"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HIR3376541.1"
                     /translation="MIKPPLEALLGRVENKYALVIVAAKRARQLKEGALPMVDIDSAN
                     PVTIALEEIAAGKIRFELPKLGLK"
     gene            4112..4576
                     /locus_tag="AC159B_01915"
     CDS             4112..4576
                     /locus_tag="AC159B_01915"
                     /inference="COORDINATES: protein motif:HMM:NF024102.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsuPI-related putative proteinase inhibitor"
                     /protein_id="HIR3376542.1"
                     /translation="MLIGLVLVALVAAAPGAPLMVERADGGLRHEASISKRAFDAGES
                     IEVTITVRNAGSNPVTLTFFSGQRFDLLVRRPRGDEIWRWSHDKAFIQIVQSIPIRPN
                     EPLTLKGSWDQRDYQGRRADPGTYEVVAFVTGRTEGSERSSIQLPALPFTIR"
     gene            4604..4680
                     /locus_tag="AC159B_01920"
     tRNA            4604..4680
                     /locus_tag="AC159B_01920"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4638..4640,aa:Met,seq:cat)
     gene            4891..>5918
                     /gene="tilS"
                     /locus_tag="AC159B_01925"
     CDS             4891..>5918
                     /gene="tilS"
                     /locus_tag="AC159B_01925"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIR3376543.1"
                     /translation="MNDMDLFEKFKATIVDHRLISNGDTVIVAVSGGADSTALLHLLA
                     SLRDQWNLTLVVAHLDHRLRVDSADDARFVLRMAAELGVAPVTDVADVRGLARRRKRS
                     IEEAGREARYEFLERVAREHGARRIATAHTQDDQIETVLMRILRGDPWDAQGGIPIVR
                     PLNAAQVIRPLLMGAREEVRRYLAGRSIEWREDPSNRDVRFDRNWIRLRVLPDLERLT
                     PGVRELLLGFSSAARLADHLLNELAHQHAANVAEAGEHMIRIPLPEFRRHPQDVQRRM
                     LRWAASQITGTTTRGPAGWEKRAHQAATQGRAGDVVDGAIAVRVNYDALEVARPQSQK
                     PSREYRLK"
BASE COUNT         1108 a         1855 c         1877 g         1078 t
ORIGIN      
        1 gaggtcgtcg tcgtgtcttc acagaccgat gggaacgcgc catgcgcgtt ttgcagtggc
       61 ggcgacgtcc caatcgtatc gccgtgagag gcggcgcacc ccgcgttggc atcacggtaa
      121 acgatcccag cacgcagtcg gctcggcgct atgcatcggc cgtggaagcc gccggtggag
      181 taccacgctg gatcacgccg gcggacctcc gcgcggccgg tgaacgcgcc gcgctgcgga
      241 acatcgacgc cctcttgttg agcggcggcc gggatatcga cccgcggatg tatgcggaga
      301 gcctcacccc ggggctcgac gtcgacgttg atgcgaaccg gtacgaagtg gagatctcat
      361 tgggacgtgc tgtgctcgag gagcgtatgc ctgttctagg gatttgcgga gggatgcagg
      421 ttttgaacgt cgcctcgggt ggcagtctct accaggacat ctccgtcctc gggatcgatc
      481 cagccgtgca tcgagtcata gggcgcgacg tgtttcatga catccgcatc gtccccggga
      541 gccgcctggc ggaggtcctt ggactgaggg aactcaaggt gaacagcgcc caccatcagg
      601 ccgttaaaca gccgggccgc ggcgccgtca tcacggcggc cgcacccgat ggggtgattg
      661 aagccattga gttcccgggc acctcgtttg ccatcggggt agagtggcat cccgaacgaa
      721 tgcccgacga tgatcaccaa cgcttgctct tccacgcctt gattgctgcc gcacgaaccg
      781 cccaaccacg ttctcgcgca tgaagtcatt tgcgcgactc gttcttggaa caactgccgc
      841 cacatacttc ctgattgtgc tcggtggtgt ggttcgagca acgggatcgg ggctggcgtg
      901 tcccgactgg ccactctgtc acggacggtt aatcccgccg ctcgaaggac cgatactgat
      961 cgaatacagt caccggctcg tcgcttccgt agtcggattg ctgacaggtg ccgttgcgct
     1021 acaaacgtgg cgcctgcggg atgcgcgtcc cctgcggggt ctctcgatcc ttgcgctgct
     1081 gttggtcggc gtgcagattc tactcggcgg gttgacggta cgaacggagc tcagcgaatg
     1141 gatagtcgtg gcgcacctcg gcacggcgat ggcgttcttc gcgaccctga caattctctc
     1201 cgcgcgtgcg atgatcggaa caggcaaaag tgtgtcgagc ggagacaccc gtgggttccg
     1261 ccggctcgtg gtcctgaccg cgtgtgccac ctacctgctg gtcctgatcg ggggatatgt
     1321 cagcgccagt ggtgcgggcc tggcctgtcc tgactggccg ctgtgcacgg gggaactact
     1381 tccacccctc agcggcgctg tcggcattca cgtcctgcac cggctcgccg ctgcgttcgt
     1441 cagcgtattg atcgtcgcca cgaccatcgc ggcctacagg attcatcgac agcctctact
     1501 gcaggcagcg tcggcgatcg cgaccggtct gttggttctt cagatcatcc tcggcgcatt
     1561 gaacatcgag taccgcctgg cgccgggcgc caccacggcg caccttgcca acgcggcggc
     1621 tctgttcgcc acgctgatcg cactcgccgt ccttgccttc aaggtgcccg agccccttgc
     1681 ccctctaccc aaacccctct tgccagaggg gagaggagtg gctcgaatcc tcgactacgt
     1741 gagtctcacc aaacctcgga tcattgtgct gctcctggtc acgactggag ctgcgatgct
     1801 agtcgccgcg cccggcgtcg tctcgccgtg ggtcatcctg agcacactca tcgggggtgc
     1861 actggcctcg ggatcggccg gcgtgatcaa tcagatactc gagcgagaca tcgatgctct
     1921 tatggcgcgc acccggcggc gagccgtggc cgcaggcagg gtggatccgc cgtccgcgtt
     1981 cgcgtttgcg gtgatcctcg gagccctcgc ctttgcaatc ctggcgtccc tggtgaatct
     2041 cttgagcgcg atactggcga tgctcgggct gctgttctat gtcttcatct ataccgtgtg
     2101 gctgaagcgc gcgacgcccc agaacattgt gatcggtggg gccgccggag cagtgccgcc
     2161 tctggtgggt tgggccgcgg cgacaggccg ggtagaactg cccgcgatgc tgctctttct
     2221 gatcgttttc ctctggacac cgccgcattt ctgggcgctg tcactgtacc gcctcgacga
     2281 ttacgcgcgc gcccatgtac cgatgctgcc ggtggtggct ggagagaacg aaacgcggcg
     2341 ccagattgtg atctattcca tgctgctggt tgcgacaacg ttagtcttat atccggttgg
     2401 ttcgatgggt tggatgtatg catctgccgc ggcagcgctc ggcggtgtgt ttctgtggag
     2461 agccattcac gtgcgacgcg cccagacacc acaatccgcg aggggactct tcagttactc
     2521 gattgtgtac cttgctctcc tattcgccgc catggtcatg gatcggatgt tgctcaccta
     2581 agcgtctcgg gatcctgtgt tgatcccgca ggagcaacac tgagatcgcg agcaccggta
     2641 ggacggctcc gaacgtccat cccaggccga agacggccag taaccctccc acgaccggcc
     2701 cgacaaagaa ccccatgtcc gccgcggcgt tgaagccgcc catcgccggt acgagttgat
     2761 caggcggcgc cagcgccgcg acgcgcgtcg tgagcgccag gggcaccgcc gcgccgacta
     2821 atgcggacag aaagtttgcg accaggagcg attcgaagga gtgcaggagc ggtacgatcg
     2881 cgattccgag cccccgaacc acaaatgccg gtcccagcag ccgctcggcc gggatgcgat
     2941 caacgaatcg acggagcagg tcctgaccca gaatgaagga gaccgcacct cctgtgaaga
     3001 tgagaccaat ctgtgccgcg gaaagtcccc gcgatgccgc gtgcagcggc aagaacgtcg
     3061 agtacacgct gaagatggtg acgttgcaca aggtgagcgc ccaggcacga ggcatcgccg
     3121 gtgagatgcg cagcagcctt cgataggcgg ccaacggatt accgatgccg gggtcgcgga
     3181 acggccgcac ggcgataacc agcgacagcg tcagcacgga gattgccgcc gcgacaaaga
     3241 acggcgctgt cagcgagaac cgatcaccta gtatgccacc cagcgccggg ccgacgagcg
     3301 ttcccagccc gaaatacagc caaaagactg caaacgatgc ttcacgacgc gcaggcgctt
     3361 gttcggcgac ctccgcgaac atgaccggcc acaatagacc gcgcaggccg ccgaagatca
     3421 gccggtttag gtaaaagccg gtcaacgaac gcacttgcgg aaagaccgtg agcatcaccg
     3481 cgctgccggc gagcgcgatt gccacggtcg cacgccggcc caggcgggac gatgcggccg
     3541 gagcggcgag gacctcagta accaccaatg ccagcgacat cacgccgatc accgcgccga
     3601 catgcgcggg tgacaacccc cggtcattca ggtaaagcga gaggataggg atcgtcatca
     3661 tgatcgacgt gatggcgccg aaccccatca aaccaagagc atgaatgcgc acggccccta
     3721 ctctagcacg gctccccgtt gccttgacag cggggcgcgc gttgatacaa tagggtgtta
     3781 ctcgcaagtc cgccgtctat ccccaccgag gtgacgagtg atcaagcctc cactcgaggc
     3841 gctgctgggc cgggtagaga acaagtacgc tctggtcatc gtcgccgcca aacgggcccg
     3901 acaattgaag gaaggcgcgt tgcccatggt agatatcgat agtgcgaacc ccgtgacgat
     3961 agccctcgag gagatcgccg cggggaagat ccgctttgag ctgccgaaac tgggcctcaa
     4021 ataggcgaaa acccgcgaat agaacactgc gcccccctgc atacgtatag agaacagagt
     4081 gccgtacaat ggggtggtca ccgtgcaggg aatgctgatt ggactcgtac tggtcgcgct
     4141 ggtcgccgcc gcgcccgggg cgcccctgat ggtcgagcgt gccgatggcg ggctgcggca
     4201 cgaggcgtcg atatcaaagc gggcatttga tgcgggcgag tccatcgaag tcaccatcac
     4261 ggtgcgcaac gcgggctcga atccggtgac gttgacgttc ttcagcggac agcgattcga
     4321 tctgctcgtc aggcgccccc ggggcgatga aatctggcgg tggtcgcatg acaaagcctt
     4381 catccaaatt gtccagtcca tccccatccg gccaaacgag ccgctgacgc tcaaaggatc
     4441 ctgggatcag cgcgactacc aaggccgccg agccgatcca ggcacgtatg aagtcgtcgc
     4501 gttcgtgacc gggcggaccg aaggatccga gcgatcctcg attcaactcc cagccctgcc
     4561 gttcaccata cgataacgca ccgtagtata attgggtctg cgtggcggtg tagctcagct
     4621 ggtcagagca cgcggctcat acccgcggtg tcgggagttc gagtctcccc gccgccacca
     4681 cgtaccactc gcttcgctcg ggtacatggc ggcgccacaa ccaggcgcga agcttgcccc
     4741 gacctcgaac taagtgcgta gttcggggct ccggttcatg gcggcgccac taattcagag
     4801 atcggcgact ggttcatgtc ctgaacccga acggcgtgag tggttcgggg cacatctcga
     4861 ggcagccaac cgtggctgcc ttccacgttt atgaacgata tggatctttt tgagaaattc
     4921 aaggcaacaa ttgtagacca ccgcctgatc tcgaacggcg acacggtcat cgtcgccgtc
     4981 tcgggcgggg ccgactcgac ggcgctgctc catctcctcg cgtcgctgcg ggaccaatgg
     5041 aaccttacac tcgttgtcgc ccacctcgat caccgcctgc gggtggacag tgccgacgac
     5101 gcgcgttttg tgctccgaat ggcggccgag ctcggcgttg cgcccgtgac cgacgtcgcc
     5161 gacgtacgag gtttggcccg ccggcgcaag cgttcaattg aagaggcggg ccgcgaagcg
     5221 cggtatgagt tcctcgaacg tgtggcgcgt gagcacgggg cgcggcgcat tgcgactgcc
     5281 catactcaag atgatcagat cgagacggtg ctcatgcgga tcctgcgggg agatccgtgg
     5341 gatgcgcagg gcggcatccc gatcgtccgg ccgctgaacg cggcacaggt catccgtcca
     5401 ttgctgatgg gtgcgcgcga ggaggttcgg cgctacttgg cggggcggtc catcgagtgg
     5461 cgggaagatc ccagcaaccg tgatgtgcgg ttcgaccgca actggatcag actgcgggtg
     5521 ctgcccgatc tcgagcggct cactcctggc gtgcgcgagt tactccttgg gttcagcagc
     5581 gctgctcgac tcgcagatca tctgctgaac gaacttgccc atcagcacgc agcaaacgtg
     5641 gccgaagccg gcgagcatat gatcagaatt ccactgcccg agttccgccg gcatccccag
     5701 gacgtgcagc gccgaatgtt gaggtgggcc gcttctcaga tcaccggaac aaccaccaga
     5761 gggccggccg ggtgggaaaa gagggcgcat caggctgcca cacaaggccg cgccggtgat
     5821 gttgtagacg gcgcgatcgc agttcgggtc aactacgatg cgcttgaggt cgcccgtccg
     5881 caatcgcaga agccgagcag ggagtaccga ttaaagat
//
LOCUS       DBNGSB010000069         2447 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2192864, whole genome shotgun sequence.
ACCESSION   DBNGSB010000069 DBNGSB010000000
VERSION     DBNGSB010000069.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2192864"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..1959)
                     /gene="ftsH"
                     /locus_tag="AC159B_01930"
     CDS             complement(52..1959)
                     /gene="ftsH"
                     /locus_tag="AC159B_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR3376544.1"
                     /translation="MNRYLRNLVVWGIILAVVIYFFVGPLYRARTPREEISYSQFIEH
                     AEAGRVKEVTIGDETITGKLKDGTSEGATFRTYVPANDTSYLAILQARGTQINVEPRN
                     RNAIWPNILSTMLPFLLLIGLWMLMLRQAQSGSNQAMSFGKSRARLHTENRPKVTFDD
                     VAGVDEAKEELQEVIEFLKHPKKFQALGAKIPRGVLLVGPPGSGKTLLAKAVAGEAGV
                     PFFSISGSEFVEMFVGVGASRVRDLFEQAKKSAPCLVFIDEIDAVGRQRGAGLGGGHD
                     EREQTLNQLLVEMDGFDPNAGIILIAATNRPDILDPALLRPGRFDRRIVVDNPDSKGR
                     RAILDVHARGKPLAEDVDLEILAKHTPGFSGADLANLVNEAALLAARRNKKKVTMSEM
                     EEAIERVIAGPQRRSRVLSAKEREIAAYHEGGHALLAKLLPHADPPHKVTILPRGMAL
                     GYVISAPPEDKYTYTKNELLDRITAALGGRVAEELVFGEATTGAQNDFEQATELARRM
                     VTEFGMSEKLGPMALGKRHGPVFLGRDLIETRNYSEEIAYEIDKETRKIIDECYDHAR
                     QVITEARDKLERIAHALLEKESLEGAQLERVMAGEPLEPEPPAAAQPAAPPEARTDVP
                     IPSLKPKPEAS"
     gene            complement(2015..>2447)
                     /gene="tilS"
                     /locus_tag="AC159B_01935"
     CDS             complement(2015..>2447)
                     /gene="tilS"
                     /locus_tag="AC159B_01935"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF023162.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIR3376545.1"
                     /translation="IPGTVTAEEFGMMLSATIVETAAPARSSSPHSDEVYLDAGSLGE
                     ELRIRSWRSGDWMIPLGMRGKKKVQDLFVDDKVPRWERHRIPVVADARGRIVWIVGRR
                     ISEVARITTTTRHVVRLSVRKAAGLVLSVRNTPDDVVVGSA"
BASE COUNT          436 a          789 c          714 g          508 t
ORIGIN      
        1 aaggctgcct ggcaacaggc agccttcgtc acgacagaat ttcgtccctg gttaggacgc
       61 ttcaggctta ggcttcagcg acgggatcgg cacgtccgtc cgggcctccg ggggcgcggc
      121 gggctgagcc gcggcgggtg gttcaggctc gagcggttcg ccggccatca cccgttcgag
      181 ttgggcgcct tccagactct ctttctccaa cagcgcatgc gcaatgcgct ccagtttgtc
      241 gcgggcttcc gtgatcacct ggcgcgcgtg gtcgtagcac tcgtcgataa tcttccgcgt
      301 ctccttgtcg atttcgtacg cgatctcctc gctgtagttt ctcgtttcga tcaggtcgcg
      361 gcccagaaat accggcccgt ggcgcttgcc cagcgccatc ggcccaagtt tttcgctcat
      421 cccgaactca gtcaccatcc ggcgggcgag ttcggtcgct tgctcgaagt cgttctgcgc
      481 gccggtcgtc gcttctccga agaccaattc ttctgcgact cgcccaccca gagccgcggt
      541 gatgcggtcg aggagctcgt tcttggtgta tgtatatttg tcttcaggcg gcgcgctgat
      601 gacgtatccc agcgccatgc cgcgcgggag aatagtgacc ttgtgcggcg ggtcggcgtg
      661 agggagcagc ttggcaagca gtgcatgtcc gccctcgtgg taagcggcga tctcccgctc
      721 cttagccgac agcacacggc tacggcgctg cggtccggca atgacgcgtt cgattgcctc
      781 ttccatttcg gacatcgtga ccttcttctt attgcgccgc gcggcgagca acgccgcctc
      841 gttgacgagg ttcgccaggt ccgcgcccga aaatcccggc gtatgcttgg ccagaatttc
      901 gagatcaaca tcctcggcga gcggctttcc acgagcgtgg acatcgagaa tcgcccgccg
      961 tcctttactg tccggattgt cgacgacgat ccggcggtca aacctgcccg gccgcagcag
     1021 tgcaggatcg agaatatccg gacggttggt cgccgcgatc aggataatgc cggcgttcgg
     1081 atcaaaccca tccatttcaa ccagcagctg gttcagggtc tgctcgcgtt catcgtgccc
     1141 gccgcccaat ccggcgccac gctgccggcc caccgcgtcg atctcgtcga tgaagaccag
     1201 acacggcgcg ctcttcttgg cctgctcgaa caagtcacgc acgcgcgacg cgcccacacc
     1261 cacgaacatt tccacgaatt ctgaacctga gattgagaag aagggtacgc cggcctcgcc
     1321 ggccaccgct ttggccagca gcgtcttgcc gctcccaggc ggtccgacca gaagcacacc
     1381 gcggggaatc ttggcgccga gggcctgaaa cttcttgggg tgcttgagga actcgatgac
     1441 ttcctggagt tcctccttcg cctcatcgac gccggccacg tcatcaaacg tcaccttggg
     1501 tcgattctcc gtatgcagac gtgcgcggct tttgccgaac gacatcgcct gattgctacc
     1561 gctttgcgcc tgccgcagca tcagcatcca cagcccgatc agcaacagga acgggagcat
     1621 cgtcgagagg atgttcggcc agatggcatt ccggttgcgc ggctcaacgt tgatctgcgt
     1681 gcctcgtgcc tgaaggatgg cgaggtagga tgtgtcattg gcaggcacgt aggtccgaaa
     1741 cgtcgcgcct tccgaagtcc catccttgag cttgccggtg atggtctcgt cgccgatcgt
     1801 cacttctttc acacgccctg cttcggcatg ttcgatgaat tggctgtacg aaatctcctc
     1861 tcgcggggtg cgggcgcggt agagcggtcc gacaaagaag taaatgacga ccgccaggat
     1921 gataccccag acaaccaggt tgcggagata tcgattcagc accgctcttg cctcctacga
     1981 gttagcctta cgatacgacg aacggtttct cttatcaagc cgacccaacg acaacatcgt
     2041 cgggggtatt ccgtacagag agcacgaggc ctgccgcctt tcgcacactg agccggacga
     2101 cgtggcgcgt tgtagtggta attctcgcga cttcagagat acgccggcca acgatccaca
     2161 cgatccgtcc gcgcgcgtcg gcgaccacgg ggatcctgtg gcgctcccag cgcggcactt
     2221 tgtcgtcgac gaacagatct tgtactttct ttttgccccg cataccgagc gggatcatcc
     2281 aatcgcccga gcgccacgaa cgaatccgca attcctcgcc cagcgagccg gcatcgaggt
     2341 agacttcatc ggaatgaggg gaggacgaac gggccggcgc tgcggtctca acgatggtgg
     2401 cactcagcat catgccaaat tcctctgccg tcaccgtacc cggaatc
//
LOCUS       DBNGSB010000070         5626 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2040141, whole genome shotgun sequence.
ACCESSION   DBNGSB010000070 DBNGSB010000000
VERSION     DBNGSB010000070.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 5626)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5626)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5626
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2040141"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /gene="ruvB"
                     /locus_tag="AC159B_01940"
     CDS             complement(<1..753)
                     /gene="ruvB"
                     /locus_tag="AC159B_01940"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007790131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HIR3376546.1"
                     /translation="MTDPVAEEQFIRSLRPKSLEECIGQSKVIEGLRISIQAAREREE
                     ALDHVLLHGPPGLGKTTLANVVAYEMGANIVTTSGPALERGGDLMGILTNLQPKDVLF
                     IDEIHRLSRTIEEFLYPAMEDFVVNFVIEKGAHARTLRYQLPPFTLVGATTRAGLLSS
                     PLRERFGIFHHLDFFSVEDLIRVVKRSASILEIEITGDGALAIASRSRGTPRIANRLL
                     RRVRDFAQVRADGSITATVAAVALEFEGVDSRG"
     gene            complement(750..1382)
                     /gene="ruvA"
                     /locus_tag="AC159B_01945"
     CDS             complement(750..1382)
                     /gene="ruvA"
                     /locus_tag="AC159B_01945"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HIR3376547.1"
                     /translation="MIAYLKGPILRRAEEWVIVEAGGVGYEVHLPAVIARAIPQDRDG
                     VPSIIELHISYHATQNQPKPLLIGFLHEVEQEFFERFITVDGMGPTKAMKAIVHPIHA
                     IADAIERKDVQFLKRLPGIGERTAEKIVATLHGKMGKYALLRAPEAPTPVGEDFKEEV
                     MEVLTKQLGHRAAEARRMVEEALRRRPGMATAEELFQEVYRIEQADAVAR"
     gene            complement(1379..1876)
                     /locus_tag="AC159B_01950"
     CDS             complement(1379..1876)
                     /locus_tag="AC159B_01950"
                     /inference="COORDINATES: protein motif:HMM:NF014166.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HIR3376548.1"
                     /translation="MLILGVDPGLQVTGYGLVEGSPQGVRLIDCGLIRTSDDAPFASR
                     LHAIHREISLIVAQHTLSAIAVEDLYAAPRFPRTAILMGHVRGVVCQAAAACEVTVLS
                     LPPASVKRAIGGFGAASKGQMQLAIQRLFGLPSPPDAHCADAIGIALVALSREGVPQH
                     RVAFA"
     gene            complement(1878..2624)
                     /locus_tag="AC159B_01955"
     CDS             complement(1878..2624)
                     /locus_tag="AC159B_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR3376549.1"
                     /translation="MSGHSKWHNIRLKKGRADQIRGKLFSKLAREISVAAKEGGGNPD
                     GNARLRTAIERGREAGMPSDNIERSIQRGTGQVEGASYETITYEGYAQGGVALMVETL
                     TDNRNRTASEIRNVFTKNGGAMSEAGSVAWMFDRKGLITVDRSKAIEDDLLVAALEAG
                     AEDVRSVGDVYEIVTTPEQFFKVRQALEAAKIPVATSDLTMVPKSTVRVEGKEAQQVL
                     RLMEALEEHDDVQQAYANFDIPDEILQQVG"
     gene            complement(2644..4947)
                     /locus_tag="AC159B_01960"
     CDS             complement(2644..4947)
                     /locus_tag="AC159B_01960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR3376550.1"
                     /translation="MRELRRRFQLPPGRQWWRLPLIIGASVLGAAFIALGIVAGMTVS
                     FARSLPNVNALYNPPSQSTRIYASSGELIASLYRENRDIVKLSEIPETLQRAVIAVED
                     DRFYQHRGVDVRGTFRAMWRNLQARELIEGGSTITQQLARSIFLTQKRQVSRKLAEIM
                     LAVEIERRLTKAEILERYLNQVYFGQGAYGVEMAARVYFGKRAKDMDLGEGALLAGLI
                     RGPSIYSPYQNMKLAKEQQLVVLRRMVERGYITGEKARAVAARPIKLAAESNAGLVGI
                     RAPYFVSYILPYLLERYGEDLVYDGGLRVYTTLDLKMQIAAERVVRDGIDAARKNLKV
                     SQAALVAIDPATGYVRAMIGGYDFAQSQFNRAWQASRQPGSSFKPFIYVSALANGMSP
                     ARIIEDSPVTYNIPGSKVWKPKNYDGRFSGRVTMRQAVERSINIPAIKTLDELGPEQV
                     ITYAKRMGIKSPLQPNLSLALGAHEVTPLEMASAFGTLATLGLRTEPVAVRKIVSGDG
                     QVLEDNLPRQELVMSEDVAYVMTDLLRGVIARGTGRAAAIGRPAAGKTGTTDDYRNAW
                     FIGYTPNLTTAVWVGNDDNSPMRRVVGGTVPARIWAAFMKEATTELPPNDWRRPEGVI
                     TITVCGNTGLLAASACPNPRRESFIRGTEPNEYASGQTTGDGATVGDLPLRISSPGNG
                     QNVASPFQVEGTTVPGATVSLSILAQGGFLRISVAETYLPVTSEGNFSYLFRPSLQIP
                     GVQYVITIIATTADGSRSSTTITVSEQ"
     gene            complement(5045..5494)
                     /locus_tag="AC159B_01965"
     CDS             complement(5045..5494)
                     /locus_tag="AC159B_01965"
                     /inference="COORDINATES: protein motif:HMM:NF019095.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HIR3376551.1"
                     /translation="MSRSLRFGLLVFAVLFATGCNQLLAQVERFSTFLVGSAEVPPVA
                     TSATGTMALTIQGQSGSWVLTGQNFSSNVTAAHIHGPAGVGVIAGIIVTLAGAQPSNS
                     ISSTGTFGPANVSGMTWDTFVTHVRNGMTYVNVHTTTNPSGEIRGQF"
BASE COUNT         1048 a         1776 c         1652 g         1150 t
ORIGIN      
        1 cccgcgcgaa tcaactcctt caaactccaa cgccacggcc gcgaccgtgg ccgtaatcga
       61 tccgtcggcc ctgacctgcg cgaagtcccg cacccgtcgc aggagccggt tggcaatgcg
      121 cggtgtccct cgcgaccgcg atgcgatcgc cagcgcgccg tctccggtga tctcgatctc
      181 caagatcgac gcagatcgtt tcactacccg gatgaggtcc tcaaccgaga aaaagtcaag
      241 atgatggaaa atcccaaacc gttcccggag cggggaggat aacagtcccg cgcgcgtggt
      301 cgcgccgact aaggtgaacg gcggcagttg ataccggagt gtacgggcat gcgctccctt
      361 ctcgatcacg aagttgacca cgaagtcttc cattgccgga tacagaaact cctcgatcgt
      421 gcgtgagaga cgatgaattt cgtcaatgaa caggacatcc ttcggctgca ggttggtgag
      481 gatacccatc aggtcgcctc ctcgctccaa cgcgggaccg gaggtggtaa caatgttcgc
      541 ccccatctca taggcgacaa cattcgccag ggtggttttg ccgagacctg gcggcccatg
      601 aaggaggaca tggtcgagcg cctcttcccg ttctctcgca gcctgaatgc tgatccgcag
      661 cccttcgatc actttgctct ggccgatgca ctcttccagc gatttcggcc gcaggctccg
      721 gatgaactgc tcttcggcca cgggatcggt catcgcgcca ccgcgtctgc ctgctcgatg
      781 cgatacactt cctgaaatag ttcttctgcc gtcgccatgc cgggtcgacg ccgcaacgcc
      841 tcttcgacca tgcgccgggc ctcggctgcc cggtgaccca actgcttggt gagaacttcc
      901 atgacttctt ctttgaaatc ttcgcctacc ggtgtgggcg cctcaggcgc tcgtagcagc
      961 gcgtacttcc ccattttccc gtggagtgtc gcgacgatct tctcggctgt ccgctcgccg
     1021 atccccggca gccgcttgag gaactgaaca tccttcctct caatggcatc ggcgatcgcg
     1081 tgaatcgggt ggacaatcgc cttcatcgcc ttggtcggtc ccatcccgtc cacggtaatg
     1141 aaccgttcga agaactcttg ctcaacttcg tgcagaaatc cgatcagcag gggcttgggc
     1201 tgattctgcg tcgcgtgata ggagatgtgg agttcaatga tcgacggaac gccgtcccgg
     1261 tcttggggaa tcgcgcgggc aatgacggcc ggcaggtgca cttcgtagcc gacgccacct
     1321 gcctcaacga taacccactc ctcggcgcga cggagaatcg gccccttgag gtacgcgatc
     1381 aagcgaatgc caccctgtgc tgcggcacgc cttctctcga caacgcgacg agcgcgattc
     1441 cgatcgcatc ggcacagtgt gcatcgggtg gtgaaggcag cccgaacaac cgttggatgg
     1501 ccagttgcat ttggcctttg ctggcggcgc cgaatccccc gatcgctcgc ttgaccgagg
     1561 ccggtggcag cgacaggacg gtgacctcac aggcggctgc cgcctggcag acgacgcccc
     1621 gcacgtggcc catcaagatg gccgtgcggg ggaatcgagg ggctgcgtac agatcctcga
     1681 ctgcaatagc cgacaatgtg tgctgagcaa caatgagcga gatctcccgg tggatcgcat
     1741 gcagccggct ggcgaagggg gcgtcgtcgc tggtgcggat cagaccacag tcaatcagac
     1801 gaacaccctg cggggatcct tcaaccaggc cataccctgt aacctgcagt cctggatcga
     1861 cgccgaggat aagcaaccta ccctacttgt tgcaagattt cgtcgggaat gtcgaagttc
     1921 gcgtacgcct gctgcacgtc gtcgtgctcc tcgagcgcct ccatcagccg gagaacctgt
     1981 tgagcttcct tgccttcgac acggaccgtg gacttgggca ccatggttaa gtcggaagtg
     2041 gcgaccggaa tcttggcggc ctcgagggcc tggcgcacct tgaaaaattg ctcgggcgtg
     2101 gtcacgatct cgtagacatc tccgacgctg cgaacatctt cggctccggc ttcgagggcc
     2161 gcgaccagaa gatcgtcctc aattgccttg gatcgatcca cagtgatgag gcctttccgg
     2221 tcgaacatcc atgccaccga cccggcctcg ctcatcgcgc cgccgttctt ggtgaacacg
     2281 ttgcggattt cgcttgccgt ccggttccgg ttgtccgtta atgtctcgac catgagcgcg
     2341 acgccgccct gtgcataccc ttcgtaggtg atcgtctcat acgacgcgcc ttcgacctga
     2401 ccggtaccgc gctggataga ccgctcgatg ttatctgatg gcatcccggc ttcacgcccg
     2461 cgctctatcg ccgtccggag ccgcgcgttg ccgtcgggat ttcccccgcc ctctttcgcc
     2521 gccacgctga tctcacgggc cagcttgctg aacaacttgc cgcgaatctg gtccgcgcgc
     2581 cctttcttga ggcggatgtt gtgccatttg gaatgcccgg acatggtttc tcccctgacg
     2641 aggttattgc tctgagacgg taatcgttgt cgagctgcgc gacccgtccg ctgtcgtggc
     2701 gatgatcgta atgacgtact gaacgcctgg gatttgcagc gacggcctga agaggtaact
     2761 aaagttgcct tccgaggtca cgggcaaata agtctcggcg actgagatgc gcaggaatcc
     2821 cccctgcgcg agaatcgaga ggcttaccgt cgcgcccgga accgtggttc cctcgacctg
     2881 gaacggggat gccacgttct gcccgttgcc cggcgaggag atcctcaggg ggaggtcacc
     2941 gacggttgcg ccgtcaccag tcgtttgtcc cgaggcatac tcgttgggct cggttccgcg
     3001 gataaaggat tcccggcgcg ggttcggaca ggcgctcgcc gcgaggagcc cggtattccc
     3061 gcacacggtg atggtaatga cgccctccgg tcggcgccag tcgtttggcg gtagctccgt
     3121 ggtggcctct ttcatgaacg ccgcccaaat gcgagccggc accgtgccgc cgacgacgcg
     3181 tcgcatcggg ctattgtcgt cgttaccgac ccagacggcc gtggtcaggt tcggcgtata
     3241 gccgatgaac caggcattcc gataatcatc ggtcgtgccc gtcttccccg ccgctggccg
     3301 gcctatggcc gctgcgcggc ccgtccctcg cgcgatgaca cctctcaaga gatcggtcat
     3361 cacgtaggcg acatcttcgc tcatcacaag ttcctgccga ggcaggttgt cttcgaggac
     3421 ctggccatcg ccggagacga tcttgcggac ggcgacaggc tcggtccgaa ggccgagggt
     3481 ggcaagcgtc ccgaacgccg atgccatctc cagtggcgtg acttcatgcg ctccaagcgc
     3541 gagtgaaagg ttcggctgca gcggactctt gatacccatc cgctttgcgt acgtgatgac
     3601 ctgctcagga cccagttcgt cgagcgtctt gatcgcaggg atattgatcg atcgctcaac
     3661 cgcctggcgc atggtcaccc tgcctgagaa tcgcccgtcg taattcttag gcttccatac
     3721 tttggacccc gggatgttgt acgttaccgg ggaatcctca atgatccgtg ccggtgacat
     3781 tccgttggcc agcgcgctta cataaatgaa cggcttgaag gacgaccccg gctgccgact
     3841 cgcctgccag gcgcggttga actgactctg cgcgaaatcg tacccaccga tcatggcccg
     3901 gacatatccg gtagcgggat cgattgccac tagcgcggcc tgcgacacct tcaagttctt
     3961 ccgggccgcg tcaatgccgt cgcggaccac gcgttcggcg gcgatttgca tcttgagatc
     4021 gagcgtggtg tagacgcgaa gacctccatc gtagacgagg tcttcgccgt agcgctcgag
     4081 caggtacggc agaatgtagg agacgaaata cggcgcccgg atgcccacca gacccgcgtt
     4141 gctctctgcc gccagcttga tcggccgggc ggccaccgct cgcgcctttt cccccgtgat
     4201 gtacccacgc tcaaccatcc gccgcagcac gaccagctgc tgctctttgg ccagcttcat
     4261 gttttggtaa ggtgagtaaa tggatgggcc gcggatcaac ccggccagca gcgcaccttc
     4321 gccgagatcc atgtccttgg cgcgctttcc aaagtacacg cgagccgcca tttctacgcc
     4381 ataggcgccc tgaccgaagt acacctgatt caggtaccgt tcgaggatct cggccttagt
     4441 tagacgccgc tcgatctcga ccgccagcat gatctccgcg agcttgcggc tgacctggcg
     4501 cttctgtgta aggaaaatac tcctggcaag ctgctgggtg atcgtacttc cgccctcgat
     4561 caattccctg gcctgcaggt ttcgccacat cgcgcgaaac gtgccgcgca cgtcaacgcc
     4621 gcggtgctga taaaaacgat catcttcgac ggcgatgaca gcccgctgca gcgtctcggg
     4681 gatttcgctg agcttcacga tgtcgcggtt ttcgcggtag agactcgcaa tgagctctcc
     4741 actactcgcg taaatccgtg tggactgact cggcggattg tagagcgcgt ttacgttcgg
     4801 gagggacctc gcgaaggaga ccgtcattcc cgcgacaatg ccaagggcaa tgaaggcggc
     4861 cccgagcacg ctggcgccga taataagagg aaggcgccac cactgccggc ctggaggaag
     4921 ctggaacctg cgtctgagtt ctctcacgat aaactctaac cttcctcata gtataccgcg
     4981 gaaaatcaga atgccctgga cacagtccag ggcattctgc cgcgaagact ggtggttcag
     5041 ggcatcagaa ttgaccgcgt atttcgccgc ttgggttcgt ggtggtgtgc acgttgacgt
     5101 aggtcatacc attgcggaca tgggtgacga aagtgtccca agtcatccca ctcacgttcg
     5161 ccgggccgaa ggttccggtg gaacttatgg agttgctcgg ctgtgccccg gcaagcgtca
     5221 cgataatccc cgcaatcacg cccacccccg cgggaccgtg gatgtgcgcg gcggtcacgt
     5281 tgctcgaaaa gttctgcccg gtgagaaccc agctcccgct ctgaccttga atggtaaggg
     5341 ccatggtccc cgtggcgctt gtcgcgacgg gaggcacttc ggcgctcccg accagaaatg
     5401 tcgaaaaccg ttccacttgt gccagtagct gattgcatcc ggtggcgaat agaacggcga
     5461 acaccagcaa tccgaaacgg agcgacctag acatgtggac tgcccccctt caccttatct
     5521 gcctgcgcgt ggtgaacaca gcatttctct gcctcgtcac aactcagtca cgatttgggg
     5581 gccggagcga acgacctgag gaactaaaat atttggtgga gacgag
//
LOCUS       DBNGSB010000071         5141 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_199431, whole genome shotgun sequence.
ACCESSION   DBNGSB010000071 DBNGSB010000000
VERSION     DBNGSB010000071.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 5141)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5141)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5141
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_199431"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..306)
                     /locus_tag="AC159B_01970"
     CDS             complement(139..306)
                     /locus_tag="AC159B_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376552.1"
                     /translation="MEPDPKFVQEMTRRQMLRRAALLAGGAVAGPALARLFGAAGGGS
                     LAYAQGLVERV"
     gene            complement(321..899)
                     /locus_tag="AC159B_01975"
     CDS             complement(321..899)
                     /locus_tag="AC159B_01975"
                     /inference="COORDINATES: protein motif:HMM:NF021996.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HIR3376553.1"
                     /translation="MRRRWLWWVIAVVVIVGVPLAWYLGSPLFINRTVTEAFPMSAKA
                     TLPEGMTREQADTELMEASKQTSASSEAMPATAIATLAKGSFTDVDSFHKGSGTAQVA
                     RVGSDRVLRLEEFRVTNGPDLYVYLAAHPQPRSRAELDQGHVSLGRLKGNIGSQNYSI
                     PNSVRLEAYQSVVIYCRRFHVLFSTATLRPSP"
     gene            complement(1005..2078)
                     /locus_tag="AC159B_01980"
     CDS             complement(1005..2078)
                     /locus_tag="AC159B_01980"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3376554.1"
                     /translation="MRRSLLWKLLGINLLVIGVAVVVAAVTIGQFADAIFMRLMKEFS
                     LQTDVIHGLFTQTLLRSLVLASLIAGALGLLLSVFLFRRVVNPVREMEAMAARIAAGD
                     YGARVTVRSADELGALAESLNQMADSLATLERLRKDLVANVAHELRTPLTNLRGYLEA
                     IREGVAPASAEMIGSLHEDVMRLVRLVEALHALTLLDARLPKLQLEEVDLKVLLDRLV
                     ELYRAQFLSRGITVDKQLAAVGAVRVDSDLVAQAAQNLLDNALKYTPEGGRVDVGMSA
                     SDGSVRIAVSNTGDGIAPEDLPFIFERFYRSEKSRSRESGGAGIGLAIVKEVATVHGG
                     RVGAKSVDGLTMVWFTLPGSHAS"
     gene            complement(2121..2798)
                     /locus_tag="AC159B_01985"
     CDS             complement(2121..2798)
                     /locus_tag="AC159B_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020640813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3376555.1"
                     /translation="MTQTILVVEDDAKIVNLLRLYLEREGFAVAAAGDGHAALEAAEQ
                     ATPALVILDLMLPQMDGIELCRRLRARSDVPILMLTARVDEVDKLVGLSMGADDYVTK
                     PFSPREVVARVRTILRRTTREPRQRVMQRGDLVMDLDRHRVEAAGREVQLTPVEFRLL
                     QTLLQSPEHVFTRDALLNHMYANHEAAVVDRTIDVHIGKLREKLGDVPAKPRYIATVR
                     GLGYKLL"
     gene            complement(2844..3449)
                     /locus_tag="AC159B_01990"
     CDS             complement(2844..3449)
                     /locus_tag="AC159B_01990"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012549.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HIR3376556.1"
                     /translation="MSWELAEKLKARFPEFELFPRPEPHETPKESAPAQAAAAAPRRP
                     AHEAPPAYLIPGGVVPREKLLEIARVVATDADFELSYLSFVTAVDRPEAGEFEVVYHC
                     YAHQTLEELALKVRIPRDRPTVPSVTSIWDGANWHEREAYDLFGIVFEGHPNLRRIMM
                     TDDWIGHPLRKDYVYQDPAWLVEVAKRRKEEIEGLGLGERA"
     gene            complement(3446..3952)
                     /locus_tag="AC159B_01995"
     CDS             complement(3446..3952)
                     /locus_tag="AC159B_01995"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NuoI/complex I 23 kDa subunit family protein"
                     /protein_id="HIR3376557.1"
                     /translation="MSMQMPADGKKGLAEQAWQGAVGLVVGLKSTLRTMFERKITVSY
                     PIQKVNVSPRWHGLLALPIDQETDNDKCIICFQCERVCPDRCIHIEATGKAKDRLLTR
                     FDIEMDKCCYCGPCVEVCPTEAIVFVPHYEASTYNRANLLYDLEDLHRAAPKEPIATG
                     IVKVRKQR"
     gene            complement(3949..5052)
                     /locus_tag="AC159B_02000"
     CDS             complement(3949..5052)
                     /locus_tag="AC159B_02000"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HIR3376558.1"
                     /translation="MALRTEELLLNMGPQHPSTHGVLRLVVTLDGENIVDVQPDIGYL
                     HSSVERMMEYRTYLQNIALVDRGMDYLSAMANEEVWCLATERLAKVDVPERARYIRTI
                     MLELQRISSHLIWLGTFGIDLGAFTAFLWAMRERERIMDLFESATGGRLHHVYFRIGG
                     VYEDLPDGWTERCAEFCDYFLDRLPEYDELLTGNPIFLARTQGIGILSQEKAIAMGAS
                     GPVARASGIAYDVRKAHPYEVYDRVQFDMPVRQEGDCYARYLVRLEEMRQSVRIIRQC
                     LEALPAGEIRAKVAVTLKVPKGEVYARTESPRGDLGIYLVGDGSDRPYRVKTRAPAFS
                     NLYALTEMMRGWKVADVIAILGSIDIVLSDVDR"
BASE COUNT          940 a         1666 c         1524 g         1011 t
ORIGIN      
        1 ggtcagcttg gtgggacggc tcaccaggcc ggcgatctcg agattccagc ggtccacatt
       61 gacaaccgga tcaaatcccg gcgggttctt gctgaccacg tagaaatctt tgttggacgt
      121 cacctccggc gggagcccct agacgcgctc gaccagcccc tgcgcgtacg ccagggagcc
      181 tcccccggct gcgccgaaga gcctcgccag cgcgggtccc gccaccgccc cgcccgccag
      241 taacgcggca cggcggagca tctgccggcg cgtcatctcc tgaacaaact tcggatcagg
      301 ttccatttat gcacctcatg tcaagggctc gggcgcaggg tcgcggtcga gaataagacg
      361 tggaacctcc gacagtagat tacgacggat tgatacgctt cgagccggac gctattaggt
      421 atcgagtagt tctgcgaccc gatgttgccc tttagccggc cgagactgac gtgcccttgg
      481 tcgagttcgg ctctggacct cggctgaggg tgcgccgcga ggtagacata gagatcggga
      541 ccgttggtca cgcggaactc ctcgagccgg agcacccgat cagacccgac gcgcgccacc
      601 tgcgctgtgc ctgatccttt atgaaagcta tccacatcgg tgaaagatcc ctttgcgagc
      661 gtagcgatcg cagtcgccgg catggcctcg ctcgatgctg atgtctgctt gctggcctcc
      721 attagctcgg tgtccgcttg ctccctggtc atgccctccg ggagggttgc cttcgcgctc
      781 atcgggaacg cctcggtcac ggtccggttg atgaacaggg gtgagccgag ataccaggcc
      841 aacggcacgc cgacgatcac cacgacagca atgacccacc acaaccatct gcgcctcatc
      901 gacatttcct cccggacccc gggcctcgga ccccggaccc cgtcagtctt cccagccagt
      961 ctgcgccgcg gcagtgaacg tcagacaaac gctgtgtaaa gaattcatga agcatgcgac
     1021 cccggcagtg tgaaccatac catggtcagg ccatccacac tcttggctcc tactcgcccg
     1081 ccatggacag tggcgacctc cttcacaatc gcgagcccga tacctgcgcc tccggattca
     1141 cgggagcgag acttttcgct tcggtagaag cgctcgaaga tgaacggcag atcctcgggc
     1201 gcgatcccgt ctcccgtatt gctcacggcg atgcgaaccg acccgtctga agcgctcatg
     1261 cctacatcca cccgcccgcc ctcaggcgtg tacttcagcg cattgtcgag caggttctgc
     1321 gcagcctgcg ctaccagatc ggagtctacc cgcactgctc ccacggctgc cagctgcttg
     1381 tcaacggtga tcccgcgtga gaggaactgc gcgcggtaca gctcgacaag gcggtctaac
     1441 agcactttca ggtccacctc ttcaagctga agtttcggca gcctggcatc cagcagcgtt
     1501 agggcgtgca gcgcttccac gagccgcacc agccgcatga catcctcatg gagggaacca
     1561 atcatctcgg ctgaagcggg cgctactccc tctcgaatcg cctccagata cccgcggaga
     1621 tttgtgagcg gtgtgcgcaa ctcgtgggcc acgttcgcaa ctagatcttt gcgaagccgc
     1681 tccaaggtcg cgagtgaatc cgccatctgg ttcagcgatt cagcaagcgc ccccaactcg
     1741 tccgccgaac gtacagtcac ccgcgccccg tagtctccgg ccgcaatcct cgccgccatc
     1801 gcttccatct cccgcaccgg gttgacgacc cgccggaaca ggaacacgct gagcagcagg
     1861 ccgagggcgc cggcaatgag gctggcaagg accaacgaac gcaggagtgt ctgggtgaac
     1921 aggccgtgta tgacatctgt ctggagactg aattccttca tcagtctcat gaagatggcg
     1981 tcggcgaact ggccgatcgt gacggcggcg acgacgaccg ccaccccgat cacaaggagg
     2041 ttaatcccga gtagtttcca cagcaggctt cgtctcattt ttgcttcgag tttgcgcgct
     2101 tacgcgtttg cgcgtttgcg tcagagcaat ttatatccca gcccccgcac cgtcgcgatg
     2161 tatcgaggtt tcgctggaac gtcgcccagt ttctcccgca gcttgccgat gtgaacgtcg
     2221 atcgttcgat ccacgacggc ggcttcatgg ttcgcgtaca tgtggttgag cagcgcgtcg
     2281 cgtgtgaaga cgtgctctgg tgattgcaga agcgtctgca gcagcctgaa ctcaaccggg
     2341 gtgagctgga cctctcggcc cgcggcttcg acgcggtgcc ggtcgaggtc catcaccaga
     2401 tcgcctcgct gcatgaccct ttgtcggggc tcacgcgtgg tgcgtcggag gatggtcctg
     2461 acccgcgcca cgacctccct cggactaaag ggtttggtca cgtaatcatc cgcgcccatc
     2521 gagagtccca ccagcttatc tacctcatcg actcgagccg tcagcatgag gattggaacg
     2581 tctgacctcg cccgcaggcg ccggcacagc tcgatgccgt ccatctgcgg cagcatgagg
     2641 tcgaggatca cgagagcagg cgttgcctgc tccgccgcct cgagcgcggc atgtccgtcg
     2701 ccggcagcgg ccacagcgaa cccttcccgc tccagataga ggcggagaag gttgacaatc
     2761 ttggcgtcat cctcgacgac aaggatggtc tgcgtcatct gacgttcacg ttatgaatcg
     2821 attgtaaagg gaagttaaag gggttaggct ctctcgccta ggccgagccc ttcgatctct
     2881 tctttgcggc gcttggcgac ctcgacgagc caggccggat cttgatatac gtagtccttg
     2941 cggagcgggt gaccgatcca gtcgtcggtc atcatgatgc ggcgcaggtt tggatgcccc
     3001 tcgaagacga tgccgaagag gtcgtacgct tcacgctcat gccagttcgc gccgtcccag
     3061 atggacgtca cggagggcac ggtcggccga tcgcgcggga tgcgaacctt gagggcgagc
     3121 tcctcgaggg tctgatgcgc gtagcaatgg tacacgacct caaactcgcc cgcttccggt
     3181 cgatccaccg cggtcacaaa tgacaggtat gagagctcga agtcggcgtc ggtcgcgacg
     3241 acgcgggcaa tctcgaggag tttttctctc ggcacgacgc cgcctgggat tagatatgcg
     3301 ggcggcgcct cgtgcgccgg acgtcgcggt gccgccgcgg ccgcctgtgc aggtgcactc
     3361 tcctttggtg tttcgtgggg ctccgggcga ggaaagagct cgaactccgg gaaacgggcc
     3421 ttgagtttct cagccaactc ccagctcatc gctgttttct cactttcacg ataccggtcg
     3481 cgatcggctc tttcggcgcg gcgcggtgca ggtcttcgag atcgtacagc aggttggccc
     3541 gattgtatgt gctggcctcg tagtgcggga caaacacgat tgcttcggtc gggcagactt
     3601 ccacgcacgg cccgcagtag cagcacttgt ccatctcgat atcgaatcgc gtcagcaaac
     3661 ggtccttggc tttcccggtc gcctcaatgt ggatgcaacg atccggacac acgcgctcgc
     3721 actggaagca gatgatgcac ttgtcgttgt cggtttcctg gtcgatcggg agggccagca
     3781 gcccatgcca gcgcggcgag acgttcacct tctgtatcgg gtagctgacc gtgatcttcc
     3841 gctcgaacat ggtacgcagt gtcgacttga gcccgaccac aaggccgacg gcaccttgcc
     3901 acgcttgctc ggccagaccc ttcttcccgt ctgcgggcat ctgcatgctc atcgatccac
     3961 gtccgagagc acgatgtcga tacttcccaa gatggcgatg acgtccgcta ctttccaccc
     4021 gcgcatcatc tcggtcaacg catagaggtt gctgaacgcc ggcgcgcggg tcttcacacg
     4081 atagggacgg tctgatccgt cgccgaccag atatatcccg agatccccgc gcggcgactc
     4141 cgtgcgcgcg taaacttcgc ccttgggcac cttcagggtc accgccacct tggcgcggat
     4201 ctcccccgcc ggcagggctt ccaggcattg ccggatgatg cgaacgctct ggcgcatttc
     4261 ctccaaccgc accaaataac gcgcgtagca atcgccttcc tggcgcaccg gcatgtcgaa
     4321 ctggacgcgg tcgtagactt cgtaggggtg cgctttccgg acatcgtagg cgatccccga
     4381 cgcacgcgcc actggaccag aagcacccat cgcgatggct ttctcctgtg aaaggatccc
     4441 gatgccctga gtcctcgcga gaaagatcgg gttcccggtc agcaactcgt cgtactcggg
     4501 cagcctgtcc aggaagtagt cgcagaactc ggcgcatcgc tccgtccaac catccggcag
     4561 atcctcgtag acgcccccga tccggaagta cacgtggtgt aggcggccgc cggtggcact
     4621 ctcaaagagg tccatgatgc gctcgcgctc ccgcatcgcc cacagaaacg ccgtgaacgc
     4681 gccgagatcg atgccgaagg tgccgagcca gatgagatgg ctgctgatcc gctgcagttc
     4741 cagcatgatg gtccggatat agcgggcgcg ttcaggaacg tcgactttcg ccaggcgctc
     4801 ggtcgcaaga caccagacct cttcgttggc catcgccgac aggtagtcca tcccgcggtc
     4861 caccagcgcg atgttttgca ggtaggttcg gtactccatc atccgctcga ccgaagagtg
     4921 caggtacccg atgtcgggct gcacgtcgac gatgttctca ccgtcgaggg tgaccacgag
     4981 ccgcagcacc ccgtgggtgc tcgggtgctg cggccccatg ttgagcagga gttcttcggt
     5041 tcgaagagcc atgcggttac gtctgagccg cggatgtcgc gatgcggcgg cgcaggaacg
     5101 gttccttttt gatcttgtcc tgcagcttca ggaagccttc g
//
LOCUS       DBNGSB010000072         3407 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2348632, whole genome shotgun sequence.
ACCESSION   DBNGSB010000072 DBNGSB010000000
VERSION     DBNGSB010000072.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2348632"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..1259
                     /locus_tag="AC159B_02005"
     CDS             18..1259
                     /locus_tag="AC159B_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014789229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase-like domain-containing protein"
                     /protein_id="HIR3376559.1"
                     /translation="MREFYSAGARVMTRSLIRELLKLTRLGGIISFAGGLPDPATFPV
                     NELKDITTDILDKQPHLALQYGPTEGDVHLKDQLVKWMAKDGINVTRDQILITNGSQQ
                     GLDLVGRVFLDPGDAVILELPSYMAGLQVFRSYRAEMIGVPQDDAGMRTDLLEQTLAR
                     LAREGRRPKFIYVVPDFQNPSGVTMTEERRRMVLELARAHRALVVEDNPYRELRFEGP
                     PVSPLMSLDREGRVIYLSTFSKTLAPGLRIAWMAASEELIRKFVTAKQGVDLCCPSLT
                     QAIAAEYCARGHIYAHLPRITSLYRKKRDVMMRALEREMPPGVSWTKPEGGLFLWVRL
                     PEGMDTEQMLSSAVEDEGVAYVVGSGFHADGGGKNAMRLNFSYPSEEDIEEGIKRLAR
                     LVKKRLPAEYAREPSRGPRSP"
     gene            complement(1177..2502)
                     /locus_tag="AC159B_02010"
     CDS             complement(1177..2502)
                     /locus_tag="AC159B_02010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR3376560.1"
                     /translation="MTKAKEPQPKKPDAPAPPASRSPRPRRAWWVHARWLLLALPILG
                     LGFGLGMALYIFSHARAYTGSQEVQRPAEDVTSFTWPLRLGTRVNVLLIGVDVSLDNR
                     RQVLPVSRSDTLMLVSFDPVRNRVSGLSIPRDTRVLIPRFGENKANAAYAYGGPGLAV
                     RTVANFLGVPIHYYVKLGPQSFAKIIDAIGGVEIDVEKDMKYTDTWAGLHINLKKGRQ
                     LLDGERAMHYARFRHDAAGDIGRVERQQKVLLALFHKLKSASTVLNAPQLLRAFSENT
                     QTNLTFNELITLGAFTARLENSDLKFATLPGTNSIAYWDPDPSKMRQIVAEMFYGVDA
                     SIVASTQIEVLNGSGVPGLGRQMAVRLQAMGFQIVRVDNAPSVVDSTTIIDRTGRPGV
                     ARLVAEVLGRSRIKNQPGIGPDLTVIVARDLARGHTPPAGASSRVSPAF"
     gene            2677..>3407
                     /gene="ispE"
                     /locus_tag="AC159B_02015"
     CDS             2677..>3407
                     /gene="ispE"
                     /locus_tag="AC159B_02015"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HIR3376561.1"
                     /translation="MSLEVGKAIEGGKPVKELHLNAYAKINLTLDVLEARSDGYHDIE
                     TVMHTIALHDSVTLREAGAGVEVIVHDGEVPNDHRNLVMRTAQLLRETFNVDRSVQIE
                     LTKRIPVAAGLGGGSADAAITLIGLAQMWKLRLDGRELLALSLKLGSDVPFFMEGGAA
                     VARGRGERLTLLPPLPTTWVVLARPQIPVSTTWAYKQISGSHVGRRPNTSAMITAVQH
                     EDVREAGRLLSNVFEDVIGGAHPIIG"
BASE COUNT          626 a         1050 c         1093 g          638 t
ORIGIN      
        1 aactttccag cgacgacctg cgcgagttct actcggccgg tgctcgcgtc atgacgcgtt
       61 cgctgatccg ggagctgctc aagctcacga ggctcggcgg catcatctcg ttcgcgggcg
      121 gtctgcccga ccccgccacg ttccctgtca acgaattgaa agacatcacg accgacatcc
      181 tcgacaagca gccgcatctc gccttgcagt acggtccgac cgaaggtgac gttcatctca
      241 aggatcagtt ggtcaagtgg atggccaagg acggtatcaa cgtcactcga gatcagattc
      301 tcatcacgaa cggatcccag cagggcctcg acttggtggg ccgggtgttc ctcgatcctg
      361 gcgatgcggt gatcctcgag ctgccgtcct acatggccgg gctgcaggtg ttccgcagct
      421 accgtgcgga gatgatcggc gtcccgcagg atgacgccgg catgcgcacc gatctgctgg
      481 agcaaacgct cgcaaggctt gcccgcgagg ggcgtcggcc aaaatttatc tacgtggttc
      541 cggatttcca gaacccgtcc ggcgtcacga tgaccgagga gcgccgccgg atggtactcg
      601 agcttgcgcg ggctcaccgg gcactggtcg tcgaagacaa tccgtaccga gagctgcggt
      661 tcgagggccc gccggtctcg ccgctcatgt cgctcgatcg cgaggggcgc gtcatctatc
      721 tctccacgtt cagcaagacg ctcgctccgg gactgcgcat cgcgtggatg gcggcctccg
      781 aagaattgat ccgcaagttt gtcacggcca agcagggcgt ggatctctgc tgcccgtcgc
      841 tgacgcaggc catcgccgcg gagtactgcg cccgcggcca catttacgcc catctgccac
      901 gcattacgtc gctctaccgc aagaaacgcg atgtcatgat gcgtgcgttg gagcgggaga
      961 tgccgcccgg tgtctcgtgg accaagcctg agggcggatt attcctctgg gtcagactgc
     1021 ctgaaggcat ggatacggag cagatgctga gcagcgccgt ggaagatgaa ggcgtggcct
     1081 acgttgtggg gtcaggattt cacgccgacg gtggcgggaa gaacgcgatg cggctgaact
     1141 tctcgtaccc gtcagaagaa gatatcgagg aagggatcaa aaggctggcg agactcgtga
     1201 agaagcgcct gccggcggag tatgcccgcg agccaagtcg cgggccacga tcaccgtgag
     1261 gtcgggcccg atgcccggct ggttcttgat gcgggaacgg ccgaggacct cggccacgag
     1321 ccgtgcgacc ccagggcgtc cggtgcggtc gataatcgtc gtagagtcga cgacggacgg
     1381 cgcattgtct actcggacga tctggaagcc catggcctgc aggcgcactg ccatctgcct
     1441 gccaagcccc ggcacgccgc tgccgtttaa cacttcgatc tgcgtggatg cgacgatcga
     1501 tgcgtcgacg ccgtaaaaca tctcggcgac tatctggcgc atcttagagg gatccggatc
     1561 ccagtacgcg atcgagttgg tgccgggcag cgtcgcaaac ttcagatccg aattttccag
     1621 acgcgccgtg aacgcgccga gcgtgatcaa ctcgttgaac gtcaggttgg tctgtgtgtt
     1681 ttcggagaac gctcgcagca gctggggcgc gttcagcacg gtcgaggcgc tcttgagctt
     1741 gtggaacagc gccagcagga ccttttgctg gcgctcaacg cggccgatgt cgccggcggc
     1801 gtcatggcgg aagcgcgcat agtgcatggc ccgttcgccg tcgagcagct gccggccctt
     1861 tttcaagttg atgtgcagcc ccgcccacgt atccgtgtac ttcatgtcct tctcgacgtc
     1921 gatctccacg ccgccgatcg cgtcgatgat tttggcaaac gactgtggtc ccagcttcac
     1981 gtagtagtga atcggcactc ccaggaagtt ggcgaccgtt ctcaccgcga ggcccggacc
     2041 gccgtaggcg tatgcagcat tcgccttgtt ctcgccaaac cgcgggatca gcacccgggt
     2101 gtcgcggggg atcgaaagcc cactgacgcg gttgcgaacc gggtcaaagc tcaccagcat
     2161 cagcgtgtca gacctcgaga ctggaagcac ctgtcgcctg ttgtccagcg acacatccac
     2221 gccgatcaac aggacgttga cgcgggtgcc cagccggaga ggccacgtga acgacgtcac
     2281 gtcctcggcg ggacgctgga cctcctggct gccggtgtag gcgcgggcat gggagaagat
     2341 atagagtgcc atccccaaac cgaagccgag gcccaggatc gggagggcca gcagcagcca
     2401 ccgcgcgtgc acccaccagg ccctccgcgg gcgtggcgag cgggacgccg ggggtgccgg
     2461 cgcgtcgggt ttcttgggtt ggggttcttt ggccttcgtc acaggagttg ttccaagcga
     2521 tacctaatta ccataacata cctgcctcgg ggtggaggta tcgttattct cgtcccttcg
     2581 tgccggtatc ctgcttcggc acccctctct attgtcgtac ccgtccggac ccccattttg
     2641 ctcgtagatc cttactctgg tgtggaggtg tgagccgtgt cgcttgaggt tggaaaggcg
     2701 atcgaaggag gcaagcctgt gaaggagctc catctcaatg cgtacgccaa gatcaatctc
     2761 acgctggacg tgttggaggc gcgcagcgac ggttaccacg atatcgagac cgtcatgcac
     2821 accatcgcgc tccatgactc tgtgacgctt cgcgaagcgg gcgcgggtgt tgaggtcatc
     2881 gtgcacgatg gcgaagtgcc gaacgaccac cgcaacctcg tgatgcgcac cgcccagctg
     2941 ctgcgcgaga ccttcaacgt agatcgcagc gtgcagatcg agctcacgaa acgaatccct
     3001 gttgccgccg gactgggtgg cggcagcgcc gacgcggcta tcacgctgat cggactcgct
     3061 cagatgtgga aactgcggct cgatggccgc gaattgctgg cgctctccct caagctcggc
     3121 tccgacgtgc cgttcttcat ggaaggcggc gcggccgttg cgagagggcg cggggaacgc
     3181 ctcacgcttc tccccccgct gcctacgacc tgggtggtgc tggcgcgacc acagattccg
     3241 gtcagcacga cgtgggctta caagcagata agcggaagcc atgtgggaag acggcccaac
     3301 acctcggcga tgatcaccgc ggtgcagcat gaagacgtgc gcgaggccgg ccgcctgctg
     3361 agcaatgtct tcgaagatgt gatcggggga gcgcacccca tcatcgg
//
LOCUS       DBNGSB010000073         7414 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2809695, whole genome shotgun sequence.
ACCESSION   DBNGSB010000073 DBNGSB010000000
VERSION     DBNGSB010000073.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 7414)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7414)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7414
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2809695"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /gene="tatC"
                     /locus_tag="AC159B_02020"
     CDS             complement(<1..475)
                     /gene="tatC"
                     /locus_tag="AC159B_02020"
                     /inference="COORDINATES: protein motif:HMM:NF013097.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033281 - TAT protein transport complex
                     [Evidence IEA];
                     GO_function: GO:0009977 - proton motive force dependent
                     protein transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA];
                     GO_process: GO:0065002 - intracellular protein
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HIR3376562.1"
                     /translation="MEDRPMTLIEHLEELRRRLLISIAAFFIGMVLSWLFVDRILAAL
                     IRPIGHVVFLAPTEAFFVRLKVAALAGVFLGLPVILVQIWLFVSVGLTRTERRYTLSL
                     LPFSLMLFVAGALFAFLAILPVGVRFLLSYQTPDLVPMISVAAYTSFATAFVLAFG"
     gene            complement(582..764)
                     /locus_tag="AC159B_02025"
     CDS             complement(582..764)
                     /locus_tag="AC159B_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033281 - TAT protein transport complex
                     [Evidence IEA];
                     GO_function: GO:0008320 - transmembrane protein
                     transporter activity [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA/TatB"
                     /protein_id="HIR3376563.1"
                     /translation="MPNIGWPELIVILVIALLIFGPQRLAGIGSAMGRAIREFRGAVR
                     DAENEIDKSTTPDKRS"
     gene            complement(1056..1448)
                     /locus_tag="AC159B_02030"
     CDS             complement(1056..1448)
                     /locus_tag="AC159B_02030"
                     /inference="COORDINATES: protein motif:HMM:NF018980.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIR3376564.1"
                     /translation="MEDKKGPEVRGTRVNRILHNIVALVRARLEQAQREGQAVVRGLI
                     TGAVLVVVAIVLLALSVPLAVVTVILLLAEVMPPWGATGIVFAAMLAVAGVLLLVARS
                     KFRRKGFQVIRDLGQDFITMRESLTREA"
     gene            complement(1522..2502)
                     /locus_tag="AC159B_02035"
     CDS             complement(1522..2502)
                     /locus_tag="AC159B_02035"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HIR3376565.1"
                     /translation="MPQGVVAVTGASGFVGSLLTRALAGAGWTVRAIVTSAVAADRLR
                     ALGHQVALGDVRDRRSLDAGLAGCRAAVHLVAILRESAGETYDSINRQGAGNVADAAK
                     TAGVDRLIHFSVLGAGPSATRYLRSKWAGEQEVRDSGVRQVIFRPSFIMGPGGGAAEQ
                     FAQAVRFGPWYPLRLMLGRDRVLAAFANLTPVIPILGSGQTRFMPIDHRDLFTAVLQA
                     LERDDILGQTFELGGPDAVSYDQIMDTVTEVLRLRRWNLHIPRGVASIIVRIFHLLPN
                     PPITPDEFETLLVNNVCDNTNAVQTFRLSLRPFAVSLRDALLSEAEELKS"
     gene            complement(2502..3410)
                     /locus_tag="AC159B_02040"
     CDS             complement(2502..3410)
                     /locus_tag="AC159B_02040"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012568.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR3376566.1"
                     /translation="MEQELGSDDPFIAELVRHVLQTRGKMLRPALVCLSAQASGGAGD
                     ARLWIAAAVELIHIASLIHDDIIDDADLRRGEPTVNVQWGNQIAVLLGDYLFAKAFDL
                     LSRVRHPEVAPTMALATVKMSQAEIMQIKYGQQPHDDEDIYFRIIAGKTAHLFSAACK
                     AGALVAGNAHAAALLSTYGLEWGMAFQITDDALDLTSREESLGKPIHSDIEGGKVTLP
                     VIRALRDATPEDRDHLVHLLSGPARDGQREELFQLLRRYHGIDYALDVAARYSRQAVT
                     ALTPLPDGAAKDSLVRLAEFVVVRTR"
     gene            complement(3541..3918)
                     /locus_tag="AC159B_02045"
     CDS             complement(3541..3918)
                     /locus_tag="AC159B_02045"
                     /inference="COORDINATES: protein motif:HMM:NF018257.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HIR3376567.1"
                     /translation="MKGRILVTLLVALTATACGPGGPPGGGPQAGPPGPPGSVPVSEK
                     EWTITFPTTAIKAGRLTFRVKNEGAIEHNFVIEAARLQIDAIQPGQTKEASADLRAGT
                     YEVLCNIPGHKEAGMKATLTIAQ"
     gene            complement(4068..4511)
                     /locus_tag="AC159B_02050"
     CDS             complement(4068..4511)
                     /locus_tag="AC159B_02050"
                     /inference="COORDINATES: protein motif:HMM:NF014692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HIR3376568.1"
                     /translation="MTSPKIVGFICDWSIDIAPLLSGNRLRDLPNVYIIRVPCSGFVR
                     PAWLEDALNQGADGVFVTGCPYGDCLNREGNFLMRDRVDQLQRRLQRRRIDPARMAML
                     AHGLHDRTEFLADVQAFSDRLSEIATARAKPAASAEPAPAPGGSS"
     gene            complement(4508..5701)
                     /locus_tag="AC159B_02055"
     CDS             complement(4508..5701)
                     /locus_tag="AC159B_02055"
                     /inference="COORDINATES: protein motif:HMM:NF012263.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HIR3376569.1"
                     /translation="MSTVTSSGRYGRVMTSLLRVTLRLDTFINKIYPTDFNPLYYTGG
                     LSNLFLFVLVLSGIFLFFYYEASLGAAFESVRYISDVVPYGGIIRGVHRYSADGFIIG
                     VLLHLFRNWFTDRHMFSRDNPWISGMFLLLFGGFIGFTGYQLVWDERAQLLTSMFVAM
                     LRSIPAVGDPLARVFIGGEGIGEGTLVRVLFLHIVPASTLYAMLWWHYLRLRHPKIWP
                     PGVWILFVVGIVFLLAGVIPAISGQPAAPSSRPTSFPLDVFFMLPFWLLNWLSPGAVV
                     FLSVLLFAAGLAVPYFSRRETPAHMGVRHAGVAQVIDGNCTGCELCYFDCPYNAIIMV
                     PSPEPGLSKAAANRTLLAVVLESRCVECGICIGACPFEALELPKLMERDVQSQIANAV
                     QPAQA"
     gene            complement(5698..5898)
                     /locus_tag="AC159B_02060"
     CDS             complement(5698..5898)
                     /locus_tag="AC159B_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376570.1"
                     /translation="MRAMHGIGIVLLSGLALVFAAGIIIGEATDQWYFGGALVGALLL
                     AYSFIILLMRKMGIIGPRKAER"
     gene            complement(5920..6162)
                     /locus_tag="AC159B_02065"
     CDS             complement(5920..6162)
                     /locus_tag="AC159B_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376571.1"
                     /translation="MRAFGYLVAFAVAIWSLSAGYASLRETWQAPQPAGIITTLLYAS
                     LFVAAFLYLGYWSYAAERAAGRVRRRIGLYEWFLRG"
     gene            complement(6159..7307)
                     /locus_tag="AC159B_02070"
     CDS             complement(6159..7307)
                     /locus_tag="AC159B_02070"
                     /inference="COORDINATES: protein motif:HMM:NF012263.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HIR3376572.1"
                     /translation="MVADLAGRTRDWFRERREKLDLFDETLVGKQDNPIYLMGPLLYY
                     FWLLTVVTGLVLMIWYEPTTTGAYSSIERIQHDVPLGWLIRGLHKYGADALITVIFLR
                     VWRMYFLAEYKKPGELSWMLAFLGLILGMISGITGYLLIWNQRAFWAAKVVLTTPVYF
                     DEIPIIGGLKFGSMIAYVFLGGPAIGQATITRFYALHFGISLVLLILVEVLFFRTRRK
                     RMNMSLLPIVLFTAMLIVISYILPAESGRRADPTRTPLPILSDWYFLALYQYVKYTPP
                     LWAGLGPGLLINFGMLVPFLDRSKGRRPLERPFFFVAGVLAVVYFLVFTALIMFNIAV
                     IDRDPFLIMVFTLAVLSAGFVWELRYRRKQAQRPSVTPAPARPASAHT"
BASE COUNT         1584 a         2351 c         2220 g         1259 t
ORIGIN      
        1 ggccaaatgc cagcacaaac gcggtggcga atgacgtgta cgcggcgacc gagatcatcg
       61 gaaccaggtc gggcgtctgg taactgagca gaaagcgtac gccgacggga aggatggcaa
      121 ggaacgcaaa cagcgcgccg gcgacgaaga gcatcagcga aaaaggcagc agcgagagcg
      181 tataccggcg ttccgtccgc gtcaggccga ctgagacaaa cagccagatc tgaaccagta
      241 tcacgggaag accgaggaat actccggcga gggccgcgac cttcagacgg acgaagaatg
      301 cttctgtcgg cgcgaggaac acgacgtgcc cgatgggccg gatgagcgcc gccagaatac
      361 gatccacaaa cagccaactc agcaccatgc cgatgaaaaa tgcggcgatc gagatgagca
      421 gccgtctccg cagctcctcc agatgctcga tcaacgtcat gggccggtct tccatagctt
      481 caccaaaagc gttgaaggtt gaatgttgaa ggttgaaggt ccccaacgat tcgcgctggg
      541 gttgtcacct tcaaccttta accttcaacc agtgcttagt gtcaggaacg cttgtcgggg
      601 gttgtcgact tgtcgatctc gttctcggcg tcgcgcacgg cgccgcggaa ctccctgatg
      661 gcgcgtccca ttgcggagcc gataccggcg agccgctgcg gtccgaagat caggagagca
      721 atgacgagga tgacgatcaa ctcgggccag ccgatattgg gcatcagcca cctccacgtc
      781 caattataac ttgctccgtc cccttggacg aataatcggc tgtccgcgtt tgggctctgc
      841 gacgggacac cgcccagact cctgcggcga tacccgccac cgcgccggcc acccatgccg
      901 gcacggaggg cgccgcctca cgagcgattg ccgctggatt gcccctgctc actacagctc
      961 tcagcgcccg cagatcttgg ctaatatcca ttctaagctt ctccgcacgt tctctaggat
     1021 cggatccgga aagccggcca gcggacatcg gttctctacg cctcccgcgt cagcgactcg
     1081 cgcatggtga tgaagtcttg ccccagatct cggatgacct gaaacccttt gcgccggaat
     1141 ttgctccggg cgaccagcag caatacgccg gccacggcga gcatggccgc aaagacgatc
     1201 cccgtggcgc cccagggcgg catgacttct gcgagcagga ggatgacggt taccacggcg
     1261 agcgggacgg acagtgccag cagcacgatt gcgacgacga ccaggacagc cccggtaatg
     1321 agaccgcgga cgacggcctg tccttctcgc tgcgcctgct ccaatcgagc acgaacgagc
     1381 gccacgatat tgtgcagaat ccgattgacc cgcgttcctc tcacttccgg tcccttctta
     1441 tcttccactt gccactcctc tcaagctgaa aagctggaaa gctgaagggc tgaaggagta
     1501 cttttccggt tctccagctc ttcagctctt cagctcttca gcctcactga gcagcgcatc
     1561 tcgtagtgac acggcgaacg gccggagcga gagccggaac gtttggacgg cgttcgtatt
     1621 atcgcacaca ttattgacca gcagggtctc aaactcgtcc ggcgtgatcg gcgggttcgg
     1681 cagcaggtgg aagatgcgga ctatgatgga cgccacgccg cgtggaatgt gaaggttcca
     1741 tcgccgcagg cggaggacct ccgtgaccgt gtccatgatc tgatcatagc taacggcgtc
     1801 cgggccgcct aactcgaagg tttgccctag aatgtcgtcc cgctcgagcg cctgaaggac
     1861 agcggtgaag agatcgcgat gatcgatcgg catgaagcgc gtctgccccg atccgagaat
     1921 ggggatgacc ggcgtaaggt tggcaaatgc cgcaagcacg cggtcgcggc ccagcattag
     1981 tcgcagcggg taccagggcc cgaaccgaac ggcctgggcg aactgctcgg cagcgccgcc
     2041 gcccggcccc ataataaagg aaggcctgaa gatgacctgc ctgacgccgg agtcccggac
     2101 ttcctgttca cccgcccact tcgagcgcag atagcgtgtc gcggacgggc cggccccaag
     2161 cacgctgaag tgaatgagtc gatccacgcc ggcggtcttg gccgcgtcgg cgacgttgcc
     2221 cgcgccctgc ctgttgatgc tgtcgtatgt ctcgccggcc gattctctaa ggatcgccac
     2281 cagatggact gcggcgcggc atccggccag cccggcatcg agtgaccgtc gatcccgaac
     2341 gtcccccagg gccacctgat gcccgagcgc gcgcagacgg tcggccgcga cggccgacgt
     2401 gacgattgct ctgacggtcc agccggcacc ggccagcgcc ctcgtgagca gcgatcccac
     2461 aaatccagac gcgcctgtca ctgccacgac gccctgcggc atcaacgcgt ccggacaacg
     2521 acaaactcgg caagacgcac caggctgtcc ttggccgccc catctggcag cggggtaagc
     2581 gcggtaaccg cttggcgaga atatcgcgcc gccacgtcga gcgcatagtc aattccgtga
     2641 tagcggcgca acagctggaa cagttcctca cgctgtccat ctcgcgcggg gccgctgagc
     2701 aggtggacca aatggtcgcg gtcttccggg gttgcgtcac gaagcgctcg aatgaccggc
     2761 agcgtgactt tgcctccctc aatgtcgctg tggatgggct tgccgagaga ttcctcccgg
     2821 ctggtcaggt cgagtgcgtc gtcggtgatc tgaaacgcca tgccccactc cagcccgtac
     2881 gtgctcaaga gggcggcggc atgcgcattt ccggcgacga gcgccccggc cttacacgcg
     2941 gcggaaaaca gatgcgcggt ctttccggca atgatccgga agtagatgtc ctcgtcgtcg
     3001 tgaggctgct gaccgtactt gatctgcatg atctcagcct ggctcatctt gaccgtcgcc
     3061 agggccatcg tcggcgccac ctcagggtgg cggacgcgac tcaacagatc aaacgccttg
     3121 gcaaacaagt aatccccgag gaggaccgcg atctggttgc cccactgcac gtttacggtc
     3181 ggctcgccgc gccgcagatc agcgtcgtcg atgatgtcat cgtggatcaa cgacgcgatg
     3241 tgaatcagct ccaccgcggc ggcgatccac aaccgcgcat ctccggctcc gccactggcc
     3301 tgcgccgaaa ggcacaccag tgcgggtcga agcatcttcc cgcgcgtctg cagcacgtgg
     3361 cgcacgagtt cggcaatgaa gggatcgtcc gagcccagct cctgctccat gacagactga
     3421 agcctctcca aatcgtgctg cacgggtaca tacaagtgtg aaagatccat cacgctcgtc
     3481 ttcgataaga aaagacggcg gcctcccagc cgccgtcttc gtgaccgccg ccccagtttt
     3541 ttattgtgcg atggtgagcg ttgccttcat ccccgcttcc ttgtggcccg ggatgttgca
     3601 caggacctcg taggttcccg ccctgaggtc ggctgaggct tccttcgtct gccccggctg
     3661 aatagcatcg atctgcagcc gcgccgcttc gatcacgaag ttgtgctcaa tcgccccttc
     3721 gttcttcacg cgaaacgtaa gtctgcccgc tttgatggct gtcgtcggga atgtaatggt
     3781 ccactctttc tccgacactg gcacggagcc tggaggaccc ggcggcccgg cctgcggccc
     3841 accgcccgga gggccacccg gaccgcacgc agtggcagtg agagccacga gcagcgtcac
     3901 caagatccgt cccttcacga catccctcct gtcaagttac tactcaccat tcacgattca
     3961 ccatcaggct tcagcaatca atccgaacga cgcgatgatg gtcagcaaga tgaaccagag
     4021 gaggtagaag atcagatagg caagatgcac ccggagcggc cgtttcttca cgacgatcct
     4081 cccggagcgg gggcaggctc tgccgaggca gcgggtttcg ctcttgcggt ggcgatctcc
     4141 gaaaggcgat ctgaaaaggc ctgcacgtcg gcgaggaatt ccgttcgatc gtgcaagcca
     4201 tgcgcgagca tggccatccg ggcgggatcg atcctccgcc gctggagccg tcgttgcagc
     4261 tgatcgacac gatcccgcat cagaaagttg ccttcacgat tcagacaatc cccatacgga
     4321 catccggtca caaagacgcc gtctgcgcct tgattcagtg cgtcctccag ccaggcgggc
     4381 cggacgaatc ccgagcacgg cacgcggatg atgtatacgt ttggcaggtc gcgcaggcgg
     4441 tttccggaaa gcagcggcgc gatgtcgatg ctccagtcgc agatgaaccc cacgattttg
     4501 ggggaagtca tgcctgagcc ggttgcacgg cgttcgcgat ctgtgactgc acgtcacgtt
     4561 ccatgagctt cggcaattcg agtgcctcaa acgggcacgc cccgatgcag atcccacatt
     4621 caacgcagcg ggactcgaga acgactgcga gcagcgtacg gttcgctgcg gctttgctca
     4681 ggcctggctc gggcgagggc accatgatga tcgcgttgta cggacagtcg aagtagcaga
     4741 gctcacaacc cgtgcagttg ccgtcgatga cctgcgccac ccccgcgtgg cgcaccccca
     4801 tgtgcgccgg cgtctcgcgg cggctgaaat acggcacggc cagcccggcg gcgaataaca
     4861 ggacgctgag gaagaccacc gcgcctgggc tcaaccagtt cagcagccag aacggcagca
     4921 tgaagaatac gtccaacgga aatgatgtcg gccgcgacga cggcgccgca ggttgcccac
     4981 tgatcgccgg gatgaccccg gccaaaagga agacgatgcc gaccacaaag aggatccaga
     5041 ctccgggcgg ccagatcttt ggatggcgca accggagata gtgccaccac agcatcgcat
     5101 acagcgtcga ggcaggaacg atgtgcaaga aaaggacccg aacgagggtt ccctcgccga
     5161 tgccctcgcc tcctatgaag actctggcga gaggatcgcc gacggccgga atgctccgca
     5221 gcatcgcgac gaacatcgag gtgagcagct gggcgcgctc gtcccatacg agctgatagc
     5281 cggtaaaccc gataaagccg ccaaacagca agaggaacat tccggagatc cagggattgt
     5341 cacgtgaaaa catatggcgg tccgtaaacc agttcctgaa caagtgcagc aggacgccga
     5401 tgatgaaacc atcggccgaa taccggtgga cgccgcggat gatcccgccg tagggtacga
     5461 cgtcgctgat gtatcggaca gactcgaagg cggcgcccag gctggcctca tagtagaaga
     5521 agaggaagat accggagaga acgagcacga acagaaacag gttgctcagc ccgccggtgt
     5581 agtacaacgg gttgaaatcg gttggataga ttttgttgat gaaggtgtcc agccgcagcg
     5641 tcacgcgcaa caggctggtc atcacgcgcc cgtaccggcc gctcgaagtc acggtgctca
     5701 tcgctcggcc ttcctcggtc cgatgatccc catcttccgc atcaacagga tgatgaacga
     5761 ataggcaagc agcaatgcac ccaccagagc gccgccaaag taccattggt cggtggcttc
     5821 gccgatgatg ataccggctg cgaagaccag tgccagtccc gacagcagca cgattcctat
     5881 tccgtgcatc gcgcgcacag tgcgctccct aggtgttgat tagccgcgaa ggaaccactc
     5941 gtacaacccg atacgccggc gcacccgccc cgccgcccgc tccgcggcgt agctccaata
     6001 gcccaggtac agaaacgcgg ccacaaagag gctggcgtat aacagggtgg tgatgatccc
     6061 cgcaggctga ggagcctgcc aggtctcccg gagcgacgca tatcccgccg acaacgacca
     6121 gatggctaca gcaaacgcca cgaggtaccc aaatgccctc atgtgtgcgc gctcgcaggg
     6181 cgcgccggcg ccggcgtgac gctcggacgc tgggcctgct tcctgcggta ccgcagctcc
     6241 cacacgaatc cggccgagag gaccgccaat gtgaacacca tgatgagaaa tgggtcacgg
     6301 tcgatgacgg cgatgttgaa catgatcagc gccgtgaaga ccaggaagta cacaaccgcc
     6361 agaacgcccg caacgaagaa gaacggccgc tccagcgggc gccggccttt gctgcggtcc
     6421 aggaacggca ccagcatgcc gaagttgatg agcagccccg gacctagccc cgcccacaat
     6481 ggcggcgtgt acttcacata ttggtagagc gccaggaagt accagtccga aagaatggga
     6541 agcggggtcc tggtgggatc ggcgcggcga ccggactccg ccggcaagat gtacgagatc
     6601 acaatgagca ttgccgtaaa gagcacaatc ggtagcaggg acatgttcat ccgcttgcgc
     6661 cgcgtgcgga aaaacaggac ttcgacaaga atgagcaaca ccaacgaaat cccaaagtgg
     6721 agcgcgtaga atcgcgtgat ggtcgcctgc ccgatcgcgg ggccgccgag aaacacatac
     6781 gcgatcatcg aaccgaactt caaaccaccg ataatgggga tctcgtcgaa gtagaccggt
     6841 gtagtcagca ccactttggc cgcccaaaac gcccgctggt tccagatcaa gaggtaaccg
     6901 gtgatccccg agatcattcc caggatcagc ccgaggaacg cgagcatcca cgagagttcg
     6961 cccggcttct tgtattccgc caggaaatac atccgccaga cccgcaggaa gatcacggtg
     7021 atgagcgcat cggcgccgta cttgtggagg ccccggatca accagcccag cgggacgtcg
     7081 tgctggattc gctcgatgct gctgtacgca cccgtcgtcg tcggctcgta ccagatcatg
     7141 agcaccaaac cggtcacgac cgtgagcagc cagaagtaat acagcagcgg ccccatcagg
     7201 tagatgggat tgtcctgctt gccgacgagc gtctcgtcga agagatctag tttctcgcgg
     7261 cgctcgcgga accagtccct ggttcttcca gcaagatcgg cgaccatctg ggtcaggccg
     7321 tttcccccgc gtccttcttc ttcgcaatgc ctcccggctc cacgtcggtg accacgatgt
     7381 cgtcgccctg gatctcgaac agaaaggtcc gcgg
//
LOCUS       DBNGSB010000074         6165 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2349681, whole genome shotgun sequence.
ACCESSION   DBNGSB010000074 DBNGSB010000000
VERSION     DBNGSB010000074.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 6165)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6165)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6165
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2349681"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..166)
                     /locus_tag="AC159B_02075"
     CDS             complement(<1..166)
                     /locus_tag="AC159B_02075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HIR3376573.1"
                     /translation="MNMIRKHTRGTAGFTLVEIAIVVAIIGLLLLIALPLFTGARVRA
                     YVAEARSLTSE"
     gene            complement(438..1655)
                     /locus_tag="AC159B_02080"
     CDS             complement(438..1655)
                     /locus_tag="AC159B_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR3376574.1"
                     /translation="MARFTYRAYDRTGRLEVGVMDAETPRVAAATLQSRGYLVSAVSE
                     QGQQPERAMRQLRRLKGVNRRDLVIFTRQLATMISAGLPIVTALRVLEEQASQQQLRE
                     VIGGVRAGIERGGSLSSEIAKHPQAFFEFYVNTIRAGEVSGLLDVSLEYLADYLEREL
                     DLVQRVRTAATYPLLVLIFTLMVATAAIVLIVPMFVQIFRSFKVALPLITIIMVRVSL
                     AVRSLWWLGLLALVAAGVGLFMLRRTRAGRRTLDSLLLRLPIIGQIIQKLAFARFGRS
                     MAVVIRSGIPLLEGLDAVADSLGNRVIGDAVAAARERMRQGKSMADALDRSSLISPMV
                     VQMIRVGEETGAMETVLNKIAEFYEREVDNTVKRFASIVEPVLIMLVGGVVAIVALAV
                     LLPIWTLIARLPR"
     gene            1823..3880
                     /locus_tag="AC159B_02085"
     CDS             1823..3880
                     /locus_tag="AC159B_02085"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HIR3376575.1"
                     /translation="MTGLRATRPLDQFAQSVLAVRWLVLAALIALEAVEPPRTPYEVA
                     LPLYVGILVYALGLTLLAWAFPHRAEVTTRLAVPFDLAAVLVGMQFTAHPREFFVLGF
                     VVCAMSGFVLGRVAAVLVAAAMTLLQVPGMPASLFSPERHLSWGLAALGLLLTSSVAG
                     VAGVKTRGDGGSRALAELDAALGQGRSHALDTALESLMTDLRADSGSIMLFDADASRL
                     EIVAARGLHESHREEKPRMGEGVAGWVLQEMRPVLLTPNAPVPFELSRHEIGSSLSVP
                     LLMGTRPLGVLNLNRSTGRPWFTLPDLDQASALALQVRVLLLDAQHERTLSGALEHLA
                     AGQQEVGRALSRDPTVLWPVLLDVARTVTAARFAVLALERDDTGTIDIVSARGIDAPS
                     AKQLLPDLMTASTHGNVHEVNGQQQALRTVCVPLHVQNRAIGAIALGGLPGDGRVPPA
                     LLGAVAAHIAAAVHTARTAYRIADIGVIEERRRIAREIHDGLAQTLADALLQTDLSAM
                     IAQSDPSKLDHDLRELRTVLERAMRELREFMTELRRQEEPNSGLRASLDTLSKEFQRR
                     HSTPVRLIVAGDDAHLPSAVRHAILAIARQALTNVGAHAHAATVTIRVEISEQHCAAS
                     ITDDGVGFDLATYRSRPPAAHHLGLTSMEERAALVGGRLQLETAPGHGTTVNVTVPMG
                     RHA"
     gene            3873..4571
                     /locus_tag="AC159B_02090"
     CDS             3873..4571
                     /locus_tag="AC159B_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3376576.1"
                     /translation="MPNPHGPAGGRGGPIRVLLADDHDLFRQGVRRLLEATEDVEVIG
                     EAPSGEAAVRLVEDLAPDVVLLDITMPGLSGIDAARIVKATSPRTSLIMLTVHGEEEF
                     LFEAIKAGAMGYLLKDCTAEELLRAIRVVYEGEGLLAPTMAAKVMHEFARTRETHELA
                     AVTHPLTQREVEILQHVASGLANKEIALRLKISERTVKNHLSNIMEKLHVNSRTQAAV
                     YALRSGLVPPEQKR"
     gene            4623..5018
                     /locus_tag="AC159B_02095"
     CDS             4623..5018
                     /locus_tag="AC159B_02095"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3376577.1"
                     /translation="MPGNDRPSISQTRVLLVDDNPGFRQRMARLLELQTDLAVVGEAS
                     DGNEAITRVRELHPDVVLMDFRMTGLDGFTAARTITAEFPKVKVFMLTAFPGALDPDK
                     VAKNGIAGLLIKDQPASEILAAIRKSVTR"
     gene            complement(5008..>6165)
                     /locus_tag="AC159B_02100"
     CDS             complement(5008..>6165)
                     /locus_tag="AC159B_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012652.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HIR3376578.1"
                     /translation="INMLITQAMKDRASDIHIEPTEEDVKVRFRIDGVMLAVMNVPKT
                     AHPALLSRLKVLARLNIAEKRIPQDGAFEMAVDGHTVDFRVATIPTIFGERAALRLLD
                     KSKGVLTLEQLGMDAETRRRYSSLAGQPYGIILVSGPTGSGKTTTLVSTLAQLNSADK
                     NIMTIEDPVEYQLPGVSHIQINPRAGLTFATGLRSIVRQDPDIIMIGEVRDVETAEIA
                     IGASLTGHLVLTTIHTNDAPSALARLTDMGIEQYLIASAVMGVVSQRLVRLLCPHCRK
                     PEPAPADIIEWLQAMVKDPLPEPKMFHPQGCPRCKDSGYLGRSGIFEVLSMTEPIRKL
                     VLARASAAELTDVASRDGMASMRVDGLLKAIRGLTSVEEVLRVTRVEEPNA"
BASE COUNT         1107 a         1995 c         1940 g         1123 t
ORIGIN      
        1 attcggacgt gagcgaacgc gcctctgcaa cgtaggcccg cacccgcgcg ccggtgaaga
       61 gcggcaatgc gatgagtagg agcaatccga tgatcgccac gacgatggcg atctcaacga
      121 gggtgaagcc cgcggttcct ctagtgtgtt tgcgaatcat attcatgaac gtccagcctc
      181 ccgttgccta ttgcccccgc ccttgccccc gcagtcgagg gtcttcacgc tacatcttca
      241 agcacgcggc atgcctcaac aatgggaagt tcggttggcg cgcgtagtcc caaagtacta
      301 cccggcaaac atcccagttg ggcctgcttt cggggaggtc gcagcctctc cccgactgca
      361 actgatacgg ggacccctca ggctacgacc tccccggcgc aggcccgatg ctagtgcaag
      421 aggatgcgcg ctgcatttta tcgaggtaga cgcgcgatga gggtccaaat gggcagcagc
      481 actgcgagag cgacgatcgc cacgactccg ccgacgagca tgatgaggac cggctcgacg
      541 atggaggcga accgcttcac cgtgttatcg acttcccgct cgtagaactc cgcgatcttg
      601 ttcaacaccg tctccattgc gcccgtctcc tcgcccacgc ggatcatctg gacgaccata
      661 ggagagatga gggagctgcg gtcgagcgcg tcggccatcg atttcccctg ccgcatccgc
      721 tctcgtgccg ccgccaccgc atcaccgatc acccggtttc caagcgagtc cgcgacggcg
      781 tccaatcctt cgagcagcgg aatgccgctg cggatgacga cggccatcga gcggccaaac
      841 cgggcgaagg cgagcttctg aatgatctga ccgatgatcg ggagccgcag cagcagggag
      901 tccagggtac gtctgccggc ccgggtgcga cggagcatga agaggccgac gccggccgcg
      961 accagtgcaa gcagcccgag ccaccacagg gagcggaccg ccaggctcac ccgcaccata
     1021 atgatcgtga tcagcggcaa ggcgacctta aaagaccgaa agatctgtac gaacatcggc
     1081 acgatgagaa ctattgccgc ggtggccacc atcaacgtga atatcagtac cagaagcggg
     1141 tacgtcgcgg ccgtccgcac tcgctgaacc aggtcgagct cccgctcgag atagtccgcc
     1201 aggtactcca gtgagacatc gaggaggccg gagacttcgc cggcccggat ggtattcacg
     1261 tagaattcaa agaacgcctg ggggtgcttc gcgatttcgc tgctcaggct gccgccgcgc
     1321 tcaatcccgg cgcgcacgcc gccgatcact tcccgcagtt gctgctggga cgcctgttcc
     1381 tctagaacgc gcagcgctgt gacgataggc aggcccgcgg agatcatcgt ggctagctgc
     1441 cgcgtaaaga tgacaagatc gcgcctgttg acccctttga ggcggcgcag ttgccgcatc
     1501 gcgcgctcgg gctgctgccc ctgttcgctg accgcgctga cgaggtagcc gcgagactgc
     1561 agggtcgccg cggccacgcg cggcgtctcc gcgtccatca cgccgacctc gagccggccc
     1621 gtgcgatcgt aggcccggta tgtaaatctc gccatcgtgg cacatccgaa gcagtgtcgg
     1681 gctaagtgta cgcaaggaat tccttgcccg gaaatggtcc tttccgacta cccgcccctc
     1741 cctcaatcgg aaaagggtcg ggtgaagggg gaagtgtttt gcatcgccgc gaacagcaag
     1801 gaaccttggg aggacctgct tcgtgaccgg acttcgcgca acgcgaccgc tggatcagtt
     1861 cgcacaatcc gtcctggcgg tccggtggct cgtgctggcc gcgttgatcg cgctcgaagc
     1921 ggtagagccg ccgcgcactc cttatgaggt cgcccttccg ctttatgtag gcatcctcgt
     1981 ctacgcgctg ggtctcacgc tcttggcctg ggcctttccc catcgcgcgg aggttacgac
     2041 gcgcctggcg gtgccgttcg atctcgccgc agtcctggtg ggcatgcagt tcaccgccca
     2101 cccgcgcgaa tttttcgtcc tgggcttcgt cgtctgcgcc atgtcgggat tcgtgctcgg
     2161 ccgggttgcc gcagtgttgg tggcagcggc gatgacactt ctgcaggtcc cggggatgcc
     2221 ggcttcattg ttcagtccgg agcgtcatct ctcgtggggc cttgccgcgc tcggattgtt
     2281 gctcacctcc tcggtcgccg gcgttgcggg ggtgaaaaca cggggcgacg gtggctcacg
     2341 tgcgcttgcc gagcttgacg cagcgctggg ccagggccgc tcacatgcgc tcgacacagc
     2401 actggaatcc ctcatgacgg atttgcgcgc agacagcggg tccatcatgc tgtttgatgc
     2461 cgatgcctca cggctggaaa ttgtcgccgc ccgcgggctc cacgagtcgc accgggagga
     2521 aaagccgcgg atgggcgaag gggttgcggg ctgggtactg caggagatgc gtccggtgct
     2581 gttgactccc aacgcgccgg tgcccttcga actgtcccga catgagatcg gctcgtcgct
     2641 aagcgtgccg ctgctgatgg gcacgcggcc gcttggggtc ttgaacctga accgatcgac
     2701 gggtcggccg tggttcacac ttcccgatct cgaccaggcg tctgcactcg cactgcaggt
     2761 tcgtgtcctg ctgctcgatg cgcagcatga gcgcacactg tcaggagcgc tcgagcatct
     2821 tgccgccggt cagcaggaag tcggccgcgc cctttcgcga gacccgacgg tgctgtggcc
     2881 ggtcctgctg gacgtcgccc gcacggtgac ggcggcacgg ttcgcggtgc tggcgctgga
     2941 acgcgacgac acgggcacga tcgatattgt ctccgcgcgc gggatcgacg cgccttccgc
     3001 caaacaactg ctcccggacc tcatgacggc cagcactcac gggaacgttc acgaggtcaa
     3061 cggacagcaa caggcactcc gcacggtgtg cgtgccgctc catgtgcaga atcgcgcgat
     3121 cggcgctatc gcgctggggg gcctgccggg tgacggtcgc gttccgccgg cgctgctcgg
     3181 cgccgtcgcc gcgcacatcg ccgccgccgt ccacaccgcc cgtacggcgt accggatcgc
     3241 agacatcggc gtgatcgaag aacgccggcg catcgcacgg gaaatccacg acggcctggc
     3301 gcagacgctt gccgacgcgc tcctgcagac cgacctctcc gccatgattg cccaatcgga
     3361 cccgagcaaa ctcgatcacg atctgcggga gctgcgcaca gttcttgagc gagcgatgcg
     3421 ggagctgcgg gagttcatga cggagttgcg ccgccaggaa gaacccaaca gcgggctgcg
     3481 cgcgtccctc gataccctca gtaaagaatt tcagcggcgt cactcgaccc ccgtgaggct
     3541 gatcgtcgcc ggcgacgacg cgcacctgcc atcagccgtg cgtcacgcca tcctggccat
     3601 cgcccgtcag gcgctgacca atgtcggggc gcatgcccac gccgcgaccg tcaccattcg
     3661 cgtcgagatc agcgaacagc actgcgccgc ttcgattacg gacgatggtg tggggttcga
     3721 tctggccacc taccgcagca ggccaccggc tgcgcatcac ctcggactga catcgatgga
     3781 ggagcgcgcg gcgcttgttg gcgggcgtct gcagctcgag actgccccgg gccacgggac
     3841 gacggtgaat gtcaccgtcc caatggggcg ccatgcctaa cccacatggg ccggcgggag
     3901 gacgaggtgg accgattcgc gtgctgctgg cggacgacca cgatctgttc cggcaggggg
     3961 tgcgtcgcct gctcgaggcc acggaagacg tcgaggtcat cggcgaagct ccgagcgggg
     4021 aagcagctgt ccgcttggtg gaagacctcg cacccgacgt ggtcctattg gatatcacca
     4081 tgcccggtct ttccgggatc gatgccgcgc ggatcgtcaa agccacgagt ccccgcacaa
     4141 gtctgatcat gcttacggtg catggggagg aagagtttct gttcgaggcc atcaaggccg
     4201 gcgcaatggg atacttgctc aaggactgca ccgcggaaga actcttgcgg gcgatccgcg
     4261 tggtctatga gggcgaagga ctcctggcgc ccacgatggc cgccaaagtc atgcatgagt
     4321 tcgccagaac gcgcgaaacg cacgaactgg ccgcggtgac ccaccccctc acgcagcgcg
     4381 aggttgagat cctccagcac gtcgcctcgg gactcgccaa caaagagatc gcgttgcgtc
     4441 tcaagatcag cgaacgcact gtgaagaacc acctcagcaa catcatggag aagttacacg
     4501 tcaacagcag aacacaggcc gcggtgtacg cgcttcggag tgggctcgtg ccaccagaac
     4561 aaaaacgcta gtacctacag accatttacg gttcgaatca accgggggta gacttctttc
     4621 gtatgccggg aaacgacaga ccttccatct ctcagactcg tgtcctcctt gtcgatgaca
     4681 acccggggtt ccggcagcgt atggcgcgcc tgctcgaact gcagaccgac ctggccgtcg
     4741 tgggcgaagc gtcggatggc aacgaggcga tcacccgcgt ccgtgagctc catcccgacg
     4801 tcgtcctcat ggacttccgc atgaccggcc tcgacggatt cactgccgcg cggacgatca
     4861 ccgcggagtt tcccaaagtc aaagttttca tgctcacggc gttccccggt gcgctggatc
     4921 cagacaaggt tgcgaagaac gggatcgcgg gtctgttgat taaggatcag ccggcctcag
     4981 agatcctggc agcgattcga aagagcgtta cgcgttaggc tcttccaccc gcgtcacccg
     5041 caggacttcc tccacgctcg tcaacccccg gatcgctttc aacagaccgt ccactctcat
     5101 gctggccatg ccatcccggc tcgcaacgtc ggtgagctcg gccgccgacg cccgcgcgag
     5161 gacgagtttg cggattggtt ctgtcatcga gagcacttcg aagatcccgg aacggccgag
     5221 atacccactg tccttgcagc gcgggcagcc ctgcggatgg aacatcttcg gctccggcag
     5281 tggatccttt accatggcct gcagccactc gatgatgtcc gccggcgccg gctctggctt
     5341 ccggcagtgg gggcatagca agcgtacgag acgctgcgac accacgccca tcacggcaga
     5401 agcgatcaag tactgttcga tgcccatgtc ggtgagccgc gccagggcgc tcggtgcatc
     5461 gtttgtgtga atggtggtca agaccaggtg tccggtgagc gaggcgccga tcgcgatctc
     5521 ggcggtttcc acgtctctga cttcgccgat cataatgatg tcggggtcct gacggacgat
     5581 cgagcgcagc cccgtcgcga acgtcaggcc cgcgcgcggg ttgatctgga tatggctgac
     5641 gcccggaagt tgatactcca cgggatcctc aatcgtcatg atgttcttgt cggcagagtt
     5701 gagctgcgcg agcgtgctga ccagggtggt cgttttcccg ctgccggttg gtccgctcac
     5761 gaggatgatc ccgtacggtt gcccggccag gctcgagtag cggcgccttg tttccgcatc
     5821 catcccgagc tgctcgaggg tgaggacgcc tttcgatttg tccagcagcc gcagcgcggc
     5881 gcgttcacca aagatcgtcg gaatcgtcgc aacccggaag tcgacggtgt gtccatcgac
     5941 agccatctca aacgcgccgt cttgtgggat ccgtttctcg gcgatgttga ggcgggccag
     6001 gaccttcaat cgcgacagca gcgccgggtg ggccgtcttc ggcacgttca tgactgccag
     6061 catgacgccg tcgatgcgga accggacttt gacgtcctct tccgtaggct cgatatgaat
     6121 gtcgctcgcg cggtccttca tcgcctgcgt gatcagcatg ttgat
//
LOCUS       DBNGSB010000075         3604 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_970440, whole genome shotgun sequence.
ACCESSION   DBNGSB010000075 DBNGSB010000000
VERSION     DBNGSB010000075.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_970440"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="AC159B_02105"
     CDS             complement(<1..285)
                     /locus_tag="AC159B_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3376579.1"
                     /translation="MDSVITQRAGRREWIGLAVLALPCILYAMDLTVLNLAVPSLSAD
                     LKPTSAQLLWIIDIYGFLVAGSLITMGTLGDRIGRRRLLLTGAVLFAITSV"
     gene            complement(515..997)
                     /locus_tag="AC159B_02110"
     CDS             complement(515..997)
                     /locus_tag="AC159B_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020473016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3376580.1"
                     /translation="MVTSTSEQAIHTLEFTKDVQIAAPVEIVFETLLEHLGPLHETAP
                     DYPLPMKLEPWPGGRWYRDLGNNAGHLWGHVQVIKPPKLLEIHGPLFMSYPAIAHMQY
                     RLTEEGDVTRIRLIHGAIGRIPPEQREALAEGMEPWSHTLMKVREAAERRNGAFGGGR
                     "
     gene            complement(1009..1350)
                     /locus_tag="AC159B_02115"
     CDS             complement(1009..1350)
                     /locus_tag="AC159B_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020473015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR3376581.1"
                     /translation="MARAATTTDVFNAIAEPRRREIIRLLGGGRAHAVGELVKRLRLP
                     QPAVSKHLGVLRKVGIVSVNRHGRHRLYRLNAEKLKPVHDWAKTFERYWTHQLDRIKE
                     RAERKARERLD"
     gene            complement(1476..2744)
                     /locus_tag="AC159B_02120"
     CDS             complement(1476..2744)
                     /locus_tag="AC159B_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002627383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3376582.1"
                     /translation="MTVSTTHRTVDAIWRIESAKVIAGIARIVRDVGVAEDIAQDTLV
                     AALEQWPESGVPDRPGAWLMGTAKHKAVDFLRRKMLQDRKHQELGRELEVEREAAASG
                     FDRALDEEIDDNLLRLIFTACHTVLSPEARVALTLRLLGGLTTEEIARAFLVPMPTIA
                     QRIVRAKRTLAETRVPFEVPSGAELAPRLSSVLEAIYLIFNEGYAATAGDEWVRPALC
                     EEALRLGRILAELVPRESEVHGLVALMEIQASRLRARRGHAGEPILLFDQDRGRWDYV
                     LISRGLAALERAEKLGGTLGPYTLQAAIAACHARARTPAETDWSRIVALYDALGQLAP
                     SPVVELNRAVAVAMAFGPAAGLELVDDLTSEPSLKNYHLLPSVRGDLLAKLGRFDEAR
                     TEFERAAALTQNTRERTLLLDRAASTGIPE"
     gene            complement(2769..3242)
                     /locus_tag="AC159B_02125"
     CDS             complement(2769..3242)
                     /locus_tag="AC159B_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013937856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HIR3376583.1"
                     /translation="MRVMVLMPADKNSEKGILPDEKLIAGMVKYNEDLVKAGVMLAGE
                     GLHPSSKGVRVRLSPTGKRTVIDGPFTEAKELIAGYWLWQVKSLEEAIEWVKRLPDYL
                     ERAGWTPPKDEGDYDIEVRPVFETEEFAAELSPELREQVTEQETRIREAAAKAKS"
     gene            complement(3239..>3604)
                     /locus_tag="AC159B_02130"
     CDS             complement(3239..>3604)
                     /locus_tag="AC159B_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3376584.1"
                     /translation="VLVVEFTLMGQSFSGLNGGPYFKFNEAVSFTIPCEDQSEVDRYW
                     KALSAVPESEQCGWVKDRFGLSWQIVPTMLQRLLADPDRTKAGRVMEAMLKMKKLDIA
                     ALERAAESEPKQAHKEVRR"
BASE COUNT          661 a         1140 c         1046 g          757 t
ORIGIN      
        1 cacggacgtg atggcgaaca agacggcgcc tgtcagcaac agccgccgcc ggccgatccg
       61 atctcccagc gtccccatgg tgatgagcga tccggcaacc aggaacccgt aaatgtcaat
      121 gatccacagc agttgggcac tggtgggctt gagatcggcg ctaagggacg gaactgcgag
      181 gttcaggacg gtgaggtcca tggcgtagag gatgcacgga agcgcaagca cggccagccc
      241 gatccactcc cgacgtccgg ctcgctgtgt gataactgaa tccatgaatg cttctcctgg
      301 aattgagtcc ttcgtgtgaa tactatggcg taaggcgatc ctcgaggtcg tccgccaaag
      361 gtctgagatg gtgacaaatc atcagttgcg aggttttaat gaaggaaccg aacgggagtc
      421 gaacccgtcg ctcccggcta agctgtcgct tccagggtgc gcccctacac tacggtccct
      481 tctctcacat ccaattgatc acgaccgaac cgttttaacg gcctccgccg aacgcgccgt
      541 ttcgacgctc cgcagcttca cgaaccttca tgagggtgtg gctccaaggc tccatccctt
      601 ccgcgagggc ttcccgctgt tcgggtggaa tccgcccgat cgcgccatgt atcaacctga
      661 tgcgggtgac atcgccttcc tccgtcagcc ggtactgcat atgtgcgatt gcgggatacg
      721 acatgaacag cggtccgtgg atttctaaga gcttgggcgg cttgatgacc tgtacgtgcc
      781 cccacaagtg tcctgcgttg ttccccaggt cccggtacca gcggcctcct ggccacggtt
      841 cgagcttcat gggcaatggg taatctggtg ccgtttcgtg gagcggaccg agatgctcca
      901 gcagcgtctc gaacacaatc tcgaccggcg ctgcgatctg cacatccttc gtaaactcga
      961 gcgtgtgaat tgcttgttct gaagtgcttg tcaccatggt cactcctcct agtccaatcg
     1021 ttctcttgct ttacgctctg cccgctcctt tattcggtca agctggtgag tccagtagcg
     1081 ctcaaacgtc tttgcccagt cgtgcacggg ctttagtttc tccgcgttga gccggtacaa
     1141 acggtgccgg ccgtgcctgt tcaccgagac aatcccgact ttgcgcagca caccgaggtg
     1201 tttggacacg gcaggctgag gcaagcgaag cctcttgacc agttccccca ccgcgtgcgc
     1261 tctgccgccc ccgagcagtc tgataatctc ccgccgtcga ggctcggcta tcgcgttgaa
     1321 cacatccgtc gtagttgcgg ctctggccat cacctcgatt atattcccgt atgggaatat
     1381 gtcaagtcag gatgagaacc cgaccgaccg tttaagtgtg tcgcttttca ggttccctcg
     1441 cctccgacct tggtcggagg catgcgtgac cctgattact ctggaatgcc ggtgctggcg
     1501 gcgcgatcaa ggagcaacgt acgctcccgt gtgttttggg tgagcgcggc cgcacgctcg
     1561 aattccgtgc gggcctcgtc aaaacggccg agtttggcca ggaggtcgcc gcgcacgctc
     1621 ggcagcaagt ggtagttctt gagtgatggc tccgaagtca agtcgtcgac gagctcgagg
     1681 cccgcggccg ggccgaatgc catcgcaacc gcgaccgcgc ggttcaactc cacgacaggt
     1741 gatggagcga gttgaccgag cgcatcatag agcgccacga tacgcgacca gtcagtttcc
     1801 gctggcgtgc gcgcccgagc gtgacaggca gcgatcgcgg cctgaagtgt gtacgggccg
     1861 agcgtgccgc cgagcttttc agcacgttca agtgctgcaa gaccacgact tatcaggacg
     1921 taatcccagc gcccgcggtc ttggtcaaac agcaggatag gttctccggc gtggcctctc
     1981 cgcgctctta gacgggaggc ctggatctcc atcagcgcga ccaggccgtg gacctctgac
     2041 tcccgcggaa caagctcggc caggatgcgg ccgagacgaa gcgcttcctc gcacagcgcc
     2101 ggacgcaccc attcgtcgcc ggccgtggcc gcgtaaccct cattgaagat gaggtaaatg
     2161 gcctcgagga ctgatgaaag acgaggcgcg agctctgctc cgctcgggac ttcgaatgga
     2221 acgcgcgtct cagcaagggt tcgcttagcc cggacgatgc gctgggcaat cgttggcatg
     2281 gggacgagaa acgcgcgggc gatctcctcc gtcgtcagac cgcccagcag acggagggtg
     2341 agggccacgc gggcttccgg agacaaaacc gtatggcagg ctgtgaagat cagacgcagg
     2401 aggttgtcgt cgatttcttc gtccagtgcc ctatcgaaac ccgaggcggc cgcttctcgc
     2461 tcgacctcga gctcgcggcc gagctcttga tgcttccggt cctgcagcat cttccggcgc
     2521 aggaagtcga cggccttgtg cttagctgtg cccatgagcc aggcgcccgg tctatccggg
     2581 acgcctgact cgggccactg ctccagcgcc gcaacgagcg tgtcctgggc gatgtcttcc
     2641 gcgacaccga cgtcgcgcac gatcctcgcg atgccggcga tgaccttcgc ggactcgatc
     2701 ctccagatcg catcgacggt acgatgcgtg gtagaaactg tcaccaagcc ggttggaact
     2761 cctcagtact acgatttcgc cttcgcggcc gcctcgcgga tccgcgtctc ctgctccgtc
     2821 acctgctctc tcaactccgg gctaagttcg gctgcgaatt cctccgtctc gaacaccggg
     2881 cggacttcga tgtcgtagtc gccctcgtct ttgggtgggg tccaccccgc gcgctccaag
     2941 tagtcgggca ggcgtttgac ccactcgata gcctcttcca aggatttcac ctgccacagc
     3001 cagtacccgg cgatcaactc cttcgcctca gtgaacggcc cgtcgatcac ggtccgcttt
     3061 ccggtgggcg aaagcctgac tcgcacacct ttcgaactcg gatgaagtcc ttcacccgcg
     3121 agcatcaccc cggctttcac aagatcttcg ttgtacttca ccattccagc gattagcttc
     3181 tcatctggaa gaatgccttt ctcgctattc ttatcggctg gcatcaagac catgactctc
     3241 atcgtcgtac ctccttgtgg gcttgttttg gctcactttc ggccgcgcgt tcgagcgcgg
     3301 cgatgtcgag cttcttcatc ttgagcatgg cttccatcac gcgccctgcc ttagtgcggt
     3361 ccggatcggc aaggagccgt tgcagcatcg tcggcacaat ctgccacgag aggccgaagc
     3421 ggtctttcac ccaaccgcac tgttccgact cgggaaccgc cgagagggcc ttccagtaac
     3481 ggtccacctc tgattggtct tcgcaaggga tggtgaatga gactgcttcg ttgaacttga
     3541 agtacggccc gccattgagg ccgctgaaac tctggcccat caaggtgaac tcgacgacca
     3601 ggac
//
LOCUS       DBNGSB010000076         8206 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3577536, whole genome shotgun sequence.
ACCESSION   DBNGSB010000076 DBNGSB010000000
VERSION     DBNGSB010000076.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 8206)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8206)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8206
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3577536"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="AC159B_02135"
     CDS             complement(<1..467)
                     /locus_tag="AC159B_02135"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HIR3376585.1"
                     /translation="MSMALPIIDLKGTPYEGGVQHGSAAEGLIRRNLEIYYDRFQRET
                     RLPPQEVRDRAMRYLTVIDAHDPEYGQAMRGVSEGAKISLADIAVLNARYEILYSQYA
                     AINQGEEATLMVDGCTAFAVTPDASVDGHLYLGQNWDWFPQVEGVIVRARRSDG"
     gene            complement(542..1537)
                     /locus_tag="AC159B_02140"
     CDS             complement(542..1537)
                     /locus_tag="AC159B_02140"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3376586.1"
                     /translation="MQQQLKTDITIRKFEERDYAGVVAVSNAVLPDRPGTVEELRYDD
                     EHYDKKFVNERYVADDRSSGTTLGYAGLWHSAWAFHPRKFGMEIRVHPDHQRNGIGSA
                     LWLRLQDALRDHQAIDVKTYIWEKIPSGIAFAARHGFYEVMRAWESRLEIGTFDFKRF
                     IPHVDQALASGMTITTLAAERARDAQNLKRIHALDVELSADVPRPPDETRTPIDFNMW
                     HDYAVEGPWSIDDAYFLAVVDGEYAGISNLFKPKTGDWLNQGLTGVRRRYRGRHIATA
                     LKVKTVEYARDHGIREIRTFNEINNAPMLAINGKFGFVRQPAWITFMKEFSGERP"
     gene            complement(1805..2452)
                     /locus_tag="AC159B_02145"
     CDS             complement(1805..2452)
                     /locus_tag="AC159B_02145"
                     /inference="COORDINATES: protein motif:HMM:NF017885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HIR3376587.1"
                     /translation="MLQLESGGRPFTTEEQTAIRAMAPVVPAVRHAQEARRSNAAVQA
                     REVLEALGAEIPVDVEAIAERLGYPVRWRAMPEGYRGGIEGDAGYRILVLNRDHPFRS
                     DAERRWVMAEELAHAVLGHTTLAASESAAREAYMKDDSRRTSETDARSFAAELLMPAA
                     EVRRSFASEQGTILRALGSEERVQAVKTVVADLAREFRVSQQAMRIRLAQLGLLA"
     gene            complement(2652..4904)
                     /locus_tag="AC159B_02150"
     CDS             complement(2652..4904)
                     /locus_tag="AC159B_02150"
                     /inference="COORDINATES: protein motif:HMM:NF019758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3376588.1"
                     /translation="MAQTRMANRQPARRPTSGKLRTILEDVGTPPAAAFVPAPFQEAA
                     LVAVRTSDVLVAAPTGSGKTWIAEQAMSELLAAGGTAWYTTPLKALSNQKYHRFSGIY
                     GVESVGLLTGERKINGQAPIVVATTEILRNALYGDAMTPNLIVLDEAHYLSDPERGTA
                     WEEILLLAPARSRLLLLSATFPNANSVAAWLGEVRGTKQQIIAEHTRPVPLRNIMADA
                     RGRLIPPDAVGHLPSGNRGSSWLPNLMRDLEHYSLLPVILFFPARRQCDEAARDLASL
                     RAFGPELRAAALARWEREFPYLKQHAFRTALIDSGVAPHHAGHTTAWRLAIEDLLARG
                     LVRAVCATTTLASGLDVPARTVALSTLVRNSPQGPMVLTATEFHQMSGRAGRRGKDTI
                     GVVVLPAASREEAREGLALIDAEPDPVTSSFTPGYVQVLNLLRRSTLQDALRELTRSL
                     AAFERRAEVMRLRDAIGSIPPDELVERPCDDRLFTRGRYERMLDRLRRLQKLGRSADE
                     EMTALKEEIGAWPCTTCPVEERCLATIEDLRAREHRRSSLRQALHKIEGALTDEFNRR
                     ASVLKRLGYLDESYRLTAEGMWAAELRHPRALVLAEIVRRSMVGGSTAAWAAVAGALA
                     TERAPYRGGEAGLNAMGRLVRELADLERQQALDPGEALKQFEPEWDPASRRRIPSPAD
                     RRADAVVAWMRGADWGQLLKESQSEEGDLQRIMLQAAEVLMQLEGLPFPDVRAAARDA
                     RLRLLRAPVV"
     gene            4858..6147
                     /gene="solA"
                     /locus_tag="AC159B_02155"
     CDS             4858..6147
                     /gene="solA"
                     /locus_tag="AC159B_02155"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003960510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HIR3376589.1"
                     /translation="MGRRAGWRFAILVCAIAVYDNAQVEAVSGTEQRPGSSQEPSRNE
                     TNPLMEPMAQFDVIVLGLGAMGSATAYHLARRGRRVLGLEQYTPAHDRGASHGHSRII
                     REAYFEDPRYVPLLQRAYELWDALQQQAGSRLLVMTGGLMIGPEDGELVQGTLTSAHQ
                     HHLAHELLTSADLKRRFPQFATDESTVAVWERRAGALFPEDCIRTHLSQAARAGAVLH
                     FEEPVQHWRVLGDGVEVTSSRGTYAAAHLVVTAGPWAGRLLQQRGLPLEVERNVMYWF
                     RPRDETPFMPDRFPIYIYEYTQGRFFYGFPALRGEGVKIAHHHSGDLCTPETIKREVT
                     EDEIQRIRALLARHIPDANGELLSTATCMYTNTPDYHFIIDRHPEHAAVTIACGFSGH
                     GFKFSSVVGEILAELAEYGHTRHDIDLFRLNRFASPV"
     gene            6178..6852
                     /locus_tag="AC159B_02160"
     CDS             6178..6852
                     /locus_tag="AC159B_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376590.1"
                     /translation="MSLRPGSPPTVPDRPTAAQLYWLGYISASGRVYAQRSVPALVVD
                     IDPRDLEHVHRIGEEFTDYRLHWELCRSNLNGLQGYIRDREFGKFLAQWGAPGAGEED
                     GWVSLALIPSSLLPHFVRGYLEGGHQTPPFGGSITPTILTSIRILSLMGPEGFLSQLS
                     DALGRHAGLPAGIMTRRPKGRHMLTYRGTHARRLIHYAYRDPSPSMPRAARLLHSFQK
                     RAKVTI"
     gene            6903..7301
                     /locus_tag="AC159B_02165"
     CDS             6903..7301
                     /locus_tag="AC159B_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA recognition motif domain-containing protein"
                     /protein_id="HIR3376591.1"
                     /translation="MGTKSLYVGNLSYDVTEQELRDMFQAWGPVAQTRLIADRGFGFV
                     DVPEEKAAEAIQAMNGKEHKGRSLMVNEARPRDDRGGGRGGDRGGYGKGGGGGYGGGG
                     GGRSGGGYGGGGGRGGKGGNRGGGGGRGRR"
     gene            7516..>8206
                     /locus_tag="AC159B_02170"
     CDS             7516..>8206
                     /locus_tag="AC159B_02170"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3376592.1"
                     /translation="MDFETLALHPETKRAIRQLGWTRPTPIQARVIPEMISGKDLVGQ
                     AQTGSGKTGAYGIPLVERLNATVRTLQGLVLVPTRELAIQVTDEIRALGRGSGFKVVT
                     LFGGQSIERQFSELRRHPQIAVATPGRLLDHVQRGTVKLGGVTIVILDEGDRMLDMGF
                     LPDVSRILSETPSTRQTALFSATMPTAIHGIAKQQMRDPVWIRVAAPMPTVNTVEQFY
                     LEVAEEDKVRAL"
BASE COUNT         1507 a         2622 c         2525 g         1552 t
ORIGIN      
        1 ccatcggacc gtcgcgccct cacgatgacg ccttccacct gcgggaacca gtcccagttc
       61 tgcccgaggt agaggtggcc gtccacagac gcatcggggg tgaccgcgaa cgccgtgcac
      121 ccatccacca tcaatgtcgc ttcctcgccc tggttgatcg ctgcatactg gctgtaaaga
      181 atctcatagc gcgcgttgag gacggcgatg tcggccagcg aaatcttcgc gccttcgctc
      241 acgccgcgca tggcctgtcc gtattcagga tcgtgcgcgt cgatcaccgt cagatagcgc
      301 atggcgcggt cgcggacttc ctgcggcgga agacgcgtct cgcgctggaa ccgatcgtag
      361 tagatctcca ggttccggcg gatgagacct tcggcagcgc tcccgtgctg cacgccgccc
      421 tcgtacggcg ttcccttgag atcgatgatc ggaagggcca tgctcattcc ctttcgctaa
      481 tcggacacat attcatttgg ctgaaatggt ctataacgtc ttctgaaatc ggcaagcgtc
      541 ttcatgggcg ttcccctgag aactctttca tgaacgtgat ccacgccggc tggcgcacga
      601 acccaaactt cccgttgatc gccagcatcg gcgcgttgtt gatctcgtta aaggtccgga
      661 tctcgcggat gccgtggtcg cgggcatact cgaccgtctt tactttcaac gcggtggcga
      721 tgtggcgtcc ccggtatcgc cggcgtacgc cggttaagcc ctgattgagc cagtccccgg
      781 tcttgggctt gaaaagattg cttatgcccg cgtactcgcc atcgacgacc gcaaggaagt
      841 acgcatcatc gatcgaccat ggcccctcca cggcgtaatc gtgccacatg ttgaagtcga
      901 ttggcgttct ggtctcgtca ggcggccgtg ggacatccgc agagagctcg acgtctaagg
      961 catgaatccg tttcaggttc tgggcgtctc ttgcccgctc agccgcgagc gtcgtgatcg
     1021 tcattccact tgcgagcgcc tggtcaacgt gaggaatgaa tcgcttgaag tcgaacgttc
     1081 cgatctccag ccgtgattcc cacgcccgca tcacctcgta gaatccatgc cttgcggcga
     1141 acgcgatccc tgacggtatc ttctcccaga tgtacgtttt cacgtcgatc gcctgatggt
     1201 cgcggagcgc atcttgcagc cgtagccaca gcgccgaccc gatgccgttc cgctggtgat
     1261 cggggtgcac tcggatctcc ataccgaact tcctgggatg aaacgcccac gccgagtgcc
     1321 acagtccggc atagccgagg gtggtcccgg acgaccgatc atcggcgacg taccgctcgt
     1381 tgacgaactt cttatcgtag tgctcatcgt catagcgcaa ctcttccact gtccctgggc
     1441 ggtccgggag gacggcgttg ctgaccgcga ccacaccggc gtagtcgcgt tcttcgaact
     1501 tgcggatcgt aatatccgtc ttcagctgct gctgcatgtt tcttactccc tcgacgtgaa
     1561 tttgcaaaca cgtgaatttg caaacaaaaa agcgcaggtc cgattcccct gcgcccacgt
     1621 cgtcgtgcga tcggcagtgc ctacccggca gtgccctcca tcatccggcg tccatgagaa
     1681 cagggccggt ccgtaagcgg accggatgcg gtatcgggaa tgaagttgag cctgatcacc
     1741 attgggacac cctctgacga acgggctgac agtagcacag cctgcgggac gaagtcaata
     1801 cgaactacgc gagcagcccg agctgggcga gtcgaatcct catggcctgt tgggaaacgc
     1861 gaaactcacg ggcgaggtct gccaccacag tttttacggc ctgtacccgt tcctcactcc
     1921 ccagggcccg aagtatggtg ccctgctcac tggcaaagga gcgtcgcact tccgcagcgg
     1981 gcatcaggag ttcggcggca aatgacctgg catcggtctc gctcgtccgg cgggaatcat
     2041 ccttcatgta cgcttcccgc gcggcacttt cgcttgccgc gagggtcgta tgtcccagca
     2101 cggcgtgggc gagctcttcg gccattaccc aacgacgctc agcgtcggac cggaatggat
     2161 gatcgcgatt caacacgagg atccggtagc cggcatcgcc ctcgatcccg ccacgatacc
     2221 cttcaggcat cgcgcgccag cgaacgggat agcccagccg ctcggcgatc gcttccacgt
     2281 cgacgggtat ctccgctcct aaggcttcga gaacctcacg tgcctgtacc gccgcgttgg
     2341 agcggcgcgc ctcttgcgcg tggcggacgg cgggaacgac tggggccatc gcgcggatcg
     2401 ccgtctgctc ctctgtcgtg aacgggcgtc cgccgctctc cagctgcagc aatccgcaat
     2461 tggaacaggc ccacgcaacc ggcggctcgc actcgctggc gaagcgagga gacatctgag
     2521 actcacacac tgggcaagaa cgcatgagtg ctccaactac gggataacgg ggataaaggg
     2581 ataaggggat aaggtaacaa cagttttcgt tatcccttta tccccatatc cctttatccc
     2641 gccgtttcgt ttcagacgac cggcgctctc agcaacctca gtctcgcgtc gcgcgccgcg
     2701 gcgcggacgt ccgggaacgg cagcccctcc aactgcatca gcacctcggc cgcctgcaac
     2761 atgattcgct ggagatcacc ttcctcggac tggctttcct tcagaagttg cccccagtcg
     2821 gcgccgcgca tccaggccac aacggcgtcc gcacgccggt cggcgggtga tgggatacgg
     2881 cgacgggacg caggatccca ctccggctcg aactgcttga gcgcctcgcc gggatcaagc
     2941 gcctgctggc gctcgagatc cgcgagttct cgaacaagtc ttcccatggc attgagtccg
     3001 gcctctcccc cccgatacgg cgcgcgctct gtggcgagcg cccccgcgac ggccgcccag
     3061 gccgccgtcg agccgccgac catgcttctg cgcacaatct cggcgaggac cagcgctcga
     3121 ggatggcgca gctccgccgc ccacattccc tcagccgtga gtcgatacga ctcatcgaga
     3181 taccccagtc gtttgagcac cgaggcgcgc cggttgaact cgtcagtgag ggctccttca
     3241 atcttgtgga gcgcctgcct gagagaggaa cgacggtgct ctcgcgcccg cagatcctcg
     3301 atggtcgcca gacaccgttc ttcgacgggg cacgtcgtgc agggccacgc cccaatctct
     3361 tccttcagtg ctgtcatttc ctcgtctgcg gaacgcccca acttttgcag ccgtcgcagc
     3421 cggtcgagca tgcgctcgta ccttccgcgc gtgaacaggc ggtcatcgca gggccgttcg
     3481 acgagttcat ccggtggaat cgagccgatg gcgtcgcgca gccgcatcac ctcggcgcgg
     3541 cgctcgaacg ccgcaagcga tcgggtgagt tcgcgcaggg cgtcctggag cgtgctgcgc
     3601 ctgaggaggt tgaggacctg cacgtagccc ggagtgaacg atgaggtgac gggatccggt
     3661 tcggcgtcga tcagcgccag cccctcgcgg gcttcttcgc gcgaggccgc gggcagcacc
     3721 acgacgccga tcgtgtcttt tccgcgccgg ccggcgcgtc cgctcatctg gtggaactca
     3781 gttgccgtca gcaccatcgg accttgggga ctgttccgca cgagcgtcga aagcgcgacc
     3841 gtgcgcgccg gtacgtccag cccgctggcc agcgtcgtgg tcgcacacac ggcacgcacc
     3901 aggccgcgcg ccagcagatc ctctatcgcc aggcgccacg cggtggtgtg cccggcgtga
     3961 tgtggcgcaa cccccgagtc gatcaacgcg gtgcgaaacg catgctgttt caggtacgga
     4021 aactcccgct cccaacgcgc gagcgccgcg gcccggagct ccggaccgaa tgcgcgcagc
     4081 gacgcgaggt cgcgggcggc ttcatcgcat tgtcttcgcg ccgggaagaa caagatcacc
     4141 ggcaggaggc tgtagtgctc gagatcgcgc atgagattgg ggagccagga cgaccctcga
     4201 ttgcccgacg gcaggtggcc gacggcatcg ggcggaatca agcggccgcg cgcatcggcc
     4261 atgatgttgc gcagcggaac cggacgcgtg tgctcggcaa tgatctgctg tttggtgcct
     4321 cgcacctccc ccagccatgc cgcgaccgaa ttggcattcg ggaacgtggc ggacagcaac
     4381 agaaggcgag agcgtgccgg agcaagcagc aagatctctt cccacgccgt tccccgctcc
     4441 gggtctgata gatagtgcgc ctcatcgagc acgatgaggt tcggcgtcat cgcgtcccca
     4501 tacagggcgt tgcggagaat ctctgtggtg gcaacgacga tcggcgcctg accgttgatc
     4561 ttccgttcac ccgtcaagag tccgaccgat tcgacgccgt agatcccaga aaagcgatga
     4621 tacttctgat tcgagagggc cttgagcggc gtagtgtacc aggcagtgcc cccggccgca
     4681 agcagttcag acatggcctg ctcggcgatc caggtctttc cgctccccgt gggcgcggcc
     4741 accagcacgt cactcgtccg cacggccacg agcgcggctt cttgaaatgg cgcagggaca
     4801 aacgccgctg caggcggtgt gccgacatcc tcgagaatcg tgcgaagctt gccagacgtg
     4861 ggccgacgtg cgggctggcg gttcgccatc cttgtctgtg ccattgccgt ctatgataac
     4921 gcacaagtgg aagccgtgtc aggcacggag cagcgtccag ggagctccca ggaaccgtcg
     4981 cgaaacgaaa cgaatccgct gatggagccg atggcacagt tcgacgtgat cgttttgggg
     5041 ctcggcgcga tgggcagcgc caccgcctat cacctcgcgc gccggggacg ccgggtgctg
     5101 gggctcgagc agtatacgcc ggcgcatgac cgcggcgcct ctcacggtca ctcccgcatt
     5161 atccgggaag catactttga agacccgcgc tacgtgccgt tactccagcg cgcctatgag
     5221 ctgtgggatg cgctccagca gcaagccgga tcgcgtttgt tggtcatgac cggcggcctg
     5281 atgatcggcc cggaagacgg tgaactggtc cagggcacgc tcaccagcgc gcaccagcac
     5341 catctggctc acgaattgct gaccagtgcc gacctcaaac gccgctttcc gcaatttgcc
     5401 acggacgagt ctactgtggc cgtatgggaa cggcgcgccg gcgctctgtt tcctgaggat
     5461 tgcatccgca cccatctttc gcaagcagcg cgggccggtg cggtcctgca ctttgaagaa
     5521 ccggtacagc actggcgcgt tcttggcgac ggcgtagagg taacgtcgtc ccgcggtacg
     5581 tatgcggcag cccatttggt cgttaccgcc ggtccctggg ccggccggct gttacaacag
     5641 cggggactcc ctctggaggt cgaacgcaac gtcatgtact ggttccggcc ccgtgacgaa
     5701 acgccgttca tgccggaccg ttttccaatc tacatctacg agtacacgca ggggcgattc
     5761 ttctacgggt ttcctgcgct tcgtggagaa ggcgtcaaga tcgcgcacca tcacagcggc
     5821 gatctgtgca cgcctgagac catcaaacgc gaggtgactg aggacgagat ccagcggatt
     5881 cgtgccctgc tcgcgcgcca cattcccgat gccaacggcg agctgctgag cacagccacc
     5941 tgtatgtata ccaacacgcc cgactatcac ttcatcatcg accgccatcc cgagcacgcc
     6001 gctgtgacga ttgcctgcgg cttctccggc cacggtttca agttctcatc ggtagtcgga
     6061 gaaatacttg cggaactggc cgaatatggt cacacacgac atgacatcga tctgttcagg
     6121 ctgaaccgtt tcgcatcgcc agtgtgagtt ctccgaagac tatggtaaaa tgaatagatg
     6181 agcctccgtc ctggatctcc tcccaccgtt ccggatcggc cgaccgcggc gcagctatac
     6241 tggctgggtt acatttccgc gtcgggtcgt gtttacgcgc agcgatcagt tcctgcactc
     6301 gtggtcgaca tcgatccgcg agatctcgag cacgtgcaca ggattggcga agagttcacc
     6361 gactaccggc ttcactggga actgtgccgc agcaacctga acgggttgca aggttacatc
     6421 cgagaccggg agtttggcaa gttcctcgcc cagtggggcg cgcccggggc gggtgaggag
     6481 gacgggtggg tctcactcgc gttgatccca agttcgctgt tgccgcactt cgtccgaggc
     6541 tacctcgaag gcgggcacca aacgcccccg ttcgggggca gcatcacgcc gacgatcctc
     6601 acatccattc gaatcttgtc gctgatggga cctgagggat tcctctccca gctcagcgac
     6661 gcattgggcc ggcatgccgg cctccccgcc ggcatcatga cccggcgccc caaaggacgg
     6721 cacatgctga cgtatcgtgg tactcatgcc agacgcctca tccattacgc ctatcgcgat
     6781 ccttcgccgt caatgccgcg ggcagcgagg ctgttgcact cgttccagaa gcgggctaag
     6841 gttacaatct gaggatctca aatggaggcc attgaagcag atcgcttaaa gggagtgaac
     6901 tgatgggaac caagagtctc tacgttggca acttgtcata cgacgtcacg gagcaggagc
     6961 tccgcgatat gtttcaagcc tgggggcctg tggcgcagac ccgtctcatt gcggatcgag
     7021 ggttcgggtt tgtggatgtg ccggaagaga aggcggcgga agccattcag gcgatgaacg
     7081 gcaaggagca taagggccgc tcgctgatgg tcaacgaggc gaggcctcga gacgatcgag
     7141 gcggagggcg cggtggcgac cggggcggtt acggcaaggg cggcggcggt ggctacggtg
     7201 gtggcggtgg tggccgcagc ggcggtggct acggtggcgg cggcggtcga ggcggcaaag
     7261 gtgggaaccg tggaggcgga ggcgggcgcg gcagacgctg accgctttgc aaccccgggc
     7321 gtttgactga aacggccggt cctgctacac taacgataac cgtcataaag cgttctgcga
     7381 gggatgcgaa gtcggcatcc ctcgcgttgt gtatcgcgag ccttcttcgt ggtgggttgg
     7441 ttgcggcgag acacgcgcag atgccggcaa ctaactcacg gtccggtaga accggatccg
     7501 aaggaggcta tttctatgga ctttgaaaca ctcgcgctcc atccggagac gaagcgggca
     7561 atccgccagc tcgggtggac gcgacccaca ccaatccagg cccgcgtcat cccagagatg
     7621 atctccggca aagacctggt cggccaagcc cagacggggt ccggcaagac cggtgcgtac
     7681 ggtattcccc ttgtggaacg cctcaacgca accgtccgga cactgcaggg tctcgtgctc
     7741 gtgccaacgc gcgagctggc cattcaggtc accgacgaga ttcgcgcact gggccgcggc
     7801 tctggcttca aggtggtcac gctgttcggc gggcaatcta ttgagcgtca gttctcagaa
     7861 ctgcgccgcc atccgcagat cgccgttgcc actcccggcc ggttgctgga tcacgtccag
     7921 cgcggcacgg tcaaactcgg cggagttacc atcgtcatcc tggatgaagg cgatcgcatg
     7981 ctggacatgg gctttctgcc agatgtctct cgcatcctga gcgagactcc gtcgacgcgg
     8041 cagaccgcgc tcttctccgc cacgatgccg actgcgatcc acgggatcgc gaaacagcag
     8101 atgcgcgacc cggtttggat tcgtgtggcg gcaccgatgc ctacagttaa cacggttgag
     8161 cagttctatc tggaggtcgc agaagaagac aaagttagag ccctcc
//
LOCUS       DBNGSB010000077         5529 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3577704, whole genome shotgun sequence.
ACCESSION   DBNGSB010000077 DBNGSB010000000
VERSION     DBNGSB010000077.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 5529)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5529)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5529
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3577704"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1705
                     /locus_tag="AC159B_02175"
     CDS             <1..1705
                     /locus_tag="AC159B_02175"
                     /inference="COORDINATES: protein motif:HMM:NF017633.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990112 - RQC complex [Evidence IEA];
                     GO_function: GO:0001968 - fibronectin binding [Evidence
                     IEA];
                     GO_process: GO:0072344 - rescue of stalled cytosolic
                     ribosome [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein"
                     /protein_id="HIR3376593.1"
                     /translation="DSVVLAAVAVDLEEYVGGRIVRIAQPSETEIILDIRRGRATGRL
                     LISIHPQWARVHLTKTGQRGALSSFAQMLRRRLERGLLRSTHQPPFERTLWLDIDADG
                     SRAGLVIEIMGRHSNLILVENGHIAGSLKTVPRSKSSVREVRPGTPFVPAPRDRSTPL
                     ELTPELLAANLANARVPLARALTSSVLGLSPALAAELVFRSGLRPDETADEHADAASR
                     LWPHLQELVAIVEEQRFAPMVYADEGGVSGFTPFPFRHLDALRHQPADSMSAAVDTVL
                     TTAGSLSVLEDARARTVAAVDAALEKIRRTHDEVAKSLVEAEAAKDLRAQGELLLAYA
                     SQVEPGAVEARIAGYDGKPLVIRLDPELSPVENAQRLFKRYGKLRDARASLQTRRQRL
                     TSDRNFLVDARAMALKATTREHLDDINRELVDEGFLRAQKLSRGREKSAGPRAFKLGG
                     GTVLVGRTKSENDQVTFTLASPQDMWFHARGVGGSHVVLKTERKQPSPTLISQAASIA
                     AYFSQARESGQVPVDYTPRKYVRKPKGAKPGLVTYEKEKTIFVKPALPETGKRGNGET
                     R"
     gene            complement(1713..2303)
                     /gene="pyrR"
                     /locus_tag="AC159B_02180"
     CDS             complement(1713..2303)
                     /gene="pyrR"
                     /locus_tag="AC159B_02180"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HIR3376594.1"
                     /translation="MKLRPTMREKAKVMDADAIRRALVRMAHEVLERNGRADILALVG
                     VPTRGVQLAQRLASIIDRIESSSLPVGTLSIANYRDDIGRAPATHTASASLPFDVAQR
                     AIILVDDVLYTGRTVRAALDALIDLGRPQSIQLAVLIDRGHRELPVRPDYVGKNLPTS
                     SKEHVAVRLQEIDGVDEVVIEEPTDGNEESRNRGNA"
     gene            complement(2305..3252)
                     /locus_tag="AC159B_02185"
     CDS             complement(2305..3252)
                     /locus_tag="AC159B_02185"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019558980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR3376595.1"
                     /translation="MNEPPKAHHTYFVDEAHGGTRLDAFLARHLPTVSRSRIKTLIAS
                     GRVSVDGADAKPATPVRRGQRVEVDVPALPRVELQPEPIPLDVLYEDDQLMVINKPAG
                     LTVHPGAGRPQGTLANAILARIPQLRGDTDRPGIVHRLDKDTSGALVVAKTPEAQASL
                     QAQVAARSAGRRYMALVHGDVRQDEGTIAARIGRHPRRRTKMAVRPDGREAVTHYRIV
                     ERFPGFTMIEARLITGRTHQIRVHFAHLGHPVVGDPRYGGRRETLGLGRQALHAWKLE
                     FTHPQTGRVMSFEAPLPEDIAQVLAWLRAREKEEGKVES"
     gene            complement(3249..4025)
                     /locus_tag="AC159B_02190"
     CDS             complement(3249..4025)
                     /locus_tag="AC159B_02190"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR3376596.1"
                     /translation="MRPILVEFGDCATRCIPISSFGLFLLLAFVAGITVARRRGRALG
                     IDPSQMLDLSLYMIIGGIIVGRLGYAAINWPTFAGSPLSIFTIWKDSGLTFYGALLGG
                     LIVAGVFAHTRKIPLARLMDVFAPALALGYAVAMVGALLHGLYVGRASGVPWAVTMLL
                     DRRHPTQIYLLLASLGTYQVLKAQERQAVMPGVLFYLWLLLFSLARVAVEFFVESPAI
                     FGPFTLAQIVNVAAAVIAVIGLVVSARRPAAEPEVASPTV"
     gene            complement(4029..4484)
                     /gene="lspA"
                     /locus_tag="AC159B_02195"
     CDS             complement(4029..4484)
                     /gene="lspA"
                     /locus_tag="AC159B_02195"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HIR3376597.1"
                     /translation="MRLPSLATVVAVVALDQAIKVVVERTIPVGASLPLVPPVLFLTN
                     VRNPGIAFSALPGIPLIVPAAIALTLIFLLFYNKTRWSDRPTVSFGVALLAGGALGNL
                     IDRVRVGAVIDYLDLRVWPVFNLADVAITAGAALLILTYAVRPGTQRAR"
     gene            complement(4496..5002)
                     /locus_tag="AC159B_02200"
     CDS             complement(4496..5002)
                     /locus_tag="AC159B_02200"
                     /inference="COORDINATES: protein motif:HMM:NF013427.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HIR3376598.1"
                     /translation="MAKAMKPARARVARKPVKSRAAAPKAAQRSSVRPMKGNKVRSLS
                     KRELEEFRKMLEEERERLTEELEAMEEHAPEVEEQVGMDIGGGYDEDLADVASSTFER
                     EKGLALESSVQAMLAQVEEALSRIDDGSYGVCQRCGDAIDAARLRVLPYATLCIRCKE
                     LEEKSPVR"
     gene            complement(5145..>5529)
                     /locus_tag="AC159B_02205"
     CDS             complement(5145..>5529)
                     /locus_tag="AC159B_02205"
                     /EC_number="5.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013353.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047661 - amino-acid racemase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HIR3376599.1"
                     /translation="GDAVPGLRQVGVLATAATVQSRLYHDAFERVGVAVVGPDERGQD
                     VVNRAIYGVKAGRMGPEVTRELSAVATALISSGAQAIVLGCTELPFVLREQDVSVPLL
                     DSNLILASAAVRAATTAPEPVSPRR"
BASE COUNT         1039 a         1777 c         1713 g         1000 t
ORIGIN      
        1 tgacagcgtc gtgctcgcgg cggtggctgt tgatcttgag gaatatgtcg gcgggcggat
       61 cgtacgcatc gctcagcctt cagagactga aatcatcctc gacatcaggc ggggccgagc
      121 gacgggacga cttctgatat caatccatcc tcaatgggcg cgcgtccacc tcacgaagac
      181 cggtcagcgg ggagccctgt cgtcgtttgc ccaaatgctg cgccgccggt tggaacgcgg
      241 gctgctgagg tccacgcacc agccgccgtt cgaacgcacg ctctggttgg acatcgatgc
      301 cgacggttca cgcgccggtc tcgtcatcga aatcatgggg cggcatagca acctgatcct
      361 ggttgagaat gggcacatcg cggggagtct aaaaacggtg ccgcgctcca aatcctccgt
      421 acgggaagtg agaccgggga cgccgtttgt gccggctcca cgtgatcgat ccaccccgct
      481 cgagctcaca cctgaactgc tggccgccaa tcttgcgaac gcgcgcgtac cgctggccag
      541 ggcgctcacc tccagcgtcc ttggcctgag tcctgcgctc gccgctgaac tcgtgttccg
      601 cagcggccta cgacccgacg aaacagcgga cgaacacgcc gatgccgcgt cgcgcctatg
      661 gcctcacctg caagagcttg tcgcgatcgt tgaagaacag cggtttgcgc cgatggtcta
      721 cgcggacgag ggcggtgtga gcggcttcac gccgtttccg ttcagacatc tcgatgcact
      781 gcggcatcaa ccggcagaca gcatgagtgc cgccgttgac acggtcctga cgaccgcggg
      841 atctctgtcc gtcctcgagg atgcgcgcgc ccggacggtc gcagccgttg acgcggcgct
      901 ggagaagatc cggcgcacgc acgacgaggt ggcgaagtcg ctggtagagg cggaagcggc
      961 gaaagacctt cgcgcgcagg gagaattgtt gctcgcgtac gcttcgcagg tcgagcccgg
     1021 tgcggttgaa gcgcgtatcg ccggctacga tggcaagccc ttggtgatac gtcttgatcc
     1081 ggagctttcc cctgttgaga atgctcagcg tctcttcaag cgatacggaa agctgcgtga
     1141 cgctcgggcg tcgctgcaga cgaggcggca gcggctaacc tcggaccgaa acttcctggt
     1201 agacgcccgc gccatggccc tcaaggcgac gacgagggag catctcgacg acatcaatcg
     1261 tgagcttgtg gacgaaggct ttctacgagc tcaaaaactg tctcggggac gcgagaaatc
     1321 ggccggcccg cgcgccttca aactcggagg cggtaccgtg ctggtcggcc ggacgaagag
     1381 tgagaacgat caggtgacgt tcaccctggc gtcaccccag gacatgtggt tccacgcgcg
     1441 cggggttgga gggtcccacg tggtgctcaa gacggaacgt aaacaaccca gtccaacgct
     1501 gatctcacag gctgcctcaa ttgctgcata tttcagccag gctcgggaat cgggacaggt
     1561 ccctgttgac tacacaccga ggaagtacgt ccggaagccg aaaggggcga agccggggct
     1621 cgttacgtac gaaaaagaaa aaacgatatt cgtgaaaccc gcattaccgg aaacagggaa
     1681 acggggaaac ggggaaacgc gataagcacg tactacgcgt ttccgcgatt ccgcgattcc
     1741 tcgtttccgt cagtcggctc ttctatcacc acctcatcca ccccatcgat ctcttgcagt
     1801 ctcacggcga cgtgttcttt ggacgacgtg ggcaggttct tccccacgta gtcaggtcga
     1861 actggcaact cccgatggcc ccggtcgatg agcaccgcga gttggatgct ttgcgggcgg
     1921 cccaggtcaa tgagcgcgtc tagtgcggcg cggacggtgc gccccgtgta cagcacgtcg
     1981 tcgaccagaa tgattgcgcg ctgcgccaca tcgaacggca ggctggcgct cgcggtgtgc
     2041 gtcgcaggag cgcggccgat gtcgtcccga tagttcgcaa tgctgagagt tcccaccggc
     2101 agcgacgagc tctcgatacg gtcgatgatc gatgcgagcc gctgcgccag ctgtacgcct
     2161 cgtgtcggaa cacccaccag cgcgaggatg tcggcccgac cgttgcgctc gagcacttcg
     2221 tgggccatgc ggacgagcgc gcggcggatg gcatcggcat ccatcacttt ggccttctcg
     2281 cgcatcgtcg gtcgcagctt catctcaact ctccactttt ccctcttcct tctcccttgc
     2341 gcggagccat gctaggacct gcgcgatgtc ctccgggagc ggtgcctcga acgacatgac
     2401 ccggccggtc tgcggatgcg taaactccag cttccacgca tgcagcgcct gccgccccag
     2461 accaagcgtc tctcgccggc ctccgtaccg cggatctccg acgaccggat gtccgaggtg
     2521 cgcgaagtgg acgcggatct gatgcgtgcg tccggtgatg agcctggcct caatcatggt
     2581 gaaacccggg aatcgctcca cgatccggta gtgcgtcacc gcctcacgtc cgtcggggcg
     2641 caccgccatc tttgtgcggc ggcgaggatg tctgccgatg cgcgcagcga tcgtcccttc
     2701 gtcctgccgg acgtcgccgt gaaccagcgc catgtatcga cgacctgcgg atcgagcggc
     2761 cacctgcgcc tgcagcgagg cttgcgcctc cggggtcttt gccaccacga gtgcgccgga
     2821 cgtatccttg tccagccggt gcacgatccc ggggcggtct gtatcgccac gaagttgagg
     2881 aatacgcgcc aggatcgcat tcgccagggt tccctgtggg cggccggctc ccggatgcac
     2941 ggtgagaccg gccggcttgt tgattaccat gagctggtcg tcttcgtaca agacgtccag
     3001 cgggatcggt tcgggctgca actcaaccct gggcagcgcg gggacgtcga cttcgacgcg
     3061 ctgtccgcgc cggacgggag tcgccggttt cgcgtccgcg ccgtccacgc tgacgcggcc
     3121 ggaagcgatc agcgtcttaa tgcgagaccg tgacacagtg ggaaggtgcc gggcgagaaa
     3181 cgcgtcgagg cgcgtgccgc cgtgcgcctc gtcgacaaaa tacgtgtgat gcgctttggg
     3241 tggctcgttc acacggtcgg cgacgcgacc tcaggttctg cggcgggtcg ccgtgccgac
     3301 acgactaagc cgatgaccgc aatcaccgca gctgcgacgt tcacgatctg cgccagcgtg
     3361 aacggcccga aaatagcagg gctctcgacg aaaaattcca ccgcgacacg ggcgagggag
     3421 aacaacagca gccacaggta gaacaacact cctggcatga ccgcctgccg ctcctgtgcc
     3481 ttgagcactt gataggttcc cagtgacgcg agcaacaggt agatttgtgt gggatgccgg
     3541 cggtccagga gcatcgtgac cgcccacggc acgccgctcg cccggcccac atacagaccg
     3601 tgaagcagtg ctccgaccat cgccaccgca tagccgagcg ccagggcggg cgcaaacacg
     3661 tccatgagcc tcgccagtgg aatcttccgc gtgtgcgcga agacccccgc gacgatcagg
     3721 ccgccaagca gtgctccgta gaacgtcagg ccggaatcct tccagatcgt gaaaatcgaa
     3781 agcggcgaac cggcgaacgt gggccagtta atggcagcgt atccgaggcg gccgacgatg
     3841 atcccgccga tgatcatgta caggctcaaa tccagcatct gggacgggtc gatacccagc
     3901 gctcgcccgc gtcgccgcgc gactgtaatg ccggccacaa atgccaggag caggaagagt
     3961 ccaaatgagg aaatcgggat gcaccgtgtg gcacaatccc caaactcaac caggatgggt
     4021 ctcatccgtc acctcgcgcg ttgcgtgccg ggccgtaccg cataggtcag gatcaagagc
     4081 gcagccccgg ccgtgatagc cacatcggcc agattgaaca ccggccagac ccgaaggtcc
     4141 aggtaatcga tcaccgcgcc gacgcgtacc cggtctatca agttcccgag ggcgcctccc
     4201 gcaagcagcg cgacgccaaa gctcacggtc ggacggtccg accaccgcgt tttattgtag
     4261 aagaggagaa aaatcaaggt caaggcaatg gcggccggga cgatcaatgg aattcccggc
     4321 agcgcgctga atgcgatccc gggattgcgg acgttggtca agaacagcac ggggggaacc
     4381 agcgggaggg acgcgcccac gggaatggtg cgctcgacga ccacctttat cgcctgatcc
     4441 aacgcgacca ccgcgacgac tgtcgccagg gaaggcagcc gcatcgggcg ccgtgttagc
     4501 gtacaggact cttctcttcg agttccttac agcggatgca gagggtcgcg taggggagca
     4561 cccggagacg tgcggcgtcg atggcatctc cgcaccgctg gcagaccccg taggagccgt
     4621 catcaatccg cgagagcgct tcttcgacct gggccagcat ggcctgcacg cttgattcca
     4681 gcgccagccc cttctcccgt tcgaaggtgc tgctggccac gtctgcaaga tcttcgtcgt
     4741 agccgccgcc gatgtccatc ccgacctgct cctcgacttc aggagcgtgt tcttccatgg
     4801 cctcgagctc ttccgtaagc cgctctcgtt cttcttcgag catcttgcgg aattcttcga
     4861 gctcccgttt tgacagcgat ctgaccttgt tccccttcat gggccggacc gagcttctct
     4921 gcgccgcctt gggagcagcg gcgcgactct tgactggttt ccgcgccact cttgccctgg
     4981 ccggtttcat tgcttttgcc attcggaagc ctcctgcata cttacttcct cttaattccc
     5041 ctgcgaaaac gctgttttcc cgggatttac tccccgtagt gtatccattg ccctttgcat
     5101 tgacaagagg tgacgcatcg gctcaaatgt cgaggtgttc gaggctaacg ccttggggaa
     5161 actggttcgg gcgcggttgt cgcggccctc acggccgcgc tcgccaggat caaattggaa
     5221 tcgagcagtg ggacactgac gtcctgctca cgcagcacga atggcaattc cgtacaacca
     5281 agcacgatgg cctgggcgcc gctcgagatc agcgcggtcg ccacggcgga gagttcccgg
     5341 gtcacttccg ggcccatccg tccggctttg acgccataga tagcccggtt caccacgtcc
     5401 tgaccccgtt cgtccgggcc gaccacagcc acccctacgc gctcaaacgc gtcgtggtag
     5461 agacgagatt ggacggttgc agccgtcgcc aggacaccga cttgtcgcag cccagggacg
     5521 gcatcgcct
//
LOCUS       DBNGSB010000078         2204 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_205320, whole genome shotgun sequence.
ACCESSION   DBNGSB010000078 DBNGSB010000000
VERSION     DBNGSB010000078.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_205320"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="AC159B_02210"
     CDS             <1..557
                     /locus_tag="AC159B_02210"
                     /inference="COORDINATES: protein motif:HMM:NF014582.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIR3376600.1"
                     /translation="GAILTHGPPAALIILVGYLLVHLTQHTLAPHFHFGEEVHHVTKV
                     VSFSALAGLMLHTFVDGVAIASGFGVSDGLGILVFLAILLHKLPEGLAISSLFLAAGE
                     SRGAALGASAALAVSSLLGALLTQQLPLLEEYGLPLAAGVTLYVGASNLVPEFQGKKG
                     WNLPVSFFLGCALYYLARRLVGAG"
     gene            575..1663
                     /locus_tag="AC159B_02215"
     CDS             575..1663
                     /locus_tag="AC159B_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR3376601.1"
                     /translation="MSAMIRAINGAVMLAIVGSLACDEATAPQPLLALPMTDRIVFES
                     DRDDPLGDIYAMTVDGADVRRLTSSGVGETCPSISPDGNWIAYYVHVEGDTNFALWLM
                     RANGTEPRPLAVLGVPFQCPIWSRESDAIQTTVVLPDPPDNGTPTRSQVFNLAGLELA
                     RFQTVAATLAEFSADGTELLGQWSNCGANGCTDPEIGVRKLDGSGHRFLTGMVANGSF
                     IYEGARDPSLGPDGATVVYICDNSLGQSLNAVCTIQWDGTGKRRIVDEGWASPKLSPN
                     GERVSFLCPQGGSRDLCVIDIDGTNRMQWPANASEGAAWTPDGSSLAFVCGQKDICLI
                     RSVVGTLTNLTQGHGTNANPSVAATVSQ"
     gene            1821..>2204
                     /locus_tag="AC159B_02220"
     CDS             1821..>2204
                     /locus_tag="AC159B_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019184752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3376602.1"
                     /translation="MRPRTLDEFVGQEHLLAPGKALRDAIEKGSVSSMVFWGPPGSGK
                     TTLGRLIAQYTDRDFIPFSAVTEGIPRVREVVTDAEQRLQMGRQTILFVDEIHRFNKA
                     QQDAFLPHVEAGTITLVGATTENPSF"
BASE COUNT          427 a          659 c          696 g          422 t
ORIGIN      
        1 cgggcgcgat tctgacacac ggtccgccgg ccgcgctcat tatcctggtc ggctacctgc
       61 tcgtgcatct cacccagcac accctcgcgc cgcatttcca tttcggtgaa gaagtccacc
      121 acgtcactaa ggtggtgagc ttctcggcgt tggcggggct gatgctccac accttcgtgg
      181 atggcgtcgc aatcgccagc ggattcggcg tgagcgacgg cctgggtatc ctcgttttcc
      241 tggcgatctt gctgcacaag ctgcccgaag gtctggcgat ctctagcctc ttcctcgccg
      301 ccggcgagag caggggagcg gcactaggag cgagtgcggc gctagcggtg tcgagcctcc
      361 tcggcgcgct actgacgcag cagcttcctc tcctcgagga gtacggacta ccactcgccg
      421 ccggagtaac gctttatgtc ggcgcgtcga atctcgttcc cgaatttcag ggcaagaaag
      481 ggtggaacct gcccgtcagc ttcttccttg gctgcgcgct ctattatctt gcccgccgcc
      541 tcgtgggcgc agggtgaaaa actgaggagg agaaatgtca gctatgattc gggcgatcaa
      601 cggtgcggtc atgctagcta tcgtcggcag tctggcctgc gacgaggcga cggctcctca
      661 gcccctgctg gcattgccga tgaccgaccg gatcgtgttt gaaagcgacc gcgacgatcc
      721 gctcggcgac atttacgcga tgacggttga cggggctgac gtcaggcggt tgaccagctc
      781 tggtgttggt gagacgtgtc ccagcatctc acccgacgga aactggattg catactacgt
      841 tcatgtggaa ggcgacacaa actttgctct ctggctgatg cgcgccaatg gcaccgaacc
      901 ccggccgctc gcggtcttag gggttccgtt ccagtgcccg atttggtcgc gggagagcga
      961 tgcgatacaa acgacggtcg tccttcctga ccctcccgat aacggcacgc caacacggag
     1021 tcaggtattc aatctcgccg gacttgagct cgcccgtttt cagacagttg ccgccaccct
     1081 agcggagttc agcgcggacg ggacagaatt gctgggacag tggtcaaact gcggcgccaa
     1141 cggctgcacc gaccccgaga tcggtgtcag gaaacttgat ggtagcgggc accgctttct
     1201 taccgggatg gtcgccaacg ggagcttcat ctatgagggc gcgcgcgatc caagtctggg
     1261 gccggacggg gcgactgtgg tttacatctg cgacaactcg ctcgggcaga gtctcaacgc
     1321 ggtatgcacg atccaatggg atggaaccgg caaaaggcga atagttgatg agggttgggc
     1381 cagcccaaag ctctctccga acggagagag agtttccttc ctctgccctc aaggcggttc
     1441 gcgtgacttg tgcgtgattg acatcgacgg gaccaacagg atgcaatggc cggccaacgc
     1501 cagcgaaggc gcggcctgga caccagacgg gtcgagcctg gccttcgtgt gcggacagaa
     1561 ggacatttgc ttgatccgca gtgtcgtcgg tacgctgacc aatctcaccc agggtcacgg
     1621 aacgaacgcc aatccttccg tggcggcgac ggtttcgcag tagcgaaggg taacgacttc
     1681 aaagcggtca ggccggcgcc ttgcaccagg agttgctacc aggaatgcca aagaaattcc
     1741 gccgcccgcc ctctaacgtc tcctcgcgcg aagaaagcct gtggagcgcc cgcgaaaccg
     1801 cgcagccact ggcagcgagg atgcgtccga gaacgctcga cgagttcgtc ggacaggagc
     1861 atctgctcgc tccgggcaag gcgctgagag acgccataga aaagggaagc gtcagctcga
     1921 tggtgttctg gggaccaccc ggctcgggaa agacgacgct ggggaggttg atcgcacagt
     1981 acactgatcg cgacttcatc cctttctcgg ccgtaaccga gggcattccg agagtgcgcg
     2041 aggtggtgac ggatgctgag cagcgcctcc agatgggacg gcagacgatc ttgttcgtcg
     2101 acgagatcca ccggttcaac aaggcgcagc aggacgcgtt tctgcctcat gtcgaagccg
     2161 gcacgatcac tctggtgggc gcgacgacgg agaatccttc gttc
//
LOCUS       DBNGSB010000079         5425 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2659133, whole genome shotgun sequence.
ACCESSION   DBNGSB010000079 DBNGSB010000000
VERSION     DBNGSB010000079.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 5425)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5425)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5425
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2659133"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..175)
                     /locus_tag="AC159B_02225"
     CDS             complement(<1..175)
                     /locus_tag="AC159B_02225"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3376603.1"
                     /translation="MKIVRDGVEISYKDEGDGLPLVFLHGFPFSSKMWNRQVAALAPS
                     PRVITPDFRGFGDS"
     gene            complement(256..681)
                     /gene="mscL"
                     /locus_tag="AC159B_02230"
     CDS             complement(256..681)
                     /gene="mscL"
                     /locus_tag="AC159B_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HIR3376604.1"
                     /translation="MLREFKDFVMRGNVVDLAVALILGIAFGRIVSSFVADILMPPVG
                     LVLGGVDFATLFISLDGQRYPTLQAAKAAGAATINYGIFINTIIDFIVVAFVIFLVVR
                     QINRMRRESPTTRACPYCASLISLKATRCPNCTSQVQPA"
     gene            775..2013
                     /locus_tag="AC159B_02235"
     CDS             775..2013
                     /locus_tag="AC159B_02235"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003954 - NADH dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006116 - obsolete NADH oxidation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3376605.1"
                     /translation="MWWMPAPARVLILGGGFGGLYTALVASRLLHDRAEITVIDRNAY
                     FLYTPLLFQVAGGSLQAHHVARSLRHLLPRAVRTIHADVTAIDLEAKQVTAGAGSFSY
                     DFLVMALGGVPNFYGMASAEQHALRFKWLPDVFRLRTHIEQRFAEAGEEPRRARDVLR
                     TVITGAGCTGVELVAELHDWIHGRLLREHPSVPRDAPSLVLAEALDHLLCPMDPNLML
                     KAVRKLLDRRIDLRLERFVTEVGPSWLKLRGPAGEEQVPCGTVIWAAGIKPSALIAGL
                     PLPHGPAGRIDITPALQVVGHPEVLAIGDLAACPDPLGGTLPQTAQVAVQQGPAAART
                     LHALIAGEIPQTFHYRRKGEVVWLGRTGALAEAFGLHLTGLPAWLVSRTVHLARVPDW
                     GDRVAIALQWVKALLAAERR"
     gene            complement(2019..3620)
                     /locus_tag="AC159B_02240"
     CDS             complement(2019..3620)
                     /locus_tag="AC159B_02240"
                     /inference="COORDINATES: protein motif:HMM:NF014683.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015295 - solute:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HIR3376606.1"
                     /translation="MIPAEPWRVAVAALPLAVLLVGLLRLGWRGATASLAGLGTAIAL
                     AIVALATPLSVVGVALYRAAALSLNVLYIIWAALLLYQFAESSGAIRSIGDAVAHLTE
                     DHVLQLLIIGFAFSSFLQGVAGFGVPVAVTAPLLIGLGFAPLEAAAVPLVGHAWAVTM
                     GDMASSFQALLAVTALPPEMLGVWIAGLLGLAAVGSGFAVAHVHAGRRPFRRAFGAIL
                     ALGLSMGGTQFVLALTQHWIIASFGAGMLGLAVSMGMARLARRRPGYLGLLPEWPPPE
                     GRREPPRPAPPAQPEMPFRVAFLPYYVLIVVVGAASVVAPLKRALEGIVLTFAFPAAS
                     TNLGWTTEAASFALSVLGHPGALLIYTTIISVVLFAVIGRRPANWRLIRTGVIEQGVP
                     TTVTILALVGVAMVMTYSGMTFLLAQGLTGTVGGLFPFLSPFLGLLGTVITGSNTNSN
                     VLFGALQRDAATLLKMDPVLMAALQTAGGALGSMVAPAKVVLATVTTGLAGQEGRVMR
                     ITAPYALVLTSLLAIIGVASQLLRR"
     gene            complement(3648..4646)
                     /locus_tag="AC159B_02245"
     CDS             complement(3648..4646)
                     /locus_tag="AC159B_02245"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011144242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR3376607.1"
                     /translation="MRNAEPRLGIVYEHPQWFAPLFAELDRRGVPFDRIDLSRHRFDP
                     MEAVPWTIALNRLSPSAFLRGHGQTIVYGREFLRFLEWRGADVVNNAAAFGLEASKAS
                     QLLLLEALGLRTPRARVINHPAQARDAAEQLKFPVIVKPNVGGSGAGIQRFDTLEQLT
                     ETAATGVVDMGIDGTALVQEFLPARGDHIVRVEVLAGRFLYAIKVYPNFAAGFNLCPA
                     DICQPEEMQAADLCPVDLPKVALKVEGYTPSEAVIRDVLAITRRAGIDLGGVEYLVND
                     RDGEIYFYDVNVLSNFVSDAPSVVGFDPYAVLVDYLVERGRLPQVGGRIAVRAGIS"
     gene            complement(4823..>5425)
                     /locus_tag="AC159B_02250"
     CDS             complement(4823..>5425)
                     /locus_tag="AC159B_02250"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3376608.1"
                     /translation="RPAEAVQEGLSGVIFQQPILFEWLTAEDNVLLPLRLRDPRWWWW
                     PSRRGRYQDSVRQLLRTVELEHFARYYPDKLSGGMQQRVAIARALTLNPQVLLLDEPF
                     GALDEITRDRMNLELLRLYEERGLTILFVTHSIEEAVFLSDRVIVMSRGEFLRTGSSI
                     VREVRVDLPRPRRLALKEDPAFFQKVREGRAALREAQFVE"
BASE COUNT         1083 a         1788 c         1628 g          926 t
ORIGIN      
        1 cggagtcacc aaaccctcga aaatcgggcg tgatcacacg gggcgacggc gccagcgcgg
       61 ccacttgcct gttccacatc ttgcttgaga atggaaagcc atggaggaag acgagtggca
      121 acccgtcccc ctcgtctttg taactaatct caactccgtc tcggacgatc ttcatcggct
      181 atccctcccc ctttgcttac ttcgccatcc gtgctcacaa accgtgcgta ctctccttcc
      241 ggccgaagcg cgtcgtcagg ctggttgaac ctgggacgtg cagttcgggc atcgggtcgc
      301 cttgagggaa atcaacgatg cgcagtacgg acacgcccgg gtcgtcggcg actcgcgtcg
      361 catacggttg atctgtctga cgacaaggaa gatcacgaag gcaacgacga tgaagtcgat
      421 gatagtgttg atgaaaatgc catagttgat cgttgcggca cccgctgcct tggccgcctg
      481 cagggttggg tagcgttgcc catcgaggct gatgaacaga gtggcgaagt ccactccgcc
      541 cagcaccagg ccgaccggtg gcatgaggat gtcggcgacg aatgagctga ctatccttcc
      601 aaacgctatc cccaggatga gggcgacggc gagatcgacc acattcccgc gcatcacgaa
      661 gtccttgaac tctcgaagca tgcttttgtc cctccttcct ctttgctgtc gcttgttcta
      721 tatacccctc gggcatccac agcaacaggc tgcctcttcc cggtctgacg tacaatgtgg
      781 tggatgcccg cgcccgccag agtcctcatc ctcggcggtg ggttcggtgg gctttacacc
      841 gcgctcgtcg cctcgcgctt gctgcacgac cgagccgaga ttacggttat cgatcgcaac
      901 gcctactttc tctacacgcc cctccttttc caggtcgccg gcggctcgct gcaagcgcat
      961 cacgtggcgc ggtccctgcg gcaccttttg ccccgcgccg tacggaccat ccatgctgat
     1021 gtcacggcga tcgatctcga agcaaagcag gtcactgccg gcgcaggatc attctcgtac
     1081 gactttctcg tgatggccct cggcggggtg cccaacttct acggaatggc ctccgccgaa
     1141 cagcacgcgc tgcgcttcaa atggctgccc gacgtctttc ggctgcggac ccatatagag
     1201 cagcggttcg ccgaggcggg agaagaaccg cgccgtgcgc gcgatgtgct gagaaccgtg
     1261 atcaccggcg ccggatgtac cggggtcgag ctcgtggccg aactgcacga ctggatccac
     1321 ggccgtctcc tgcgagagca tccctccgtc ccccgcgacg cgccatccct cgtcctggca
     1381 gaagccctcg atcacctgct gtgcccgatg gatcccaacc tcatgctcaa agccgtccgg
     1441 aaactcctcg atcgccgcat tgatctccgc ctggaacggt tcgtcactga ggtcggaccc
     1501 agctggctga aactccgcgg gccagccggt gaagaacagg tgccgtgcgg aaccgtgatt
     1561 tgggccgccg gcatcaagcc ctcggccctt attgcgggtc ttccactgcc gcacggtccg
     1621 gccgggcgaa tcgacatcac gccggcgctg caggtggtgg gacatcctga agtcctggcg
     1681 ataggagatc tcgcggcgtg tcctgatccc ctcggcggca cccttcctca aacggcacag
     1741 gtggcggtcc agcaaggtcc ggccgctgcg cgaacattac acgcgctcat cgcaggcgag
     1801 attccgcaga cgtttcatta ccggcgaaag ggcgaagtcg tctggctagg ccgcacgggc
     1861 gcactcgccg aagcattcgg cctgcatctc accggcctgc cggcctggct ggtctcgcgt
     1921 accgtgcacc tcgcacgggt gcccgactgg ggtgatcgtg tcgcgatcgc gctgcagtgg
     1981 gtgaaggcgc tgctggcggc cgaacggcgc taacggattc agcgccgcaa aagttgcgat
     2041 gcgacgccga tgatcgcgag gagggacgtc agcactaacg cgtagggggc ggtgatgcgc
     2101 atgacgcggc cttcctgtcc ggcgagtccg gtggtcaccg tcgcgagcac caccttcgcc
     2161 ggcgccacca ttgatccgag cgcaccgcct gcggtctgaa gcgccgccat caagaccgga
     2221 tccatcttca gcaaggtggc cgcgtcgcgt tgcagcgcgc caaacaggac gttcgaattc
     2281 gtgttgctcc ccgtgatgac ggtcccaagc agaccgagga atggcgacaa gaacgggaaa
     2341 agcccaccga ctgttcccgt cagtccctgc gccagcagga acgtcatgcc gctgtatgtc
     2401 atgaccatcg cgacgccgac gagcgccaga atcgtcactg tggtcggcac gccttgctct
     2461 ataacgcccg ttcggatcaa tctccagttt gcgggccttc gtcctatcac ggcaaataac
     2521 acgaccgaaa tgatcgtcgt gtagatgagc agcgcccccg gatgcccgag gacggacagt
     2581 gcgaacgacg cagcctcagt tgtccacccc agattcgtcg acgccgccgg gaacgcgaac
     2641 gtgagcacga ttccctcgag cgcgcgcttg agcggcgcaa ccacgctggc cgcgccgaca
     2701 acgacgatca acacgtaata aggaaggaac gcaacccgga acggcatctc aggttgcgcc
     2761 ggcggcgccg gccgcggcgg ttcgcggcgt ccttccggcg gcggccattc gggaagcaac
     2821 cccaggtacc ctggacgacg gcgcgcgagg cgcgccatcc ccatgctgac cgcgagcccg
     2881 agcatccctg cgccaaagga cgcgatgatc caatgctgtg tcagcgccag cacaaattgc
     2941 gtgccaccca tcgacagccc gagcgcgagg atcgccccga atgcgcggcg gaacggacgc
     3001 cggccggcgt gcacatgcgc cacggcgaat cccgaaccga ccgcagcgag gccgagaagt
     3061 ccggcgatcc agacgccgag catttccggg ggaagcgcgg tgaccgccag cagcgcctga
     3121 aacgaggacg ccatgtcgcc catcgtcacc gcccatgcgt gaccgaccag gggcacggcc
     3181 gccgcttcga gcggggcgaa tccgagcccg atgaggagcg gcgccgtcac tgccaccggg
     3241 acgccgaagc ccgccacgcc ttgaagaaat gagctgaacg cgaatccgat gatgagcagc
     3301 tgtaggacgt gatcctccgt gagatgcgca acggcgtcgc cgatactacg gatcgcaccg
     3361 ctcgattcgg cgaattgata cagcagcaat gcagcccaga tgatgtagag gacgttgagg
     3421 ctgagagcgg cggctctgta taaagcgacc ccgacgacgc tcagcggtgt ggccaacgca
     3481 acgatggcga gggcgatcgc agtcccgagt ccagccaggc ttgccgtcgc gccccgccat
     3541 ccaagacgaa gcaaccccac gagcagcacc gcgagtggca gtgccgcgac agcgactcgc
     3601 cagggttctg ccggaatcac cggcccctcc tggcagacgc tagcccatca agaaatgcct
     3661 gcccgcacgg cgattctccc acccacctgc ggtaggcgtc cacgctcaac caagtagtcg
     3721 accagcaccg catacggatc gaatcccacc acagagggtg catcgctcac aaaatttgag
     3781 agcacgttga cgtcgtagaa gtagatttcg ccatcgcgat cgttaaccag gtattcgacc
     3841 cctccgagat cgattccagc gcggcgcgtg atggccagca catcgcggat cactgcctcg
     3901 gacggcgtat agccttcaac tttgagcgca acctttggaa gatcaaccgg gcagagatcc
     3961 gccgcctgca tctcctccgg ctgacagatg tccgcggggc acagattgaa tccggccgcg
     4021 aagttcggat agaccttgat agcataaagg aaacgaccgg caaggacctc gacccgaacg
     4081 atgtgatccc cgcgggcggg caagaactct tgcacgagcg ccgttccatc gatgcccatg
     4141 tcaaccacgc ctgttgcagc cgtctccgtg agctgctcca aagtgtcgaa tcgctgaatg
     4201 ccggcaccgc tgccgccgac gttgggtttg acaataacgg gaaacttcag ctgctcggcg
     4261 gcatccctcg cctgagcggg gtgattgatg accctcgcgc gaggcgtgcg gagtcccagc
     4321 gcttccaaca gcagcagctg ggacgccttc gaggcctcca gcccaaatgc ggccgcattg
     4381 ttcaccacat cggcgccgcg ccattcgaga aatctgagaa attcacggcc gtagacgatc
     4441 gtctgcccgt gcccacgcag aaacgctgag ggactgaggc gattcagcgc gatggtccac
     4501 ggcaccgcct ccatcggatc gaaccgatgc cggctgagat cgatccgatc aaatggcaca
     4561 ccgcggcggt ccaactcggc aaacagtggc gcaaaccatt gtgggtgttc gtatacaatg
     4621 ccaagacgcg gctctgcatt ccgcatgtcg tactcctctc aattttagaa gtgtcaggga
     4681 aaagctggaa aggcggaggc tatgccctcc gccggggaca gatgcagccg cgcgggagtt
     4741 gcagcaggaa gaagttgcct gcgttcatcg ttgccgtcaa tcctaacaga cccttccctg
     4801 tcgaacaatg ggacgggata gtctactcca caaactgcgc ttctcgaagt gctgcccggc
     4861 cctcgcgcac tttctgaaag aatgccggat cctctttgag cgcaaggcgc cgaggccgcg
     4921 gaaggtcgac gcgcacttca cgcacgatgc tggagccggt ccgtaagaac tcgccgcgac
     4981 tcatgacgat gacgcggtcc gagaggaaca ccgcttcctc gatcgagtgc gtcacgaaca
     5041 gaatcgtcag gccgcgttcc tcgtacagtc gaaggagctc gaggttcatc cggtcgcgcg
     5101 tgatctcatc gagcgcgcca aacggctcgt cgagcaggag tacctgaggg ttgagggtca
     5161 aggcgcgggc gatggccaca cgctgctgca tgccgcccga aagtttgtca ggatagtacc
     5221 tcgcgaaatg ctcgagttcc accgtgcgca gcagctgccg cacgctgtcc tgataacggc
     5281 cgcggcgcga gggccaccac caccagcgcg gatcccgcaa cctgagcgga agcagcacgt
     5341 tatcctctgc cgtcagccac tcaaagagga tcggctgctg gaagatgaca cccgacagcc
     5401 cttcctgaac agcctcagcc ggtct
//
LOCUS       DBNGSB010000080         2640 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3428541, whole genome shotgun sequence.
ACCESSION   DBNGSB010000080 DBNGSB010000000
VERSION     DBNGSB010000080.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3428541"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /gene="pyk"
                     /locus_tag="AC159B_02255"
     CDS             complement(<1..1012)
                     /gene="pyk"
                     /locus_tag="AC159B_02255"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HIR3376609.1"
                     /translation="MKIPEAPRDWDRRTSIIATFGPASNSPERIRELIEAGVDVVRLN
                     FSHGTADEHARIFELVRKAEAGVGKPVAIMQDLAGPKLRVGDLQGGKVHLREGERLTI
                     GTRKSARAAHRITTTYPRLARDVRKGDRILLDDGTLELVVEDTGNGAVTTRVVRGGTL
                     TEHKGLNLPGIALQVPVITEKDLRDLESGVRLGVDYVAFSFIQRGRDVELGRRAVNRH
                     RARIPIIAKLEKAEAIKSLDEILKAADGVMVARGDLGVELDPERVPVLQKTIIQKANE
                     AGLPVITATQMLESMVDSPRPTRAETSDVANAIIDGTDAVILSAEPSIGKYPVEAVRL
                     MYR"
     gene            complement(1226..1657)
                     /locus_tag="AC159B_02260"
     CDS             complement(1226..1657)
                     /locus_tag="AC159B_02260"
                     /inference="COORDINATES: protein motif:HMM:NF016089.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR3376610.1"
                     /translation="MAGQPMTLRDWALLILRLGIGITFIAHGYSKVFPSGPGGFAGFV
                     QSLGFPGPLFFAWVVAITEFFGGIAMVIGFMVRFVGPLMVIEMIVTTVRVKVAGGVGF
                     IEMQRTGWELDFLLLCVAAALALTGAGALSLDAALRSRRTA"
     gene            complement(1945..2349)
                     /locus_tag="AC159B_02265"
     CDS             complement(1945..2349)
                     /locus_tag="AC159B_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376611.1"
                     /translation="MPGNEARIAVEIVHLELYIVPRKHRAAFRKSLQEPIRIAQKITH
                     AGGGSGPYFYRVEPQRARRGTPQIGTILRVKPNEDLWVEIAFYRDWRRRRSILRKIWG
                     NPKFVASVGVSEGLVSRRPNAWGVANAALQEL"
BASE COUNT          520 a          802 c          762 g          556 t
ORIGIN      
        1 tgcggtacat gaggcgcacc gcctcaaccg ggtacttacc gatggacggt tctgcggaca
       61 gaatgaccgc gtcggttcca tcgatgatcg cgttggcaac gtcagatgtt tcggcacggg
      121 tcggccgagg gctgtctacc attgattcca gcatctgggt cgccgtaatc acggggagcc
      181 ctgcctcgtt ggccttctgg atgatcgtct tctgcaagac cgggacacgt tcggggtcga
      241 gctccacgcc gaggtcgccg cgggcgacca tcaccccgtc ggccgccttg agaatctcgt
      301 cgagggactt gatcgcctcg gccttctcca gtttcgcgat gatcggaatg cgtgcacggt
      361 gccggttgac agcgcgccga cctagttcga cgtcgcgccc gcgctggata aagctgaacg
      421 ctacgtaatc gacgcccagg cgcacgccgg actccaagtc tcgcagatcc ttctcggtaa
      481 tgacgggaac ttgaagcgcg atccccggca ggttgaggcc tttgtgttct gtcagggttc
      541 cacctcgtac cacgcgggtt gtgaccgcac cattaccggt atcctccacg acgagttcta
      601 gcgtgccgtc gtcgagcagg atccggtcgc ccttccggac gtcgcgcgcc agccggggat
      661 acgttgtggt gatcctatga gctgcgcgcg cacttttccg agtcccgatc gttagtcgtt
      721 caccttcccg caggtgaact ttgcctccct ggagatcgcc gacccgcaac tttggcccgg
      781 ccaaatcttg catgatggcg acaggcttgc cgacgcctgc ttcggcctta cgcaccaatt
      841 cgaagatcct ggcgtgctcg tcggccgtcc catgggagaa gttgaggcgg acgacatcga
      901 cgccggcctc gatgagctct cggatgcgct ctgggctgtt gctggcggga ccaaaggtgg
      961 cgatgatcga ggtgcggcga tcccagtcgc ggggcgcctc cggaattttc atcttaaccc
     1021 ggcaccgagc aaaattcacc ggcctcgctg cagttctcca gactcaatca acgtgcaaaa
     1081 ttcaccggcc tcgccccaag ctcggcctac tcaacagtgc gccgatgaac ctcaacagaa
     1141 cactggtggg cgccacctga ttacgacggc gtacccgctt cgtcgcacca aattgaggtc
     1201 cttggcgctt acagcgttct tgttatcagg cggtccgccg cgatctgagc gcggcatcca
     1261 gcgatagtgc gccggcaccc gtgagtgcca gcgccgcggc gacacacaac aggaggaagt
     1321 cgagttccca ccccgttcgc tgcatctcga taaagcccac gcctcctgcc actttgacgc
     1381 ggactgtggt gacgatcatt tctatcacca tcagcggtcc tacgaacctg accatgaatc
     1441 cgatgaccat ggcgataccg ccgaagaact cagtgatcgc cacaacccag gcaaaaaata
     1501 gcggcccggg aaagccaaga ctttggacga atcccgcgaa cccgcctggg cccgacggga
     1561 acaccttgga ataaccgtgc gcgatgaacg tgatcccaat accaagacga aggatgagta
     1621 gtgcccaatc tcggagcgtc attggctgac cggccatcgt tctcacctcc gattgtttcc
     1681 tgtacattcc gggatttttg taacagtgtc gcaacaggcg agtcgttcag gaaaagcaaa
     1741 cagccaagct aaccagctcg gcatgaaagt ggagccggcg atcggattcg aaccgatgac
     1801 ctgcagatcc tataaaaccc cgcatcaatc gagcgaaagc acaggtcaat gggggtgtgc
     1861 gaaaggactt ctttccgttc agcctccgtc gggacccgga cgggagtcgc tcataagttg
     1921 gtgctgtgct ctgattatga cccttcagag ctcctggagg gcggcgttgg ccacccccca
     1981 tgcgttcggg cgccgcgaga ccaagccttc agagactccg acggaagcaa cgaacttggg
     2041 attgccccag attttcctca ggatagatcg cctcctgcgc cagtcccggt agaaggcgat
     2101 ctcgacccag aggtcttcat tcggttttac tctcaggata gtcccgatct gaggcgtgcc
     2161 acgacgcgcg cgctgaggct cgactcgata gaagtaaggg ccgcttccgc cgcctgcatg
     2221 cgtaatcttc tgggcgatcc ggataggttc ctgaagactc ttgcgaaatg cggcgcggtg
     2281 tttccttggg acaatataga gctcgagatg gacgatttcg acggcgattc tcgcctcgtt
     2341 tccgggcatg gccgatcatc tatcgtcgga tatttgtgat ttgcaagttc gcccatggct
     2401 tatgtgcgac ctcccatgcc gaatcaagga agggcgttct aatgcaatat atgctccaag
     2461 accgtccctg gccgcctact ctgcacctgt ctgattgcgg ccagcagttc tttcagtaga
     2521 tttgcgctgc gacggcatac ttgctcgttc gtgggatgag cgtggggcca cctgctgctt
     2581 gcgtcgggta gtagcataca gtagcataag caacgtctgg aatggggttt actgcgggtg
//
LOCUS       DBNGSB010000081         1796 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_669163, whole genome shotgun sequence.
ACCESSION   DBNGSB010000081 DBNGSB010000000
VERSION     DBNGSB010000081.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_669163"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="AC159B_02270"
     CDS             complement(<1..280)
                     /locus_tag="AC159B_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl cyclase domain-containing protein"
                     /protein_id="HIR3376612.1"
                     /translation="MGIDETGTLARLKALRREFIEPLITKYHGRIVKLMGDGVLVEFA
                     SVVDAVTCAVEIQRLIAEQGAGEPQDSQVVFRIGINLGDVIIDGDDVYG"
     gene            complement(603..>1796)
                     /locus_tag="AC159B_02275"
     CDS             complement(603..>1796)
                     /locus_tag="AC159B_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018097204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR3376613.1"
                     /translation="MIRMLIVGYVFAIRSERLLCREVQVNLAYRWFCGLSIEDKIPDH
                     SAFSRARNERFRDSNIFRRVFERIVKACISAGLVGGEGFAVDASLIVADANKQRSIPG
                     SEWEKTRDRETASRAVKEYLATLDDAAFGAASDVTPKFISPSDPAAQWTGAMRGPAFF
                     AYANNYLVDVKFGVIVDVEASRAIRQAEVGAAKTMIGRTEERFGLKPEHLAADTAYRS
                     AETLNWIVNEKKIAPHIPVIDKSKREDGSLSREDFSFDQDGNVYVCPQGKLLHTTGRI
                     HDGETLLYRARTSDCGPCPLKPKCCPKAPERKIPRSIYEDARDAARALVDTEAFKQSH
                     RDRKRVEMLFAHLKRILKLGRLRLRGPCGAQDEFTLAAIAQNLRRLAKLVARPPPPAA
                     AACAA"
BASE COUNT          359 a          540 c          526 g          371 t
ORIGIN      
        1 caccatacac gtcatcgcca tcaataatca cgtcgccaag attgatccca atgcgaaaaa
       61 cgacttgtga atcctggggc tcgcctgcgc cttgttccgc tatcaaccgc tggatctcca
      121 cggcacaggt cacggcgtcg acaacgctgg caaactccac tagcaccccg tcacccatca
      181 gcttgacgat acgtccgtgg tatttggtga tgagcggctc gataaattcg cggcgaagcg
      241 ccttcaaccg ggcgagcgtg cccgtttcgt cgatacccat aagccgactg taccccacca
      301 catcggcggc gaggatcgcg gctagctttc ggtcgacctg ctgcgactgg ttcacgctgc
      361 ggaataatag ggaaaactgc aagcctaggc cacggtttag ctctcaattg atagtgacaa
      421 ttttgaagat tcctgatgac tttgggtatt cttctcggaa tgtctgcttc tggcagattt
      481 tgttgcagaa gttggcaatc agagggccga cgcagctgta gcgattttct tgaagcgtcc
      541 cgtcaccacc cgccgcattg gaagcggcgg ctttgatgca gtggcactga cgcccgcaac
      601 ggttacgcag cacacgcggc ggccgccggt ggtggtggtc gggcgaccag tttggcgagc
      661 cggcgtaggt tctgtgcgat ggctgcgagc gtgaactcgt cttgcgcgcc acatgggccg
      721 cgcaaccgga ggcgaccaag cttcaggatg cgcttgagat gggcgaacag catctcgacg
      781 cgtttgcggt cgcggtgcga ttgcttgaac gcttcggtgt ccacgagtgc acgggcggca
      841 tcgcgagcgt cttcgtaaat gctgcgcggg attttgcgct ccggcgcctt tgggcagcat
      901 ttgggcttca gcggacaagg gccacaatca gacgtccttg cgcgatagag gagagtctcg
      961 ccatcgtgga tcctgcctgt cgtgtgcagg agcttgccct gcgggcacac atagacattg
     1021 ccatcctggt cgaagctgaa gtcctcccgg ctcagtgagc cgtcctctcg cttggactta
     1081 tcaatgacgg gaatgtgcgg agcgatcttc ttttcgttga cgatccagtt gagcgtctcc
     1141 gctgagcggt aggcagtgtc cgctgcaaga tgttcgggct tcaggccgaa acgctcctcg
     1201 gtgcggccaa tcatcgtctt cgccgcgccg acctccgctt gacgaatggc gcgagaggcc
     1261 tcgacgtcga caattactcc gaacttcaca tcaaccagat agttgttggc gtaggcgaaa
     1321 aatgctggtc ctcgcatcgc gccggtccat tgggcagccg ggtcggacgg cgagatgaac
     1381 ttcggagtga cgtcgctcgc cgcaccgaac gccgcatcat cgagggttgc cagatactcc
     1441 ttcacggcgc ggctcgcggt ctcgcgatca cgcgtcttct cccattccga gccgggaatc
     1501 gaacgctgct tgttggcgtc cgccacaatc aagctcgcat cgaccgcgaa cccttcaccg
     1561 ccgaccagcc cggccgaaat acatgcctta acaatacgct cgaagacccg gcggaaaatg
     1621 ttgctgtcac gaaagcgctc attacgggcg cgcgagaatg ccgaatgatc cgggatcttg
     1681 tcctcgatgc tcaagccgca gaaccagcgg tacgccaaat tgacctggac ctcacggcag
     1741 agcagccgct ccgaccggat agcgaaaaca tagccgacga tgagcatccg gatcat
//
LOCUS       DBNGSB010000082         4674 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3123084, whole genome shotgun sequence.
ACCESSION   DBNGSB010000082 DBNGSB010000000
VERSION     DBNGSB010000082.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4674)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4674
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3123084"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..941
                     /locus_tag="AC159B_02280"
     CDS             195..941
                     /locus_tag="AC159B_02280"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3376614.1"
                     /translation="MPETSDGCLLVHGFSGSPLEMVPLGEALSEAGWTVVIARLAGHG
                     TSPKDLAGTKWRDWVASAATAYEDLRRRTRRQAIIGLSMGGAIALYLAAREGADAVVA
                     ISTPIRVRPMLAGAARVASRVIPLAPVIFRLSPRDPMARQYHSPYAQIPLGATAELSA
                     LLSETVRSLASVTAPLLVVQGRRDWVIPKDSGEEILSRATHAQARLLWLRRSGHMATL
                     DRDREMLFEEVKGFLRAHLVGESPVDGTAD"
     gene            925..1491
                     /locus_tag="AC159B_02285"
     CDS             925..1491
                     /locus_tag="AC159B_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376615.1"
                     /translation="MEQQIERSKVVVRWIVAATLFGAVAYLKRTGGVAIPWSVVIALT
                     AVVAALNLIYWSILRRSAPRWLKYVTTATDLALISLLVLFTGGSGSVFHVAYYVVLVS
                     NSIRYGMGMALYVAIVYNITYVAMLAFAPPTGDLTTEAVKILAFWGVALYAGYLAMRF
                     QRYARILESYEETIARLRAELGGPRPAK"
     gene            complement(1691..2383)
                     /locus_tag="AC159B_02290"
     CDS             complement(1691..2383)
                     /locus_tag="AC159B_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR3376616.1"
                     /translation="MATKLTDAVIWLFALVAVALFRIIDSTLDLRIFGHEHVADVLRT
                     KRPFLVVVWHGRGLLPIFFFQGYPLVVYSSQSRAGIAPALSRYVRKLTLASLRHLGYQ
                     VLDASTFHSESRGVIRFLQYLERGSGGLIAADGPDGPMFRAKPGAAYVAKKTRVALLP
                     IAAAIRDAIALDSWDRFEIPRPFSKAALAIGPALDVPEDISEEALARLSTNLEVVLND
                     LTARAEVEAFAV"
     gene            complement(2373..4160)
                     /locus_tag="AC159B_02295"
     CDS             complement(2373..4160)
                     /locus_tag="AC159B_02295"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015645 - fatty acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HIR3376617.1"
                     /translation="MTVETLRDLFEHIVTTNPAGKELLRIRAGRDWRTLTVKEFEEVT
                     RATARRLAQAGIGHGDRVALFSENRPEWHIIDFACHLLGAAPVPIYPSVPAHQVRYIV
                     ADSGSKMLLVSGKERAQAAITAIQELPSVRLVAIDPNLAGLPSLDDLPLREGGQAMSP
                     LKPADLASVIYTSGTTGDPKGVMLTHRNLASQITSIKPRYPITSDDDVMSFLPLSHIF
                     QRILDYIFLYSGCRVTYVDAVEKVAAMLPLVRPTIMASVPRVYERAYVQVMTRMQREK
                     PIKRRLFNWALRAGRRVKEAEWKGVRASPLARLQYSLARKLVFSKILERFGGRLKFSV
                     SGGAPLAREIAEFFDIVGLPILNGYGLTESSPVIAVNRLEANRLGSVGEAAPDVEIKI
                     APDGEILARGPNIMKGYWNKPEATAEAVDAQGWLHTGDIGHLDKDGFLFITDRKKDIL
                     VTAGGKNVAPQPIEARLATSPYIAQAILIGDRFPYCSALIVPNFENLSAYLKEQGVNG
                     NLSTESVAKHPVTEALVAAAVKETNEPLAAYERIRRFTVLPRELSLEQGEITPTLKVR
                     RRIVSERFKALIEEMYLKTHRTAQFALGD"
     gene            complement(4250..>4674)
                     /locus_tag="AC159B_02300"
     CDS             complement(4250..>4674)
                     /locus_tag="AC159B_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376618.1"
                     /translation="NGQPTSNGIRPAEGIGAVQLGMTPAQVQEAIGLPPSSRTANGWY
                     DWDSRKLSVRFDRGVAIVIYTEDPAAGTAEAIRLGSTDVDLIKTYGAPACASLFSGSG
                     KATLGWVYHGMVVFLNGSPRQIYALAVVPKGFATAVCR"
BASE COUNT          857 a         1418 c         1469 g          930 t
ORIGIN      
        1 tgtgaccggt cggcttcaag tcagccagct ggaaactggt gatggtggta agcgtacctc
       61 gactgaagta atcgcggatc aagttgtctt cctggacgcg cctcgtaagg cggcgccggc
      121 ggaaacgtga cagggtttgc aaaagggttc ctttgccagg agacgttgtg caatgacgcg
      181 aaaaccgcga ggcgatgccg gaaacatcgg atggatgcct cctagtacac gggttttccg
      241 gttcccccct ggaaatggtg ccgctcggtg aggcgctctc agaggcgggg tggacggttg
      301 tcatcgcacg gctggccgga cacgggacgt cgccgaagga cctggccggt accaagtggc
      361 gcgactgggt cgcatcagcc gcaaccgcct atgaggattt acgccgccgc acccgacggc
      421 aggcaatcat tgggctgtcc atggggggcg ccatcgcgct gtatctcgct gcaagggagg
      481 gcgcggacgc agtcgtcgcg atctcaacac cgattcgggt aaggccgatg ctggcgggag
      541 ccgcccgcgt cgcttcacgc gtcatcccgc tcgccccggt gatcttccgc ctcagcccac
      601 gggatcccat ggcccgacaa tatcattcac cctacgccca gatcccactt ggggccaccg
      661 cggaactctc ggcgcttctc agcgagaccg tgcgctcgct tgcatctgta accgcgccgc
      721 tgcttgtggt tcagggccgc cgggactggg tgatcccaaa agacagcggg gaggagatcc
      781 tctcgcgggc cacccacgcg caggccaggc tactctggct acgacgttcg ggacacatgg
      841 ccacgctcga tcgagatcgg gagatgctgt tcgaagaagt aaaggggttt ctccgcgccc
      901 acctagtggg tgagagtccc gtcgatggaa cagcagattg agcgaagcaa ggttgttgtg
      961 cggtggatcg tcgccgccac gctctttggc gccgttgcct acctgaagcg aaccggcggc
     1021 gtcgcgattc cgtggagcgt tgtgatcgcg ctcactgccg tcgttgccgc actcaacctc
     1081 atctactgga gcatccttag acgcagcgct cctcggtggt tgaaatacgt gacgacggca
     1141 accgatcttg cgctgatctc attgctggtt ttgtttacgg gaggcagcgg aagcgttttc
     1201 cacgttgcgt actatgtcgt cctcgtcagc aacagcattc gctatggtat ggggatggcg
     1261 ctgtatgtcg ccattgtcta caacatcaca tacgttgcga tgctggcgtt tgcgccgccg
     1321 acaggcgacc tgaccaccga ggccgtcaag attctcgcgt tctggggtgt ggcgctgtac
     1381 gccgggtacc tggccatgcg cttccaacgc tatgcaagga tcttggaatc ctacgaggag
     1441 acgatcgcga ggctgcgcgc cgaactcggc ggaccaagac ccgcgaaatg atggcccccc
     1501 gccccgcaca acgaaccctc acccgccgcc acgtcgcgtg gagcagtacg ctgggcttga
     1561 ttctcctact gttgctcggg gggatagtct ggaaagctac caacgcgctg tggttcctgc
     1621 tgtttccatt actcttggtc gggctcgtca ccgcagctgc gaccgcggtg ttgtgggggc
     1681 tccgacattt ctaaacggca aacgcctcta cttccgcccg cgcagttaga tcgtttagca
     1741 ctacctcgag gttggttgag agtcgtgcca gtgcttcttc actgatgtcc tcaggcacat
     1801 ccaatgccgg accgatggcg agggcggcct tcgagaatgg cctcgggatt tcgaaccgat
     1861 cccaggaatc caacgcaatc gcatcgcgga tagccgcggc gatcggaagc agcgcgaccc
     1921 gcgtcttctt cgctacatag gcggcccccg gtttggcccg gaacatgggt ccgtcagggc
     1981 catcggcggc gatcaaccct ccactcccac gctcgaggta ttggaggaag cggatgacac
     2041 cccgagactc gctatggaac gtggacgcat cgaggacttg ataaccgaga tggcgcagcg
     2101 atgccagcgt cagcttccgg acatatcggg acagcgcggg ggctattccc gctcgtgact
     2161 ggctggaata cacgacgagt ggatagccct ggaagaagaa gatcggcaga agcccgcgtc
     2221 cgtgccacac gacgaccagg aacggtcgtt tggtgcgcag gacatcagcg acgtgctcgt
     2281 gaccgaagat ccgcagatcg agcgtgctgt caatgatccg gaaaagggcg accgcgacca
     2341 gggcgaagag ccagatgacc gcgtcggtca gcttagtcgc caagcgcgaa ctgagccgtc
     2401 cggtgagtct tcaggtacat ttcctcgatc agggctttga accgttctga gacgatgcgc
     2461 cggcgtactt tgagcgtcgg agtgatctct ccctgttcga gagagagttc gcgcggcagc
     2521 actgtgaagc ggcgaatccg ctcgtacgcc gccagcggct cgttggtttc tttcaccgct
     2581 gcggcgacga gggcttccgt gaccggatgc ttcgccacgg actcggtgct gaggttgccg
     2641 ttcactccct gctctttgag gtacgccgac agattctcga aattcgggac gatgagagcg
     2701 ctgcagtacg gaaatctgtc cccaataaga atcgcctgcg caatgtaggg actcgtggcg
     2761 agccgcgcct cgatcggctg cggggcgacg tttttccccc cggcggtgac cagaatatcc
     2821 ttcttccggt cggtgataaa cagaaagccg tctttgtcga gatggccaat gtcgccggta
     2881 tgcaaccagc cctgcgcgtc cacggcctcc gctgtcgcct caggcttgtt ccagtatccc
     2941 ttcatgatgt tgggtccgcg tgcgaggatc tcgccgtcgg gcgcgatctt gatctccacg
     3001 tcgggcgcgg cctcgcccac cgagccgagc cggttggctt ccagacggtt caccgcgatg
     3061 accggcgagc tctcggtcaa cccgtatccg ttgaggatcg gcagccccac gatatcgaaa
     3121 aattccgcaa tctcacgcgc gagcggggcg cctccggaaa cggagaactt caacctcccc
     3181 ccaaatcgtt ccaggatctt ggagaatacg agcttgcgcg ccagactgta ctgaagccgc
     3241 gcgagcggcg acgcgcggac gcccttccac tctgcctctt tcacccgtcg gccggcgcgc
     3301 agggcccagt taaatagtct gcgtttgatt ggtttctccc gctgcattct tgtcatgacc
     3361 tgaacgtaag cgcgctcata cacccggggg actgaggcca tgatcgtcgg gcgaaccaga
     3421 ggcagcatcg cggcgacctt ttcgaccgca tcgacatacg tcacgcggca tccagaatac
     3481 aaaaagatgt aatcaagaat ccgctggaag atatgcgaga gcggaaggaa cgacatgacg
     3541 tcgtcgtcgc tggtaattgg gtacctgggc ttgatgctgg tgatctgtga ggcgaggtta
     3601 cggtgcgtga gcatgacgcc tttcggatcg ccggtggttc cggatgtgta gatgacgctg
     3661 gcgaggtctg cgggcttcaa tggagacatt gcctggccgc cttcgcgcag cggcagatcg
     3721 tccagagagg gaagcccggc gagattcggg tcgatggcga cgaggcgcac gctgggcagt
     3781 tcttggatgg ccgtaatcgc cgcctgggcg cgttctttcc ccgataccag tagcatcttc
     3841 gatcccgagt ccgcgacgat gtaccgcacc tgatgtgcgg ggacggaggg ataaatggga
     3901 accggggcgg cgcctaagag gtggcaggcg aagtcgatga tatgccactc gggccggttt
     3961 tcggagaaca gggccacgcg gtcgccgtga ccgatgcccg cctgcgccaa ccggcgtgcc
     4021 gttgctcgcg tgacctcttc gaattccttc accgtgaggg tccgccagtc acgaccggcg
     4081 cgaatgcgca gaagctcctt gccggctgga ttggtggtga cgatgtgctc aaataggtcg
     4141 cgcaaggttt ccacggtcat ccctcgcttt gaagttactg acatcggcca ggggaagcgc
     4201 cgctgatgtt ctggaggtgc ggcgcatccc ctgccgttct tccacccgtt tacctgcaga
     4261 ctgcggtcgc gaaccccttc ggtaccaccg ccagcgcgta gatttggcgg gggctgccgt
     4321 tgagaaacac gaccattccg tggtacaccc aaccgagggt tgctttgccc gatccggaga
     4381 acagcgatgc acacgccggc gcgccgtagg ttttgatcag atccacatcg gtggatccaa
     4441 gccggatggc ttccgctgtc ccggccgccg ggtcttccgt atagataacg atggccacac
     4501 cgcggtcgaa ccgcacgctc agcttgcgac tgtcccagtc gtaccatcca tttgcggtgc
     4561 ggctactcgg cgggaggcca atggcctcct gtacttgtgc gggtgtcatc ccgagctgta
     4621 ccgcgccgat cccttcggcg ggacggatgc cgttactcgt cggttggcca ttgc
//
LOCUS       DBNGSB010000083         6519 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3277764, whole genome shotgun sequence.
ACCESSION   DBNGSB010000083 DBNGSB010000000
VERSION     DBNGSB010000083.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 6519)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6519)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6519
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3277764"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="AC159B_02305"
     CDS             complement(<1..794)
                     /locus_tag="AC159B_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR3376619.1"
                     /translation="MSSDTLSDVLRAIRLQGAVYFFVEAAAPWVAQAPPAKAVAAAVM
                     PGSEHVIEYHVVTRGACWAGLLGGEAARLEAGDVIVFPHGDAHVVSSTPGMRAEPDWE
                     AHRRPADEQLPFFYREGGEGGDKIQLVCGFLGCDARPFNPLLSTLPPMIHVHGAADDS
                     GLGQFIRLAIDESHQKRLGGECVLARLSELMFVEVVRRYLESLPEEHAGWLAGLRDRH
                     VGRALSLLHGDPAHPWTLEGLAQRAGLSRSALAERFAHFVGQPPMQ"
     gene            989..1822
                     /locus_tag="AC159B_02310"
     CDS             989..1822
                     /locus_tag="AC159B_02310"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3376620.1"
                     /translation="MTTTVLQCLPTVLDFAAIKARQQATWASGDYSIIGATLQIVAES
                     LCEAIDLRAGERVLDVAAGPGNAAIAAARRWCEVVATDYVPALLERGYERAAAEGLTV
                     DFREGDAESLPLPDASFDVVLSTFGGMFAPDHSRVARELLRVCRPGGRIGLANWTPEG
                     FIGQVFKTVGKYVPPPAGLKSPALWGTEDWLREIFGDQITTLKATRKHFVFRYRSAQH
                     WLETFRTYYGPILKAFAALAADKQDALAADLIALAQRSSNSADTFAAPSEYLEVVITK
                     R"
     gene            1903..2160
                     /locus_tag="AC159B_02315"
     CDS             1903..2160
                     /locus_tag="AC159B_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376621.1"
                     /translation="MHTSIYQEPRQSTASFLTTGGAKWATGIIAGSLAAAVGFMLGIT
                     AAASTTTSKEPSTPDTIPAFVYFPPQYVNAATEIDELPPQF"
     gene            2178..2717
                     /locus_tag="AC159B_02320"
     CDS             2178..2717
                     /locus_tag="AC159B_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3455 domain-containing protein"
                     /protein_id="HIR3376622.1"
                     /translation="MCKPSKVTIFLLVLHLGGCAAPELSEPLADVPDKLKPSANESLA
                     MIVPAKGVQIYECRARKDQPGAYEWAFVAPEADLFDVRGNRIGRHYAGPHWESNDGSK
                     IVGTVKERADASVADAIPWLLLAAKSVGSEGSFSKVTSIQRVNTVGGVAPKAGCSQAA
                     AGTPARITYTADYYFFTAK"
     gene            complement(2912..3325)
                     /locus_tag="AC159B_02325"
     CDS             complement(2912..3325)
                     /locus_tag="AC159B_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HIR3376623.1"
                     /translation="MLLVDTNVLIDVLEDDADWAEWSLKQLRALAQVHDLIINPVIYA
                     ELSLAFKTVEALDAAVEKMGLVLRELPKPALFLAGKAFVRYRREGGKKSNGLADFFIG
                     AHAAVSGCPILTRDARRYSAYFPRVVLITPDAARH"
     gene            complement(3325..3561)
                     /locus_tag="AC159B_02330"
     CDS             complement(3325..3561)
                     /locus_tag="AC159B_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010636975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0001558 - regulation of cell growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIR3376624.1"
                     /translation="MPTTLTSKGQVTIPKKIRDALKLVPGASVEFAVNGDGDVVLRKV
                     GARKSSKPDRFAAVRGKAQVKWRTDELMALLRDE"
     gene            3850..4239
                     /locus_tag="AC159B_02335"
     CDS             3850..4239
                     /locus_tag="AC159B_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020631677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HIR3376625.1"
                     /translation="MSVRRFTSNDAKFYQYLDREIFVGDVLDSSNSDTMSVGFYRNKK
                     KGEKNEWIVTYDEALIVTKGALTIRFDEGAKTAKVGEVIFLTKGTKIAYEAGEDDTEV
                     VYVSYPHWMDAQKKSKHAHLLDSYHPV"
     gene            4320..5135
                     /locus_tag="AC159B_02340"
     CDS             4320..5135
                     /locus_tag="AC159B_02340"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007353752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase family protein"
                     /protein_id="HIR3376626.1"
                     /translation="MGFDLDAYLERIGCLGERAPTLATLAVIQLRHTEAIAFENLNPL
                     MGWPVRLDMESLQEKIVRDGRGGYCYEHNLLFKHALEALGFGVKGLLARVLWSAPKGA
                     ITPRSHMVLQVDLDGEPYIVDVGFGGVTLTGPLRLIADIEQATPHEPFRLRRVDDEFI
                     EEVKIRDAWIPLYRFSLQEAFLPDYEINNWYTSTHPDNLFVNELLAARPAPNRRYALR
                     NNQFTVHHLTGRTDRRILANADEMREILEGPFGLTLPDARELDATLKRLTASS"
     gene            5250..>6519
                     /locus_tag="AC159B_02345"
     CDS             5250..>6519
                     /locus_tag="AC159B_02345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005390963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR3376627.1"
                     /translation="MKQLQKAQIDEFKAHFGGEVLLSGEAGYDEVRQIWNAMIDRRPA
                     LIVRCASPEDVVQAVKFARKHNLLVSIRGGGHNIAGNAVCDDGLMIDLSLMKSVEVDP
                     NARRAMVEPGCTLADFDAATQAHGLATPLGINSTTGVAGLTLGGGFGWLSRKYGMTID
                     NLLSADVVTADGNQVHASETENADLFWGLRGGGGNFGIVTSFEFHLHPVGPDVLTGLI
                     AFPFAQAKSVLKQFARFTETMPDELNVWMVTRKAPPLPFLPEDVHGKEIVVLPLCYAG
                     DPREGEKLIEPLRGFGTAHGEHIGVQPYTAWQQAFDPLLTRGARNYWKSHNFSQLSDG
                     AIDTIIEYAGTLPSPQCEIFVGTIGGQTARVAPEAMAYSSRDANYVLNVHARWESAAE
                     DKRCIAWAREFFAKSQPFASGGAYINFLTQD"
BASE COUNT         1331 a         2047 c         1954 g         1187 t
ORIGIN      
        1 tactgcatcg gcggctggcc gacgaagtgg gcgaagcgct ccgccaaggc tgagcgcgat
       61 aggccggccc gctgcgcgag cccctccagg gtccaagggt gcgccgggtc gccgtgcagc
      121 agcgacagcg cgcggccgac gtggcggtcg cgcaggcccg ccagccagcc agcgtgctcc
      181 tcgggcaagg attccagata gcggcgcacc acctccacga acatcagctc ggagaggcgc
      241 gccagcacgc actcgccgcc gaggcgcttt tggtgcgact cgtcgatggc caggcggatg
      301 aactggccca gcccgctgtc gtccgccgcg ccgtgcacgt ggatcatggg cggcagggtg
      361 ctcaagagcg gattgaacgg gcgcgcgtcg cagccgagga atccgcacac caactggatc
      421 ttgtcgccgc cctcgccgcc ctcccggtag aagaacggca actgctcgtc ggccggccgc
      481 cggtgcgcct cccagtccgg ctcggcacgc attccggggg tgctcgacac cacgtgcgcg
      541 tcgccgtgcg ggaagacgat cacgtcgccg gcctcgagcc gtgccgcctc gccgcccagc
      601 agcccggccc agcaggcgcc gcgggtgacg acgtggtact cgatcacgtg ctccgagccg
      661 ggcatcacgg cggcggccac cgccttggcg ggcggcgctt gcgccaccca gggggcggcg
      721 gcctcgacga agaagtacac cgcgccttgc aggcggatcg cgcgcagcac gtcagacaaa
      781 gtatccgaac tcatccgcca cctcctggac agtcgagcaa gccgtctgga ctattgagca
      841 aatatctgag caaaagccgc tttttaagca agccgggcgg acggacaagc attcaccgcc
      901 gcgcctcccc ttcataaact tagcctcaca ccgacgaaaa ggccagttgc agcgccggtg
      961 cccatcaacc atcgacaagg agctgatcat gaccaccacc gtattgcaat gcttacctac
     1021 cgtcctcgat ttcgccgcga tcaaggcgcg gcagcaggcc acttgggcat cgggagatta
     1081 ttccatcatc ggcgcgacgc tgcagatcgt tgccgagtcc ctctgcgagg cgatcgacct
     1141 gcgcgccggc gagcgggtgc tcgatgtcgc tgctggcccc ggcaatgcgg cgatcgccgc
     1201 ggcgcgccgc tggtgcgagg tcgtcgccac cgattacgtg cccgccctgc tcgagcgcgg
     1261 gtacgaacgc gcggcggccg agggcctcac cgtggatttc cgggagggcg acgcggaatc
     1321 tctgccgctg cccgatgcgt cgttcgacgt ggtcctttct accttcggcg gaatgttcgc
     1381 gccggaccat tcacgggttg cccgcgagct gttacgcgtc tgtcggccag gcggacgcat
     1441 cggcttggcg aactggacgc cggaggggtt tatcggccag gtgttcaaaa ccgtcggcaa
     1501 gtacgtgccg ccaccggctg gcctaaagtc tccggcgctt tggggcaccg aagactggtt
     1561 gcgcgagatt ttcggggacc agatcactac gctgaaagcg acacgcaagc acttcgtttt
     1621 tcgctatcgc tcggcacagc attggttgga gacgttccgt acctactatg gccccatact
     1681 gaaggccttc gccgcgctgg ctgccgacaa gcaagacgca ctcgcagcgg atctcatcgc
     1741 tctcgcgcaa cggtccagca acagcgccga tacgttcgcc gcgccgagtg agtacctgga
     1801 ggtcgtgatc accaagcgct agacgacagg gatggagcca ctggtcgcgc ttgttcttcc
     1861 gccaagcgca aactttctta gccaatcgtt ttggagcaag ccatgcatac atcgatctac
     1921 caggagccca gacaaagcac ggcctcattc ctgaccacgg gcggagcgaa atgggcaaca
     1981 gggattatcg cgggttctct tgcggccgca gtgggcttca tgctcggcat cactgcggct
     2041 gcaagcacaa cgacgagcaa agagccgagc acgcctgata ccatccccgc gttcgtctac
     2101 tttccgccgc aatacgtcaa cgcagcgacc gaaattgacg agctgcctcc gcaattctaa
     2161 ccaaaggaga gaacaccatg tgcaagccat ccaaagtcac gatctttctg ctcgtccttc
     2221 acctcggcgg ctgcgcggcc ccggagttat cggagccgtt ggccgacgtg ccggacaagc
     2281 tcaaacccag cgcgaacgaa tcgcttgcaa tgatcgtccc cgcaaagggc gtgcaaatct
     2341 acgaatgccg cgccaggaag gaccagcctg gggcgtacga atgggcgttc gtggctcccg
     2401 aagccgacct cttcgacgtg cgtggaaata gaataggtcg acactatgcc ggcccgcatt
     2461 gggaatcaaa cgatggcagt aagatcgtgg gcacggtaaa ggaacgcgcc gacgcatcgg
     2521 tggccgacgc aatcccttgg ctgcttcttg ccgcgaagtc cgtcggctcg gaaggctcgt
     2581 tcagcaaggt tacgagcata cagcgcgtga atacggtggg cggcgtggcg cccaaggccg
     2641 gttgttctca agccgcggcc ggcacaccgg cacgcatcac ttacacggcg gactattact
     2701 tcttcaccgc caagtaagtt cgccttcgct cgctctgccg ccgagacacg gacgagatgc
     2761 cgcgccatcg tcatggctac tttgacaaag caacagcacg aagagacaag gagattcgtt
     2821 gagcgccttt tcccgggtcc cgcggccgat gctcggacgc aggcgcctca aagcatgctg
     2881 tcgtccctct cagcgcgacg ggcttcgcgc ttcaatggcg ggcagcgtct ggtgttatga
     2941 gaaccactcg agggaaatac gcgctgtagc gacgcgcatc gcgagtgagg atgggacagc
     3001 cactcaccgc cgcgtgagcc ccaatgaaga agtcggcgag tccgttgctt ttttttccgc
     3061 cctcgcgccg gtaccgtacg aaagctttgc cggccagaaa cagcgccggt ttgggcagct
     3121 cgcgcaggac aaggcccatc ttttcaactg ccgcatccag cgcctccact gtcttgaagg
     3181 cgagcgacag ctcggcgtag atcacggggt tgatgatcaa gtcatgaacc tgggcgagtg
     3241 cacgcagttg cttgagcgac cactcggccc aatcggcgtc atcctctaga acgtcgatca
     3301 ggacgttggt atccaccagc agcatcactc gtcgcgtagc agggccatca attcatcggt
     3361 gcgccacttg acttgcgctt tgcctcgcac tgccgcgaag cggtccggct tgctgctctt
     3421 gcgcgctccc accttgcgca gcaccacgtc gccatcgccg ttcactgcga actcgacgct
     3481 ggcgcccgga acaagcttca gcgcatcgcg gattttcttc gggatcgtga cttggccctt
     3541 gctggtaagg gttgtgggca tagcatctcc gcataagtat tactacaaga acgattgtaa
     3601 tacttaggaa gcagaagaac aaccgaccga gtcaaccaca ccatttcggg accagtagga
     3661 tatcccggct accgctgaaa ataggggaag gcagccacct ggacgaagac tgtcagcaag
     3721 atttccctgg cgatttattc gaaggcgtga acaatgttat tccgaaactt cgattattaa
     3781 aaccgagcga atgatctacg acgtggtcaa ctgacagaca ctttattttt atacaaagga
     3841 gaagtaacca tgagtgtgcg acgtttcacg agcaacgatg cgaagtttta ccagtacctg
     3901 gaccgcgaga tattcgtcgg cgatgttctg gattcatcca acagcgacac gatgagcgtc
     3961 ggcttctacc ggaacaagaa gaaaggcgag aaaaacgaat ggatcgtcac atatgacgag
     4021 gctctcatcg ttacgaaagg cgcgctcacc attcgtttcg atgagggcgc gaaaaccgcc
     4081 aaagtgggcg aggttatctt cctgactaag ggcacaaaga tcgcctacga ggccggtgag
     4141 gacgacacgg aggtcgtcta tgtgagctat ccgcattgga tggacgcgca aaagaaatcg
     4201 aagcacgccc acctgctcga ttcgtatcac ccggtgtgaa catggctagc agtgctttac
     4261 aacctccaat ttcgcgtcag cgaacaacga tgaacgcaag atctgacgaa gccgctgtcg
     4321 tggggttcga cctcgacgcc tacctggaga gaatcggttg cttgggcgag cgggcgccga
     4381 ccctggcgac gctcgccgtc attcagcttc ggcataccga agccatcgcg tttgaaaact
     4441 tgaacccgct gatgggctgg ccggttcgcc tcgatatgga atcgctgcag gagaaaatag
     4501 tgcgcgacgg acgcggcggc tattgctacg agcacaatct gcttttcaaa catgcgctcg
     4561 aagcgctcgg ctttggcgtc aagggtcttc tggcgcgcgt tctctggagc gcaccgaaag
     4621 gtgcgatcac acctcgcagc cacatggtgt tgcaagttga tctcgacggc gagccataca
     4681 ttgtcgatgt cggattcggc ggcgttacct tgaccggacc tcttcgactc atcgctgata
     4741 tcgagcaggc gacgccacat gaacccttcc gcttgcgcag agtggacgac gaatttatcg
     4801 aagaggtaaa aatccgcgac gcttggatac cgctctaccg cttcagtttg caggaagctt
     4861 ttttacccga ttacgaaatc aacaactggt acacctcgac tcatcccgac aacctcttcg
     4921 tgaacgagct actcgccgcg cggccagcac ctaatcgtcg ctatgcgctg cgcaacaatc
     4981 aattcaccgt gcaccatttg accggaagaa ctgaccggcg catcctcgcg aacgcggatg
     5041 agatgcgcga gattctcgaa ggtccgttcg gtcttacgtt gcctgatgcg cgggagcttg
     5101 atgctactct caagcgcttg acagcgtcgt cgtgacgctt gcggtgagaa gcggcggttc
     5161 ttagaaggtg agattggtct aaggaatctg gtagcctgca atcctttacg cacgagtctt
     5221 gcacgttcgg cgcaaacaag gaggcaagca tgaagcagtt acaaaaagca cagatcgacg
     5281 agttcaaagc acacttcggg ggggaagtgc ttctttctgg cgaagcgggc tacgacgagg
     5341 tgcgccagat ctggaacgcc atgatcgacc gcaggcccgc gctgatcgtc cgctgtgcgt
     5401 ctcctgaaga tgtcgtccag gccgtcaagt tcgcgcgcaa gcacaacctg ctcgtctcaa
     5461 tccgcggggg cgggcacaac atcgcgggca atgccgtctg cgacgatggc ctcatgatcg
     5521 acctctcgct gatgaagagc gtcgaggtag acccgaacgc ccggcgggcg atggtcgagc
     5581 ccggctgcac cctcgccgac ttcgacgcgg ccacgcaggc gcacggcctc gccacgccgc
     5641 tgggcatcaa ctcgaccact ggcgtggccg gcctcacgct cggcggcggc tttggctggc
     5701 tgagcaggaa gtacggcatg accatcgaca acctgctctc cgcggatgtc gtcaccgcgg
     5761 atggcaatca ggtgcacgcc agcgaaacgg aaaacgctga cctcttctgg gggctgcgtg
     5821 gcggcggtgg gaacttcggt attgtcacca gcttcgaatt ccatctccat cccgtcgggc
     5881 cggacgtgct gacggggctc atcgccttcc cgttcgccca ggctaagtcc gtcctcaagc
     5941 agttcgcccg cttcaccgag acgatgccgg acgaacttaa tgtctggatg gtcactcgga
     6001 aagcgccccc actgccgttc ctgcctgagg acgtccacgg gaaagagatc gtcgtgctcc
     6061 ccctgtgcta cgcgggcgac ccccgtgagg gcgagaagct gatcgagccg ctacgcgggt
     6121 tcggtacggc gcacggcgag cacatcggcg tgcagccgta caccgcctgg cagcaggcct
     6181 ttgatccgct actgacacga ggtgcgcgca actattggaa gtcgcataac ttctcgcagc
     6241 tcagcgacgg cgcgatcgac accatcatcg aatacgccgg cacgctgccg tcgccgcaat
     6301 gcgagatctt cgtcggcacg atcggcggcc agacggcccg cgtagcgccc gaggcgatgg
     6361 cctactcgag ccgagacgcc aactacgtgc tcaacgtgca cgctcgctgg gagtcggccg
     6421 ccgaggacaa gcgctgcatc gcctgggcgc gcgaattctt cgccaagtca cagccgttcg
     6481 cgagcggtgg cgcgtacatc aacttcctca cccaagacg
//
LOCUS       DBNGSB010000084        11769 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_671039, whole genome shotgun sequence.
ACCESSION   DBNGSB010000084 DBNGSB010000000
VERSION     DBNGSB010000084.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 11769)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11769)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11769
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_671039"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..1313
                     /gene="ftsW"
                     /locus_tag="AC159B_02350"
     CDS             186..1313
                     /gene="ftsW"
                     /locus_tag="AC159B_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HIR3376628.1"
                     /translation="MMDLVKKRAPDFWLLLSTIALTGVGVVMVYSASSIVAHDRFGDS
                     AFFFKRQVLWVLLGTAAAMIAQRIHYERLSSLTPLIVLGALIMLVLVLVPGIGRVAGG
                     ARRWLSIGPASFQPAEAAKLAMVMYMARFLTIRAAGTQAFRRIVLPPVLLAGIMFALI
                     LLQPDMGSAVLVGVIMMIMLFVGGARLTHLFGLGAASAPFLAGAVLGEEYRRRRILAF
                     LDPWADPQGAGFHIIQSLLALGSGGLFGVGLGGSRQKYFYLPERHTDFIFSILGEELG
                     LIGTVGVLLLFGVVASRGFRIARSAPTRYAGLLATGLTAMIVSQAVINIGVAIGMLPI
                     TGVPLPFLSFGGSSLILTMIAVGILLNISQYTKIGKGGHGS"
     gene            1389..2477
                     /gene="murG"
                     /locus_tag="AC159B_02355"
     CDS             1389..2477
                     /gene="murG"
                     /locus_tag="AC159B_02355"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HIR3376629.1"
                     /translation="MRILITGGGTGGHVYPGLAVAEALRRLEPGVDFLFVGGRGIEQR
                     IVPQTGMRFAPVLSRSWPRRPTLRWLPAVALLGAGTAQAVGLLLRFRPDVVLATGGYV
                     SIPAGVAGALLRVPLVVQEQNAIPGATNRLLGRWARAVSIPHESVAPFFRGKSIVTGV
                     PVREHALRGDRSRGLRRFGLRDGVLTVLVLGGSLGARALNETVVEMARLLPKESGVQI
                     LHQTGPDHDEWVREQIRPPGPMPTYVVMPYIEDVGDAYACADIVISRAGAGTLAEVTA
                     NGLPAIAIPYPFAVAGEQEANARMLEAAGAAVVILHRDLNGSRLRDTLVELRDPSRRS
                     AMADASRRLGRPLAAHAVGEIVLRLARA"
     gene            2487..3914
                     /gene="murC"
                     /locus_tag="AC159B_02360"
     CDS             2487..3914
                     /gene="murC"
                     /locus_tag="AC159B_02360"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIR3376630.1"
                     /translation="MIGKDARVHFIGIGGAGMSAVAFVLLAKGYRVSGSDLRRTPITE
                     RLQAKGATIVVGHDTRHVAEADVVVASRAVPDLNVEIVAAHAKDIPVLHRAQVLGQIL
                     AEGFGIAVVGTHGKTTTSAMAAHVLTAGGLDPTALIGADVETLGGNVRVGSGKYVVAE
                     VDESDGSHVYVRPAAAVVTSLDITDHRDYYKTLPQLMTTFAGFLALISPEGYVVMCTD
                     ARHVRELVPAVRAHVISYGLSNGSDYTATIHAMEGRRTVCTFRRQGRALGKVQLNVPG
                     RYNVKNALAAAAVGIEAGLAFTAIAGALETFGGVSRRFSVRGEVDGIMVVDDYAHNPV
                     KVSSVLRAARESWPKRRLIAIFQPHRFSRTKTTFRQYSLAFKDATDLIITDIYPADEQ
                     PIPGVTADLIVDVVRPTRQVQYVRDKHELVQTLLPTLRTGDLVITLGAGDIWRIADDL
                     VAALQKRSPAPALPSSQSSPSPPSN"
     gene            3986..4912
                     /gene="murB"
                     /locus_tag="AC159B_02365"
     CDS             3986..4912
                     /gene="murB"
                     /locus_tag="AC159B_02365"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIR3376631.1"
                     /translation="MNPVSAEIIADLRKLCREVRENEPLKRHVSFRIGGPADVLVLPR
                     SSDELRKVAGWLFTHNVPFEILGHGSNVLIADGGIRGIVIKIGKGMDRFSFKGTRLVA
                     EAGAGLPHLARTAATKGFSGLEFAAGIPASLGGAVVMNAGAHGHAMSEVLREVRVLVP
                     EGEQVLGKSDLGFAYRTTVLQRRRAVLLEATIELTQSDPVTVRARTDEWLKHRSDTQP
                     IGPPSSGCVFRNPPGDHAGRLIDVAGAKGLAVGDARVSEVHANYIVNGGEAKAADVVG
                     LMHKVSGLVAEKFGVRLDPEIKLLGEFAQTTH"
     gene            4937..5740
                     /locus_tag="AC159B_02370"
     CDS             4937..5740
                     /locus_tag="AC159B_02370"
                     /inference="COORDINATES: protein motif:HMM:NF015739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIR3376632.1"
                     /translation="MRTTTAGSLPASPSSPEAASLALLFESFLRFFTIMTVLLAAAAL
                     PLSSLFSVTEIVVEGASQISAHKIATRSGVRIGDPRFGVQAVEIAARVARHPKIAKAT
                     VVVQPSGSVQIEVTERQAAAAIPYHNVFLLVDALGVLLEKRVDRAGLPLLRIEDLSLP
                     WVRVGDEVPTPQIGQALRVLQLLPATAKRGPLEIRMDSADEFSIVTAGGLRVILGPLR
                     GLGERASLLPQVMSELRERNISAQYVDLRVLDNVVLRPVAPRGGEGDAP"
     gene            5816..7045
                     /gene="ftsA"
                     /locus_tag="AC159B_02375"
     CDS             5816..7045
                     /gene="ftsA"
                     /locus_tag="AC159B_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009109895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HIR3376633.1"
                     /translation="MRKEHITGLDIGTTKVCAVVGEVDGGEVHVAGVGSVPSSGIRKG
                     VVIDIEATTNAIEDAVERAERMSGEEIRGLYVGISGEHISSTNSRGIIAISRGDHEIS
                     PSDVERVIDAARMAALPASDREIVHLLPRGFVVDGHDGVRNPVGMYGARLEVEAHIVT
                     GTSTVLANLLKCVQRAGLEVEEIALEPLASAQAVLTSAERELGVVLADIGGGTTSLGV
                     FVAGGLCHTAILPVGGHHLTADVAVGLRTPVAEAEKLKIRYGAASVQDASEGEMIEVF
                     NVGDREPRVLPRRVLCEIIEPRLQEICSLMRLQIRRSGFAHVVPAGIVLTGGTALLRG
                     IAKFVSEKLELPARVGMPDHLGGLVDAVRSPIFSTGVGLVLYGVRHGGAKAVRTTNGH
                     GLVGRMRGWIREMVHGA"
     gene            7191..8249
                     /gene="ftsZ"
                     /locus_tag="AC159B_02380"
     CDS             7191..8249
                     /gene="ftsZ"
                     /locus_tag="AC159B_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIR3376634.1"
                     /translation="MAHIDRDLRRYAAIKVVGVGGGGSNAVNRMISAGLRGVEFIAIN
                     TDAQALAMSNADKKIHIGGKITKGLGAGGDPEVGRQAAEESKDDLFEAVEGADMVFVT
                     AGMGGGTGTGGAPVIAQIGRELGSLTIGVVTKPFSFEGRRRATSADEGVRNLKQKVNT
                     LITIPNDRLLQLIDRASTLIDSFRVADDVLRQGVQGIADLITVPGLINLDFADVRTVM
                     HEAGSALIGIGVATGEDRATRAAQAAISSPLLETSMEGARGVLINVTGGLDLGLVEVS
                     EAAQIVKEAADPEANIIFGAVIDDKIDGEVRITVIATGFDSAKKRDDQPLETGDSIED
                     GVKALISDLDIPAFLRKR"
     gene            8376..9458
                     /locus_tag="AC159B_02385"
     CDS             8376..9458
                     /locus_tag="AC159B_02385"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR3376635.1"
                     /translation="MGIELTWRLEEFREVVNEYFPVMDLWFDDGRPAFVVQVTPDSRQ
                     RFLQLRQRLEPLGYLPLLRRRDGREIVALLPRPAPGQWRWGVNLALFLATLVTTFYAG
                     YLNAISLVADGFLRSAVGGGLAFALSLMLILATHEMGHKVFSIIRGVDASLPYFIPVP
                     PVAGLPTIGTIGAVIVTRTPAPNRDALMDVGAAGPIAGFLVAIPILVIGIARSFVISP
                     VGFEGINLPDPLLVKLLIGIILRPTQDAVVLGHPVLFAGWIGLLVTSINLLPAGMLDG
                     GHAVRAIFGRRHHRWLSWVAVGIAVVLGYWLMAVLIVAMARRGHPGPLDDVSPVSSSR
                     IITGVALLLIFILSAVPLFPLGDFWR"
     gene            9464..10654
                     /locus_tag="AC159B_02390"
     CDS             9464..10654
                     /locus_tag="AC159B_02390"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3376636.1"
                     /translation="MLAVLRKELIEALRDRRTIIVALILPVLMMPVATLGIPYLAERQ
                     AAELEDRPALIAVVGAEHARSLVALGLRRDWIRVVDVKEDASRALRNREIEAVLIIPP
                     GFLETLAAGRASVTVSYDESDLASRVARERLARLIATYSIEVTERRLERRGLSRRDLA
                     PIDVIERDVASRTRLGGLLLAGLLPFFIAVWAVLGGQYAALDVGAGERERRTLEALLV
                     TPPPRWALAAGKFLAISMASFAAVLVVIVVTMLTLRLGAALRIAELERASITISPTAS
                     GLLLLVALALVGFLSAVELALSLAARGVREAQQYFTPVYLMFTLPAMAAQFLEGWTRS
                     WWTYLIPGLNAVFALRALLLGEWRPEHLTLAVISTALYTLPMLWISAWILGREPSASG
                     SRQS"
     gene            10817..11341
                     /gene="nrdR"
                     /locus_tag="AC159B_02395"
     CDS             10817..11341
                     /gene="nrdR"
                     /locus_tag="AC159B_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012574219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HIR3376637.1"
                     /translation="MKCPYCSHEESKVLDSRPVDDGASIRRRRECEKCSRRFTTSERM
                     DHAPVMVVKRDGRREPFLRDKILQGLVRAAGKRPVSMDELERIVTEVEREVLNRGEHE
                     INTQQIGALVMDHLRRLDDVAYVRFASEHRRFRDLDTLVEEAGIVKERRRQEEALRAQ
                     VPLITVDANTKTTK"
     gene            11407..>11769
                     /locus_tag="AC159B_02400"
     CDS             11407..>11769
                     /locus_tag="AC159B_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012537.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HIR3376638.1"
                     /translation="MDADAVATTPTTSDLPQETLEFFTGDDLRARVFHDKYALRDLAG
                     RVVESTPPQMWSRIARELASVEVTPAKREEWEQKFYWLLEDFRFIPGGRIMHGAGNPK
                     RVTNLNCYVVPVRDDSIES"
BASE COUNT         2085 a         3598 c         3803 g         2283 t
ORIGIN      
        1 cgggtgaggc agtgctgtta tcaccaggct gtgaaagctt cgaccagttc accgattacc
       61 gccagcgtgg cgacaggttt gtgaacctcg tccaggccct gtcagtcaaa gcgtaaccca
      121 agaggacttc cgcagccggc aggcaacatc tagtagggca attcgacgga ccttcgtcgc
      181 agcacatgat ggacctggtc aagaaacgcg cgcccgactt ctggttgttg ctctcaacca
      241 tcgccctcac gggagtgggt gtggtgatgg tgtacagcgc gagctcgatc gtggcgcacg
      301 accgcttcgg agacagcgcg ttcttcttca aacggcaggt gctctgggtg ctgctgggca
      361 cggccgccgc gatgatcgca cagcgcatcc actatgagcg gctcagttcc ctcacgccgc
      421 tgatcgtcct cggtgccctt atcatgctcg ttctcgtgct cgtgcccggc atcggacgcg
      481 tggccggggg agcgcgccgg tggctcagca tcggaccggc gtcgtttcaa ccggcggagg
      541 cggcgaaact tgcgatggtg atgtacatgg cgcggttctt gaccatccga gccgccggca
      601 cgcaggcgtt caggcgcatc gtgctgcccc ccgttttgct cgccggaatc atgtttgcgc
      661 tcatcctgct ccagccggac atgggttctg ctgttctcgt cggcgtcatc atgatgatca
      721 tgctgttcgt cggcggcgca cggctcactc acctcttcgg tctaggcgct gcgagcgccc
      781 cgtttctcgc cggcgccgta ctcggagaag agtaccgccg gcggcgcatc ctggcattcc
      841 tggatccttg ggccgacccg cagggcgcgg gtttccatat tattcagtcc ctgcttgcac
      901 tcggctccgg cggtctcttt ggggtgggcc tcggggggag ccgccagaaa tacttctatc
      961 tgcccgagcg ccatactgat ttcatctttt cgattctcgg tgaagagctc ggcttgatcg
     1021 gaaccgtggg agtgctgctg ctcttcggtg tggttgcgtc ccgtgggttt cgcatcgccc
     1081 gctcggcgcc cacgcgatat gcggggctgc tggcgacggg tctcacggcc atgatcgtgt
     1141 cgcaggccgt catcaacatc ggcgtggcca tcggcatgct tccgatcacc ggggtaccgc
     1201 tgccgttttt gagcttcggc ggatcctcac tgattcttac gatgatcgca gtcggaattt
     1261 tgctgaacat ttcccagtac accaaaatcg gcaaaggcgg tcatgggtcg taggtcgctg
     1321 gtcgtggcca cggcatttcc tacgacctac gaccgacgac ctgcgaccgc agtttccctt
     1381 tagtcgtcat gcgcattctc atcaccggtg gcggcacggg cggccatgtc tatccgggac
     1441 ttgcggtcgc tgaagcgctg cgccgcctcg agccgggcgt agatttcctc tttgtcggtg
     1501 gacgcgggat cgaacaacgg atcgttcccc agacggggat gcgattcgcg cctgtgctat
     1561 cgcgttcgtg gccgcgccgg cccacacttc gctggctgcc ggcagtcgct ctgctcggcg
     1621 ccggaacggc gcaggccgtc ggactgctgc tgcggttccg gcctgatgtg gtcctcgcga
     1681 cgggcggcta cgtgtccatt cccgccggcg ttgccggagc gctccttcgc gttccgcttg
     1741 tcgtacagga gcagaatgcg attcccggag cgacaaacag gctgcttgga cgatgggcgc
     1801 gggccgtctc gattccgcat gaaagcgttg ccccgttctt ccggggcaaa tcgattgtaa
     1861 ccggcgttcc tgttcgggaa catgcgcttc gtggggatcg gagccgcggg cttcggcgct
     1921 tcggtctcag ggatggggtg ctcacggtgc tcgtgctggg cggaagcctg ggggcgcggg
     1981 cgctgaacga aaccgtggtc gaaatggcac gtctgctgcc taaggaatcg ggggtacaaa
     2041 tactgcatca aaccggacca gaccatgacg agtgggtgcg cgagcaaatc cgcccgcccg
     2101 gccccatgcc cacctacgtc gtgatgccat atattgagga tgtcggtgat gcgtatgcct
     2161 gcgcggatat cgtcatcagc cgcgccggtg ccggcacgct tgctgaagtc acggcgaacg
     2221 gattaccggc gattgccatc ccgtatccgt ttgcggttgc cggcgagcag gaggccaatg
     2281 ccagaatgct ggaagccgcg ggggcggctg tggtgatcct gcaccgggac ttaaatggaa
     2341 gccggctccg cgacacactg gtggaacttc gcgatccatc gcgacgtagt gcgatggcgg
     2401 acgcgagtcg acggctcggc cgacccttag cggcccatgc cgtgggtgag atagtgcttc
     2461 gactagcgag ggcttgaggg tacaccgtga tcgggaagga tgcgcgggtc cattttatag
     2521 gcatcggcgg cgctggaatg agcgccgtcg cgtttgtact gttggcgaag gggtatcggg
     2581 tcagtggctc agacctacgt cgcacaccga tcaccgaacg cctccaggcg aagggcgcaa
     2641 ccatcgtcgt cggtcatgac acccgtcacg tcgcggaagc cgacgtggtg gtggcctcac
     2701 gcgccgttcc cgatctgaat gtcgagatcg tggccgcgca cgcgaaggac atccccgtcc
     2761 tgcaccgggc gcaggtgctg ggccagattc tggcggaggg gttcgggatc gcggtcgtcg
     2821 gcacgcacgg gaaaacgacg acgtctgcga tggcggcgca cgtgctgact gccggaggcc
     2881 tcgatccgac ggcgctgatt ggagccgatg tcgagaccct cggcggaaac gtgcgggtcg
     2941 gcagcggcaa atacgtggtg gcggaggtcg atgaatcgga tggatcccac gtgtacgtcc
     3001 ggccggctgc cgccgtagtc accagcctgg acatcaccga ccaccgggat tattacaaga
     3061 cgctgccgca gctcatgacc acgtttgccg gtttccttgc cctgatctca ccagaaggct
     3121 acgtggtcat gtgcaccgac gcccgacacg tgcgcgagct cgtgccggcg gtccgggcgc
     3181 acgtcatctc gtacggtctg agcaatggaa gcgactatac ggcgaccatc cacgcgatgg
     3241 aaggacgccg taccgtctgc acgttccgca ggcagggacg cgccctcggc aaggtgcagc
     3301 tcaatgtccc gggccgttac aacgtgaaaa acgccctcgc ggccgcggcc gtaggcattg
     3361 aggccgggct ggcattcacg gccatcgccg gcgcactcga gacgtttggg ggagtgagcc
     3421 ggcggttctc ggtgcgcggc gaggtggacg gcatcatggt ggtcgatgat tacgcccaca
     3481 accccgtgaa agtgtcgtct gtgcttcgtg ctgcgcggga gtcctggccc aagcgacgcc
     3541 tgatcgcgat ctttcagccg caccggttct cgcggaccaa aaccacattc cggcagtact
     3601 cactggcatt caaagatgcc accgatctca tcatcacgga catctatccc gccgacgaac
     3661 agccgatccc cggggtaacc gcggacctca tcgtcgacgt cgtgcgcccc acccgtcaag
     3721 ttcaatacgt gagagacaag catgagctgg tgcagacgct cctgccgacc ctccgcactg
     3781 gagatctggt gatcacgctg ggggcggggg acatctggcg aatcgcggac gacctggttg
     3841 ccgctctgca gaagcgcagt cccgctcccg cgctcccgtc ttcacagtct tccccgtctc
     3901 ccccgtctaa ctgagaggtg cttgctacat tgcagttcag catgagactg ggaagacagg
     3961 gaagacggag tagactgggt agacgatgaa cccagtgtct gcagaaatta ttgccgacct
     4021 ccgaaagttg tgccgcgagg tccgagagaa tgaacccctc aaacggcatg tgtcctttcg
     4081 aatcggtggg cctgccgacg tgctggtcct tccgcgatcc tcggacgagc tccgcaaggt
     4141 tgccggctgg ctgttcacac acaacgtccc gttcgagatc ctcgggcatg gtagcaatgt
     4201 cctgatcgcc gacggcggca ttcgaggcat cgtcatcaag atcggcaaag ggatggaccg
     4261 gttttcgttc aagggcacgc gtcttgtggc tgaggccggc gccgggcttc cacacctcgc
     4321 gcgcaccgcg gccaccaaag gattctctgg gctggagttt gctgcgggga ttcccgcgtc
     4381 gctcggtggc gctgttgtga tgaatgcagg cgctcacgga catgcaatgt cagaagttct
     4441 ccgcgaagtc cgcgtgctgg tccctgaagg agagcaggtg ctgggaaagt cggacttagg
     4501 tttcgcatac cggacgaccg tgcttcaacg gcgccgggcc gtgctgctcg aagccacaat
     4561 agagctcacc caaagtgatc ctgtgacggt tcgagcgcgc accgacgagt ggctcaagca
     4621 ccgcagcgac acacaaccga tcggcccgcc gagttccggc tgcgtattcc gtaatccgcc
     4681 cggggatcac gccggacgcc ttatcgacgt cgcaggagcc aaaggattgg cagtcggcga
     4741 tgcgcgcgtc tccgaggttc acgccaacta cattgtcaac gggggagaag caaaggcggc
     4801 cgacgtggtc ggcctgatgc acaaggtatc aggactggtc gcagagaagt tcggagtgag
     4861 gctggatccg gaaatcaagc tcctgggaga gttcgcacag acaacgcact agccgtcgcc
     4921 ctttcgtcgt actttgatgc gcacaaccac cgccggctcg ctcccggcct cgccgtcctc
     4981 ccctgaggct gcgtctctcg ccctcctctt tgagtcgttc ctgcgtttct ttaccatcat
     5041 gaccgtgctc ctggccgcag cggccttacc tctatcgtca ctcttttccg tgactgaaat
     5101 cgtcgtggag ggggccagcc agatctccgc gcacaagatt gccacccgat ccggcgtacg
     5161 tatcggtgat ccacgatttg gcgtccaggc agtcgaaatc gccgcgcgtg tagcgcgtca
     5221 cccaaagatc gccaaagcga ccgtcgttgt gcagccctct gggagcgtcc agattgaagt
     5281 gacggaacgc caggcggcgg cggcgattcc ctatcacaac gtattcttgc ttgtagacgc
     5341 gttgggagtg cttttggaga aacgcgtgga tcgggcgggc ctgccgctgc tgcggatcga
     5401 agatctctcg ctgccctggg tgagagtagg agacgaagtc cctacgccac agatcggcca
     5461 ggcactccgt gtgcttcagc tgcttcccgc gacagccaaa cgcggccccc tagaaatcag
     5521 gatggactct gctgatgagt tttcgatcgt caccgctggt gggctacggg taatcctggg
     5581 cccccttcgg gggcttggtg agcgcgcgtc gctgcttccc caggtgatgt cggaattacg
     5641 tgagcggaac atctcagccc agtacgtgga cctgcgcgtc ctcgacaacg tcgtgctcag
     5701 accggtcgct ccacggggtg gagaaggaga cgcaccctga ctggtggaat cctggcccaa
     5761 gatatagggg ataacttgcg catagctaca acatgtagaa ggtgcaggaa acgagatgcg
     5821 taaggagcac atcaccgggc tggatatcgg gacgacgaag gtctgcgccg tcgtcggcga
     5881 ggtggacggc ggcgaggtgc acgtcgccgg cgtcggcagc gtcccgtcct caggcattcg
     5941 caagggcgtc gtcatcgaca tcgaagcgac gacaaatgcc attgaagacg ctgtggagcg
     6001 cgcggagcgc atgtctggag aagagatccg cggtctctac gtgggaattt cgggagagca
     6061 catcagttct acgaactcgc gcgggataat cgcgatcagt cgcggcgacc acgagatcag
     6121 tccttcggac gtcgagcgcg taatcgacgc ggcccggatg gcggcgctgc cggccagcga
     6181 ccgagagatc gtccacctgt tgccgcgcgg gtttgttgtc gacggccacg acggcgtgcg
     6241 gaatccagtg ggcatgtatg gcgcgcgcct cgaggtcgag gcacacatcg tgaccggaac
     6301 atcaacggtc ctcgccaacc ttctcaagtg cgtacagcgg gccggactcg aagtcgagga
     6361 gatcgcgctc gagccgctcg catccgcaca ggcggtgctg acatcggcgg agcgagaact
     6421 gggggttgtg ctggccgaca tcggcggcgg cacaacgagt ctcggcgtct ttgttgccgg
     6481 tggcctgtgc cacacggcga tcctgcctgt gggaggccac cacctgaccg ctgatgtggc
     6541 cgtgggactg cgcacgcctg tcgcagaggc ggagaaactg aagatccggt acggcgctgc
     6601 gtcggtgcag gacgcgagcg agggcgagat gatcgaggtc ttcaacgtgg gcgaccggga
     6661 gccgcgcgtt ctgccgcggc gcgtgctctg cgagatcatc gagcctagat tgcaggagat
     6721 ctgcagcctg atgcgtttgc agatccgtcg cagcggtttt gcacacgtcg tgcccgcggg
     6781 catcgtgttg accggcggga ccgcgctgct gcggggaatc gccaaattcg tcagtgaaaa
     6841 gctcgagctg cccgcccgcg tgggcatgcc tgaccacctg ggcggccttg tggatgcagt
     6901 gcgcagcccg atcttctcga caggagtggg acttgtccta tacggcgtcc ggcacggggg
     6961 cgcaaaggcg gtgcgtacaa ccaacggtca tgggctggtg gggcggatga ggggatggat
     7021 acgggagatg gtgcacggcg cctaagtaaa gctgaagagg tggaaagctg gaaagctgaa
     7081 gagcagacgc ccgatctccc tttccagctt ttcagctctc cagctctcca gcttcagtta
     7141 cgacgtagcg acaacgtagc atgggccccg gggcccggag gggagtagga atggcgcata
     7201 tcgatcgcga tctacggaga tacgcggcga tcaaagtcgt gggagttgga ggcggcggca
     7261 gcaatgccgt aaaccggatg atcagcgccg ggttgcgcgg cgttgaattc atcgccatca
     7321 acaccgacgc gcaggcattg gcgatgagca acgccgacaa gaagatccac atcgggggca
     7381 agatcaccaa gggcctcggg gccggcggtg atcccgaagt gggccggcag gccgcggagg
     7441 aaagcaagga cgacctgttc gaggcagtcg agggcgcgga catggtcttc gtcaccgccg
     7501 gcatgggagg cgggacgggt accggcggcg cgcctgtcat tgcgcaaatc ggccgcgagt
     7561 tgggttccct caccatcggg gtggtcacca agcccttctc cttcgaaggg cggaggcgcg
     7621 cgacgtcggc agacgaaggc gtacgcaatc tcaagcaaaa ggtcaacacg ctgatcacca
     7681 ttccaaatga ccggttgctg caactcattg accgcgcgtc cacgttgatc gattcgttcc
     7741 gcgtcgcaga cgatgtgttg cgacagggcg tgcagggcat cgcggacctc atcaccgtgc
     7801 cggggctcat taacctcgac tttgccgacg tgcgaacggt catgcacgag gcggggtcgg
     7861 cgctcatcgg catcggggtg gcaacgggcg aggaccgcgc cacgcgagcg gcacaggccg
     7921 cgatcagcag ccccttgctc gagacctcaa tggagggtgc gcgcggtgtg ctcatcaacg
     7981 tcacgggcgg cctggacctg ggactggtgg aggtcagcga agcggcgcag attgtcaaag
     8041 aggccgccga tcccgaagcc aatatcatct tcggcgcggt gattgacgac aagatcgacg
     8101 gagaagtgcg cattaccgtc attgccacgg gattcgattc ggcaaagaaa cgcgacgatc
     8161 agccgcttga gaccggggac tcgatcgagg atggggtgaa ggcgctcatc agcgatctcg
     8221 acatacccgc attcctgcgg aagcgataag gaaaaaggaa taaggaaaac ggaaaaaggt
     8281 aaagacaagc caagggatgg agaggggact gacgctggtc ccttttccat tttccctctt
     8341 cccttttcct tccgccccag cgtacgctgg agggcgtggg catcgagctg acgtggcgcc
     8401 tggaagaatt tcgtgaggtc gtcaacgagt actttccggt gatggacctg tggtttgacg
     8461 acgggcggcc ggcgttcgtc gtgcaggtca ctccagactc aaggcagcgc tttctgcaac
     8521 tgcggcagcg gctggagccc cttggttatc tgccgctact gcgacgcagg gacggccgtg
     8581 agatcgtcgc actgctcccc cgcccggcgc ccggtcaatg gcgttggggg gtaaacctcg
     8641 cgctttttct ggcgacactg gtcacaacgt tctacgcggg ctacttgaac gcgatctcgc
     8701 tggtcgccga tgggtttctc cgcagcgccg tcggcggcgg gttggcattc gcgctgtcgc
     8761 tcatgctcat cctggctaca catgaaatgg gtcacaaagt cttctcgatt attcgcggtg
     8821 tcgatgcgag cctaccctac ttcataccag tcccgcctgt cgccggtctg ccgacaatag
     8881 gcaccatcgg cgcggtgatc gtaacccgca ctccggcgcc gaatcgagac gctcttatgg
     8941 atgtcggtgc agctgggccg attgccggat ttctcgtcgc catccccatc ctcgtcatcg
     9001 gcattgcccg gtcgtttgtg atcagcccgg tcggcttcga aggcatcaac ctccctgatc
     9061 cgctgctcgt gaaactcctg atcggcataa tccttcgccc gacgcaggac gccgtcgtgc
     9121 tggggcatcc tgtcttgttt gccggctgga tcggcttgct cgtgaccagc atcaacctgc
     9181 ttcccgcggg gatgctcgac ggcggccatg ccgtacgagc gattttcggc agacgccatc
     9241 accgctggct gtcctgggtc gccgtgggga tcgcagtcgt gcttggctac tggctcatgg
     9301 ccgttctcat cgtcgccatg gcgcggcgcg gtcatccagg tcccctcgac gacgtctctc
     9361 ccgtcagttc ctcgcgcatc atcaccggtg tggcgctgct gctgatcttc atcctcagcg
     9421 cagtcccgct gttcccgctt ggcgacttct ggcgatgacc tcgatgctcg cggtcctccg
     9481 caaggagctc atcgaggcgc tgcgcgaccg gcggacgatc atcgttgcgc tcattctgcc
     9541 ggtgctgatg atgccggtcg cgaccctggg tattccatat cttgccgaac ggcaggcggc
     9601 ggaacttgaa gaccgccctg cgttgatcgc cgtggtcggt gctgaacacg cacgaagtct
     9661 agttgcgctc gggctgcgcc gggactggat tcgcgtagtc gacgtcaaag aagatgcgtc
     9721 gcgggcgctt cggaaccgcg aaatcgaagc cgtcctgatc atccctccgg gcttcctgga
     9781 aacgctcgcc gccggcagag cgtcggtaac ggtcagctac gacgaaagtg atctggcgtc
     9841 ccgcgtggca cgagagcgcc tggcgcggct gattgcgacc tacagcatcg aggtcaccga
     9901 acgacggctg gaacggcggg ggttgtcacg ccgggacctt gcaccgatcg atgtcataga
     9961 gcgcgatgtg gcgagcagga cccgtctggg tggcctcctc ctcgcaggtc tgctgccgtt
    10021 cttcatcgcg gtctgggccg tacttggagg ccagtatgcc gcccttgatg ttggtgcagg
    10081 tgaaagggag cgtcgaactc tagaagcatt gctcgtcacg ccacctccca gatgggcatt
    10141 ggcggccggt aagtttctcg ccatctccat ggcaagcttc gcggcggtac ttgtagtgat
    10201 cgtggtgacc atgctgacgc tccgccttgg agccgccttg cggattgccg agctcgaacg
    10261 ggcttccatc acgatctccc cgaccgcctc cgggctgctc cttctcgtcg cgctggcgct
    10321 tgtgggcttc ctgagcgccg tcgagctggc gttgagcctc gcggcccggg gggtccggga
    10381 agcacagcaa tacttcaccc cggtctatct gatgttcacg ctgccggcca tggctgctca
    10441 gttcctggaa ggatggaccc gttcgtggtg gacatatctt atccccggcc tcaacgccgt
    10501 cttcgctctc cgtgccctgc tgctgggcga atggcgaccc gaacacctca ccctcgcggt
    10561 catctcgacg gccctgtata ccctccccat gctctggata agtgcctgga tcctgggccg
    10621 cgagccctcc gcctccggtt cccgccagtc ctgaagcggg tccgcacagg ccggcaccca
    10681 agcgcgtgac cgcttccctc cgaggctttc tctcctctgc agcgtggcaa atttggtgat
    10741 tgacacacaa gatatggagg actagaatcg gttttgccct aaatgtggta ggactgctga
    10801 gggcagcccc aagcgcatga agtgccccta ctgttcgcac gaagagtcaa aagtcctcga
    10861 ctcccgtcct gttgacgacg gcgcctcgat acggcggcgg cgggagtgcg aaaagtgcag
    10921 ccgccgcttc acaacctctg agcggatgga ccacgcgccc gtgatggtcg tcaagcgtga
    10981 cgggcggcgc gagccgttcc ttcgcgacaa gatcctccag ggactcgtcc gtgccgccgg
    11041 gaagcgacct gtttccatgg acgagctcga gcggatcgtc accgaagttg agcgcgaagt
    11101 cctcaatcgc ggcgagcacg agatcaatac ccagcagatc ggagccctcg tgatggatca
    11161 cctgcggcgt ctggacgacg tagcttacgt ccgttttgcc tctgagcatc gccggttccg
    11221 cgacctcgac actctggtgg aggaagccgg aatcgtcaaa gagcgccggc ggcaggaaga
    11281 ggctttgcgc gcgcaggttc cgctgattac tgtcgacgcg aacacgaaaa caaccaagta
    11341 acgagatttt tcatgcgccc cggatgtccg tgaccggggc gacattgcct caaaaggggt
    11401 gaacccgtgg atgccgacgc cgtagcgacc acaccaacga cctctgatct gccccaggag
    11461 acccttgagt tcttcaccgg cgacgatctg cgggcgcgtg ttttccacga taagtacgcg
    11521 ctgcgcgatc tggccggccg tgtggtcgag tcgaccccgc cgcagatgtg gtcccgcatc
    11581 gcccgagagc tggcctcggt ggaggtcacc ccagccaagc gcgaggagtg ggaacagaag
    11641 ttctactggc tgctcgagga ctttcgcttc attcccggtg ggcggattat gcatggcgcc
    11701 ggcaacccca agcgtgtgac gaatctaaac tgctatgtcg ttccggtccg tgatgattca
    11761 atcgagtcg
//
LOCUS       DBNGSB010000085         2766 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_978083, whole genome shotgun sequence.
ACCESSION   DBNGSB010000085 DBNGSB010000000
VERSION     DBNGSB010000085.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_978083"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1479)
                     /locus_tag="AC159B_02405"
     CDS             complement(<1..1479)
                     /locus_tag="AC159B_02405"
                     /inference="COORDINATES: protein motif:HMM:NF014073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR3376639.1"
                     /translation="MLEAFGQGLAAVLQWDAFSFMLIGIALGFWVGLLPGLGGSTTLA
                     LLLPFIYTMQPVQAFAFLLGMHSVTATTGDITSILFGIPGEGTTAASILDGHEMAKKG
                     EAGRALGASLTSSLFGALIGAFALAAAIPLVRPIVLAFGSPEFFMIAILGIAAIASLS
                     GRGPRSMLRGFLAGCLGLMFAAVGSDPQAANLRYTLGTLYLWEGLGLVPVLVGFFAIP
                     EIVDLAVRGSAIAGATPSQRLSGVFQGVKDTFHYWWLTIRCSLVGTYIGIMPGLGGAV
                     AQWMAYAHAVQSAKDPADRDRFGKGDVRGVIGPGAANNSKEGGDLIPTVAFGIPGSSS
                     MAVLLGGFFIVGLVPGPDMLTRHLAVTYSMVWTIILANIITVGVSFLFLNRLAQLTVI
                     RGSVLIPFLTLLIFTGSYTNNNALGDLVVTLFFGLLGYLMVRFHWPRAPLVLGFVLGK
                     VAERYLWISTARYGLEWLGRPIVMILIGLTVLVIALPFIQEWR"
     gene            complement(1559..2563)
                     /locus_tag="AC159B_02410"
     CDS             complement(1559..2563)
                     /locus_tag="AC159B_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3376640.1"
                     /translation="MRAVAVLLLLALIAVLPAQAQQAPPSFAGRTVTIVVGYPTGGGY
                     DRMARLIARHLPRFVPGSPTIIVQNMPGGNSIVAANHVYNTARPDGLTIGAFNRNLVL
                     GQMVNVEGIRFDMTRFAWIGSPSAEATIFAIRSDLPYRTVADMQRADPAVIIGATGPG
                     ASTYDFPLLLKALLKFNVRIIPGYPSGTDILLAVERKEVDGRSGSYSSMKAAIDRGLI
                     RPIIRTRASVPDIARLPVDEDLAPNARAKALFALRSVPEIIGRPYVLPPATPPAIHQM
                     YINAFGQMTKDREFLAEAERSAFDIDYTPGDAALKIVREVLGAPRDVVRVFTQFFNFE
                     "
BASE COUNT          606 a          841 c          847 g          472 t
ORIGIN      
        1 ccgccactcc tggatgaacg gcagcgcgat gaccaggacc gtcagcccga tcaggatcat
       61 cacgatgggg cggccaagcc actccaggcc gtagcgggca gtagagatcc agagatatcg
      121 ctcggccacc ttgccgagca caaacccaag caccagcggc gcacgcggcc agtggaaccg
      181 gaccatgagg tagcctagca gtccgaagaa cagcgttacg acgaggtcac cgagggcgtt
      241 gttgttggtg taggagcccg taaaaatcag cagcgtcaag aacgggatca gcaccgagcc
      301 gcggatcacg gtcagctggg ccaggcggtt gagaaaaaga aagctgacac caaccgtgat
      361 gatgttcgcg agaatgatcg tccagaccat tgaatacgtc acagcgagat gtctggtgag
      421 catgtcaggt ccgggaacca gccctactat gaagaacccg ccgagcagca cggccatcga
      481 cgaactgccg gggatgccga atgccacggt ggggatgagg tctccgccct ctttcgaatt
      541 gttcgccgcg cccggtccga tcacgccgcg cacatcgccc ttgccgaacc ggtcgcgatc
      601 tgccggatcc tttgcgctct ggactgcgtg cgcataagcc atccactgtg ccaccgcacc
      661 gccaagtccc ggcatgatcc cgatgtaagt gccgacgagg ctgcagcgta ttgtgagcca
      721 ccagtaatga aaggtgtcct ttacaccctg aaacacaccg ctcagacgct ggctgggtgt
      781 ggccccggca atcgcgctgc ccctgacagc cagatcaaca atttcaggaa tggcgaagaa
      841 gccgacgagc acgggcacca gaccgaggcc ctcccataag tacagcgttc cgagcgtata
      901 gcgcaaattg gccgcctgcg gatctgaccc tacggcggca aacatcagcc cgaggcaccc
      961 ggcgagaaag ccgcgcagca tcgaccgtgg cccgcgacca ctcagggacg cgatcgcggc
     1021 gatgcccagg atcgcgatca tgaagaattc cggtgatccg aacgccagca cgatcggccg
     1081 gaccagcggg atggccgcgg ccagggcgaa cgccccgata agcgcaccga aaagagaact
     1141 cgtgagggac gcgccgagcg ctcgcccggc ctcgcctttc ttagccatct cgtggccgtc
     1201 aaggatcgac gccgccgtcg taccctcacc ggggatgcca aagaggatcg aagtaatgtc
     1261 gcctgtggtt gcggtgacgg agtgcatccc cagcagaaat gcaaacgcct gcacgggctg
     1321 catcgtgtag ataaacggaa ggagaagcgc gagtgtcgtc gagccgccca ggcccgggag
     1381 cagcccaacc cagaatccaa gggctatgcc gatcagcatg aagctgaagg cgtcccattg
     1441 cagaaccgcc gccagccctt gtccgaacgc ctcgagcatc agttactcct caagacgctt
     1501 gggcctcccc agactgggga ggcccatcgc agccgtcatt gcgagcgaag cgaacgtgtt
     1561 actcgaagtt gaagaactgc gtgaagaccc ggacaacatc cctcggtgcg cccagcactt
     1621 cccggacgat tttcagcgct gcatcgcccg gcgtgtagtc gatgtcgaat gctgagcgct
     1681 cggcttcggc caggaactca cggtccttgg tcatctggcc gaacgcattg atgtacatct
     1741 ggtggatcgc cggaggagtt gccggcggca ggacgtacgg gcggccgatg atctcgggaa
     1801 ctgaacgcag cgcaaagagc gcctttgccc tcgcattggg cgccaggtcc tcatccacag
     1861 gcagccgcgc gatgtcgggc acggaggcac gcgtgcggat gatcgggcgg atcaagccgc
     1921 ggtcgatcgc tgccttcatg gaggaatagg agccggaccg gccgtcaacc tctttacgct
     1981 ccaccgcgag caggatgtcc gtgccggacg gatagccggg gataatccgg acgttgaact
     2041 tcaaaagggc cttcagcagg agaggaaagt cgtaagtgct ggcccccggt ccagtcgccc
     2101 cgatgataac cgcaggatca gcacgctgca tatcggcgac ggtgcgatat ggaaggtcgg
     2161 agcgaatggc aaagatggtc gcttcagccg aggggctgcc gatccaggca aagcgtgtca
     2221 tgtcgaaccg gataccttcg acgttcacca tttgcccgag gaccaggttg cggttgaatg
     2281 ctccgatcgt caatccgtcg ggacgcgcgg tattgtagac atgatttgcc gcgacgatgc
     2341 tgtttccgcc gggcatgttc tgaacaataa tggtcggact cccagggaca aatcttggta
     2401 aatgacgggc aatcaggcgg gccatccggt cgtagccgcc tccggtggga tagccgacaa
     2461 cgatcgtgac cgtgcggcct gcgaaacttg gtggagcctg ctgggcttgt gcaggaagta
     2521 ccgcgatcaa ggcaaggaga agcaatactg caactgctcg cattcacacc acctccgctt
     2581 aacgctttct tccccttgtg tcagggcgtt cctgcggctg tagcggcctt ataatctatg
     2641 ctatcgaagt cttaccattc accattcacg attcactatt cacgtcactt ggaggttcta
     2701 atggagaagc agaccgtccg gcttccagag tcgtgggggc aaaccgcgtt ccgtcgcttc
     2761 ctggag
//
LOCUS       DBNGSB010000086         6231 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_1437177, whole genome shotgun sequence.
ACCESSION   DBNGSB010000086 DBNGSB010000000
VERSION     DBNGSB010000086.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 6231)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6231)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6231
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_1437177"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="AC159B_02415"
     CDS             complement(<1..616)
                     /locus_tag="AC159B_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HIR3376641.1"
                     /translation="MADEQQSPFKRRSFLKLAATIPALGALVALASPLLRFLKPNVEK
                     FQIYAPTAQDIARGEAISVAKLSEVAKPWDFKYFVFSQKYPQYTPEGFKAASVPGVVI
                     RLPSKIELYDPASGGGPLSWARGIGKTPKVSESAVVVFSRICPHLGCIFNYVANYKEV
                     TSGYGGFVPPPNRQHALMACPCHLSIYDPADPQLPGRVLSGPAPR"
     gene            complement(714..1643)
                     /locus_tag="AC159B_02420"
     CDS             complement(714..1643)
                     /locus_tag="AC159B_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3376642.1"
                     /translation="MSIRTLGALAMLIAGTMFSTACQPQVSVSRSGANLQEGLPLEPA
                     AESMVPVRPSAIEGSKVFAKAQCVICHGKEGLGDGPSAANLTAEQKTLLHDLLRLVGI
                     RLAPTQLPSRPANFHNAVAMRLNSPFSMYETVTRGRPHTGMPSFPRPKDYPKKGMPAG
                     RPSYGARLFGVYLTDEERWHVIFHEFSFATTLAELRRGKQLYETKAIPVEVRGVRGTY
                     TCADCHGTKGDGRGARGDELSRSLWGWARGEGPGIFTDINLMAQRKSTELFQPIVDGG
                     RVMPAYRGKLTEDEIWALVNYIWTFVYDYPWPK"
     gene            complement(1694..2344)
                     /locus_tag="AC159B_02425"
     CDS             complement(1694..2344)
                     /locus_tag="AC159B_02425"
                     /inference="COORDINATES: protein motif:HMM:NF024212.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3376643.1"
                     /translation="MQPSSITNSRRRLLELLRTRGAQTIEDLTRGLDLTRSSVRSHLA
                     SLQADGYVVRRGVRTGKRRPSFVYGLTSLADALFPTAYGEFAGMLIEELKREGVRNLD
                     ALLQRVGDRWIARDLPDLEGTSGSERMRRVRDLLAARGFMPDVERTRSGYTLREHNCP
                     LMPLAASHLEICDMVHRWLSALVGTPLQRVQCMRQGDASSAYVTSAPGQSPKTKRH"
     gene            2743..4122
                     /locus_tag="AC159B_02430"
     CDS             2743..4122
                     /locus_tag="AC159B_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014260093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376644.1"
                     /translation="MKSRLALLGVIIVLGLSIVVMRGPWTHLIVPRAGAQAPPQTEFD
                     RGIAQNAQQMMEEGRRIFRFDTFGDEAYWSGTLRMHQVIAGAKNGGVGPGVSPKTALA
                     VGLKVDMDALPAALVEQVRAGQVDLDDPATTLALLKLNAVVGVVGTFREDGSLRAIGL
                     TCAFCHSTVDNAFAPGIGHRLDGWGNRDLNVGAIVALSPNLKAIGDTLGADVATVRKV
                     LTSWGPGRFDAELDKDGKAFRPDGKQAGTIIPNAFGLAGVNLHTWTGAWGTVTYWNAY
                     VANTELRGKGVFYDPRLNDSKKYPVAVKTRSWNVRNSPDLVSSKLGPLHFYQLAIPSP
                     KPPDGIFNTVAAARGKTVFEGKARCATCHVPPLYVEPGWPLHTGKEIGIDEFQARRAP
                     DNRYRTAPLAGLWTRIKGTHPAAGGPGFYHDGRFPTLRAVIDHYDRFFKLRLSEREKN
                     DLVEFLKSL"
     gene            4193..4879
                     /locus_tag="AC159B_02435"
     CDS             4193..4879
                     /locus_tag="AC159B_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3376645.1"
                     /translation="MPAVVADGLTRRFGLVYAVRDLSVSVPRSAVLLVMGPNGSGKTT
                     FLRLLATALVPTAGRASIFGVDVIREPNTVRRTTAYVAAVPGFYAGLTAQENLAFAAA
                     MSSARVEPREWLVRVGLGEVANRPVRTFSQGMKRRLALACAWLRAPTLLLLDEPFSGL
                     DADGRRIVEALIADVKSRNGSAIICTHEWESGMALADHVITLTGGRVVESTPIVSTLY
                     AEFAPARGQR"
     gene            4876..5535
                     /locus_tag="AC159B_02440"
     CDS             4876..5535
                     /locus_tag="AC159B_02440"
                     /inference="COORDINATES: protein motif:HMM:NF015344.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA];
                     GO_process: GO:1903607 - cytochrome c biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1904334 - heme import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HIR3376646.1"
                     /translation="MSGITRAAALARKDLLMEWRGREILTAMATFALLVLLLLGFTIG
                     ATPSTSATILWIALGLATMLGIPRLIYTEVEQGAFETLLLYPGSREHLFWGKSAALAS
                     LLAALWCILLVIAGLLFNLDIWSRLPALVGAGVLGVVGLSATGTLFAALLLHVRGREL
                     LLPVLLLPVMLPLVLAGVRGTEAAMVGGSAGVWVVVIAVFDLLFLLVAPLLFEAITEE
                     T"
     gene            5532..6203
                     /gene="ccmC"
                     /locus_tag="AC159B_02445"
     CDS             5532..6203
                     /gene="ccmC"
                     /locus_tag="AC159B_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HIR3376647.1"
                     /translation="MTVRTVLGALAVALLTTGAYIGLVASPPDAFQGEYVRIMYAHVP
                     NSWIAYLSFGTVFVTSILYLWKRRAIYDQVAHSAAELGVLFTGLALATGMIWGKPVWG
                     TWWTWDARLVTTSILFLIYVGYLLVRALAEDPQRAARAAAVIGIIGFLDVPLLHYSVV
                     WFRTLHQPASIVRGDIKIAPPMLMALMTSLLAFTVLFIYLLTLRVHLQRLDAARSESL
                     ADEVG"
BASE COUNT         1128 a         1924 c         1927 g         1252 t
ORIGIN      
        1 gacgcggagc ggggccggac aagacccgtc ccgggagctg gggatccgcc ggatcgtaga
       61 tactgaggtg acagggacac gccatgagcg cgtgctgccg gttcggcggc gggacaaagc
      121 cgccgtatcc cgaggtgact tccttatagt tagccacgta gttgaagatg cagccgaggt
      181 gcgggcagat ccgtgagaag acgaccaccg cgctctcgga gaccttcggc gtcttaccga
      241 taccgcgggc ccacgagagc ggccctccgc cggaggcggg atcgtacaac tcgatcttgg
      301 agggcagacg aatcacgacg ccgggaacac tcgcggcttt gaacccctca ggcgtgtact
      361 gcgggtactt ttgggagaag acgaagtatt tgaagtccca cggcttcgcc acctccgaga
      421 gcttggcgac ggaaatggct tcgccccgtg cgatgtcctg cgcggtgggc gcataaatct
      481 gaaacttctc gacgttcggt ttgaggaatc gcaagagcgg cgacgccagg gccacgagcg
      541 cgcccagggc gggtatggtc gccgccagct tcagaaaact acgccgcttg aatggagact
      601 gctgctcgtc agccatcgaa ccccaagact tcggattgcg gattgtcgat cgcggatttc
      661 actaacatca tcatatcaaa tccgaaatcg acaattcgaa atccgaaatt gcttcacttg
      721 ggccaaggat aatcgtagac gaaagtccag atatagttca cgagcgccca gatctcgtct
      781 tcggtcagct tgccgcggta agccggcatt acccgtcctc cgtcgacgat gggctggaat
      841 agctccgtcg atttacgctg cgccataagg ttgatgtccg tgaagatgcc cggcccctcc
      901 ccccgcgccc aaccccagag cgatcgtgag agttcgtcgc cgcgagcgcc ccgcccgtct
      961 cccttcgtcc cgtggcagtc ggcgcaggtg tacgtccccc tcacgcccct gacttccacc
     1021 gggatcgcct ttgtctcgta cagctgcttg ccacgcctga gctcagccag tgtcgtggcg
     1081 aaactaaact cgtgaaagat aacgtgccat cgctcctcat cggtgaggta cacgccaaag
     1141 agccttgctc cgtacgatgg acggcccgcc ggcataccct ttttcggata atccttcgga
     1201 cgcgggaagg acggcatccc ggtgtgaggg cggccgcgcg ttacggtctc gtacatgctg
     1261 aacggactgt tcagccgcat cgccacggcg ttgtggaagt tcgccgggcg gctgggcagc
     1321 tgcgtagggg ccaatctgat tccgacgagg cgcaagagat catgcaggag cgtcttctgc
     1381 tcagccgtca ggtttgctgc cgatggtccg tcaccgaggc cttctttacc gtgacagatg
     1441 acgcactgcg ctttcgcaaa caccttcgaa ccctcgatcg cagacggccg gaccggcacc
     1501 atggactcgg ccgcgggctc cagcggcaac ccttcctgca ggttggcgcc gctacgggac
     1561 acgctcactt gtggctggca ggctgtactg aacatcgtcc ccgcgatgag catcgcgagg
     1621 gccccaaggg tccggatcga cataccgcct acgatttctg cgggtgcacc gagtccccct
     1681 cttggatccc taactagtgc cgttttgtct ttgggctctg tcctggcgca gatgtgacgt
     1741 acgcggaact ggcatcgccc tgtcgcatac actgcactcg ctgcagcggt gtgcccacca
     1801 atgcactcaa ccagcggtgg accatgtcgc agatctcaag gtggctcgct gccagaggca
     1861 tcaaggggca gttgtgctcg cgcagcgtgt acccgcttcg ggtccgttca acatcaggca
     1921 tgaacccgcg cgcggcgagc aggtctctca cccgcctcat ccgctcggat ccgctggttc
     1981 cctccaggtc aggcagatcg cgcgcgatcc aacgatcgcc gacccgctgg agcagggcat
     2041 cgagatttcg gactccctct cgcttcagtt cttcaatgag catcccggcg aactcgccgt
     2101 atgccgtcgg aaagagcgca tcagccaaag aagtgagtcc atagacgaaa ctgggccgac
     2161 gcttccccgt ccgtacgccg cgccttacca catagccgtc ggcctggaga gaggcgagat
     2221 ggctcctcac ggagctgcgt gtgagatcca gcccgcgcgt caggtcttcg attgtttgcg
     2281 cgccccgcgt gcgcagaagt tcaagaaggc gacgccgcga attggttatg ctggaaggct
     2341 gcatagacta ccccggccac agtctttcgc ggcatcgcat ctgttacccg caaaacggcc
     2401 acaaatctct cacataggtt tcgaagtgaa ggattatggt ggaagtggta gaaagattca
     2461 ccacaattgt ctttgttgca ttcttgatat ggtcaagggg tatcctcggg gcatgagcgc
     2521 acgccggccc aagcacgctg gccgaatggc cacgtcgcgc cggtcaatcg cgtacagtcc
     2581 ctcgtcaacc tgcgagttgt tcatcgcgct gcccaatcgc ggtttatcca atggggtctg
     2641 ttcccgcgat cgcatgatgt ttgccatgct tgtttccatg ctcctcgaga ctttcgacca
     2701 tgcgttcccg ccctcgatgc tatcttgcgg aggtgagatg caatgaaatc cagactcgcc
     2761 cttcttggcg tcattatcgt cttgggtttg agcatcgtgg tcatgcgggg tccctggacg
     2821 catcttatag tgccgcgggc cggcgcgcag gcgcctccgc aaactgaatt cgatcgcggt
     2881 atcgcacaaa acgcgcaaca gatgatggag gaagggcggc gcatcttccg gtttgacacc
     2941 ttcggcgatg aggcgtactg gagcggcacg ctcagaatgc atcaggtgat cgccggtgcg
     3001 aagaacggcg gcgtgggccc gggcgtcagt ccgaagacgg cgctggccgt gggcctcaaa
     3061 gtggacatgg atgccctgcc cgcggcgctc gtggagcaag tgagagcggg gcaggtggat
     3121 ctcgatgacc cggccaccac actcgccctc ttaaagctca acgcggtggt gggcgtcgtc
     3181 ggcacgttca gggaagacgg cagcctgaga gcgatcggcc tcacctgcgc gttctgccac
     3241 tccacggtcg acaatgcatt tgctcccggc atcgggcacc gattggacgg ctggggcaac
     3301 cgcgacctga acgtcggcgc gatcgtcgcg ctatccccga atctcaaggc gatcggcgat
     3361 acactgggcg ccgatgtagc cacggtccgc aaggtgctga cgagttgggg tccgggcagg
     3421 ttcgacgcgg aactggacaa agacggcaag gcgttccggc ccgacgggaa acaggcagga
     3481 acgatcatcc ccaacgcctt cggcctggcc ggagtcaatc tccacacgtg gaccggggca
     3541 tggggaactg taacgtattg gaacgcctac gtagccaaca ccgaattgag gggcaagggc
     3601 gtcttctacg atccgcgcct caacgacagt aagaagtatc cggtcgccgt gaaaacgcgg
     3661 tcctggaatg tccgcaactc tcctgatctg gtctcctcga agctgggtcc gctgcatttc
     3721 taccagctcg cgatcccctc ccctaaaccg cctgacggga tttttaatac ggtggcagca
     3781 gcccgcggaa agaccgtgtt cgaggggaag gcgaggtgcg ctacctgcca cgtgcccccc
     3841 ctgtatgttg aaccagggtg gccgcttcat acggggaagg agatcggcat tgatgagttc
     3901 caggccagac gggcgccgga caaccgctat cgcactgcgc cgcttgcggg actgtggacg
     3961 cgcatcaaag ggacgcaccc agcggcaggc ggaccgggat tctaccatga cggacggttc
     4021 ccgacgctgc gtgccgtcat tgaccactat gatcggttct tcaagctgag gctgagtgaa
     4081 cgggaaaaga atgacctcgt cgagttcttg aagtcgctct aacaagcaga cgagagaaga
     4141 cggcaaggag tcaagcagga cgcgcgccaa ctatacgagc gcgatggaca ggatgcccgc
     4201 ggtcgtcgcg gacggcctga ccaggcgatt cggcctcgtc tatgccgtcc gcgatttgtc
     4261 tgtatcagtt ccccgctcag ccgttttgct cgtcatgggg ccgaacggat cggggaagac
     4321 gaccttcctg cgactgctgg ccaccgcgct tgtgccgacg gccggtcgcg cctcgatttt
     4381 cggcgtcgac gtgatccgtg aaccgaatac tgttcgccgc accaccgcat acgtggccgc
     4441 agttccaggc ttctacgcgg ggttgacggc gcaggagaat ctggcgtttg ccgcagcgat
     4501 gagcagcgca cgtgtagaac cgcgggaatg gctggtgcgc gttggcctgg gcgaggtcgc
     4561 caatcggccc gtccgcacat tcagtcaggg catgaagcgc aggctggctt tggcctgcgc
     4621 gtggctccgg gcaccgacgc tcttgctcct cgacgaaccg ttcagtgggc tggatgctga
     4681 cggccgtcgc attgtcgagg cgctcatcgc cgacgtcaag tcccgaaacg ggagcgccat
     4741 catctgcacg cacgagtggg agagcggtat ggctctggcc gatcacgtga ttacactcac
     4801 cggaggccgg gtcgtggaat cgactccaat cgtttcaact ctctatgcgg agtttgcccc
     4861 cgcacgagga cagcgatgag cgggatcacc cgagcggcgg cgctggcccg caaggatctg
     4921 ctcatggagt ggaggggccg ggaaatcctg accgcgatgg cgaccttcgc actcctcgtg
     4981 ctgttgctgc tggggttcac gatcggcgca actccgtcga catcggctac gatcctgtgg
     5041 atcgcactcg ggttggccac gatgctcggg ataccccgcc tcatctacac cgaagttgaa
     5101 caaggcgcgt ttgaaacgct tcttctctat cccggaagcc gtgagcattt gttttgggga
     5161 aaatcggccg cgctggcgtc actgctggcc gccctctggt gcatcttgct ggtgatcgcc
     5221 gggctattat tcaatctcga tatttggtct cggctgccgg cgttagtcgg cgcaggtgtg
     5281 ctcggcgtcg tcggcctgtc ggcgacaggt acgctctttg ccgcgttgct tctccacgtc
     5341 cgcggccggg aactcctgtt gccggtgctc ctccttcctg tcatgctccc ccttgtgctg
     5401 gccggggtcc ggggtaccga agccgccatg gtaggaggca gcgctggggt ctgggtcgtg
     5461 gtcatcgcgg tgtttgatct cctgtttttg ctcgtggctc ccctgctctt cgaggccatc
     5521 acggaagaaa catgacggtc cgaaccgtcc tcggcgctct tgcggtcgcc ctcctcacca
     5581 ccggagccta catcggcctg gtggcatctc cacccgatgc atttcagggg gagtatgtgc
     5641 ggatcatgta cgctcacgtg ccgaactcgt ggatcgccta cctgtcgttc ggcactgttt
     5701 ttgtcacgtc catactctat ctatggaagc gccgggccat ctacgaccag gtagcgcaca
     5761 gcgccgccga actcggggtc ctgttcacgg ggctcgcgct cgcaaccggc atgatttggg
     5821 gcaagccggt ctggggcacc tggtggacct gggatgcaag actcgtcacc acctccatcc
     5881 tcttcctgat atacgttggg tatttgcttg tgcgggcgct cgccgaggat ccgcagcggg
     5941 cggcgcgggc agcggcggtg atcgggatca tcgggtttct cgacgtcccg cttctccatt
     6001 actcagtggt ctggttccgc acgctccacc agccggcgtc gatcgtgcgc ggggacatca
     6061 aaattgcgcc accaatgctg atggcgctta tgacgagcct gcttgccttt accgtcctct
     6121 tcatctattt gctcacgttg cgggtgcatc tgcagcggct agacgccgcg cggtcggaat
     6181 ctcttgcgga cgaggtaggg tgatgcctgc tctctggatc gcgctcgcct a
//
LOCUS       DBNGSB010000087         8510 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_4199046, whole genome shotgun sequence.
ACCESSION   DBNGSB010000087 DBNGSB010000000
VERSION     DBNGSB010000087.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 8510)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8510)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8510
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_4199046"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="AC159B_02450"
     CDS             <1..647
                     /locus_tag="AC159B_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008868843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HIR3376648.1"
                     /translation="NGDSEAGLQLAREGKTPVYVYVDGKVAGIVAVADTLKPNSPTVV
                     AELRRMGLEVTMLTGDNRATAEAIGREVGIDRVLAEVLPQHKAAEVKRLQEAGRRVAM
                     VGDGINDAPALAQADLGVAIGAGTDVAIESADIVLVGSDLRGIVTAVQLSRRTMRTIK
                     QNLFWAFFYNIVLIPVAAGALFPFFGILLNPMIAALAMAFSSVSVVANSLRLRF"
     gene            660..863
                     /locus_tag="AC159B_02455"
     CDS             660..863
                     /locus_tag="AC159B_02455"
                     /inference="COORDINATES: protein motif:HMM:NF012621.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HIR3376649.1"
                     /translation="MQMTLKVPKIHCDGCVNTVENAVRKLDGIQNVSASEMSKEVRVE
                     YDPEKVKEEQIRKALLLVGYPPA"
     gene            1061..1136
                     /locus_tag="AC159B_02460"
     tRNA            1061..1136
                     /locus_tag="AC159B_02460"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1094..1096,aa:Arg,seq:tcg)
     gene            complement(1175..1744)
                     /gene="xpt"
                     /locus_tag="AC159B_02465"
     CDS             complement(1175..1744)
                     /gene="xpt"
                     /locus_tag="AC159B_02465"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HIR3376650.1"
                     /translation="MRALHDRILKEGQNLGRGILKVDSFLNHQVDPMLMDECGRELAR
                     RFESVEASKILTAEISGIAPALATALHMALPLVYARRTKPVTMPGQVHMTTAPSHTRG
                     QMIDLMISPEYLTGEDRVLIIDDFLATGQTILGLERLAQAAGAHLVGIGAVIEKVFEG
                     GRDALAGLEVPIEALAIVTDMTGGKIVLE"
     gene            2077..2697
                     /locus_tag="AC159B_02470"
     CDS             2077..2697
                     /locus_tag="AC159B_02470"
                     /inference="COORDINATES: protein motif:HMM:NF048865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR3376651.1"
                     /translation="MAQIHVVRGRRIIALEGNEIVETARASPRVVVDLGTGDGRWLYR
                     LARAHPDWCCIGIDANADAMRESSRRAARKVARGGAPNAWFVLAAVETLPEALFSIAD
                     EVHVQFPWGSLRQMLLSSEKDGLDRVAQLGKHGATFHITLNLLPQDPENYAHFSRVYA
                     RAGLEVERLDLLRVSPETTWGRRIGQGRTMPVLVIEGTFPFPHSSV"
     gene            2716..2791
                     /locus_tag="AC159B_02475"
     tRNA            2716..2791
                     /locus_tag="AC159B_02475"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2749..2751,aa:Lys,seq:ctt)
     gene            2794..2879
                     /locus_tag="AC159B_02480"
     tRNA            2794..2879
                     /locus_tag="AC159B_02480"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2829..2831,aa:Leu,seq:tag)
     gene            3007..4266
                     /gene="tig"
                     /locus_tag="AC159B_02485"
     CDS             3007..4266
                     /gene="tig"
                     /locus_tag="AC159B_02485"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIR3376652.1"
                     /translation="MKVEVKKEPPSRAVLEVELPPEDLQKGLEEALTRLNRRVTIPGF
                     RRGRAPKVLLQRFVGKDTVYEEAVNLLVPDAYASAVDQAGVRPITRPQIQVESVEEGK
                     PLRFTATVDLVPEVSLGDYRAIRIPTERAAVTDADVESAIEDLRRRNAQLVALEGRPA
                     AQGDYVLGRVVEAAGDQARLQPGKEYLIEIGGGTYPEEVERSLVGVSVGERKTMTTTD
                     PASTVTLEVSGLKRRELPELSDEFAKTAANAATIQELRDLLRTRMQQEVEERVRQAYQ
                     EKVVDALLQSATIDVPSSLIEHELQHLVGDLTETLSRRGVTLQRYLQATEKTEQQMLD
                     ELRPSAERRIRTELAIDEFSRQDGVTPSQEEIDREVENVARRLQLDDARVKEWLGEQG
                     RFDSLVTALRRRQALSRLVAIARGDAA"
     gene            4263..4874
                     /locus_tag="AC159B_02490"
     CDS             4263..4874
                     /locus_tag="AC159B_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HIR3376653.1"
                     /translation="MTLVPMVVEQTARGERAYDIYSRLLKDRIVFVGGPIDDDMANLV
                     IAQLLFLEADDPEKEINLYINSPGGSLTPALAIYDTMQYVRPQVVTICVGLAASGAAV
                     ILAGGARSKRFALPYSQILIHQPWGGAQGTTADIDIQAKQFLKMRQLTNEILAKHAGQ
                     PIERIERDTDRDYWMDPQTAKEYGLIDEVFQPREKVVAAVRDR"
     gene            4897..6165
                     /gene="clpX"
                     /locus_tag="AC159B_02495"
     CDS             4897..6165
                     /gene="clpX"
                     /locus_tag="AC159B_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007952676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HIR3376654.1"
                     /translation="MFRFGDGDKLKCSFCGKSQEQVRKLVAGPGVYICDECIELCNEI
                     IEEELYGDSEPSAPLPRHTPKPREIYETLCQYVVGQDRAKKALAVAVYNHYKRIGSRR
                     PTDVELAKSNILLIGPTGCGKTLLAQTLAKILDVPFAIADATSLTEAGYVGEDVENIL
                     LRLIQAADFDVKKAERGIIYIDETDKIARKSENPSITRDVSGEGVQQALLKILEGTVA
                     NVPPQGGRKHPHQEFIQIETTNILFVCGGAFEGLDRIIESRIRQTTIGFGAHIVPKSK
                     ASLSETLSHVMPQDLLRYGMIPEFIGRLPIIVPLEPLDEEDLVRILVEPKNALVRQYQ
                     KILEMDGVELSFTDEALRAISREAMTRNTGARGLRTIIEDIMLNIMYDIPSRPDVKRC
                     VVTEEAVLHRREPLLLTSAEVKKMAKEKPA"
     gene            6323..>8510
                     /gene="lon"
                     /locus_tag="AC159B_02500"
     CDS             6323..>8510
                     /gene="lon"
                     /locus_tag="AC159B_02500"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIR3376655.1"
                     /translation="MTERIEVTAKDIKDVLPLLPLKGTVILPYQVAPLGVGRQKSLKA
                     LEAALAGDRILVLVAQKQDDIEDPTGEDLRNVGTVCRVVQVGRQPDGVLQVIVEGVMR
                     AEIVSVEQDSPHFEARIATRPDPAEKNLELEAHMRGVVSQFERFARFSRAVAPEQYAQ
                     VMQTEEPGKLADLVAQHLPVKLDERQQLLEKNPKERLDLLSQILSREVSILELERKIQ
                     NRVRKQMEKSQREYFLKEQMKAIQQELGEADERASEVDEYKKKIEAANLPDHVKEKAL
                     EEVARLEKMPPMVAEAVVVRTYLDWILATPWTVKTEDRLDIQEARRILDEDHYGLDKP
                     KERVIEYLAVRKLAPASKGPILCFIGPPGVGKTSMGKSIARALGRKFVRISLGGVRDE
                     AEIRGHRRTYVGALPGRIVQGLKTAGTKNPVFMLDEIDKLGVDWRGDPSAALLEALDP
                     EQNHSFSDHYLELPLDLSEVMFICTGNILDTVPPALRDRLEVIRFPGYIEEEKIKIAE
                     QFLIPKQRSQNGLKPEHIVFVHEALRRISREYTREAGVRNLEREIAAICRKVATEVAT
                     ERAKSVRVTMQNLHKFLGPPKFRYGIVEKDDEVGIAMGLIYSEMGGDIVSVEATLMRG
                     DGKLQLTGQLGDVLKESGQAALSYVRSRARRLNIDEEFYQKFDVHIHIPAGAVPKDGP
                     SAGITLATALASAVSGRAVRNVVAMTGEVTLRGKVLPIGGVKEKVLA"
BASE COUNT         1788 a         2464 c         2750 g         1508 t
ORIGIN      
        1 tcaatgggga cagcgaggcg ggattgcagc ttgcacgcga ggggaagacc cctgtctacg
       61 tctacgttga cgggaaagtt gcagggatcg ttgccgtcgc tgacactctg aagccgaact
      121 caccgacggt cgtggcggag ctcaggagaa tgggccttga ggtgaccatg cttaccggcg
      181 acaatcgcgc caccgccgag gccatcggac gtgaggtcgg gatcgacagg gtcctcgcgg
      241 aggttctgcc gcagcacaag gcagcggaag tgaagcggct gcaggaggcg gggcgccgtg
      301 tggcgatggt cggcgatggc atcaacgatg cgccggcgct cgcgcaggca gacctgggag
      361 ttgcgatcgg cgccggcacc gacgtggcga tagagtcggc agatatcgtg ttggtgggct
      421 cagatcttcg aggcatcgtg accgcagtgc agttgagcag gcggacgatg cggacgatca
      481 aacagaacct gttctgggca ttcttctaca atatcgtgct catcccggtg gcggcaggcg
      541 cgctcttccc gttcttcggg atcctcttga atccaatgat cgctgcactt gccatggcgt
      601 tcagttccgt gtctgtggtc gcaaatagcc tgagactgcg attctgaggg gagagaaaca
      661 tgcagatgac tctgaaggta cccaagattc actgtgacgg gtgcgtcaac accgtagaga
      721 atgccgtgcg gaaactggat ggcattcaga acgtgtcggc gagtgagatg tccaaagagg
      781 tcagggtcga atacgatccg gaaaaggtta aggaagagca gatccgcaag gctctcctgc
      841 tggtcgggta tccacccgcg tagcgacgcg gactgatgcc aaaagccgat acacccgatc
      901 cgcactctga cgcccgcgaa cgcaaacgga agaaagcccg gtatggtatg cgagtgactg
      961 ggagatcggt gcgattgctc gcaaaactca cgaccgaccc ctcgcggaaa aagccgccga
     1021 agaagaagaa atcacgtgcg cgtgctaaaa tgatgcgcgt gcgcccgtag cttaacggat
     1081 aaagcaccgg gcttcggacc cggggagtgg gggttcaaat ccctccgggc gcgccatgca
     1141 ccactcgcgc cgctcggatg catggcaacg ccagttactc gagaacgatc tttcccccgg
     1201 tcatgtcggt gacgatcgcc agtgcctcga tgggcacctc gagccccgcc agcgcgtcgc
     1261 ggccgccttc gaacactttc tcaatcactg cgccgatacc aaccaggtga gcgccggccg
     1321 cctgcgccag tcgctcgagc ccaaggatgg tctgaccggt tgccaggaag tcatcgatga
     1381 tcaggacgcg atcttcgccg gtgaggtatt cgggtgaaat catcaggtca atcatttgac
     1441 ctcgcgtgtg agacggtgcc gtcgtcatgt ggacttggcc gggcatggtg actggtttcg
     1501 tgcgacgggc gtagacaagc ggcagcgcca tatgcaacgc tgtggccagc gccggcgcga
     1561 tcccggagat ctccgctgtc agaattttcg aagcctccac cgattcgaac cgcctggcca
     1621 gctcgcgtcc gcattcgtcc atcaacatcg ggtctacctg atggttaagg aagctgtcga
     1681 ccttgaggat gccgcgtcca aggttctgac cttctttcag gatgcggtcg tgaagggctc
     1741 tcacgcggac ctccggcgaa cagattcatt caccgattgg cggcttgcgt ccaacgttcc
     1801 tggctaaaac tgacatgcat ggggccttgg cccctattgg taaggactta gctagttgaa
     1861 aagcctcgcc taggttatac tgaggtcgaa atgcgtccct tcggctgagc ttagctagct
     1921 cgatccaacc aatctagcgc cagttctctt cagtctgaga agaaactatt ctcgtgtgcc
     1981 cgcggggctg taccccggcg ggcttttgta tttggacaca tccgcgggcc tccgtgcctc
     2041 cctgcattct taccagatcg caggggggtg gaggagatgg ctcaaattca tgtggtgcgt
     2101 ggacgaagaa tcatcgcact tgaaggaaat gaaatcgtgg aaacggcgcg cgcttcgccg
     2161 cgggtcgtcg tcgatctcgg taccggcgat ggacggtggc tgtatcgttt ggcgagagcc
     2221 catccggact ggtgctgtat tggcatcgac gcaaatgctg acgccatgcg cgagtcatcg
     2281 cggcgggcgg cgaggaaggt cgccaggggc ggagcgccca acgcctggtt cgtgttggcg
     2341 gcagtcgaga cgctgccgga agcattgttt tccatcgccg acgaggtgca cgtccaattt
     2401 ccgtggggaa gtttgagaca gatgctactg tcgtcagaaa aggacggact cgaccgagtc
     2461 gctcaactcg ggaaacatgg ggcaacattc catatcacgc tcaatctact tccgcaggat
     2521 cctgagaatt acgctcattt ctctcgcgtc tacgctcgag ctggtcttga ggtggaaagg
     2581 ctggaccttt tgagagtttc tccagagacg acctggggtc ggcgcatcgg tcagggtagg
     2641 acgatgcccg ttctggtcat cgagggcacg ttcccttttc cacattcatc ggtataatga
     2701 aacaggaaat cgtgcgggcc ggtagctcaa ctggcagagc agcggactct taatccgctg
     2761 gttgagggtt cgagtccctc ccggcccccc atcgcgggcg tggcggaacc cggcagacgc
     2821 gcgagattta ggatctcgtg gggtaacccg tgggggttca actccctccg cccgcaccaa
     2881 acgagtcaaa gggcccggat gggcccttcc tatttccctc cccgtttgcg ggagggggga
     2941 caaagttcca taaaagttct gcgaatgatc tccaattagc ctgtccgcgt ctaacgaggt
     3001 gaggacgtga aggttgaagt gaagaaggaa ccgcccagcc gcgctgtcct tgaggtcgaa
     3061 ttgcccccag aggacctcca gaagggactg gaggaagcgc tgacacgcct caacaggcgt
     3121 gttaccatcc cggggttccg gcgcgggcgg gcaccaaagg tgcttcttca gcgttttgta
     3181 gggaaagata ccgtgtacga ggaggcggtc aaccttctgg tccctgatgc atacgccagc
     3241 gcggtcgacc aagctggggt ccgcccgatc actcgaccgc agattcaggt cgagtcggtg
     3301 gaggaaggca agccgctgcg ctttacggca acggtcgacc tggttccgga ggtttcgctc
     3361 ggagattacc gggcgatccg gattccgacc gagcgggcgg cggtcaccga cgccgacgtc
     3421 gaatcggcta tcgaggatct tcggcggcgg aatgcgcagt tagtggcact cgaagggcgg
     3481 cctgcagcgc aaggagacta cgtgctgggc cgtgttgtgg aggctgcagg agatcaggct
     3541 cggctccaac ctgggaagga atacttgatc gaaatcggcg ggggtacgta cccggaagag
     3601 gtggagcgct cgctcgtcgg tgtgtccgta ggagagcgaa aaactatgac gacgactgac
     3661 ccggcgtcca cagtgacact cgaggtctcc ggcctcaagc gcagggagct ccctgagctg
     3721 tcggacgagt tcgcgaagac agccgcgaac gccgcgacga ttcaggagct tcgggacctc
     3781 ctccggacgc gcatgcagca ggaggtagaa gagcgggtca ggcaggcgta ccaggagaag
     3841 gtggttgacg cgctcctcca gtcggcgacc attgatgtcc cgtcgagcct cattgaacat
     3901 gagctccagc acctggtcgg cgatctgacc gaaaccttgt cccggcgcgg tgtgacgctg
     3961 cagcgctacc tgcaggcgac agagaagacc gagcagcaaa tgctggatga gctgcgcccg
     4021 tcggcagaac gccgcatccg cacagagctg gcgatcgacg aattttctcg tcaggatggc
     4081 gtcacaccca gtcaggagga gatcgatcgc gaagtagaga acgttgcccg caggttgcaa
     4141 ctcgacgacg ctcgcgttaa ggaatggttg ggcgagcagg gtcgtttcga ctcattagtg
     4201 accgcactca ggcgccggca agccctctcc cgcctggtcg ccatcgcacg aggtgacgca
     4261 gcatgacgct tgtccccatg gtcgtcgaac agaccgcgcg cggcgagcgc gcctacgaca
     4321 tttactcacg tttgctcaaa gaccgcatcg tgttcgtcgg cggcccgatc gacgacgata
     4381 tggcgaatct ggtgattgcg cagctcctgt ttttggaggc cgatgacccg gagaaggaga
     4441 tcaacctcta tatcaacagc ccgggcggca gcctgacacc ggcgctcgcg atctacgaca
     4501 cgatgcagta cgtgcgtcct caggtcgtca cgatctgtgt cggtctcgcg gcaagcggcg
     4561 ccgccgtgat cctggccgga ggggcgagga gcaagcggtt cgcgctgccg tactcgcaga
     4621 tcctcatcca ccagccgtgg ggtggggcac aggggacgac cgcggacatc gacatccagg
     4681 cgaagcagtt tctgaagatg cgccagctca cgaacgagat cctggccaag catgctggcc
     4741 agccgatcga gcgcatcgag cgcgataccg accgcgatta ctggatggat ccgcagacgg
     4801 ccaaagagta cggtctgatc gacgaagtgt ttcagccgcg tgagaaggtc gttgcggccg
     4861 tgagggatcg ctaggggcac aatgaccaaa gagcggatgt tccggtttgg cgacggcgac
     4921 aagctgaagt gctccttctg cggtaagtcg caggagcagg tgcgcaagct cgtcgcgggg
     4981 cccggtgtct acatctgcga tgagtgcatc gagttgtgca atgagatcat cgaggaagag
     5041 ctctacggag actccgaacc ctccgcgccg cttccccggc acacaccgaa gcctcgggaa
     5101 atttatgaaa cgctctgtca atatgtcgtt gggcaggatc gtgcgaagaa agccctcgcg
     5161 gtcgcggtgt acaaccatta caagcggatc ggctcgcgcc gcccgaccga cgtggagctg
     5221 gcaaagtcca acatcctgtt gattggaccg accggctgcg gcaagacact gctcgcgcag
     5281 accctcgcga agatcctcga cgtgccgttt gcgatcgccg acgcgacatc gctcaccgaa
     5341 gccggttatg tcggcgaaga cgttgagaac atcctcctgc ggctgataca ggcggcggac
     5401 ttcgacgtca agaaggccga gcgcggcatc atctacatcg acgagaccga caagattgcc
     5461 cgcaaatcgg agaacccgtc catcacacgc gacgtctccg gcgagggcgt gcagcaggcg
     5521 ctgctcaaga tcctcgaggg cacggtcgcg aatgtgcctc cccagggcgg ccgtaagcac
     5581 ccacaccagg agttcatcca gatcgaaact accaatatcc tctttgtctg cgggggcgcc
     5641 ttcgaggggc tggatcgcat catcgagtca cgcatcaggc agacaacgat cgggttcggc
     5701 gcgcacatcg tcccgaagag caaggcgtcg ctctccgaga ccctctcgca cgtgatgccg
     5761 caggacctgc tgcggtacgg catgatccct gagttcatcg gccggctgcc tatcatcgtc
     5821 ccgctcgaac cgctcgacga ggaagatctg gtccggatcc tcgtggaacc caagaacgcc
     5881 ctcgtccggc agtaccagaa gatactggag atggacggcg tggagctgtc gtttaccgat
     5941 gaggccttgc gcgcgatctc tagagaggcc atgacgcgga acacgggcgc ccgcggcctg
     6001 cgcacgatca tcgaagacat catgctgaat atcatgtacg atatcccgag ccggccggat
     6061 gtgaagcgct gcgtggtgac cgaagaagcc gtactgcatc gccgggaacc gctgctgctg
     6121 acctccgcgg aagtgaagaa gatggcgaag gagaaaccgg cctagacgcc gcgtggtaaa
     6181 atatacgtag aacgaagata tggccgcacg gcggccgtga cgagacgaag gcgaaggggg
     6241 gacggttcgt acccccttcg ttgttagaaa cggccgggaa cagatcgacc cggccgctcg
     6301 ttcgagatgt gggaggaaac tcatgactga acgcattgaa gttacagcca aagacatcaa
     6361 agacgttctg cccctgctgc cgctgaaggg gactgtcatt ctgccttatc aggtggcccc
     6421 gctcggcgtg ggcagacaga agtcactgaa agcgcttgaa gcagcgctcg ccggtgatcg
     6481 cattctggtg ctcgtggcgc agaagcagga cgacatcgag gatccgacgg gcgaagacct
     6541 tcgcaacgtc ggcacagtgt gccgcgtggt gcaggtgggc cgccagccgg acggcgtcct
     6601 gcaggtcatc gtcgagggcg tgatgcgcgc cgagatcgtt tcggtggagc aagattcccc
     6661 gcatttcgaa gcgcggatcg cgaccaggcc tgatcccgcg gaaaagaacc tcgaactcga
     6721 agcgcacatg cgcggcgtgg tgtcacagtt cgagcgcttc gcgcggttta gccgcgcggt
     6781 ggcgccggaa cagtatgccc aggtcatgca gaccgaagag ccgggcaagc tcgccgacct
     6841 cgtggcgcag cacctgcccg ttaagctcga cgagcgtcag cagctcctgg agaagaatcc
     6901 caaggagcgg ctcgatctgc tgagtcagat cctctcgcga gaagtcagca tcctcgagct
     6961 ggaacggaag atccagaacc gcgtgcgcaa gcagatggaa aagtcccagc gcgagtactt
     7021 cctcaaggag cagatgaaag ccatccagca ggaactgggg gaggctgacg agcgcgcaag
     7081 cgaggtcgac gaatacaaga agaagatcga ggccgcgaac ctgcccgacc acgtcaagga
     7141 gaaggcgcta gaggaagtcg cgcggctgga gaagatgccg ccgatggtcg cggaagcggt
     7201 ggtcgtccgc acctacctag actggatcct cgccacgccg tggacggtga aaactgaaga
     7261 ccggctcgat attcaagagg ctcgaaggat cctcgacgag gaccactacg gcctcgacaa
     7321 gcccaaagag cgtgtgatcg agtacctcgc ggtgcgcaaa cttgcgcctg ccagcaaggg
     7381 accgatcctg tgcttcatcg gtccgccggg cgtcggcaag acatccatgg gcaagtcgat
     7441 cgcgcgcgcc ctcggccgca agtttgtgcg gatcagcctg ggcggcgttc gcgatgaggc
     7501 ggagattcgc ggccaccggc gcacgtatgt aggcgcgctg cccggtcgca tcgttcaggg
     7561 cctcaagacc gccggcacga agaacccggt gttcatgctc gacgagatcg acaagctcgg
     7621 tgtggactgg cggggcgacc cgtctgcggc actactggag gcgctcgatc cggagcagaa
     7681 ccacagtttc agcgatcatt acctggagtt gccgctcgac ctgtctgagg tgatgttcat
     7741 ctgcacgggc aacattttgg atacggtccc gccggcgctg cgcgatcgcc tggaagtcat
     7801 ccgcttcccc gggtatatcg aggaagaaaa gatcaagatc gccgagcagt tccttattcc
     7861 caagcagcga agccagaacg ggctgaaacc agagcacatc gtgttcgttc atgaagcgct
     7921 ccgccggatc tcgcgcgagt atacgcgcga ggccggagtg cggaatctcg agcgagagat
     7981 cgcggcgata tgccggaaag tggcgacaga agtcgcgacc gagcgagcga aatccgtgcg
     8041 cgtcacaatg cagaacctgc ataagttcct cggcccgccg aaattccgat acgggattgt
     8101 cgagaaggac gatgaggtcg ggatcgccat gggcctcatc tactccgaga tgggcggcga
     8161 catcgtgtcg gtcgaagcca cgctcatgcg cggagacgga aagctgcagc tcaccggaca
     8221 actcggcgac gtgctgaagg agtccggcca ggcggcgcta tcgtacgtcc ggtcgcgcgc
     8281 ccgccggctg aacatcgacg aggagttcta ccagaagttc gacgtacaca ttcacatccc
     8341 agcgggcgcg gtgccgaagg acggcccgtc ggccggcatc acgctggcca cggcgctcgc
     8401 ctcagcggta agcggacgcg cggtgcgcaa cgtggtcgcg atgaccggcg aagtcacgct
     8461 ccgcggcaaa gtcttgccga ttgggggcgt gaaggaaaag gtcctggcag
//
LOCUS       DBNGSB010000088         4326 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2665159, whole genome shotgun sequence.
ACCESSION   DBNGSB010000088 DBNGSB010000000
VERSION     DBNGSB010000088.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4326)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2665159"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /gene="sufB"
                     /locus_tag="AC159B_02505"
     CDS             <1..828
                     /gene="sufB"
                     /locus_tag="AC159B_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007239450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HIR3376656.1"
                     /translation="GVHVDIPLQAYFRINAENMGQFERTLIICEPGSYVHYVEGCTAP
                     IYTTDSLHSAVVEIIVKEGARCRYTTIQNWSTNTYNLVTKRAVAYRDATMEWIDGNLG
                     SKITMKYPSVFMVEPGAKAEILSVAFAGKGQHQDPGGKVIHAAPHTQSVIVSKSIAKD
                     GGRAGYRGLVKIYDGAKGSKSAVRCDALILDEHSRSDTYPTMEIDEQDVSITHEATVS
                     KVSDEQLFYLMSRGVKADEAMNMIVRGFVEPIVKELPLEYAVELNRLIQMEMEGSVG"
     gene            960..1382
                     /locus_tag="AC159B_02510"
     CDS             960..1382
                     /locus_tag="AC159B_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376657.1"
                     /translation="MAETGQVVEAGQFTRRAVEAISERHDEPKWVRDARLRAFETFER
                     LPWPTTREEKWRRTDLKDLHSETLAPYASAPQHTVPAVAGEAIKNAGARGGAVVQWNS
                     ETVASDLVPELASRGVVLTDVHAALRDHPGLFRDHFMQ"
     gene            1425..2315
                     /gene="sufD"
                     /locus_tag="AC159B_02515"
     CDS             1425..2315
                     /gene="sufD"
                     /locus_tag="AC159B_02515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HIR3376658.1"
                     /translation="MWSGGTFVYVPDRVEVELPLLSYTWVDAEGLAIFPHTIVIAGEG
                     SRVTVVDGFSSLTGRSQFLAVPVVELILGDGAQVRFVTAQDWGRNAWEIGMIRSSLSR
                     DSTLNSLLVGLGGAVVRTDVESVLRGPGASSEMLGLIFGDGRQRYEVQTLQEHAAPHT
                     TSDLLYKNAVKDWASSVFIGMIRVHPGAQKTNAFQVNRNLMLSDSSRAISDPKLEIMA
                     NDLRCTHGSATSRLNEEHLFYLMSRGLTRQGAIRMVVEGFFAELLDRVPMEWLTTRLQ
                     SVIAAKMMHEQEPEASSKRQ"
     gene            2321..2665
                     /gene="nirD"
                     /locus_tag="AC159B_02520"
     CDS             2321..2665
                     /gene="nirD"
                     /locus_tag="AC159B_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HIR3376659.1"
                     /translation="MAEYVKVAKIEEIPAGQGKAVTVDGRQIAIFNVEGAFYAIDDTC
                     THEEASLAEGAVYGDIVACPKHGSRFHLSTGRVLSLPAVIPVNTYPVKVEDGQVLVLP
                     EPQRGRGMPHKV"
     gene            2676..3923
                     /locus_tag="AC159B_02525"
     CDS             2676..3923
                     /locus_tag="AC159B_02525"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - obsolete cysteine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HIR3376660.1"
                     /translation="MIPANVRDEFPILKQKMHGKPLIYLDSAATSQKPQRVIDALVRY
                     YQEYNANVHRGIYHIAEEATRRYEASRGKVAGLINARRPEEIIFTRSTTEAINLVAYA
                     WGRANVREGDEIITTEMEHHSNLVPWQMLAAERNARLLFVPFDDQVLLRLDEFDRLLT
                     ERTKLVALTHQSNVLGTINPVKDITRKAHAVGAKVLIDGAQSAPHMPVDVQDLGVDFF
                     ALSAHKMCGPTGAGAIWARYEILDAMPPFHGGGEMIMLVQLDKSTYKEPPHKFEAGTP
                     NIADCIVWGDAIDYLQAIGLDAIRAHEKQLTAYALSKLSEVPGMKTFGPRDLERRGGV
                     VSFHLDVAHPHDVAQVLDQDGIAVRAGHHCAQPLHRRLGVSASTRASFYLYNNEEDLD
                     ALVRGLHVVSRMFSPRPATVPGS"
     gene            4060..>4326
                     /locus_tag="AC159B_02530"
     CDS             4060..>4326
                     /locus_tag="AC159B_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376661.1"
                     /translation="MAQQIGSSVASAPPAVSSSQVFATVMQRFLAQVVDALVAFGLFY
                     FTGMQVARRFGGLTTTGFEMTGRPAALAIGITAAFLLAYYVFAEA"
BASE COUNT          859 a         1278 c         1379 g          810 t
ORIGIN      
        1 ggcgtgcacg tcgacatccc gctgcaggcc tatttccgaa tcaatgcgga gaatatgggc
       61 cagttcgagc ggacgctgat tatctgcgag cccggttcat atgtgcacta cgtggaaggc
      121 tgtacggcgc cgatctacac gaccgattcg ctgcacagcg cggttgtgga gatcattgtg
      181 aaagaaggcg cgcgctgccg ctatacgacg atccagaact ggtccacgaa cacttacaac
      241 ctggttacta agcgggccgt cgcctaccgt gacgcgacga tggaatggat cgacggcaac
      301 ctgggctcca agatcaccat gaagtacccg agcgtgttta tggtggagcc gggcgcgaag
      361 gctgagattc tctccgtcgc gtttgcgggc aaggggcagc accaggatcc gggcggcaaa
      421 gtgatccacg ccgcgccgca cacacagtcg gtgatcgtga gcaagtccat cgccaaggac
      481 ggcggccggg caggataccg cgggctcgtg aagatctacg atggcgcgaa aggcagcaag
      541 tctgcggtgc gctgtgatgc gctcatcctc gacgagcata gccgcagcga cacgtatccc
      601 acgatggaaa tcgacgagca ggatgtcagc atcacccacg aggctaccgt ctccaaggtg
      661 tccgacgagc agttgttcta cctgatgagc cggggcgtga aggcggacga ggccatgaac
      721 atgatcgtgc gaggcttcgt cgagccgatc gtcaaggagc tgcccctcga gtacgcggtg
      781 gagctcaacc ggctcattca aatggagatg gaagggtcgg tgggctaagg ctctaggctg
      841 taccgcggct ggtagggaac ggagcccctc cccgactgaa actgatacgg ggacccctta
      901 ggctccgtcc cccaccagcc gcttttcatg atatagggct ggtcctaggc ttaggtggaa
      961 tggcagaaac agggcaggtc gtggaggctg ggcagttcac gcgaagggcc gttgaggcga
     1021 tctctgagcg ccatgacgag cccaaatggg tgcgagatgc gcgcctgcgg gcgtttgaga
     1081 cattcgagcg cctgccctgg cctactacgc gcgaagagaa atggcggcgc accgacctga
     1141 aggacttgca ctcagagacg cttgcaccgt acgccagtgc tcctcagcat acggttcccg
     1201 ctgtcgccgg tgaggcgatt aagaatgccg gcgcgcgtgg cggcgcggtg gtgcagtgga
     1261 actccgagac ggtagcgtcc gatctcgtcc ccgagctcgc atctcgagga gttgtactca
     1321 cggacgtcca tgccgcgctg agagatcacc ccggcctgtt tcgtgaccat ttcatgcagt
     1381 aagtccgccc cgacgaatcg aagttcaccg cgctccattc cgcattgtgg agcggcggga
     1441 cattcgtcta cgtacctgac cgtgtcgaag tcgagctgcc gctcctgtcc tacacgtggg
     1501 tcgatgccga gggcctcgcg atttttccgc atacgatcgt catcgccggt gagggaagcc
     1561 gcgtcacggt cgtggacggg ttctcatctc tgaccggccg gagccagttc ctggcagttc
     1621 ccgtggtgga gctcatcctg ggtgatggcg cacaggtgcg atttgtgacc gcgcaggact
     1681 ggggccggaa cgcatgggag atcggcatga tccgctcttc cctgagccgc gactctacgc
     1741 tgaacagcct gctggtgggc ctgggtgggg cggtcgtgag gaccgatgtc gagtcggttt
     1801 tgcgtggccc gggcgccagt tcggagatgc tcggcctcat ctttggtgat gggcggcagc
     1861 gctatgaggt tcagacactg caggagcatg ccgcgcccca caccacgagc gatctcctgt
     1921 acaagaacgc cgtcaaggat tgggcgagca gcgtcttcat cgggatgatt cgcgttcacc
     1981 cgggggcgca gaagaccaat gcctttcagg tcaaccgcaa cctcatgctt tccgatagtt
     2041 cccgggcgat ctccgatcca aaactggaga tcatggccaa cgacctgcgc tgcacgcacg
     2101 gatcggcgac gagccgcctt aacgaagagc atctgttcta cctgatgagc cgtggactga
     2161 ccaggcaggg tgccatacgg atggtagtgg agggattctt cgcggaattg ttggaccgcg
     2221 taccgatgga gtggctcacg acccgactgc aatcggtgat cgcggcgaaa atgatgcatg
     2281 agcaggagcc cgaggcttca tcgaagcgtc aatgagagag atggctgagt acgtaaaggt
     2341 tgccaaaatc gaggagattc cggcggggca ggggaaagcg gttacggtag acggccgcca
     2401 gatcgcgatc ttcaacgtcg agggtgcctt ctacgccatc gacgatactt gtacgcatga
     2461 ggaagcctcg ctggcggagg gagccgtgta cggcgacatc gtggcctgcc ccaagcacgg
     2521 ctcgcgcttt cacctttcca ccggacgagt gctgtcgctg cctgccgtga ttcccgtcaa
     2581 cacgtatccg gtgaaagtcg aggacggtca ggtattggta ttgcccgaac cacagcgcgg
     2641 gcgtggaatg cctcacaagg tgtagcgagg acgtcatgat accggcaaac gtgcgagacg
     2701 aattcccgat cctcaagcaa aagatgcacg ggaaaccgct catctacctc gacagcgccg
     2761 caacctctca gaagccacag cgcgtgatcg atgcacttgt ccgctactac caggagtaca
     2821 acgccaacgt tcatcgcggt atctatcaca tcgccgagga ggcaacgcgg cggtatgagg
     2881 cgtctcgtgg caaagtggca ggactgatca acgcgcggcg tcctgaggag atcatcttca
     2941 cgcgaagcac gaccgaggcg atcaacctgg tggcctacgc gtggggccgg gcgaacgtcc
     3001 gcgagggcga cgagattatc accaccgaaa tggagcacca cagcaacctt gtgccctggc
     3061 agatgttagc agccgagcgg aacgctcggc tgctgttcgt tccgtttgat gatcaagtac
     3121 tgctgcggtt ggacgagttc gatcgcttgc tgaccgagcg caccaaactg gtggccctga
     3181 cgcaccaatc gaacgtgctg gggacaatca accctgttaa ggacatcacc cggaaggcgc
     3241 acgcggtcgg agccaaggtg cttatcgatg gggcacagag cgcgccgcac atgccggtag
     3301 acgtgcagga cctcggtgtc gatttcttcg ccctctcggc gcacaagatg tgcggaccga
     3361 ccggggccgg ggcgatctgg gcccggtacg agatcctgga cgccatgccg ccattccacg
     3421 ggggcggcga gatgatcatg ctggtccaac tcgataaatc gacgtataaa gagccgccgc
     3481 ataagttcga ggcggggacg cccaatattg ccgattgcat cgtgtggggg gacgcgatcg
     3541 actacctcca ggccatcggg ctggacgcaa tcagagcgca cgagaaacag ctgacggcgt
     3601 acgcactatc taagctgagt gaggttccgg gcatgaagac cttcggtccc cgcgatctcg
     3661 agcgccgggg cggggttgtg tcattccatc ttgacgttgc gcacccccat gatgtggccc
     3721 aggtcctcga ccaggatggc atcgccgttc gcgccggcca ccactgcgcg cagccgctgc
     3781 accgccggct cggcgtgtcg gcgtcaaccc gtgcgagctt ttacctctac aacaacgagg
     3841 aggatttgga cgccctcgtg cggggtcttc acgtggtaag ccgcatgttc tctcctcgcc
     3901 cggcaacggt ccccggttct tagccctctc ccaggagttc gttagacagg ctccgaaccc
     3961 acgagttaat cccagacaca accgcattcc tgaccgtggc ctgccgttca ggtcgactgt
     4021 tggcttaaac tgctaaacag gaaagagagg cgacgtgggt tggcccaaca gatcggctca
     4081 tcggttgcct cggcaccgcc ggcagtctca tcctcgcagg tgtttgccac tgtcatgcag
     4141 cggttcctcg cgcaggttgt agacgcgttg gttgcgttcg gcctgttcta tttcaccggc
     4201 atgcaggtcg ccaggcggtt cggcggactg actaccactg ggttcgagat gaccggccgc
     4261 cccgcagcct tggccatcgg gatcaccgcc gcctttctgc tcgcgtacta cgtgttcgcc
     4321 gaggcg
//
LOCUS       DBNGSB010000089         3807 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2820780, whole genome shotgun sequence.
ACCESSION   DBNGSB010000089 DBNGSB010000000
VERSION     DBNGSB010000089.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2820780"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1467)
                     /locus_tag="AC159B_02535"
     CDS             complement(<1..1467)
                     /locus_tag="AC159B_02535"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR3376662.1"
                     /translation="MKLPAGTVTFLFTDIEGSTRLLQHLGGDRYTEVLSTYREILRGS
                     ATTFSGQEVDTSGDATFFAFSRVTDAVAAAVDAQHALDTHAWPEGTTVRSRMGIHTGQ
                     PLTSEGDYVGIDVHRAARICEAAHGGQIIVSDATRIVLEQTPPEAVTLKSMGEHRLKD
                     LLRPERLFQVTHPDLRENFPPLRTLDAHPHNLPPQPTPLIGREAELDQTRATLLDEGV
                     RLLTLTGPGGTGKTRLALQLAAHVIDRFHHGAFFVPLAHISDPEMVVPSVARALGVNE
                     TPGRPLLHNLQEYLSDKQTLLLLDNLEQLTQTSFVVDRLLSASSELKIIATSRERLRS
                     SWEHEFAVPMLGVPQDWKGDLDALSRNSAVALFVNRCRSVQRDFSLTPENAAAVATIC
                     ARLEGLPLAIELAAARIKILAPQEIATRLHDQLRLLKGGPRDAPERHQSLQAAFSWSY
                     ALLAPEEQRLFRRLAVFRGGFTLESAEAIGDHSELDVLD"
     gene            complement(1499..2302)
                     /locus_tag="AC159B_02540"
     CDS             complement(1499..2302)
                     /locus_tag="AC159B_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR3376663.1"
                     /translation="MTVVPAPAENEILFRKDGSLAFITFNRPHARNALTWQMYDGLSE
                     FCDRVDADAAVRVLILTGAGEQAFVAGTDIEQFRSFHTPQDATGYEQRIERVIDRLER
                     VGKPTIAMIRGACVGAGLAIAAACDFRICAPDLRLGAPIARTLGNCLSMNNYARVLDL
                     AGPARAKELIMLARLLSADEALRLGLVTEVVPADQLESRVRELAGELTQLAPLTLRTT
                     KEAIRRLQARRRLESGEGDDLIETCYMSDDFRGAVQAFLEKRPYVWIGR"
     gene            complement(2319..3533)
                     /locus_tag="AC159B_02545"
     CDS             complement(2319..3533)
                     /locus_tag="AC159B_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR3376664.1"
                     /translation="MTDETPRALQGIRVLDLSQVMAGPFCTMLLGDLGADIIKVEPPA
                     GDTTRQMAGATGDESPSFWAVNRNKRGIAVNLKDSRGVEIVRTLARTADVFVENFRPG
                     VAAGFGLDYGSLSTINPRLVYASISGFGQTGPYASRGGFDLVAQAMSGIMSVTGDTGL
                     PPMKCGLPITDLGAALFAVYGILAALLHRERSGRGQFIDTSLLDAGVAFSVWESAEYW
                     SGRGVPKPTGSAHRMSAPYQAIRCADGYIALGAANQRVWERLCQALGRPDLLQRQEFS
                     TDARRVEHRRELAGMIESVMRTKPSSHWLNALQRADVPCGPISDYEHVFSDPHVRARG
                     LVQEVAHPAAGRIKQLGPAVKFSATPAQISRPSPTFGQHTAEVLEELGYDRDAVRALA
                     ADGVVHVQRQAT"
BASE COUNT          680 a         1199 c         1242 g          686 t
ORIGIN      
        1 atcgagaacg tcaagctcac tatggtcacc gatggcctcc gccgattcca gtgtgaaccc
       61 acctcggaaa acggcgagcc gcctgaacag ccgctgctct tcgggcgcga gcaacgcata
      121 actccaagag aacgctgcct gcagcgactg gtggcgctcc ggtgcgtccc gcgggccgcc
      181 cttgagtaga cgcaattgat cgtgaagacg cgttgcgatc tcctgcggcg ccaggatctt
      241 gatgcgagcc gcggcgagct cgatggccag tggcaaccct tccagacggg cgcagatggt
      301 cgcgacggcc gccgcgtttt caggcgtcag cgagaaatcc cgctgcacag aacggcaccg
      361 gttcacgaac agggcgaccg cggagttccg cgacagagca tctaagtcgc ctttccagtc
      421 ctgcggcacc ccgagcatcg gcaccgcgaa ctcatgctcc caactcgagc gaagccgctc
      481 gcgactcgta gcgatgatct tgagctctga actcgcagag agcaggcggt cgacgacaaa
      541 ggacgtctgc gtgagctgct ccagattgtc gagcaggagc agggtctgct tgtcactgag
      601 atactcttgt agattgtgca ggagcgggcg gccgggagtc tcgttcactc ccagcgcccg
      661 tgcgacggaa gggacgacca tctccggatc gctgatgtgc gcgaggggca cgaaaaaggc
      721 gccgtggtgg aaccggtcga tcacgtgggc cgccagctgc agcgccagtc gcgtcttccc
      781 cgtgccccca gggccggtga gcgtgagcaa acgcacgcct tcgtcgagca gcgtcgcgcg
      841 ggtttgatcg agctctgctt ctctcccgat gagcggcgtc ggctgcggcg gcaggttgtg
      901 gggatgcgcg tccaacgtcc gcaggggagg aaaattttca cggagatcgg gatgcgtgac
      961 ctggaacagc cgctccgggc ggagcagatc cttcaggcga tgctcgccca tcgatttgag
     1021 cgtcacagct tccggcggcg tctgctccag cacgattcgg gtggcgtctg acacgatgat
     1081 ctgaccgccg tgcgcggcct cgcagatgcg ggccgcccgg tgcacatcaa tgcccacgta
     1141 gtcgccctcg ctggtgaggg gttggcccgt atggatgccc atccgcgacc ggaccgtcgt
     1201 gccttcgggc cacgcgtgcg tgtcgagcgc gtgctgggca tcgacggccg ctgcgaccgc
     1261 atctgtgact cgggaaaagg cgaaaaaggt agcgtcacct gacgtgtcga cttcctgtcc
     1321 gctgaatgtg gtcgccgaac cccgcagtat ttctcggtag gttgagagga cctcagtgta
     1381 gcgatcgccg ccgaggtgtt gaagaaggcg tgtcgatccc tcgatgtcgg taaaaaggaa
     1441 ggtgaccgtt ccagcgggca gtttcatcgt gcggcacctg atctggcgtt aatactttct
     1501 accgtcctat ccacacatag ggccgttttt caagaaacgc ctgcacggcc ccgcgaaagt
     1561 cgtcgctcat gtagcacgtc tcgatgagat catcgccttc gcctgactcc aggcgccgcc
     1621 tggcctgaag acgccgaatc gcttctttgg ttgtgcggag cgtgagcggc gccagctgcg
     1681 tgagctcgcc cgcgagttcg cgcacgcggg attccagctg atctgcgggg acgacctctg
     1741 tcacgaggcc caggcgcagc gcctcgtcgg cgctgagcag gcgcgccagc atgatgagtt
     1801 ccttagcgcg ggccggtccc gccagatcga gcacgcgggc atagttgttc atcgaaaggc
     1861 agttgcccaa cgtgcgggcg atcggcgcgc ccaggcggag gtccggcgcg cagatgcgaa
     1921 aatcgcaggc cgccgcgatc gcgagtccgg ccccgacgca tgcgccgcgg atcatggcga
     1981 tggtcggctt gccgacacgt tccaaccgat cgatgacgcg ttcgatccgc tgctcgtacc
     2041 cggtcgcatc ctgaggcgtg tggaacgacc ggaactgctc aatgtccgtg ccggcgacga
     2101 acgcctgctc tccggcgccg gtcagaatga gcacacgaac cgcggcatcc gcgtcgacgc
     2161 ggtcgcagaa ctcggacagc ccgtcgtaca tttgccaggt cagcgcattg cgcgcatgcg
     2221 gccggttgaa cgtgatgaac gcaaggctgc catcttttcg aaagaggatc tcgttctctg
     2281 cgggtgcggg caccacggtc acctcattct cttggtggtc atgtcgcctg tctctggaca
     2341 tgtacgacgc cgtcggccgc caaagcgcgg accgcgtcgc gatcgtaacc gagttcctcg
     2401 aggacctccg cggtgtgctg tccgaaggtg ggcgagggac gactgatctg tgcgggtgtc
     2461 gccgagaact tcacggcggg gccgagctgt ttgatgcgac cggcggcagg gtgcgcgacc
     2521 tcctgcacca gtccccgagc gcgcacgtgc ggatcggaaa acacgtgctc ataatctgaa
     2581 atggggccgc acggtacatc ggcgcgctgc agagcgttaa gccagtggga ggacggtttc
     2641 gttcgcatga cgctttcgat catgccggcg agttcccggc gatgctcgac ccgccttgcg
     2701 tccgtcgaaa attcttgacg ctgtaagaga tccgggcggc cgagggcctg gcagagccgc
     2761 tcccacacgc gctgattcgc cgcgccgagc gcaatatacc cgtcggcaca gcggatcgcc
     2821 tgatagggtg cggacattct gtgcgccgac cccgtcggct ttgggacgcc gcggcctgac
     2881 caatactcag ccgattccca caccgagaag gccacccccg catcaagcag tgaggtgtcg
     2941 atgaattgtc cgcgcccgct tcgctctcga tggagaagcg ccgcgaggat accgtagacg
     3001 gcgaagagcg ccgcacccaa atcggtgatg gggagtccac atttcatggg cggcaggccc
     3061 gtatcgccgg tgaccgacat gattccggac attgcctgcg cgacgagatc gaaccctccg
     3121 cgtgacgcat acgggccggt ctgcccaaat cccgagatcg acgcgtaaac gaggcgcggg
     3181 ttgatcgtcg acagcgagcc ataatcaagt ccgaagcccg cggcgacgcc gggccggaag
     3241 ttctcgacga acacgtccgc cgtgcgcgcg agagtgcgga cgatctccac cccgcgagaa
     3301 tccttgaggt tgacggcgat cccgcgcttg ttccggttga cggcccaaaa gctggggctt
     3361 tcgtcgcctg tggctccggc catctgtcgg gtcgtatcgc cggcgggcgg ctcgactttg
     3421 atgatgtcgg cgcccagatc tccgagcagc atcgtgcaga acggcccggc catgacctga
     3481 ctgagatcga ggacacggat cccttgcagt gcgcgcggtg tttcgtcggt catcacaaag
     3541 ctcacctctc gcgcgacttg gcttcctgcg ttgtgacttc ctgcacatgc catggagagg
     3601 aacgcgaagt cggcgctgcg aacaccaccc ctgaagcaga gtggggggtg caattgtgga
     3661 catgcgacgc atcatgacgg tcgtgcttgc ggcgatcgta gttatcgtcg cgccgacgct
     3721 gtacacgatc ccgcaggccg gcgcgcagca gaggatcatc gtccggctcg gccatgtcgg
     3781 ctttccggac tcattgtttg agattac
//
LOCUS       DBNGSB010000090         2963 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_521754, whole genome shotgun sequence.
ACCESSION   DBNGSB010000090 DBNGSB010000000
VERSION     DBNGSB010000090.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_521754"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="AC159B_02550"
     CDS             <1..291
                     /locus_tag="AC159B_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376665.1"
                     /translation="QAQTGPGGSAPDPAKAKGEVTVGRLADATIDGAKTHGYQYTTRS
                     DGPPVQSRVFVLADRSLPRRTEVLDEGGKVQSTIDYYDYNVPITIDLPKCAN"
     gene            complement(336..1589)
                     /locus_tag="AC159B_02555"
     CDS             complement(336..1589)
                     /locus_tag="AC159B_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3376666.1"
                     /translation="MSAPLITAQQTRSPSDILIRNARLRASPDPIDIAIHSGRIAAIS
                     TAISSDAKTEIDAQGRLVTESFVNPHIHLDKVYTLGMLGDEALRAYHGGSMENAAAAI
                     ELASRVKARYQQSWIVENVRKAIKEAVRHGTTHMLALADVDTKARLEGVNALIQAREE
                     FRGLVEIRVVAFPQDGVVREPGAAALVHQAIEMGADVVGGIPWIEHTEADMRRHIDEM
                     FRIAAKFDKPISMLVDDAGRPELHTLEMLAEKTIEEQWHGRTLAQHARATSLYKEERL
                     RKVAALLREARMAIVTDPHTGPLHVPVRALLDEGVLLCLGQDDISDAYYPFGRNNMLE
                     VAFLASHLLWMTTATDMEKLYDMITVDAARAMWMQDHRLEVGAPANLVVLNAPNVLEA
                     FRSHEPPAYVVSNGKVVAEAGSLRA"
     gene            1601..>2963
                     /locus_tag="AC159B_02560"
     CDS             1601..>2963
                     /locus_tag="AC159B_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3376667.1"
                     /translation="MLPTGTITFLFTDIEGSTRLWEQDPDEMRRALTVHESILREEIA
                     RQSGQIFKTVGDGVYAAFGDAPSAIAAARDAQQRLMATSWGVPGGVRVRMALHTGTAQ
                     LQDGDYFGPALNRIARILSAAHGGQILISESTRTLVERDLPEGASLRDQGLHRLKDLA
                     QPEHIFQFVTPELPDAFPQLRTLDAFPNNLPRQLTSFVGREREMKEVKELISAAALLT
                     LTGSGGSGKTRLALQAGADLVERFKDGVWLVELDVLSEESMVLRAIASAVGVHEELSF
                     VARPLGNVLVEALREKSLLLILDNCEHVLGPSADAASLLLRTCPDVHILATSQERLGI
                     AGERVYQVQSLSTPGRQQPPAPERLAEFEAVRLFVERAILSHPHFTLTEANAPAVADV
                     VQQLEGIPLAIELAAARVKVMSVDQIGARLNERFRLLTAGPRTETPRHQTLRAALDWS
                     YDLL"
BASE COUNT          548 a          932 c          908 g          575 t
ORIGIN      
        1 caggctcaga ctgggccggg tggatcggca cctgacccgg caaaggccaa gggcgaggtg
       61 acggtgggcc ggttggccga cgcaacgatt gatggggcga aaacgcacgg gtatcaatac
      121 acgacgaggt ctgacggccc gccggtgcag agccgcgtct tcgtcctggc ggaccgcagt
      181 ctgccgcggc ggacggaggt cctcgacgag ggcggcaagg tgcagtcgac gatcgactac
      241 tacgactaca atgttccgat cacgatcgac ctgccgaagt gcgctaatta gtcattgcga
      301 ggcccgaagg gccgaagcaa tccgagattg cttcgctacg ctcgcaatga ccctgcttcg
      361 gccacaacct tgccgttact cacaacgtac gcggggggct cgtgcgaccg aaacgcctcc
      421 agcacgttgg gcgcattcag caccacgagg ttggccggcg cgccgacctc gaggcggtga
      481 tcctgcatcc acattgccct ggccgcgtcg accgtaatca tgtcatagag tttctccatg
      541 tcggtcgccg tcgtcatcca cagcaggtgg ctcgccagga acgcgacctc gagcatatta
      601 ttccggccga acggatagta ggcatccgag atatcgtcct gccccaggca caggagcacg
      661 ccttcatcta agagggctct cacgggtacg tgcagcgggc ccgtgtgagg atcggtgacg
      721 atggccatgc gggcctcgcg gagcagcgct gcgactttcc ggagacgttc ttccttatat
      781 aacgacgtcg cacgggcatg ctgagccagc gtgcggccgt gccactgttc ttcgatcgtc
      841 ttctccgcca gcatttcgag cgtgtgcagc tcgggtcggc cggcgtcatc gaccagcatc
      901 gagattggct tgtcgaactt cgcggcaatt ctaaacatct cgtcgatgtg ccgccgcatg
      961 tccgcttcag tatgttcgat ccacggaatc ccgcccacga catcggcgcc catctctatc
     1021 gcctgatgga ccagcgccgc tgctcccggt tccctgacaa cgccatcctg cgggaacgcg
     1081 acgacccgga tctcgaccaa gccgcgaaat tcttcgcgag cctggatgag cgcgttcaca
     1141 ccttcgagcc tcgccttcgt gtccacgtcg gccagtgcca gcatatgtgt ggtgccgtgc
     1201 cggaccgctt ccttgattgc tttgcgaaca ttttcgacga tccaggactg ctgataacgc
     1261 gccttgaccc gtgacgcgag ttcgatggct gcggccgcgt tctccatcga cccgccgtga
     1321 tacgcgcgca gcgcttcatc gcccagcatg cccagcgtat agaccttatc gaggtggata
     1381 tggggattca caaacgattc cgtcacgagg cgcccttgcg catcgatctc tgtcttcgca
     1441 tcactagaaa tggcggtcga gatcgcagcg atccggcccg agtgaatcgc gatgtcgatg
     1501 gggtccggcg aggctcgcag ccgtgcgttt cgaatcagga tgtccgaagg agaacgtgtt
     1561 tgctgcgccg taatcaaggg ggcgctcatc ggaggtacct atgctcccca ccggaacaat
     1621 cacctttctc ttcacagaca tcgaggggag caccaggctc tgggagcagg accctgatga
     1681 gatgcgcagg gcgctgaccg ttcacgaatc aatcctgcgc gaggagatcg cacgccaaag
     1741 tggacagatc ttcaagaccg tcggtgacgg tgtctacgcc gcttttgggg atgcgccgag
     1801 cgccatagcg gccgccaggg atgcgcagca gcgcctcatg gccacttcct ggggagtacc
     1861 gggcggcgtg cgcgtgcgta tggcgctgca cacaggtacc gctcaactgc aggatgggga
     1921 ctacttcgga ccggcgctca atcggatcgc caggatcctg tcggccgctc acggcggaca
     1981 gatcctcatc tccgaatcga ctcggacgct ggtcgagcgt gacctgccag aaggcgccag
     2041 cctgcgcgac caggggctgc accggttaaa ggatctggcg cagcccgagc acatcttcca
     2101 attcgtgact ccggaattgc cggacgcctt ccctcaactt cggacgctcg atgcctttcc
     2161 aaacaacctc ccgcgccagt tgacgagttt cgtgggtcga gagcgagaga tgaaggaggt
     2221 caaagagctc atctcagcag ccgccctcct cacgctcaca ggatcaggtg gatcaggaaa
     2281 aacacggctc gcattgcagg ccggcgccga tcttgtggaa agattcaagg acggcgtctg
     2341 gctggttgag ctggatgtcc tctccgagga gtcgatggtc cttcgcgcca ttgcgtccgc
     2401 ggtcggcgta catgaggagc tgtcgttcgt cgcgcggcct cttggcaatg tcttggtcga
     2461 ggccctccgt gaaaagtcgc tgctgctcat tctcgacaac tgcgagcatg tactgggtcc
     2521 gtcggcagac gcggcatcgt tattactgcg gacgtgcccg gatgtgcaca ttctcgcgac
     2581 gagtcaggaa cggttgggca tcgcgggaga acgcgtgtac caggtgcaat cgctctcaac
     2641 ccccggtcgt cagcagcctc ccgctcctga gcgtctcgcg gagtttgaag ccgtccgcct
     2701 gttcgttgag cgcgccatcc tgagccatcc gcactttacg cttaccgaag cgaacgcgcc
     2761 ggcggtggct gacgtcgttc agcaactcga aggcattccc ttagccatcg agctggccgc
     2821 tgcgcgggtc aaggtcatgt cggtcgatca gattggcgct cgcttgaacg agcgcttccg
     2881 gctgctcacg gccggtcctc gaacggagac gccgcgccat caaacactgc gcgccgcgct
     2941 ggactggagc tacgatctct tgt
//
LOCUS       DBNGSB010000091         2487 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_522286, whole genome shotgun sequence.
ACCESSION   DBNGSB010000091 DBNGSB010000000
VERSION     DBNGSB010000091.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_522286"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /gene="frdD"
                     /locus_tag="AC159B_02565"
     CDS             complement(<1..343)
                     /gene="frdD"
                     /locus_tag="AC159B_02565"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF003977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HIR3376668.1"
                     /translation="MKRSNEPIWWSLFAAGGVVAALFIPVLILLTGIAGPLGWTAQAF
                     AYDRIAALAGHPLSRLFLFVVISLPLFHWAHRFRFTLVDLGLKGARNAIASACYGAAI
                     LGTAVAAFVLIT"
     gene            complement(340..720)
                     /locus_tag="AC159B_02570"
     CDS             complement(340..720)
                     /locus_tag="AC159B_02570"
                     /inference="COORDINATES: protein motif:HMM:NF014366.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit C"
                     /protein_id="HIR3376669.1"
                     /translation="MSRPYHRPMPANWWLTNRAYFMFMIRELTSVFIAIYVVLILVLI
                     NRIAAGPDAYRAYLAFLATPGMLVFHIVALAAALFHSITWFNLAPTGLAVRVGERRVP
                     DAVIVGSNFAAWIILSVLLAWIVL"
     gene            complement(717..1463)
                     /locus_tag="AC159B_02575"
     CDS             complement(717..1463)
                     /locus_tag="AC159B_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HIR3376670.1"
                     /translation="MVDQTITLEIFRYRAEQESGPTFQCYDVPYRDDWVVLDAINYIK
                     DRLDGTLSYRWSCRMGVCGSCGMMINGVATLSCAAFLKDYFPHPIRVEPLANFPVQRD
                     LVIVMDDFIEKLGHVKPWLIREDAPPLSQGELRQTPAQLEAFKQYSMCINCMLCYAAC
                     PVYGLEPRFLGPAAIALAQRYNLDSRDRGNAERQAALTSEDGIWDCTLVGECTVVCPA
                     HVDPAGAIQRAKVTATVDYYKDVLLPWGKR"
     gene            complement(1490..>2487)
                     /locus_tag="AC159B_02580"
     CDS             complement(1490..>2487)
                     /locus_tag="AC159B_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR3376671.1"
                     /translation="GEGGIMVNKDGYRYLQDYGMGPPDPWPRLRGMELGPRDRLSQAF
                     VHEMRKGRTIKGPYGDIVYLDIRHLGEEKIDKKIPFVRELARRYAGIDPVHEPIPVRP
                     VVHYMMGGVHTDLHGATPLRGLYAAGEVACVSINGANRLGSNSLTECLVFGARAGKSA
                     AAFALEHKRVNRGALEARARDETERLSREFFRKDSGTERVAALRHEMTTAMETGAGIY
                     RDEGQLRETCDQLAALRERFTRVRLDDRSRGFNTELIAALELDCMLDVASAIGHSALA
                     RTESRGSHQRTDHPERDDARFLKHTMAYRTEEEPRIEYHDVVITRWPAGERVYGR"
BASE COUNT          490 a          789 c          773 g          435 t
ORIGIN      
        1 gtgtgatcag gacgaaggcc gcaaccgccg ttcctaggat cgccgcgcca tagcaggcac
       61 tcgcaatcgc atttcgcgca cccttcaacc caagatcgac caatgtgaag cggaaccggt
      121 gcgcccagtg gaagagcggc agggaaatga cgacgaagag gaacaggcgg ctcagggggt
      181 gaccggccaa cgccgcgatc cgatcataag caaacgcctg tgcagtccat cccaaaggcc
      241 cggcgatgcc cgtgagaagg atcaggaccg ggatgaacaa cgcggcaacc acgccgccgg
      301 ccgcgaacag agaccaccag atgggttcgt tggaacgctt cataggacga tccaggcgag
      361 aagcacggac aggatgatcc atgccgcgaa gttgctcccg acgatgaccg cgtctgggac
      421 ccgccgctcg cccacccgga ctgcaagccc cgttggcgcc agattgaacc aggtgatcga
      481 atgaaagagg gcggcggcca gcgcgacgat gtggaacacc agcataccgg gcgtcgccag
      541 gaaggcgaga taggcgcgat acgcgtcagg tccggctgcg atcctgttga tcaagacgag
      601 tatcagcacc acatagatgg cgatgaagac gctcgtcagt tcgcggatca tgaacatgaa
      661 gtaagcgcga ttggtcagcc accagttcgc cggcatggga cgatggtacg ggcggctcaa
      721 cgcttccccc acggcagcaa cacgtctttg taataatcca cggtggccgt gaccttcgcc
      781 cgctgaatgg cgccggcggg atccacgtgc gccgggcaga ccaccgtgca ctcgccgacc
      841 agcgtacagt cccagatacc gtcctcgctc gtgagcgccg cttgacgttc tgcgttgccc
      901 cggtcgcgcg aatccagatt gtaccgttgc gccagcgcaa tcgccgccgg tccgagaaag
      961 cgcggttcca gaccgtagac agggcaggcg gcgtagcaga gcatgcagtt gatgcacatg
     1021 ctgtactgtt taaaggcttc gagttgggct ggagtctggc gtagctcacc ctgtgaaagt
     1081 ggcggcgcat cctcacggat gagccacggc ttgacgtggc cgagcttctc gatgaagtca
     1141 tccatgacga tgacgaggtc gcgctgcacg gggaaattcg ccagcggctc gacgcggatg
     1201 ggatggggga agtagtcctt gaggaatgcg gcgcagctca aggttgccac accgttgatc
     1261 atcatgccgc agctgccaca cacgcccata cggcacgacc agcggtagga tagggtcccg
     1321 tcgagacggt ctttgatgta gttgatcgca tccagcacca cccagtcgtc ccggtaaggc
     1381 acgtcgtagc actggaatgt gggtccactc tcttgctccg cgcgataccg aaaaatctcc
     1441 agcgtgatcg tctggtcaac catccatgtc ctccggccgt ccgatctctc tatcgtccat
     1501 agacgcgctc gcctgccggc caccgcgtga tcacgacatc gtggtactcg atccgcggct
     1561 cctcctcggt gcgatacgcc atcgtgtgct tgagaaaccg cgcatcgtcc cgttccggat
     1621 gatcggtgcg ctggtgcgag ccgcgcgatt cggtgcgggc cagcgcggag tgtccgatcg
     1681 cgctcgcgac gtcgagcata cagtcgagct cgagggccgc aatgagttca gtgttgaacc
     1741 cccggctgcg atcgtccaga cggacacggg tgaaccgctc ccgcaatgcc gccagctggt
     1801 cgcacgtctc gcgcagctgg ccttcatcac gatagatccc cgcaccggtt tccatagcgg
     1861 tggtcatctc gtggcgcagg gctgccaccc gctcggttcc agaatccttg cggaaaaact
     1921 cacgcgagag acgctccgtc tcgtcgcggg cgcgggcttc gagcgcgcca cgattgaccc
     1981 gtttgtgctc gagggcgaat gcggcggcgc tcttgcccgc ccgcgcgcca aagacgaggc
     2041 actccgtcag tgagtttgac cccaggcggt tcgccccgtt gatactgacg catgcgacct
     2101 cgcccgcggc gtacagcccc cggagggggg tggcgccgtg caggtcggtg tgcacaccgc
     2161 ccatcatgta atggacgacc ggtcggaccg gaattggctc gtgaaccgga tcgatgcccg
     2221 cgtagcgccg ggccagctca cggacgaatg ggatcttctt gtcgattttt tcctcaccca
     2281 gatggcgaat gtcgagatag acaatatcgc catacggtcc cttgatcgtg cggcccttgc
     2341 gcatttcatg cacaaacgct tgagaaagcc tgtcgcgggg gccgagttcc attccccgca
     2401 gccgcggcca cgggtccggc ggtcccatgc cgtagtcctg gagatagcga tagccatcct
     2461 tattgaccat aatgccgcct tcgcccc
//
LOCUS       DBNGSB010000092         2122 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2822156, whole genome shotgun sequence.
ACCESSION   DBNGSB010000092 DBNGSB010000000
VERSION     DBNGSB010000092.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2822156"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="AC159B_02585"
     CDS             <1..219
                     /locus_tag="AC159B_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3376672.1"
                     /translation="VAVVAPSERAAAAGFTNVVRNLAGAVTPIISGYAMQVVSLGLPF
                     VIGGGLKIVYDLTLFAMFRSVPPREETS"
     gene            216..944
                     /locus_tag="AC159B_02590"
     CDS             216..944
                     /locus_tag="AC159B_02590"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3376673.1"
                     /translation="MIEGVIFDLGGTLIERGRRSFEPGNATALTEWLRRGDHHVGAEF
                     ADAVVAEREASFARRTELREAQAGEALRPVFGRYGMPTDDAFVTAAEVAFYEPELRAM
                     RLLPGTVELLQQLHARQLRIGLASNATSHYFVQECCKRLGISRYLDPIMSSAAVGWAK
                     PDARIFKAILQSWAMTPGHAVMVGDSVGADIAGARTMGMRSILLTAEHDQGEPRTWEG
                     VQADAEAATLKETGRIIERWLQES"
     gene            complement(941..>2122)
                     /locus_tag="AC159B_02595"
     CDS             complement(941..>2122)
                     /locus_tag="AC159B_02595"
                     /inference="COORDINATES: protein motif:HMM:NF013237.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-sulfuration enzyme family protein"
                     /protein_id="HIR3376674.1"
                     /translation="RVMEKKWGFSTKAIHGGKISDAHKSVAVPIYQTATYYYDTAEEG
                     ARLGQEIPPGFVYSRWSNPTTRALEEKIALLEDAEDALATASGMGAVSCAVLTALKRG
                     DHAIAPSAIYQATYQLFAEMLPSYGIETTLLPDTDTQSYERALRPNTRLLFIETPNNP
                     MLAITDAVKVASLARAHGAMTIADNTWATPYNHTPIRDGVEAVVHSATKYLGGHHDVT
                     LGVIAGSSDFIKKAKKHVRILGTTPDPFGAWLVIRGLSTLALRVERQNTTALRLSEFL
                     AEHPKVDRVHYPGLGAHPGHAVAARQMRGFGGMLSFEVKGGYDPSVRVFESLEIPKRA
                     TSLGGVSTLVSHPAGISSVHMPKEVRAAAGISEGLIRVSVGVEDVDDLLGDFSQALDR
                     V"
BASE COUNT          398 a          646 c          671 g          407 t
ORIGIN      
        1 gtggcagtcg tggcgccgtc agagcgggcg gcggcggcgg gattcaccaa cgtagtccgg
       61 aatctagcgg gggccgtcac gcctatcatt tctggatacg cgatgcaggt ggtctcactc
      121 gggcttccgt tcgtgatcgg cggcggcctg aagatcgttt acgacctgac actgttcgcg
      181 atgtttcggt cggtcccacc acgagaggag acctcgtgat cgaaggggtc atcttcgatc
      241 ttggcggcac cctaatcgag cgcggccgca ggagcttcga gcctggcaat gcgacggcgc
      301 tcacagagtg gctgcgtaga ggggaccacc acgtcggtgc ggaattcgcg gacgccgtcg
      361 tcgccgaacg ggaagcgtca tttgcccggc gcacagaact gcgcgaggcc caagcggggg
      421 aagccctccg gcccgttttt ggacggtacg ggatgccgac cgacgacgcg tttgtaactg
      481 cggccgaggt ggcgttctat gagccagaac tacgcgcaat gcgtctgctg cccggcactg
      541 tggaactgct gcaacagctc cacgcgcgtc aattgcgcat cggcctggca tcaaacgcca
      601 cgtcacacta tttcgtgcag gaatgctgca aacggcttgg gatcagccgc tatcttgacc
      661 cgatcatgtc atccgccgcc gttgggtggg caaaaccgga cgcgcggatc ttcaaggcaa
      721 tcctgcagtc gtgggcgatg accccgggcc acgcggtcat ggtcggcgac tccgtcggcg
      781 cggacattgc cggcgcacga accatgggga tgcgatcgat cctgctgacc gcggagcacg
      841 atcaaggcga accccgaaca tgggaaggag tgcaggcgga cgctgaggcg gcgaccctca
      901 aagaaaccgg ccggataatc gagcgttggc ttcaggaaag ctaaacgcgg tctagggcct
      961 gcgagaaatc tccgagcaaa tcatccacgt cctcaacacc cacgctcacg cgaatgagac
     1021 cttcactgat cccagcagcg gcccggacct ccttcggcat gtggacactg ctaataccag
     1081 ccggatggga gaccagggtg ctgacgccac cgaggctcgt agcacgcttg gggatctcaa
     1141 ggctctcaaa gacgcggacg ctcgggtcgt agccgccttt cacttcgaaa ctaagcatcc
     1201 ccccaaaccc gcgcatttgc ctggctgcga ccgcgtgccc gggatgggcg cccagtcccg
     1261 gatagtgcac gcgatccact ttaggatgct ccgcgaggaa ctctgacagg cgcagggccg
     1321 tcgtgttttg gcgctctacg cgcagcgcca acgtggagag tcctcggatc accagccacg
     1381 cgccgaaggg atcgggtgtc gttccaagga tcctgacgtg ctttttggct ttcttgatga
     1441 aatcggacga cccggcgatg accccgagcg taacgtcgtg gtggcctccg agatacttgg
     1501 ttgcgctatg cacgaccgcc tcgacgccat cacgaatggg cgtatggttg tatggtgtgg
     1561 cccacgtgtt gtcggcgatc gtcatcgcgc cgtgcgcgcg ggcaagcgag gcgaccttga
     1621 ccgcatcagt aatcgctagc attgggttgt tcggagtttc aatgaagagc agccgggtgt
     1681 tgggacgcag cgcgcgctcg tacgattgtg tgtccgtatc cggcagcagg gttgtttcga
     1741 ttccgtagga tggcaacatc tctgcgaaga gctgataggt cgcctgatag atcgcgctgg
     1801 gtgcgatcgc atgatcgcct cgcttgagcg ccgtcaatac cgcacacgag acagcaccca
     1861 ttcctgaggc cgtcgccaac gcgtcttctg cgtcttcgag cagggcgatc ttctcttcca
     1921 gcgctcgcgt cgtggggttg ctccagcgcg agtacacaaa gccgggcggg atctcttggc
     1981 ccagccgcgc gccctcctcc gcagtgtcgt aatagtaggt cgccgtctga tagatgggaa
     2041 ccgcgaccga cttatgagcg tcagaaatct tgccgccgtg aatcgccttc gttgagaagc
     2101 cccacttctt ttccattacc cg
//
LOCUS       DBNGSB010000093         4185 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_62104, whole genome shotgun sequence.
ACCESSION   DBNGSB010000093 DBNGSB010000000
VERSION     DBNGSB010000093.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4185)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_62104"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="AC159B_02600"
     CDS             complement(<1..818)
                     /locus_tag="AC159B_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3376675.1"
                     /translation="MAGIQERGAAADLRLGRAHARPRRVTLYPYLSILPALLVVALIS
                     VYPLLFALDLSVHRNILTDPLTHPFVGLHNFAETFGSYYFVRALLTTLRFTAMIIAGT
                     LVYGIAVSLFLNRPGRLAGASRIAILLPWAIPTVMAAIIWRWIFNGNYGALNALLVGI
                     GVLPEYISWLGNPLLAQLSLVLTHIWKWGPLAAIMCLATLQVIPRDLYEAAYIDGGGG
                     WAVFRFVTLPFLRPTILLLLILETIAGFVTFDLIYVLTGGGPADATTMLAWFAYA"
     gene            complement(823..2076)
                     /locus_tag="AC159B_02605"
     CDS             complement(823..2076)
                     /locus_tag="AC159B_02605"
                     /inference="COORDINATES: protein motif:HMM:NF013697.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3376676.1"
                     /translation="MLPAAHAAPQVTLNFMVWTFAVDTIQENIKRFHARYPNINVRLT
                     DYNWGQFPDTIVANFVGGSNAPDVLYSSDHWLQQWAAAGWIAPLDDYFPQVKEMAKSF
                     SQYAVEGMSYKGKIYGLPYYADPMAFFYNEAIVKKAGFTLPPSTWEELLQQAKVIKEK
                     GLVKYPIGFGFSQQEPFSIEVFISMVYSRGGNLFDKDMNPVFDRSGSATIKSIEWIRD
                     ALRAGVLDPESLQWDGVQAGNAMRAGNQVYSISRASALWSFNNPQQSKEAGNFKIALM
                     PGSTHQTVGFVRFYAMSSQVPKRGKEALDAAWNFLNYFGGPGPDNSYPVVRRWALDFG
                     LGFAQMPLFRDTDILLSFGKWVDVPLLQRIGQRYSKVKQGLTPWFAQWDVFTRAELQK
                     AYLGQQSPDQTAKNMAAKWNELKRK"
     gene            complement(2194..3003)
                     /locus_tag="AC159B_02610"
     CDS             complement(2194..3003)
                     /locus_tag="AC159B_02610"
                     /inference="COORDINATES: protein motif:HMM:NF013757.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR3376677.1"
                     /translation="MRGGGLPRNLDRYAVHVVDRALDVLETLRDAGRPLGLQDVATRL
                     RTPKSSVFRLLCTLERRGYVDRTPDSLYRLGAHWLSYRDLAGKHPPLTEIAVPYMRRL
                     LDAYGETVNLGVLREGKIVYLEMLESAHSFRMAAAAGTISPVHSTALGKAIAAFLPEA
                     VIDATIRTRGLPALTPRTITSRAAWKRELARTRVRGYAEDNGETEPEVTCIGAPIFGV
                     DGNVIGAISVSGPASRMRSIRQRVARALVDACTAISRALGPASERRAQRGG"
     gene            complement(3140..3781)
                     /locus_tag="AC159B_02615"
     CDS             complement(3140..3781)
                     /locus_tag="AC159B_02615"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HIR3376678.1"
                     /translation="MVWELVAPQLRQYLDALVPPRPAEMQAMEAFARETRFPIVGPAA
                     GNLCYLLARLTRARRVFEMGSGFGYSTAWFARAVQENGGGTVFHVVWDEELSRRARKH
                     LGALGFEDVVQYHVGEAVETLSRAEGPFDVIFNDIDKEAYPQSLSVIAKKLRPGGVLI
                     VDNMFWQGRIFDEADRSATTQGVRELTRQIFSDPRWISSVVPIRDGLLVAYQL"
     gene            complement(3860..4093)
                     /locus_tag="AC159B_02620"
     CDS             complement(3860..4093)
                     /locus_tag="AC159B_02620"
                     /inference="COORDINATES: protein motif:HMM:NF040444.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HIR3376679.1"
                     /translation="MARYGRSAQKHVESAMRRRKRGTLRSGKRGPGKGKKVTSPEQAI
                     AIGLSEARRKGGKVPPPPGRRHSRSRKTRARRS"
BASE COUNT          826 a         1273 c         1308 g          778 t
ORIGIN      
        1 gcgtacgcaa accaggccag catagtggtg gcgtcggcag gaccgccccc cgtgagcaca
       61 tagatgaggt cgaacgtgac gaagcccgcg atcgtctcga ggatgagcag aaggaggatg
      121 gtaggtcgga ggaaggggag cgtgacgaat cggaacaccg cccagcctcc accaccatcg
      181 atgtacgctg cttcgtagag atcccgaggg atgacctgca gcgtcgcgag gcacatgatc
      241 gcggcgagcg gtccccactt ccagatgtgc gtgaggacca gtgacaactg cgcgagaaga
      301 gggttgccga gccaggagat gtattctggg agcacgccga tgcccacaag gagcgcattg
      361 agcgctccat agtttccgtt gaagatccag cgccagatga tcgccgccat gacggtggga
      421 atggcccacg gcagaaggat ggcgatgcgt gaggcaccgg caagacgacc ggggcggttc
      481 aggaacaggc tgaccgcaat cccgtagacg agggtgccgg cgatgatcat cgctgtgaat
      541 cgcagcgtcg tgagcagggc acggacgaag tagtagctgc cgaacgtctc tgcgaaattg
      601 tgcaggccca cgaaggggtg cgtgagcggg tcggtcagga tgttgcgatg cacgctcagg
      661 tcgagcgcga agagtaaggg atagacggag atcaacgcaa cgacgagcag cgccgggagg
      721 atcgaaagat aggggtaaag cgttacgcgt ctgggccggg cgtgagcccg gcccagacgg
      781 agatccgccg ccgcgccacg ttcctggatg cccgccaacg ccctatttcc gtttcagctc
      841 attccacttg gcggccatgt tcttggcggt ctgatctgga gattgctggc cgaggtaggc
      901 cttctgcagt tccgcccgcg tgaacacgtc ccactgggcg aaccaggggg tgagaccctg
      961 cttcaccttt gaatagcgct gcccgatccg ctggagcagc gggacatcta cccacttacc
     1021 gaagctgagc aggatgtcgg tgtccctgaa cagcggcatc tgtgcaaagc ccagcccgaa
     1081 gtcgagcgcc cagcggcgta cgaccggata gctgttgtct ggaccgggtc cgccgaagta
     1141 gttcaggaag ttccacgccg cgtccagagc ctctttaccg cgcttcggta cctggctcga
     1201 catggcatag aaacgtacga acccgaccgt ctgatgggtg gagcccggca ttagcgcaat
     1261 cttgaagttc cccgcctcct ttgactgttg cgggttgttg aacgaccaga gtgctgatgc
     1321 ccggctgatg ctgtagacct gattgcctgc ccgcatagcg ttcccggcct gaacaccatc
     1381 ccactggagg gattcgggat ccaacacgcc ggcgcggagg gcgtcgcgaa tccactcgat
     1441 cgatttgatg gtcgcactcc ccgaacgatc gaacacaggg ttcatgtcct tgtcaaacag
     1501 attgccgccc ctggagtaga ccatggaaat aaacacctcg atggagaacg gctcctgctg
     1561 ggagaagccg aatccgatcg gatacttcac gaggcccttt tctttgatca ccttggcctg
     1621 ctggagcaac tcttcccagg tcgacggagg gagggtgaac cccgccttct tcacgattgc
     1681 ctcgttgtaa aagaaggcca tggggtccgc gtagtagggc aagccgtaga tcttgccctt
     1741 gtagctcatc ccctcgaccg cgtactgcga aaaagatttg gccatctcct tgacctgcgg
     1801 gaaatagtcg tccagggggg cgatccaccc tgccgccgcc cactgctgca gccagtgatc
     1861 cgagctgtag agcacgtcag gcgcattcga gccgccgacg aagttggcta cgatggtgtc
     1921 gggaaactgg ccccagttgt agtcggtgag acggacgttg atgttgggat agcgtgcatg
     1981 gaagcgcttg atgttttcct ggatcgtatc cacggcgaag gtccagacca tgaagttgag
     2041 cgtcacctgg ggcgcggcat gagccgccgg aagcacgggg aacaggctca ccgtcaggac
     2101 gaggacggcg agccagatca gcagtcttcc cttcattggt tttgcctcct caattgactg
     2161 aaccacggta ctgcatcact cagcgcgcgt ttctcacccc cctcgctgcg cgcgccgctc
     2221 ggatgccggc cccaacgcgc gtgaaatcgc cgtacatgcg tccaccagcg cccgggccac
     2281 gcgttgcctg atcgatcgca ttcggcttgc aggtccagac acgctgatgg ctccgatcac
     2341 attgccgtcc acaccaaaga tgggagcacc gatgcatgtc acttcgggct cggtctctcc
     2401 gttgtcctcc gcgtagcctc gcacgcgcgt gcgcgcgagc tcacgcttcc acgccgcccg
     2461 ggaggtgatg gtgcgaggtg tgagcgccgg cagcccacgc gtgcggatgg tagcgtcgat
     2521 cacagcctca ggaagaaacg cagcgatggc tttaccgagg gcagtcgagt gcaccgggga
     2581 aatggtgccg gccgctgccg ccatcctgaa cgaatgcgcg ctctccagca tctcaaggta
     2641 gacgatcttc ccctcccgca gcacacccag gttcactgtc tctccatagg cgtccaagag
     2701 ccggcgcatg taggggaccg cgatttccgt gagaggaggg tgtttcccgg cgagatcccg
     2761 gtaactcagc cagtgcgcgc cgagccgata gaggctgtct ggggttcgat ccacatatcc
     2821 cctgcgttcg agcgtacaga ggagccggaa tacggagctt ttgggagttc gcagccgtgt
     2881 agcaacgtcc tggagcccga gcgggcggcc cgcgtcccgg agtgtctcca gaacgtcgag
     2941 cgcgcgatcc actacgtgga ccgcatatcg gtcaaggttg cgcggaaggc ccccgcctcg
     3001 catatttcag tgaatgaaac cggcgttttg ggtcttaagc cgaccgtact cggggcgctg
     3061 cgtgttgtca agactgaaat gccgcccccc gtcgggcgcg agtcagagga gccgctaact
     3121 gccggacgtc tgaagaaatc tagagttggt aggctaccag cagcccgtcg cgaatcggca
     3181 cgaccgatga gatccatcga ggatccgaga agatctgccg ggtcaattcc cgcacgcctt
     3241 gggtggttgc cgagcgatca gcctcgtcga agatgcgtcc ttgccagaac atgttgtcca
     3301 caatcaggac tccacccgga cggagtttct tggcgatcac ggagagagac tgagggtagg
     3361 cctctttatc gatgtcattg aaaatcacgt cgaagggccc ctcagcacgc gagagcgtct
     3421 cgacggcctc cccaacgtgg tactgcacaa catcctcgaa ccccaaggcg cccagatgct
     3481 tgcgggcgcg gcgcgagagc tcttcgtccc agaccacgtg aaacacggtc ccgccgccgt
     3541 tctcctgcac cgcccgcgcg aaccaggccg tcgagtaacc gaaacccgat cccatttcaa
     3601 aaacccggcg agcgcgggtc agccgagcca gcagataaca caagtttcca gcggcaggtc
     3661 cgacgatcgg gaagcgtgtc tcacgcgcga aggcttccat cgcctgcatc tccgctggtc
     3721 ttgggggaac cagcgcgtcg aggtattgcc tgagttgcgg ggcaactaac tcccatacca
     3781 tctgaagcct ccacgtggga aagtgagagt gtattaaaca gaggggacgc ggttacccgc
     3841 gcccgaagtc agtgggtgcc taggatctgc gcgcccgcgt cttcctggat cgtgagtgcc
     3901 gacgtccggg cggtggcggg actttgccgc ccttacggcg cgcttccgag aggccgattg
     3961 cgatcgcctg ctcgggagac gtgaccttct tccccttccc aggaccgcgc tttccgctgc
     4021 ggagcgttcc gcgcttgcgg cgtcgcatcg ctgactccac gtgcttctgt gcggatcgtc
     4081 cgtatctggc catggatgac cccctctttc aactattcga tctggtgcaa cgtgctgcgc
     4141 cgagattgat tttgcccgct cggtagggtt caaaactcgc tatgc
//
LOCUS       DBNGSB010000094         3797 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_4204897, whole genome shotgun sequence.
ACCESSION   DBNGSB010000094 DBNGSB010000000
VERSION     DBNGSB010000094.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_4204897"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1885)
                     /locus_tag="AC159B_02625"
     CDS             complement(<1..1885)
                     /locus_tag="AC159B_02625"
                     /inference="COORDINATES: protein motif:HMM:NF042769.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HIR3376680.1"
                     /translation="MSKQTRYHLNAAFLLVLLGAATAAEAQVDQQRAQEYFKEARVLC
                     ERDGGRLWGVSICAPMVIADLRTQTIATSQPAPEGARPRGLGIVNAPVQWGGATWVAY
                     TWNDVANRTPRDRKELFLHELFHGVQKQLGLILPALATEHLDAMDGRYWLRLEWRALA
                     RALRESGEQRNLAVREALAFRQARRMLYPGSVESERCWEITEGLAAYTGTVLAAEYAA
                     DAIASAIDLLANTEAAALEASFVRTFAYMSGPAYGLLLDASSPGWTRRVRGTDDLATL
                     VMRALSVQPAADATASAARYGGAEIRASEQQREQERQERLAELRRRFVEGPVLLIPGG
                     GGGMSDSRGAVVIPGVGTVYFGPFRASGDWGTLEAEKGVLVASDGRSRRVSAPVRRDD
                     VTFTGDGWTFKAAPGWVIREGARRGDYEVVRQTTRGISAAPAPARQPSVTTDVVYGHK
                     DGLALTFDVHRPAQPNGAGVIAIVSGGWQSSVEMSRLIVDGYLAPWLNEKGFTVFAVR
                     HGSWPRYPMSSIVADVRRSVRFIRQHAKEYGVDPNRLGVFGSSAGGQLALLLGTTGDS
                     GDPSAADAVLRESSRVAAVVANFPPTDLARWATQQPVFKFSETEAAQFSPIRFVSPRS
                     APSL"
     gene            complement(2126..3379)
                     /locus_tag="AC159B_02630"
     CDS             complement(2126..3379)
                     /locus_tag="AC159B_02630"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase domain-containing protein"
                     /protein_id="HIR3376681.1"
                     /translation="MNIMQAPPTASACPPADEAIIQPQALAPSGETNLLGESLTGRAR
                     LSSVDALRGLVMVIMALDHTRDYFHIDAILFDPTDLTKTNPALFITRWVTHFCAPVFV
                     FLAGTGAFLSTSRGKTKNELARFLLTRGLWLVVLELTVIHFAWWFDFNYLSAGVIWTL
                     GWSMVVLAGLIFLPTWAVALFGVAMIASHNLSDGVRAGDFGSFHWLWTILHIPGEFKV
                     LGDIQLKVSYPLVPWVGVMAAGFGFGKLLLLERGKRRKWLLGVGGALTLLFVVIRALN
                     FYGDPSPWSGQKNGLFTLFSFINLEKYPPSLLFLLVTLGPAIAALALFDRTQEPRAFV
                     RALIVFGRVPLFYYILHLFLIHVLRVVFLYIQYNHVELLPGDTGYGLPIVYLVWFAVV
                     LLLYPACGWFAGIKKHRRDAWLSYF"
BASE COUNT          857 a         1159 c         1100 g          681 t
ORIGIN      
        1 tgagcgaggg agccgagcga ggtgagacga agcgaatggg tgagaactgc gccgcttcgg
       61 tctccgaaaa cttgaagacc ggctgctgcg tcgcccatcg agcgagatcc gtgggcggaa
      121 agttcgcaac gaccgccgcg acgcgactgg attcccttag cacggcgtcg gcggccgacg
      181 gatccccgga gtcgcctgtc gtaccgagca gcagcgcgag ctgtccgccg gcgctgctgc
      241 cgaacacgcc aaggcggttg ggatcgacac cgtactcctt cgcgtgctga cggatgaagc
      301 gaaccgaccg ccgcacgtcc gccacgatcg acgacatggg atatctcggc cagctgccgt
      361 gtctcacggc gaaaacggtg aatcccttct cgttcaacca cggggctaga tagccgtcga
      421 cgataagacg gctcatctcc acgctcgatt gccatccgcc gctcacgatc gcgatgacgc
      481 cggcgccgtt gggctgtgcc ggacggtgca cgtcgaacgt gagcgccaga ccatccttgt
      541 gcccgtacac cacatccgtc gtcaccgagg gctgacgcgc gggtgccggg gcagcgctga
      601 ttccgcgtgt cgtctgccgg acgacttcgt agtcgccccg ccgtgctcct tcgcgaataa
      661 cccatcccgg cgccgccttg aacgtccatc catctccggt aaaggtcacg tcgtcgcgcc
      721 ggacgggcgc cgacacacgg cgcgagcggc catcactcgc gacgagcacg cccttctcgg
      781 cttcgagcgt gccccagtct cccgatgcac ggaaagggcc gaagtacacc gtcccgacac
      841 caggaatcac aaccgcgccc cgggaatcgg acatgccgcc gccgcctccc ggaatcagga
      901 gcaccggacc ctctacaaag cgccgccgca attcggcgag acgttcctgc cgctcttgct
      961 cgcgctgctg ttcggacgcg cggatctcgg cgccgccgta tcgggcggca gacgctgtgg
     1021 catcagcggc gggttgaact gacagcgcac gcatcacgag cgtggcgaga tcgtcggtgc
     1081 cgcgcacccg tcgcgtccag ccgggagacg atgcatcgag caggaggcca tacgcaggac
     1141 cggacatgta cgcaaacgtg cgaacgaagc tcgcctccag agccgctgcc tccgtattgg
     1201 ccagcagatc gatggcgctg gcgatcgcgt cggccgcata ctctgctgcg agcacggtgc
     1261 ccgtgtacgc ggcgaggcct tccgtgatct cccagcagcg ctcgctttcg acactgcccg
     1321 ggtacagcat gcgtcgagcc tgccggaacg cgagcgcttc gcggacagcg agattgcgct
     1381 gctctcctga ttcccgcagt gcccgcgcga gcgcgcgcca ttcgagccgc aaccaatacc
     1441 gtccgtccat agcgtcgaga tgttcagtcg ccagtgccgg taatatcagc ccgagctgct
     1501 tctgcacgcc gtggaacaat tcgtggagga agagctcctt gcgatcacgc ggcgtcctgt
     1561 tggccacgtc gttccaggtg taggctaccc acgtggcgcc gccccactgg accggggcgt
     1621 ttacgatacc gagccctcgc ggccgagccc cttccggtgc gggctggctg gtggcgattg
     1681 tctgcgtccg caggtcggcg atcaccatcg gcgcgcagat cgacacgccc catagacggc
     1741 cgccgtcgcg ctcgcagagc acgcgggctt ccttgaagta ctcctgtgcg cgctgttgat
     1801 cgacctgtgc ctccgcagcc gttgccgcgc caagcagcac gagcaggaat gccgcgttga
     1861 gatggtaccg agtctgcttc gacatgtttc ctcctcgaga gcctgccgcc aaaccatcgg
     1921 cgcacgagtc cgacgcgtcg tgcctcagca tgcgccggtg aggtgggcgg gcgagagagt
     1981 ggtcatgatc tctatgccgc ataacgcctg gcatcacccg ccgcgcatcc aacgcatcaa
     2041 gcatacgagc tgacgaatga aagccgcgtt gttcgcggtc gggtgcatgc cgttatgcca
     2101 cccctgaaaa tcagccgcgc agttcttaga aataactcag ccaagcatca cggcgatgtt
     2161 ttttgatacc tgcgaaccac ccacaggcag ggtatagcaa tagcactact gcgaaccaaa
     2221 ccagatacac tattggtaaa ccatagccgg tatcgcccgg caaaagttcc acatggttat
     2281 actgtatata taagaaaaca accctcaaaa cgtggatgag aaaaagatgt agtatgtaat
     2341 aaaacaatgg cactcgaccg aagacgataa gcgcacggac aaacgccctc ggttcttgtg
     2401 ttctatcaaa tagggcgagc gcggcgatag ctggaccaag cgtgaccaac aggaaaagaa
     2461 gagatggggg gtatttctca aggttgatga aagaaaagag tgtaaacaag ccgttcttct
     2521 ggcccgacca aggacttgga tcgccataga aattcagcgc acggatgacg acgaaaagca
     2581 aggtaagagc gccgccgacg cctaacaacc acttccgacg cttgccgcgt tcgagaagca
     2641 acaacttacc gaatccgaag cccgccgcca ttacgccgac ccacggcacg agcggatagc
     2701 tcactttgag ttggatgtcg cccagtactt tgaattcgcc aggaatgtga aggatggtcc
     2761 aaagccaatg aaatgatccg aagtcgccag cgcgaacacc atcagataaa ttatgagagg
     2821 cgatcatcgc aacgccgaat agggcgacag cccaagtggg aagaaagatt aatcccgcga
     2881 ggacaaccat tgaccagccg agtgtccaga taacgccggc gctcaggtag ttgaaatcaa
     2941 accaccatgc gaagtggata actgttagct ccagaaccac cagccacagc ccccgagtca
     3001 ggaggaatcg cgcaagttcg ttcttagtct ttccgcgaga ggtggacaga aacgcacccg
     3061 tcccagcaag gaaaacaaaa acaggggcgc aaaagtgtgt cacccatcgg gtgatgaaca
     3121 gggccgggtt cgtcttcgtc aaatctgtgg gatcgaacaa tatggcatca atgtggaagt
     3181 aatcgcgggt atggtcaaga gccatgatga ccatgactaa accgcgaagc gcgtcaaccg
     3241 aggagagccg agcacgaccg gttaaagact cgcccagcaa gtttgtctcg ccggagggcg
     3301 ctaacgcttg aggctggatg atcgcctcat ctgctggggg acatgctgat gcagttggtg
     3361 gagcttgcat gatattcatt gctccttttc ggggaagtat aaggagcggc ataacttctg
     3421 attagatagc caaaactcgt gttgcgacac gaaacgttct catataatac gcgccgcctc
     3481 gcaagttttc tgaagattct tcactcttcc tgttgcgaca cgattccgtc gctccgagaa
     3541 gcgaagcgga cgcacagcgc aacgtgtcga agtttcttga tcaggtcagc accatcgtcg
     3601 actaccgcag tacaacagtc ctcatgaata acggttaggt ccgatctcgc ttagtctgat
     3661 ttggcccact cctagttgac aaagccaaga cctatttcgc atccacttct aaaactcaca
     3721 ccgagtccga gccgccggca gagtgtgatt ttcagaagtg aatgcgactg gaagggtcaa
     3781 aaggggcatt ttcacag
//
LOCUS       DBNGSB010000095         4550 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_1291336, whole genome shotgun sequence.
ACCESSION   DBNGSB010000095 DBNGSB010000000
VERSION     DBNGSB010000095.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4550)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_1291336"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="AC159B_02635"
     CDS             <1..379
                     /locus_tag="AC159B_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376682.1"
                     /translation="VGVLVLSHWVLDVIVHRPDLPLSLSGRARVGLGLWNSVAGTLLV
                     ELALYAVGLYLYLRATKAKDGLGRYGFWALILVLPAVFLASAFGPPPDSERALATFAL
                     AGWLFVPWGYWIDAHRQARSTYN"
     gene            410..649
                     /locus_tag="AC159B_02640"
     CDS             410..649
                     /locus_tag="AC159B_02640"
                     /inference="COORDINATES: protein motif:HMM:NF025601.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HIR3376683.1"
                     /translation="MAAIRERMKVTADKLFDTVRGLIHEGNVRKLIVRKDERTIMEIP
                     LTIAAVGVVVAPLLAAIGALAAIAGEYELEVEREQ"
     gene            765..2513
                     /locus_tag="AC159B_02645"
     CDS             765..2513
                     /locus_tag="AC159B_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR3376684.1"
                     /translation="MPSAADVLIETIQDWGVEVIFGLPGDGINGIMEALRKREREVRF
                     IQVRHEESAALAACGYAKFTGKLGVCLATSGPGAIHLLNGLYDAKLDGQPVLAITGMP
                     FHDLIGTHGQQDVEVDKVFADVAAYNARIMSSTHVENVADLACRTALSYRKVAHITFP
                     VDLQDKEAEKKGSKRNVPHHTSDVFARGARLPAEDDLRRAAEILNEGKRVAILAGQGA
                     LKATDELEQVAERLAAPVVKALLGKAAVPDDSPYTTGQIGLLGTRPSQEALEECDTLL
                     MVGTSFPYIEFLPNPGQARAVQIDIDAARIGLRYPVEVGLVGDARRALEALLPLLKYH
                     EDRRFLEKAQHGMTEWRKLMEERGSVQDTPMKPQVVAWELGKRLRDDAIVIADSGTIA
                     TWWARQIPAKRGQMHTLSGTLATMANGLPYAIAAQLAYPDRQVVAFMGDGGFSMLMAE
                     LATCVKYQLPIKIVIAKNNTLGMIKWEQMVFLGNPEYGCELQPIDFAAVARAFGATGL
                     TITDPAECGPALDEALNHPGPVVVEAVVDPYEPPMPPKITRDQALKFAKSLIRGEPNR
                     EKIALTVLSDKIRELI"
     gene            complement(2522..3085)
                     /locus_tag="AC159B_02650"
     CDS             complement(2522..3085)
                     /locus_tag="AC159B_02650"
                     /inference="COORDINATES: protein motif:HMM:NF024231.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032217 - riboflavin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0032218 - riboflavin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR3376685.1"
                     /translation="MNVRLLVLVGMLGAAAFILMATIQIPILPSAPYLRYDPSDMIGL
                     IAAFLAGPAAGVAVIALKDALYLLFRARSIFGPLANFLAVATFVGVAGWVLRGRPLTL
                     YSLLGACAAGGLARVLLMIPANFIILNLQLGMPASRVAELVWPVIIPFNTLASVINTA
                     LTAIVLLAVRKRGVTSLTTYLRDVEGR"
     gene            3159..4139
                     /locus_tag="AC159B_02655"
                     /pseudo
     CDS             3159..4139
                     /locus_tag="AC159B_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306234.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
     gene            4195..>4550
                     /locus_tag="AC159B_02660"
     CDS             4195..>4550
                     /locus_tag="AC159B_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3376686.1"
                     /translation="MKGGGIVRRWRLIVSALVAVLLLIPAAGIGQTPGSLRLAITRDE
                     GHLNPYTYQTGYPGWNLMTLVYDTLFYPDANNEPIPWLVRNAQVSRDGLTWTLTLHPN
                     IRWHDGRALTSEDVKFT"
BASE COUNT          925 a         1401 c         1388 g          836 t
ORIGIN      
        1 agtgggcgtg ctggtgctga gtcactgggt ccttgacgtg atcgtgcacc ggccggatct
       61 gccgctcagt ctcagcggac gagctcgagt cggtttaggg ctgtggaatt ctgttgcggg
      121 cacgctgctt gtggaactcg cgctctatgc agtgggcctc tacctctatt tacgagccac
      181 aaaggcaaag gacggcctgg gccgttacgg cttttgggcg ctgatcctgg ttttgccggc
      241 ggtgttcctg gcgtcagcgt tcggaccgcc gccggacagc gaacgcgccc ttgcaacctt
      301 tgcgcttgca ggatggttgt tcgtgccgtg gggctactgg atcgatgctc acaggcaggc
      361 gcgctccaca tataattgaa gagaaccgac aagggaggag aatctgacca tggctgccat
      421 tcgagaacga atgaaggtga ccgcggacaa gctgtttgac accgtacgag gattgatcca
      481 cgaaggcaac gtccgcaaac taatcgtccg caaggacgag cgcaccatca tggagatccc
      541 gttgaccatc gccgcggttg gcgtggtggt cgcgccgctg ctcgcggcta tcggtgcgct
      601 ggcggccatc gcgggcgagt acgagctgga agtagagcga gaacagtaac agtcgcacga
      661 agaaacccgg tatttccgta cgacctgacc cactaagtgg attagactgc ccgcgcgcag
      721 gcaaaacctt gtagtagttc tcctacagga agggtgatcc aactatgccg tctgcagcgg
      781 acgtccttat tgaaaccatc caggattggg gagttgaagt catcttcggt cttcccgggg
      841 atgggatcaa cgggatcatg gaagccctgc gaaagcgcga gcgagaggtc cgattcattc
      901 aagtgcgcca cgaagagtcg gcggcgctgg ccgcgtgtgg atacgccaag ttcacgggca
      961 aactgggagt ctgcctcgcc acgtccggcc ccggcgcgat ccacctgctg aacggcctct
     1021 acgacgcgaa actggacggg cagcctgtgc tggcgatcac cggcatgccc tttcatgatc
     1081 tcatcggcac acacggccaa caggacgtcg aggtggacaa agtctttgcc gatgtcgcag
     1141 catacaacgc gcgcatcatg agctccaccc acgtcgagaa cgtcgccgac ctcgcctgcc
     1201 ggaccgcgct ctcgtaccgc aaggttgcgc acatcacctt tccagtagat cttcaagaca
     1261 aggaagctga gaaaaaaggc tccaagcgga acgttccgca tcatacgtcg gacgtgttcg
     1321 cccgcggcgc caggctgccg gctgaagatg acctccggcg cgccgctgag atcctcaacg
     1381 aagggaagcg tgttgcgatc ctggccgggc aaggcgcact caaggccaca gacgagctgg
     1441 agcaggttgc cgaaaggttg gccgcgccgg tcgtaaaagc cctgctcggc aaggcggcgg
     1501 ttcccgatga tagtccctat acaacaggcc agatcggcct gctggggacc aggccatcgc
     1561 aggaagcgct cgaggaatgc gacacgctcc tcatggtcgg cacatcattc ccgtacatcg
     1621 aattcttacc gaaccctggg caggcgcgcg ccgtgcagat cgacatcgat gccgcgcgca
     1681 tcggcctgcg ctatcccgtc gaggtcggcc ttgtgggcga tgcccgccgt gctctcgagg
     1741 cgctgctgcc gttgctgaag taccacgaag atcgccgatt cctggagaaa gcccagcacg
     1801 gcatgacgga atggcggaag ctgatggaag agcgcggatc ggtgcaagac actccaatga
     1861 aaccgcaggt tgtggcctgg gagctcggca agcgtctgcg cgacgatgcg atcgtcattg
     1921 cagacagcgg caccatcgcg acctggtggg cgcggcagat cccggcgaaa cgcgggcaaa
     1981 tgcacacgct ttcgggcacg ctggcgacga tggccaatgg cctcccctat gcgatcgctg
     2041 cgcagctggc ctatccggac cggcaggtcg tcgcgttcat gggcgatggt ggattctcga
     2101 tgctcatggc tgagctggca acctgcgtga agtatcagtt gcccatcaag atcgtgatcg
     2161 ccaagaacaa cacgctggga atgatcaagt gggaacagat ggtgttcctc ggaaatcccg
     2221 agtacggctg cgagctccag ccgattgatt ttgcggcagt cgcgcgggcc ttcggggcca
     2281 caggactgac gatcactgat cctgccgaat gcggaccagc gctcgacgag gcgctcaacc
     2341 atcccggtcc ggtcgtggtg gaggccgtgg tcgatccgta cgagcctccg atgccaccca
     2401 agatcacgcg cgatcaggca ttgaagttcg cgaagtcgct tatcagggga gaacccaacc
     2461 gtgaaaagat cgccctcacc gtactgtcag acaagattcg cgagctgatc tgacgctgac
     2521 ttcagcgtcc ctcaacgtcc cgtaaatacg tcgtcagcga agtcactccg cgctttcgga
     2581 ccgccagcag cacgatggcg gtcaaagcag tgttgatgac gctggccagg gtattgaagg
     2641 gaatgatgac aggccacacc agctctgcga cccgtgatgc cggcatcccg agctgcaggt
     2701 tcagaatgat gaaatttgct ggaatcatca ggagcactcg cgccagacct cccgccgcac
     2761 aagcaccgag caacgagtat agggtgagcg gcctaccgcg taggacccaa ccggcaacgc
     2821 cgacaaaggt tgcgacagca agaaaattgg ccagtgggcc aaagattgac cgcgcacgga
     2881 agagtagata gagtgcgtcc ttcagcgcaa tgaccgcgac accagccgcc ggtcccgcca
     2941 agaatgccgc aatgagaccg atcatgtcgc tgggatcgta gcgcaggtac ggagccgacg
     3001 gcaggattgg gatctggatc gtcgccatca ggatgaaggc ggctgcgccg agcatgccga
     3061 cgagtacgag caatcgaacg ttcactccac gatctccatc ccgaacgtcc caatcggcag
     3121 gattcttgac gtcctcacgg gcaaatcccc cttgtacaat ggcgcattca cacgacagcc
     3181 acgctcacac gcacggtatt gtcgatccct ccattgtgac gtccgaacgg gggatctggg
     3241 cgatcaagtg gtctttcgtt ggcctcatga tcaccgcgct cctgcaggtc gcagtcgtga
     3301 tcgtctcaaa cagcgtcgca ctcctggcgg atacgattca caacttcggc gacgcgtcaa
     3361 cggcaatccc gctctggatc gcgttccggt tcgcacggct gaagccgaac cgcagatttc
     3421 cttacggcta cgcgcgcgtc gaggatctcg ccggcgcggt cattgtcctc acgatcctgt
     3481 tcagcgcaat cgtcgccggc tacgaatcaa tcgatcggct catccgtccc cgaccagtag
     3541 aatacctctg ggcggtggcg gccgcgtcgg ttgtgggctt tctcggcaac gaggcggtcg
     3601 ctctgtttcg gatcagggtc ggtaaagaga tcggcagcgc ggcgctcgtg gccgacggct
     3661 aacacgcccg cgttgacgga ctgacgagcc tggccgtcct cgctggagca ctcggcgtct
     3721 ggctgggttt tcccttggcc gacccactcg tcggtctcgg cattaccgcc gccatcctcg
     3781 ggatcgtctg gcaatcggcg cgcgccgtct tcacccgaat gctcgacggc gttgacccgg
     3841 atgtgatcga cgaactctcg catgcggtgc accacgtgcc gcaggtggac gacgtgacgg
     3901 acgtgcgggc ccgctggctc ggacaccggc tgcatgccga ggtgagcatt gcggtcaagc
     3961 ccaacctgac ggtggctgaa gcccatgccg ttgcgctcga agtccaccac cagctcctgc
     4021 atcacgtccc acatctcgcc gctgcgacga ttcacgtgga tcccgcgaac gcagccggtg
     4081 atcggcacca ccgggtcatt gcgcacgcgc acgacaacct gcctgtgcac tcacactaga
     4141 gaggaagccc agcaactccc cccgaaaccc gtcagacatc cgttgggcgt gaacatgaaa
     4201 ggtggtggca tcgtgcgccg ttggcggctt attgtgtccg cgctggtggc ggttctcctc
     4261 ttgatccccg cggcgggaat tggtcagacg cccggctctc tgcggctggc aatcacgagg
     4321 gatgagggac acctcaatcc gtacacctat cagaccggct acccgggatg gaacctcatg
     4381 accctcgtct atgacacatt attctatccc gatgccaaca acgaacccat cccatggctg
     4441 gtcaggaatg cccaagtctc gcgtgatggc ctcacctgga cgctcactct ccatccgaat
     4501 attcgatggc acgacggtcg ggcgctcacc tctgaggatg tgaagttcac
//
LOCUS       DBNGSB010000096         3427 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3899268, whole genome shotgun sequence.
ACCESSION   DBNGSB010000096 DBNGSB010000000
VERSION     DBNGSB010000096.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3899268"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /gene="tdh"
                     /locus_tag="AC159B_02665"
     CDS             complement(<1..986)
                     /gene="tdh"
                     /locus_tag="AC159B_02665"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - L-threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HIR3376687.1"
                     /translation="MKALTKADAGPGLELVDVPKPLLRSGEVLIQIFAGGICGTDLHI
                     FGWNEWARSRIKPPRVIGHEFCGTVVEAAPDVTALKVGDFISGEGHVACGVCRSCRSG
                     SPHICERLEVIGVDRDGAFAEFLAMPAQNAWRIEPSIPRDVAAIMDPLGNAVHTALET
                     PLVGRSVAVIGCGPIGLMAIPVCHMAGAAFVAASDVAEPRLALARRMGADLVVNAKTE
                     DVPTRIRDGNGGQGVDVVLEMSGDPHGLRDGLKVLRNGGWVSLLGLPSGPVAVDFGRD
                     VIFKEAHLVGIFGRKMWQTWEQATALLKNGLDVTPVITHRLPLARFEEAFELL"
     gene            complement(983..2617)
                     /locus_tag="AC159B_02670"
     CDS             complement(983..2617)
                     /locus_tag="AC159B_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376688.1"
                     /translation="MGTPIGAANSITRTKTRTAINDKAIDRQEGKRDALVNAAVGFIT
                     REAGKPVYRHDVIIHGVRVRATTNSDHLYDYWVDNWYSPEEWKAITGLVPPRDPQVTV
                     FALGRVSDQPEAAYYSRKTNTIVFFNTAYYGQLKSWVLGAVGRVLAEEYGIHSIHGAC
                     VEKDRRGILYIAPTGTGKSTSSYGLMHFPHTRFHSDDWVYVRYTYATEDGRRIHPMQV
                     TLPGGRELRGYPVFRWLETDAADHADATLTGLDLESREVTVPLSALDFSAPLEAHAFT
                     SEKIFYLRTNLVENFPLSAMQMLRSKMENVPEIAPAFLNQHHEMLDDLVEAIRAEGGE
                     VTQYFAEHSRDEVKHLLARMIAFDNARAMLDIGKVLPADRVFVNPMEPAKLSTVILLR
                     RANEDRAVIESLSLPSFMARLLIGETPDKKREIAYNAYRAVDDAEEQGYVKSLEEDAA
                     RRGGRVLDRLAESFVRRSDTPETLREEFELFRVMHRACRCYSLNTVLTADPQVKDKKE
                     AVALTLQLIARLVDDHPTDIQKTLDDYRSLISAPAR"
     gene            2807..3211
                     /locus_tag="AC159B_02675"
     CDS             2807..3211
                     /locus_tag="AC159B_02675"
                     /inference="COORDINATES: protein motif:HMM:NF021505.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HIR3376689.1"
                     /translation="MRLLYHPLIVHFPVALWLTSFLFDLMYVRTRQTFFTTASNYLIG
                     LGLLSAAASVISGFVDFIPLVREGVGQAFVDRHRVHSLTAYAASIVYLGILITRWRWR
                     PRSTVAYLWPALLGAVLIAIAGYLGGEIRRVM"
     gene            3311..>3427
                     /locus_tag="AC159B_02680"
     CDS             3311..>3427
                     /locus_tag="AC159B_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HIR3376690.1"
                     /translation="MSQKILVVDDEPHIVELVKYNLLQEGFDVLSAYDGSEGL"
BASE COUNT          638 a         1062 c         1064 g          663 t
ORIGIN      
        1 agcagctcga aggcctcttc gaatcgagca agcggcaggc ggtgcgtaat caccggggtg
       61 acatccagcc cgttcttcaa caatgcggtg gcctgctccc acgtctgcca catcttgcgg
      121 ccaaaaatgc cgacgaggtg ggcctccttg aagatcacgt ccctgccgaa atcgaccgcc
      181 acgggcccgc tcggcagacc gagcagcgac acccagccgc cgtttcgaag gaccttcagg
      241 ccgtcgcgga ggccgtgagg atcaccggac atctccagga cgacatccac tccttgtccg
      301 ccgttgccat cacggatgcg cgtcgggacg tcttctgtct tcgcattcac gacgagatcg
      361 gcgcccatcc gccgtgccag cgccaggcga ggttcagcca cgtccgaggc tgcgacgaag
      421 gccgcgcccg ccatatggca cacaggaatg gccatgagtc cgatcggtcc gcagccgatc
      481 acggctacgc tgcgtccgac cagaggggtc tcgagcgcgg tgtgcacggc attacccagc
      541 ggatccatga tggccgcaac gtcacggggg atcgaaggtt cgatccgcca cgcattttgc
      601 gccggcatgg cgaggaattc ggcgaacgcg ccgtcacggt cgacgccgat gacctccagc
      661 cgttcgcaga tgtgtgggga cccgctccgg cagctgcggc acactccgca cgccacgtgc
      721 ccttcaccgg aaatgaagtc cccgaccttc agcgccgtga cgtcgggagc cgcctcgaca
      781 acggtgccgc aaaactcatg gccgatgaca cgcgggggtt tgatgcgcga acgcgcccac
      841 tcattccagc cgaaaatatg gagatcggtc ccgcagatgc cgccggcgaa aatctggatc
      901 agcacttcgc ccgaccggag tagtggctta ggaacatcaa cgagctcgag gccgggaccg
      961 gcgtccgctt tggtgagcgc cttcatcgcg ccggagcgct gatgagggac cggtaatcgt
     1021 cgagcgtttt ctgaatgtcg gttgggtgat cgtcgaccag ccgagcgatg agctgcagag
     1081 tcaaggcgac ggcttccttc ttgtccttga cctgcggatc cgccgtcaat actgtgttga
     1141 gcgaatagca ccggcaggcc cggtgcatca cgcggaagag ctcgaactct tcgcggagcg
     1201 tctccggcgt gtcgctccgg cgcacgaacg actccgcaag ccggtcgagc acacgtcctc
     1261 cgcgccgggc cgcatcctcc tcgagcgact ttacgtaccc ctgctcctcg gcgtcgtcca
     1321 cggcgcgata tgcgttgtac gcgatctcgc gtttcttgtc cggtgtctcc ccgatcagca
     1381 accgggccat gaatgatggc agcgaaagcg attcgatgac cgcgcggtcc tcgttggccc
     1441 gccgcaacag gatgacggtc gacagcttcg ccggctccat gggattgaca aacacgcggt
     1501 ccgccggcaa cactttgccg atgtccagca ttgcgcgcgc gttatcgaac gcgatcatgc
     1561 gggcgagcaa gtgcttcacc tcgtcgcgcg agtgctcggc aaagtactgc gtcacctcgc
     1621 cgccctcggc ccggatcgct tcgacgaggt catcgagcat ctcgtggtgc tggttcaaaa
     1681 acgccggggc gatctcgggc acgttctcca tttttgaccg cagcatttgc atcgccgaca
     1741 acgggaagtt ctcgaccagg ttggtgcgca ggtagaagat cttttcggag gtgaaggcat
     1801 gcgcctcgag cggagccgaa aaatcaaggg ccgagagcgg cacagtcact tcacggcttt
     1861 ccaggtccag gcccgtcaga gtcgcgtccg cgtggtcggc ggcgtcagtt tcgagccagc
     1921 ggaacacggg gtagccgcgc aactcacgtc cgcccggcag cgtcacctgc atcggatgga
     1981 tgcggcggcc gtcttccgtg gcataggtgt agcgcacgta cacccagtcg tcggagtgaa
     2041 agcgcgtgtg ggggaaatgc atcagaccgt acgacgacgt gctcttcccg gtcccggttg
     2101 gggcgatata gaggatcccg cgacgatcct tctcgacgca ggcgccgtgg atcgaatgaa
     2161 tgccatactc ttccgcgagg acgcggccga cggctcccag cacccagctt ttcaactggc
     2221 cgtaataggc ggtattgaag aacacaatcg tattggtctt gcggctgtag tacgcggcct
     2281 cgggctggtc actcacgcga cccagcgcga atacggtgac ctgcggatcg cgcggtggaa
     2341 cgagtcccgt gatcgccttc cactcttccg ggctgtacca gttgtcaacc cagtagtcgt
     2401 acagatggtc cgagttggtc gtcgcgcgca cacgcacgcc gtgaatgatg acgtcgtgac
     2461 ggtagacggg ctttccagct tcccgagtga tgaagccgac ggccgcgttg acgagggcat
     2521 ctcgtttccc ttcctggcga tcgatagctt tatcgttgat cgctgtgcgg gtcttcgtgc
     2581 gggtaatgct gttggccgcc ccgatgggcg ttcccatcca ctcaatagcg tcagaagacg
     2641 gcgacgggga aaatgtgaag gactccgaca caaatgaacc ccttcgacgc ctggaatgcc
     2701 ctcccttgca agcgaaactc cacgtcaggc gaggggtttt cctgtgttac actgccgtta
     2761 cgcggaggtg acagcgccag aatgttcggg cctagactga gataagatga ggctgctcta
     2821 tcaccctttg atcgtccact ttcccgtggc gctgtggctc acaagttttc tgttcgacct
     2881 tatgtatgtc agaacgaggc aaacgttctt tacgacagcc tccaactatc tgatcggtct
     2941 tgggcttctg agcgcagctg cgtcggtgat cagcggcttt gtcgacttca ttccgttggt
     3001 tcgcgaaggc gtcggtcagg cgttcgtgga ccggcacagg gtgcacagcc tcaccgccta
     3061 tgcggcctcg atcgtgtacc tggggatcct gattaccagg tggcggtggc gcccgcggtc
     3121 gaccgtggcg tatctgtggc ctgccctgct gggggcggta ttgattgcga tcgcaggata
     3181 cctcggcggc gagatccgcc gggtcatgta aagcaaaaat gcggtcgtag gtcgtaggca
     3241 gtaggtcgtg ggtggcgttc cctacgacct acgacccacg acctatgacc aaagtcaaag
     3301 ggagggtgcg atgtcccaga agattctcgt tgtcgacgac gagcctcaca tcgtcgagct
     3361 cgtaaagtac aatctcctcc aggaaggctt cgacgtgctg agcgcgtacg acgggagcga
     3421 gggactc
//
LOCUS       DBNGSB010000097         5133 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2212320, whole genome shotgun sequence.
ACCESSION   DBNGSB010000097 DBNGSB010000000
VERSION     DBNGSB010000097.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 5133)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5133)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5133
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2212320"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="AC159B_02685"
     CDS             <1..498
                     /locus_tag="AC159B_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013921766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-disaccharide hydrolase"
                     /protein_id="HIR3376691.1"
                     /translation="RDQDFPISIEAQFLGGLGDGKTRSTMNLCTPGTEVVYRGSIHPE
                     HCLNSASKTFDGDQWVRAEMLVLGSGQITHFVNGEEVLEYALPQFGGGGVSDFDPPAK
                     PDGKLIEGGYISLQSESHPIDIRKVELLDLAGCMDRSASNYKRYHVKSVPESCVYAKG
                     SKPGE"
     gene            complement(553..1158)
                     /locus_tag="AC159B_02690"
     CDS             complement(553..1158)
                     /locus_tag="AC159B_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3376692.1"
                     /translation="MTPSSSDSQLQYSPKALEIVRRTNELLASGGYNGFSYADIAELV
                     DVRKASIHHHFPTKADLVKATVALHRDAVRRGLQSLDESTADPLERLVAYSRFWAECI
                     EGSNPPICICALLAAELPAVPAEVADEVKGHFQDLHAWIASNMEEGKSKGGMQLSDTP
                     SAEASMFMASIHGAMLSARAAGDASLFWKIAKLSTDRLRVA"
     gene            complement(1158..1652)
                     /locus_tag="AC159B_02695"
     CDS             complement(1158..1652)
                     /locus_tag="AC159B_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008957863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376693.1"
                     /translation="MGISPLGWLHTLGSLPAIPLAAYMLVKHGRIAPETRAGRAYFWF
                     MLLGVLTVYPIAHQPISSIVATVALVALLLGYGISRWPMAKRFGKYIETISLSISVFL
                     LMVPTVSESLRRLPVGDPLVTDLKDPLLLGVQGALFLALIVGIPLQVRALYKRKVLQG
                     EADS"
     gene            2052..2750
                     /locus_tag="AC159B_02700"
     CDS             2052..2750
                     /locus_tag="AC159B_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017908386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HIR3376694.1"
                     /translation="MGYAKDAASPSRIRLVRRIGWIIVWFVLGMISVNFLLTVYGKYH
                     HVDPGAYAMFWSRRGWLWTHLAGGALTIVLGPLQFLTRWPRAYPRLHRWTGRIYMVGM
                     LIACAGATGLIATSPAPFEIRTAFASTALAWLTTALIALIAIHRGRVWPHRRWMTRNY
                     LVTVSPITFRILLKALIAMELAPSPAIIATLLWLSWLLPLLVYEGAYRIVGLMRATPA
                     QQAQMGALSRSAST"
     gene            complement(2934..3497)
                     /locus_tag="AC159B_02705"
     CDS             complement(2934..3497)
                     /locus_tag="AC159B_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011643085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HIR3376695.1"
                     /translation="MPATPDDAIVSMIANYEKNTGKPFEAWLALMRKEKFAKHGEIVK
                     RLKDQHGMSHGYANLVATHVLRDDTSAATGNGLLAAQYAGTKAALKPLYDKLAAAVAK
                     FGNDVELSPKKTYVSLRRSKQFGLIQPSTADRLDVGLNLKGVAAKGRLEPSGSFNAMV
                     SHRVRITRAKDIDAELMGWLKAAYDAA"
     gene            complement(3509..4036)
                     /locus_tag="AC159B_02710"
     CDS             complement(3509..4036)
                     /locus_tag="AC159B_02710"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3376696.1"
                     /translation="MKIIKWLLIVVVVLAVLIIGIGFLLPSHYRVERSATIDASPQQV
                     YGYLADPRQWAKWTAWNRRDPAMKITYSGPSSGQGAQWAWESSEGDGSMTFTRAEPNK
                     AIEYTLVFPDMGMASTGTLTLMPAGAATQVSWTAEGDVGRNPLMRWFVPFMDRMIGPD
                     FVEGLANLKTLAEQD"
     gene            complement(4054..4572)
                     /locus_tag="AC159B_02715"
     CDS             complement(4054..4572)
                     /locus_tag="AC159B_02715"
                     /inference="COORDINATES: protein motif:HMM:NF019928.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HIR3376697.1"
                     /translation="MAEPQKAVFRIVIDGNQDAIFRELTKTDSPQGAVFNAMLTLPTP
                     GLATGKPMQMRTVSGRHTMVLGEVVEYDPPRRFAHTHRFAQHGDPACKVVYELKPVPG
                     GIEVTLTVENIPVGTKTANEMQRGGMFILNNLKSIVETGRPPFATRLMYLAFDWLEFV
                     LPKKTRSELWPL"
     gene            complement(4565..4942)
                     /locus_tag="AC159B_02720"
     CDS             complement(4565..4942)
                     /locus_tag="AC159B_02720"
                     /inference="COORDINATES: protein motif:HMM:NF024804.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR3376698.1"
                     /translation="MNADEMDAVFAALASKPRRRILDILKKEPGCNVNRVAEFFDMGR
                     IAVMKHLAILEAANLVVSEKVGRERRLWFNPVPVQMIYDRWTTDFSAYWAGDLTRIKY
                     RLENAPVPIRSTPVRKKAGGVRG"
BASE COUNT          974 a         1566 c         1564 g         1029 t
ORIGIN      
        1 cgcgatcagg acttcccgat ttccatcgag gcgcagttcc tcggcggttt gggcgatggc
       61 aagacgcgct cgacgatgaa cctgtgcacg ccgggaacgg aagtcgtgta ccggggttct
      121 atccatcccg agcattgcct caactccgct tccaagacct tcgacggcga ccaatgggtg
      181 cgcgccgaga tgctggtgct gggcagcggg cagatcaccc atttcgtcaa cggtgaggaa
      241 gtgctcgagt acgcattgcc gcagttcggc ggcggcggag tgtcggattt cgacccgccc
      301 gccaagcctg acgggaaact gatcgaaggc ggctacatct cgttgcagag cgaatcgcat
      361 ccgatcgaca tccgcaaggt ggaactgctc gaccttgccg gctgcatgga ccgcagcgca
      421 agcaattaca agcgctacca cgtcaaatcc gtgcctgaaa gctgtgttta tgcgaaaggc
      481 tctaagcccg gagagtagcg ccagcattgg ccaataacga acagccggcg gattcggttc
      541 gccgatatgc cgctatgcca ctctcaggcg atcggtagac agcttcgcaa tcttccagaa
      601 cagcgacgca tcgccagcgg cccgggcgga cagcattgcg ccgtgtatcg atgccatgaa
      661 catggaagct tccgccgacg gagtgtcgga cagttgcatt ccgcccttcg acttcccctc
      721 ttccatgttg gaggcaatcc atgcgtgaag gtcctggaag tgccctttga cctcatctgc
      781 gacttcggct ggaacggccg gcagttccgc cgccagcaga gcgcaaatgc aaataggggg
      841 atttgatcct tctatgcatt ccgcccagaa acgagagtaa gcgacaaggc gctccagagg
      901 atcggcggtc gactcatcga gcgactgcaa gccacgccgc actgcatccc gatgcagagc
      961 gaccgttgcc ttcaccaaat ccgccttggt cgggaaatgg tggtgaatgc tggcttttct
     1021 tacatcgacc agctcggcga tatccgcgta gctgaagccg ttatacccgc cagatgccag
     1081 cagctcgttc gtccgccgaa cgatctccag ggctttgggc gaatactgca actgcgaatc
     1141 cgagctggat ggcgtcatca actgtctgcc tcgccttgaa gtactttccg cttgtacaac
     1201 gcacgcacct gcaaagggat gccgactatc agcgccaaaa acagcgcgcc ctggacaccg
     1261 agcaaaagcg ggtctttcag atcggtcacc agaggatcgc cgacgggcag cctccgcaaa
     1321 ctctcgctta cggtggggac catcagcagg aaaacgctga tgctaagtga aatcgtctcg
     1381 atgtacttcc cgaaccgctt cgccatgggc caccttgaga tgccatagcc cagaagaagc
     1441 gccacaagcg cgacggtcgc aacgatcgag cttatgggct gatgcgcgat cggatagacc
     1501 gtgagcacgc ccagcaacat gaaccagaag tacgcccgtc ccgcccgggt ttcgggagcg
     1561 atgcgtccat gcttaacgag catgtaggcg gcaagaggta tggcaggaag gcttccgagc
     1621 gtatgaagcc agccaagagg agagataccc atggagtcat tccaatcgaa ggagaattca
     1681 atctacctac tagtagatag attgtcaatt gctcagccgg tcgtattgca gcgccgtgtt
     1741 gtcttgcaga cggcatctcg accggcgacg ccggccaagg tggcagcagc tcaacagcca
     1801 cgacttcgcc atggcgctgt tcgacgactt ggcaccggcg agaccgcctg tgcccggggc
     1861 ccagtagttc aagaagctgc tggcgggcga tgacatgttt agcccgccct acaacacaac
     1921 gctggccgaa tggcgcgatg gccgtatcgt cgggctgccc tcgcccaagc cggcgaggta
     1981 gcagcgaggc acgcttcaat cacatggtcg cccctatgta tcctttggcc agatacgagc
     2041 ctgaggtaga tatgggttac gcgaaggatg ccgcttcacc tagccgcatt cgccttgtca
     2101 ggcgtattgg ctggatcatc gtatggtttg tactcgggat gatcagcgtc aacttcctgt
     2161 tgaccgtcta tggcaagtac caccatgtgg atcccggcgc ctacgccatg ttctggtcgc
     2221 ggcgaggctg gctatggacc caccttgccg gcggggcgct gactattgtc cttgggccgc
     2281 tgcagttcct gacccggtgg ccgcgggcct atccgcgcct gcatcgctgg acgggccgaa
     2341 tctacatggt cggcatgctc attgcctgcg ccggggccac agggctgatc gccacttctc
     2401 cggctccgtt cgaaatccgc acggcgtttg cctctaccgc gctggcgtgg ttgacgacgg
     2461 ccttgatcgc gctgatcgcg atccatcgcg gcagggtgtg gccgcaccgg cgctggatga
     2521 cccgcaatta tcttgtgacg gtctccccga tcaccttccg gatacttctt aaagccctga
     2581 tagcgatgga actggcgccg tcgccagcaa tcatcgcgac attgctctgg cttagctggc
     2641 tgcttcctct gctcgtctat gagggtgcct atcgaatcgt cggcttgatg cgcgcaacac
     2701 cggcacaaca ggcgcaaatg ggcgcactct cccgcagcgc atcgacgtag cgtccgctta
     2761 gggtcggaag gagacatcgc ctggcgaccg ctccgacacc agtagatcag acgccgatct
     2821 gctcagccat ggttagcgcg tcatctatct cgtatggcca agcaatagtt gcatcgccca
     2881 acgttcttcg ttcgctggta gatgagcgct gccttcgaca gcagtaccgc ctttcaggcc
     2941 gcgtcgtaag ccgccttcag ccagcccatg agttcggcat cgatgtcctt tgcacgcgtg
     3001 atccgcacgc gatgggagac catcgcattg aaactgcccg aaggctcgag ccggcctttc
     3061 gcggcgacgc ccttcagatt gagcccgacg tcgagacgat cggccgttga tggctggatc
     3121 aggccgaact gcttgctgcg gcgcaggctc acgtaggtct tcttcggcga gagctccacg
     3181 tcgttgccga atttggcgac ggctgcggcg agcttgtcgt acagcggctt gagcgcggcc
     3241 ttcgttccgg cgtactgcgc agccagcagg ccgttgccgg tggccgcgct ggtatcgtct
     3301 cgcagcacgt gcgtggcgac gaggttcgcg tagccatggc tcatgccgtg ctgatccttg
     3361 aggcgcttga cgatctcgcc gtgcttcgca aacttctcct tccgcatcag cgcgagccag
     3421 gcctcgaacg gcttgcccgt gttcttctcg tagttggcga tcatcgacac gatggcgtcg
     3481 tcgggcgtgg cgggcatggc gcgtctcctc aatcctgttc cgcgagtgtc ttcaggttcg
     3541 cgagcccctc gacgaagtcg gggccgatca tgcggtccat gaacggtacg aaccagcgca
     3601 ttaacgggtt tcggcccacg tcgccttcgg cggtccacga cacctgggtt gcagcgccgg
     3661 cgggcatgag cgtcagggtg ccggtggacg ccatgcccat gtcggggaag acgagcgtgt
     3721 actcgattgc cttgttcggc tcggcgcgcg tgaatgtcat gctgccgtcg ccctcgctgc
     3781 tctcccacgc ccattgcgca ccctggccgg acgatggacc ggagtaggtg atcttcatgg
     3841 caggatcgcg gcggttccag gcggtccatt tcgcccactg ccgcggatcg gcgagatagc
     3901 cgtagacctg ctgcggcgag gcgtcgatgg tcgcgctgcg ttcgacccgg taatgcgagg
     3961 gcagcaggaa gccgatgccg atgatgagga cggccaacac gacgacgacg atcagcagcc
     4021 acttgatgat cttcatgggt tctctccggg tggtcagagc ggccagagtt cggatcgggt
     4081 cttcttcggc aacacgaatt cgagccagtc gaacgccaga tacatcagac gtgtcgcgaa
     4141 gggcgggcgt ccggtctcga cgatcgactt gaggttgttg aggatgaaca tgccgccgcg
     4201 ctgcatctca tttgcggtct tggtgccgac cgggatgttc tcgacggtca gcgtcacttc
     4261 gatgccgccg gggacgggtt tcagttcgta caccaccttg caggcggggt cgccgtgttg
     4321 ggcgaaacgg tgggtatgcg cgaaacggcg tggcggatcg tattcgacga cttcgcccag
     4381 caccatggtg tgccggccgc tcaccgtacg catctgcatc ggcttgccgg tggccaggcc
     4441 cggtgtcggc agggtgagca tggcgttgaa caccgcgccc tgcggtgagt cggtcttggt
     4501 cagctcgcgg aagatcgcgt cctgattgcc gtcgatcacg atacggaaca ccgccttctg
     4561 tggttcagcc acggacgcct ccggcttttt tgcggacagg ggtcgatctg atggggacgg
     4621 gcgcattctc gagtcggtac ttgatacggg tcagatcgcc agcccagtac gcgctgaagt
     4681 cggtcgtcca gcggtcgtag atcatctgca ccggcaccgg gttgaaccac aaccggcgct
     4741 cgcggccgac cttttcagag accaccaggt tcgcggcttc caagatggcc aggtgcttca
     4801 tgacagcgat gcgccccatg tcgaagaact ccgcaacccg attgacgtta cagcccggtt
     4861 ctttcttcag gatgtccagg attcgccggc gcggcttgga agcaagcgca gcaaagaccg
     4921 catccatctc atcggcattc atgtcaccag aaggtgacat atagtattgg cgctgtcaac
     4981 acccacgaac ctatcgcggt acgtggaaga tcgagacatc ccgaatcttc gttccattcg
     5041 gggacgagaa cccgcgccga catgactgtg tggttgccca acaggcaaca gactcgcagg
     5101 agcaaagtag cttgacgcta actcagtttg agc
//
LOCUS       DBNGSB010000098         3808 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3747726, whole genome shotgun sequence.
ACCESSION   DBNGSB010000098 DBNGSB010000000
VERSION     DBNGSB010000098.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3747726"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="AC159B_02725"
     CDS             <1..797
                     /locus_tag="AC159B_02725"
                     /inference="COORDINATES: protein motif:HMM:NF015971.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HIR3376699.1"
                     /translation="RLGTEDPRVTAFNGRYYICYTAASLYPATEPVRRTRPSPFTDVG
                     VPWRVRVAIATTKDFRHYRRMGLAFKQWDNKNGALFPAKIRGKYYILHRIFPNVHLGV
                     SRDLRRWHNLGPLLEIRPDMWDNNRVGAGAPPLWTPYGWLLFYHGVDMQRTYRLGMAL
                     LDLEHPRHVLARAHNPILEPTESYEKEGLVPNVVFTCGAVELGDRYFVYYGSADSVIG
                     AASVSRDAVLRWASQVASAAPQVPEHFRRAARREVRELEVVRRASG"
     gene            complement(1880..3100)
                     /locus_tag="AC159B_02730"
     CDS             complement(1880..3100)
                     /locus_tag="AC159B_02730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3376700.1"
                     /translation="MMEQLSAVDISKAFLQRISAAYVSTFPPRECGIATFTRDLTRAI
                     QIYNPFARPQIVALNDEAEIYNYPGHVRWQIAGEDPESYREAARAINASPVDIVSIQH
                     EFGIFGGKWGAYLLVFLKDLRKPVVTTLHTVLPDPPRAAVDIIRALYARSAATVVMTP
                     AAREILHRDYGIDGEQLRIIPHGVPAVSMQDPAEAKARVGLSGRMVVSSFGLINSGKG
                     IEDVLDALPSVATRFPQLLYLVLGETHPVVRREQGEEYRMRLVRQVHRLGLEPHVKFN
                     NRYLSDDELVRYLMATDVYITPYHNPNQIVSGTLSYAMGCGRAIVSTPYLYAKDALGD
                     SRGVLIPFRDPKSIAEALILLLGNPGLRQQIQQRAFAYGKQMRWPRVAAAYLSLFREV
                     RTRAEERSTAVASY"
     gene            3382..>3808
                     /locus_tag="AC159B_02735"
     CDS             3382..>3808
                     /locus_tag="AC159B_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase associated/transthyretin-like
                     domain-containing protein"
                     /protein_id="HIR3376701.1"
                     /translation="MKLAIANGTIEGRIGLLAKDGLIPCGGARVYLLLRPLDVDSVKR
                     KVIEKHGHYIPHLAHMYLDVVISSPEIQQRVSYARTRTEGDGSFLFKNLPPDRWYYVT
                     AQALTEQVMVSWQIGVYLREAERVQVVLTNTNAALPAYLP"
BASE COUNT          754 a         1185 c         1168 g          701 t
ORIGIN      
        1 agcggctcgg caccgaagat cctcgcgtca ccgcattcaa tgggcgctac tacatctgct
       61 acactgccgc gtcgctatat ccagccaccg agcccgtccg gcgcacccgc ccttccccat
      121 tcacagatgt gggggtcccc tggcgcgtgc gtgtcgcgat cgccacaacg aaagatttca
      181 ggcactaccg ccggatggga ctggcgttca agcagtggga caataagaac ggcgcgttat
      241 ttccggccaa gattcggggt aagtactaca tcctgcaccg catcttcccc aacgtacatc
      301 tcggggtttc gcgagatctc aggcggtggc acaatctcgg accgctgctc gaaatccggc
      361 cagacatgtg ggacaacaac cgcgtcggcg ccggcgcgcc gccgttgtgg acgccatacg
      421 gctggctcct cttctatcac ggggtggata tgcagcggac ataccggctg ggtatggcgc
      481 tcctggacct ggagcatccc cgccacgtgc tggcgcgggc gcacaatccc atcctggaac
      541 ccaccgagtc ctatgaaaag gaaggattgg ttcctaacgt ggtgtttacg tgcggagccg
      601 tcgagctcgg agaccgctac tttgtgtact acggcagcgc cgacagcgtt atcggggccg
      661 catcggtgtc acgggatgct gtcctgcggt gggcttcgca ggtggcgagt gctgcgcccc
      721 aggttcccga gcactttcga cgcgccgcgc gccgcgaggt tcgagaactc gaggtcgtcc
      781 ggcgggcaag cggctgatcg gaagctctct tgctgcaacc gtcagcctcg cgatgagata
      841 acagatcgtc gactctgacc ccgcgttgag gttcacgccc tgtggtgtga gcccatcatg
      901 acaggctccg gtctcaggat cataaagcgg caatcccaac ttgtttgctc cgagaaacca
      961 cgcgaaggcg tcgcgggcaa gtggacggta gcgcgaatct ttcgtcatca ccgccgccgc
     1021 cgctgcagct tcaaccatgc tcgccgcgtc gatcggctgc tggtcgtaga ccgccctcct
     1081 gccgcctcgc cggtaccacc cgctgtttcc gacagctgag aaaacccggc ctcgtggggc
     1141 ggcgtcgaat agcgtgtcct ccagaaacgc cagcacacgc tgccccgctt ccaagtaacg
     1201 ggtatcaccg gtgagcgccg ccgcgtagaa gaagccgagg ggcagtcgtc caggctcata
     1261 ggtcagaatg tcatcgaacc aaacccactc tgggaccgaa tgcttctcga accgagacag
     1321 caggttatcg gcgcatctca cagcttcgtc ccagaccatg cccgcatcca tgtgacgggc
     1381 ccacagggcc aatccctgga tcgcgtttgc ccagccgcgc gggtgaacga tgcgcgtcaa
     1441 atgaggcacc gcccgctgga agatggacgc cgcggcgccc ctcatcgtct cgggcagatc
     1501 ggaggcgaca acttcgccgc atgcccacag acatcggccg aaaccatcat ccgacacgcc
     1561 cgactgggac ggcgaccgtt cgtaggaaac ctcgtgcgcg agggtaccgt ccgggcgctg
     1621 tgcgtaaagc agaaacttca gataagtgct ggcgagtcgc tcgcgtacgg ggccgcgcgg
     1681 cagccttgca gcgacgatca atgcccgcgt gttgtcatcg gtggtgtagc cgctgtgggg
     1741 gtcgccgacg ccgaacttcg caaactggag gactcccgta ccgtcggtca gccgctcgag
     1801 gtggtcgagc ttaacgggcg gaagtctcat cgattgtcgt tggctacccg gctaccctgg
     1861 ctacctggct gccgggtttc tagtaggacg cgaccgccgt gctacgttct tccgcacgcg
     1921 tccgcacttc gcggaaaagg ctcaagtatg ccgcggccac acgaggccag cgcatctgtt
     1981 tgccgtacgc gaaagcccgc tgctggatct gctggcgcaa accaggattc cccaacagca
     2041 agatgagcgc ttcagcgatc gacttcggat cacggaacgg aatgaggacc ccgcgagagt
     2101 cgcccagcgc atccttcgcg tacagatacg gagtagagac gatcgcgcgt ccgcagccca
     2161 tcgcataaga gagcgtgccg ctgacgatct gattgggatt gtggtacgga gtaatgtaga
     2221 catccgtcgc catcaggtac cgcacgagct catcgtcgct gaggtagcgg ttgttgaatt
     2281 ttacgtgggg ctccaggccg agccggtgga cctgccgcac gagccgcatc cggtactcct
     2341 cgccctgttc gcggcgaacc acggggtgag tctcgccaag caccaggtag agcagctgcg
     2401 ggaagcgcgt tgcgacactc ggcagggcat caagtacgtc ctcgatcccc ttgccggaat
     2461 taatgagacc aaatgaggac acgaccatcc ggcccgagag ccccacgcgt gccttcgcct
     2521 cagcagggtc ctgcatcgac accgcgggga cgccgtgggg gatgatccgc aactgttcgc
     2581 cgtcgatccc gtagtcgcga tggaggatct cccgcgcggc cggggtcatc accactgtcg
     2641 cggcgcttcg cgcgtagagg gcgcggatga tgtccaccgc agcacggggc gggtctggca
     2701 gcacggtatg caaggtcgtg accacgggct tgcgcaagtc cttgaggaat acgaggagat
     2761 aggctcccca cttgcctccg aagatcccga actcgtgctg gatactgacg atgtcgaccg
     2821 gtgacgcgtt gatggcccgg gccgcctcac ggtaactctc gggatcctcc ccggcgatct
     2881 gccatcggac gtgcccggga tagttgtaga tttcggcctc gtcgttgagc gcgacgatct
     2941 gaggacgcgc gaacgggttg tagatctgga tggcgcgtgt caaatctcgc gtaaaggtcg
     3001 cgatcccgca ctcgcggggc gggaacgtcg acacgtaggc agccgagatc cgctgcagga
     3061 aagctttgct gatgtccact gcggacaatt gttccatcat cacacgcgac ttaatagaag
     3121 cggattaaaa caattatata aggtaaatgc attcaggtca cctaacactt ttacccagtg
     3181 caaagagcgc tcactgtgac gaactcagag attgccggca ttggttgaag cggaaaacgc
     3241 agcccggggt cgagaggaga ctagccggcg ggcccccggg cataatgtaa ctgggggcat
     3301 tccataagct tcgacaccgc ccatcctgcc tagttctgcc tggagcaccg tatcttccta
     3361 acgcgaaggc agccagagta tttgaagctg gccatcgcta atgggacgat cgaaggccgg
     3421 atcggcctat tggcgaagga cggcctgatc ccgtgcggag gagcccgcgt ttacctctta
     3481 ctgcgtccgc tggatgtcga ttcagtcaag cgcaaggtca ttgagaaaca tgggcactat
     3541 atcccccacc tggcccacat gtatctggac gtggtgatta gcagcccaga aattcagcag
     3601 agagtctcgt acgctcgcac ccggactgag ggggacggaa gcttcctttt caagaacctg
     3661 ccccccgacc gctggtacta cgtcacagca caggcgttga ccgagcaggt gatggtcagc
     3721 tggcagatcg gcgtgtacct tcgcgaggct gagcgggtgc aagtcgtgtt gacgaacacc
     3781 aatgcggctc tacccgcata tctgccgg
//
LOCUS       DBNGSB010000099         4373 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3749118, whole genome shotgun sequence.
ACCESSION   DBNGSB010000099 DBNGSB010000000
VERSION     DBNGSB010000099.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4373)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3749118"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..845
                     /locus_tag="AC159B_02740"
                     /pseudo
     CDS             165..845
                     /locus_tag="AC159B_02740"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
     gene            853..1779
                     /locus_tag="AC159B_02745"
     CDS             853..1779
                     /locus_tag="AC159B_02745"
                     /inference="COORDINATES: protein motif:HMM:NF022727.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3084 domain-containing protein"
                     /protein_id="HIR3376702.1"
                     /translation="MELGVFLIPVLIVLSGAIALVGNAVGRNIGRQRLTIFGLRPRYT
                     AQLITVLTGMLITITTLTVVLLMSREARVALFRLNEVLRETTRLEQEIKRQQERLKQL
                     ALGDIAYLNNQEVVREVIDGRQPTAVVRERVDALVDRAEDLARANGIGTDGNGQTIVL
                     NPPRVTWDVIASLVDQRNVETVLRLVTNQNTLRGEPLEVYVQLFDNALVFRSGGMLAS
                     ATVDGRRSRAEVEQALLRLADQAAQRARGKVLAPPGGVVTSPPSAIVDIDEHRRATTR
                     ILQARGHVLVRVVAPRDVSTIGPLLIAYRFGR"
     regulatory      1841..2062
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2091..3908
                     /locus_tag="AC159B_02750"
     CDS             2091..3908
                     /locus_tag="AC159B_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR3376703.1"
                     /translation="MVHPRLSRRLSCAWTLLFLGVLALANSEKTSWAQEPPTFELPEV
                     TVPGRRSQPVTTTPASISVLTREDLERLGMLTIGEALAYVTDVHIRQQGGLGALSLPS
                     IRGAAPSQVLVLIDGVPVNGPMTGVFDLSTISVAQVRRVEILKGPFSALYGGEGLGGV
                     INIVTIEGGSEVAARAGSHGTTSAGGRWTSSNGAVSISADRLASSGFRPNSDVATMTI
                     SGRLLWQRTSSSQFTLLINHVRSELGVPGPTAFPSPQARQGEARTILSGRWERDDPGG
                     QWIAHGFYWTGAFHFTDPAFAVDSRIDTQVGGAVAQRVWRRSAGHLQTAGFEARSLAL
                     QHNGAVGNRQAAIGALFVQDDRQISPRTLLSTGLRYDVHSIFGSHLNPRLGVVHLARD
                     GLIIRAALGRTARGPTFSELYFAPFNNPGLRPESAWSADASVAWRIRPGLELKAGAFL
                     TEATDLIRPDAAFVPQNIGRAGISGGSIDLAGQLTGRLSGSVTVSTVSAIDRATGAQL
                     LRVPWVTAAAALHYRISEHITVTALAQLVGPRPDSDPATFTTVLMPAYAVAHFRITGT
                     SGGARWQVGIDNVFEATYEPIAGFPVPGRSFFVAFSAGF"
BASE COUNT          802 a         1359 c         1424 g          788 t
ORIGIN      
        1 ggaacgcgca tctgcgattg tacggcgcgg tcctcgaggt tgagaccatc gcggaggtcg
       61 gcgatctgct ggtcgcgcga aagaacggca acacgccctg ccggcagccg ctggtcggaa
      121 ggtgagggga gcctacggct gctagctcct cgccgcggct cacagtaatc gtccccacgt
      181 acaatgagcg agaatccctg cccgcgctca tccgccgcct cgaagagctc ggagagaacc
      241 gcatcgagat cgttgtggtg gacgatgcgt ctccggatgg aacaggaacg cttgcggaaa
      301 gccttggaca acagcacgcg ctgccgattc gagtcctcca ccgcacggac aagcgaggcc
      361 tcgcgtctgc cgtgatcgat ggggcatcgg tggcgcgtgg cgagatcctc acggtgatgg
      421 acgccgatct ttcgcatccg cccgaggcgc tgccggcgct gctgtccgcg attgacgcag
      481 gcgccggaat tgccatcgca tcgaggtacg ttcccggcgg cggcatccgt ggatggccgc
      541 ctctgcggcg gctgatcagc cgcgcggcta cggggctggc acggaggatg ctgcgactcg
      601 acgtgcgcga cccgctgtcg ggattctttg cggtacgccg tgagctgctc accgattcgc
      661 aatacctcgg ggtaggatat aaacttcttt tggaggtact cgcggcccat cgaggccggc
      721 ggatcgctga agtgccttac gaatttgcgg accgcaggct cggccagagt aaactctcat
      781 cgggagagat ctggtcatat ctgaaactgc tgctgtattt gaaacgcggc cgcgggacta
      841 actagcctca tcatggaact cggtgtattt ctcatccctg tcctcatcgt tttgagcggc
      901 gcgatcgcgc tcgtggggaa tgccgtcggc cggaatatcg gtcggcagag gctcacgatt
      961 tttggcctgc ggccgcgcta caccgcccag ctcattacgg tcctgacggg gatgctgatc
     1021 acaatcacca cgctcacggt tgtccttctc atgtcacggg aggcgagggt ggcgttgttc
     1081 agactgaacg aagtattgcg cgaaacgacc aggctcgagc aggagatcaa gcggcagcaa
     1141 gagcggctca agcaactggc actgggtgac atcgcctatc tgaacaacca ggaagtcgtc
     1201 cgcgaggtga tcgacgggcg gcaaccgacc gcggtcgtca gggaacgcgt cgatgcgctc
     1261 gtcgaccgcg ccgaggacct ggcccgcgcg aacggtatcg gaaccgacgg aaacggccag
     1321 acgattgttc tcaaccctcc gagggtcacg tgggatgtga ttgcttctct ggttgaccag
     1381 cgcaatgtcg agactgtgct gcgcctcgtg accaaccaga acacactgcg gggcgaaccg
     1441 ctggaggtgt atgtccagct cttcgacaac gcgctcgtgt tccggtcggg gggcatgctc
     1501 gccagcgcga cggtcgatgg ccggcgatcc cgagcagagg ttgagcaggc cttgctgcgc
     1561 ctcgcggatc aggccgcgca gcgcgcgcgg gggaaagtct tggcgccacc gggcggtgtc
     1621 gtcacctcac cgcccagtgc gatcgtggat atcgacgaac accggcgcgc cacgacgcgc
     1681 atccttcagg cgcgcggcca cgtgctcgtt cgggtcgtcg ctccgcgcga cgtgtctacc
     1741 ataggtccat tgttgatcgc gtatcgattc ggaaggtaaa aggaaaaagg attaaggaaa
     1801 aaggaataag gaaagaagtc gcttgacctt ttaccattta ccatttacct ttttccttgg
     1861 ttggggaata gggaagccgg tgtgaatccg gcgcggtcgc gccgctgtaa ccggtgagcc
     1921 ctcctcatta gtcggggtcc ccgggaaatt cgcgtgagcg agtttctagg gtgaccatgc
     1981 cactgggcgt gtgtgcccgg gaaggcatga ggagcggcaa tgatccggaa gccagaagac
     2041 ctgcccagcc taatcgccac acgccttcgc ggcaagagga ggtgggcggg atggtacatc
     2101 cccgactgtc caggcgcctt tcgtgcgcgt ggacgctttt atttcttgga gtgctggcgc
     2161 tggctaattc ggaaaagacc tcctgggcgc aggaaccgcc gacgttcgag ctccctgaag
     2221 ttaccgttcc cggccgtcgt tcgcagccgg ttacgacaac gcctgcgtcc atcagcgtgc
     2281 tgacgcgcga ggatctcgag cgcctgggca tgctgacgat tggggaagcg ctggcctatg
     2341 tgaccgacgt gcacatccgc cagcagggtg ggctcggcgc tctcagcctg cccagcatac
     2401 gcggcgccgc gccaagccag gttctcgtgc tcatcgacgg cgtgcccgtg aacggaccga
     2461 tgacaggtgt attcgacctc agcaccatca gcgtcgccca ggttcgcagg gtggaaatcc
     2521 tcaaaggccc tttctcggca ctgtacggag gggaagggct cggcggcgtc atcaacatcg
     2581 tcacaatcga aggcggcagc gaggtcgcag cccgcgcggg atcccacggc acgacgtctg
     2641 caggggggcg ctggacttcc tcaaacggcg cggtgtcgat cagtgccgac cggctggcct
     2701 cgtctgggtt tcgaccaaac agtgacgtcg caacgatgac gatctcagga cgtttgttgt
     2761 ggcaacgcac aagcagctcg cagtttacgc tgctcataaa tcacgttcgc agcgagctgg
     2821 gtgtgccggg gccaaccgca tttccttccc cacaggcgcg acagggagaa gcgcgcacaa
     2881 ttctctccgg ccggtgggaa cgcgacgacc cgggaggcca gtggatcgcc cacggtttct
     2941 actggaccgg ggcattccat ttcaccgatc cggcgtttgc agtcgacagc aggatcgata
     3001 cgcaagtggg gggcgccgtc gcgcagcgcg tctggcgtcg cagcgccggc catctccaga
     3061 cggctgggtt cgaggcgaga agccttgcgc tccagcacaa cggcgccgta gggaaccggc
     3121 aggcggctat cggcgcgctg tttgttcaag acgatcgcca gatcagccct cgaacactcc
     3181 tgtccacagg actgcggtac gatgtgcact cgattttcgg aagtcacctg aacccgcggc
     3241 tcggggtcgt tcatctcgcg cgtgatgggc ttataattcg cgcagccctg ggtcgcaccg
     3301 cgcggggtcc gacattcagc gaactctatt tcgcaccatt caataatccg ggcctccgtc
     3361 cggaatcggc atggtcggcc gacgcttcgg tggcatggcg cattcggccg ggcctcgagc
     3421 tcaaggccgg cgcgtttctc accgaagcga ccgacctcat ccggcctgat gccgccttcg
     3481 taccgcagaa catcggccgc gccggcatca gtggcggatc catcgacctg gcggggcaat
     3541 tgacggggcg gctttcggga tcggtgacgg tcagcactgt aagcgctatc gatcgcgcca
     3601 ccggagcgca actgctgcgc gtgccgtggg tgacggcagc tgcggcgctg cactacagga
     3661 tcagcgagca catcaccgtg acggcactgg cgcagcttgt gggcccgcgg cccgactcgg
     3721 atcccgcgac attcacaacg gtgctgatgc cagcctacgc ggtcgctcat tttcgtatca
     3781 caggcacctc gggtggcgcg aggtggcagg tggggatcga taacgtgttc gaagccacct
     3841 atgaacccat cgcaggtttc cccgtcccgg ggaggagctt cttcgttgcg tttagcgcgg
     3901 gattctaata tatgatcagg cggcgcattc tgtggctatt cgtcgggacc accggcctgg
     3961 tgctggcgat agcgttgacc gtcaatgggc tgcgcgccag gccccgtgac cacattgaac
     4021 gagccgccgg gccttcagcc atcgaggaaa gcgacgaaga cggtgttcgg ggttcggggt
     4081 tgggggttcg gcaccccatt ggactcgaac cccgaacacc gaaccccaaa ccccggtcag
     4141 tgaagaaagc gcggcactcg acgccgtcac ctctaaggcg tacggcggac gcgcccagcg
     4201 gggagggagc cgcaccgaag cgggcgccaa tggtcgcacc ccctcaaagg tacccgcaac
     4261 cttcacccgt gccgcaaggc ttgcccgagg cggccgcgcc gcagccgccc tcagaaaacc
     4321 gtcccgcaga tgacgcactg cctgcgccgc cgccttcgga gaccatcgga ccc
//
LOCUS       DBNGSB010000100         5072 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3903410, whole genome shotgun sequence.
ACCESSION   DBNGSB010000100 DBNGSB010000000
VERSION     DBNGSB010000100.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 5072)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5072)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5072
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3903410"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="AC159B_02755"
     CDS             <1..877
                     /locus_tag="AC159B_02755"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HIR3376704.1"
                     /translation="NAGKSSLFNALTRAGAAVAGYPFTTIDPHHGIAGVPDPRIEAVA
                     GVTHPDRKVPATVEVVDIAGLVRGAHSGEGLGNQFLGHIREVDAIVHVVRCFTAADVP
                     HVEGVVDPMRDIEIVETELALANLSKPVLYVANISEDDLPAGPCAELVVRHAQSQEAI
                     ALPLSVKLEAEVAELGGREGDELLRAYGLTQPGLFRLIRAAYDLLSLVTFFTTASREV
                     RAWAVPSGTRAPEAAGKIHTDMQRGFIKAEVIGWRELVEAGSLQEARDRGLVRLEGKE
                     YVVGDGDVMLFKFAV"
     gene            958..1350
                     /gene="rpsF"
                     /locus_tag="AC159B_02760"
     CDS             958..1350
                     /gene="rpsF"
                     /locus_tag="AC159B_02760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIR3376705.1"
                     /translation="MKSYDLVFVIRPDLEQDASKTLVDRVTQRITDQGGTVEAVDTWG
                     KRRMSFSLKKNREGLFIHTRFSLDPSKVAEVRRGVNLIEEVLRFTLTNAVGKTPEPKS
                     AEVPAPAAPVPVTPTEPPAPTTPSAAGA"
     gene            1352..1756
                     /locus_tag="AC159B_02765"
     CDS             1352..1756
                     /locus_tag="AC159B_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014345612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR3376706.1"
                     /translation="MLNRIVLIGRLTRDPELRYVPSGHPVASFTLAVDRPFVNQQGER
                     GTDFIDVVAWRRLAEQVTQHLNKGRLVAVEGRLQIRSYETQDGQKRKVAEVVADGVRF
                     LDRKAVGAGAPTETPAQDGEPESAEGEDDVPF"
     gene            1775..2044
                     /gene="rpsR"
                     /locus_tag="AC159B_02770"
     CDS             1775..2044
                     /gene="rpsR"
                     /locus_tag="AC159B_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIR3376707.1"
                     /translation="MPEGDRDRGDRRGKKEWRGRRPKRKNCQFCSEKAQFIDFKDVMR
                     LRRFVTERGKILPRRVSGNCARHQRALAGAVKRARELALLPYTAE"
     gene            2088..3044
                     /locus_tag="AC159B_02775"
     CDS             2088..3044
                     /locus_tag="AC159B_02775"
                     /inference="COORDINATES: protein motif:HMM:NF021506.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HIR3376708.1"
                     /translation="MGIRGLTEGAILATLVALLALAARYLPVIQFAAALICPIPLTFL
                     VIRQGIRVAVLAALVAAAVGAIAGGPLTGVAIVATFAPIGIALGLGVRNRWVPGRIVL
                     VCTAVATASLLINFGLTLFIAGVNPYTVMFESLQQGQEQSLRLYERLGIDRKNLEQAS
                     GMMKQWLELMPRLIPLLIVGGAAFVALINFHVGRLVLRRFGIEVAALPPLSRWRTHAL
                     VLWTLPMGFLLSVLGQGRNPAVETAGLNLTLFAQTLLLLQGLLVAWSFFDRYGSPNWM
                     RWIMIAIAFTQPILNLVIFVLGLADAVFDLRGRWRLAREART"
     gene            3041..3487
                     /gene="rplI"
                     /locus_tag="AC159B_02780"
     CDS             3041..3487
                     /gene="rplI"
                     /locus_tag="AC159B_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HIR3376709.1"
                     /translation="MKVILLKNVPGLGERGQIKDVKDGYAFNYLLPRELAAEATPGNV
                     RSLESQKKAVEDRSKREHEDTEQLASQISQAVLEIRTRAGEGGRLFGAVTAQNVADAL
                     AGQGVRVSKRQVELDEPIKTAGFYKVPVRISPGVVVRVEVNVVGTA"
     gene            3488..>5072
                     /gene="lonC"
                     /locus_tag="AC159B_02785"
     CDS             3488..>5072
                     /gene="lonC"
                     /locus_tag="AC159B_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lon family ATP-dependent protease"
                     /protein_id="HIR3376710.1"
                     /translation="MHRVNKIVPAINPTGSLSGRETVRRKVAALFGILEGIHGAERLR
                     RRAEKLSALGLMRSRQLGRRVLALQRLVFDDPQITTIPREPEIPAILEDLQERVADLM
                     ARKSVETELDRRVADRMRERQDDYLREIRLQMLQETAGPETPATRRKLAELEQLEQVR
                     LARTALEALRPKRLGHVVGQDRAIRSLLAKLAVPIPQHVILYGPPGVGKTTAARLVLE
                     EAKQLRHTPFGEDAPFVEVDGSTLRWDHREATNPLLGSVHDPIYQGTRRDFADAGIPE
                     PKLGLVTKAHGGVLFIDEIGELDPVLQAKLLKVLEDKRVRFESSYYDRDNPHLPAYVR
                     RLFDEGAPADFVLIGATTKEPEDISPTLRSRCAEVFFDPLSPEDIQRIVRQAGRRLQV
                     EVGTDVPKTISDYTIEGRKSVSMLADAYGAGLYRRRGSGGLRITLRDLHDVIRSGRLS
                     ASAPAKASAVPEIGRVFALGVTQYVGSIIEIEATAFPVGKRQRGTIRFNETAGSMAKD
                     SVFNAASVIRRLTGIDVSHF"
BASE COUNT          984 a         1487 c         1652 g          949 t
ORIGIN      
        1 caacgccggt aaatccagcc tgttcaatgc gctcacgcgc gcaggagcgg ccgtggcggg
       61 gtacccgttt acaacgattg acccgcatca tgggatcgcc ggcgtccctg atccgcggat
      121 cgaagcggtc gccggcgtca cgcatcccga tcgaaaggtt cccgcaacgg ttgaggttgt
      181 cgacatcgcc gggctcgtcc gcggcgcgca ctccggggaa gggttaggca accagttcct
      241 gggccacatc cgtgaagtcg atgcgattgt gcacgtggtg cgttgcttta cagcggcaga
      301 tgtcccgcac gtggaaggcg tggtcgatcc gatgcgcgac attgaaatcg tagaaaccga
      361 actggcattg gcgaatctat caaagcccgt gctttacgtg gccaatatca gcgaggacga
      421 tctccccgcc ggtccttgtg ccgagctggt cgtccggcat gcccaatctc aggaagcgat
      481 cgccttgccg ctttcagtga aactggaagc tgaggttgca gagctcggcg gtcgcgaagg
      541 ggatgagctt ttgagagcct acggattgac tcaacctggt ctgttccgtt tgatccgcgc
      601 agcctacgac ttgctgagcc ttgtcacgtt cttcaccacc gcttcgcggg aagtccgcgc
      661 ctgggcagtt ccatccggca cccgggcgcc agaagccgcc ggaaagatcc acacggacat
      721 gcaacgcggt ttcatcaagg cagaagtcat cggctggcga gagttggtcg aggcaggttc
      781 actgcaggag gctagggatc gagggctcgt acggctcgaa gggaaggagt atgtcgtcgg
      841 cgatggcgac gtgatgctct tcaaattcgc agtgtagcca tcccagaaaa ccggtgatat
      901 aatcgggaag ccccggaagt cagttggggg cttctttacg ttatctggag gtattgcgtg
      961 aagagttacg acctggtctt cgtgatccgc ccggacctgg aacaggatgc gtccaaaacg
     1021 ctcgtagacc gtgtaaccca acgtatcacg gaccagggcg gcacggtcga agcggtggat
     1081 acatggggaa agcgacgcat gtcattctca ctcaagaaga accgggaagg actatttatt
     1141 cacacccgtt tctctttgga tccgtcgaag gttgctgagg ttcgccgcgg tgtgaacctc
     1201 attgaggaag tcctgcggtt cacgctgacg aacgccgtgg gaaagacgcc tgagccgaag
     1261 tccgccgagg tcccggcccc cgcggccccg gttccagtga caccgaccga gccaccggca
     1321 ccaacgaccc cgagcgccgc gggagcgtga cgtgctcaac cgcattgtcc tcatcggccg
     1381 gctgacccga gatcccgaac tccggtatgt tccaagtggc catccggtcg ccagcttcac
     1441 gctggcagtg gaccggccgt tcgtgaatca gcagggcgag cgggggacag atttcatcga
     1501 cgttgtggcc tggcggcggc ttgcggaaca agtaacgcag catttgaata agggccggct
     1561 cgtcgcagtg gagggtcgcc tgcagatccg gtcatatgag acgcaggatg ggcagaagcg
     1621 caaggtcgcc gaggtcgtcg ccgacggcgt gcgtttcctg gaccgtaagg ccgtcggcgc
     1681 aggcgcacca acagagacac cggcgcaaga cggggagccg gagagtgccg aaggcgagga
     1741 cgacgtccca ttttaggcag ttcacggagg cagtatgccc gagggtgatc gcgacagagg
     1801 agatcggaga ggcaagaaag agtggcgggg acgacgcccg aagcggaaga actgtcaatt
     1861 ctgcagtgag aaagcgcagt tcatcgactt caaggatgtc atgcgattgc gcaggttcgt
     1921 aaccgagcgc ggcaagattc ttccccgccg ggtgtccgga aactgcgcga ggcatcagcg
     1981 ggcgcttgcc ggcgctgtga agcgcgcccg ggaactcgcc cttctgccct ataccgctga
     2041 ataacgaaag cgcgcccaca cgaaatcggg gcgcgacggg ttctgctgtg ggaatccgcg
     2101 ggctcaccga aggtgccatt ctcgctacgc tcgtcgctct gcttgcgctg gcggcgcgat
     2161 atctacccgt cattcagttc gcggcagcgt tgatctgtcc gatcccgctc acattccttg
     2221 tgatccgaca gggaatccgc gttgctgtgc tcgcggcgtt ggtcgccgcg gccgtcggcg
     2281 cgatcgccgg cggcccgctg actggtgttg caatcgtcgc gacatttgcg ccaattggga
     2341 tcgcgctggg tctgggtgtc cgcaaccgct gggttcctgg aagaattgtg ctggtgtgca
     2401 ccgcggtcgc aacggcgtcg ttgctcatca acttcgggct caccctgttc atcgcgggtg
     2461 tgaatccgta tacggtgatg ttcgagagcc tgcagcaggg acaggagcag tcgctgcgct
     2521 tgtacgagcg gctcggcatc gatcgtaaga acctcgagca ggcgtcaggt atgatgaagc
     2581 agtggctgga gctcatgccc aggctgatac cgctgttgat tgttggcgga gccgcgtttg
     2641 ttgcactcat taactttcat gtgggacgtc ttgtcctgcg ccggtttggg attgaagtcg
     2701 cggcgctccc tccattgtcg agatggcgga cacacgccct agtcctgtgg accctgccca
     2761 tggggttctt gctcagcgtc ctcggacagg gccgtaatcc ggcggtcgag accgcgggat
     2821 tgaatctaac cctcttcgcg cagacgctgc tattgctgca agggttactc gtcgcctggt
     2881 cgttctttga tcgctacggc tcgccgaatt ggatgcggtg gatcatgatt gccatcgcgt
     2941 tcacgcagcc catcctcaac ctggtgatat ttgtcctcgg actggcagac gccgtgtttg
     3001 atcttcgcgg ccggtggaga ctcgcgaggg aggcacgcac atgaaggtca tcctgctgaa
     3061 aaatgttccg gggctgggtg aacgcgggca gatcaaagac gtcaaggacg gttatgcttt
     3121 taactacctt ctgcctcgcg aactggcggc agaagcaacc cccgggaacg tacggtcact
     3181 tgaaagccag aagaaggctg tcgaagaccg ctcaaaacgg gagcacgagg atactgagca
     3241 gctggcgtcc cagatctcac aggctgtctt ggagatccgg acgcgtgcag gtgagggcgg
     3301 acgtttgttc ggtgctgtga cggctcagaa cgtggcggac gcgctggcgg ggcagggtgt
     3361 gcgggtatct aagagacagg tggagcttga cgagcccatc aaaaccgcgg gattctacaa
     3421 ggtaccggtc cggatcagcc cgggcgtggt agtccgagtc gaggttaacg tagtcgggac
     3481 ggcctaaatg catagagtga acaagatcgt acccgccatc aatccgaccg gatcgctctc
     3541 ggggcgagag acagttcgcc gcaaggtggc tgcgctcttt ggcatcctcg agggaatcca
     3601 cggcgcggag cgcctgcggc ggcgggcaga gaaactcagc gccctcgggc tcatgcgcag
     3661 ccgtcagctc ggccgccggg tgctcgcgct gcagcggctg gtgtttgacg acccacagat
     3721 cacgaccatt ccgcgagaac ctgagatccc ggcgatcctc gaagatctcc aggaacgggt
     3781 cgccgacctg atggcgcgca agtcggttga aacagaactc gaccgtcggg tcgccgaccg
     3841 gatgcgagag cgccaggacg attacctgcg cgagattcgc ctgcaaatgc ttcaggagac
     3901 ggccggacca gagacaccag cgacacgccg caaacttgcg gaactcgagc agctcgagca
     3961 ggtccggctc gcccgtaccg cactggaagc gctgcgcccg aagcgattgg gccacgtggt
     4021 cgggcaggac cgtgccatcc gctcgctgct tgccaagctt gccgttccga tcccccagca
     4081 cgtgattctc tatgggccgc cgggcgtagg taagaccacc gcggcgcgac tcgtcctcga
     4141 ggaagcgaag caactgcggc acacgccgtt tggagaagac gcgccattcg tcgaagtcga
     4201 tggcagcacc ctgcgctggg atcatcggga ggcgacgaac cccctgctgg gctcggtgca
     4261 tgacccgatc tatcagggta cgcggcgtga tttcgccgac gccggcatcc ccgagccaaa
     4321 gctggggctc gtgacgaagg cgcacggcgg cgtcctgttc atcgacgaga ttggagaact
     4381 cgaccctgtg ctgcaggcaa aattactgaa ggtgctggaa gacaaacgag tacgcttcga
     4441 gtccagctac tacgatcgcg acaatccgca tctccccgcc tatgtgcgcc ggctgtttga
     4501 tgaaggcgcg ccggcggact tcgtcttgat cggcgccacc accaaggagc ccgaggacat
     4561 cagcccgacg ctgcgatcgc gatgcgccga agtgttcttc gatccgctgt cacctgaaga
     4621 catccagcgg attgtccgcc aggccggccg tcgcctccag gtggaagtcg gcacggacgt
     4681 gcccaaaact atcagtgact acacaattga aggacgcaag tcggtgagca tgctcgccga
     4741 cgcgtacggc gccggtctct atcgccggcg gggcagcggt gggttgcgaa tcacgctgcg
     4801 tgacctgcac gacgtgatcc gatccggccg gttgtcggcc agcgcgccgg cgaaggcttc
     4861 cgccgtccct gaaatcggca gagtgtttgc gctcggcgtg acacagtatg tcggctcgat
     4921 tatcgagatc gaagccacgg cgtttccggt gggcaagcgc cagcggggca cgatcaggtt
     4981 caatgaaacg gcgggctcga tggcaaagga ctcggtcttc aacgcggctt cagtgatccg
     5041 ccggctcacc ggtatcgatg tcagccactt cg
//
LOCUS       DBNGSB010000101         3743 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_1296264, whole genome shotgun sequence.
ACCESSION   DBNGSB010000101 DBNGSB010000000
VERSION     DBNGSB010000101.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3743)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_1296264"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="AC159B_02790"
     CDS             complement(<1..450)
                     /locus_tag="AC159B_02790"
                     /inference="COORDINATES: protein motif:HMM:NF015901.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HIR3376711.1"
                     /translation="MKKVSWIVLSCLVLGLSSRAAGQGFQEPTADQGRLQADRVRYDA
                     KKKIFTAEGNVHLVMGDVEVHAKRARVEQIPQRVTASGDVLVVQRETSLRAAEVIYDM
                     RPGIVNASGDVFVAWRDLLVNAPALKYGVRTEQVAARGGVKLAQGNSI"
     gene            complement(567..1136)
                     /gene="lptC"
                     /locus_tag="AC159B_02795"
     CDS             complement(567..1136)
                     /gene="lptC"
                     /locus_tag="AC159B_02795"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HIR3376712.1"
                     /translation="MRRFKVVVSLLVALAIGIVVGRLWRQPSTSPPTQTQQAQPAQSP
                     AIGLRQASIVLRHKGTKQAEVQAASVAVSRDLRYATFKRIEKVTLYDQGVASLTLRTG
                     EIILDRTTNDLVARGEVEISSPQGDQLWAPEARWDNALQRLVFPQGVRLKVGGAEVSA
                     SRLNVDTRLQVVDLEGGVDITFQLPANRP"
     gene            complement(1123..2250)
                     /locus_tag="AC159B_02800"
     CDS             complement(1123..2250)
                     /locus_tag="AC159B_02800"
                     /inference="COORDINATES: protein motif:HMM:NF015684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HIR3376713.1"
                     /translation="MRVPIVDRYILKELLGPFLLGIAVFIVILIGDILYTLAEFIASG
                     RVTVDTILKLLAYKMPAVMVITFPVSTLIGTLLGLGKLAKNREVQAMRLFGMSLLRIF
                     VPVLAFGLAVAGLTLVTNEFVAPWANHKANNLIRRAAFAEAFTQVRTQVFFRGPGQRF
                     FYVDSVDDNQRVLHNVMIYEAAGPLPRLITARRATLHEQKWSLAGGVIREFDEAGFTR
                     YEAQFASMELAVGVDSGMFFAGQKTPEEMTAKELRRYLALFSSGGAAALRFAVEYHRK
                     FAVPFAAAVFALVAAPLSVQTEQGGRFLGAGLGVVLLFIYYVAMSISRAMGGVGALSP
                     VLSAWLPNVLFAVCGILLWVREEGGLRRAPVRRVVTVGAPL"
     gene            2293..3743
                     /locus_tag="AC159B_02805"
                     /pseudo
     CDS             2293..3743
                     /locus_tag="AC159B_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922046.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
BASE COUNT          720 a         1199 c         1174 g          650 t
ORIGIN      
        1 gatactgttt ccttgcgcga gtttgacccc gccccgcgcg gcgacctgtt ccgtgcggac
       61 gccgtattta agcgcggggg cgttgacgag caagtcccgc caggcgacga atacgtcgcc
      121 cgacgcattg acgatgccgg ggcgcatgtc gtaaatgacc tctgccgcgc gaagcgatgt
      181 ctcgcgctgc accacgagca cgtcgcccga tgccgtgacg cgctgaggaa tctgctcgac
      241 ccgcgcccgc ttcgcatgca cctccacgtc gcccatgacg aggtgcacgt tgccctcggc
      301 cgtgaagatc ttcttcttgg cgtcataccg cacacggtcg gcctgaagcc tgccctgatc
      361 cgcggtgggt tcctgaaatc cttgaccggc cgctcttgag ctcagcccca acaccaggca
      421 ggacagtacg atccagctca cttttttcat tactctacgc gtcctatcca agggcagcag
      481 gtggcggagt gaagccagag gggtccccgt atcagttgca gtcggggaga gggttcgctc
      541 cgccacctcg ctgcccttac ttcctctcaa ggtctgttag ccggcaactg gaacgtgatg
      601 tccacgccgc cctccaagtc gacgacttgc agacgagtat cgacgtttag gcgggaggcg
      661 ctgacttcgg ccccgccgac tttcaagcgt acgccctgcg gaaagacgag ccgctgcagg
      721 gcgttgtccc atcgcgcctc gggtgcccac agctggtcgc cctggggtga agagatctcg
      781 acctcgccgc gcgcgacgag gtcatttgtc gttcggtcga ggatgatttc gcccgtccgc
      841 agtgtcaacg aggcgacgcc ctggtcatag agcgtcacct tctcgatccg cttgaaggtt
      901 gcatatcgga ggtcacggct cacggcaaca gatgcggcct gcacctccgc ctgtttcgta
      961 cccttgtggc gtaggacgat gctggcctga cggagaccga ttgcgggcga ctgggcaggc
     1021 tgcgcctgct gggtctgagt cggaggagag gtagatggtt ggcgccacaa tcgccctacg
     1081 acaatgccaa tggcaagggc gacaagcagt gaaacgacga ccttaaagcg gcgcaccgac
     1141 cgtcaccacc ctgcgaacgg gggcccgccg cagcccccct tcctcgcgca cccacaacaa
     1201 gatcccgcac accgcaaaca gcacgttggg cagccaggcg ctcagaacgg gagacagagc
     1261 cccaaccccg cccatggccc gtgagatcga catggctaca tagtagatga ataacagcac
     1321 cacgcccaac cccgctccga ggaagcgccc tccctgttct gtttggacgc tgagcggggc
     1381 ggcgacaagg gcgaagaccg cagccgcgaa cgggacggcg aacttgcggt ggtattcaac
     1441 ggcgaagcgc agggccgcgg cgccgccgct gctgaagagc gcgaggtagc gtctcaactc
     1501 tttggccgtc atttcctcgg gggttttctg tcccgcgaag aacatcccgc tgtcaacgcc
     1561 cacggcaagc tccatggagg caaactgggc ctcatagcgc gtgaaccccg cctcatcaaa
     1621 ctcacgaatc acgccgccgg cgagtgacca cttctgctcg tgcagggtgg cccgccgcgc
     1681 cgtgatcagc cgcggaagcg gtcccgcggc ctcataaatc attacgttat gcaggacgcg
     1741 ttggttgtcg tccacgctgt ctacatagaa gaagcgctgc cccggaccgc ggaaaaacac
     1801 ctgcgtacgg acctgggtga aggcctcggc aaacgcggcg cgccggatga ggttgttggc
     1861 cttgtggttg gcccacggcg cgacaaactc gttggtgacc agggtgagcc cggccaccgc
     1921 caggccgaag gccaggacgg gaacaaagat cctcagcagg ctcatgccga agagccgcat
     1981 cgcctggact tcgcggttct tcgccagctt cccgagtccc agcagggtgc cgatcagcgt
     2041 ggacaccgga aaggtaatga ccatgacggc gggcatcttg taggccagca gcttgaggat
     2101 cgtgtcgacc gtgacgcgcc ctgacgcgat gaactccgcg agcgtgtaca aaatgtcccc
     2161 gatgaggatc acgataaaca ccgcgatccc gagcagaaac ggccccagga gttccttgag
     2221 aatgtaccga tcgacgatgg gaacccgcat gtacattgcc tgttctatat ccctgtgata
     2281 gaatcccgca caatgccgac gttcaggccg gtggacacca aggcagactt tcccgcgatt
     2341 gagcgtgagc tgctccgctg gtggcgcgaa gagcgcatcg tggagcgcta cctgcagcgg
     2401 aacgagcgtt cgccccgccg ctggtcgttc atggacggtc ccatcacggc caacaacccc
     2461 atgggggtcc accatgcgtg ggggcggacc tacaaggatc tcttccagcg gtacaagacg
     2521 atgcgcgggt tccgccagcg ctaccagaac ggcttcgact gccagggact ctgggtcgag
     2581 gtcgaggtcg aaagagacct cgggttcaca aacaagcgcg acatcgagac gtttggcatc
     2641 gccccgttcg tcaacctctg caaggagcgc gtcctgacct acgctgctgt gcagaccgcc
     2701 cagtccatcc ggctcggcat gtggatggac tggaatgatc cggacgatct gcgcgcgctc
     2761 gcggacgcgg tcaagtctga cccggcgcag attcggacct atcccgcaag gggcggcgca
     2821 atccgcggga gcgcggaaca ggtcgtgggc cggctgggcg attcggagac cagcggctcg
     2881 tactttacgt tctccaatga gaacaactat caaatctggg cattcctcaa acgctgctgg
     2941 gagcggggga tgatctacaa agggcaccac gtgatgccct ggtgtccacg ctgctccacc
     3001 ggcatcagcg agcacgagat cgtcaccgaa ggctatgagg aacgcacaca cccggggctg
     3061 ttcgtccgct tttcacttgt cgagcagccc aagcggtcgc tcttggtgtg gaccacgacg
     3121 ccctggacgc tgacgagcaa cgtcgccgcg gccgtgcatc cagacctgac ttatgcgctc
     3181 gttcggcagg gaaatgagga gttgtacgtc gagcagggag ccctcggtgt gctgcgcggc
     3241 gcgtacacca tcgtcgaaca gatgcctggc gagcgtctgg tcggcctccg gtaccgcgga
     3301 ccatttgacg acctgcccgc gcagcgcggc gtcgagcacc gggtcatccc ctggaaggac
     3361 gtaagcagcg cggagggcac gggcatcgtg cacattgcgc cggggtgcgg cgcagaggac
     3421 ttcaagctcg ggaaagagca tggcctgccg gtcatcgcac cgatcgatga atacggcgtg
     3481 ttcgtgaccg gattcgaccg gctcagcggt ctgcgtccgc acgcggctgc cggcccgatc
     3541 accgagcatc tgcgaaacag cgccgtcttt acaaggctga atcgtacacg ccccgctacc
     3601 cggtgtgctg gcggtgcggg agcgagctcg tcttccgtct ggtagacgag tggttcatcg
     3661 caatggatcc gatccgcaag gatctgatgg agatcacgcg tcggatccgg tggatccccc
     3721 gattcggact cgatcgcgag ctc
//
LOCUS       DBNGSB010000102         3332 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3596775, whole genome shotgun sequence.
ACCESSION   DBNGSB010000102 DBNGSB010000000
VERSION     DBNGSB010000102.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3596775"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /locus_tag="AC159B_02810"
     CDS             complement(<1..1020)
                     /locus_tag="AC159B_02810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3376714.1"
                     /translation="MRLALIADGRAEHVRRWMRYLTDAGDELLILSTHRCLPMSGVNM
                     AVLPGVFRTRGLLVTQPEARPNVNRAAVAVASLGLGKGAQYVWQQVKTVDVTSQGPAA
                     RKLLAEFRPDLVHAFRLQTEGYVAALAGWRPFVISTWGSDFIWFARKYPVHRFLTRSA
                     LRRADALLPDCERDLELARDAGFRPDRPSMVCPGNGGVDFEVYRPGQDARTRDPLIVY
                     PRGLTPSVRLDTLIRALLLLKDSPESIMPEAILLVQSAFVPVVTRMVERAGLSVDRVR
                     VMPMVGQEGWVSLLQRAAVMVSPSLSDGTPNSMLEAMACGTFPVIGDLASIREWICHG
                     VNGLLF"
     gene            complement(998..2185)
                     /locus_tag="AC159B_02815"
     CDS             complement(998..2185)
                     /locus_tag="AC159B_02815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3376715.1"
                     /translation="MRSYRAILLTNILSPYRIPLFNALKACEGWTFTIAFLAERESNR
                     RWRVRADELTVKHVTLPGWSRYVWRLELPLHVNWGVYRFLKREDPDAVILGGYASLGS
                     WLALLFCRLNGRRLILWSGTTHASVRMRSRLMQAARRFFVRAAGAFVTYGTRAAEFLV
                     SLGARRESIFVGPNVGDVEFFREAVRRFRETPDFAQGRATLRGPVILFVGQFIPRKGI
                     VQLLRALSEVRGRDWTAYVVGDGPLRRHLESLRVELGLSGRVHFKPFQEREELVTFYG
                     LADILVLPSLLEVGAIVVSEALASGLFVLASQGDGVAPDLIQPGRNGHIFDPSNPRAF
                     SSAIEDALQIVSRTNVRNDIVDSMASHTPEQYALAFCRALRHALASVRAHQEPADAIG
                     ADR"
     gene            complement(2187..>3332)
                     /locus_tag="AC159B_02820"
     CDS             complement(2187..>3332)
                     /locus_tag="AC159B_02820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3376716.1"
                     /translation="SSGVLTSKQAVVEYAHGLAGWFRQTTWATTLYPHTAHTRTPVDL
                     ERVRPSVVRGDRGGLLSDFRFLRRRTSGETVLLLHLPNPWLTLSVLVCRLRARGMFVY
                     VASDYAGYSAAAARTRGLVYARVYRFLYEMAIRSANGVIVRGAANEERARRLNRNVIV
                     SLPLALRTVSRPRDEPACAGPTIRVLYVGKLTLDKGVEVLVQAFEECVRQLSPRRDAT
                     LTFIGQGSAALRLREMSDGLGLGAAISLRGFVDDLEELSASYAEADLVVVPAISPEGV
                     PRVIDEALMHGTPVVASAVGGIPAEFSGGEVALVPPGDAGRLAAAMLRVVLDEPFRSE
                     LHAAASARVRRLAAQPSAADQHAQFIASTLGFPVASGAVSGIAQAEA"
BASE COUNT          677 a         1123 c         1024 g          508 t
ORIGIN      
        1 gaagagcagc ccgttcacgc catgacagat ccactcacgg atagatgcta gatcgcctat
       61 caccggaaag gtaccgcagg ccattgcctc cagcatcgag ttgggcgtgc cgtcactcaa
      121 cgatggcgag accatcacgg cggcgcgctg cagcagtgag acccaccctt cctgacctac
      181 catcggcatg acgcgcacac ggtcaaccga aagtcccgcc ctctcaacca tcctggtaac
      241 aaccgggacg aacgctgact gtaccaacag gatggcctcc ggcatgatgg attcgggaga
      301 atctttgagc agtaagagcg cgcgaatgag cgtgtcgagc cgaaccgacg gggtcaaccc
      361 gcgcgggtac acgatcagcg gatcccgagt gcgcgcgtct tggccggggc gatacacctc
      421 gaaatcaaca ccgccgtttc ccgggcacac catcgacggg cgatccgggc gaaacccggc
      481 gtcgcgcgca agctcgagat cgcgctcaca gtcgggcagc aaggcgtcgg ctcgcctgag
      541 ggccgagcgc gtcaggaacc ggtgcacagg gtacttccgc gcaaaccaga tgaagtccga
      601 gccccacgtg gatatcacga agggcctcca cccggccagg gccgcgacgt agccttcagt
      661 ctgcaggcgg aaggcgtgca ccagatcggg ccggaactcg gcaagcagtt tcctggccgc
      721 cggcccctgg cttgtgacgt cgacggtctt gacctgctgc cagacatact gcgctccctt
      781 tcccagcccg agcgatgcta ccgcgaccgc cgcgcgattc acgttcggac gtgcctccgg
      841 ctgtgtgacc agcagccccc gcgtgcgaaa tactcccggt agcacggcca tgttgacgcc
      901 ggacatggga agacaccgat gcgtgctcaa gatcaacagc tcatccccag catctgtgag
      961 gtatcgcatc cagcgccgca cgtgttcggc gcggccatca gcgatcagcg ccaatcgcat
     1021 cggcgggctc ttggtgcgcg cgcacagacg cgagcgcgtg tcgcagtgcg cgacaaaagg
     1081 cgagcgcata ttgttcggga gtgtgggatg ccattgagtc cacaatgtca ttccgcacat
     1141 tcgtccgtga cacgatctgg agcgcgtcct cgatcgcgga cgaaaacgcc ctggggttcg
     1201 acgggtcgaa gatgtgcccg ttgcgaccgg gctgaatgag atcgggcgcc acgccgtcgc
     1261 cctgcgaggc gaggacaaac aacccgctgg ccagcgcttc gctgaccacg atggcgccga
     1321 cctcgaggag cgacgggagg acgagaatgt ctgcgagacc gtagaacgtc acgagttcct
     1381 ctcgttcctg aaatggtttg aagtggactc tgccgctaag tcccagttcg acgcgcagtg
     1441 actcgagatg gcggcgcagc ggtccgtcac cgacgacata ggccgtccaa tcccgtccgc
     1501 ggacctccga aagcgcgcgt aacaactgga cgataccctt tcgcggaatg aactggccga
     1561 caaacaggat caccggaccg cggagcgtcg cgcgaccttg cgcgaagtca ggcgtctcgc
     1621 ggaagcgccg aaccgcctcc cggaagaact ccacgtctcc cacgttggga cccacaaaaa
     1681 tcgactcgcg cctcgcgcca agcgacacaa gaaactctgc cgcgcgggtg ccgtaggtga
     1741 caaaggcacc ggccgccctc acgaagaacc ttcgtgccgc ctgcatcagc cggctgcgca
     1801 tccggacgct cgcgtgtgtc gtgcccgacc acagaataag acggcggccg ttgagccggc
     1861 agaacagcag ggccagccag ctgccgagag aggcatagcc gccgaggatg acggcatccg
     1921 gatcttcccg ctttagaaac cgatacacgc cccagttcac gtgcagcggc aactcgagac
     1981 gccacacata ccggctccaa ccgggcaggg tcacatgctt cacggtgagc tcgtcggcgc
     2041 gaacgcgcca gcggcggttg gactctcttt ctgcgaggaa tgcgatggta aacgtccacc
     2101 cctcgcaggc tttgagcgcg ttgaacagcg ggatccggta tggagacagg atgttggtca
     2161 ggaggatagc tcgatacgac cgcacctcag gcctctgcct gcgcgatccc cgacactgct
     2221 cctgacgcca ccggaaagcc gagagtagag gcgatgaatt gcgcatgctg atctgcggca
     2281 gacggctgtg ccgcgagccg gcgcacccgg gcgcttgcgg cggcgtgcaa ctccgacctg
     2341 aacggctcgt cgagcacgac gcgcagcatt gcggccgcga gcctcccggc gtcaccgggc
     2401 ggcacgagcg cgacctcacc tcccgaaaat tccgccggga tcccgcccac ggcggacgcg
     2461 acgacgggcg ttccgtgcat cagcgcctcg tcgatgaccc gtgggacccc ctccggtgat
     2521 atcgccggga cgaccacgag atcagcctcc gcgtaagagg cgctgagctc ttccagatcg
     2581 tcaacaaatc cgcgcaaact gatcgccgcc ccaagaccga gcccatcgct catttcacgc
     2641 aaccgcaatg ctgcggaccc ttgcccaatg aatgtcagag ttgcgtcccg ccgtggtgag
     2701 agttgccgga cacactcttc aaacgcctgc acgagaacct cgacgccctt gtcaagcgtg
     2761 agctttccta catacaagac gcggatcgtt gggccggcac aggccggttc atcccgcgga
     2821 cgcgatacgg tgcgaagagc cagaggcagc gacacgatga cgttgcgatt cagccggcgc
     2881 gcccgctcct cgttggccgc gccccggacg atgaccccat tggcgctgcg aatcgccatc
     2941 tcgtacagga agcggtacac gcgtgcgtag accaacccgc gcgtacgtgc cgcggccgcg
     3001 ctgtacccag catagtcgct tgccacatag acgaacatgc cccgcgctct caaccggcac
     3061 accagcaccg agagggtaag ccagggattg gggagatgga gcaggagcac ggtctcgccg
     3121 ctggtgcgtc gccgcaggaa tcgaaagtcc gacaacaacc cgccgcgatc acctcgcaca
     3181 acggacggcc tgacccgctc gaggtccacc ggcgtccggg tgtgcgcagt gtgcggatat
     3241 aatgtcgtcg cccaggtcgt ctgcctgaac cacccggcca gtccgtgggc atactcgacc
     3301 actgcctgtt ttgaggtcag cacgccggag ga
//
LOCUS       DBNGSB010000103         4021 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2984526, whole genome shotgun sequence.
ACCESSION   DBNGSB010000103 DBNGSB010000000
VERSION     DBNGSB010000103.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4021)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2984526"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="AC159B_02825"
     CDS             complement(<1..240)
                     /locus_tag="AC159B_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HIR3376717.1"
                     /translation="MTFLAASTAPVPREGKGAVAIIGVPFDGTSTFRRGSRHGPAAIR
                     WASQSIETYSPVLQRELENVELFDGGDLDIAGAGVD"
     gene            complement(237..1067)
                     /gene="speE"
                     /locus_tag="AC159B_02830"
     CDS             complement(237..1067)
                     /gene="speE"
                     /locus_tag="AC159B_02830"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002010.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HIR3376718.1"
                     /translation="MTEWISDIVGPGYAQSIQVDTILYDGRSAFQHIQVATSPTFGRM
                     LILDNAVQTTEADEFIYHEMLVHPALVSHPRPRRVMIIGGGDGGTLEEVLKHQVEHVT
                     MVEIDREVVEVSRAFLPSICGRAFDDRRVHLSIDDGVAFIQRTHEKFDVILVDSTDPK
                     GPGVALFSEAFYAACARALTDEGLVVAQSGSYFFQRELTTLVRERLGSVFPLTMTFWA
                     AVPAYPGVLWSFSCGSRRHDPRALPGERIAERLRSFSTRYYRPQMHAVAFTLPPNVGA
                     "
     gene            complement(1192..1650)
                     /locus_tag="AC159B_02835"
     CDS             complement(1192..1650)
                     /locus_tag="AC159B_02835"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - L-arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvoyl-dependent arginine decarboxylase"
                     /protein_id="HIR3376719.1"
                     /translation="MWERPSSVSVVAGSGEGRTELTAFDRALMDAGIANLNFIRITSI
                     LPAGVRIVPVPSFVAGGLTPVVYARIASHMPGERIAAAVGLGLSKNSFGVIMEYEHPG
                     TAEVAEAIVRGMVEEAMAVRDLPIDNIVVAAKDHVVERIGCAVAAVVFWP"
     gene            1757..3400
                     /locus_tag="AC159B_02840"
     CDS             1757..3400
                     /locus_tag="AC159B_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376720.1"
                     /translation="MPYRFVALATVAALLVGALPTGAALAPQPSLPVGFVTAGPADFA
                     SMRAAGATTVKVIADWSAIEREKGKFVWEVLDQSVTAAERAGLSVVIVLSYTPKWASL
                     ATGIELNDPMIFSRQPPKRIADWEGFVTAIVTKYHARVKNWQVWTALSLPLFRGTDRE
                     YLALLRATRAKARAVDPSSRIVLATPFGLDLISIRKAILEAGDAFDIISLSPRALAPE
                     SLMRPLGVLTQRVLGRASKRIWIEWDPRSVGERATWPAQTIKLMAIARAFAVEHLFWA
                     AEPAPGMTAAVSTFTAEVASRPFAGFVMVDRGIAFLFGDGEPAAAVAWSTTGESSFKF
                     DGNLKALSPTGDAKALGSIGTEPVLVAGIGATSVAEAKSTLQARGLPVPPLQDDFSQQ
                     TEVSAKLGRTNVERGLYNAPYRSRRNGAVEPIEVSGSDAVRTSNARDIVFIYFDVDDS
                     FLFYVDGRSAVEISIEVHGASAPDQLGFNVLYDSMTGYRFTSWHVVEAKDGWVTQTIR
                     LNDAGFSNTWGWDFAINAGGNRKEDLTVRSVTVKKVARP"
     gene            3536..3883
                     /locus_tag="AC159B_02845"
     CDS             3536..3883
                     /locus_tag="AC159B_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012620274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HIR3376721.1"
                     /translation="MIDDCIFCRIVSGKIPAQRVYEDEAVLAIRDINPQAPVHLLVLP
                     KKHLASVQDASKQDAPLIGALTLAAVQLARTEGLDHRGFRLVINVGPEGGQTVPHLHI
                     HLLGGRQMKWPPG"
BASE COUNT          794 a         1226 c         1247 g          754 t
ORIGIN      
        1 gtcgacgccg gcgccggcga tgtccagatc gccgccgtcg aatagctcga cgttctcgag
       61 ttccctttgc agcaccgggc tgtaggtttc gatgctttgt gacgcccacc ggatcgccgc
      121 gggtccgtga cggctcccgc ggcggaaggt tgaagtgcca tcgaacggca cgccgatgat
      181 ggcgacggcc cccttaccct cgcgcggaac gggggcggtg ctggcagcga gaaaggtcat
      241 gccccgacgt ttggcggtag ggtgaacgcc acggcgtgca tctgcggccg gtaatagcgc
      301 gtggagaacg accgcagccg ttcggcaatc cgctccccgg gcagcgcccg cggatcgtgg
      361 cgcctgcttc cgcaggaaaa actccagagc actccggggt acgcagggac cgccgcccag
      421 aatgtcatcg tcagtggaaa cacggaaccc agacgctcgc ggacgagagt ggtcagctcc
      481 cgctggaaaa aatacgatcc gctctgagcg acaaccagcc cctcatccgt cagcgcgcgg
      541 gcgcaggctg cgtagaatgc ctccgagaag agcgcgacgc cgggaccctt aggatcggtc
      601 gaatccacga ggatcacgtc gaacttctcg tgcgtgcgct ggataaacgc gacaccgtcg
      661 tcgatcgaca gatggacgcg acggtcgtca aaagcccgtc cgcagatcga aggcaggaac
      721 gcgcggctga cctcgaccac ctctcgatcg atctccacca tcgtcacgtg ctcgacctga
      781 tgcttgagca cttcttccag cgtaccgccg tcgccaccgc cgatgatcat cacgcgacgc
      841 gggcgagggt gactgaccag agccgggtgg acgagcattt catggtagat gaactcgtcg
      901 gcctctgttg tctggaccgc attgtcgagg atgagcatgc ggccgaatgt tggactcgta
      961 gcgacctgga tgtgttgaaa cgccgagcgg ccatcgtata ggatcgtgtc cacctggatg
     1021 gactgcgcat agcccgggcc gacgatatcc gaaatccact cggtcaaggc acccctccaa
     1081 ctacgcgaac aaagcgaact aggttaacat ggcgaactag gtcaaacctg agtgtgctct
     1141 tcataacctt gttcgcttcg ttcgctttgt tcgcatgttc gcgattttgg gtcagggcca
     1201 gaacacgact gccgccactg cacagccgat tcgctccacg acgtgatctt ttgcagccac
     1261 gacgatgttg tcgatgggaa ggtcgcggac tgccatcgct tcctcaacca taccgcgcac
     1321 gatggcctcg gcgacctcgg ccgtgcccgg atgctcgtat tccatgatca ctccgaaact
     1381 attcttcgag agaccgagcc cgaccgcggc cgcgatgcgc tcaccgggca tgtgactcgc
     1441 gatgcgcgcg tacaccacgg gcgtcagccc gcccgccacg aatgacggca cgggcacgat
     1501 ccggaccccc gccggcagga tgctggtgat ccgaatgaag ttaaggttcg caatccccgc
     1561 atccatgagt gcgcgatcga aggctgtgag ctcagtgcgg ccttctccac tcccggcgac
     1621 gacagaaacc gaagagggtc gttcccacat gcggttggat attcaattgg agaactcctt
     1681 aacctccgag actcgtactc gcacgtgcag ggtaaaatac gtggcggatc gcaaccaaac
     1741 aacaaggagc ttcgacatgc catatcgatt cgtcgcactc gcaaccgtcg ctgcactgct
     1801 cgttggtgcg cttcctaccg gcgctgcgct cgcgccacag ccatcattgc ccgtggggtt
     1861 cgtcacggcc gggcccgccg actttgcgag catgcgtgcc gccggagcca ctaccgtcaa
     1921 ggtgatcgcc gactggtcgg ccattgagag agagaaagga aagtttgtct gggaagtcct
     1981 cgatcagtcc gtgaccgccg cagagcgcgc cggactgagc gtggtgatcg tactatcgta
     2041 cactcccaag tgggcatcgc tggcaacagg catcgagttg aacgatccaa tgatcttcag
     2101 ccgccagcct ccaaagcgca tcgctgactg ggaaggtttc gtgaccgcca tcgtgaccaa
     2161 atatcacgcc cgggtaaaga actggcaggt ctggacggct ttgagtctcc cgctgttccg
     2221 cggcaccgac cgggaatacc tcgcgctgct gcgggccacg cgtgcgaagg caagggccgt
     2281 ggatccttcc agccgcatcg tcttggcaac gccattcggt ctggacctga tctcgattcg
     2341 gaaagcgata ctcgaggcag gggacgcgtt tgacattatt tctctgtctc cccgggcgct
     2401 cgcgcccgaa tcgctaatgc gtccgctggg ggtgctgaca cagcgcgtcc tgggacgagc
     2461 ctcgaaacgg atctggattg agtgggatcc acggtcggtg ggagagcgtg ccacctggcc
     2521 tgcgcagacc atcaaattga tggcgatcgc cagagccttc gccgtggagc acctgttctg
     2581 ggccgctgag ccggcgccag ggatgacggc cgcagtcagt acgttcaccg ccgaagtggc
     2641 cagccgtccg ttcgcaggct ttgtgatggt cgatcgtggc attgctttcc ttttcgggga
     2701 tggcgaaccg gccgcggctg tggcgtggag cacgaccggc gaatcgtcgt tcaagtttga
     2761 tggaaacctc aaagcgctgt cgccgaccgg ggatgcaaag gcgctcggga gtatcggaac
     2821 ggagccggtc ctcgttgcgg gcatcggcgc gacgtcggtg gccgaggcga aaagcacact
     2881 acaggcccgc ggcctacccg tgccgccttt gcaggatgac ttcagccagc agactgaggt
     2941 ctcggcaaaa ctcggccgca cgaacgtcga gcggggactc tacaatgccc cgtaccgcag
     3001 ccggcggaac ggggccgtcg aaccgatcga ggtcagcggg tcggacgcag tgcggacgag
     3061 caacgcaagg gatattgtgt tcatctattt tgatgtagac gatagcttcc tgttctacgt
     3121 ggacggccga tcagcggtgg agatcagcat cgaagtgcac ggagcgtcgg ccccggacca
     3181 gctcgggttc aacgtgcttt atgactcgat gaccggctac cgtttcacat cctggcatgt
     3241 cgtagaggcc aaggatgggt gggtgacgca gaccatccgc ctcaacgatg cgggtttctc
     3301 gaacacctgg ggatgggatt tcgcaatcaa tgcgggtggc aaccggaagg aagatcttac
     3361 cgtccgcagt gtgacggtca agaaagtcgc aaggccgtga gccagctttc ggggactccg
     3421 aggcctctcc ccgaaaagaa actaccacgg ggacccctct agcctcggag cccccggcgc
     3481 tggcttgatt tcatcgcaag cgcgcgccac gctattttgg gggtgaactc agcagatgat
     3541 agacgactgc attttctgca ggattgtttc tgggaagatt cctgcccaac gcgtgtatga
     3601 ggacgaagcg gtcctggcaa tccgtgacat caaccctcaa gcgcctgtgc atctcctggt
     3661 gctcccgaag aagcaccttg cttcagtcca ggatgcttcg aagcaggatg cgccgctcat
     3721 cggagcgctc accctcgcgg cagttcaact cgccagaacg gaaggactcg accaccgcgg
     3781 tttccgtctg gtcatcaacg tcggccccga gggcgggcag acggtgccgc atctccacat
     3841 acacctgctt ggcggacggc agatgaagtg gccgcccggc tgacgcaaac tgttcccttg
     3901 tttcctgact tcggggcctt atagtataat gtttcaagtt tgcaccacgc tcaggcaggc
     3961 ggggggtgtt gagatgactg aagtgagggt cggcaaggac gagtcattgg acagtgctct
     4021 g
//
LOCUS       DBNGSB010000104         5247 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3137731, whole genome shotgun sequence.
ACCESSION   DBNGSB010000104 DBNGSB010000000
VERSION     DBNGSB010000104.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 5247)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5247)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5247
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3137731"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="AC159B_02850"
     CDS             complement(<1..783)
                     /locus_tag="AC159B_02850"
                     /inference="COORDINATES: protein motif:HMM:NF012784.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HIR3376722.1"
                     /translation="MKTSEDQPQLEQIPNLSPRQIVQGTVVRIDTEGVLVDVGAKSEG
                     LIPPNELGKQGEPEVSVGERIDVMVMRVEGDEGNLILSKRRADFEVAWRRVLEAKESG
                     AVLHAMVVDKVKGGLVVDLGMRGFVPGSHVDLTQAKGRRFEWFVGQSVPLKVIEVDRP
                     KGRVILSHRQAVEEERHKRRADLLESLQEGQIVEGSVKRLTDFGAFVDLGGMDGLLPI
                     SEIAWTYIKHPSEVIRRNQRLRLLVLRVERENGRISLGLKQIL"
     gene            complement(853..1296)
                     /locus_tag="AC159B_02855"
     CDS             complement(853..1296)
                     /locus_tag="AC159B_02855"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011530823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR3376723.1"
                     /translation="MPADRAPFGNGRRIVDNTFRVRYAETDAMGVVHHASYLVWFEVG
                     RTEYTRAMGLPYREVEQDGVRLVVIEAHAEFKRAARYDDLIVVRTTVGEVSKATLTFR
                     YEVRLQSDDTLLVSGYTVHAATDLAGRVQRMPERVRTVLMGAAPA"
     gene            complement(1444..2418)
                     /locus_tag="AC159B_02860"
                     /pseudo
     CDS             complement(1444..2418)
                     /locus_tag="AC159B_02860"
                     /inference="COORDINATES: protein motif:HMM:NF013046.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000118 - histone deacetylase complex
                     [Evidence IEA];
                     GO_function: GO:0004407 - histone deacetylase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
     gene            complement(2487..3188)
                     /gene="rpe"
                     /locus_tag="AC159B_02865"
     CDS             complement(2487..3188)
                     /gene="rpe"
                     /locus_tag="AC159B_02865"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017437129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR3376724.1"
                     /translation="MTAESAVTIAASILSADFTRLGEEIAAAERGGVDTIHVDVMDGR
                     FVPEITIGSVIVEAAKRATRRPIDVHLMIMDPERHVPTFARAGAASMTVHVEAVPHLH
                     RVVQQIKDLGVRAAAALNPGTPLGAVEPVLSDLDMVLLMTVNPGYAGQQFIPSVLSKV
                     GQLRSWIEERGLDLDVQVDGGINESTAPRAVDAGANVLVAASAIFKGVGGPEAAARRL
                     KAAANATAKAEGRRQ"
     gene            complement(3185..5092)
                     /locus_tag="AC159B_02870"
     CDS             complement(3185..5092)
                     /locus_tag="AC159B_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3376725.1"
                     /translation="MITQGRVIAGEYEILSRISEGGMATVYSVRRTSDGQVLALKVLR
                     EQYAADAEFVERFQREAKAVSELSHPHMVRVFASGEDNGIHYIAMEFVEGEDLKRLLR
                     RERRLPWSRAAEIAAQVCEALEYAHAHGIIHRDIKPQNILLTKGGEVKVADFGIARAL
                     SSATITQTGTVLGSVQYLAPEQARGLPVGRTADVYALGVVLYEMVTGALPFDEDSPIA
                     IALKHMDQDPPMPSTHVADLPPRGEGIILRALAKAPRYRYQSADQMRWDLLGKTDLWK
                     DLPPPKRTKEDTPATMVLRPGATQELDERRLLVPGPVLALGLIALVIGGVWGGWRAFS
                     SYIAVPEVEVPNFVGKTVQAGEQAASERRLTLRVTEEAFSTDVAAGLIISQDQPAGKP
                     VKVGRVVNVAVSLGPEMVGVPDVQRRALIEARLIIDRARLQIGELREAFDEDVKGGFV
                     ISQNPQPGARLERGSPIHLVVSKGPQRVQMPLLVGRSLLEARRMLQELGVTLAAVKTV
                     PTADMNPETVVDQSPSPGTRIRSQDAVTVTVTVRPGQETIPPPSPVVTAQPQRPPSPE
                     EKLTRVQLVVPGGDPEQDVKIVVIDERGVQTVFQKTLAPGDRVDERIRSRGFTIIQVF
                     VQNQLVQEVRP"
BASE COUNT          927 a         1744 c         1571 g         1005 t
ORIGIN      
        1 gaggatttgc ttcaatccga gagagatgcg gccgttctcg cgctcgacgc gcagcaccag
       61 cagccgcagc cgctggttgc gccggatgac ctccgaagga tgcttgatat acgtccacgc
      121 gatctcgctg atcggaagca gcccatccat cccgccaaga tccacaaagg cgccaaagtc
      181 cgtgagccgc ttcaccgacc cttcaacgat ctgcccttcc tgcagcgact cgaggaggtc
      241 cgccctgcgc ttgtgccgct cctcctccac ggcctgccgg tgcgaaagga tcacgcggcc
      301 tttgggccgg tcaacctcga tgactttaag cgggaccgat tggccgacaa accactcgaa
      361 tctgcgacct ttcgcttgag tgagatcaac gtgggacccg ggcacgaatc cccgcatgcc
      421 gaggtccacc acgagtccac ctttgacctt gtctaccacc atcgcatgga ggaccgcgcc
      481 gctctcctta gcttccaaca cgcgacgcca ggcaacctcg aagtctgcgc ggcgcttgga
      541 gaggatgaga ttcccctcgt cgccctcgac acgcattacc atcacgtcga tgcgctcacc
      601 gacactgacc tcaggctcac cctgcttccc gagctcattc ggcggaatca atccctcgga
      661 cttcgcgccg acatcgacca gcacgccttc ggtatcgatg cgtacaacgg tgccctgcac
      721 aatttgtcgc ggagaaagat tcggaatttg ctcgagttgg ggttgatctt ctgaagtttt
      781 catctgctga aaacggctaa ccatacttcg cgtacggctc agccgttcct ccacatggtc
      841 atgttcgccg atttacgccg gcgctgcgcc catcaacacc gtgcggacgc gctctggcat
      901 tcgctgcaca cgcccggcca gatccgtcgc cgcgtgcacc gtgtacccgc tgaccagcaa
      961 tgtgtcatcc gattgtaacc gcacttcgta acgaaacgtc agcgtcgcct tcgagacttc
     1021 gccgaccgtc gtgcggacga caatcaggtc gtcatatcgc gctgcccgtt tgaactcggc
     1081 gtgcgcctca atcacgacga gccggacgcc atcctgctct acttctcggt acgggaggcc
     1141 catcgcccgg gtgtactctg tccgcccgac ctcgaaccac acgagatagc tcgcatggtg
     1201 gacgacaccc atcgcgtccg tctcggcgta acgcacacgg aacgtgttgt ccacgatccg
     1261 ccggccattt ccaaagggcg cgcggtctgc cggcatcttc ggtgcccttt ggcggaaccg
     1321 ccgcgaaacc cttgccatta cacacggctg gacggtctca cgagttccca gcgtccgggg
     1381 tacgtggtac cctttcggcc caaacagggt acctgctacc tttttggctg gaaagtgagg
     1441 gttttagatg ttccagtagc tgcgggcggc gctgcggatg gcgcggacgc gggtggctac
     1501 ctgctgataa gggagctcca aatgaggttc atccctctct ccgctgagcg ccgtgcggcc
     1561 catgaaggcc tcgagcgtag ccacggccga gcggccgagg tgagcaagat gataacctcc
     1621 ctcgagcgcc acggccatgg gcgtctcgtg atggaccgcc atcgtgacga ggagttctga
     1681 gagcattcgg aagccggcct gggagagtac catcacgccc aggggatcgt cgaagtgcgc
     1741 atcatatcct gcggagatga gcaccagctg cgggtcgaat gcgttgagaa ccggcaggac
     1801 gatctcttca aacaccagcc ggtagccttc gtcgcccgtg ccggccggca acggaatgtt
     1861 gagcgtgaac ccttcgccct ccccctcgcc ctgctcctca atattgccgg tgcctggata
     1921 ccagttctct tgatgggctg aaatgtacaa cacggagccg tccgtgtaga aaatctcctg
     1981 tgtgccgttc ccgtgatgca cgtcccaatc cacgatgccg atccgcacca ggccgtatcg
     2041 ctcacgcgcc tcggccgcgg caatcgcgac gttgttgaac agacaaaacc ccatgccggc
     2101 cgcggggcga gcgtgatgcc cgggcgggcg cacgacggcg aaggccgccc ggtgctgacg
     2161 attcatcact gatgacacag ccgccacgat gccgccagca gcgagtcggg cgacgtcgaa
     2221 tgagttggcc gatacgacag tgtccgggtc gagccggccg ccgccctccc cggcgagatt
     2281 cttcaccgcg tcaatgtaag aacgatggtg tacgagctcc aaaactttgc catcaaccgg
     2341 ctccgggatg atctgggtga ggtgttcttg caggccgctt gtttctatgg ctgcgctgat
     2401 ggcgcgcatg cgctccggcc gctccggatg acctggtccg gtcttgtgtt tggtgtaatc
     2461 ggggtgcgtg acgaggacga cgctgcctac tgccttctac cctcggcctt cgcagttgcg
     2521 tttgcggcag cctttaatcg ccgggcggcg gcctcaggac cgcccacccc tttgaagatg
     2581 gccgatgccg cgacgagtac gttcgctcct gcgtccaccg cgcgcggcgc cgtgctctca
     2641 ttgatgccgc cgtcgacctg cacatcgaga tccagcccac gctcctcaat ccacgaacgc
     2701 agctggccaa cttttgagag aacagatggg atgaactgtt ggcccgcata acccggattg
     2761 accgtcatca acagcaccat gtcgaggtcg gaaagcaccg gctcgacggc accgagcggc
     2821 gtgcctgggt tgagggcggc ggcggctctg actccaaggt ccttgatctg ctgaaccacg
     2881 cggtgcagat gaggcacggc ttccacgtgc actgtcatcg atgccgcgcc ggctcgggcg
     2941 aacgtcggca cgtgccgctc agggtccatg atcatcaggt gaacgtcgat tggccgccgt
     3001 gtcgcacgct tcgcggcctc gacaattact gatccaatcg tgatttcggg gacaaaccgg
     3061 ccatccatga cgtccacgtg gatcgtgtcg acgccaccgc gctcggccgc ggcgatctcc
     3121 tcgccaagcc gcgtgaaatc agcggacagg atgctggccg caatggtgac cgcgctttca
     3181 gccgtcacgg gcggacttct tggaccagct ggttctggac aaacacctgg atgatcgtga
     3241 acccgcgcga ccggatccgc tcgtcgacgc ggtccccggg ggcgagggtc ttctggaaga
     3301 cggtttgcac accgcgctca tcgatgacga cgatcttgac gtcctgttcc gggtcgccac
     3361 caggcacgac gagctgcacg cgcgtcagct tttcttctgg agagggagga cgctgcggct
     3421 gcgctgtgac gaccggtgaa ggcggcggta tggtttcctg cccgggccgc acggtcaccg
     3481 tgaccgtcac ggcgtcctgg gaacggattc gggtacctgg tgatggcgat tggtccacga
     3541 cagtctcagg gttcatgtct gcggtcggca ctgtcttcac cgcggccaac gtcacgccga
     3601 gttcttgcag catgcggcga gcctccagga gagagcgacc aaccaggagg ggcatctgca
     3661 cgcgctgtgg gcctttactg acgacgagat ggatgggaga cccacgctcc agccgcgcgc
     3721 cgggctgcgg attctgcgag atgacgaagc cgcccttgac atcttcatcg aaagcttcgc
     3781 gcagctcacc gatctggagg cgcgcgcgat cgatgatcaa cctggcctcg atcagcgcac
     3841 gccgctgcac atccggaact cccaccatct ccggaccgag gctaacggca acattgacga
     3901 cgcggccgac tttgacaggt ttcccggccg gctgatcctg cgagatgatc aggccggcgg
     3961 cgacgtcagt gctgaaggcc tcctcggtca cgcggagggt gagtcttcgc tcggatgccg
     4021 cctgctcgcc ggcctgcacc gtcttcccga cgaaattcgg cacctcgact tccggaacgg
     4081 cgatgtagct gctaaacgcg cgccatccgc cccagacacc cccgatgacc agcgcgatca
     4141 gcccgagggc gaggaccggg ccgggaacca agaggcggcg ctcatctaac tcctgcgtcg
     4201 cacccggccg caggaccatc gtcgccggcg tgtcttcctt cgttcgcttt gggggcggca
     4261 ggtctttcca gaggtcggtc ttcccgagca gatcccaccg catttgatcg gccgattggt
     4321 agcgatagcg cggcgcctta gccagcgcgc gcaggataat accctcgccg cgcggcggca
     4381 gatccgccac gtgagtcgac ggcatcgggg gatcttgatc catgtgcttg agcgcgatcg
     4441 cgatcggact gtcctcatca aacggaaggg cgcctgtgac catctcatag aggaccacgc
     4501 ccagcgcgta cacgtccgcg gtgcgcccta cgggcaagcc tcgcgcctgc tccggcgcca
     4561 gatactggac cgaacccagg accgttccgg tctgagtgat cgtcgccgag ctgagggcac
     4621 gcgcgatgcc aaagtcggcg accttgacct cgccgccctt cgtcagcagg atgttttgcg
     4681 gtttgatgtc gcgatggatg atgccgtggg catgcgcgta ctcgagggct tcgcatacct
     4741 gggcggcgat ctcagccgcc cgtgaccatg gcagccggcg ctcgcgacgg aggaggcgct
     4801 tgagatcttc gccttcgacg aactccatcg caatgtagtg gatgccgttg tcttctccgc
     4861 tggcgaagac acggaccatg tgaggatggg acagttcgct gacggccttg gcctcgcgct
     4921 gaaagcgctc cacgaactcg gcatccgcgg cgtactgctc gcgcaagacc ttgagggcca
     4981 gcacttgacc atcgctggtt cgccggaccg agtagacggt ggccatgccg ccttcactga
     5041 tgcggctcag gatttcatac tcaccggcga ttacccgtcc ttgcgtaatc acgtcaacgt
     5101 ccgtccccta tttccgccgc cagatacctt gccccttccc tctctccatg atccactagt
     5161 ctctcatttg cgcgcttacg cgtttgcgcg cttgcgcgtg tccgcgaact ccggtccatg
     5221 ggttcttctc agctgcccgc atgccgc
//
LOCUS       DBNGSB010000105         3139 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3905687, whole genome shotgun sequence.
ACCESSION   DBNGSB010000105 DBNGSB010000000
VERSION     DBNGSB010000105.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3905687"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="AC159B_02875"
     CDS             <1..1003
                     /locus_tag="AC159B_02875"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR3376726.1"
                     /translation="FTHYTPSAGLYEAREAVAEHVSVTRGIPVDPDEVVMTPGSKPIM
                     FFAILALVNPGDEVIYPDPGYPIYESVARFAGAVAKPLVLREARGFRIDPEELRALVT
                     PKTRLIVLNSPHNPCGSVLTRADIEAIAEIVGRSNAMVLADEIYWQIMYDSVHVSLVS
                     IAGMKDRTIVLDGFSKAYAMTGWRLGFGVMRKDLAGKMAQLMVNSNSCAAAFTQIAGI
                     AALRGPQEPVKKMVAEFRRRRDVIVQGLQAMEGVTCAVPPGAFYAFPNVTKVDPDSKR
                     LADFLLTEGGLACLSGTAFGEHGRGYLRFSYANSVQNIEEGLKRMRESLRRYKTAVS"
     gene            complement(1030..1485)
                     /locus_tag="AC159B_02880"
     CDS             complement(1030..1485)
                     /locus_tag="AC159B_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376727.1"
                     /translation="MERQQDSRYGFTITRPSAWVRVEEGQSAVRFVPPGHAERSTSAP
                     EFVLVQTSPSGGILNDGDIRRAVFSLLSVHGVSGFQQDARTSPQVLWYKFEVTGATEG
                     TEWASVGLVASGPRRYQVAVCAKPLDRWRDGQKQCDEIIRSFQPGNLSE"
     gene            complement(1582..2409)
                     /locus_tag="AC159B_02885"
     CDS             complement(1582..2409)
                     /locus_tag="AC159B_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376728.1"
                     /translation="MDLFLLGARRFAAACLITTMAVATAWAQGPTVDQLLVRLFTSEN
                     EEPYELTADFSGKLSLFVRGSRVTAYAEGSFREWRKAGDVRRRKVTIDRLDVPLLLRP
                     FAGTLRKIIEEKVETQTENQETFHSHDIFMQADLPGRQYALGGVHRGIVDDAIGRYGK
                     PEDKLDIATRRRIAQWLFTNPNMRSMLVRSGPPYAMRAVVDTDGHVYELTLFYDWGQI
                     GTKITYTVINGVPVWRNVTSDTVGDLSGVGRVEGDLVLVFSNHCMNCVRGPSPPPRR"
     gene            complement(2418..2981)
                     /locus_tag="AC159B_02890"
     CDS             complement(2418..2981)
                     /locus_tag="AC159B_02890"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR3376729.1"
                     /translation="MNACAMDAYRCIVSKKDLRTFTDAPIPEDVLRKILNGGRMSGSS
                     RNRQPWRFVVVRERERLASLARFGRFAQHVAAAAAAVAIVIEDAGYAFDAGRCAQNMM
                     LTGWNFGVASCPATPHQQDDAKRFLEIPQGKTLPILVSFGYPRAAGRGPIERTALRIL
                     AGRGRRPLDATVYWERYGNAIPLNHRV"
BASE COUNT          591 a          967 c          973 g          608 t
ORIGIN      
        1 cttcacccac tacacgccgt cggccggact gtatgaagca cgcgaagcgg tcgccgagca
       61 cgtctcggtg acgcgcggga tcccggtcga ccccgacgag gtcgtgatga caccggggag
      121 caagcccatc atgttctttg cgattcttgc gcttgtgaat cccggtgacg aggtcatcta
      181 tcccgatcct gggtacccga tctatgagtc ggtcgcgcgg ttcgcgggag ccgtcgccaa
      241 gccgctcgta ttgcgcgagg cgcgcgggtt ccgcatcgat cccgaggagc tccgagctct
      301 cgtgacgccc aagacgcggc tcatcgtcct gaacagcccg cataatccgt gcgggtcggt
      361 gctcacacga gccgatattg aggcgatcgc ggagatcgtc ggccgctcga atgcgatggt
      421 actggcggac gaaatctatt ggcaaatcat gtatgacagt gtgcacgtca gtctcgtcag
      481 tattgcgggg atgaaagatc gcacgatcgt tctcgacgga ttctccaaag cctatgccat
      541 gacgggatgg cggcttgggt tcggggtcat gcgtaaagat ctcgccggga aaatggcgca
      601 gctgatggtg aactcaaact cttgcgcggc cgccttcacc cagatcgccg gcattgcggc
      661 actccgcggc ccgcaggaac cggtcaagaa gatggtggcc gagtttcgac gccggcgcga
      721 cgtgatcgtc caggggctgc aagcgatgga gggagtcacc tgcgccgttc cccccggtgc
      781 attctacgcc ttcccgaatg tcaccaaagt ggatcctgat agcaagcggc tggcggactt
      841 ccttctgact gaaggcgggc tggcctgtct ctccggcacc gcattcgggg agcatggcag
      901 gggctatctg cgattctcgt acgccaacag cgtgcaaaac atcgaagagg gtttaaagcg
      961 aatgcgggaa tctctcaggc gatataagac tgcagtaagc taactacggg ataaggggat
     1021 atcccgcagt tattcactca gattgccagg ctggaaactt cgtatgattt cgtcgcactg
     1081 tttctgtccg tcccgccagc gatccagtgg tttggcgcat actgcgacct gataccgccg
     1141 cgggccgctc gcgacgagcc caacggatgc ccactcggtg ccttccgtgg cgccggtcac
     1201 ctcgaacttg taccagagaa cctggggcga ggtgcgtgca tcctgctgga accccgacac
     1261 accatggacc gagagcagcg agaagacggc ccgtctgatg tcgccgtcgt tgaggatacc
     1321 tcccgaaggc gaggtctgaa ccagaacgaa ctcgggtgct gaggtcgacc gctccgcgtg
     1381 tccgggcggc acaaagcgta ccgcgctctg gccttcctcg acgcgtaccc atgcggaggg
     1441 ccgtgtgatc gtgaacccgt atcgcgagtc ctgctgccgt tccatgcgtg tggacgcgcg
     1501 ttctaggcag cccgaaacca ggaacacgag cagaaggatg acgcaaattc gtgggatcac
     1561 ggttcttgga cgagtgaagt gttaccggcg cggaggtgga gacggtcccc tgacgcagtt
     1621 catgcagtga ttgctgaaca ccaggaccag atctccctcg acgcggccca ccccgctcaa
     1681 gtcgcccact gtgtccgatg tgacatttcg ccagacaggg acgccgttga taaccgtata
     1741 ggtgatcttc gtccctatct gaccccagtc atagaagagg gtcagctcgt acacatggcc
     1801 gtcggtgtcg accaccgccc gcatggcgta cggaggtccg gagcggacca gcatgctccg
     1861 catattgggg ttcgtgaata gccactgtgc gatgcggcgg cgggtggcga tatcgagctt
     1921 gtcttcaggc ttgccgtacc ggccgatggc gtcgtcgaca atccctcggt ggacgccgcc
     1981 cagcgcgtat tgccgcccgg gaagatcagc ctgcatgaag atgtcatgag agtgaaaggt
     2041 ctcctggttc tccgtctgcg tctcgacctt ctcttcgatg atctttcgca gcgttccggc
     2101 gaatggccgt aacaggaggg gaacatcgag tcgatcaatc gtcacctttc gacgccggac
     2161 gtccccggct ttgcgccact cgcgaaatga tccctccgcg taggccgtga cgcggctgcc
     2221 gcggacgaaa aggctgagtt ttccggagaa gtccgccgtg agctcatacg gctcttcatt
     2281 ctcagaggtg aaaagccgga cgagcagctg gtccacggtc ggaccttgcg cccatgccgt
     2341 ggccaccgcc atcgttgtaa tcaggcacgc ggcggcgaag cgacgagcgc ccaggaggaa
     2401 taagtccacg acgcccatta tacccggtga ttcagtggga tggcattccc atatcgctcc
     2461 cagtacactg ttgcgtccag gggccggcgt ccgcgacctg cgagaatcct gagtgcggtg
     2521 cgctcgatcg gtccccgtcc ggctgcgcgc ggatacccga atgacacaag aatcggcagt
     2581 gtctttccct gagggatctc caggaatcgt ttcgcatcat cctgctggtg cggcgtggcc
     2641 ggacacgacg cgaccccgaa gttccagccg gtgagcatca tgttctgcgc gcagcggccg
     2701 gcgtcgaacg cgtagcccgc gtcctcaatc acgatggcca cagcggccgc ggcggcggca
     2761 acatgctgcg cgaatcgccc gaaccgggcg agcgaggcca accgctcccg ttcacggacg
     2821 acaacgaacc gccacggctg gcggttccgg ctgctgccgc tcatccgccc gccgttgagg
     2881 atcttgcgca gcacgtcttc gggaattggt gcgtctgtga acgtgcgcag gtctttcttg
     2941 ctgacgatgc accggtaagc gtccatggcg cacgcattca tcgccgtcgc gcatttctcc
     3001 ctcaaatgtg ccgcaatcgc gcaaacgcgt aaacgcgcaa acgggcaaac gcgcaatcgg
     3061 cggggatcga ctgatttgcg cgcttccgtg cttgcgcgct tccgtttgtt agaatctgtg
     3121 catgctgcgc cgacttccg
//
LOCUS       DBNGSB010000106         5034 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_71026, whole genome shotgun sequence.
ACCESSION   DBNGSB010000106 DBNGSB010000000
VERSION     DBNGSB010000106.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 5034)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5034)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5034
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_71026"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..760
                     /locus_tag="AC159B_02895"
     CDS             137..760
                     /locus_tag="AC159B_02895"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HIR3376730.1"
                     /translation="MPYPFKLPELGFPYNALEPHIDAQTMEIHYTKHHQAYVNNLNAA
                     LEKHAAFQKKEVEDLLSSLSDLPTEVQNAIRNNGGGHLNHTMFWQWLKPGGSKEPTGK
                     LADEIRTTFGALDTFKEQFAQAATSRFGSGWAWLAADRSGKLKIYSTANQDAPVLEGN
                     RPILGLDVWEHAYYLKYQNRRPDYVKAWWNVVNWSAAAQAFEKIKGK"
     gene            885..1793
                     /gene="gnd"
                     /locus_tag="AC159B_02900"
     CDS             885..1793
                     /gene="gnd"
                     /locus_tag="AC159B_02900"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HIR3376731.1"
                     /translation="MELGMIGLGRMGANMSERLVRGGHRVVGYARTQESVQRVVKFGA
                     EGARSLADLVENLRPPRVIWLMIPAGDPVDQTLATLQPLVRAGDTIVDGGNSYYKHTV
                     RRAAMLSDHNINYVDVGTSGGIWGLTEGYSLMIGGPSEAVTRLRPVFETLAPGSDKGW
                     GHVGPHGAGHFVKMVHNGIEYGMMQALAEGFTILKRKKELDLKIDQIAEIWRHGSVVR
                     SWLLDLTARALKDDQELSDVVPYVADSGEGRWTVFEAVDLDTAAPVITLALMQRLRSR
                     DTTAYAERLLALMREQFGGHEVKRGS"
     gene            1799..2542
                     /gene="pgl"
                     /locus_tag="AC159B_02905"
     CDS             1799..2542
                     /gene="pgl"
                     /locus_tag="AC159B_02905"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HIR3376732.1"
                     /translation="MRGVNVSPDIEALSRAAAEDAVLIIRSAVAQRGTCALALSGGNT
                     PRSLYRLLGTANRNQIPWEQVHLFWGDERYVPPDDPASNYRMVRETLLAAIPIPAGHV
                     HPMPTNQEDPNDAAREYEVLLKRQFNDDWPRFDLILLGLAPDGHIASLFPGSKALVEE
                     KQRVLAVRVPKEPPQRLTLTLPAINHAANVHFLVAGRSKAGALHRVLVGPCDVQTCPA
                     VGVKPINGTVTWWVDEAAAAFLGARLSES"
     gene            complement(2528..3109)
                     /locus_tag="AC159B_02910"
     CDS             complement(2528..3109)
                     /locus_tag="AC159B_02910"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR3376733.1"
                     /translation="MNLRRDISKSLLWTVAAIAVVITVIFIAARFTTQNVREHAQPAP
                     DFEVRMYQGQADVGGQQVRLSQILARGKPVVLNFFAGLCPPCRAEMPDFQERYAAGGR
                     DRYVMLAVDVGPYTALGTREDGKRLLGTLGITFPAGTVFDEDILAAYQLFGMPSTFFI
                     TPRGRIVRKQAGLLTPGQMDAYIRELLEASALR"
     gene            3112..3351
                     /locus_tag="AC159B_02915"
     CDS             3112..3351
                     /locus_tag="AC159B_02915"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_463674418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:3148..3150,aa:Sec)
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HIR3376734.1"
                     /translation="MAKTVVVYSQPGULFCGKVKEFLHQKGIAYTERDISADEKAFDE
                     LVNKGFSATPVTIIDGEAVVGFNRGTLEQLLFSED"
     gene            3352..3759
                     /locus_tag="AC159B_02920"
     CDS             3352..3759
                     /locus_tag="AC159B_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006366705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIR3376735.1"
                     /translation="MGLSEDQVQQILTTTKTIAVVGLSNKPDRPSYDVASYLKEHGYR
                     IIPVNPTIKEALGEKAYASLKDVPGRIDVVQIFRRPEDVPAVVDDAISIGAKVVWMQP
                     GAENEEAAAKAEAAGLKAVVGACMRTVHRTLRH"
     gene            3767..4519
                     /locus_tag="AC159B_02925"
     CDS             3767..4519
                     /locus_tag="AC159B_02925"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: protein motif:HMM:NF014198.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HIR3376736.1"
                     /translation="MKESVRAKAVTGLLVALRERSPRVMCLGAGRSGSISGAIQALGA
                     KVVPGIAGHFTRNLAGVDVLLLSPMKTATGRRQMDEALAAARHLGMPVVIDLTGALKL
                     RRSVPAVRRMIAAATWPVLSSTSDELAAFANPAGKRTDPVRLAAEILQSGGLAAIRGS
                     TGMVTNGKNVLRVDAGHDWLETAAPAGGLAAGSIAAFLSVAHLADFITVTAVALACLG
                     QAADRASRRAKSQPMEKRVVKELGALSAGAAR"
     gene            4519..4998
                     /locus_tag="AC159B_02930"
     CDS             4519..4998
                     /locus_tag="AC159B_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HIR3376737.1"
                     /translation="MRTSATDRVRAALETLGVHVQILEFSHSTRTAQDAAVAVGTTVA
                     QIVKSLVFLADSRPVLVLASGANRVDPVRLASAATAAKARMASAEEVRDITGYSIGGV
                     PPVGHATPLSIYLDQDLMRFEILYAAAGTPSAVFGISPQLLRDITGARVVAIAAENL"
BASE COUNT         1025 a         1490 c         1607 g          912 t
ORIGIN      
        1 agacatacga gactagtctg tttcgcacgg cgcaggacca cacctccgct agttctgcct
       61 tgacagtcca agggagcggg gtaaaactga gggagtccct gacatcctgc tttgattcct
      121 gagagggggt cgtagaatgc cgtatccgtt caagctaccc gagctggggt ttccgtataa
      181 cgctcttgag ccgcacatcg acgcacagac gatggagatc cactatacca agcatcatca
      241 ggcctacgtg aacaacctga atgcagccct ggagaagcac gcagcgtttc agaaaaagga
      301 agtggaagac ttgctcagca gcctttccga tctgcccaca gaggtccaga acgccattcg
      361 gaacaatggg ggcgggcacc tcaatcacac catgttctgg cagtggttga agcccggcgg
      421 cagcaaggag ccaactggga aacttgccga tgaaatccgg acgacgttcg gtgcactcga
      481 tacgttcaag gagcagttcg ctcaagccgc gacgagccgc ttcgggtctg ggtgggcctg
      541 gctggcagca gaccgcagcg gcaagctcaa gatctactcg accgccaacc aggatgctcc
      601 tgtcctggag ggcaacagac cgatccttgg gctcgacgtt tgggagcacg catactacct
      661 gaaatatcaa aaccggcgtc ctgactatgt caaagcatgg tggaacgtcg tgaactggag
      721 cgccgcggca caggcgttcg agaagataaa ggggaagtag ccgaatttgc tcgtcatgta
      781 gatccggcgg ccggatatcg tccggccgcc tggtatcttg tgcactgcgg tcgtaggtcg
      841 taggtggtta cgacctacga ccctgtagtt tgcgaggaga tgcgatggaa ctcggcatga
      901 tcggactcgg gcgcatgggc gccaatatga gcgagcgcct ggtgcgagga gggcaccgcg
      961 tggtgggtta cgcgcgaacc caagagagcg tgcagcgcgt cgtaaagttc ggcgcagaag
     1021 gcgcacgctc gctggcggac ctggtggaga acctgagacc gccgcgcgtc atctggctga
     1081 tgatccccgc gggcgatccg gtggaccaga cgctggcgac gcttcagccg ctggtccgcg
     1141 cgggtgacac catcgtcgac gggggcaact cgtactacaa acacaccgtg cggcgggcgg
     1201 cgatgcttag cgaccacaac atcaactacg ttgatgtagg gaccagtggc ggtatctggg
     1261 ggttgaccga aggctacagt ctgatgatcg gtggtccgag cgaggcggtc actcgattgc
     1321 ggcccgtctt cgagaccctt gcgcccggat cggacaaagg ttggggacac gtcggcccgc
     1381 acggcgccgg acacttcgtc aagatggtgc acaacggcat cgagtacggc atgatgcagg
     1441 cgctggccga agggttcacg atcctaaagc gcaagaaaga actcgatttg aagatcgatc
     1501 agatagctga gatctggcgg catggaagcg tggtgcgctc gtggctcctg gatctgacag
     1561 cgagagcgct aaaggatgat caggaactgt ccgacgtcgt gccctatgtc gctgactctg
     1621 gagaggggcg gtggacagtc tttgaagccg tggatctgga tacggcggcg cccgtgatca
     1681 cgcttgccct catgcagaga ctgcgctcgc gcgacacgac cgcgtatgca gaacggcttc
     1741 tggcattgat gcgagagcaa ttcggcggtc acgaggtcaa gcgaggaagc tgacactagt
     1801 gcggggcgtg aacgtatcgc cggatatcga ggcgttgagc cgggctgcag cggaagacgc
     1861 ggttctgatc atcaggtctg ctgtggcgca gcgcggcacg tgtgcgctgg ccttgtccgg
     1921 aggcaacacg ccgaggtcgc tctaccggtt gctcggcacc gcaaaccgca accagattcc
     1981 ctgggaacag gtgcacctgt tctggggcga cgaacgatac gtccccccgg acgatccggc
     2041 gagcaactat cgcatggtgc gggagacact tctcgctgcg attcccatcc ctgccgggca
     2101 cgtacatccg atgccgacga accaggaaga ccccaacgat gcggcccgcg agtacgaggt
     2161 tctcctgaag cggcagttca acgatgattg gccacgcttc gatttgatcc ttctcggact
     2221 ggctcccgat ggacacatcg cttcgttgtt ccctggatcg aaggcgctgg tagaagagaa
     2281 gcagcgtgtc ttggctgtcc gggtgccgaa ggagcctccc cagcgactca cgctgacgct
     2341 gccggcgatc aatcatgcgg ccaacgtcca ctttctcgtg gccggacgtt cgaaagccgg
     2401 cgcgctgcat cgggtgcttg tcggaccctg tgacgtgcag acctgccccg cggtgggcgt
     2461 gaaacccatc aacggaaccg tgacctggtg ggtcgacgaa gccgccgcgg cattcctcgg
     2521 cgcacggcta tctgagagct gacgcctcca acagttctct gatgtatgcg tccatttgac
     2581 ccggcgtcag caagccggct tgtttgcgga cgatccggcc tcgaggcgtg ataaagaacg
     2641 tcgacggcat tccaaagagt tgatacgcgg ccagaatatc ctcatcgaag accgttcccg
     2701 ccggaaacgt gatgccaagc gtccccagca gcctttttcc gtcctcgcgc gtgccgagcg
     2761 cggtatacgg accgacgtca acggccagca tcacgtaccg atcccgcccg cccgcggcgt
     2821 aacgctcctg gaaatccggc atctcggcac gacacggcgg gcacaagccg gcgaagaagt
     2881 tcaggacaac cggttttccg cgggcgagaa tctgcgagag ccggacctgc tgcccgccca
     2941 cgtcggcctg tccctgatac attctcacct cgaaatcggg agccggctga gcatgctcgc
     3001 ggacgttttg cgttgtaaat ctcgcagcga tgaatatgac tgtgatgacg accgcgatgg
     3061 ccgccacggt ccagagcagg cttttggaaa tatctcggcg caagttcata tatggccaaa
     3121 accgtcgtcg tgtactcgca gcccggctga ctgttctgcg gcaaggtgaa ggagttcctt
     3181 caccagaaag ggatcgccta cacagaacga gacatcagcg cagacgagaa ggcgtttgat
     3241 gagctcgtca acaagggctt ctccgcaacc ccggtgacta tcattgatgg agaagcggtt
     3301 gttgggttca accgagggac gctcgagcaa ttgttatttt cggaggacta gatggggctg
     3361 tcagaggatc aagtgcagca gatcctgacg acgacgaaga cgatcgccgt cgtcgggctg
     3421 tcgaacaaac cggatcgtcc cagctatgat gtcgccagct acctcaagga gcacggctac
     3481 cgaatcatcc ccgtgaatcc aacgatcaaa gaggcactcg gagagaaagc ctacgccagc
     3541 ctgaaggacg tccctggccg tattgacgtc gttcagatct tccggcgccc cgaagacgtg
     3601 ccggctgtcg tcgacgacgc catctccatc ggagccaagg tcgtgtggat gcaacccggc
     3661 gcagagaacg aagaggccgc ggcgaaggct gaggctgcag gcctcaaagc cgttgtcggc
     3721 gcctgtatgc ggacggtgca tcgaacactc cgtcactaac tcccagatga aagagtcggt
     3781 ccgcgcgaag gctgtgacgg gcctgctggt tgcgctgcga gaacgcagtc ccagggtcat
     3841 gtgcctgggc gccggtcgat caggttcaat atcaggggcc atccaggcgc tcggggcgaa
     3901 ggtcgtcccg gggatcgcag gccatttcac ccgcaatctc gcaggcgtgg acgtgctact
     3961 cctatcgccg atgaaaacgg ctaccggtcg gcgccaaatg gatgaagcgc ttgccgccgc
     4021 ccgacaccta ggtatgcccg tcgtcatcga tctcacgggt gccttgaaac tccgacgctc
     4081 agtgccggcg gtgcggcgga tgatcgcggc cgcaacctgg cctgtcctgt ccagcacaag
     4141 tgacgaactg gccgctttcg cgaacccggc tggaaagcgc accgatcctg tccggctcgc
     4201 ggcagagata ctccaatcgg gcgggctcgc ggccatacgc gggtcgacgg ggatggttac
     4261 caacggcaaa aatgtgcttc gggtcgacgc cggccatgac tggctggaaa ctgcagcgcc
     4321 ggcaggaggg ctcgccgcag ggagcattgc agcgtttctc agcgtcgccc acctcgccga
     4381 cttcatcacg gtgacggcag tggcgctcgc ctgtttgggg caggccgccg atcgagcttc
     4441 gcgccgcgcg aaatcacaac cgatggagaa gcgcgtcgtc aaggagctgg gcgcgctctc
     4501 cgctggagct gcgcggtgat gcggacgtct gcgaccgatc gcgtccgagc tgcgctggaa
     4561 acgctgggtg tgcacgtcca gattttggaa ttttctcaca gtactcggac agcgcaggac
     4621 gccgccgtgg ctgtgggaac cacggtggca cagatcgtca aatcactggt attcctggcg
     4681 gactcgcgtc cggttctggt gcttgcgtcg ggcgcgaacc gcgtagatcc cgtgagactc
     4741 gcatccgcag cgaccgccgc gaaggcgcgg atggcgagcg ccgaggaggt ccgggatatc
     4801 acggggtact ccatcggcgg cgtgccgccg gtcggccatg ccacgccgct cagcatatat
     4861 cttgaccagg atctcatgcg gttcgagatt ctctacgcgg cagcaggcac accatccgca
     4921 gtcttcggca tttcaccgca gctgcttcga gacattacgg gggcgcgcgt cgtcgcaatc
     4981 gcggctgaga atctctagaa ttccaccggc atggccttgt gtccgcggat gacg
//
LOCUS       DBNGSB010000107         5408 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_1759560, whole genome shotgun sequence.
ACCESSION   DBNGSB010000107 DBNGSB010000000
VERSION     DBNGSB010000107.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 5408)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5408)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5408
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_1759560"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="AC159B_02935"
     CDS             <1..219
                     /locus_tag="AC159B_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3376738.1"
                     /translation="RVFTRDFLLEHIWGYDYYGSTRTVDMHISRLREKIEDNPASPTF
                     IVTVRGVGYKFKPQETGTRERGNAGARN"
     gene            222..1985
                     /gene="pnpS"
                     /locus_tag="AC159B_02940"
     CDS             222..1985
                     /gene="pnpS"
                     /locus_tag="AC159B_02940"
                     /inference="COORDINATES: protein motif:HMM:NF046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system histidine kinase PnpS"
                     /protein_id="HIR3376739.1"
                     /translation="MRRSLRWKFTSSYLFLVIVVLLVVTAYVARVLERSYMNTYAYVV
                     ATQAKVISLMMRDYAVGRDLVSLQPMVQELRWRKEATITLADSTGRAADPALDPSYPE
                     VQKAFAGVESQAVRADPRTGERRVYAAAPIFGPGKVIIGIVHVSAPEAWVWRQLRRMV
                     PQFGVAMLLGLVAAFLIGTRLSRRITRPLEEFSRMADRISSGDFAGRAPVDDTEELGR
                     LSRSFNTMAGRLERVIAEISAERNKIEAIIAGMSDAVVATNREGQIILLNRAAEDLLQ
                     LERRRAQGQPARDVVPAERLVAMLEDATDRGRLTAEELPPGSMSDRVVEAHCAPITGD
                     GGNVIGAVAVLRDVTELRSTERMRRELTANVSHELRTPLTSIVGFVETLLDGAMTDEE
                     TARRFLTIIDDEAKRLVKLVDDLMDLSRLESKRVALELKPVDVGRLAYHVVDKMRPLA
                     ENFQLVLSQSVPSGLVAPADSDRLEQVLTNLVDNALKYTPPGGRVEVRASTDNGEIVV
                     EVADNGHGIPPEDLPHVFERFYRADRSRTRGSGGTGLGLAIAKHIVEAHGGRISVHSR
                     IGEGTTFLFTLPRNGSLEQPN"
     gene            2503..2994
                     /locus_tag="AC159B_02945"
     CDS             2503..2994
                     /locus_tag="AC159B_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR3376740.1"
                     /translation="MRANGNDQRLVPTRSTGTIPVVNSAHWLPNSSGLVYTSRAGVAA
                     DEIAFVQPDGSGYRWFSTGYAPTFPQDMTQLAYVVSRVGSAQVYIKPFAGGSSRPLTP
                     RTWISVRPAWSQDGQWIAYLSVKPGEQLGVWVMRPDGSRQRRLAPAAGNLEVLPLISW
                     RPR"
     gene            complement(3005..3715)
                     /locus_tag="AC159B_02950"
     CDS             complement(3005..3715)
                     /locus_tag="AC159B_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006304941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3376741.1"
                     /translation="MALLDVKDIHVYYGQIHALKGISIQVQPSEIVTLIGANGAGKST
                     TLNTISGLLRPLHGTIRFEDEDLTAIPPHNVVAKGLVQVPEGRRIFGQLTVMENLEMG
                     GFTRTDTQGMQNDLDRVFTLFPRLKERMRQVAGTLSGGEQQMLAIGRALMGGPRVLLM
                     DEPSMGLAPVLVEQIFETITDINKLGTTILLVEQNAFMALQVAHRGYVLQTGEIALSG
                     TAAELRENEDVKRAYLGG"
     gene            complement(3721..4497)
                     /locus_tag="AC159B_02955"
     CDS             complement(3721..4497)
                     /locus_tag="AC159B_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3376742.1"
                     /translation="MPILTSRSLTKRFGGLVALNQLDLDVAEQSIHSVIGPNGAGKTT
                     FFNCVTGFYKPEEGEIRFSEMSLVGLRPDEIAHSGVSRTYQNIRLFANVTAIENILVG
                     MHSHLKSAWIGAILNTPSTRREEHAAAEEARRLLHFVGMRGRGDLLARNLPYGEQRRL
                     EIGRALATRPKLLLLDEPTAGMNPKETMEMMQFIRRLRDELGITILLIEHQMRVVMGI
                     SEKVTVLDYGTKISEGTPAEVQNDPRVIEAYLGTRKVSIH"
     gene            complement(4498..>5408)
                     /locus_tag="AC159B_02960"
     CDS             complement(4498..>5408)
                     /locus_tag="AC159B_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015917728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR3376743.1"
                     /translation="YTLMGLGLNLEVGLAGLLDLGFVAFFAIGAYTVGLLISEGPLGI
                     AHWSFWAAVPVAVLFTLIAGVFFGLPILGIRGDYLAIATLGFGEITRLLVASDFLRSW
                     LGGSQGMLEIPKPVVAGFELSGPEHLFYLTLASSALVVYVAWRLQHSRLGRAWMAIRE
                     DEDVAQALGVNLVSVKLLAYGLGAAFAGVAGAIFAVMVGSVFPHSFQLLISINVLALI
                     IVGGIGSNPGVVVGALALVGLPELLREVGEFRFLVYGAVLVAMMLLRPEGLWPAAAIK
                     RELHEEEPEAVTREAIELAEAKEPNP"
BASE COUNT         1036 a         1731 c         1658 g          983 t
ORIGIN      
        1 cgggtcttca cccgggactt cctgctcgag cacatctggg gctacgacta ttacggcagc
       61 acgcgcaccg tagatatgca catcagccgc ctgcgtgaaa agatcgagga taaccctgcc
      121 tcacccacgt tcatcgttac cgtgcgggga gtgggatata agttcaaacc acaagagacg
      181 ggaacgcggg aacgcgggaa cgcgggagcg cgaaactgac gatgcggcga tcactccgct
      241 ggaagtttac gtcgtcgtat ctcttcctcg tgatcgtagt gctgctcgtc gtgacggcat
      301 acgtcgcgcg ggtgctcgag cgatcgtaca tgaacacgta cgcgtacgtg gtggcgaccc
      361 aggcaaaagt catctccttg atgatgcgcg actacgcggt tggccgcgat ctggtcagcc
      421 tgcagccaat ggtccaggaa ctgcggtggc gaaaggaggc cactatcacg ctggcggact
      481 ccaccggccg ggccgccgat cccgcgctcg acccgtcgta tcccgaggtg cagaaggcat
      541 ttgccggcgt ggaaagtcag gccgtgcggg cggatccgcg aaccggagag cgtcgcgtct
      601 acgcggccgc cccgatcttc ggccccggga aggtcatcat cgggattgtc catgtctcgg
      661 cgcccgaggc gtgggtgtgg aggcaacttc gtcgcatggt gccccagttc ggagtggcga
      721 tgttgctcgg tctggtcgcg gcgtttctga tcggcacccg tctgtcacgg cggatcacca
      781 ggccactcga ggagttcagc cggatggccg accgcatctc atcgggtgat ttcgccgggc
      841 gcgcgcccgt cgatgacacc gaggagcttg gacgattgag ccggtccttc aacaccatgg
      901 ccggccgcct ggagcgcgtg atagcggaga tctcagcgga gcgcaacaag atcgaggcga
      961 tcatcgccgg catgagcgac gccgtcgtgg ccaccaaccg cgagggccag atcatcctgc
     1021 tcaatcgtgc cgccgaagac ttgcttcagt tggaacgcag gcgcgcacaa gggcaaccgg
     1081 ccagagatgt cgtgccggca gagcgcctgg tagccatgct ggaggacgca accgatcggg
     1141 gacggctcac ggcggaagaa ctgcccccgg gctccatgag cgatcgggtc gtggaggcgc
     1201 actgcgcccc catcaccgga gatggaggca acgtcattgg agcggtggcc gttttgcgcg
     1261 acgtgacgga gctgcgcagc acagagcgca tgcgccggga gctcaccgcg aacgtctccc
     1321 atgagctgcg cacgccgctc acgtcgatcg tcggcttcgt ggagacgctt ctggatggag
     1381 ccatgacgga tgaggaaacc gcgcgtcggt ttctcacgat catcgacgac gaggccaagc
     1441 gtctggtcaa gctcgtagac gacctcatgg atctctcccg gttggaaagc aaacgcgtgg
     1501 cgctcgagct gaaacctgtc gacgtcggcc gcctagcgta tcatgtcgtg gacaagatgc
     1561 gaccgctcgc ggagaacttc cagctggtgc tctcacagtc ggtgccttcg gggttggtcg
     1621 cccccgccga ctccgaccgg ctggagcaag tcctcacgaa cctggtggac aacgcgctca
     1681 aatacacgcc gcccggcgga cgcgttgagg tccgcgcctc aaccgacaac ggggagatcg
     1741 tcgtggaggt ggccgacaac gggcacggga tcccaccaga agatcttccg cacgtgttcg
     1801 agcgattcta ccgagccgac cgttcccgta cgcggggctc gggcggcacc gggctaggcc
     1861 tggcaatcgc caagcacatc gtcgaagcgc acggcggacg tatcagtgtg cacagccgca
     1921 tcggggaagg caccactttc ctctttacgc ttccgaggaa cggatctctc gagcaaccaa
     1981 actaggttgc gtgcgctcgc actgctggtc ttcgttgcga ctgcggctgg cgcagcaccg
     2041 gctgttcaat cctctgcgct ggccttcgtc ggacttgacg aggacacccg tcagatcttc
     2101 ttgatcagaa ccgatggcgg tgggctgcgc caactcacga gtccccctgg gcacaacgaa
     2161 ggtccggtct ggtcgccgga tggtagtacc ctgagcttcg tccgaaccga cagtcgcgga
     2221 acgcagatct acatattgcg cgccgatgga tctcggaagc gtcgtctcac ggcgcctccg
     2281 ggttcacacc gctcgcccac gtggtcaccc gatggccgtt ggatcgctta tgtggaggat
     2341 cgcggaggca gcgcgcagat tttcgtcatg cgagcggatg gcagtggcaa gcgccgactg
     2401 acttcagcgc cacgtagcag tcaggcacca gcgtggtccc cggatggtac ccggatcgcc
     2461 tatctctctc ggcttcccgg ccgccaggca gaactgtttg tgatgcgggc aaatggcaac
     2521 gatcagcgtc tggtgccgac ccggtccacg ggaacgattc ccgtcgtcaa cagcgctcat
     2581 tggctgccta atagctcagg attggtatac acgtcgcggg cgggcgtggc tgcggatgag
     2641 atcgctttcg tgcaaccaga cggatctggc taccggtggt tcagcacggg atacgcaccg
     2701 acgtttccac aggacatgac acagctcgca tacgtcgtgt cgagagttgg tagcgcgcag
     2761 gtgtacatca agcctttcgc cggcgggtcc agtcggccac tcacgccgcg gacatggatc
     2821 agcgtgcggc cagcctggtc acaggatggt caatggatcg catacctgtc cgtgaaaccg
     2881 ggagaacaac ttggagtgtg ggtgatgcga ccggacggga gcaggcagcg gcgtctcgcg
     2941 ccggcggctg gaaacctgga agtgctgccg ttgatttcct ggcgtcctcg atgagtggtg
     3001 gagctcagcc tcccaggtag gcccgcttca cgtcctcgtt ttctctcagc tcggccgcag
     3061 ttcctgagag cgcgatctcg cccgtctgca gcacgtatcc acggtgagcc acctgcaggg
     3121 ccatgaacgc gttttgctcc accagcagaa tcgtcgtacc cagcttgttg atgtcggtaa
     3181 tcgtttcgaa gatttgctcg acgagcaccg gcgccagtcc catcgacggc tcgtccatca
     3241 gcagcacgcg aggtccgccc atgagcgccc gtccgatggc cagcatctgc tgctcgccgc
     3301 ccgacaatgt tcccgcgacc tgacgcatgc gctccttgag ccgcgggaac agtgtgaaaa
     3361 cgcggtcaag gtcgttctgc atgccctgcg tgtccgtgcg agtgaacccc cccatctcaa
     3421 gattttccat tacggtaagc tgcccgaaga ttcttcgccc ttccggcacc tgcaccaacc
     3481 ccttcgccac aacattgtgg ggcgggatgg cggtgagatc ctcatcctca aaacggatcg
     3541 tgccgtggag gggccggagc agtccactga tggtgttgag ggtcgtgctc ttcccggcgc
     3601 cattggcgcc gatgagcgta acaatctcgc tgggctgcac ctggatcgaa atgcctttga
     3661 gcgcgtggat ttggccgtag tagacatgaa tgtccttgac gtccaggagt gccatacgcg
     3721 ctagtggatg gagacctttc gcgtgccgag gtacgcttcg atcacgcgcg gatcgttctg
     3781 gacctcagcg ggcgtgcctt cggagatctt cgtgccgtaa tccagcacgg tcaccttctc
     3841 cgagatgccc atcacgacgc gcatctggtg ctcgatgagc agaatcgtga tgccgagctc
     3901 atcgcgcagc cgccggatga actgcatcat ttccatcgtt tctttcgggt tcatcccagc
     3961 cgtgggctcg tcaagtagca gcaatttagg tcgggtggcc agcgcgcgtc cgatctccag
     4021 ccggcgctgc tcaccgtagg gcagattcct cgccagaagg tctcctcgcc cccgcatgcc
     4081 cacaaaatgc aagagccggc gcgcctcctc tgccgccgcg tgttcttctc gccgtgtcga
     4141 cggcgtgttc aagatggccc cgatccatgc cgacttcaaa tggctatgca tccccacgag
     4201 gatgttctca atcgccgtca cgttcgcgaa cagacggatg ttctggtagg tgcgcgaaac
     4261 gcccgagtgg gcaatctcat ccggccgtag cccgaccagc gacatctccg aaaaccggat
     4321 ctctccttct tcgggcttgt agaatcccgt cacgcagttg aagaacgtgg tttttcccgc
     4381 gccgttcggg ccgatgacgc tatggatgga ctgttccgcc acatcaaggt cgagctggtt
     4441 cagtgccacc agcccaccga agcgcttcgt aagactccgt gaagtgagga tgggcatcta
     4501 gggattcggt tccttggctt ccgcgagttc aatcgcctcg cgtgtcacgg cttccggctc
     4561 ctcttcgtgc agttcccgct tgatcgcagc cgccggccac agtccctctg ggcgcagcag
     4621 catcatcgcc accagcacgg cgccgtacac gaggaaccgg aattcgccca cctctcgtag
     4681 caattccggc aaccccacaa gcgccagcgc gccgacgacc acgccggggt tgctgccgat
     4741 gccccccacg atgatcaagg ccagaacgtt gatcgaaatt agcagctgga aactatgggg
     4801 gaacacggag ccgaccatca cggcaaaaat tgccccggcg accccggcaa acgccgcgcc
     4861 gagaccatag gccaagagct tgaccgacac caggttgacg ccgagggctt gggccacatc
     4921 ctcgtcctct cgaatcgcca tccaggcgcg gccgagtcgc gagtgctgca gccgccacgc
     4981 gacgtatacc acaagcgccg agctcgccag cgtaaggtag aagaggtgct ccggccctga
     5041 gagttcaaac ccggcgacca ccggtttggg aatctccagc atgccttgcg agccgccgag
     5101 ccaggaccgc aggaaatccg aggccaccag caaacgtgtg atctccccaa accccagcgt
     5161 cgcgatggcc aggtagtcac cacgaatccc caggattgga agtccaaaga agacccctgc
     5221 gatcagggta aacagcaccg ccaccggcac cgctgcccaa aacgaccagt gcgcgatccc
     5281 gagcggaccc tcggagatga gtaatcccac ggtatacgcg ccgattgcaa agaacgcgac
     5341 gaaccccaga tccagaagac ccgcgagacc cacctccagg ttcaacccca ggcccatcag
     5401 cgtgtaca
//
LOCUS       DBNGSB010000108         3332 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_839406, whole genome shotgun sequence.
ACCESSION   DBNGSB010000108 DBNGSB010000000
VERSION     DBNGSB010000108.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_839406"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="AC159B_02965"
     CDS             complement(<1..901)
                     /locus_tag="AC159B_02965"
                     /inference="COORDINATES: protein motif:HMM:NF036997.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N' family DNA-binding
                     protein"
                     /protein_id="HIR3376744.1"
                     /translation="MTVFAEVAINAPLRAGDKTFTFSVPVALERRVTIGIPVRVPFGK
                     QTTSGYVVGLSPTAGREVKPVAALDERLPVLPSDLVTLAAWMADHYVCSVGEAIDTML
                     PPKAQSARRSSLPTSRPNPASQREQEKEVDASGNGAGVVDLLTAESAARIVIIGEDAR
                     FEAYTGALLWALQHGRGAIALEPEFAQAERLAAWIERKIGVSVALYHGAQSDPERRKL
                     WQRIASGEFKVVAGTRVAVFAPLADVGLIIVDHEEDTSYKEERVPRYHVRAIAETRAR
                     IAQAALVWGSPTPSLEVVREVEEG"
     gene            complement(1036..1596)
                     /locus_tag="AC159B_02970"
     CDS             complement(1036..1596)
                     /locus_tag="AC159B_02970"
                     /inference="COORDINATES: protein motif:HMM:NF014494.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HIR3376745.1"
                     /translation="MAQNRLQGKLIVVGVTGGIAAYKAAYLISALRKQGADVHVVMTA
                     SAAQFVQPLTFRTLSQNAVITDMWDPNNPYEEPHVYLGERADLLVIAPVTAQTIAKFA
                     LGLADDVVTATALATRAPVLLAPAMSDIMYEHPSTQEHLKTLRGRGCRVIGPEVGHLA
                     SGKEAIGRMSEPDAILEEIAAILEAR"
     gene            complement(1610..2236)
                     /gene="cmk"
                     /locus_tag="AC159B_02975"
     CDS             complement(1610..2236)
                     /gene="cmk"
                     /locus_tag="AC159B_02975"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0036431 - dCMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HIR3376746.1"
                     /translation="MIVAIAGPIGVGKSTVAKLLAQQLGYRYISGGEVFHDIARQRGI
                     SLTDVNKLAERDSSLDHEVDRRQRELAQRGDCVVESRLSGWMVEADLKVWLKAPSQIR
                     AERVAAREGRPVAAAQAELVERERSEWARYEQLYRINVDDLSPYHLVIDTTKWSAEAI
                     AQALVGFVGATPKIARPGARKQSPRAGSRGRTRLRGSTGRGPRSGKRR"
     gene            complement(2233..2511)
                     /gene="remA"
                     /locus_tag="AC159B_02980"
     CDS             complement(2233..2511)
                     /gene="remA"
                     /locus_tag="AC159B_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011431477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular matrix/biofilm regulator RemA"
                     /protein_id="HIR3376747.1"
                     /translation="MNSLADPRLINVGFGNIVAANRIIAIVAPDSAPIKRIIQEARDR
                     GTLIDATYGRRTRAVVITDSGHVVLSAVQPETVAHRFTDREGNQENPE"
     gene            complement(2504..>3332)
                     /locus_tag="AC159B_02985"
     CDS             complement(2504..>3332)
                     /locus_tag="AC159B_02985"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HIR3376748.1"
                     /translation="RFLVEIRSINHRFGEVLVRLPRDLSPLEERVRGTVQSRVSRGRV
                     EVSIMRDNRATRVRAIRPDTELAAAYTQALTDLARVLGIGGEVTLPQIASFPDVIKVE
                     EPPEDVEGIWPLLSQGLDEALSELVVMREAEGARLTADLRTRLERIEARAKEIESRAT
                     TVVADYSARLRQRITELLGHIPVDEQRLVTEVAVFAERSDISEEITRLRSHVVQFRED
                     LSNHAGAIGRKLEFVLQEMGREANTIGSKANDLEIGRAIIAIKGELESLREQVQNLE"
BASE COUNT          569 a         1091 c         1013 g          659 t
ORIGIN      
        1 gaccctcctc tacttctcga acgacctcca gcgatggggt cggcgagccc cagaccagcg
       61 ccgcctgcgc gatccgcgcc cgcgtctcgg cgatcgcgcg aacatgatac ctcggaacgc
      121 gctcttcctt atatgaggtg tcctcctcat gatccacgat gatgagaccc acgtcggcga
      181 gcggagcaaa tacggccaca cgggtaccgg cgacgacttt gaactcgccg gacgcgattc
      241 gctgccagag cttcctgcgc tccggatcac tctgggcgcc gtgatagagc gccaccgaaa
      301 ccccgatctt ccgctcaatc catgcggcca gccgctccgc ctgcgcgaat tctggctcga
      361 gcgcaatggc cccccggccg tgctgaagcg cccagagaag cgcgccggta taggcctcaa
      421 acctggcatc ctcgccgatg atgacgatcc gcgcggctga ttccgcggtg agaagatcga
      481 caacccctgc tccattgccc gaagcatcaa cttctttctc ctgctcccgt tgggaggcag
      541 ggttcggtcg ggaagtcggc agggacgatc gccgggcgga ttgtgccttc ggcgggagca
      601 tcgtatcaat ggcctcgccg accgaacaga catagtggtc ggccatccac gcggcgagtg
      661 tcacaaggtc cgaggggaga accgggagtc gttcatccag cgcggcgacc ggcttgactt
      721 cgcgtcccgc cgttggagac agccctacga cgtatcccga agtcgtctgc tttccaaatg
      781 gtacgcggac tggaatcccg atcgtgactc gacgctcgag cgctacgggg accgagaagg
      841 tgaatgtctt atcgcccgcc cgcagcggag cattgattgc aacttcagca aatacggtca
      901 taggtcgtag gtcctagtgc gtctcccctg tcttccccgt cttccctgtc ttccccgtct
      961 caatgcgtca tggatcaaat tggtctgagg gttagactgg gaagactggg tagacggggt
     1021 ggacggggta gacggttagc gtgcctcgag gattgctgcg atctcctcga ggatggcgtc
     1081 aggttcggac atgcgaccga tcgcctcttt gccggacgca agatggccca cctcgggtcc
     1141 gatcacccgg catccacggc cgcggagcgt tttgaggtgt tcttgagtgg aaggatgttc
     1201 atacatgatg tcgctcatgg caggggcgag caacaccggc gcccgcgtgg ccagtgccgt
     1261 tgcggtcacg acgtcatccg cgagccccag cgcaaacttg gcgatcgtct gggccgtgac
     1321 cggagcaatg accaaaagat ccgctcgttc gccaaggtag acgtggggtt cttcgtacgg
     1381 gttgttcgga tcccacatgt ccgtgatcac ggcgttctgc gacagcgtgc gaaacgtcag
     1441 cggctggacg aactgcgcgg ccgatgctgt catcacgacg tggacgtccg caccttgctt
     1501 gcgcagcgcg ctgatcaggt acgccgcctt gtaggccgcg ataccgccgg tgacgccgac
     1561 gacgatgagc ttcccctgaa gtctgttctg ggccatagat acgctgatct taccgcctct
     1621 tcccggacct cggaccccgg ccggtggacc cccgaagtcg ggttcgtccc cggctcccgg
     1681 ctctagggct ctgcttcctg gcccctggtc tcgctatctt cggcgtcgca cccacgaatc
     1741 cgacgagcgc ctgcgcgatc gcttctgcgc tccacttcgt ggtgtcgatg accaggtgat
     1801 aggggctgag gtcatcgacg ttgatcctat ataactgctc atatcgtgcc cactcactgc
     1861 gctctcgctc caccagctct gcctgcgccg ccgcgaccgg ccgtccctcg cgggcagcca
     1921 cgcgctccgc gcggatctgc gacggcgctt tcaaccacac ctttagatcg gcctcgacca
     1981 tccatcccga gaggcggctc tcgacgacac aatcgccccg ttgcgccagt tcacgctgcc
     2041 gccggtcgac ctcgtggtcg agagaggagt cccgctcggc gagtttgttc acgtcggtca
     2101 gtgagatgcc gcgctgccgg gcgatgtcgt gaaaaacttc gcctccggaa atgtaccggt
     2161 agcccaactg ctgcgccagc agcttcgcga cggtgctctt ccccaccccg atcggccccg
     2221 cgatcgccac gatcactcgg gattctcctg attcccttca cggtcggtaa agcggtgggc
     2281 gacggtctca ggctgcacgg ccgagaggac gacatgaccg ctgtcggtaa tgaccactgc
     2341 ccgcgtgcgc cgcccgtacg tggcgtcgat cagcgttccc cggtcacgcg cttcctgaat
     2401 gatgcgtttg atgggggccg aatcgggcgc gacaatggca atgatgcggt tggccgccac
     2461 gatgttgccg aacccaacgt tgatcagtcg ggggtctgcg aggctattca aggttctgga
     2521 cctgctcccg gagactttcc agttcgccct tgatcgcgat aatagcccgt ccaatctcca
     2581 ggtcgttcgc cttactgccg atcgtgtttg cctcgcggcc catctcttgc agaacgaact
     2641 caagttttcg cccgatggcc ccggcgtggt tgctgaggtc ctcccggaac tgcacgacgt
     2701 ggctgcgaag acgcgtgatc tcctctgaga tgtcgctgcg ctcggcgaag accgccactt
     2761 cggtgaccag gcgttgctca tcgacgggaa tgtggccgag gagctcggtg atcctttgcc
     2821 gtagtcgcgc tgaatagtcc gccaccaccg tggtggcacg gctctcgatt tcctttgcgc
     2881 gcgcttcgat ccgctcgagc ctggtccgca gatcggcggt gagccgcgcg ccctccgctt
     2941 cacgcatgac cacaagctcg ctcagcgcct catccaaacc ctgggagagg agaggccata
     3001 tgccttcgac atcttctggt ggttcttcaa ccttgatgac gtccgggaat gacgcgattt
     3061 gcgggagggt gacctcgcct ccgataccca gcacacgggc gagatctgtg agggcttgag
     3121 tataagcagc ggcgagttcg gtgtcgggcc gaatggcgcg gacgcgggtg gcgcggttgt
     3181 cccgcatgat cgagacttcc acccgacctc gcgacacacg cgactgcaca gttcctcgca
     3241 cgcgctcctc gagcgggctg aggtcgcgcg gcaaacgcac caggacctcg ccaaaacggt
     3301 ggttgatcga tcgaatttcg accagaaagc gg
//
LOCUS       DBNGSB010000109         4136 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_1299563, whole genome shotgun sequence.
ACCESSION   DBNGSB010000109 DBNGSB010000000
VERSION     DBNGSB010000109.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_1299563"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="AC159B_02990"
     CDS             complement(<1..496)
                     /locus_tag="AC159B_02990"
                     /inference="COORDINATES: protein motif:HMM:NF012613.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HIR3376749.1"
                     /translation="MNRFARAWVFFTTIALAAAPAWGAIFSDIQGLPAQRAIERIAAK
                     GVMRVSGERFNPSGTVTRSDFAVFLSRALGISGQGLQPPEVKDWAEVPREAQSAVTAM
                     ASMGSATPQKVEARKGSLVFALQTDKPSYGPTDMVELTFTITNTGSSDAKFEFVNSQF
                     YDFII"
     gene            complement(680..2395)
                     /gene="argS"
                     /locus_tag="AC159B_02995"
     CDS             complement(680..2395)
                     /gene="argS"
                     /locus_tag="AC159B_02995"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HIR3376750.1"
                     /translation="MTEYAFTRVKREALEAARRALGEPVDLPVVLPPAEVAADLAIPC
                     FPLAAQRRKSPPEIAGELARTIQPGPSFAGADAAGGYLNLTLARPAFASSVMGEVLGL
                     GDRYGTNQVGAGHAVVIDLSAPNVAKPMSVGHLRSTIIGAALYRLHGFSGYRPIGDNH
                     LGDWGTQFGALLYAYSQWLDRHAYEQDPIAELLRLYVKFDEEARKDPSLRDRAREWSL
                     RLEQGDPEARRLWEEFVRHSTAEFQRTYDLLEVSFDHTLGESFYEDKTREVIEQAVSR
                     GIAVEDEGALIIRLEGAGISTPVLLRRRDGATLYHTRDLATAMYRIRTFRPAKILYVV
                     GSDQRLHFRQLFAALRELGYGDVEYVHVDFGLVRLPEGRMSTRRGRVVFLQDVLEEAI
                     ERARALVDEKNPELPDSERAAVSRIVGVGAIKYADLSQSRVKNVVFDWDRMLSLEGDS
                     APYLQYTYVRARGIIRKAGEASPGESFDARAAASAQEWALIKHFSRFPEAVHEATHTY
                     FPHVVANYLFQLAQLFHAFYHEIPVLQAEDEDLRRSRRQLVWGAATVMRTGLDLLGIR
                     VPERM"
     gene            complement(2487..3617)
                     /locus_tag="AC159B_03000"
     CDS             complement(2487..3617)
                     /locus_tag="AC159B_03000"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012900411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR3376751.1"
                     /translation="MNGRSTPGALLLLIIAVAVVGGLLGTAVLPRFFSMAGPQSPPQT
                     PPPAASPAPPATNAPPLSAPRVIEREESVVISASERVRPSVVNIDTEVQVQTFFGVFP
                     QKGAGSGVIVSQDGYILTNNHVVEGAQQIKVTLLSGKTLTARIIGTDRFSDLAVLKVD
                     SPDRLPAAALGRSSGLRVGQMAIAIGNPFGLGHTVTVGVISALNRNIQVPNLVIENLV
                     QTDAAINPGNSGGALLDSRGEVVGINTAIVREAQGIGFAIPIDTARAIMDQLIRQGRV
                     VRPYVGIVWGGDVDANIARQYNLPVEHGVIVREVDSGGPAAAAGIQPEDIIISIDGAA
                     VANWNDFIRALFNKRPGDTVRLEAVRSGSRRTFTVRLAERKQ"
     gene            3764..>4136
                     /locus_tag="AC159B_03005"
     CDS             3764..>4136
                     /locus_tag="AC159B_03005"
                     /inference="COORDINATES: protein motif:HMM:NF037651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit beta"
                     /protein_id="HIR3376752.1"
                     /translation="MLNWLRRPKYSSLQRRDMPAGLWAKCPRCSNLIYRKELERNLKV
                     CPKCGFHHRLTAIERLEITLDQDSFEEIDADLTSGDPLKFEGYPQKLQEAREKTGWAE
                     AVITGEGTIDGYRTVIGVLDFH"
BASE COUNT          814 a         1294 c         1274 g          754 t
ORIGIN      
        1 tgatgatgaa atcgtagaac tgagaattga caaactcaaa cttggcgtcg ctcgagcccg
       61 tgttggtgat cgtaaacgtg agctcgacca tgtcggtcgg accgtacgag ggcttgtcgg
      121 tctgcagcgc aaagacgaga gagcccttgc gcgcttccac tttctgaggg gtcgcgctgc
      181 ccatactcgc catcgcggta acggcggact gcgcctcgcg cggcacttcc gcccagtcct
      241 tgacctcagg aggctgcaac ccctgtcctg aaatgccgag cgcgcgggag aggaataccg
      301 caaagtccga gcgcgtcacg gttcctgagg gattgaagcg ctcgccgctc acgcgcatga
      361 cgcccttggc cgcgatccgc tcgattgccc gctgggcggg aaggccctgt atatctgaaa
      421 agatggcgcc ccacgccggc gccgcggcga gcgcgatcgt cgtgaagaat acccaggcgc
      481 gtgcaaaacg attcattcta tgcccctgtt cttggaaaat tgggaaaacc ggacggtggc
      541 tgcggcacct cgaaggatcg ggtgcgccaa aactttgagg ttattaggtt gaatccgcgg
      601 aagggttcgc ttccccggca gctattcctg ccgtccatat cctccagtac gcattatacc
      661 cgggaatggc tccgtgtcac tacatgcgct cggggacgcg gatgcccagc aggtcaagtc
      721 cggtgcgcat gacggtggcg gcgccccata cgagctgccg gcggctgcgc cggaggtcct
      781 cgtcttccgc ctgcagcacc ggtatctcgt gatagaacgc atggaagagc tgagcaagtt
      841 ggaagagata gttggcgacg acatgcggga agtaggtgtg cgttgcctcg tgcacagcct
      901 cggggaagcg cgagaagtgc ttgatcaatg cccactcctg tgcgctcgcc gccgcgcggg
      961 cgtcgaatga ctccccgggc gacgcctcgc ctgctttgcg gatgatcccc cgggcccgca
     1021 cataggtgta ctggagatag ggcgcgctgt cgccctcgag ggacagcatc cggtcccagt
     1081 caaagacaac gttcttcacg cggctttggg agagatccgc gtacttgatc gcgcctacgc
     1141 ctacgatccg agacaccgcg gcacgctcgg aatccggaag ctcagggttt ttctcgtcga
     1201 cgagcgcgcg cgcacgctcg atcgcttcct ctaatacgtc ctgcaggaac accacacggc
     1261 cacgccgcgt gctcatgcgg ccctccggca gccggaccag gccaaaatcc acgtgcacgt
     1321 actcgacatc gccgtatccg agctcacgca gtgcagcaaa gagttgccgg aagtggagtc
     1381 gctggtcaga tcccacaaca tacaggatct tggccggacg gaacgtgcga atgcggtaca
     1441 tcgccgtcgc cagatcccgg gtgtggtaaa gtgtcgcacc gtcgcggcgc cgcagcagca
     1501 caggcgtgga gatgccggcc ccctcgaggc ggatgatcaa cgcaccttcg tcctcgacgg
     1561 cgatgccgcg cgagactgcc tgctcgatca cctcgcgggt cttgtcctcg tagaaactct
     1621 cgcccagcgt gtgatcgaag ctgacctcga ggaggtcata ggtccgctga aactcggccg
     1681 tgctgtgcct gacaaattcc tcccagaggc gccgggcttc aggatccccc tgttccaggc
     1741 gcagtgacca ctcgcgcgcc cgatctcgta gggacggatc ttttcgcgct tcctcatcga
     1801 acttgacgta caaccgaagc agttccgcga ttggatcctg ctcgtaggca tgccggtcca
     1861 gccactggga ataggcatag agcagggccc caaactgtgt gccccagtct ccgagatggt
     1921 tgtcgccgat cgggcggtag cccgagaacc cgtgcagcct gtagagggcg gccccaatga
     1981 tcgtggagcg taagtgtcca acggacatcg gcttggccac gttgggtgcc gaaaggtcga
     2041 tcacgaccgc gtggcccgca ccgacctggt tggtgccgta gcgatcgcca agcccgagaa
     2101 cctcgcccat gacggaggag gcaaacgcag ggcgcgccag ggtgaggttg aggtaacctc
     2161 ctgccgcgtc tgcacccgcg aacgatggtc caggctggat agtccgggcg agttcgcccg
     2221 cgatctctgg aggggatttc cggcgctgcg ccgcgagcgg aaagcacgga atcgccaggt
     2281 cggcggccac ttcagcgggg ggcagaacca ccggcaggtc gacgggctct ccgagcgcgc
     2341 ggcgggcggc ctcgagcgcc tctcgcttga cgcgcgtaaa cgcatactcg gtcatgcgaa
     2401 tattatacga agctgaagag ctgaaaggct ggagagcgac tgcttgaact ctccagcttt
     2461 ccagctttcc aactctccaa ctaattctat tgctttctct cggcaagcct tacagtaaag
     2521 gtccggcggg agccgctccg taccgcctcc aaccgtaccg tgtctccggg ccgtttgttg
     2581 aagagcgcgc ggatgaagtc gttccagttg gccacggccg cgccgtcgat cgagatgatg
     2641 atgtcctccg gttggatacc ggcagcggca gccgggccgc cggaatccac ttctcgaacg
     2701 atgacgccgt gctccaccgg gagattgtac tgcctggcga tgtttgcatc cacgtctccg
     2761 ccccatacga tgccgacgta cggccgcacc acacggccct ggcggatcag ctggtccatg
     2821 atggcccgcg ccgtatcgat cgggatcgcg aacccgatac cctgcgcttc gcggacgatc
     2881 gcggtgttga tgccgacgac ctcgccccgg ctgtcgagga gcgcgcctcc gctgttcccg
     2941 gggttgatcg cggcgtcggt ctgcacaagg ttttcaatga ccagattggg cacttggatg
     3001 ttacggttca gcgcgctgat gacccccact gtgacggtgt gcccgaggcc aaaaggattc
     3061 ccgatggcga tggccatctg acccacgcgc agtccactcg aacggcccaa tgccgccgcc
     3121 ggcaaacggt caggtgagtc caccttcagc acagccagat ccgagaatcg atcggtccca
     3181 atgatccggg cggttaacgt ctttcccgac agcagcgtca ccttgatctg ctgcgccccc
     3241 tccacgacgt gattgttggt aaggatgtaa ccatcctgac tcacgatgac accagacccg
     3301 gcgcctttct gtggaaacac accgaagaat gtctgcacct gaacctccgt gtcgatgttg
     3361 acgacggaag gacggacgcg ctcagaggca ctgatgacga ccgactcttc tcgctcgatg
     3421 accctgggcg cgctcaacgg tggagcgttg gtcgctggag gcgccggcga cgcagcaggg
     3481 ggcggtgtct gtggcggact ctggggtccg gccatcgaga agaacctcgg gagaaccgca
     3541 gttcccagta acccgccgac gacagcaacc gcgatgatca gaaggagcaa cgctccgggc
     3601 gtggaccgtc cgttcattta ccttcaccgt cctttgaaaa cacgtgactt caattataga
     3661 actgcgggaa cacagggaac atgggcatca cagggaagat ggcatacgct ataatcgaaa
     3721 aaacgcattc ctatcttccc cactttggag gggtgcgaac gccatgctga actggctccg
     3781 gcgccccaag tacagttccc tgcagcggcg cgacatgccc gccggtttgt gggcgaaatg
     3841 cccacgctgc agcaacctca tctaccgcaa agagctggag cgaaatctca aggtctgccc
     3901 caagtgtggc ttccaccatc gtttgacggc gattgagcgt ctggagatca cccttgacca
     3961 agacagcttc gaagagattg acgccgatct gacctccggg gaccccctga agttcgaagg
     4021 gtatccacag aaactgcagg aggccaggga gaaaacaggc tgggcggagg ctgtgatcac
     4081 cggcgaggga acgatcgacg ggtaccgcac cgtcatcggc gtgctggact tccact
//
LOCUS       DBNGSB010000110         4563 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_993723, whole genome shotgun sequence.
ACCESSION   DBNGSB010000110 DBNGSB010000000
VERSION     DBNGSB010000110.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4563)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_993723"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="AC159B_03010"
     CDS             <1..223
                     /locus_tag="AC159B_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376753.1"
                     /translation="NSLISAIGANDSVRAAVEAHGDTAYQSRLILPLLRQSLHPSRIT
                     ATSGAMLKALRYGLRALWDDGAHLARSRS"
     gene            365..1642
                     /locus_tag="AC159B_03015"
     CDS             365..1642
                     /locus_tag="AC159B_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HIR3376754.1"
                     /translation="MRIIADLHLHSKYSRATSREMDVENMARWCKIKGITIVGTGDFT
                     HPVWFRELKAKLKPTDYGLYTFGGVHFMLTVEVSNIYPQGGRLRKIHNIIFAPSLEVV
                     ERINAVLGRFGSLMADGRPTLTLPSDKLVEYIMEISADCMIVPAHAWTPWFSLFGSNS
                     GFDSLRECFRDESDHIAAVETGLSSDPAMNWRLSQLDAVMLLSNSDAHSPAKLGREAN
                     VFEIGEPDYHELVRIIREKDTSKFLYTIEFFPEEGKYHYDGHRNCNTRLTPKESKAAG
                     NRCPVCKRPVTVGVMHRVEDIADREDGYVPSGAVPYRNLIPLEEIVAEAKGAQVGTGA
                     VREEYFKLCARLGSEFSVLLDVPLEELTKHTSSRIAEGIRRVREGQVQIKPGYDGAYG
                     EIHIFGDAGPTDTRLEEVEEEKKTEQPAQTTLF"
     gene            1742..2914
                     /locus_tag="AC159B_03020"
     CDS             1742..2914
                     /locus_tag="AC159B_03020"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HIR3376755.1"
                     /translation="MASVILKPGKESRLARGHLWVYAGEIANIDGHVDDGGIVDVRSL
                     RGQWLGRGFINRRSTLTVRLLTTRPEEIGREFFHKRLADAIAYRKRIVHGTSAYRLVY
                     GEGDLLPGLILDRYGDIAVLQTLALGMDVRKPTLVDLINEMLRPSAVYERNDPGVRRL
                     EGLPAQTGWLSGEAPTEMEVQEGPAAFLIDIARGQKTGFFLDQRENRLAIEQLVKDAD
                     VFDAFCYTGAFSVHAALGGAKHVTAVDISEEAITLARENARRNGVAERCTFAVANVFD
                     ELRRQVSTGARYDVVILDPPAFAKTKESLPRAVAGYKEINLRALKLLRPGGILVSCSC
                     SYHVYEALLIEVLASAASDAHRTVRLMERRGQAQDHPVHPAMPETRYLTCLIAHVT"
     gene            3056..>4563
                     /locus_tag="AC159B_03025"
     CDS             3056..>4563
                     /locus_tag="AC159B_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HIR3376756.1"
                     /translation="MAPRQIQITDNLDLLLEVLPPHLRESIEEQNNQETLLEIVIDLG
                     RKPEARYPGRVVSLNEGYVSHEDIGFVTSHVGAFGKDNRAGIERTLHRISAIRNRQGE
                     IIGLTCRVGRAVVGTVDIVRDVIESGKSILLVGRPGVGKTTLLREAARVLADEFGKRV
                     VVVDTSNEIAGDGDIPHPGIGAARRMQVPHPELQHAVMIEAVENHMPEVIVIDEIGTE
                     AEALAARTIAERGVQLIATAHGNTLDNLLQNPTLSDLVGGIQAVTLSDEEARRRGTQK
                     TGLERKAPPTFDVVIEIQEKDKLAVHHEVSVVVDRFLRGAPPRPETRVRTQEGEVQIE
                     APAVAPRADRWTPQGPMAPPPSEDATRPGRKVTRIYPYAVSRDRLERAIRELRVPAYV
                     SDNLADADVVLTLKSQERRQPKKLRDAVARGRTMHVLKSNTINQIQGFLRSIFEVEDL
                     DEQEVALREVEEAINEVLTTTQPVELSPQNSYVRRLQHQLIQRYGLASESRG"
BASE COUNT          921 a         1394 c         1410 g          838 t
ORIGIN      
        1 caacagcctt atctccgcga tcggtgcaaa cgactctgtc cgtgccgcgg tcgaggctca
       61 tggggatact gcctaccaat cgcgcctgat cctgcctctc ctgcggcaat cgttgcaccc
      121 gtcgcgcatc acggccacgt ccggagcgat gctgaaggcg ttgcggtacg gactgcgagc
      181 cctctgggac gatggcgccc acctggctcg ttcgcgctcc taaaggagat gattccgatt
      241 aacgtgaacc gatacgcaga tcaggggccg ggggtcgggc aaggggagcc gtaactaagt
      301 cactccacag ccgctctgcc ggctccccgg tcccggcccc ggctcctgat ctctgagaca
      361 caccatgcgg atcatcgccg acctccacct ccactccaag tacagcaggg cgacaagccg
      421 ggagatggat gtggagaaca tggcgcggtg gtgcaagatc aagggcatca ccatcgtcgg
      481 taccggcgac ttcacccacc ccgtctggtt ccgcgaactg aaagccaagc tcaagcccac
      541 ggactacggt ctgtacactt tcggcggtgt gcattttatg ctgaccgtcg aggtctcaaa
      601 tatctacccg cagggcggcc ggctccggaa gattcacaat attatcttcg cgccatcact
      661 cgaggtcgtg gagcgcatca acgcggtcct gggacggttc gggtcgctga tggccgatgg
      721 gaggcccacg ctgacactcc cgtcggacaa gctcgtcgag tacatcatgg aaatctcggc
      781 cgattgcatg atcgttcccg cgcacgcctg gaccccttgg ttcagcctgt ttggatcaaa
      841 ctcaggcttc gattcattac gcgagtgttt ccgtgacgaa tcggatcaca tcgccgccgt
      901 tgagaccggg ttgagcagcg atccagcgat gaactggcgg ctctcacagc ttgatgcggt
      961 gatgctcctc agcaactccg atgcgcattc ccccgcaaag ctgggccgcg aggcgaatgt
     1021 ctttgaaatc ggcgagcccg attaccatga gctcgttcgt atcatccgcg agaaggacac
     1081 gagcaagttc ttgtacacga ttgagttctt tcctgaagaa ggtaaatacc actacgacgg
     1141 acataggaac tgcaacaccc gcctcacgcc aaaagagtcc aaggcggccg gcaaccgctg
     1201 ccctgtctgc aaacggccgg tcacggtcgg ggtcatgcac cgggtcgagg atatcgccga
     1261 ccgcgaggat gggtatgtcc cttcgggcgc ggtcccgtac cgtaatctga ttccgctcga
     1321 ggagattgtc gctgaagcca aaggcgcgca ggtcggcacc ggcgcggtcc gggaagaata
     1381 cttcaagctg tgcgcgcggc tgggcagcga gttctcagtg ctgctcgatg ttcctttgga
     1441 ggaactgacg aagcacacgt cgtcccggat cgccgaggga attcgccgcg tccgtgaagg
     1501 ccaggtgcag atcaaaccag gctacgacgg tgcgtatggc gagattcaca tctttgggga
     1561 tgctggcccc accgatacgc gcctcgaaga ggtcgaggaa gaaaagaaga ccgagcagcc
     1621 tgcccaaaca accctattct aacgaccgga agcgcggaaa cgcggaagcg cggaaactta
     1681 atgcacccat gtttgattcg ttacgtttcc ccgtttccgc gtttccctgt ttccgtccgt
     1741 tatggctagc gtaattctca aacccggcaa agaatcgcgc ttggcccgcg gccatctctg
     1801 ggtctacgcc ggggagattg ccaacattga cgggcacgtg gacgacggcg ggatcgtcga
     1861 cgtccgttcg ctgcgaggac agtggctggg caggggattc atcaaccgca ggtccacact
     1921 caccgtccgg ctcctgacga cacgtcctga agagatcggc cgcgagtttt ttcacaagcg
     1981 gctggcggat gcgatcgcct atcgcaaacg aatcgttcac ggaacatccg cgtaccggct
     2041 cgtctacggc gagggtgatc tgttgccggg actgatcctc gatcgttacg gcgacatcgc
     2101 ggtcctccag acgctcgcgt tggggatgga cgtgcgtaaa ccaaccctgg tcgacctgat
     2161 caacgagatg ctgcgcccgt cggctgtcta cgagcgcaac gacccaggcg tccgcaggct
     2221 ggaaggcctg cccgcgcaga cggggtggtt atctggtgag gcgccgacag agatggaggt
     2281 ccaggagggg cctgccgcgt tccttatcga catcgcccga gggcagaaga ccggattctt
     2341 tctggatcag agagaaaatc gcctcgcgat cgagcagttg gtcaaggacg ccgatgtctt
     2401 cgatgcattc tgctacacgg gggcgttctc cgtccacgcg gcactcggcg gcgcaaagca
     2461 cgtgacggcg gtcgacatct ctgaagaggc gatcaccctt gccagagaga acgcccggcg
     2521 caatggcgtg gcggagcgct gtacgtttgc ggtcgccaac gtcttcgacg agctgcggcg
     2581 gcaggtcagt acaggcgcaa ggtacgacgt cgtgatcctg gatccaccgg cctttgcgaa
     2641 gaccaaggaa tctctccccc gggcggtcgc aggatacaaa gagatcaacc ttcgcgcgct
     2701 caagctcctc cggcccggcg ggattctggt gagttgttca tgctcgtatc atgtctacga
     2761 agcgctgctc attgaggtgc tggcgagcgc cgcgagcgac gcgcatcgca cggtgcgact
     2821 gatggaacgc cgcggacagg cgcaagatca ccccgttcat ccggccatgc ctgagacgcg
     2881 ttacttgacc tgcctgattg ctcacgtcac gtgagaaaag ggtcgccaga aacgggtatg
     2941 gtataattga tgcgatgttc ctcgttttgt tgttcgattc cgttacccgc aatttctata
     3001 ggtgagcgcc ctgcctgacg ccttcgctcg agttcttgca gaaggagagc ggtccgtggc
     3061 accccgacag atccagatta ccgacaatct ggatttactg ctcgaggtgt tgcctccgca
     3121 tctgcgggaa tccatcgaag agcagaacaa ccaggaaacc ctcctggaga tcgtgatcga
     3181 tctgggtcgc aaaccagaag cgcgctaccc cggccgtgtg gtttccttga atgagggcta
     3241 cgtgtcccat gaagacatcg gctttgtcac cagccacgtg ggggcgtttg gaaaagacaa
     3301 ccgggcgggc attgagcgca cactccatcg catttcggcg atccggaacc ggcagggcga
     3361 gatcatcggc ctcacctgtc gcgtcggccg cgccgtcgtc ggaaccgtgg acatcgtgcg
     3421 tgatgtcatt gagtccggta agagcattct cctggtcggt cggcccggcg tggggaaaac
     3481 gacgctcctg cgcgaggcgg cccgtgtcct cgccgatgag ttcggtaagc gcgtggtcgt
     3541 cgtcgacacc tccaatgaga tcgccggcga cggggatatt ccacatccgg gtatcggcgc
     3601 cgcccggcgc atgcaggtgc cgcatcccga gctgcagcat gcggtgatga tcgaggcggt
     3661 cgagaaccac atgcctgagg tcatcgtcat cgacgagatc gggacagagg ccgaagcgct
     3721 ggcagcgcgc acgattgccg agcgaggagt gcagttgatc gcgacggctc atggcaacac
     3781 gctggacaac ctgctgcaga atccaaccct gtcagacctt gtgggcggaa tccaggccgt
     3841 cacgctctcc gatgaggaag cccgcaggcg cggcactcag aagaccggcc tggaacgcaa
     3901 ggcgccgccg acgtttgatg tcgtcatcga gatccaggaa aaggacaagc tggccgtgca
     3961 tcacgaggtc tccgtcgtcg tggatcgatt cttgcgcggc gccccgccgc gcccggagac
     4021 ccgcgtccgc acccaggaag gcgaagtgca aatcgaggcc ccggcggttg ccccgcgggc
     4081 ggaccggtgg acgccgcagg gcccgatggc cccgccgccc tctgaggatg caacgcgacc
     4141 cggtcgcaag gtgacgcgaa tttatcctta tgcggtgagc cgggaccgcc tcgagcgggc
     4201 gatccgagaa ctgcgcgtac cggcgtatgt ttccgacaac ctggccgatg ccgatgtggt
     4261 gttgacgctc aaatcacagg aacgcaggca accaaagaag ctgcgcgacg ccgtggcccg
     4321 cggccggacg atgcacgtgc tgaagagcaa caccatcaat cagatccagg ggttccttcg
     4381 aagcatcttc gaggtggaag acctcgatga acaagaggtc gcgctccgcg aagtcgagga
     4441 agccatcaac gaagtcctca cgaccacgca gccggtcgaa ctatccccgc agaactcata
     4501 tgtccgccgc ctccagcacc agctcatcca gcgttatggg ctggcatcgg agagccgtgg
     4561 cga
//
LOCUS       DBNGSB010000111         5046 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_227576, whole genome shotgun sequence.
ACCESSION   DBNGSB010000111 DBNGSB010000000
VERSION     DBNGSB010000111.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 5046)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5046)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5046
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_227576"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="AC159B_03030"
     CDS             <1..806
                     /locus_tag="AC159B_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013682133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HIR3376757.1"
                     /translation="FTVAMLETWEELASKPYGMILVSGPTGSGKTTTLYSTLNKINSL
                     EKNILTVEDPVEYQLGRVNQVQINPKAGLTFASGLRSFLRQDPDIIMVGEIRDRDTAE
                     IAIQASLTGHLVLSTIHTNDAPSATTRLMDMGIEPFLITSSLTGVLAQRLARTICAHC
                     KESYAPPVEALHRLGLAPESGEEIVFYRGKGCDRCKGSGYKGRTGLFELMVISEPIRE
                     LVLKGVSANEIREQAISEGMRGLREDGILKVLEGVTTVDELLRVVFVDV"
     gene            823..2520
                     /locus_tag="AC159B_03035"
     CDS             823..2520
                     /locus_tag="AC159B_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012652.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HIR3376758.1"
                     /translation="MSESFQGKRQPIGEMLHAEGVITRQQLTRALEIHHASGERLGRV
                     LLDMGAVDQEHVARVLSQQVGAEFVRLTGRRIPEDALRLLSPTDAGRLRAIPIAHDDG
                     WLTVAMVDPLDVVAVDDIRRHTGLKVRIVVTTVADFQHALDQYPRGDDPMAATIRDLP
                     LPAIADEPVSVDWLRRAAEEQPIVRLVSQMVEQAVRHRATDVHLEPQDHDVRIRYRID
                     GMLKQTHHLPKHVHNRVVSRIKILASLDIAERRMPQDGSFQTRVDGRAVDVRVATLPA
                     FFGEKVVLRLLDKAGAIHVLDELGIAHATLTRLRRITARPQGLFLLTGPTGSGKTTTL
                     YAVLNEINNESVNIVTIEDPVEYQIPGVTQVQVNPRAGVTFPSALRHVLRQDPNIILV
                     GEIRDEETARIATQAALTGHIVLSTLHTVDAMGAVTRLLDMSIQPYLVVASLAGVAAQ
                     RLARRLCPKCKQADLLSLEDLRLRFGEDVPEGEYCLPRGCEFCNNTGYRGRAALFELA
                     EMTEDIRYLVINNQPAHMLKERAGGEGMTTLLRDGLLKAAAGITSLAEVERVVSVEV"
     gene            2611..3777
                     /locus_tag="AC159B_03040"
     CDS             2611..3777
                     /locus_tag="AC159B_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HIR3376759.1"
                     /translation="MNIDDLLRDTLNAKASDLHLTTGIPPMIRVWGKLSPLNHPALTP
                     EDTFQLAYGMLNTFQKQKFEKNWELDLSYAVEGLGRFRVNVYRQRGSVGVACRTISET
                     IPSIEELNIPMVLKDLCRLPRGLILVTGPTGHGKSTSLAAMLDFINTERSSHIVTIED
                     PIEYMHRHKKSMVNQRELGFDTQTFPNALRAVLREDPNVIMVGEMRDLETIGAALTIA
                     ETGHLVFATLHTANAAQSIDRIVDVFPPHQQQQIRVQLSGVLEAVISQQLLPNLQFVD
                     RKERRPVAAAAIVRTSTGGDGRGRPHWPTLEELGRVPAVEMMIATPGIRNLIREAKTH
                     QIHTAIQTGAQYGMQTMDQSLHALFSRRLVSLENALARAIYPDELRKMIERGGQ"
     gene            3781..4992
                     /locus_tag="AC159B_03045"
     CDS             3781..4992
                     /locus_tag="AC159B_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR3376760.1"
                     /translation="MATFRYSARDGAGRVVAGAIEAETEVGVIGKLQEMGFFVTSLER
                     EQQRTSLRATLRRMRKVGMRELTVFMRQFATMVNAGLSMVRTLTILEQQAENQKLKEI
                     VALLRKDVEEGTTLSDAFGKHPQTFSGLVINMIRAGEIGGVLDDVLNRIAIFFEKDLA
                     LRQKVRAAITYPTAIFIFALGIIFFLVFFILPQFIGFFEGLDLVLPLPTRIMITFTNV
                     ATGYWYVFLGLMGLGFYALRTYVRTTAGRFAFDRWKLRIPVFGALLRKVTVSRFTRTL
                     GTLISSGVPILQALEVVAKAVDNKVVAQAIENVRSSIREGESIALPLQSSGLFPPMVV
                     QMTAVGEETGTLDTMLQKVADFYDAEVETTLAQLTSILEPLLIMFLGFVVGFIVLAFY
                     MPLYQLITGIK"
BASE COUNT         1007 a         1557 c         1563 g          919 t
ORIGIN      
        1 ggttcacggt cgcgatgctg gagacgtggg aggagctcgc aagcaagcca tacgggatga
       61 ttcttgtcag cggccccacc ggcagcggaa aaacaacgac cctttactcg accctgaaca
      121 aaatcaattc gctcgagaag aacattctga cggtagagga tcccgtcgag taccagctgg
      181 gtagggtgaa ccaggtccag atcaacccca aggcagggct gacctttgcc agcggcctcc
      241 gttcgttcct gcggcaagac ccggatatca tcatggtcgg cgaaatccgc gaccgggaca
      301 cggcggagat tgcgatccag gcgtccctca ccggccactt ggtgctctcc acgattcaca
      361 ccaacgatgc acccagcgcc acgacccgcc tgatggacat gggcatcgag ccgttcctta
      421 ttaccagctc gctgaccggc gtactcgcgc agcggctcgc ccggacgatt tgcgctcact
      481 gcaaagaatc ctacgcgcct ccggtcgaag cgctgcaccg cctcggcctg gcccccgagt
      541 ccggcgagga gatcgtgttc taccgtggca agggctgcga ccgatgcaag gggtcggggt
      601 acaagggccg caccggcctc ttcgagctca tggtgatcag cgaaccgatc cgcgagctgg
      661 tgctcaaggg ggtttcggcc aacgagatcc gggagcaagc aatctcggaa ggcatgaggg
      721 gcctgcgcga agacggcatt ttgaaggtcc tggaaggtgt gacgaccgta gatgaactcc
      781 tgcgcgtcgt gttcgtcgac gtctgagaat tagccgcacg cgatgtccga aagtttccag
      841 ggtaaacggc agccaatcgg cgagatgctg cacgctgaag gcgtgattac gcgccagcag
      901 ctcacccgcg cacttgaaat ccatcacgcc agcggcgaac gccttggacg cgtccttctg
      961 gacatgggtg ccgtcgatca ggagcacgtg gctcgagttc tcagtcagca ggtcggggcc
     1021 gagttcgttc gtctcaccgg tcgtcgaatc cccgaggacg cgctgcggtt gctttcgccg
     1081 accgatgccg gccggctgcg ggccattccc atcgcccatg acgacgggtg gctcacggtt
     1141 gccatggtcg atccgctgga cgtcgtcgct gtggacgaca tcaggcggca cacgggcctg
     1201 aaggtccgga tcgtcgtgac gacggtcgcc gatttccaac atgcgctcga ccaatatcca
     1261 aggggcgacg acccgatggc ggccacgatc cgtgacctcc cgcttcccgc gatcgccgac
     1321 gaaccggtgt ctgtcgactg gctgcgccgg gcggcggaag agcagcccat cgtccgtctc
     1381 gtgagtcaga tggtcgaaca ggcggtccgg caccgcgcca cagacgttca cctcgaaccg
     1441 caagatcacg acgtccgcat ccgctaccgg atcgacggca tgctcaagca aacgcatcat
     1501 ctgcccaagc acgttcataa ccgtgtcgtg tcacgcatca agatcctggc cagcttggac
     1561 atcgcggagc gcaggatgcc gcaggacggc agcttccaga cccgcgtcga cggtcgcgcc
     1621 gtcgacgtca gggttgccac cttaccggcg ttcttcggtg agaaagttgt gctgcggctg
     1681 ttggacaaag ccggggcgat ccatgtgctc gatgagctcg ggattgccca cgccaccctg
     1741 acgcggctgc gtcgcatcac ggcgcgaccg cagggactat ttcttctgac gggtcccact
     1801 ggcagcggca agacgaccac gctgtacgcg gtcctgaacg agatcaacaa tgaaagtgtg
     1861 aatatcgtca cgatcgagga tcccgtcgag taccagatcc cgggggtcac acaggtgcag
     1921 gtgaacccga gggccggggt gacgttcccc tccgcgttgc ggcacgtgct gcgacaggat
     1981 cccaacatca tcctcgtcgg cgagatccgc gacgaggaga cggcgcgcat cgcgacccag
     2041 gccgccctca cgggccacat cgtcctcagc acgctgcaca cggttgatgc gatgggcgcg
     2101 gtcacacgct tgttagacat gagcattcaa ccttacctgg tcgtcgcgtc gctggcgggt
     2161 gtggcggcgc agcgcctcgc gcggagactc tgcccgaagt gcaagcaggc cgacctgctg
     2221 agcctcgaag acctaaggct tcggttcggt gaggatgtgc ccgaggggga gtattgcctg
     2281 ccgcgcggct gcgagttttg caacaacacc gggtatcgag gccgggccgc cctctttgag
     2341 ctcgcggaga tgaccgagga catccgctat ttggtcatca acaaccagcc cgctcacatg
     2401 ctcaaggaac gcgcaggagg cgaaggcatg accaccctcc tgcgagacgg gttgctcaaa
     2461 gcggccgcgg ggatcacctc actggctgag gtcgaacgcg tggtgtctgt ggaggtctga
     2521 aatgtgcttg agaagggctt cttatacgtg gatgcgagcc gtgtggccta tggacaactt
     2581 cacaggggca tgggagaggg cagggacgac atgaacattg atgatctctt gagagatacc
     2641 ctcaacgcga aggccagcga ccttcatctt acgactggga tcccgcccat gattcgcgtc
     2701 tggggcaagc tgtcgccgtt gaaccacccg gccttgaccc cggaggatac gttccagctc
     2761 gcctacggca tgctgaatac cttccagaag cagaagttcg aaaagaactg ggaactggac
     2821 ctctcatacg cggtcgaggg cctaggcagg ttccgcgtca acgtgtaccg tcagcgaggc
     2881 tcggtcggcg tggcgtgccg gaccatctcc gagacgattc ccagcatcga agaactcaat
     2941 atcccgatgg tgctgaagga tttgtgccgg ctgccgcgcg gattgattct cgtcaccggg
     3001 ccgaccggac atgggaaatc gacgtcgctc gcggcaatgc tggacttcat caacaccgag
     3061 cgctcgtcgc acattgtcac gatcgaagat ccaatcgagt acatgcaccg ccacaagaag
     3121 agcatggtca accagcgcga gctcgggttc gacacgcaga cgtttcccaa cgccctgcgg
     3181 gcggtcctgc gcgaggaccc caacgtcatc atggtcggcg agatgcgcga cctggagacg
     3241 atcggcgcgg cgttgaccat cgccgagacc ggccacctgg tgttcgccac gctgcacaca
     3301 gccaacgccg cgcaaagcat cgaccgcatc gttgacgtct ttccgccaca ccagcagcag
     3361 cagatcaggg tacagctttc cggagtgctg gaagccgtga tcagccagca gcttctgccc
     3421 aacctgcagt tcgtcgatcg gaaagagcgg cggcctgtgg ccgcggcggc gatcgtgcga
     3481 acgtccaccg gcggcgacgg gcgaggccga ccccactggc cgaccctcga ggagctgggc
     3541 cgcgtgcctg cggtcgagat gatgattgcc acgcccggga tccgaaacct gatccgtgaa
     3601 gccaaaacgc atcagatcca caccgccatc cagaccggcg cgcagtacgg catgcagacg
     3661 atggaccaat cgctgcacgc gcttttctct cgacggctgg tcagcctgga gaacgccctg
     3721 gccagagcca tctatcctga cgagctgcgc aagatgatcg aacggggagg ccagtaatca
     3781 atggcaacat tccgttacag cgcccgggat ggtgccggcc gtgtcgtcgc cggcgccatc
     3841 gaggcggaaa ctgaagtcgg ggtgatcggc aaactccagg agatgggatt cttcgtcacc
     3901 agcctggaac gcgagcagca gcggacctca ctgcgtgcca ccctgcgccg catgcgcaaa
     3961 gtcggaatgc gggagctcac cgtcttcatg cgccagttcg cgacgatggt caacgccggc
     4021 ctgtcgatgg tgcgtacact gacgatcctg gagcagcagg cagaaaacca gaaactcaag
     4081 gagatcgtcg ccctccttcg caaggacgtc gaggaaggca cgacgctttc ggatgcgttc
     4141 ggcaagcacc cgcagacatt ctcggggctc gtcatcaaca tgatccgcgc gggcgagatc
     4201 ggcggcgtgc tggacgacgt cctcaaccgg atcgccatct tcttcgagaa ggatctggcg
     4261 ctccggcaga aagtgcgggc ggccataacc tatcccacgg cgatcttcat ttttgcgctc
     4321 ggtatcatct tcttcctagt gttcttcatt cttccgcagt tcattgggtt ctttgagggt
     4381 ctagacctcg tgctgccgct tccgacccgt atcatgatca cctttacgaa cgtggccacc
     4441 gggtactggt acgtgtttct ggggctcatg gggctgggct tctacgcgtt gcggacttac
     4501 gtccgcacca ccgccggtcg gtttgccttc gatcgatgga agctgcgcat tccggtcttc
     4561 ggggccctgc tgcgcaaagt aacggtttcc cggtttacgc ggaccctggg aacgctgatc
     4621 tcgagcggcg tgccgatcct gcaggcgttg gaggttgtcg ccaaagcggt cgataacaaa
     4681 gtggtcgccc aggccatcga gaacgtccgg tcaagcatcc gggagggcga aagtatcgcc
     4741 ttgcctctcc agtccagcgg tctcttcccg ccgatggtcg tgcagatgac cgcggtgggc
     4801 gaggagaccg ggacgctcga tacgatgctg cagaaagtgg cagacttcta cgatgcggaa
     4861 gtggagacga cacttgcaca gttgacttcg atcctagaac cgctgctgat tatgttcctg
     4921 ggcttcgtgg ttggattcat cgttcttgcg ttctatatgc cgctgtatca actgatcaca
     4981 ggaatcaagt agcgacaacg ggacaacagg tcaacgggac aactcagatt tagcagtgca
     5041 ccagga
//
LOCUS       DBNGSB010000112         2999 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2221619, whole genome shotgun sequence.
ACCESSION   DBNGSB010000112 DBNGSB010000000
VERSION     DBNGSB010000112.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2221619"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="AC159B_03050"
     CDS             complement(<1..865)
                     /locus_tag="AC159B_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007202830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3376761.1"
                     /translation="MEQVIVDVQDLVKRYGAITAVDHVSFQVNRGEIFGILGPNGAGK
                     TTTLEMIEGLRVPDEGTAVVDGIDIRRDPRGVRALVGVQLQEAGFFDRLTVEETLQLF
                     GSFQRKMLPPAQVIDRMQLQDKRRAWVMNLSGGQRQRLAIAVALINDPRIIFLDEPTT
                     GLDPQARRNLWDVIERFREEGRTVVLTTHYMEEAQRLCDRVAIMDHGRIVALNPPRAL
                     IRTHAPSTTVALTVLEGDLAYASLPGVRRAEVSNGEVVLATSDPMQTVQALMESSRAG
                     QIGFHQLRVEEA"
     gene            957..1472
                     /locus_tag="AC159B_03055"
     CDS             957..1472
                     /locus_tag="AC159B_03055"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3376762.1"
                     /translation="MTFEIRRATARDIKAILPVWGELAGFHASLDPAFTPSPEWPREY
                     GAYLKTLMAREDALALVAKEDLEVIGYAVGRITTLPPFFEHRSRGYIHDVYVREEFRR
                     RGLGRRLVEEILVWFDRRGVTLVELTVAANNAEAIPFWKRLGFFVYMHQMKKEIREQR
                     MGNGERRSRSR"
     gene            1476..2612
                     /locus_tag="AC159B_03060"
     CDS             1476..2612
                     /locus_tag="AC159B_03060"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR3376763.1"
                     /translation="MRIDKVQAAAQNTGLDGFIISTPANTYYASGFRAITYSRPVLVL
                     VSDRPALIIPELEETHARQRSAVKDIRTYSDMALGGLGGKSTLQLAIDLLVEVLHERE
                     LKGRRLGFEPTGFSLEGYNYLSGAWKARLEPTKGIVERLRMVKDDDELRLIRIGAELA
                     DIGMQVESDASVPGATEIEILAKGNAAMLMQASKRYPDLPITAGSRPVSGAKTVLPHS
                     IPSGKPLARGDVVIHGTGCIVDGYYSEDERTIFVGDPTVEQRKLFDVMLNAQQSAIDA
                     IRPGVPCKNVDRAARQVIEDAGYGKTFIHRTGHGIGIDIHELPFFAASDDTVLEPGMV
                     MSVEPGIYVEGKGGFRHSDTIIVTEDGCDVLTKHPKTLPELIFT"
     gene            2623..>2999
                     /locus_tag="AC159B_03065"
     CDS             2623..>2999
                     /locus_tag="AC159B_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HIR3376764.1"
                     /translation="MIGRDRVLSAIAGRSTDRVPVAFWRHFPGEDQRAESLAAAHVAF
                     QKKFDWDFLKVTPASGYYGDDWGLRASYKPNPAGTRQYTDRPIKKPGDWSHLKPLDVT
                     AGVYGRELHALRLVRDALPQLLVL"
BASE COUNT          581 a          940 c          941 g          537 t
ORIGIN      
        1 tggcttcctc gactcgcaac tggtggaaac caatctgccc ggcgcggctg gactccatga
       61 gcgcctgaac cgtttgcatc gggtcgctcg tggcgagcac gacctcgccg ttactgactt
      121 ctgcgcgccg cacacccggc aatgacgcgt acgccaggtc cccttccaga acagtcaggg
      181 ccaccgtggt gctgggcgca tgcgtgcgga tgagggcccg cggggggttg agcgcgacga
      241 ttcggccgtg gtccatgata gccacccggt cacacaggcg ctgcgcttct tccatataat
      301 gggtggtcag cacgacggtc cggccttcct cacggaatcg ctcgatgaca tcccagagat
      361 tgcgccgcgc ttgagggtcg agcccggtcg tgggttcgtc cagaaaaata atgcggggat
      421 cgttgatcag ggcgaccgcg atcgccagtc tctggcgctg gccgcctgac agattcatca
      481 cccacgcgcg ccgcttgtcc tgcagctgca tccggtcgat gacttgggcc ggcgggagca
      541 tcttgcgctg aaacgacccg aacaactgca gcgtctcctc gaccgtcagc cggtcgaaga
      601 accccgcctc ctggagctgg acaccgacga gagcccggac cccgcgcgga tcgcggcgga
      661 tgtcgatccc gtcgaccacc gcggtgccct catcgggcac acgcaggccc tcaatcatct
      721 ccagcgtggt ggtctttcct gctccgttcg gaccgaggat gccgaaaatc tccccacgat
      781 tgacctggaa gctgacgtga tcaaccgccg tgatggcgcc gtagcgcttg accaaatcct
      841 gaacatcgac gatgacctgt tccatcggca acttgtcatt gcgtgtgcag caaagcaatc
      901 tcctgccgat acaacaggaa tgcctccgca cgagcacaaa ggattctaac gccgcaatga
      961 ccttcgagat ccgccgggcc acggctcggg atatcaaagc gatcttgccg gtgtgggggg
     1021 aactggcagg cttccacgcg tcgctggatc cggcatttac gccctccccg gagtggcccc
     1081 gcgagtacgg cgcgtacctg aagaccctaa tggcgcgtga ggacgcgctg gcgctcgtcg
     1141 ccaaagagga cctcgaggtc atcgggtacg ccgtcggcag aatcacaacc ctgccgccgt
     1201 ttttcgagca tcggtctcgt ggctacatcc acgatgtgta cgtcagagaa gagttccggc
     1261 ggcgcgggct ggggcggcgc cttgtcgagg agatcctcgt gtggttcgac cgccgcggcg
     1321 taaccctggt ggagctcacg gtcgcggcca acaacgccga ggccatcccc ttctggaagc
     1381 gtttggggtt tttcgtctac atgcatcaaa tgaaaaaaga aattcgggag cagcggatgg
     1441 ggaacggtga acgaagatcc aggtcgaggt gacagatgcg gatcgataag gtacaggctg
     1501 ctgcgcagaa taccgggctc gacggattca tcatcagcac gccggcgaac acgtactacg
     1561 cgtccggctt ccgcgccatc acgtattcgc ggccggtgct ggtcctcgtg tccgacaggc
     1621 cggcgctgat catccccgag ctcgaggaaa cgcacgcgcg tcagcggtcg gccgtcaagg
     1681 acatcaggac gtattcggac atggccctgg ggggactcgg cgggaagtcc acgctgcaac
     1741 tggcgatcga tctcctcgtc gaagtgctcc acgagcgcga gctcaaaggg cggcggcttg
     1801 gattcgagcc cacgggattt tcgcttgaag gttacaacta tctctcagga gcgtggaaag
     1861 cccgcctcga gccgaccaag ggcatcgttg agcgtctccg gatggtcaaa gatgacgacg
     1921 agctgcgatt aatccgcatc ggcgccgaac tcgcggacat tgggatgcag gtcgagtcgg
     1981 acgccagcgt acccggcgcg actgagatcg agatcctcgc gaaagggaac gccgcgatgt
     2041 tgatgcaggc cagcaagcgg tatcccgact tgccgatcac cgccggctcg cggcctgtct
     2101 cgggcgcgaa gaccgtgctc ccccactcga tcccgtcagg aaagccactg gcccgcggcg
     2161 acgtcgtcat ccacgggacg ggctgcatcg tcgacggata ctattcggaa gacgaacgca
     2221 cgattttcgt cggagatcct accgtggagc agcgcaagtt gttcgacgtc atgctcaacg
     2281 cccagcagtc ggccatcgat gcgatccggc cgggagttcc ctgcaagaat gtcgatcgcg
     2341 ccgcgcggca ggtgatcgag gacgcgggct atggaaagac gttcatccac cgcaccggcc
     2401 acggcatcgg catcgacatc cacgagctgc cgttctttgc cgcgagcgat gacacagtgc
     2461 tggaaccggg gatggtgatg tctgttgagc cggggattta tgtggaaggg aagggcggat
     2521 tccggcattc ggacacaatc atcgtgaccg aagacggctg cgacgtcctg acgaagcacc
     2581 cgaagacgct gccagaactg atttttacat gacccgcact aaatgatcgg ccgcgaccgc
     2641 gtgctctccg cgatcgcggg gagatcgacc gaccgggttc cggttgcgtt ctggcgccat
     2701 ttcccgggtg aagatcagcg cgcggagtcc ctcgcggccg cacacgtcgc cttccagaag
     2761 aagttcgatt gggacttcct caaagtcact ccggcctcgg gctactacgg cgacgattgg
     2821 gggttgcggg cgagctacaa gcctaacccg gccggcacga gacaatacac cgatcgtcca
     2881 atcaagaagc cgggtgattg gtcgcacctg aaacctcttg acgtaaccgc gggagtctac
     2941 gggcgcgagc ttcacgcgct ccggctggtt cgcgatgctc tcccgcaact gctcgtcct
//
LOCUS       DBNGSB010000113         5078 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_996979, whole genome shotgun sequence.
ACCESSION   DBNGSB010000113 DBNGSB010000000
VERSION     DBNGSB010000113.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 5078)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5078)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5078
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_996979"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..181)
                     /locus_tag="AC159B_03070"
     tRNA            complement(105..181)
                     /locus_tag="AC159B_03070"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:His,seq:gtg)
     gene            351..530
                     /locus_tag="AC159B_03075"
     CDS             351..530
                     /locus_tag="AC159B_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376765.1"
                     /translation="MHDSTILAVPSRARKGRWWVRVTTASMILSVAMLVVGWVVSMTL
                     LVTSSRLLEAVFALP"
     gene            complement(541..1035)
                     /locus_tag="AC159B_03080"
     CDS             complement(541..1035)
                     /locus_tag="AC159B_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376766.1"
                     /translation="MRFVVGMLALSLLAISPVVFAQGGGYGSGGGGGGDYDYGSPMSG
                     GVVPQLRTARTHAMNAAGSEALGGAIDHLAHVVNCIEGSRGANYNSQTANPCQGQGNG
                     LIPDLQSDSSRGRMEATKALEFARQADRLAVETLKVTDLAKVKTAAKRVADLLGEALT
                     ATGQ"
     gene            complement(1093..3459)
                     /locus_tag="AC159B_03085"
     CDS             complement(1093..3459)
                     /locus_tag="AC159B_03085"
                     /inference="COORDINATES: protein motif:HMM:NF017262.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA];
                     GO_process: GO:0046688 - response to copper ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC/CopD family protein"
                     /protein_id="HIR3376767.1"
                     /translation="MRRRILPPLLLALFTLFLPSELSAHGILSRTIPQAGTTVKSAPK
                     SVTLVFSEPVDGVYSGATVVDAAGRTMSGRAVISSDGRTMTVPLNGGAGILTVRWRVL
                     SLLDGHTTTGRFRFAIRAAPPATKTPDAPPADVPLPLSAEETQAASEGETDVARVLVR
                     WIALAAVLLTAGTAFFQWLVIRPDPSLPEVEGTLRPIAVGSAIVMAVSAMAEFLLQAS
                     ALLDLPFSGVLTSGALTGMIVDTKAGWSALGRIILAAILLVPATPRGRVFRLASVIWF
                     AVVAIVFALLSNPGVVAGSRHFAHLVTLLLVATVYGLMTAVGAVILPLVPDLKLPEGA
                     WAPPVASLMAVGAITISSHAAGQGIVAATIDWCHIIAAAAWIGGLLPLAVVLARTSPQ
                     DRGDLARRIVPRFSQVAGWSLLILVVTGIYSSWLHIPALRAFIETLYGRALLTKLLFV
                     VPAIILGAVNRFVFRPRIAGGANGTNRRNFLLSVRAEVLLAGGIILAVAVLTITPPAS
                     VSYSSTTRPPVRLAGFAGNQRVLLTVSQERDSNRFTVEVTGMRSEADVQIALTELRAQ
                     RNAVVLPLASAGQGRFLGDGPRIDESGMWEVEVRIRSRTAPAVSVRFPLRTGAPARER
                     DQSALQLLERAKSTLAAARTWSTVEQTTDGTGNGIVTVFEAAQPDRLRYRTSSNVEGI
                     IIGVRRYQRQAGGGWEQDTLPSPPAISGPFVPYLGQIGAAIMGRSERCGSEACQVVLW
                     ETVEPGASFAGWIGLDTLQIHRLLMVAPSHFMTAELRGVNLPITITPP"
     gene            complement(3456..3932)
                     /locus_tag="AC159B_03090"
     CDS             complement(3456..3932)
                     /locus_tag="AC159B_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376768.1"
                     /translation="MRRNTTNKIFWIGLGVIGLGVFIAIILMAGSSAPPRRGDHWHAR
                     YLIVICGVPRPQFPFTRSGVHTHGDGVIHIHPENRSEVGRNANLGRFFASAGAVIARD
                     RIELPDGTTYRNGDRCPDGSTGMLRLMVNGRPSDAFERYTPRDGDTVVVEFGPPRQ"
     gene            3996..4970
                     /locus_tag="AC159B_03095"
     CDS             3996..4970
                     /locus_tag="AC159B_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012321.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3376769.1"
                     /translation="MRITFLWPTLLWGLLLIPLLAVLYVRVLRRPARHPVTFSTQRTL
                     AHAAGASQRYRRHFGAALFGVAAMLLVLSAARPVAPIPVPADRAAIILAIDVSGSMRS
                     QDILPTRLEAAKSAATTFIDSVPDRVRIGLVAFAGFATVLVPPVTEHQRIKDAIVGLG
                     YARRTAIGEGLLESVAALPGRVRPGPDGALPLAPPGAKPSGVVVLLSDGNSNYGIAPL
                     DAAEIARRQDVTVYTVGIGQPMSTGAWQIGGSLDQETLQSIATITGGTYYHASSAEGL
                     RHIYRRLARSIGWERRPTEISAVTAAGAAALFFTSLSLSWFLTHPAGL"
BASE COUNT          983 a         1640 c         1520 g          935 t
ORIGIN      
        1 ggtggtgggg gtggcacatc gctcattggc cgcaccatcc gatagcaatc cggtagcaaa
       61 atcggcctcc cgaggagacc gatctgccaa aaccctctat tttctggggt gaccgacggg
      121 acttgaaccc gcggcctcct gggccacagc caggcgctct accaagctga gctacggcca
      181 ccgcttcgcg aaaattctaa catacttgcg ctaaacgagt gttccccctc accctaccct
      241 ctccccgtcg gggagaggga aatgcggttg gccctccggc gccaaacttg ctggggatcc
      301 aggagtatga tgcgctgtct gggggcactt ccttgatgga ggtgttaccg atgcacgaca
      361 gcacgatcct cgccgtcccg tcccgcgcac gaaagggtcg ctggtgggtt cgagtgacga
      421 ctgccagtat gattctcagc gtcgccatgc ttgtcgtcgg ttgggtggta agcatgacgc
      481 tgctggtgac gagcagccgc ctgcttgagg cggtcttcgc cctaccctaa gaactacagt
      541 ttactgtccg gtcgccgtta gggcttctcc caatagatcg gcgacgcgct tcgccgctgt
      601 ctttaccttc gccagatctg tgaccttgag tgtctcaacg gcaagccgat cggcttgtcg
      661 ggcaaactcc agcgctttgg tcgcttccat gcgcccgcgg ctgctgtccg attgaaggtc
      721 agggatcaga ccattcccct gtccctggca cggattggcc gtctgcgagt tatagttcgc
      781 accacgagag ccttctatac aattgacgac atgggccagg tggtcaatcg ctcccccaag
      841 ggcctctgag ccggccgcat tcatggcatg cgttctcgcg gtccgtaact gcggaacgac
      901 tcctccactc ataggagacc cgtaatcgta atcgccgccg cctccgcctc cgcttccata
      961 tcctccaccc tgcgcgaaga cgaccgggga tatcgcaagc agcgacaacg ccagcattcc
     1021 aacgacgaat ctcatgtgca cttcacccct tccctaccct gcgaccaaac cgtaacggac
     1081 acatcacgag ccctacggcg gagtgatcgt tatcggtaaa ttgacccctc gaagctccgc
     1141 cgtcatgaag tgggacggcg cgaccatgag caaccggtgg atctgcagtg tgtcgagccc
     1201 aatccatcca gcgaaggatg cccccggttc gaccgtttcc cacagcacaa cttgacacgc
     1261 ttctgatcca caccgttccg aacgtcccat gatcgccgcc cctatctgtc caagatacgg
     1321 aacgaacgga ccggaaatgg ccggcggcga gggcagcgta tcctgttccc atccgccgcc
     1381 tgcctgtcgc tgataccggc gtacaccgat gataatgccc tcaacattcg acgaggtgcg
     1441 gtagcgaagc cggtccggct gtgctgcctc gaatacagtg acgatcccgt tgccggttcc
     1501 gtcggtggtt tgctcaaccg tactccaggt ccgggccgcg gccagcgttg actttgcccg
     1561 ttccaacaat tgaagcgcgg attgatcacg ttcgcgtgcc ggagcaccgg tcctcagcgg
     1621 aaacctgacc gagacggcgg gtgcagttct cgatcgaatg cgcacttcaa cctcccacat
     1681 cccgctctca tcgatccgag gaccatctcc cagaaaccgc ccctgccccg ccgaagcgag
     1741 cggcaagact accgcattcc gctgcgcgcg caattctgtg agggcgattt gaacatcagc
     1801 ttcggatcgc atgcctgtga cttcaacggt gaatcgattc gagtctcgct cttgcgacac
     1861 tgtgagcagg acacgttggt ttcctgcaaa gcccgcgaga cgcaccggag ggcgcgtcgt
     1921 ggaagagtac gacactgagg cgggcggcgt gatggtcagc acggccaccg cgagaataat
     1981 gccgccggcc agcaggacct cggctcgaac gctgagcagg aagttccggc ggtttgtgcc
     2041 gttggcgcct ccggcgatgc gcggccggaa cacgaagcgg ttaaccgcgc cgaggatgat
     2101 cgccggaacg acgaacagga gcttcgtgag cagcgcgcgg ccatacagcg tctcgatgaa
     2161 cgcgcgcagc gccggaatgt gtagccacga gctgtagatc ccggtgacga cgaggatgag
     2221 gagactccat ccggctacct gcgaaaatcg aggtacaatg cggcgcgcga gatcgccgcg
     2281 atcttgtgga gatgtgcggg ccagcacaac ggccaggggc aagagaccgc cgatccacgc
     2341 cgcggccgcg atgatgtggc accagtcgat ggtggctgct acgatccctt gccccgcggc
     2401 gtgcgagctg atagtaatcg cgcccaccgc catgaggctg gcgacgggag gtgcccatgc
     2461 cccttccggg agtttgaggt ccgggaccaa cggcaagatg acagccccca ccgccgtcat
     2521 cagcccgtag accgtcgcca cgaggagcag tgtcacaagg tgcgcaaagt ggcggctgcc
     2581 ggcgacgaca ccgggattag aaagcagagc gaacacaatc gccaccacgg cgaaccagat
     2641 gacggaagcc aacctgaaca cacgtccgcg aggagtggcc ggcacaagta agattgctgc
     2701 aagaatgatt ctcccaagcg cgctccagcc ggccttggta tccacgatca ttcccgtaag
     2761 cgcgccgctc gtgaggaccc ctgagaacgg gagatcgagg agcgccgacg cttgaagcag
     2821 aaactcggcc atcgcggaaa cggccatgac gatagcactc ccaacagcga tcggacggag
     2881 tgttccttcg acctccggaa gcgagggatc cggccggatg accaaccatt ggaagaatgc
     2941 tgtgccggcg gtaagaagga cggcggcgag cgcgatccaa cggaccagaa cgcgcgcgac
     3001 gtctgtttca ccctcacttg cggcttgagt ttcctcggcg gacaaaggca gtggcacgtc
     3061 cgcgggcggc gcgtctggag ttttcgttgc gggcggcgct gctcgaatcg caaaacggaa
     3121 tcgacccgtg gtggtatgac cgtccagcag cgagagcacc cgccaccgca cggtgagaat
     3181 tccggcgccc ccgttcagcg gtacggtcat ggtacgccca tcggaagaaa tgaccgcgcg
     3241 gccgctcatt gttcgcccag ccgcatcgac caccgtggcg ccgctataga ctccatcgac
     3301 aggctcgctg aagacgagcg tgaccgactt cggagcgctc ttcacggtcg ttccggcctg
     3361 gggaatggta cgtgacagaa tcccgtgcgc ggagagttcc gatggaagga aaagtgtgaa
     3421 cagtgccagg agaagcgggg gcagaatcct tcgtctcatt gcctaggcgg accaaactcg
     3481 acgactaccg tgtcgccgtc acgcggagta tagcgctcga aagcatcaga aggcctccca
     3541 ttgaccatga gacgcagcat tccggtgctt ccgtccgggc agcgatctcc gtttcgatat
     3601 gtcgtgccat cgggaagctc aatacgatcc cgcgcgatca cggcgccggc gctcgcgaag
     3661 aagcgcccga gattcgcgtt ccttcccact tcgcttcggt tctcagggtg gatgtggatc
     3721 actccgtcgc catgtgtgtg aactccactc cgggtgaaag ggaattgcgg ccgaggaacg
     3781 ccgcagatga cgatcagata ccgcgcatgc cagtgatcgc cccggcgtgg gggcgccgag
     3841 gagcccgcca ttaagataat cgcaatgaac acgcccaggc cgatgacacc cagcccaatc
     3901 caaaagattt tgttggttgt gtttcgcctc atgccggatt ctcccgggtt gaggcgcagg
     3961 caggcggtgc agtcccggcg aacgctcccc gcgatatgag gatcacgttt ctttggccga
     4021 ccctgctttg gggactgctg ctcattcctc tccttgccgt gctgtacgtt cgggttctac
     4081 gccgacccgc gcgtcatcct gtgacgttct ctactcaaag aacccttgcg catgcggctg
     4141 gagcctcgca acgctatcgc cgtcacttcg gagctgcgct gtttggggtt gccgcgatgt
     4201 tactggtgct gtcagcggcc cgccccgtgg ccccgattcc ggtgccggcc gatcgagcgg
     4261 cgatcatcct cgccatcgac gtcagcggca gcatgcgctc ccaagacatc ctgccgactc
     4321 gcctcgaagc ggcgaagtct gccgcgacta cctttatcga ttcggttcct gaccgggttc
     4381 ggattggact cgtcgcgttc gcgggtttcg ccacggtcct cgtaccaccg gtcaccgagc
     4441 atcaacgcat caaggatgcc attgtcggat tggggtacgc gcgccgcacc gcaatcggcg
     4501 agggactcct ggaatcggtc gcggcactac cgggacgcgt ccggcccgga ccggatggtg
     4561 cacttccgct ggcgcctccc ggcgccaagc cctctggggt cgttgtgctc ctctccgacg
     4621 gcaacagcaa ctatggcatc gcgcccctgg acgccgcaga aatcgcccga cggcaggacg
     4681 tgaccgtcta tacggttggc atcggccagc ccatgtcgac gggcgcgtgg caaatcggag
     4741 gctcactcga tcaagagacg ctgcagtcga ttgcgacgat cacaggcggg acgtattatc
     4801 acgcatcatc cgcggagggc ctgcgccaca tctaccgccg gctcgcgcgc agcattggat
     4861 gggaacggcg acccactgaa atctccgcgg tgactgcggc gggagccgcc gctttgttct
     4921 tcacttccct ctcgctctca tggttcctga cacaccccgc cggtttgtag atatcttaac
     4981 gcgtcctaat cacgacgagt ttatcgacgc cggattccgc cccccagact tccccggggc
     5041 gtccaaggat ctggcgcaca ttcgccggcg aactcgga
//
LOCUS       DBNGSB010000114        10071 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2992526, whole genome shotgun sequence.
ACCESSION   DBNGSB010000114 DBNGSB010000000
VERSION     DBNGSB010000114.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 10071)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10071)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10071
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2992526"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="AC159B_03100"
     CDS             complement(<1..611)
                     /locus_tag="AC159B_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HIR3376770.1"
                     /translation="MSIKGAIIKIAGPAVIAKGMTGARMYDVVRVGNEGLLGEIIRLD
                     GETAFVQVYEDTSGLKVGEEVESTGNPLTVELGPGLLLGVFDGILRPLESVRREKGDF
                     ISRGATAVALDRTKKWSFTPTVKAGDQVNPGDALGWVQEFGFKHQVLGPPDVKGGEVS
                     EVKQGELTVTETVCRLKDGTELQLMHRWPVRQPRPVKQRLEPR"
     gene            complement(720..1037)
                     /locus_tag="AC159B_03105"
     CDS             complement(720..1037)
                     /locus_tag="AC159B_03105"
                     /inference="COORDINATES: protein motif:HMM:NF014092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HIR3376771.1"
                     /translation="MYKVAVITDSETATGFRLAGVEVREVSSPQQALEQLRSLLTQDY
                     GVVAVNEDLMKGLDEGIRRLMRGRETPILVPFPAPRVQLESGEEYLARLVKEHIGVFV
                     KLR"
     gene            complement(1030..2061)
                     /locus_tag="AC159B_03110"
     CDS             complement(1030..2061)
                     /locus_tag="AC159B_03110"
                     /inference="COORDINATES: protein motif:HMM:NF014094.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HIR3376772.1"
                     /translation="MPDFPYINARVRAMRSRLLDPGRVEELLGLPSLEAFIQALGSTP
                     YAHEIQEAQSRTTDPLRIVDDALARNFYLTTTKILSFAESKARQLIELVLMRWDLSNI
                     RSILRGKHAGRRDEEILAGLIPAGRLNEPALKELVAQPDIAGVAGALGGMDHPLAVAL
                     VEGLREYLETKDLLVLELQLDRYYAAYGLRIASGRGHNEEVVRRFLQDEVDATNVRTA
                     VKLQRVNGLRKDEKLRFFIPGSRLSENTFLAMADPQTVERGVQAARLQGFQIKSVHDD
                     PGALERELESTMLRAQANLYLQDPLGIEIVIGYLAMKYSEVVNLRLIGRSTALGIPRD
                     RIRKEMALV"
     gene            complement(2082..2672)
                     /locus_tag="AC159B_03115"
     CDS             complement(2082..2672)
                     /locus_tag="AC159B_03115"
                     /inference="COORDINATES: protein motif:HMM:NF014093.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HIR3376773.1"
                     /translation="MESELVAILQKEADAEREGLLDGAKAQAEQIIAEAQKEADAQLA
                     ARRQELEAERQSGRVRAESAEQVRAVALVLQAKDGVLADVFAKAEAELAGMAQDRTRY
                     AALLRGLIREAAAGLDGRLVVEVHPSDLDVARTAVRELKLDAEVKPADEIRGGARLST
                     ENGRFVVENTLASRIERVKPVIAPEVAALLWGGRGS"
     gene            complement(2676..2990)
                     /locus_tag="AC159B_03120"
     CDS             complement(2676..2990)
                     /locus_tag="AC159B_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HIR3376774.1"
                     /translation="MKGALVMMTVLTLTLIVAGVAWAEEAQAGQPAGASAGMLGLAAA
                     IAVGLGAIGTAWAQSRIGAAAAGAIAERPEMGGLMLVFLALPETMVILGFLVAFFLIG
                     NI"
     gene            complement(3051..5057)
                     /locus_tag="AC159B_03125"
     CDS             complement(3051..5057)
                     /locus_tag="AC159B_03125"
                     /inference="COORDINATES: protein motif:HMM:NF013647.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HIR3376775.1"
                     /translation="MIVEMSRVTVLGPKRLLGQVIDEVQRLGSLHVERIESEELAQVS
                     RAQLTPEESQALQILERSIGRVEGILTLLPPGPSEQMVTGEMPDSTSTEALDAWVADL
                     EPRVRDLTRRRLELEEERTLIASFEGAVRVLSPLLSALAGSRSFETIGFILNTKDLTP
                     VAALRNELASVTENRVEVVSRTVDENRIGVVVAFRKADSDRVRSVLARAGAVELRLPG
                     RFAQGNAADTIALMERRKAEIPREIEQVDAELLDINQRERPRLMAVRASMADTLAQKK
                     IVGDMAQSHYAFVLHGWAPTRTLDEIRARLGARFGKDVVVYDQPADPHHYPERVPVLL
                     DNHPLIRPFQRLLALFLPPRYGAWDPSPVLAITFPIFVGLVIGDVAYGMLLFWLGWKF
                     RTMARAGKALTINFLNVRFPPDLLADLSFLIRISAFWMIVFGVIYLEFFGNLPELLAH
                     KYHLPIHPIYNRVAEAQTSYFQLIIAAGIVMIFFGLIIHFVQALRHRHFVGVFEAIVI
                     TLGTAGLFLFLGASGDKLPSVLGPIGLYLFLGAVAVAVISLVVDRDVMKRLLWLLEST
                     SAFGHILSHARLLAFGLAAAALATAANEVGPAMSQQFGVPGFVGRIFGWILAAVFQTM
                     FFVFTIMGHVIQPARLHWVEFFTKFKYHEETGRAYRPFQKTSAP"
     gene            complement(5070..5417)
                     /locus_tag="AC159B_03130"
     CDS             complement(5070..5417)
                     /locus_tag="AC159B_03130"
                     /inference="COORDINATES: protein motif:HMM:NF028309.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit subunit G family protein"
                     /protein_id="HIR3376776.1"
                     /translation="MTAKHGPSDENPLQLIAHKEKELEERLAQTREEARRLIEDARRR
                     AEQIREEARREGEALATRVRAELTGEAAAISKERLARAQAEVSRIQSLANERVPDAVK
                     LVIERVLGDLSDR"
     gene            complement(5498..6928)
                     /locus_tag="AC159B_03135"
     CDS             complement(5498..6928)
                     /locus_tag="AC159B_03135"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HIR3376777.1"
                     /translation="MNPADLWSIVPELWLTGLAIFVLLVDLFTARKTVVGVVAVIGLL
                     LSIIPTGVLLGQDPRTVLAGSYAVDGFAVFFKILTVIATALVILAAMDWLPGQTQFEG
                     EFYALLVFTAVALILMAASTDIILLALSVEFVSLTSYVLAGYLKGNLKSNEAGLKYFL
                     FGAAASAVMFFGFSILYGLTGTTNLYEIARRVTGAPIHALSLSVALMLAGFGFKISMV
                     PFHQWTPDVYEGAPTPVAAYLSVASKAAGFAALVRVLMVVVDPRQLNWVLLLAILAAV
                     SMTLGNLLALPQRNIKRMLAYSSISHAGFLLIGVVAFRGDFGTPGLLIYLLGYTFTQL
                     GAFFVAALIGRQLGTDDIPDYAGLSRRAPVSALLMAVFMLSLTGLPPTSVFVGKFYVL
                     AGAIDNGLLWLAIVGVVNSIISLYYYAGVIRAMYIMPAPSTVPMDEPAGLQVALGITG
                     LGTLLIGLYPQPIIDLARSAVLLLRI"
     gene            complement(6932..8434)
                     /locus_tag="AC159B_03140"
     CDS             complement(6932..8434)
                     /locus_tag="AC159B_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048039 - ubiquinone binding [Evidence
                     IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 4 family protein"
                     /protein_id="HIR3376778.1"
                     /translation="MSGILSLIIFLPLLGGLIILFIPKSAERVMKQIALIVSLASLAL
                     SLVLVAQFSRGGGMQFEERYSWIPSIGVNYHLAVDGLSLPLLVLTAVLSVLSVIYSWR
                     MDVRLKEYLFLFLLLQTGMLGVFAALDFFLFYVFWEITLVPMYFLIGIWGGPRREYAA
                     IKFFLFTLVGSLAMLLAILIMYFNAAPHTFSMLELIRQQPLADAPLLAALAFWGFFLS
                     FAIKVPMWPFHTWLPDAHVEAPTAGSVILAGVLLKMGTYGFLRVSLPMLPETFKTYAF
                     FIAVLALLGIVYGALVAMAQTDLKKLVAYSSVNHMGYVMLGTAAAAGAIGDPSKAAAA
                     ATALNGAVFEMIAHGVITGALFFIVGIIYDYRAHTRGVDEFGGLGAKLPVYTGLTTMA
                     MLASLGLPGLMGFVAEFLVFLGSFGVFPYLTAVAVTGVIFTAAMFLWTIQRIFLGAPN
                     PRWASLPDMDTREKLTLVPLAVLMVAFGLFPKPLLDVINAAMTQLVTALR"
     gene            complement(8431..>10071)
                     /gene="nuoL"
                     /locus_tag="AC159B_03145"
     CDS             complement(8431..>10071)
                     /gene="nuoL"
                     /locus_tag="AC159B_03145"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HIR3376779.1"
                     /translation="LIFIYSMGYMQGDPRYPRFFAYMSLFASSMLLLVLANNLLTLYA
                     GWELVGLCSYLLIGFWFERPAAARASMKAFVTTRVGDFFMFLGILLIFLHLGTLNFDG
                     IFAGVETGRLTGGLLTLAALLVFGGAVGKSAQVPLHVWLPDAMEGPTPVSALIHAATM
                     VAAGVYLVARTYPLFFGTPGHEALTVVAYIGGITAIMAATIGVVQDDIKRVMAYSTIS
                     QLGYMMMGLGVLGFTAGVFHLMTHAFFKALLFLAAGSVIHAMHTNDIKQMGGLGKIMP
                     ITYVTMVIGGLALAGIPPFAGFWSKDEILLEAYHHDQVLFALGAAGAFLTAFYVFRMI
                     IYTFTGSLRSHTAHPHESPRVMTVPLMILAFFSTVIGLTGAPWWDNPFHHFVRFEGAE
                     GAAFDLAFALRSTAIAGLGVLSAFAVYRWRLVSAASLRRAFGPLYTAVVHKYYWDELY
                     QFAVIRPVIWAARQLRTFDVYIIDGAVNAIGVVFVWFARLYQVFDLYVVDGLVNLVAW
                     TAKAVGGVLRYGQTGRPQNYLLVIALGVIALVVVGVIR"
BASE COUNT         2134 a         3209 c         2987 g         1741 t
ORIGIN      
        1 tcgcgcggtt ccagacgctg cttgaccggc ctgggctggc gcaccggcca gcggtgcatc
       61 aactgcagct ccgtgccgtc cttcagacgg cagaccgtct cggtgacagt caactccccc
      121 tgcttaacct ccgagacctc gccgcctttg acgtcaggtg gaccgaggac ctggtgcttg
      181 aagccgaact cctggaccca accaagagcg tcgcccgggt ttacctgatc ccccgccttg
      241 accgtcggcg tgaacgacca tttcttcgtt cggtcgagcg ctactgccgt cgcaccgcgt
      301 gagatgaaat cgcccttctc tcggcgcaca ctctccagcg gccgcagaat gccgtcgaat
      361 acgcccagga ggagccccgg tccgagctcg acggtgagag ggtttccggt gctttcgact
      421 tcctcgccca ccttcaggcc ggaggtatcc tcgtagacct gaacaaacgc cgtctcacca
      481 tccaggcgga tgatttcgcc cagcaggcct tcgttgccga cgcgcacgac atcgtacatc
      541 cgcgcaccgg tcatgccctt cgcgatgacg gcgggtccgg cgattttgat gatggcgccc
      601 tttatcgaca tgactacctc ctcaaacctc agctggagag ctgaaaaggt gaagagctag
      661 agagcagctg aattgcggtg gcactccagc tttccagctc ttcagctttc cagcttccat
      721 cagcgtaatt tcacaaaaac tccgatgtgc tctttcacaa gcctcgcaag atactcctcg
      781 ccgctctcca gctgtacccg tggcgcggga aatgggacga gaatcggcgt ttcgcggcct
      841 cgcatcagcc gcctaatgcc ctcgtcgagt cccttcatca gatcttcgtt tacggcgaca
      901 actccatagt cttgtgtcag cagggaccgc agttgctcga gtgcctgctg cggagatgag
      961 acctcccgta cctccacgcc ggcaagccgg aaccccgtgg ccgtctccga atctgtgatc
     1021 acggcgacct tatacaagcg ccatctcttt tcttatccgg tcacggggaa tgccgagcgc
     1081 cgtgctcctc ccgatcaacc tgagattcac cacctcgctg tacttcatcg cgaggtagcc
     1141 gatgacgatc tcaatgccga gtggatcctg gaggtagaga ttcgcttgag cgcgcagcat
     1201 cgtcgattcc agctctcgct ctaacgctcc tggatcatca tgaacggatt tgatctggaa
     1261 tccctgcagg cgggcggcct gcacaccccg ctctaccgtc tgcggatccg ccatcgcaag
     1321 aaaagtgttc tcgctgaggc ggctcccagg gataaagaat cgaagtttct cgtctttccg
     1381 aagtccattc acacgctgga gcttgaccgc cgtcctcacg tttgtggcat caacctcgtc
     1441 ttgcagaaac ctgcgaacga cttcctcgtt gtgtccgcgc cccgacgcaa tgcggaggcc
     1501 gtaggccgca tagtagcggt cgagctgcaa ttccagcaca agcagatcct ttgtctcgag
     1561 gtattctcga agaccctcca ccagggcgac ggcgagtggg tgatccatgc ctcccagagc
     1621 acccgcgacg cccgcgatgt ccggttgtgc gacgagctcc ttgagcgcgg gctcattgag
     1681 tcgaccagcg gggatcaaac cggcgaggat ttcctcatcg cgccggcccg cgtgcttccc
     1741 gcggaggatg ctgcggatat tggacagatc ccaccgcatc agcacgagct cgatgagctg
     1801 cctggcttta ctctctgcga agctcaggat cttggtcgtc gtcaggtaga aattgcgtgc
     1861 cagcgcgtcg tccacgatcc gcaagggatc cgtcgtgcgg ctttgcgctt cctggatttc
     1921 gtgcgcgtag ggcgtactgc cgagcgcttg gatgaatgcc tcgagcgacg gcagcccaag
     1981 caactcctcg actcgccccg gatcgagaag ccggctgcgc atcgcacgca ccctggcgtt
     2041 aatatacgga aagtccggca tttccctcca cgacctatga cctacgaccc acgaccgccc
     2101 cacaggaggg cggcgacttc cggcgcaatg accggcttca cgcgctcaat tctcgaggcg
     2161 agcgtattct caaccacaaa gcgcccgttc tcagtcgata ggcgcgcgcc tccgcggatc
     2221 tcgtcggcag gcttgacctc cgcgtcgagt ttcagttccc gcaccgcggt ccgcgcgacg
     2281 tcgagatcgc tgggatggac ttccacgacg agccggccgt ccagaccggc cgccgcctcg
     2341 cggatgagac cgcggagcag ggccgcgtag cgtgttcgat cctgtgccat tcccgcgagt
     2401 tcggcttccg ccttagcgaa cacgtcggcc agcaccccgt ctttcgcctg tagaacgagc
     2461 gccacggcgc gtacctgctc ggcgctctct gcgcgcacgc ggccgctctg ccgttcggcc
     2521 tccagttctt gtcggcgcgc cgcgagctgc gcgtcggctt ctttctgcgc ttcggcgatg
     2581 atctgctcag cctgagcctt ggcgccatcc agcaggccct cgcgctccgc gtcagcttcc
     2641 ttttgaagaa tggcgacgag ttctgattcc atctgctaga tattgccgat aaggaagaat
     2701 gccaccaaga agcctaggat gaccatcgtt tccggcagcg cgaggaagac cagcatgaga
     2761 ccgcccatct ccgggcgttc agcaatcgcc ccggccgccg cagccccgat cctggactgt
     2821 gcccacgcgg taccgattgc tcccaggccg acggcgattg cagcagccag tcccagcatt
     2881 cctgcgctgg cgcccgccgg ctgaccggcc tgcgcttcct cggcccacgc cacaccggca
     2941 actatcagcg tgagcgtcag gaccgtcatc atcaccaatg cacccttcaa tcgactcacc
     3001 ccctcgtatt catcgtaccg ggaatcgtgt gaagcccgcg tcaagaactg tcaaggagcc
     3061 gacgtcttct ggaacggccg gtacgcccgg ccggtctctt cgtggtattt gaattttgtg
     3121 aagaactcca cccagtgcag acgtgcgggt tggatcacat gacccatgat cgtgaacaca
     3181 aagaacatgg tctgaaatac tgctgcaagg atccatccga agatccgacc gacaaaaccc
     3241 ggtacgccga actgctgaga catggccggg cccacttcat tcgccgccgt ggcgagtgcg
     3301 gctgccgcaa gaccgaaggc caggagtcgc gcatgggaga ggatgtgacc gaacgcagaa
     3361 gtgctttcca gcaaccacag gagacgcttc attacgtcgc gatcaactac gagcgagatc
     3421 acggcgaccg cgaccgcccc caagaacaag tacaggccga tcgggccgag cacactgggc
     3481 agtttgtccc cgctcgcgcc caggaacagg aataacccgg ctgttcccaa tgtgatcacg
     3541 atggcctcaa agacgccgac gaagtgccgg tgccgcaacg cctgaacgaa gtggatgatc
     3601 aaaccgaaga aaatcatcac aatcccggcc gcgataatca gctggaagta gctggtctgc
     3661 gcttcagcca ctcggttgta tatggggtgg atcggcagat gatatttatg cgcgagcaat
     3721 tccgggaggt tcccaaagaa ctcgagatag atgacgccaa agacgatcat ccagaaggca
     3781 ctgattcgaa tgagaaagga gagatcggcc agcaggtcag gtggaaaccg cacgttcagg
     3841 aaattgatgg tcagggcctt gccggcgcgt gccatcgttc gaaacttcca cccgagccaa
     3901 aacagtaaca tcccgtaggc cacgtcgccg atcaccaggc ccacaaagat cggaaacgta
     3961 atcgccaaca ccggactcgg atcccaagct ccgtatcggg gcggcaggaa gagcgccagc
     4021 aatcgttgga aggggcgaat caggggatgg ttgtccagca acacgggcac ccgttctgga
     4081 tagtgatgcg gatcggcggg ttgatcatag accacaacat ccttgccgaa ccgagcgccc
     4141 aatcgtgctc gaatctcgtc tagtgtgcgg gtgggcgccc atccgtgcaa tacgaacgcg
     4201 tagtgggatt gcgccatgtc gcccacgatc ttcttctggg cgagcgtgtc tgccatcgag
     4261 gcccgaacgg ccatcaaacg agggcgttcc ctctggttga tgtcgagcag ttcagcatca
     4321 acttgttcga tctcacgtgg aatctcggcc ttccggcgct ccatcaacgc aatcgtgtct
     4381 gccgcgttcc cctgagcaaa gcggcctggg agtcgtagct cgacggcgcc cgcccgcgcg
     4441 aggacgctcc ggacacggtc actatctgcc ttccgaaatg cgacgacgac tccgatgcgg
     4501 ttttcgtcca ctgtgcggct gacgacttcc acgcggtttt cggtgacgct ggccagctcg
     4561 ttgcgtagcg ccgcaaccgg ggtcaggtcc ttggtgttca ggatgaaacc aatcgtctca
     4621 aaggagcggc tgccagcgag ggcgctgagg aggggggaca gaacgcggac ggcaccctca
     4681 aaggacgcga tcagcgtgcg ctcttcctcc agttccagcc gcctccgggt gagatctcgc
     4741 acgcgcggct cgagatctgc gacccaggcg tccagtgctt cagtgctcgt tgaatccggc
     4801 atttccccgg tcaccatctg ttccgacggc ccgggaggca gcagcgtcag gataccttcc
     4861 acacgcccga tggaccgctc gagaatctgg agggcctggc tctcttcggg tgtgagttga
     4921 gccctggaca cctgcgcgag ctcctcagac tctatccgct caacgtgtag tgagcccagt
     4981 cgttgcacct catcgatcac ctgaccgaga aggcgctttg ggcccagcac agtgacccga
     5041 ctcatctcga caatcatgcc gaaccgtacc tatctatccg agagatcgcc aaggacccgc
     5101 tcaatgacga gtttcactgc atcagggacg cgctcgttcg ccagcgattg gatccgagaa
     5161 acctcggcct gggcccgtgc taagcgctcc ttggaaatgg cagcggcctc cccggtcaac
     5221 tctgcgcgca cgcgggtggc cagcgcctca ccttcgcgac gagcttcctc tcggatttgc
     5281 tcggcgcggc gccgcgcgtc ctcgataagc cgcctggctt cctctcgggt ctgcgcgagt
     5341 ctttcttcga gttctttttc cttatgggcg atcagctgca gaggattctc gtcggatggg
     5401 ccgtgcttgg ccgtcattgc gtcaccgtac caaggtgtgg ggcagcatca ggggcacgcg
     5461 ccatcataac aaacggcgcc aaattgcgtc aattgggcta tatccgcaac agcagaaccg
     5521 cggaccgggc gagatcgatg atcggttgag gatacagtcc gatgaggagg gttcccagac
     5581 cggtgattcc gagggccacc tgcagtccgg ccggttcatc catcggcacc gtcgagggag
     5641 ccggcatgat gtacatcgcc cggatcacgc ccgcatagta atacagcgag atgatgctgt
     5701 tcacaacccc cacgatcgcc agccacaaca ggccgttgtc gatcgctccc gcgaggacgt
     5761 agaatttccc cacgaagacg gaggtcggcg gcaagcctgt cagggacagc atgaagaccg
     5821 ccatgagcag cgccgagacc ggcgcgcgcc tggacagacc ggcgtagtcg ggaatgtcat
     5881 cggtgcccaa ctgccggccg atgagcgccg ccacaaagaa cgcgccgagt tgcgtaaagg
     5941 tatatccaag gagatagatc aacaggccgg gcgtaccgaa atctccacgg aacgcaacga
     6001 cgccgatgag caagaatccg gcatgcgaga tgctcgagta cgccagcatc cgtttgatgt
     6061 tgcgctgtgg cagcgcaagg agatttccga gcgtcatcga taccgccgcc aggatggcga
     6121 gcagcagcac ccagtttagc tgtcgagggt ccacgacgac catgagcacg cggacgagcg
     6181 cggcgaatcc ggccgccttt gaggccacgc tgagatacgc ggccaccggc gtgggcgctc
     6241 cctcgtagac gtcgggcgtc cactgatgga acgggaccat cgagattttg aagccgaagc
     6301 cggctaacat cagtgccacg gacagcgaca gtgcgtggat gggtgcgccg gtgacgcggc
     6361 gggctatctc gtacagatta gtcgtccccg tcaggccgta gaggatggag aaaccgaaga
     6421 acatcaccgc ggaggctgca gcgccgaaca ggaagtactt gagtcccgct tcgttgcttt
     6481 tcaggttgcc cttcaagtac cccgccagca cgtaggaagt caggctgacg aactcgaccg
     6541 agagcgcaag cagaatgata tcggtgctcg ccgccatgag aatcaacgcg accgcggtga
     6601 acaccagtaa ggcataaaac tcaccctcga actgggtctg gccaggcaac caatccattg
     6661 cggcaaggat gaccagcgcg gtggcgatga cggtgagaat tttgaaaaaa acggcgaacc
     6721 catccaccgc gtaagagccg gcgagcaccg tgcgcggatc ctggcccagc agcacgccgg
     6781 tagggatgat cgacagcaag aggccgatga ccgccacgac tcccacaacc gttttgcggg
     6841 ccgtgaacag atcgacgagc agcacaaaga tggccaggcc cgtgagccag agctccggaa
     6901 cgatcgacca gagatccgca gggttcatga gttaccgaag cgcggtgacg agctgggtca
     6961 tcgccgcatt gatgacatcg agcaaaggct tgggaaacag cccgaacgca accatgagga
     7021 ccgccagcgg tacgagcgtg agtttctccc ttgtgtccat gtctggcagg gacgcccagc
     7081 gcggattcgg cgctccaagg aagatccttt ggatggtcca gaggaacatc gccgccgtaa
     7141 agatgacgcc ggtcacggcc acggccgtca ggtagggaaa cacgccgaac gagccgagga
     7201 agaccaagaa ctctgcgacg aatcccatta agcctgggag cccaagggac gccagcatgg
     7261 ccatcgtcgt gagtccggtg tagaccggca gcttggcccc caacccgcca aattcgtcca
     7321 cccctcgcgt gtgggcgcgg tagtcgtaga tgattcccac gatgaagaac agcgcacccg
     7381 taatgacccc atgcgcgatc atctcgaaga ccgcaccgtt gagcgcggtc gccgcggcgg
     7441 ccgccttgga cgggtcgccg atcgcgcctg ccgccgccgc cgtgccgagc atgacgtacc
     7501 ccatgtgatt gaccgagctg taggccacga gcttcttcag gtcggtctgg gccattgcca
     7561 cgagcgcgcc gtagacgatc ccaagcaacg ccagcacagc gatgaaaaat gcgtacgtct
     7621 tgaacgtttc cggcagcatc ggcaggctga cccgcaggaa cccgtaggtg cccatcttca
     7681 ggaggacgcc ggcgagaatc accgagccgg cggtcggcgc ttcgacgtgc gcgtcaggca
     7741 gccaggtgtg gaacggccac atcggcacct tgatggcgaa gctcaggaaa aacccccaga
     7801 acgcgagggc ggccagcagc ggcgcatccg cgagcggctg ctgcctgatc agttccagca
     7861 tgctgaacgt gtgcggcgcc gcattgaagt acatgatcaa gatcgccagc agcatcgcga
     7921 gcgagccgac cagggtgaag aggaagaatt tgatggccgc gtactcgcgc cgcggtcccc
     7981 cccaaatgcc gatcaagaaa tacatcggga cgagcgtgat ctcccagaac acgtagaaca
     8041 ggaagaagtc cagcgccgcg aacacgccca gcatcccggt ctgcagcaac aggaagagaa
     8101 acaggtattc cttgagccgc acgtccatgc gccatgaata gatgaccgac agcacgctca
     8161 agacggcggt gagcacgagg agcggcaggc tcagcccgtc cacggcgagg tgatagttca
     8221 caccgatgct gggaatccag ctgtagcgct cctcaaactg catcccgccg ccacgactga
     8281 actgcgccac gagcaccagc gacagcgcca gcgacgccag ggagacgatc agcgcgatct
     8341 gcttcatcac tcgctcggcg gacttgggga tgaacagaat gatcagtcca ccgagcagcg
     8401 ggaggaaaat gatcaggctg agaatgccgc tcatcgaata acccccacga caacgagtgc
     8461 gatcacgcca agcgcgatga ccagcaggta attctgcggc cgtcctgtct gcccgtagcg
     8521 cagcacgccg cccaccgctt ttgccgtcca ggccaccaaa ttgaccagcc cgtcgaccac
     8581 atacagatcg aacacctggt acaggcgcgc gaaccagacg aagaccacgc caatcgcgtt
     8641 caccgcgccg tcgatgatgt acacgtcgaa cgtgcgcaac tgccgcgccg cccagatgac
     8701 cgggcggatg acggcgaact ggtagagctc gtcccagtaa tacttatgca cgaccgctgt
     8761 gtacagcggg ccaaacgcac ggcgcagcga cgccgctgac accaagcgcc agcggtagac
     8821 ggcgaacgcg ctcaggacgc ccagtccggc gatggcggtg cttcgcagtg cgaacgcgag
     8881 atcgaacgcc gctccctcgg ccccttcgaa acgcacgaag tgatggaacg ggttatccca
     8941 ccacggtgcg ccggtcaggc caatcaccgt ggagaagaat gccaggatca tcaggggcac
     9001 cgtcatcacc cgcggtgact cgtgcgggtg ggcggtgtgg ctgcgaagcg aacccgtgaa
     9061 cgtatagatg atcatgcgaa agacataaaa ggcggtcagg aacgcgccgg cagctcccag
     9121 cgcgaacagg acctgatcat gatggtaggc ttcgagcagg atctcgtcct tgctccagaa
     9181 gccggcaaac ggcgggatgc cggccagcgc gagaccgccg atcaccatcg tcacgtacgt
     9241 gatcggcatg atcttcccga gcccacccat ctgtttgatg tcgttggtgt gcatcgcgtg
     9301 aatcaccgac cccgccgcga ggaagagcag cgctttgaag aacgcgtgtg tcatcagatg
     9361 aaagacgccg gcggtgaacc cgagaacacc gagccccatc atcatgtagc ccagctggct
     9421 gatcgtagag taggccatca cgcgcttgat gtcgtcctgg accacaccga tcgtcgccgc
     9481 catgatggcc gtgatgccgc cgatataggc cactacggtc agcgcctcgt gcccgggagt
     9541 gccaaagaag agcggatacg tgcgcgcgac aaggtaaacg ccggccgcaa ccatcgtggc
     9601 ggcgtggatg agcgcggaca cgggtgtggg accttccatg gcatccggca gccacacgtg
     9661 gagcggaacc tgggccgatt tccccacggc gccgccgaac acgagcaggg ccgcaagcgt
     9721 caggagcccg ccggtcagac gtcccgtttc tacccctgca aagattccgt cgaagttcag
     9781 cgtcccgagg tggaggaaga tcagcaggat gcccaggaac atgaagaaat ctccgacgcg
     9841 cgtggtcaca aacgccttca tcgacgcccg cgcggcggca gggcgctcaa accagaaccc
     9901 gatcaagagg taggagcaca gacccacgag ttcccagccg gcgtataacg tgagcaggtt
     9961 gtttgccagc acgagcagta gcattgatga cgcgaacagc gacatgtatg cgaagaatcg
    10021 gggatagcgc ggatcgccct gcatataccc catcgagtag atgaagatca g
//
LOCUS       DBNGSB010000115         2473 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_232964, whole genome shotgun sequence.
ACCESSION   DBNGSB010000115 DBNGSB010000000
VERSION     DBNGSB010000115.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_232964"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="AC159B_03150"
     CDS             <1..415
                     /locus_tag="AC159B_03150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3376780.1"
                     /translation="VHGGFLASGLRSPVLDGVFITGDAAGQCLPVTGEGIRTAALAGC
                     ACGDLVQQLLDGSLSQDEAAIAYTRFVARERRRFRRLLWTNMALLILPQPLIGKAARL
                     FSNPGPLRFFMRHYLGIFRQPSLRSTTAAFSDSVS"
     gene            complement(513..601)
                     /locus_tag="AC159B_03155"
     tRNA            complement(513..601)
                     /locus_tag="AC159B_03155"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(565..567),aa:Leu,seq:caa)
     gene            706..>2473
                     /locus_tag="AC159B_03160"
     CDS             706..>2473
                     /locus_tag="AC159B_03160"
                     /inference="COORDINATES: protein motif:HMM:NF014759.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HIR3376781.1"
                     /translation="MSSRKLILIDANGLVYRAFFALPYFTTTDGRPTNAVYGFTTMLL
                     KVMDEEKPDHVAAAFDKAAPTFRHTAYKEYKATRQRMPDDLRPQIKTAKEVLEALKIP
                     IYEVEGYEADDILATIARKAAAEGFEVVIVTGDLDVLQLVDEHTRVMVTSRGISETTV
                     YDVDKFQERFGFEPSRLPDYKGLKGDSTDNIPGVPGIGEKTAGQLIQQFGSVEELLDR
                     LDRVSSKQRDLLQSRSEQILQSKHLTTLVTDVPLEWSWEDLRRRAPDQERLAELFASL
                     EFKSLLERVGVVEEPKPQGRYQQVSRPEELLDGASAANEVGLYLQRGEGHPLDAPLTG
                     VALSALPGEVQFLTIGDDRFPDTLRAIFESAALKVTGDVKADLLTLRRFGIQPHRLAF
                     DVAIASYLLNPGRRTHTMGTAAWEHLGWRLRMEDEEEGLPLDSDPVGEASEAADVLLR
                     LRPVLEARMRERQVYALFTDIEMPLATVLAEMEAAGVAVDAPYLEELSAEMDRRLADL
                     AAEIYRLAGGEFNISSPKQLSFVLFEKLQLPSMKKTKTGHSTDAEVLEFLAPQHEIAA
                     KIVAHRELTKLKTTYVDVLPRLI"
BASE COUNT          516 a          721 c          765 g          471 t
ORIGIN      
        1 ggttcacgga ggcttccttg catcagggct acgctctccg gttctggatg gcgtgttcat
       61 caccggtgat gcggccgggc agtgcctgcc cgtcacaggg gaagggatca gaacggcggc
      121 cttggcaggg tgtgcgtgcg gagatctagt gcagcagttg ctagacggat ctctgagcca
      181 agacgaagcg gcgatcgcct acacgcggtt tgttgcacgc gaacgcaggc gtttccgtcg
      241 actgttatgg acgaacatgg ctctgctgat tcttcctcaa cccttgattg gaaaggcggc
      301 gcgcttattc agtaatcccg gaccgctgag atttttcatg cgtcactacc tcggcatctt
      361 tcgccagcca tctcttcgat ctaccacagc ggcattttcc gattcagttt cctgaggcga
      421 gtctgaattc agaggtggaa catggttggg ctggatgtgc ttcgcacgat cctaacgcgg
      481 caggagaatg acggtggccc gttacgactg ggtggtgccg ggggagggag tcgaaccctc
      541 acgggcccta cggcccacgg gattttgaga cccgcgcgtc agccagttcc gccaccccgg
      601 cggtcgcaaa ccgagtatag aagtcctctc tagcctcgtc aacgcacatc ataggtgcgg
      661 tgttcctcgc catcgaacca caaaaacgag aaggtgtccg ccagaatgtc ctctcggaaa
      721 ctcatcctca tcgacgcgaa cggccttgtt tatcgcgcct tttttgcgct gccctatttc
      781 accacgaccg acggccggcc gacaaacgcc gtgtacggct tcacaaccat gctcctcaag
      841 gtaatggacg aggagaagcc cgaccacgtc gcagcagcgt tcgataaagc ggcgcctacg
      901 ttccgccaca ccgcgtacaa ggagtacaag gcaactcgtc agcggatgcc cgacgacctg
      961 cgcccgcaga tcaaaacggc caaagaggtc ctcgaagcgt taaagattcc catctacgag
     1021 gtcgagggct acgaagccga cgacatcctc gcgacgatcg cacgaaaggc cgcggcagaa
     1081 ggctttgaag tggtaatcgt gaccggcgat ctggacgtgc tgcagctggt tgacgagcac
     1141 actcgggtca tggtgaccag ccgtgggatc tcggagacga cggtttacga cgtcgacaag
     1201 ttccaggagc gattcggctt tgaaccgtcg cgtctacccg actataaggg gctcaagggc
     1261 gacagcaccg acaacatccc cggcgttccg ggcattgggg agaagaccgc cggtcaattg
     1321 atccagcagt tcggcagcgt ggaagaactg cttgaccgat tggatcgggt atcgtccaaa
     1381 cagcgggacc tgctgcagtc gcgctcagaa cagatcctgc agagcaaaca cttgacgacg
     1441 ctggtgacag acgtgccgct cgagtggtca tgggaggacc tgaggcggcg cgcgccagac
     1501 caggaacggc ttgcggaact ctttgcaagc ctggagttca agagtcttct tgaacgggtg
     1561 ggggttgtag aggaacctaa accgcaaggc cggtaccaac aggtctccag accagaagag
     1621 ctcttagacg gggcctctgc cgcgaatgag gtcgggctgt accttcagcg aggtgaaggc
     1681 cacccgctag acgctcccct taccggcgtt gcgctctcag cgttgcccgg tgaggtgcaa
     1741 ttcttgacca tcggggacga tcggttccct gatacgctgc gggccatctt tgaatccgcg
     1801 gcgctcaagg tgaccggtga tgtgaaggcc gacctgctca cgttgaggcg atttgggatt
     1861 cagccgcaca gattggcgtt cgacgtggca atcgcgtcgt atctgctcaa tccagggcgt
     1921 cgcacgcaca ccatggggac cgcggcctgg gagcatctgg gatggcgcct ccggatggaa
     1981 gatgaagagg aagggctgcc gctggacagc gacccggtcg gcgaggccag tgaggcggcg
     2041 gacgtcctgc tgcggctgcg ccccgttctc gaagcacgga tgcgcgagcg gcaggtctat
     2101 gcattgttca ccgacattga gatgccgctg gccacagtgc tggccgaaat ggaagcggcc
     2161 ggcgtggccg tcgacgctcc ttacctcgag gaactctccg cggagatgga tcgacgcctg
     2221 gcagatttgg cggccgaaat ctaccggctg gccgggggag agttcaatat cagctcgccc
     2281 aaacagttga gtttcgtgct cttcgaaaaa ctacagcttc cttcaatgaa gaagacgaag
     2341 accggacact cgaccgatgc cgaagtcctg gagttcctgg cgccgcagca cgagattgcc
     2401 gcgaagatcg tcgcccatcg cgagctgacc aaactcaaga cgacctatgt cgacgtgctt
     2461 cccaggctca tca
//
LOCUS       DBNGSB010000116         5030 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_1920166, whole genome shotgun sequence.
ACCESSION   DBNGSB010000116 DBNGSB010000000
VERSION     DBNGSB010000116.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 5030)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5030)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5030
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_1920166"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="AC159B_03165"
     CDS             complement(<1..412)
                     /locus_tag="AC159B_03165"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR3376782.1"
                     /translation="MCAAALWGISGVLAKMLFNRQIEPWTLLAIRLTGAFVTLLLILR
                     LTRVSLRVPRAQTGRLIVLGLAMTSAQFTYYVTISLTDVSTALFLQYTAPVFVAMHAW
                     LVEKEPLTALKSGAILLAIVGSYLLVTGAGGIRVS"
     gene            complement(459..1328)
                     /locus_tag="AC159B_03170"
     CDS             complement(459..1328)
                     /locus_tag="AC159B_03170"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF022834.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxamate carbamoyltransferase subunit AllH family
                     protein"
                     /protein_id="HIR3376783.1"
                     /translation="MAQSALSARLKAVSVSRHVHDLLQIPHSGTVLATFDRSCYLDLD
                     GHVVALVLPELLDGPLNLLVQATGDFQFYDLPVGETVENSSAGILIGNAVELTVQQAS
                     IWEPQLARWGRSYLSSVRANLNFLKEILLGETPEGSFATYRTDPAGLSVRARSGMNLL
                     EEGLMHRSPVSTGEAAQHLAGLGSGLTPSGDDVLVGALICLSVLPHEPSELRTAIITR
                     VRGRTTRISEAYLMAAARGEAGEAWHRLLRVLPNGDRAAIASATRRVMAFGETSGSDM
                     LAGFTLAVSTLIS"
     gene            complement(1335..1874)
                     /locus_tag="AC159B_03175"
     CDS             complement(1335..1874)
                     /locus_tag="AC159B_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3376784.1"
                     /translation="MTSRRSQTPELIDRKDIRLRVNDRAYTGNVDVRKTLVDFIREDL
                     AMTGTHVGCEHGVCGACTIILGGRAVRSCLMFAVQADGLEITTVEALGTPERLHPIQE
                     AFRDHHGLQCGFCTPGFLMTAVAFLSETPDPSKDQIREALAGNLCRCTGYQGIVDAVE
                     DAAGRMKMQTRKRASGKPR"
     gene            complement(1871..2740)
                     /locus_tag="AC159B_03180"
     CDS             complement(1871..2740)
                     /locus_tag="AC159B_03180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR3376785.1"
                     /translation="MKPAPFKYSAPETVLETLALLREHGQDGKLLAGGQSLVPLLNMR
                     LARPSVIVDLNAVGELSYIKEDNGDVVIGAMTRQRDAELSPLVTRRLPLLAEALGYVG
                     HPQVRNRGTIGGSIAHADPSAELPAVAAALGARMVVRGPKGRRVVESEKFFLALLTTA
                     LGPDELLVEVRFPAHQKMGTAFLEVARRHGDYALAGVAAILSADGDSIKDCRLAFTGV
                     GPGPVRIPEAEAAVTGNRLTSKILSEVRQIVAGRLDPDSDVHASVEYRKHVAGVLAVR
                     ALRLAHQRATGTR"
     gene            complement(2773..>5030)
                     /locus_tag="AC159B_03185"
     CDS             complement(2773..>5030)
                     /locus_tag="AC159B_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HIR3376786.1"
                     /translation="ISKAQALRGVIAVYTNADLSEAIAGPLPKLIPHPSLIHHKTQYA
                     LVPDTVRHVGEAVAFVVAESRYIAEDALDLIDVEYEPLPPVVDLEQAARDGASLVHGD
                     IGTNVCAHYTQRVGDAEAAFASAPRVFTDRFVIDRGTAAPMECRAVVAQWDGKTRQLT
                     VWDSTQAPIPIRNGLARLLGLPQANVRVIAPDVGGGFGPKIMMFYPEEVLVPFAAMQL
                     DRPVKWIEDRRENFVAMNHERVQIHDAAIAVDDQGRILGVKTVFLYDSGAYIPYGIIV
                     PIVAGTTLPGPYKIPNYHCEFKAVFTNKTIVSPYRGAGRPHGVFVMERLLDRVARELG
                     LDRAEVRRRNLIQPHEFPYDVGLIYQDNAPLIYDSGNYPLLLNRALEMIGYDRWPESR
                     EQHRREGRHVGLGIACYVEGSGIGPYEGCRVTVEPSGKVYAATSVGTQGQGHFTSFAQ
                     IIADALTVPIDDVIITTGDSGAFGWGTGTFASRAAVVAGNAVALAAQAVREKTFKVAA
                     TTLEASVNDLELADGKVFVRGVPGKSLTLAEVAAVANPLRGTIPDEWEQPGLEASRYF
                     APPRGTFAAGVHAAILEVDPETGMIKILKYVVVHDCGKVINPMILEGQVRGGVAQGIG
                     GAFYEKLVYDETGQLLTQTFMDYLLPTATDIPEVEVGHIETPSPLNPLGVKGAGEAGV
                     IPVPALFAQAIDEALSEWNVRITEMPLSPNRILEIIQTGSPQPALTPAPSKIPPASPQ
                     LSLCAPPPASR"
BASE COUNT          930 a         1570 c         1563 g          967 t
ORIGIN      
        1 agctcacgcg aatcccgccc gcaccggtca cgaggagata gctccccacg atcgccagca
       61 gaatcgctcc cgatttcaag gcagtcagag gttccttttc aacgagccag gcatgcatgg
      121 cgacgaatac cggcgctgtg tattgaagaa agagcgctgt ggatacgtct gtcaggctga
      181 tcgtgacgta atacgtgaac tgcgccgatg tcatcgccag gcccagtacg atgagcctgc
      241 cggtctgagc gcgtggaacg cgaagagata cccgcgtgag tctgaggatc aatagcagag
      301 ttacgaacgc accggtgagg cggatcgcga gcagcgtcca gggttcaatc tgccggttga
      361 agagcatctt ggcgaggact cccgagattc cccacagtgc ggccgcacac atgaccgcta
      421 gatagccttg gagtcgtgcc cagaggtggc cgtgcacttc aggaaatcag cgtggaaaca
      481 gccagcgtga agcctgcgag catgtccgac ccggatgttt ccccgaacgc catcacccgg
      541 cgagtcgctg aagcaatggc tgcccgatct ccgttgggaa gcactcgcaa gagccggtgc
      601 caagcctctc cggcttctcc gcgagccgcg gccatgaggt atgcttcgct gatgcgcgtg
      661 gtgcgcccgc gcactcgcgt gatgatcgcg gttcggagtt cactaggttc atgggggagc
      721 actgagaggc atatgagcgc tccgaccagc acatcgtcgc ctgagggtgt gagcccgctg
      781 ccgagtccgg ccaggtgctg cgcggcctct ccggtggaga ccggagaacg gtgcattagt
      841 ccctcttcga ggaggttcat cccactgcgc gcgcggacac tcagacccgc cggatctgtg
      901 cgatacgtcg cgaagctgcc ctcaggggtt tccccaagaa gaatctcctt cagaaaattc
      961 aagttggcac gcaccgaaga aaggtaactt ctgccccatc ttgcgagttg cggttcccag
     1021 atcgatgctt gctggaccgt gagctcgact gcgttgccga tgaggatacc cgcactggag
     1081 ttctccaccg tttcgccgac cggtagatca tagaattgga agtcgccggt tgcctgcacg
     1141 agcaggttca gcggaccatc gaggagttcg ggaagaacca gagcgacgac gtggccgtcg
     1201 agatctagat agcaactccg gtcaaaggtc gcgaggaccg tcccgctgtg aggtatctga
     1261 agcaggtcgt gtacgtgtct gctcacggaa acggctttga gacgagccga gagagcactt
     1321 tgagccatgt gtgattaccg cggtttgccg cttgcgcgtt tgcgcgtttg catcttcatt
     1381 cgtcctgccg catcctccac cgcgtccacg atgccctggt atccggtgca cctgcagagg
     1441 tttccggcca gcgcttcccg gatctgatct ttactcggat cgggtgtttc actgagaaac
     1501 gcgacggcgg tcatcaagaa tcccggcgtg cagaagccgc actgcagacc atggtggtca
     1561 cggaacgcct cctgaatcgg gtgaaggcgc tcgggcgtac ccagcgcttc gaccgtcgtg
     1621 atctctaatc cgtccgcttg gacggcgaac atcaagcaac tgcgcacggc gcgtccaccc
     1681 aggatgatgg tgcaagcgcc gcagactccg tgctcacatc ctacatgcgt accggtcatc
     1741 gcaaggtctt cgcggatgaa gtcgacaagt gtcttccgca cgtcgacgtt ccccgtatac
     1801 gcgcggtcgt tgacccggag tctgatgtct ttccgatcga tgagttcagg agtttgcgat
     1861 cttctagatg tcatcgcgtt cctgtggctc gctggtgggc tagccgcaat gcgcgaacgg
     1921 ccagcacgcc tgccacgtgt ttcctgtact ccaccgacgc atggacatcg ctgtcgggat
     1981 ccaggcggcc ggcgacgatc tgacggactt cactcaggat cttagatgtc aggcggttgc
     2041 cagtgactgc tgcctcggcc tccgggatcc gaaccggtcc tggcccgaca cccgtaaagg
     2101 ccagccggca atccttgatc gaatcgccgt ccgcagagag gatggcggca acgcccgcca
     2161 gcgcgtaatc accgtgacgg cgcgccacct cgagaaaggc agttcccatc ttctgatgtg
     2221 cggggaacct gacttccacg agtaactcat cgggtccgag cgccgtcgtg agcagggcga
     2281 ggaagaattt ctccgactcg acaactcgcc ttcctttcgg ccctcgaaca accatccggg
     2341 cgcccagcgc cgcagcgaca gcgggcaact cagcagacgg gtccgcatgg gcgatgctgc
     2401 cgccaatcgt tccccggttg cggacttgtg ggtggccgac ataccccagg gcttcggcca
     2461 ggagcgggag tcggcgcgtc accagcggtg atagttcagc gtcccgctgg cgggtcatcg
     2521 ccccaataac cacgtcaccg ttgtcttcct ttatatagga gagctcaccg accgcgttca
     2581 gatcgacgat gaccgatggc cgggccaggc gcatgttgag caggggaacc aggctctgcc
     2641 cgcctgccag gagcttgccg tcctgcccat gctcgcgcag cagcgcgagg gtttctaata
     2701 cggtctccgg cgcgctgtat ttgaaagggg caggcttcat cgagagtcgt agttaggtgg
     2761 cgcccaccag tgtcatcgcg aagcgggcgg cggcgcacag aggctgagct gcggcgaggc
     2821 cgggggaatt ttgcttggtg ccggggtcaa tgcaggctgg ggcgaccccg tctgtatgat
     2881 ctccaagatg cggttcgggc tgagcggcat ttcggtgata cgcacgttcc attctgacag
     2941 cgcttcgtcg atcgcctgcg cgaagagcgc ggggaccggg atgacccctg cttcgccagc
     3001 gcccttaacg cccagcggat tcagcgggga cggcgtttca atatgaccca cttcgacctc
     3061 gggaatgtct gtcgcggtcg gcaggaggta atccatgaac gtctgggtca agagctgccc
     3121 ggtttcatcg tagacgagtt tctcatagaa ggcgccgccg atcccctggg ccaccccgcc
     3181 gcggacttgg ccctccagaa tcatcgggtt aatcaccttc ccgcaatcgt gcacgacgac
     3241 gtatttgagg atcttgatca tgccggtttc tgggtctacc tcgaggatcg cggcgtggac
     3301 gcccgctgcg aaggttccgc ggggcggcgc aaagtaccgg ctggcttcca gtccgggctg
     3361 ttcccactcg tcgggaatcg ttccgcgcag aggattggcc acggccgcaa cctctgcgag
     3421 cgtgagtgac tttccgggga cgccgcgtac gaataccttg ccgtcggcca actcgagatc
     3481 gttgacgctc gcctcgagcg tggttgccgc caccttgaac gttttctcac ggaccgcctg
     3541 cgccgcgagc gcaaccgcgt tgccggcgac gaccgcggcc cggctggcaa atgtgccggt
     3601 gccccagccg aacgcaccgc tatcgcctgt ggtgatgatc acatcgtcaa taggaacggt
     3661 gagcgcgtcg gcgatgatct gggcgaagga cgtgaagtgc ccctggccct gtgtcccaac
     3721 gctggtcgca gcatagacct ttcccgaggg ctccacggtg acccggcagc cctcgtatgg
     3781 tccgatgcct gacccttcca cataacacgc gattcccaat cccacatggc ggccttcgcg
     3841 ccggtgctgt tcgcgcgact ccggccagcg atcatagcca atcatctcca gcgcgcggtt
     3901 caggagcagc gggtaattac cgctgtcgta gatgagcggc gcattgtcct ggtagataag
     3961 gccgacgtca tagggaaatt cgtgcggctg aatcaggttg cgccggcgta cctcggcccg
     4021 gtcgaggccc agctcacgtg cgactcggtc gagcaggcgc tccatcacga agacgccgtg
     4081 gggcctgccc gcgccgcggt aggggctgac gatcgtcttg ttcgtgaaga cggctttgaa
     4141 ctcgcaatga tagttcggaa tcttgtacgg tccgggcagc gtcgtgccgg cgacaatggg
     4201 gacaataatc ccgtacggga tgtatgcgcc agaatcgtac aggaatacgg tttttacacc
     4261 gaggatgcgt ccctggtcat cgaccgcgat ggccgcgtcg tgaatctgca cgcgctcatg
     4321 gttcatcgcc acgaagttct cgcgacggtc ttcgatccac ttgaccggcc gatccaactg
     4381 catcgcggcg aaggggacga gcacctcctc cggatagaac atcatgatct tggggccgaa
     4441 cccacccccg acgtccggcg cgatgacccg cacgtttgcc tgcgggagtc cgagcagtcg
     4501 ggcgagtcca ttccggatcg gaatcggcgc ctgcgttgaa tcccagaccg tcaactgccg
     4561 cgttttccca tcccactgcg cgaccacggc gcggcactcc atcggtgccg ccgtgccacg
     4621 atcgatcacg aaccggtccg tgaacacgcg cggcgcgctc gcgaatgccg cctccgcgtc
     4681 acccacgcgc tgcgtataat gcgcgcatac gttggtgcca atgtccccgt gcaccaggga
     4741 tgcgccgtcc cgggcagcct gttctaggtc cacaacggga ggcagaggct cgtattctac
     4801 gtcaatcagg tcgagcgcat cctcagcgat gtatcggctc tccgcgacca cgaacgcgac
     4861 cgcctccccg acgtgacgga cggtgtcagg gaccagcgcg tattgcgtct tatgatggat
     4921 cagtgacggg tgcggaatga gtttggggag cggtccggcg atcgcctcgg acaaatctgc
     4981 gtttgtgtag acggcgatga cgccccgcag cgcctgcgcc ttcgaaatat
//
LOCUS       DBNGSB010000117         2683 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2995667, whole genome shotgun sequence.
ACCESSION   DBNGSB010000117 DBNGSB010000000
VERSION     DBNGSB010000117.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2995667"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="AC159B_03190"
     CDS             complement(<1..453)
                     /locus_tag="AC159B_03190"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019714901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR3376787.1"
                     /translation="MAEIRYIDAINQAHHEEFARDERVVVMGEDVGRKGGVFGATMGL
                     SEKFGEDRVIDTPLAESSIAGVAIGMSINGLLPIAEFQFADFIHPAFNQIVSEAARMR
                     YRSNNAFGCPVVFRAPYGGSHGTALYHSQSIETLYAHVPGLKVVAPYTP"
     gene            complement(466..1488)
                     /locus_tag="AC159B_03195"
     CDS             complement(466..1488)
                     /locus_tag="AC159B_03195"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018395744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HIR3376788.1"
                     /translation="MSAMEAKPRVTPARHKTLGLSDKDVLEMYYYIALARALDDRMWV
                     LQRAGKAMFVISGPGHEGCQVGAIWPMDKKHDWFVPFYRSIAACLVNGRSARDILLGL
                     LARAEDPSSGGRQLPGHYGHPRVKIPSTSSPVGTQYPHAAGIAYASKIRKTGEVTMVA
                     IGEGGTSQGDVHEAMNWAGIYKLPVIFVVENNMYAISVPLHKQVAGGNVAARAAGYGF
                     PGVATDGSDVLECYRVSKEAYDRAKRGDGPTLLEFRVPRLGSHSSDDQQERYRTKEEI
                     DGDRRRDPLQTFRIYLESVGLMDEGKAKEIAGRVKREIDEATDYADRSPLPEPESAIR
                     HVFYDE"
     gene            complement(1522..>2683)
                     /gene="lpdA"
                     /locus_tag="AC159B_03200"
     CDS             complement(1522..>2683)
                     /gene="lpdA"
                     /locus_tag="AC159B_03200"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HIR3376789.1"
                     /translation="AVHKRKARVVDQLHKGVQFLMRKNKIDVFNGEGRFLTPQKISVA
                     LQDGSETELQAKHTVIATGSVPRSVPGIAFDNQRIIDSTGALALTEVPKSIAILGAGP
                     VGVEFASLFRAFGSEVTIIELLPTLVPLEDEEIGQTLEKSFARRGIKVITNATAQKAE
                     VSNERVRVTIAKGNETSTLEAEYLLVAVGRTPLTEGLALDEAGVALEKGAVKVDTGLK
                     TSADHIYAIGDVITGQAPYRLAHVASDESIAAVETIAGAEVHAIKYETVPRPTFSIPQ
                     VASVGMSERQAKEQGHEIKVGRFSFQANSKASIEGAREGMVKIIVDAKIGELLGVHMI
                     GPTVTELLAEGVAVKYLEGTVEELGSAVHSHPTLAEALKEAALDAMGRVIHA"
BASE COUNT          452 a          894 c          787 g          550 t
ORIGIN      
        1 gggcgtgtag ggtgccacga ccttgagccc aggcacgtgc gcatacaacg tttcgatgct
       61 ctgagaatgg tatagcgcgg ttccgtggga tccgccgtag ggtgcacgga acacgactgg
      121 gcagccaaag gcattgttgg agcggtagcg catgcgggcc gcctcgctca cgatctggtt
      181 gaatgccggg tggataaagt cggcgaattg aaactcggca ataggcagca gaccgttgat
      241 cgacatgccg atagccacac cggcgatcga cgactcggcg agcggagtgt cgatgacgcg
      301 gtcctcgccg aacttttcgc tcagccccat cgtggcgccg aacacgccgc ctttgcgccc
      361 gacatcctcg cccatcacga cgacgcgctc gtcgcgagcg aactcctcgt ggtgggcctg
      421 gttgatcgcg tcgatatatc ggatctccgc catcaaccga tcctcctact cgtcatagaa
      481 tacgtgccga atggcgctct ccggttcggg gaggggcgac cggtcggcat agtcggtggc
      541 ctcatcgatc tcgcgtttga cgcgcccggc aatctccttg gctttcccct cgtccatcag
      601 cccgacactc tcaaggtaga tgcggaacgt ctgcagtggg tcgcgccggc gatccccgtc
      661 gatctcttcc ttcgtgcgat accgctcctg ctgatcatcg ctgctgtgag agcccagccg
      721 gggaacgcgg aactccagca gcgtgggccc atcaccgcgc ttcgcgcggt catacgcctc
      781 tttcgaaacc cggtagcact ccagtacgtc gctgccgtcg gtggccacac cggggaatcc
      841 gtacccggcg gcccgagcag ccacgttccc accggcgacc tgcttatgca gcgggaccga
      901 aatcgcgtac atgttgttct cgacgacaaa aatgacgggc agcttgtaga ttccggccca
      961 gttcatcgct tcgtgaacat ctccctgact ggtcccaccc tcgccgatgg cgaccatcgt
     1021 gacctcaccg gttttcctga tcttcgaagc atacgcgatg cccgccgcat gcgggtattg
     1081 cgtgccaacc ggactactgg tggagggaat cttcacgcgt ggatgaccat agtgaccggg
     1141 caactggcgg ccgccgctgc tgggatcctc ggcgcgcgct aaaaggccga gcagaatatc
     1201 gcgtgcgctc ctgccattga cgagacacgc ggcgatggaa cgatagaacg gcacgaacca
     1261 gtcgtgcttc ttgtccatcg gccagatcgc gccgacctga cacccttcgt ggcccggccc
     1321 ggagatgaca aacatggcct tccccgcacg ctgcagcacc cacatccggt catcaagcgc
     1381 gcgggccagc gcgatgtagt agtacatctc gaggacgtct ttgtcggata gccccagcgt
     1441 cttgtgccgc gccggtgtca cccggggttt ggcctccatc gcgctcatcc cgttatcgcc
     1501 tctcctactc gcattcccgc ctcacgcgtg tatgactcgg cccatagcgt caagcgcggc
     1561 ctccttcaga gcctccgcga gggtcggatg cgaatgcacc gcggacccaa gctcttctac
     1621 tgtgccttcg aggtatttca ccgccacacc ctccgccagc aattcggtga cagtggggcc
     1681 gatcatgtgc acgccgagca attcgccgat cttggcgtcg acgatgatct tgaccattcc
     1741 ctcgcgcgcg ccctcaatcg acgctttgct gttcgcctga aagctgaacc ggccgacctt
     1801 gatctcgtgg ccttgctctt tggcctggcg ctcgctcatc cccacgctcg ccacctgcgg
     1861 aatcgagaac gtcggcctgg gcaccgtctc atacttgatc gcgtgcacct cagcgccggc
     1921 gatcgtctcg acggctgcga tggactcgtc gctggcgaca tgagccagcc ggtacggcgc
     1981 ctgtcctgta atgacgtcgc cgatcgcgta aatgtgatcc gcggaggtct tgagcccggt
     2041 gtccactttc acggcgcctt tctcgagcgc cacgcccgct tcgtccaggg ctaatccctc
     2101 tgtcaggggg gtgcggccga cggcgacgag gagatattct gcctccagcg tgctcgtctc
     2161 gttgcccttt gcgatcgtca cccgcacgcg ttcattgctg acctcggcct tctgtgcggt
     2221 tgcgttcgtg atgactttga tcccgcgcct ggcgaacgac ttctcaagcg tctgcccgat
     2281 ctcttcatct tcgagtggca cgagtgtcgg cagcaattcg atgatcgtga cctcgctgcc
     2341 aaacgcgcga aacaagctgg cgaactccac gccgaccggc cccgcgccca gaatggcgat
     2401 cgacttcggg acctcggtca gcgcaagcgc ccccgtgctg tcgatgatgc gctggttgtc
     2461 gaacgcaatg cccgggacgc tgcgggggac agagccggtt gcgatcaccg tgtgtttggc
     2521 ctggagctcc gtctccgatc cgtcctgcag cgcgacgctg atcttttgcg gggtgagaaa
     2581 tcgaccctct ccgttgaaga catcgatctt gttcttgcgc atgaggaact gcacgccttt
     2641 gtgcaattga tcgaccacgc gggccttgcg cttgtgcacc gcg
//
LOCUS       DBNGSB010000118        16445 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_543660, whole genome shotgun sequence.
ACCESSION   DBNGSB010000118 DBNGSB010000000
VERSION     DBNGSB010000118.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 16445)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16445)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16445
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_543660"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="AC159B_03205"
     CDS             <1..431
                     /locus_tag="AC159B_03205"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3376790.1"
                     /translation="RMVGLTGFDHHYPHELSGGMRKRVSLIRTLIYDPDIILMDEPFG
                     PLDAQTRVILQDELLKLWEGTGKTVVFVTHDLVEAIALSDEIALFSRAPGRIKHVYEV
                     PLERPRDVFRIHTHPNFAAFYDRLWRDLKEEIVAVELGVS"
     gene            434..1309
                     /locus_tag="AC159B_03210"
     CDS             434..1309
                     /locus_tag="AC159B_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3376791.1"
                     /translation="MGVREAAAPGRLLEPRGDRLLREVEQFEQAQDRQNRRNTTLGRI
                     AFAALFLLAWEVASGRVLDQFFVSKPTHIIASLWAMVVKEQLFYHLQFTVIEALTGYL
                     IGAVGGLAVGFVLARSDAVYRIIEPFVVAFYGIPRIALAPLFILWFGIGITSKIAVAA
                     IMVFFIVFINTVAGIRSAPTQLLQIARVMGASEWDLVLKVTFPAATPFIIAALQITVP
                     QAMIGAIVGEFISSNRGVGHLISRAAGWLDTPGLFAGILTLLIVVLVMNLGITSLGNY
                     LMRWNPKHGNMRDLQ"
     gene            1341..2363
                     /locus_tag="AC159B_03215"
     CDS             1341..2363
                     /locus_tag="AC159B_03215"
                     /inference="COORDINATES: protein motif:HMM:NF024772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3376792.1"
                     /translation="MSTQWWARVTVMLAAVILAVSGGAMAQTPRVKIREAHTGTLFMA
                     PVYIAEAAGFMSEEGIDLELTEVESGALGIAALVSGQVEFFDADPFQAVQLRRQGRQI
                     TFIYNLTKRVTLDMVMHPEVARARGVSRSMPIQQRFAALRGLKLGVTRPGAATDVYMR
                     YYLKRAGLNPDRDAQIISAGGGGPLLAALRTRQIDAFHLSAPTPYQAEQDGFGVIVIK
                     ASAGDVPELDNFLYTGIAVHSVYANRNGDVLRRWIRAVNKANQLMRRDQAAAVTHLRK
                     HFPRQDPAVLALALREIIPSLSEDGRMNVQMMQKHIDFMFEDGQIPFKPSASENVLWT
                     NRYNGR"
     gene            complement(2308..2949)
                     /locus_tag="AC159B_03220"
     CDS             complement(2308..2949)
                     /locus_tag="AC159B_03220"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HIR3376793.1"
                     /translation="MSTRSAAVSRCEWAEGSPLLRRYHDREWGVPVHRDRKHFEFLIL
                     DAFQAGLSWNLVLRKRPALRRAFANFNPRRISQFTGREVRRLMADPGIIRNRGKIEAT
                     IVNAQRFLDVQREFGSFDRYIWSFVSDRPILSRHRQMSDLPAKTPVAETMSRDLKLRG
                     FKFVGPTICYAYMQAAGLVNDHTLGCFRYGQVAPRVTSRYTGSSRGRSRSPTA"
     gene            complement(2946..3836)
                     /locus_tag="AC159B_03225"
     CDS             complement(2946..3836)
                     /locus_tag="AC159B_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012660611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR3376794.1"
                     /translation="MRIATYQGAGESRLGFVVNDRIVDLQALARRKKLSVPKDMITLI
                     EETPTLALRALGREADAWVRAGRESSPLSKAKLLAPIPRPSKNIFCMGRNYAEHAREG
                     GVAPPEVPVFFTKSPTTVIGHEMPVIHHRVTQSLDYEVELVAVIGRRGRDIPAAKALD
                     YVFGYTIMNDVTARDLQRRHQQWFKGTSLDTFAPMGPWIVLRSAIPDPQKLRLSMRVN
                     GEVRQNSTTASMLFSVAQLIETLSAGLTLESGDLLATGTPEGVGMGFTPPKWLKVGDT
                     LEAEIEGIGVLRNPVGAPAR"
     gene            4067..5182
                     /locus_tag="AC159B_03230"
     CDS             4067..5182
                     /locus_tag="AC159B_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR3376795.1"
                     /translation="MAKVTVLAGTTNGMYAFESDARRAKWKKRGPYLKGVSVNHFAYE
                     PKSKTLYAATLDQGVMTSKTLGRSWSALSDGLPIRKVWSVGVNPKDSSELWAGTHFSY
                     LFRSKDRGRTWSLLEGYLKAPGKEDRWGDWAFGTIGNSLHGIHFDPADPKRMIVVSST
                     NHGAVRTADGGETWEYARNGVFESCPSAHQSALGKPPDEQAQSVKEHLEQVHSCTHRI
                     SIVPAAPNVVYRQQHCGVYRSDDFGSTWRDISSGLPERHGFPLAVHPKDTQTAFVVPA
                     YQGKCDKHNSCIIGALDVYRTRTGGRSWEKLSEGLPRDVHCVVLRHGMDIDRLQTPGV
                     YFGTTTGDVYASPDSGDSWVRLATRLPRVQGITAIVA"
     gene            complement(5192..5722)
                     /locus_tag="AC159B_03235"
     CDS             complement(5192..5722)
                     /locus_tag="AC159B_03235"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008291052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HIR3376796.1"
                     /translation="MSPKISITVAMSENNVIGRNSDLPWHQATDLKRFKRMTTGHTVI
                     VGRKTHEAIVWRLGRPLPGRRTIVLSRNPDFTAPGCEVVQTLSVAIARVNGEEEVFVI
                     GGAEVYRSALEYADHIYLTRVHATVTGDAIFPGVDFSDWTLISREKHAGDADNDYDYS
                     FEVWERAPVTASVSVP"
     gene            complement(5738..6655)
                     /gene="thyA"
                     /locus_tag="AC159B_03240"
     CDS             complement(5738..6655)
                     /gene="thyA"
                     /locus_tag="AC159B_03240"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: protein motif:HMM:NF012524.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HIR3376797.1"
                     /translation="MYRPFEQRVPDRQYRDLLLRILTGGEEVPTEHGVPALKIIGHAL
                     RFPFANGFPIITERDVLSRPQNGRRPSPFHQAIGEICAFINGAQTQQELEQFGCFWWN
                     RWVTAEHCEEFGLAAGDLGPGSYGAAFRRFPTAGGDPFDQVKHLIDQIRTRPAARHHE
                     LSPWIPNFVLAANGARRHVVVPPCHGWVHVHVNTETRELTLTHRQRSADVPVGLAFNF
                     IHYAALALMIGQVTGYRPKELVYWIDDAHIYLNQIEDVQVMLATEPQRFPTVNVDPDI
                     TDIFAFRHHHFAVTDYYPRLSRRKIPTPV"
     gene            6927..7502
                     /locus_tag="AC159B_03245"
     CDS             6927..7502
                     /locus_tag="AC159B_03245"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR3376798.1"
                     /translation="MKDFEMSVRGRLAAGKRREAPDSPGRRAAVLVPLYDTEEGPYVL
                     FTKRTDAVEHHKGQISFPGGAADPDDPDALSTALRETHEELGIAPHDVKVLGALDDVP
                     TVVSGFVITPFVGIIPHPYPLRVNPVEIAEVLFVPLSIFRDADKMRVEERDREGERVT
                     VYFYYYGPHEIWGATARIMKGFIDTVFGDSA"
     gene            7730..8050
                     /locus_tag="AC159B_03250"
     CDS             7730..8050
                     /locus_tag="AC159B_03250"
                     /inference="COORDINATES: protein motif:HMM:NF024803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR3376799.1"
                     /translation="MKQLTQPLFTVSVASSLVGVSSQTLRKWETAGLVAPVRDRSTRR
                     RLYSWAEIERMQQIRYLLMRRRIPLREVKPMLRALAARRRPGIVPDDRRKRAPLSVPI
                     ALPR"
     gene            8122..9288
                     /locus_tag="AC159B_03255"
     CDS             8122..9288
                     /locus_tag="AC159B_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HIR3376800.1"
                     /translation="MTRVYADHGATTPTDPRVVEAMLPFFSEKYGNASSVHQWGQDAR
                     EAVDAARELIAAGIGAKPAEIVFTSGATESDNFAVFGAAYANEKKGRHVVTSSVEHHA
                     LLESCRFLENRGWEVTYLPVDRPGVVDPDDVRRSMRDDTVLVSIMHANNEIGTTEPIA
                     EIGRIVKERGALMHTDAAQTFGIVPVDVDDLCVDLLSISAHKRYGPKGIGGLYIRKGA
                     HVSRIQHGGSHERNRRGGTQNVPAIVGFGKAMEIALASMGEESARLAQMRDGLTDGLA
                     RIDGAHLNGHPSRRLPGNVNFSFEGVDSESVLMALDFQGVAASSGSACTSGSLEPSHV
                     LAAIGRPPHIAAGTIRLSLGRPTTPDDVAYLSRVVPDVISSLRGASRKVSRASR"
     gene            9351..9707
                     /locus_tag="AC159B_03260"
     CDS             9351..9707
                     /locus_tag="AC159B_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HIR3376801.1"
                     /translation="MTGSYVPILVHFALVVALTALLLSAHALLGRGRPLPGKYEAYES
                     GVWPIGSARERVPIRYYLIAMLFLLFDIEAVFLYPWAVVARELRIFGLIEIIVFIGVL
                     GLGLVYAWKRGALEWQ"
     gene            9773..10228
                     /locus_tag="AC159B_03265"
     CDS             9773..10228
                     /locus_tag="AC159B_03265"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HIR3376802.1"
                     /translation="MRALEGNILTSTVDRFVAWSRKNSIWPVQFGLACCAIEMMSAAA
                     SRFDIARFGSELFRASPRQADLMIVSGRVSQKMAPVVQHIYEQMLDPKWVIAMGDCAS
                     CGGVFNNYALVQGVDKLIPVDVYVAGCPPRPEALLYGFLKLQELISARS"
     gene            10225..10737
                     /locus_tag="AC159B_03270"
     CDS             10225..10737
                     /locus_tag="AC159B_03270"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012549.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HIR3376803.1"
                     /translation="MTRDPIDMLTQQLGATVLEQTRFRDDLTLVLSTDGLRAALQSAR
                     AAGFSLLTDLTAVDRYPATPRFEVVYHLTAPDARAKIRLKARIAETGAVPSATPVWPG
                     ANWLEREVFDLYGIRFDGHPDLKRILMPDDWEGYPLRKDFPLTEEPVQFFGHTPKVPS
                     EIIPKSPPKT"
     gene            10776..11918
                     /gene="nuoD"
                     /locus_tag="AC159B_03275"
     CDS             10776..11918
                     /gene="nuoD"
                     /locus_tag="AC159B_03275"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HIR3376804.1"
                     /translation="MGPQHPATHGVLRLLLELEGEVILSTEPVIGYLHTGFEKEMETR
                     TYHQNIVFPPRIEYLATFIEEQAYVLAVEKLLGIRAPKRAETIRLMLAELSRIASHLV
                     WLGTSAIDLNVSSVFMYCFRDREQILDMFEMVSGQRMMHGYFRIGGLQWDLPAGFTDS
                     VRTFLEGFPGRIDEFETLLTDNLIWRQRTEGVGVLSAEDALRYGCTGPMLRGSGVNYD
                     VRKAFPYGGYDEYEFDVPLGEHGDSFDRYKIRVEEMRQSRRIILQALNRLADGPIAID
                     DRKISLPPRPELVRSMEAVIHQFKLVSEGINPPVGEVYAAVESPRGEKGYYIVSDGSN
                     RPTRVRVRAPSFSNLQALPVMVYRRTIADVVVVIASIDIVLGDVDR"
     gene            11915..12433
                     /locus_tag="AC159B_03280"
     CDS             11915..12433
                     /locus_tag="AC159B_03280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018234088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE family
                     protein"
                     /protein_id="HIR3376805.1"
                     /translation="MNAGNGERGTGNGISESAKVEIRRLIAQFPQRQSALLGALFVAQ
                     DEVGHLSPEAIRDVAEAMELAESEVTSVASFYHLYQFKPAGRHLIQVCTNLSCMLMGC
                     GRILEHLKDRLRIDVGQTTPDGRFTLKTAECLAACELAPMMHIGDDRFGPLTPEKVDE
                     ILGRVVSRTSNG"
     gene            12430..13704
                     /gene="nuoF"
                     /locus_tag="AC159B_03285"
     CDS             12430..13704
                     /gene="nuoF"
                     /locus_tag="AC159B_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017569255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HIR3376806.1"
                     /translation="MSEPFLLKYINEPDQGDLEAYVSRGGYEGMRRGLSMTPEAVIDE
                     VDKSGLRGRGGAGFPTGRKWKFIPRDPAVTKYLVVNADEGEPGTYKDRTLMEGDPHRL
                     IEGIIIGSFAIGARQAFIYLRREFAPARAALTKAIAQARAGNYVGRNILHTGYDLEII
                     LATGAGAYIAGEETALLESLEGRRAMPRLKPPFYPAVKGLYMQPTAVNNVETLHHVAW
                     ILAKGHEWYKAQGSPILLSVSGHVERPGVYEVPLGTTIRSTIDLAGGMRRGRKFKACF
                     PGGSSSAILTGAYLDTPLDYDALPKVDVPGTMLGSAAVIVMDETACMVEVIARTVEFY
                     RDESCGKCTPCREGTVWLGQVFHRILHGQGRPEDLDLLGGIAKGMTGTCFCPLGESVP
                     PSLNASLRFFRHEYDHHIKTGTCDVKFHRASP"
     gene            13701..16154
                     /gene="nuoG"
                     /locus_tag="AC159B_03290"
     CDS             13701..16154
                     /gene="nuoG"
                     /locus_tag="AC159B_03290"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HIR3376807.1"
                     /translation="MSDQPRVTLAIDGTPITVPKGTTVYQAAKQSGIEIPIFCYHDRM
                     PPLGACRMCLVKVEKMAKLQTSCTLEALEGMVVDTATADVKAGQEAILEFLLINHPLD
                     CPICDKGGECPLQDQTFKFGPGRSRFVEAKRDFAKPVSLGPVLVLDRERCILCWRCVR
                     FGEIVAGDDALKGFERGFHSEINTPFTLPVRSKFIGNTIEICPVGALTAKSYRFIARP
                     WDNTPVTSTCTLCGVGCAVEFDVRGGKIARTRPSEDPAVNDIWLCDLGFFGHTFVHHE
                     GRLRRPLIKREGGFVEATWDDALALVASRLERAGARVAMLGGARLTNEDAYVATRFFR
                     SVVRTAHMDHRVDALARGPDLSMSWGMTTAIEELARADCLLLVGCDITETYPVIWLRM
                     KQAVEHGATLITVGTRALEIEASVRLPFLHRQAHGAEIVRALGESVRGSPPGGPVEGI
                     DRDAIAAAAAALSAASQPVVLVGREAMDAPDGWGILLATGEICRKIGVDLNVMRGKGN
                     AYGASLAGILPDAGPGGRALEEAAADLKALWGEVNTDKGMTAPEIIEAAAAGGLDMLY
                     VAGADPANDVPDRERWRAARARVPFVVVQDAFLSETAQSADVVLPTLVAPEKNGTVTS
                     IEGRIRRLRAAAHGPGEARGDWTIFAALAVKLGKTIGYSSWEEIFDEMRGLIPALAFD
                     ARLPMRHPPIDAGPDEGRHAIEEAKGYPYALILGDVLFDRGALMTRSPTIADLAGEPW
                     ALIHPNDAAALGAVQDASVVISSRRGSIAVRARISSSIRPGEVFLPRGYDAAPANRLV
                     DAREMLTYVQVRALVPATT"
     gene            16223..>16445
                     /locus_tag="AC159B_03295"
     CDS             16223..>16445
                     /locus_tag="AC159B_03295"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HIR3376808.1"
                     /translation="MTLLERRLLGKFQIRYGPNRVGPFGLLQPLADGIKLIFKEDFMP
                     ARVDVLVYWLAPAISMVTALFVYAVIPFGP"
BASE COUNT         3005 a         5021 c         5291 g         3128 t
ORIGIN      
        1 ctcggatggt cggtctgacc gggttcgacc atcactatcc gcatgagctc tccggcggga
       61 tgcgcaaacg tgtgtcattg attcggaccc tcatctatga ccccgatatc atcctcatgg
      121 acgagccctt tggaccgctg gatgcgcaga ctcgagtgat cttgcaggac gagttactca
      181 aactgtggga agggaccggc aagaccgtcg tgtttgtgac gcacgacctc gtcgaggcca
      241 ttgcgctgtc cgacgagatc gcgctcttca gccgggcgcc ggggaggatt aaacacgtct
      301 acgaagtgcc cctggaacgc cctcgggacg tgttccgaat ccatacgcac ccaaacttcg
      361 ccgccttcta cgatcgtctg tggcgcgatc tcaaggaaga gatcgttgcg gtcgagttgg
      421 gggtgagcta gtcatgggcg tgcgcgaagc ggcggcgccg gggcgtctac tcgagccccg
      481 tggagaccgg ctcctccgtg aagtcgagca gtttgaacag gcgcaggacc gccagaaccg
      541 gcggaatacc acgctgggac gaatcgcgtt cgccgcattg ttcttgttgg cctgggaagt
      601 cgcttccggc cgcgtgctcg accagttctt tgtcagcaag ccgacgcata ttatcgcctc
      661 tttgtgggcg atggtcgtca aagaacagct cttctatcat cttcagttca ccgtcatcga
      721 ggcattgacg ggctatctca tcggcgcagt cggcgggctg gccgtgggat ttgtgctcgc
      781 ccgatccgac gccgtctatc gcattatcga gccgtttgtg gtggccttct acgggattcc
      841 acgtatcgcg ctggcgccgc tgttcatcct gtggttcggg atcggcatca cgtcgaagat
      901 tgccgtcgcg gccatcatgg tgttcttcat cgtgttcatc aatacggtcg cggggatccg
      961 gtccgcgccg acgcagctcc tgcagatcgc gcgtgtgatg ggtgcctccg aatgggatct
     1021 ggtgctaaaa gtgaccttcc ctgcagccac gcccttcatc atcgcggccc tccagatcac
     1081 ggtgccgcag gcgatgatcg gcgcgatcgt cggggaattc atcagcagca accgcggggt
     1141 cgggcacctg atcagccgcg cagccggctg gctcgatacg cccggcctgt ttgccgggat
     1201 tctcacgctg ctgatcgtcg tcctcgtcat gaacctgggt atcacgtcgc tcgggaacta
     1261 tctgatgcgc tggaatccga agcacggcaa catgcgagat cttcaatgag ttatacgcgt
     1321 gtgaggaggg gatgacagga atgagcactc agtggtgggc gcgggttacc gtgatgcttg
     1381 ccgcggtcat actcgccgtc tcaggcggcg ccatggcaca gacgccgagg gtgaagatca
     1441 gagaggcaca cacgggaacg ctgttcatgg ccccggtgta tatcgcggaa gccgcgggat
     1501 tcatgtcgga agaagggatc gatctcgagt tgacagaggt cgagagcggc gcgctcggca
     1561 tcgccgcgct ggtatctggg caggtcgaat tcttcgacgc tgatccattc caagcggtgc
     1621 agctccgccg ccagggcagg cagatcacct tcatctacaa tttgaccaag cgcgtaacgc
     1681 tcgacatggt aatgcatccc gaggtcgccc gcgccagagg cgtcagccgg agcatgccga
     1741 ttcaacagcg cttcgcggcg ctgcgcggac tgaagttggg agtcacgcgg cccggcgcgg
     1801 ccaccgacgt gtacatgcgg tactacctca agcgcgccgg gttgaatccg gaccgcgacg
     1861 cgcagatcat ctccgccggc ggaggcgggc cgctgctggc ggcactccgc accaggcaga
     1921 tcgacgcgtt ccacctgtcc gcgcccaccc cataccaggc ggagcaggac gggttcgggg
     1981 tgatcgtgat caaggcatct gccggcgacg tgcccgaact ggacaacttc ctctataccg
     2041 gcatcgccgt acatagtgtc tacgcgaaca gaaatgggga tgtgcttcgc cgctggatcc
     2101 gtgctgtgaa caaagccaac cagctcatgc gccgggatca ggccgcagcc gtcacgcacc
     2161 ttcggaagca ctttccgcga caggatcccg ccgtgctggc actggcgctg cgcgagatca
     2221 tcccatcgct gtcagaggat ggacggatga acgtacagat gatgcagaag cacatcgact
     2281 tcatgttcga ggacggtcaa atcccgttca agccgtcggc gagcgagaac gtcctctgga
     2341 cgaaccggta taacgggagg taacgcgagg ggcgacctgc ccgtatctga aacagccgag
     2401 ggtgtgatcg ttgaccagcc cggccgcctg catgtacgcg tagcagatcg tcgggccgac
     2461 aaacttgaac ccgcggagtt tgaggtcgcg gctcatcgtt tcggccacgg gtgtcttggc
     2521 cgggaggtcc gacatctgcc ggtgccggct gaggatgggc cggtcgctga cgaacgacca
     2581 gatgtagcgg tcgaatgatc cgaactctct ttgcacgtcc agaaaccgct gggcattgac
     2641 gatggtggct tcgatctttc cgcgattgcg gatgataccg ggatcggcca tcaggcgccg
     2701 gacctcgcgt cccgtgaact gtgaaatacg gcgcgggttg aagttcgcaa acgcgcgccg
     2761 gagcgcggga cgtttcctga ggaccaggtt ccaactcaga cccgcctgga acgcatcgag
     2821 gataaggaac tcgaagtgtt tgcggtcccg gtgcaccggc acgccccact cgcggtcgtg
     2881 atagcggcgc agtaacggac tgccctccgc ccattcgcac cggctgacgg ctgcgctgcg
     2941 cgtgctcacc tcgccggcgc cccgacgggg ttgcgcagca cgccgatccc ctcgatctcg
     3001 gcctcgaggg tgtctccgac cttgagccac ttcggcgggg tgaatcccat gccgacgccc
     3061 tcgggcgtac cggtcgcgag caggtctccc gattccaggg tcagtcccgc ggacagggtc
     3121 tcaatcaact gtgcgactga aaacagcatg ctggcggtcg tcgagttctg ccggacctcc
     3181 ccgttgacgc gcatcgacag ccgcagtttc tgcggatcgg gaatcgccga gcggagcacg
     3241 atccacggtc ccatcggggc gaacgtgtcg agcgacgtcc ccttgaacca ctgttgatgc
     3301 cggcgctgca ggtcgcgcgc ggtgacgtca ttcatgatcg tgtatccgaa cacgtagtcc
     3361 agcgctttgg ccgcggggat gtcccgtccc ctgcgtccga tgacggccac gagttcgact
     3421 tcatagtcga gcgactgggt gacgcggtgg tggatcacgg gcatctcatg gccgatgact
     3481 gtcgttggtg acttcgtgaa gaagacgggc acctcgggcg gcgccacacc gccttcacgc
     3541 gcatgctcgg cgtagttgcg acccatgcag aagatgttct tgctggggcg cgggatcgga
     3601 gccaggagct tcgccttcga cagaggagaa ctctcccgcc ccgctctcac ccatgcgtcc
     3661 gcttcacgtc ccagcgcccg cagcgccagt gtgggagttt cttcaatcag cgtgatcatg
     3721 tccttcggca cgctgagctt cttgcggcga gcgagggcct gcagatccac gatccgatcg
     3781 ttgacaacga aacccaggcg cgattctccg gcgccctggt acgtggcgat acgcatttcg
     3841 acctcccgct gttcggcagg tgagtttcgg caggcacaga aggctaccct ccgacaaccc
     3901 ccgatcgtta gcgactcccg aatattgccg cgaagcggcc tgcggcgctg ttgaggaggc
     3961 cgagcctgag gcgaggccgg cggattctgc acgttgcttg ggtctggagc cgagctgcag
     4021 ggaggccggg agaatttcgc tcgttgcgac taggtggagg ttagatatgg cgaaagtgac
     4081 ggtacttgcg gggactacga atggtatgta cgcgttcgag agcgacgccc ggcgcgctaa
     4141 gtggaagaaa cgcggcccgt atctcaaggg tgtatcggtc aatcatttcg cgtacgagcc
     4201 gaagtctaag acactatacg ctgccaccct cgatcaagga gtgatgacga gcaagacgct
     4261 cggccggtcc tggagtgcgt tgagcgatgg gctgccgatt cggaaggtgt ggtcggtggg
     4321 agtgaatccc aaggactcca gcgagctgtg ggccggcact cacttcagtt atctatttag
     4381 aagtaaggat cgcgggcgga catggtcact gctcgagggc tacctgaagg caccaggaaa
     4441 ggaggaccgc tggggagact gggcatttgg aacgattgga aactccttgc acgggatcca
     4501 tttcgatccc gctgatccca agcggatgat agtggtcagc agcaccaacc atggcgctgt
     4561 tcgcaccgcc gacggcggcg agacgtggga gtacgcgcgc aacggcgtct ttgaatcatg
     4621 cccctccgct caccagtccg ccctcggcaa gccaccggat gagcaggcgc agagcgttaa
     4681 agagcatctg gagcaggtcc actcctgcac tcatcgcatc agcattgtgc cggccgcgcc
     4741 caatgtcgtc taccggcagc aacactgcgg ggtgtatcgc agcgacgatt ttggttcgac
     4801 gtggcgcgac atctcgagcg ggctccccga aaggcacggc ttcccgctgg cggttcatcc
     4861 caaggatacg caaaccgcat tcgtggtgcc agcctaccag gggaagtgcg acaagcacaa
     4921 cagctgcatc atcggtgcgc tggatgtcta ccgcacccgg accggggggc gttcatggga
     4981 gaagctgagc gagggtctcc ctcgcgacgt gcattgtgtc gtcctgcgcc acggcatgga
     5041 catcgaccgg ctgcaaacgc cgggggtcta cttcggtacc accaccggag acgtgtatgc
     5101 gagtcctgac agcggcgatt cctgggtgag gctggcgaca agactaccgc gcgtccaggg
     5161 gattaccgcg atcgtcgcct gacgtctgag atcagggcac cgagacagac gcggtgaccg
     5221 gcgcccgctc ccacacttcg aacgagtagt cgtagtcgtt gtctgcatcc ccggcgtgct
     5281 tctcgcgtga gatgagcgtc cagtcgctga agtcgacgcc cggaaagatc gcatccccag
     5341 tgacggttgc gtgcacgcgg gtgaggtaga tgtgatccgc gtactcgagc gccgagcgat
     5401 aaacctccgc acctccaatg acgaacactt cctcctcgcc gttgacgcga gcgatggcca
     5461 cggacagggt ctggacaacc tcgcaacccg gcgcggtgaa gtcaggattt cgcgagagca
     5521 cgatcgtccg ccggccggga agcggccttc ccagacgcca cacgatcgct tcatgtgtct
     5581 tccgaccgac gatcacggta tgtccggtcg tcattcgctt aaatctcttg aggtcggttg
     5641 cctggtgcca cggaagatcg ctgttcctcc cgatcacgtt gttctcggac atggcgacag
     5701 taattgagat tttcggcgac atgggggtcc tcgctcccta caccggtgtc ggtatctttc
     5761 ttcgagacag ccgtgggtag tagtccgtca cggcaaaatg gtgatgccgg aatgcaaaga
     5821 tgtctgtgat gtccggatcg acgttcaccg tggggaatcg ctgcggctcg gtcgccagca
     5881 tcacctgcac gtcttcaatt tgattcaggt aaatgtgcgc gtcgtcgatc cagtagacga
     5941 gctccttcgg tcggtacccg gtcacctgtc cgatcatcag cgccaacgcc gcataatgaa
     6001 tgaaattaaa tgcgagcccg accggaacat cggcgctgcg ctgccggtgg gtgagggtca
     6061 gctcgcgcgt ttccgtattc acgtggacgt gcacccagcc gtggcagggg ggaacgacga
     6121 cgtgtcggcg cgcgccgttc gcggcgagca cgaagttcgg aatccatggc gagagctcgt
     6181 gatgccgcgc agcgggccgc gtcctgatct gatcgatcaa atgcttcact tgatcaaacg
     6241 ggtcgccgcc ggccgttgga aaccggcgga acgcggcgcc atatgatcct ggtcccaggt
     6301 ctccggcagc gagaccgaac tcctcgcagt gctcagcggt cacccaccgg ttccaccaga
     6361 agcagccgaa ctgttcgagt tcctgctggg tctgggcgcc gttaatgaag gcgcagatct
     6421 caccaatcgc ctgatgaaac ggtgacggtc gtcttccgtt ctgcggccgg ctgaggacat
     6481 cacgctcggt gatgatgggg aacccgttgg cgaagggaaa ccgcaaagcg tggccgataa
     6541 ttttcagcgc gggcaccccg tgctcggtcg ggacctcctc tccacccgtg aggatcctca
     6601 ggagcagatc gcggtactgg cggtcgggga cgcgctgttc aaaagggcga tacatgaatt
     6661 gaccccaggg gggaggtgcg ggcgaaggag gagtggtgtc cgtctgatgc ttcgtgggga
     6721 gcctgtcgtg gaggcgtcca agagcagact cgtaggctcc tcccccctgc aggtctccgg
     6781 ctatattcag ggccagttcg gaaagtcctg cccggcggac aacaagatgt tgtggataag
     6841 ctgttgatta tccaccaggg ccaggggggc gagcaaatgt ttggatcggc cagggaagca
     6901 actccctcct gtcaaaggtg aacggcgtga aggactttga gatgtcggtt cgcggccgcc
     6961 tcgccgcggg gaagcggcgc gaggcgccgg actccccggg gcggcgggcc gcggtgttgg
     7021 tcccgctgta cgatacggaa gaaggcccct acgtactctt cacgaagcgc acggacgccg
     7081 tcgagcatca caaaggacag atctcatttc cgggaggagc ggccgatccc gacgatcccg
     7141 acgcgttgtc gaccgcgctc agagaaaccc atgaggaatt ggggatcgca ccgcatgacg
     7201 tcaaggtgct cggcgcgctc gatgacgtgc cgacggttgt cagcggcttc gtgatcactc
     7261 cgttcgtcgg catcatcccg cacccgtacc cgctccgcgt caatcctgtt gagatcgcag
     7321 aagtgttgtt cgtgccgctg agcatcttca gggacgcgga taaaatgcga gtcgaggagc
     7381 gcgaccggga gggcgaacgt gtcaccgttt acttctatta ttacggcccg cacgagatct
     7441 ggggcgccac tgcccgtatc atgaagggct ttattgacac ggtctttgga gacagcgcat
     7501 gagatccccg gaggatcacg gaagcggcat tctgacccgc ttgggccatc attttttgac
     7561 ccccgaagcc aataatcgtc caaaattgcc gaattgtgtc tttttctcac tcgagctcga
     7621 attatctcga tatttcatgg ctatccgcca tatatacggg atatgttcgc ctgttccggg
     7681 gtaggactct accttgatat acttgaaggg ctacatgcag gaggcgtcca tgaagcagct
     7741 tacgcagcct ctcttcaccg ttagcgtcgc gagcagcctc gttggtgttt cctcacagac
     7801 attgaggaag tgggaaacgg caggcctggt cgcaccagtc cgcgaccgaa gcacgcggag
     7861 acgtttgtat tcgtgggcag agattgagcg catgcagcag atccgttacc ttctgatgcg
     7921 caggaggatt ccgcttcgag aagtgaagcc aatgcttcgc gcgctggccg ctcgtcggcg
     7981 acctgggatc gtgcccgacg atcgccgcaa gcgcgcaccg ctgtccgtcc cgatcgcgct
     8041 cccgcgctga gtacctggtg acggcactca aggccctctc gaattttcga gagggccttt
     8101 gagtttcggt atacttgtct catgacccgc gtgtacgctg accacggcgc cacaacccca
     8161 accgatcctc gcgtggtgga ggcgatgctg ccgttcttct cggaaaagta tgggaacgcc
     8221 agcagcgtcc atcaatgggg acaggatgcg cgcgaggccg tggatgctgc gcgcgaactg
     8281 atcgcggccg gaatcggggc aaagccggcg gagatcgtgt tcaccagcgg ggcgaccgag
     8341 tcggacaact ttgcggtgtt cggcgccgcg tatgcgaatg agaagaaggg ccgtcatgtc
     8401 gtcacctctt ctgttgagca ccatgccctg ctggagagct gccgttttct agagaatcgg
     8461 gggtgggagg tgacgtatct gcccgtagac cgccctgggg tggtcgaccc tgacgacgtg
     8521 cgcaggtcga tgcgcgacga cacggtgctg gtttccatca tgcacgccaa caacgagatc
     8581 ggaacgacgg agcccattgc agagattggg cgaatcgtca aggagcgcgg cgccttgatg
     8641 cacactgatg cggcgcaaac gttcggcatc gtgcctgttg atgtcgacga cctgtgcgtc
     8701 gatctcctgt ccatatcggc ccacaagcgc tatggaccga aggggatcgg cgggctctat
     8761 atccgcaagg gcgctcacgt cagccgcatt cagcacggcg gctcgcacga gcgcaaccgc
     8821 cgcggcggaa cccagaacgt tcctgccatc gtggggtttg ggaaggcgat ggagatcgcc
     8881 ctcgcgtcga tgggcgagga gagcgctagg ctggcgcaga tgcgagatgg gttgacggac
     8941 ggcctcgctc ggatcgacgg ggcgcatctg aatgggcatc catcccggcg cctcccaggg
     9001 aatgtgaact tctccttcga aggcgtcgac agcgagtcgg tgctcatggc gctggacttt
     9061 cagggtgtgg ctgcgagcag cggttctgcc tgcacctctg ggagcctcga gccatcccac
     9121 gtcctcgcgg ccatcggcag gcctcctcac attgccgcag gcacgatcag gctctctttg
     9181 gggcggccga ccacgcctga cgacgtggcg tacctgtcgc gcgtcgtgcc tgatgtgatc
     9241 agcagcctgc gtggcgcgtc gcgaaaggtc tcgcgggcga gccgatagcc aggacgcggc
     9301 ctcgccgcgc cgaattgcaa aaactgacgg actgatccga acccatcggc atgaccggtt
     9361 cctacgttcc cattcttgtt cactttgcgc tcgtcgtcgc gctcaccgcg ctgctgctct
     9421 ccgcgcacgc gctcctcggc cgcggccgcc cgcttcccgg taagtacgag gcgtatgagt
     9481 ccggcgtctg gccgatcggc tcggcccgag aacgcgtgcc catccgctac tacctgatcg
     9541 cgatgttgtt cctgctcttc gatattgaag cggtgtttct ctacccgtgg gccgtcgtcg
     9601 cccgcgagct gcggatcttc gggctcatcg aaatcatcgt cttcatcggt gtgctgggac
     9661 ttggtctcgt gtatgcctgg aaaaggggcg cgctggagtg gcagtaagac ccgaaccacg
     9721 agggacgacg atcagcaggg cgccgtcatc ccgcgccgtc accgacgccg acatccgcgc
     9781 gctcgaaggc aacatcctca cgagcacggt cgaccggttc gtggcctggt cgcggaaaaa
     9841 ctcgatctgg ccggtccagt tcggcctcgc ctgctgcgcc attgaaatga tgtccgcggc
     9901 ggcgtcccgc tttgacatcg cccgtttcgg cagtgagctg tttcgcgcat ccccgaggca
     9961 ggccgacctc atgatcgtct ccggtcgcgt cagccagaag atggcgccgg tcgtccagca
    10021 catctacgag cagatgctgg atcccaagtg ggtcattgcc atgggcgatt gcgcgagctg
    10081 cgggggcgtg ttcaacaact atgcgttggt gcagggggtg gacaagctca ttccggttga
    10141 tgtctacgtc gccggctgcc cgccgcgtcc cgaggcgctc ctatatgggt ttctcaagct
    10201 ccaggaactc atcagcgcac gatcatgacg cgcgacccca ttgacatgct cacgcaacag
    10261 ctcggagcga ccgtgctcga gcagacccgg ttccgggatg acctcactct cgtgctctcg
    10321 acggatggcc tgcgtgccgc gctgcagtcc gcgcgagccg cagggttctc gctgctcact
    10381 gacctgaccg cagtcgaccg gtatccggcc acgccgcggt ttgaggtcgt gtatcatctg
    10441 accgcgccgg atgcgcgggc gaagatccgg ttgaaagctc gcatcgcgga gaccggcgcc
    10501 gtgccgtcgg caacgcccgt gtggccggga gcgaactggc tcgagcgtga ggtcttcgac
    10561 ctgtacggca tccgtttcga cggtcatccg gacctgaagc gaatccttat gccggacgac
    10621 tgggaagggt atccgctgcg caaggatttt ccgctgaccg aagagccggt gcaatttttt
    10681 gggcataccc ccaaagtccc cagcgagatc attccgaaga gtccgccgaa aacataaacg
    10741 tgaatgagct aacacgcgag accctggttc tcaacatggg tccgcagcac cctgcgaccc
    10801 acggagtcct gcgcctcctg ctcgagctcg agggcgaagt catcctttct accgagccgg
    10861 ttatcgggta cctgcacaca ggcttcgaga aagagatgga gacccggacc tatcatcaga
    10921 acatcgtctt tcccccgcgg atcgagtacc tcgcgacgtt catcgaagag caggcgtacg
    10981 tccttgccgt tgaaaagttg ctggggatcc gggcgccgaa acgcgcggag acgatccgcc
    11041 tgatgcttgc agagctctcc cgcatcgcca gccatctggt ctggctgggc acttctgcca
    11101 tcgacctcaa cgtctccagt gtcttcatgt actgcttccg ggacagggag cagattctcg
    11161 atatgttcga gatggtcagt ggacagcgca tgatgcacgg atacttccgg atcggcgggc
    11221 ttcaatggga ccttccggcc ggattcaccg acagcgttcg caccttcctg gaaggctttc
    11281 ctgggcgcat cgacgagttc gagacgctgc tgaccgacaa cctgatctgg cggcagcgca
    11341 ccgaaggcgt gggcgtcctg agcgcggagg atgcgcttcg ctacgggtgc acaggaccga
    11401 tgctccgcgg ctccggcgtg aactacgacg tgcgcaaggc atttccgtac ggtggctacg
    11461 acgaatacga gttcgacgtc ccgttggggg agcacggtga tagcttcgac cgctacaaga
    11521 tccgggtcga agagatgcgg cagtcgcgcc ggatcatcct gcaggcgctg aaccggcttg
    11581 cggacggacc gattgcaatc gacgatcgga agatctcgct gccgccccga ccggagctcg
    11641 tccgcagcat ggaggctgtg atccatcagt tcaagctcgt cagtgaggga atcaatccac
    11701 ctgtcggcga ggtctacgcg gcggtcgaat cgccgcgtgg agagaagggc tactatatcg
    11761 tcagcgacgg aagcaaccgc cccacgcgag tccgcgtgcg cgcgccatcg ttctcgaacc
    11821 tgcaggcgct tccggtgatg gtctaccggc ggaccatcgc ggatgtcgtc gtcgtgattg
    11881 cctcgatcga catcgtcctg ggagacgttg acagatgaat gccgggaacg gggagcgagg
    11941 aacagggaac gggatttcgg aatcggcgaa ggtcgagatc cgccgcctca tcgcgcagtt
    12001 cccccagcga cagtccgcgc tgctcggggc gctgtttgtt gcgcaggatg aggttggaca
    12061 cctgagtccc gaggcaatcc gggacgttgc cgaggcgatg gagctggccg aaagtgaagt
    12121 gacctcggtg gcctcgttct accatttata tcagttcaag ccggcggggc ggcatctcat
    12181 ccaggtctgc acgaaccttt cctgcatgct gatgggatgt ggccgcattc tggagcatct
    12241 gaaggaccgg ttgaggattg atgtcggtca gacgacgccc gacggccgtt tcacgctgaa
    12301 gacggcggaa tgcctcgcgg cgtgcgaact cgcgcccatg atgcacatcg gtgacgaccg
    12361 attcggtccg ttgacgccgg agaaagtcga cgaaatcctt ggtcgtgttg tttcccggac
    12421 gtcgaacgga tgagcgaacc cttccttctc aaatacatca atgaacccga tcagggggac
    12481 ctcgaggcgt atgtgtcccg cgggggttat gagggcatgc gccgcggcct gtcgatgacg
    12541 cccgaagcgg tcatcgatga ggtggacaag tcggggctgc gcggccgggg cggcgccggt
    12601 ttccccaccg gccgcaaatg gaagttcatt cctcgggatc ccgccgtcac gaaatatctt
    12661 gtcgtgaatg ccgacgaggg cgagcccggc acgtacaaag accgcaccct gatggagggc
    12721 gatcctcacc gcttgatcga gggcatcatc attggaagtt ttgcgatcgg cgcacggcag
    12781 gcattcatct atctccgccg ggagttcgcg cctgcacggg cggcactgac gaaagcgatc
    12841 gcacaggcca gggccgggaa ctacgtgggc cgcaacatcc tgcacaccgg ttacgatctc
    12901 gaaattattc tcgcgacagg cgccggcgca tatattgcgg gagaggagac ggccctgttg
    12961 gagtccctgg agggccgccg cgccatgccc cgtctcaagc cgccgttcta tcccgccgtc
    13021 aaaggcctct acatgcagcc gactgccgtc aacaatgtcg agactcttca ccacgtggcg
    13081 tggatcctgg cgaagggcca cgagtggtac aaggcgcagg gctccccgat cctcctctcg
    13141 gtaagcggcc acgtcgagcg ccccggagtg tacgaagtcc cactggggac cacaatcaga
    13201 tccaccattg atctggcggg cggcatgcgc cgcggtcgga agttcaaagc ctgctttcct
    13261 ggcggatcgt cgtcggcgat cctcacgggc gcctatctcg acacgccgct ggactacgac
    13321 gcgctcccca aggtcgacgt acccggcacg atgcttggat ccgcggcggt gatcgttatg
    13381 gacgagacgg catgcatggt ggaggtcatc gcccgcacgg tggagttcta tcgcgacgag
    13441 tcgtgcggca agtgcacgcc atgccgagag gggaccgtct ggctgggtca ggtcttccac
    13501 cggattctgc acggccaggg gcgtccagag gacctcgatc ttctcggcgg gatcgccaag
    13561 gggatgacgg ggacgtgctt ttgccccctg ggggagagcg tgcctcccag cctgaatgcc
    13621 tcgctgagat tcttccggca tgagtatgac catcacatca agacaggcac gtgtgacgtc
    13681 aaatttcaca gagcgagtcc gtgagcgatc aacccagggt cacgttggcc atcgacggca
    13741 cgccgatcac cgtcccgaaa ggcacgacgg tctaccaggc ggcgaagcaa tcggggatcg
    13801 agatccccat attctgctac catgaccgga tgcccccgct tggcgcgtgc cggatgtgtc
    13861 tcgtcaaagt cgagaagatg gcgaagctgc aaacctcctg cacgctcgaa gccctcgaag
    13921 gcatggtcgt cgacactgcg acggcggacg tgaaggcggg gcaggaagcc atcctggagt
    13981 ttctcctgat caaccatccg ctcgattgcc ccatctgcga caaaggcggg gagtgcccgc
    14041 tgcaagatca gactttcaaa ttcggacccg gacggagccg gtttgtcgaa gccaagcgcg
    14101 atttcgccaa acccgtgtcg ctgggtccgg tgctcgtgct ggatcgcgag cggtgcattc
    14161 tgtgttggcg ctgtgtgcgc tttggcgaga tcgtcgcggg tgacgacgcg ctcaaagggt
    14221 tcgagcgcgg gttccacagc gagatcaata cgccgttcac attgcccgtg cggtcaaaat
    14281 tcatcggcaa caccatcgag atctgtccgg tgggcgcgct gacggcgaag tcgtaccggt
    14341 ttatcgcgcg cccctgggat aacacgccgg tcacgagcac ctgcacgctg tgcggcgtcg
    14401 gatgtgcggt ggagttcgac gtccggggag gaaagatcgc ccgcacgcgg ccatctgagg
    14461 atccggccgt aaacgacatc tggctgtgcg acctcggatt ctttggccac acattcgtgc
    14521 accacgaggg ccggctgcgg cggccgctga tcaagcggga aggcgggttc gtcgaggcca
    14581 cgtgggacga cgcgcttgcg ctggtcgcct cccgcctcga gcgcgccggc gcgcgcgtcg
    14641 cgatgctagg gggcgcgagg ctgacgaatg aagacgcgta cgtcgccacg cggttcttcc
    14701 gcagcgtcgt gcggaccgcc catatggatc accgcgtaga cgcgcttgca cggggtccag
    14761 acctcagcat gtcctggggg atgacgacgg caatcgagga gctcgcgcgc gccgattgcc
    14821 ttttgctggt gggctgcgac atcaccgaaa cgtaccccgt gatctggctc cgcatgaagc
    14881 aggccgtcga acacggcgcg acgctcatca ccgtcggcac gcgggcgctc gagatcgaag
    14941 cctctgtccg cctgccgttt ctgcaccgcc aggctcacgg cgccgagatc gtgcgcgccc
    15001 tcggcgaatc cgtgcgaggt tcccctccgg gcgggccggt ggaggggatt gatcgcgatg
    15061 ccatcgccgc tgcggcagcc gcgctcagcg ccgcgtcgca accagtggta ctggtcggcc
    15121 gcgaggccat ggacgcgccc gacggctggg gcatcctgct cgcgacggga gaaatctgcc
    15181 gcaagatcgg tgttgacctc aatgtgatgc gcggaaaggg taacgcctat ggtgcgtcgc
    15241 ttgccgggat cctcccggat gccggtccgg gtggccgtgc actggaagag gccgcagccg
    15301 acttgaaggc cctctggggc gaggtgaata cggacaaggg gatgaccgcg ccggagatca
    15361 tcgaggccgc ggcggccgga gggctcgata tgctgtatgt cgcgggcgcc gatccggcca
    15421 acgatgtccc ggaccgggag cggtggcgtg cagcccgtgc gcgggtaccc tttgtcgtcg
    15481 tacaggacgc cttcttgagt gaaacggctc agtcggccga cgtcgtgctt ccaactctcg
    15541 tcgccccgga aaagaacggc accgtgacga gcatcgaggg gcgcatccgg cgcctacgcg
    15601 ccgccgccca tgggcccggc gaggctcggg gcgattggac gatctttgcg gcgctggctg
    15661 tgaaacttgg gaagacgatt ggctacagca gctgggaaga aattttcgac gagatgcggg
    15721 gactgatccc ggccctggcc tttgacgcgc gcctgcccat gcgccaccct ccgatcgacg
    15781 ccggtccaga cgagggccgt cacgccatcg aagaggcaaa gggctatccg tatgcactca
    15841 tcctgggcga cgtgctcttt gatcggggtg ctttgatgac caggtctccc acgatcgcgg
    15901 atctcgcggg tgagccatgg gccttgattc acccgaacga cgcggcggcg ctcggcgcgg
    15961 tccaagacgc atcggtcgtg atttcctccc gtcgaggctc gatcgcggtc cgtgccagga
    16021 tctcttcgtc gatccgccct ggggaggtct ttcttccgcg cgggtacgat gctgcgcctg
    16081 ccaatagact cgtcgatgcc cgcgaaatgc tgacctacgt ccaggtccgc gcgctcgttc
    16141 cggcgaccac gtgagctcgg tcgccgccgc cgcgatcaaa agcggggtgc tgctcttcct
    16201 gctgctcacg gcatgcgcgt acatgacgct tctcgagcgc cggctcctcg gcaagttcca
    16261 gattcgatac ggcccgaatc gcgtcggccc attcggactc ctgcaaccac tggccgacgg
    16321 catcaaactc atcttcaaag aagactttat gccggcccgc gtggatgtgt tggtgtactg
    16381 gctggcgcca gcgatttcga tggtcaccgc gctcttcgtc tatgcggtca tcccgttcgg
    16441 tccgc
//
LOCUS       DBNGSB010000119         3684 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2229769, whole genome shotgun sequence.
ACCESSION   DBNGSB010000119 DBNGSB010000000
VERSION     DBNGSB010000119.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2229769"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..646)
                     /locus_tag="AC159B_03300"
     CDS             complement(86..646)
                     /locus_tag="AC159B_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376809.1"
                     /translation="MVPESIHQKLGFARARLANLEALIADNRLAADPDARHQLTQEFF
                     FHVVGATEYLAQLVNERRSLQLRSEDVAVHKVAKELAKRDPLDPLLVALKGLSADTRT
                     TGLPADPYSEEGLIYRTINYRNEVVHRSTNPFHFVLSAGPKIAFFWLDPRDPSRGQST
                     RPVDVDLTSMFSVVDQRCRSVLAILG"
     gene            complement(689..1294)
                     /locus_tag="AC159B_03305"
     CDS             complement(689..1294)
                     /locus_tag="AC159B_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376810.1"
                     /translation="MEPMSQYRWEVIQVLSGNGPVDAATLIGSNPDKGSIPLETDDWD
                     GAADPLFTSALQVVREVLDSNREFDWSLKPLFRLEMAYLLLWSAIERFASFSYHLGGE
                     PWQKVKQLAAEPAFAEALRREVRENRRRYRADRAGVDVTLSPTNPEKSVAYYYQIRSN
                     ITHRGKGVVRDHEILWASLSELLCIFRAVLDAAFAQRMRPL"
     gene            1602..2387
                     /locus_tag="AC159B_03310"
     CDS             1602..2387
                     /locus_tag="AC159B_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HIR3376811.1"
                     /translation="MMTDWYQKSINLLALNGKGERTQEAYTRAVRMLSEFYSKSPDEI
                     SERELEDYFLHRKNVDHWSANTMRICYCGIRFFFVHVLQRNWHLLDILRAKSEARLPA
                     VLSREEVRALLGRVRTAHNYAFLSTVYACGLRLQEALHLEVSDIDSQRMMIHVHRGKG
                     AKDRFVPLPHATLKILRAHWRAHRNPRLIFPACGRDGGSAGAATTPMAKSSVQGAFRH
                     AKHAAGIRKRPVSIHTLRHYLPFLTMSCHSGTAKFSRQMREAG"
     gene            2575..3669
                     /locus_tag="AC159B_03315"
     CDS             2575..3669
                     /locus_tag="AC159B_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HIR3376812.1"
                     /translation="MLRVLADGLGKKGWDCAASPPIIQRGIYDIALDTSGFAEGMLLR
                     SDISLTSCAARAWFPPRRHRLTPAEHWAQAYEQYLRDVRALARATIVNYVPFIRDFLK
                     DRFGDGRVTLSRLCAGDVVRFVQRQVSHLHRKRAMLMTTALRSFLHYVRYRGDITPDL
                     AAAVPVVANWSMTSIPRAIAADQVRQLLTSIDRRTAIGRRDYAVLLLLARLGLRSGEV
                     AFLNLDDIDWNAGLLSVHGKGGQRSELPLPAEVGAAIAAYLRRGRAHSTSRRVFLRAK
                     APSLGFRGASGVGSIVRQSLQRAGIDAPTMGAHQFRHGLATEMLRWGASLSEIGELLG
                     HRHPQSTKIYTKVDLKALRALALPWPGGAR"
BASE COUNT          728 a         1112 c         1084 g          760 t
ORIGIN      
        1 gaccacttcg ggtcgtctca atccttacgg gttagccaag tctcgttggc ggcctccgat
       61 caatacctgc ccagaggaca ttgagctacc caaggatcgc cagaacgctt cgacaccgtt
      121 ggtcgaccac cgagaacatg ctcgtgaggt ccacatccac ggggcgagtg gactgcccgc
      181 ggcttgggtc ccgggggtcg agccaaaaga acgcgatctt cggacccgcg ctcagtacga
      241 agtgaaaggg attagtgctg cgatggacga cctcgtttct gtagttgatg gtacggtaga
      301 ttaatccctc ctccgagtac gggtccgccg gaaggcccgt cgttcgagta tcagcagaga
      361 ggcctttgag cgcaaccaag agcggatcca acggatctcg tttcgcgagc tccttggcaa
      421 ctttgtggac ggcgacgtcc tcggaccgca actgaagaga gcgacgctcg ttgacgagct
      481 gcgcaaggta ctcggttgcg ccaactacgt gaaagaagaa ttcctgggta agctggtggc
      541 gggcatccgg gtcggctgca agtcgattgt ccgcgatcaa cgcctccagg ttagcgaggc
      601 gcgctcgtgc gaacccgagc ttctgatgaa tgctttccgg caccatttac ttggggttca
      661 cggtaggaga cgttgcctgg ctaatcactc acagcggccg catgcgttga gcaaaagctg
      721 catcgagaac cgcacgaaat atgcacagca attcgctaag cgaggcccaa aggatctcgt
      781 ggtcgcggac tacgccctta ccccgatggg taatgttgct tcttatttga tagtagtacg
      841 ctacactctt ttcggggttg gtcggcgaca gagtgacgtc cactcctgct ctgtcagctc
      901 tatagcgccg gcgattctct cgaacctctc gtcgcaacgc ctcggcgaac gccggttctg
      961 cggcgagctg cttcactttc tgccatggct caccacctag gtggtagctg aacgaggcaa
     1021 aacgctcaat agccgaccag agtagaagat aggccatctc tagacgaaag agcggcttca
     1081 agctccaatc gaattcacga ttgctatcca atacctcgcg cacgacttga agagccgaag
     1141 taaagagagg atcggctgcg ccatcccaat cgtctgtttc gagggggatg cttcctttat
     1201 ccgggttgct gccgatgagc gtcgctgcgt cgaccgggcc gttgcccgaa agtacttgaa
     1261 taacttccca gcggtattga gacatcggct ccaactggac gtacgaccca gaggaggctc
     1321 atctttggac ggctaacgac aaagctaagc cgcgagctgg acgggcgcga ctcttaccgg
     1381 cgcctcgccg agcaagggtc gtgaccggcc ggatcgtcag cttcagcgtc ttgttcaagt
     1441 aagccgcttc gcggcagtgt ttcttcacca gagtagtccc atcgtctgcg ctctgatcgc
     1501 gcgaccgaga gtctagtcgc gtcagtccat ctctgttctc ctctttcggc ctgccgtggc
     1561 gagcggcggg catttctaca tcccgactga ggagaatttc catgatgaca gactggtatc
     1621 agaaatccat caacctgctc gcgctcaacg gcaagggcga gcgcacccag gaagcctaca
     1681 cccgggcggt gcgcatgttg agcgagttct atagcaagag cccggacgag atcagcgagc
     1741 gcgagctcga ggattacttc ctgcaccgca agaacgtcga tcactggtcg gcgaacacca
     1801 tgcgcatctg ctactgcgga atccgcttct tcttcgttca tgtcctgcag cgcaactggc
     1861 atctgctcga catcctgcgc gcgaagagcg aagcgcgcct gcccgcggtc ttgagtcgcg
     1921 aagaagtacg cgcgctgctc gggcgcgtgc gcaccgctca taactacgct ttcttgagca
     1981 cggtctacgc ctgcggcctg cgcctgcaag aagcgctgca tctggaagtc agcgatatcg
     2041 acagtcagcg aatgatgatt cacgtgcatc gcggtaaggg cgccaaagac cgcttcgtgc
     2101 cgctgccgca cgcgacgctc aagatcctgc gcgcccattg gcgagcacac cgcaacccgc
     2161 gactcatctt ccccgcctgc gggcgcgatg gcggctcggc gggggccgct accacaccca
     2221 tggccaagag cagcgttcaa ggggctttcc gccacgccaa gcatgcagcc ggaatccgca
     2281 aacgcccagt ctccattcac accctgcgcc actatctacc cttcctgact atgtcttgtc
     2341 actctgggac agccaagttt tcccggcaaa tgcgtgaagc cggatagaga tagtctgggc
     2401 tctttccatc agcccctgag taggggaagg agtccaccat gaagtgcatc atcaataacc
     2461 aagtggttct gtcgcgagcg ccggaagaac caattgcggc ctacctcggc ccgtttgcag
     2521 agtcggtgag tgcacagggt tatgccctgg attcgattca ccggcaggtt ttgattgctg
     2581 cgtgttttag ccgatggctt aggcaagaag gggtgggact gcgcagcatc acctccgatc
     2641 attcagcgcg gtatttacga tatcgcgctc gacacgtccg gctttgccga ggggatgctg
     2701 ctgcgctcgg acatctcatt gacttcctgc gccgccaggg cctggttccc tccgagaaga
     2761 caccgactga ccccggctga gcactgggca caagcatacg agcagtactt gcgcgacgtg
     2821 cgcgcgttgg ccagagcaac gatcgtcaac tacgtgccgt tcatccgcga ttttctcaaa
     2881 gaccgtttcg gcgatggacg agtcacgctc tcgcggttgt gtgccggcga tgtcgtgaga
     2941 tttgtacagc gccaagtgtc gcatctgcat cggaagcggg cgatgctcat gaccactgcg
     3001 ctgcgctcct tcctccacta cgtacgctat cgcggcgaca ttacgccgga tctggccgcc
     3061 gccgttccag tcgtggccaa ctggtcgatg acgtcgatcc cccgcgcgat tgcggcggat
     3121 caagtccgtc agttgctgac cagtattgat cggcgcactg cgatagggcg tcgcgactac
     3181 gccgtcttgc tcctgctcgc gcgactgggt ttgcgatccg gtgaagtggc atttctcaac
     3241 ctcgatgata ttgactggaa cgcgggccta ttgagcgtgc acggcaaggg tggccagcgc
     3301 agcgagctac ccttacccgc ggaggtcggc gcagcgatcg ccgcctatct gcggcgtggg
     3361 cgagcgcaca gcaccagtcg gcgcgtattt ttacgcgcca aagcccccag cctcggtttt
     3421 cgaggggcga gtggcgttgg ctcaatcgtt cggcagtcgc tccaacgcgc cggcattgat
     3481 gcccctacta tgggagcaca tcagtttcgc catggattgg ccaccgagat gttgcgctgg
     3541 ggcgcatcgc tcagcgagat cggcgaattg ttgggtcatc gccatcccca gagcacgaag
     3601 atctacacca aggtcgatct caaggcattg cgtgcgctgg ctttgccgtg gccgggaggt
     3661 gcgcgatgaa tacgcttcga cagg
//
LOCUS       DBNGSB010000120         4467 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2383102, whole genome shotgun sequence.
ACCESSION   DBNGSB010000120 DBNGSB010000000
VERSION     DBNGSB010000120.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4467)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2383102"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="AC159B_03320"
     CDS             complement(<1..239)
                     /locus_tag="AC159B_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376813.1"
                     /translation="MQRLKSTALLLFLLSVPGTVSAQAGITSPRSQFGHDIGDDYFLV
                     NYTQMIEYWQKLDRESDRMKMVRIGTTAEGRPMWMA"
     gene            complement(310..1215)
                     /locus_tag="AC159B_03325"
     CDS             complement(310..1215)
                     /locus_tag="AC159B_03325"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3376814.1"
                     /translation="MSGKPYWVLALSLLGIAFAGPLVRLSRADPVAIAVWRLGFSLLI
                     VGAFLMATREWRDWQRIAPRELLLALMGGISLALHFWAWNASIQLTTIAASVTLVSLQ
                     PAVVATISAIALREVPSGRQILGIAIAIAGAFIIAAPDLRGGIAPSGNRPLLGNLLAT
                     SAAVTAAIYYTIGRRLRGSLGIWAYVGIVYSAAFVSLLAIALSRGTPLAAQPPREIAI
                     FAGLAIGPMLIGHTGMNWALKYLPAYVVNLTVLGEPVGATLLGALIPSIRQIPTVNTL
                     VGGAIVLAGVVLAAGGPSRRSSINA"
     gene            complement(1270..1890)
                     /locus_tag="AC159B_03330"
     CDS             complement(1270..1890)
                     /locus_tag="AC159B_03330"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HIR3376815.1"
                     /translation="MKTAMRAVTHAVALGGLLVGGAVWTISQQHPLYANPGKLLRLPA
                     AVVDASQPTEQAFRIGEVLNKYTHDRARAFRIADALVDQARKENLDPVLLVGVLLTEN
                     AQLDPRARSSVSARGLMQVMPIHSGNWGCGSKDLFDVESNICHGVQILKQAMHDAPNM
                     RKALLRYNGCVRGRNTPNCHTYDDKVLRHASRAAEQMRLVKTLKTD"
     gene            complement(1979..2575)
                     /locus_tag="AC159B_03335"
     CDS             complement(1979..2575)
                     /locus_tag="AC159B_03335"
                     /inference="COORDINATES: protein motif:HMM:NF024949.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3376816.1"
                     /translation="MAEWIGRELERIRKLRGLRREDVAARLSRPVSASTIRNIEHDED
                     YNVSLDLLQQIAAVLGAKILVSLEAEDLKRWQTPPGQRPAIGVADVPTEGNERLIDNE
                     YFIRYIRARYPDCPLTNSQIGRRMWDFAKPRGAALVKGRMQVKKLPATESIATYRLPT
                     TAAQYRVHERDIEVLHGFADRLGRAYRDEKGRILVPLI"
     gene            complement(2796..3548)
                     /locus_tag="AC159B_03340"
     CDS             complement(2796..3548)
                     /locus_tag="AC159B_03340"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HIR3376817.1"
                     /translation="MADLEYEQRQSGAGDPFVHVPVRRPVKPGAPRWQRVLKAALHGL
                     GAAILLAAGTVWTLRQQNPKFLKPGQLLNLPAAVVTAKPPGTEPYELPTDEKARDEVL
                     RVRHALLRYTRDTARAQRIAGAVVAEGGKRNLSPALLIGVLLTEDVTLNPRAKSFVGA
                     RGLMQVMPMHAGKWKQCPSSDLFSIENNICYGTSILADFVKRSPNLNRALLRYNGCVR
                     GTNTPHCHTYSGKVLKFAEQAATRMLDFSMTE"
     gene            3635..4372
                     /locus_tag="AC159B_03345"
     CDS             3635..4372
                     /locus_tag="AC159B_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR3376818.1"
                     /translation="MKESEGLYRSFRFLKKALLGLAIVLIALLLFGYFFFMRHVDAPK
                     AWTAADRELQGGMLQYGESVQRRAKVFMRRPSDYYRGANGMLYATNDRLIFIGVAPGS
                     KFEDADAPPMIVSQEFPNDTLLDLRGTRLYLLTAHGVRVAHPAAARSEFAASPGETAA
                     LDSLTDYVNAIHAAQRKEAAREKRLREAVDALIKQPLYYRVKRGDAISLIAAKFDATA
                     EQIRQWNQIEGDRIKIGQRLLVKPAKK"
BASE COUNT          884 a         1348 c         1352 g          883 t
ORIGIN      
        1 gccatccaca tcggccgccc ctccgcagtc gtcccgatcc gaaccatctt catccggtcg
       61 gactcgcgat cgagcttttg ccagtactcg atcatctggg tgtagttgac gagaaaatag
      121 tcgtcgccga tgtcgtgccc gaactgcgag cgaggggatg tgattcccgc ttgtgccgag
      181 accgttccgg ggacgctgag gaggaagagt aggagcgcgg tggacttcag gcgttgcatc
      241 gattcacggt tgggatgggc tcggcgaggc gcgctctagc gggagcgggc cgccgaagaa
      301 atttaatggt caggcgttta tgctgctgcg gcgtgatggt ccaccggcgg caaggacgac
      361 accggcgaga acgatagccc caccaacgag cgtgttgact gttggaatct gtctaatgga
      421 gggaatcagc gctccaagca gcgtcgctcc gaccggctct ccgaggactg taaggtttac
      481 gacgtaggca gggaggtact tgagggccca gttcataccg gtgtgcccaa tcaacatcgg
      541 gccgatcgcc agccccgcaa agatcgcgat ctctctcggg ggctgtgcgg cgaggggggt
      601 cccacgcgaa agagcaatag caagaagact cacgaacgcg gcggaataga ctattccgac
      661 gtaggcccag atgccaaggg acccccgcag gcgtctcccg atggtgtagt agatggcggc
      721 tgtcaccgcc gccgacgtcg caagcaagtt tccgagcagc ggccgattcc cgctcggcgc
      781 gattccgccg cgaaggtcag gggcggcgat gatgaacgcg ccggcgatgg cgatggcgat
      841 gccgaggatt tggcgtcccg acggaacctc tctcagagct atcgcggata tcgtcgcgac
      901 cacggccggc tggagactaa cgagcgtgac tgacgccgcg atggtggtga gctgaatgga
      961 cgcattccac gcccagaaat ggagtgcgag ggagatccca cccattaaag ccaacagcag
     1021 ctcgcgaggc gcaatgcgct gccagtcgcg ccattcccga gtggccatga ggaacgcgcc
     1081 gacgatgaga agggagaacc caagccgcca gaccgcaatc gcgacgggat cagctctcga
     1141 gaggcggacg agcggccccg cgaacgcgat ccccaacagc gagagcgcga gcacccagta
     1201 cggctttccg gacatgacaa aaaaaaaaga gccggtgcgt tcagcaccgg ctctcttttc
     1261 cagacaacct tagtcggtct tcagggtttt gaccagtcgc atttgttcag ccgcgcgact
     1321 cgcgtgtctc aggaccttgt cgtcgtaggt gtggcagtta ggcgtgttgc ggcccctgac
     1381 gcagccgtta tagcgcagca gcgctttgcg catgttcggc gcatcgtgca tcgcctgctt
     1441 gaggatctgg acgccgtgac agatgttgct ctcgacgtcg aacaggtcct tcgagccgca
     1501 gccccagttg ccgctatgga tcggcatcac ctgcatcagg ccgcgggcgc tcacagagct
     1561 gcgcgcgcgg ggatcgagct gggcattctc cgtgagaagc acaccaacga gaagcacggg
     1621 gtcgaggttc tccttccgag cctgatcgac gagggcgtca gcgatgcgga acgcccgcgc
     1681 gcggtcgtgg gtgtatttgt tcagaacctc accgattcga aacgcctgct cggtgggctg
     1741 cgaagcgtcc acgacagccg cgggcaatct gaggagcttg cccgggttcg catacagcgg
     1801 gtgctgctgc gatatcgtcc acacggctcc gccaacgagc agcccaccca gtgcaaccgc
     1861 atgggtgacg gcgcgcatcg ccgtcttcat tggagaagac agcttcggcg gaggtgcatg
     1921 taacggcttg agtgacttct tcacaggttc tcctggaatt agtacagcgc gaacgcgctt
     1981 agatgagcgg aacaagaatc cttcccttct catcgcggta cgcccggccc aaccgatccg
     2041 caaaaccgtg caatacttca atatcccgct catgaacgcg gtactgcgct gcagtcgtcg
     2101 ggagcctgta tgtcgcgatg ctctccgtag ccggcagctt cttcacctgc atcctgccct
     2161 tcaccagcgc agccccacgg ggcttggcga agtcccacat cctgcggccg atctgtgagt
     2221 tcgtcagcgg acagtccgga taacgcgccc ggatgtaccg gatgaagtac tcattgtcta
     2281 tcaacctctc gtttccttcc gtcggaacgt cggcaactcc aattgcgggc ctttgtcccg
     2341 gtggcgtctg ccagcgtttc agatcctctg cctccagaga gaccaggatc ttcgcgccca
     2401 gcaccgccgc tatctgctgc aaaagatcga gcgagacgtt gtaatcctcg tcatgctcga
     2461 tattgcgtat ggtcgacgcc gacactggtc tggacagacg tgccgcaacg tcttcacgac
     2521 ggagacctct gagctttctg attcgctcaa gttcccgtcc aatccattct gccatttcac
     2581 tctattgtgg taatgccact gctgagatac gtattacggt acctctattg acgagtgccg
     2641 tcaacaaaaa gttatggtca gatttagaac cgcaggtagg taacaatgtc acttctgtga
     2701 cgcggctctc tgtaagctat tatcgttcaa caggttgcgc tactattgat cgaacagtat
     2761 tgcgcttcag atgtttagga acttttccgg gcgggctact ccgtcatcga aaaatcgagc
     2821 atccgggttg ccgcctgctc ggcgaacttg agaactttgc ccgaataggt gtggcaatga
     2881 ggggtgttcg tgcccctcac gcatccgtta tagcggagca gcgccctgtt gaggttgggc
     2941 gaccgtttca caaaatcggc cagaatactg gtgccgtagc aaatattgtt ttcgatggag
     3001 aagaggtcgg acgacgggca ctgcttccac ttgcccgcgt gcatgggcat cacttgcatc
     3061 aggccacgcg cgccgacgaa gctcttcgcc ctgggattca gcgtcacgtc ctccgtgagc
     3121 aaaacgccga tgaggagtgc cgggctcaga ttccgcttcc ccccttcggc gaccactgca
     3181 ccagcgattc tctgagcgcg agcagtgtcg cgagtgtacc tgaggagcgc gtgtcgaacc
     3241 cggaggacct cgtccctcgc cttctcgtcg gttggaagct cgtagggctc ggttccaggg
     3301 ggcttcgcgg ttaccaccgc ggcgggaaga ttcagcagct ggccaggttt gaggaatttt
     3361 gggttctgct gcctgagggt ccagacagtg ccggcggcca gcagaatcgc cgcaccgagt
     3421 ccgtgcagcg cagccttcaa aactctttgc cagcggggag caccaggctt caccggtctt
     3481 cgcaccggca cgtggacgaa aggatctccg gcccctgact gccgctgttc gtactcgagg
     3541 tcggccatgc cggtttcggt tgcacctcat atgccaaacc cggtatggag attgccctcc
     3601 cgcccgtgcg cgcctcccac tacactgatg ctgaatgaaa gagtctgagg gactttaccg
     3661 gtcttttcgc tttctaaaga aagcactgct cggccttgca attgtcctca tcgcactcct
     3721 gctcttcgga tatttcttct ttatgcgcca cgtcgacgcg cccaaagcgt ggaccgccgc
     3781 cgacagagag ctgcagggag gcatgctcca gtacggtgaa agcgtgcagc gcagggctaa
     3841 ggtcttcatg cggcggccga gcgattatta ccggggcgcc aacgggatgc tttacgcgac
     3901 caatgatcgc ctgattttca tcggcgtcgc gccgggcagc aagttcgagg acgctgatgc
     3961 tccgccgatg atcgtgtcgc aggaattccc caacgatacg ctcctcgacc tgcgcggcac
     4021 ccgcctttac ctgcttactg cccacggcgt gcgtgtggca catccagccg ccgcgcgcag
     4081 cgagttcgcc gccagccccg gagaaacggc ggcgctcgat tcgctgacgg actacgtcaa
     4141 cgcgattcac gccgcccagc gcaaggaagc tgcgcgcgag aagcgcctgc gcgaggcggt
     4201 cgacgctctc atcaagcagc cgctctacta tagggtgaag cgcggtgatg ctatttcact
     4261 gattgcggcc aagttcgatg ctacggcgga acagatcagg cagtggaacc agatcgaggg
     4321 cgatcgcatc aagatcgggc agcgcctgct ggtgaaaccg gccaagaagt agcacgtaag
     4381 tcgttgttga tgcgaccgca ccggagtggc attcgaatcg caatcggcat atgctacccg
     4441 tgacgcgaag tgcgagcgtc ttcaccc
//
LOCUS       DBNGSB010000121         5155 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_1158286, whole genome shotgun sequence.
ACCESSION   DBNGSB010000121 DBNGSB010000000
VERSION     DBNGSB010000121.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5155)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5155
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_1158286"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1357)
                     /locus_tag="AC159B_03350"
     CDS             complement(<1..1357)
                     /locus_tag="AC159B_03350"
                     /inference="COORDINATES: protein motif:HMM:NF024832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HIR3376819.1"
                     /translation="MRESLTARTLKGAAWIGGASIARLALRVVSIAILARLLTPHEYG
                     VVAGALIAMDFAAMIYGMGLAPTLIQRKDVRSDHVATALSSALFMALLAGAGLWVAAP
                     LVAELMRIPELTQILKVLACLTPFGAFSVLCEALLARNMQVKSVALRPLFSFTISTFL
                     VAIPMAWYGFGYWSLVAMQVADTFVTALVLGIAARGLLVRPRFSRQAFAELWPLSFGF
                     TLNQPFVYIAENTDRFLVGRLLGAGALGLYSRASFLTTTAANLFGNITRLSVFPAMTR
                     VQEDAERLRNALLKTLAVVALLTLPASAFCMTFANELVNLLLGAHWSGAVVPFAVLSG
                     ALYFRLAWRACGALFQALGRPHVNSIVHALRAATLVLGIWYAAPYGLNAICGVVLAVM
                     ASVSAAMLIAVSHAIDLSFRSIVEVHVRPAAISAVIVGTGMSFKMGFVGVPTSVALVA
                     TF"
     gene            complement(1342..2487)
                     /locus_tag="AC159B_03355"
     CDS             complement(1342..2487)
                     /locus_tag="AC159B_03355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3376820.1"
                     /translation="MILPAYLPETFGGAEQQTRRLAQALARRGAGVTLLAPRLESRTP
                     PRENEGPVSVRRFRLLAAPNLGGRHLDAFLCWCLCISSWLWRNRLSYDLIYVVHGRLH
                     AFPAVVAGKWLGKPVVVKPGRGGETHFDLSMVQRKRLVGSFFAQRIASNTTAWVANSR
                     EIAADLSRWGVPRERIHSIPNGIDIPAHGRRRSSNGVVHFLSMGRLDAEKAVDQTIRA
                     FARLPAETPAHLTILGDGPCRPELEALSRRLGQERRIVFTGAVDDVTPYLKAAHVYLS
                     TSLSEGMSNALLEAMSHGVMPLVSRVSGTDDLIEDGVSGFLFPGGDETALATRLETSL
                     AMTAERRRATGEAARETIRARFSLEKVVERHLTLYRDLIEAGPCARA"
     gene            complement(2535..3638)
                     /locus_tag="AC159B_03360"
     CDS             complement(2535..3638)
                     /locus_tag="AC159B_03360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3376821.1"
                     /translation="MITHEFPPFTGGMATYAADLAAAVSATGIEVTVLAPAYERETNE
                     TRFEVIRILRHQRLGPGSAAELLARLLATPNDAFWHGVDIRSTLILHTAHLLTGRPYG
                     ATIHGSEAAKLGGKGLRVKSARAGYRRAAVVFANSHATARVASAQISHRELRIAHLGV
                     RGEWFSAPPAACTDSRLERFLSAGPCICTVGRLEPRKGHQHVLAALRAVKATGVEIGY
                     VIAGPVIDADYRRSLERFVLKTGLRVLFAESTSNEDLRRLYATSVCHVLCARTLRGKT
                     EGFGLVLLEAAAQACPSIVTRVGGMPEVVRHEETGLVIEEADLDGLTAGLRNLFANRS
                     LRDEMGRRAYEHAKTFTWDACARATYRAILKGY"
     gene            complement(3668..4483)
                     /locus_tag="AC159B_03365"
     CDS             complement(3668..4483)
                     /locus_tag="AC159B_03365"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3376822.1"
                     /translation="MIDFTESLCRREWFATARKTVSVIGERPNLARTIKGTLTGSRRT
                     SRESILRFKHLLPMCPVVLMVGAGTMGAGCEALYENQDVRQIAFDIYPSNLTQFVADA
                     HQIPLADNSVDGVIIQAVLEHVLEPGAVVAEIYRVLKPGGIVYAETPFMQQVHEGAYD
                     FTRFTELGHRWLWRQFEEIDRGVIGGPGLSLYWSARYFCRAVIGRQRLADAISFPLIG
                     LSIFDRFLSLNKKIEGANGVRFLGRKVDRSLPRETLISCYLGSAPTALTDLRR"
     gene            complement(4633..>5155)
                     /locus_tag="AC159B_03370"
     CDS             complement(4633..>5155)
                     /locus_tag="AC159B_03370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3376823.1"
                     /translation="LRDYFVHDGPIVPSTVVLRSSVLEEVGLFDESFRVGEDTEFYLR
                     VAEKWLFCHVPGAFTFKRRHAGQLSTRLDALLPDATRVTQTFVSSHSELGFLAGRRMA
                     RRHAKVSVDCAMRGEWRQALSHDFRAISLAPLYWRAWANLVLLLAPAFVVRPLYKALK
                     RPWHALRQSRHSA"
BASE COUNT         1111 a         1592 c         1573 g          879 t
ORIGIN      
        1 cgaaggtggc gacgagcgcc actgacgttg gaacacccac aaatcccatc ttgaagctca
       61 tgcccgtgcc gacgatgacg gcgctgatcg cagcggggcg cacatgaact tcgacgatgc
      121 tcctgaaaga caagtcgatg gcatggctca cggcgatcaa catggccgcg gacaccgaag
      181 ccatgacagc taagacaacg ccgcagatcg cgttaagacc gtaaggtgct gcataccaga
      241 tgcccagaac gagagttgcc gcgcggagag catgtacaat cgaattcacg tgaggccggc
      301 cgagcgcctg aaacagagcg ccacaagcgc gccacgccag gcggaaatag agcgcacccg
      361 ataggaccgc gaacggtacc actgccccgc tccaatgcgc tccgagtaga aggttcacaa
      421 gttcgttagc gaatgtcatg cagaaggcgc tcgcaggcag agttagcagt gcgacaacgg
      481 cgagtgtttt cagaagtgca ttgcgcagcc gttctgcatc ctcctgtact cgggtcatgg
      541 cagggaaaac tgacaagcga gtgatgttcc caaagagatt tgccgccgtc gtcgtgagga
      601 atgacgcgcg actgtagagg cctaaggccc cggcccccag caaacggcca actaggaatc
      661 tatcggtgtt ttcggcgata tagacgaagg gctgattcaa cgtgaatcca aagctcaacg
      721 gccaaagctc ggcgaatgcc tgacgagaga accgcggtcg gacgaggagc ccccgcgcgg
      781 ctatgcccag gaccagcgcg gtcacgaacg tgtccgcgac ttgcatggcg acgagtgacc
      841 agtagccaaa cccataccag gccataggga tggcaacgag aaacgtcgaa atcgtgaagc
      901 tgaagagggg acgcaacgcc acgctcttga cctgcatgtt tcgggcaagt agcgcctcgc
      961 agagaacgct gaacgctccg aagggtgtga gacaggcgag gaccttcaga atctgcgtga
     1021 gttccggtat ccgcatcaac tccgcaacca atggagcggc gacccacaat ccggccccgg
     1081 caagcagcgc catgaagagg gcggacgaaa gggcagttgc cacgtggtcc gatcgcacgt
     1141 ccttgcgctg gatcagcgtc ggcgccagcc ccatcccgta gatcatcgcc gcgaaatcca
     1201 tcgcgatcag agcacccgca acgaccccgt actcgtgcgg tgtcaagagc cgggccagga
     1261 tggcgatcga gacgacgcgc aaggcgagcc gcgcgataga agcccctccg atccaggccg
     1321 cacccttcag ggtccgcgct gtcaggctct cgcgcatggt cccgcttcga tgaggtcgcg
     1381 gtacagcgtc aagtgccgct cgacgacttt ctcgagcgag aagcgggcgc gtattgtctc
     1441 ccgcgccgcc tcacccgtcg cgcggcgacg ctccgctgtc atcgcaagag acgtctcgag
     1501 ccgggtagcg agcgcggtct catcgccccc cgggaacaag aaccccgaca cgccgtcctc
     1561 gatcagatcg tcggtgccgc tcactcgtga aaccaggggc atcaccccat gactcatcgc
     1621 ttcgaggagt gcgttcgaca ttccctcgga cagcgacgtg gagaggtaga cgtgcgccgc
     1681 tttgagatat ggcgtgacgt cgtcgaccgc gcccgtgaag acaatgcgcc tctcttgtcc
     1741 gaggcgtcgg ctcaacgcct ccaactcagg gcggcagggt ccgtctccga ggatcgtcag
     1801 atgagccggc gtttcggctg gcaagcgcgc gaacgcacga atcgtctggt cgacggcctt
     1861 ctcggcgtcg agccggccca tggataagaa atggacgacg ccgttgcttg atcgccgcct
     1921 cccatgcgcc gggatgtcga tgccgttcgg aatggagtga atgcgctccc gaggcacgcc
     1981 ccagcgtgag agatctgccg cgatctctcg gctgttggct acccaagcgg tggtgttgct
     2041 tgcgatgcgc tgggcgaaga acgagccgac cagccgcttg cgctgaacca tcgagaggtc
     2101 gaaatgcgtt tcaccgcccc gccccggctt cacgacaacg ggcttgccga gccacttccc
     2161 tgccaccacc gccggaaacg cgtgcaggcg gccatggacg acgtaaatca ggtcatagct
     2221 cagtctgttt cgccacaacc aggaagagat gcacaggcac caacacagga acgcatcgag
     2281 gtgccttcca ccgagattcg gggccgcgag cagccggaag cgacgcaccg aaactggccc
     2341 ctcattctcc cggggcggcg ttctgctctc caagcgtggc gcaagtagcg tcaccccggc
     2401 gccgcggcgg gccaacgctt gcgccagtcg gcgcgtctgc tgctcggcac cgccgaaggt
     2461 ctccggcaga tacgcgggca ggatcatgac aattcggggg ccggttagag tcattaataa
     2521 gtcgttacgg cttgtcaata gcccttcaaa atggcgcgat aggtcgctcg tgcacatgca
     2581 tcccatgtga aggttttcgc gtgctcatac gcgcgtcggc ccatctcatc acgcaaggac
     2641 cgattcgcga acaaatttcg cagaccggca gtcaatccat ctaagtccgc ctcttctatg
     2701 accagtccgg tctcttcatg gcgcacgacc tccggcattc cgccaacgcg cgtgacgatc
     2761 gacggacacg cctgtgcagc ggcttcgagg agtaccaggc cgaagccctc tgtcttgcca
     2821 cgcaaggttc tggcgcagag aacatggcat acggaggtgg catagagacg tctcaaatcc
     2881 tcgttgctgg ttgattctgc aaacaaaaca cgcaaccctg tcttcaagac gaagcgttct
     2941 agcgatctac gataatcagc atcaatcacc ggaccggcga tgacatagcc gatttctacg
     3001 cccgttgctt tgacagctct taacgcggcc aggacgtgct ggtgcccctt gcggggctcg
     3061 agccttccca cggtgcagat gcatggaccg gctgatagaa atcgttcgag tcggctgtcg
     3121 gtgcaagcgg caggaggtgc ggagaaccac tctcctcgca ctcccagatg agcgatccgc
     3181 agctctcgat gggaaatctg ggcggaggcg accctcgcgg tagcatgtga gttagcgaaa
     3241 acgaccgcag cgcgacgata gccggccctg gccgacttga cgcgaaggcc cttcccgcca
     3301 agcttcgcag cctcgctccc gtggatcgtc gctccatagg gccgcccggt cagcagatgg
     3361 gcggtatgca atatcagagt cgagcggatg tccacgccgt gccagaatgc atcgttcgga
     3421 gtcgcgagta ggcgtgctag cagttccgca gccgatccag gaccgagcct ttgatgccgc
     3481 aaaatgcgga tgacctcgaa acgagtttcg ttcgtctccc tttcataggc cggcgcgaga
     3541 acggttactt cgatgccagt cgccgagaca gcggccgcga gatcagctgc gtaggtcgcc
     3601 atgccgccag tgaacggggg gaactcatgt gtgatcatta ctagcctcgt gaaatcggtg
     3661 gacacgctca gcgcctaaga tcagtcaagg ccgtaggagc cgatcccaga tagcacgaga
     3721 tcagagtttc acgcggcaac gaccggtcga ctttccggcc caggaagcga accccattcg
     3781 ctccttcgat ctttttgttg agtgacaaaa agcgatcgaa gatcgaaagg ccgatgagag
     3841 gaaaactgat ggcgtcggcc agacgctggc gtccgatgac ggctcggcaa aaataccgcg
     3901 cactccagta gagagagaga ccgggtccgc cgatcacgcc gcggtcaatc tcctcgaact
     3961 gccgccagag ccatcgatgt ccgagctcgg tgaagcgggt gaagtcgtag gcgccctcgt
     4021 gcacctgctg catgaagggc gtttcggcat agacgatgcc accgggtttg aggacgcggt
     4081 agatctccgc aaccacggcc cctggctcca aaacatgctc cagcacggcc tggataatca
     4141 ccccatccac gctgttgtcc gcaagaggaa tctggtgcgc gtccgccacg aactgggtga
     4201 gattggaggg gtagatgtcg aaggcgattt gtcggacgtc ctggttttcg taaagagcct
     4261 cacaacccgc tcccatcgtg ccggcgccca ccatcagcac tacggggcac atcggaagca
     4321 ggtgcttaaa ccgaaggatg ctttccctgc tcgttcgccg agagcctgtt agcgtcccct
     4381 tgatcgtgcg cgccaaattc ggccgctccc cgatgacgga cacggtcttg cgcgctgtag
     4441 cgaaccattc cctgcggcac aagctctccg tgaagtcgat caagacgggt tgccccgcaa
     4501 cgacaggaaa cttctcgccg tcagcgctta cgcaatcaag gttggcgccc tccgacacca
     4561 gcgaaaggcg cttgcgcggt tgaccagggt atcgaagtag ctcgccaatg gatacgagag
     4621 ccacaagcat ggtcaggcgg aatgccgaga ctggcggagg gcgtgccagg gcctcttgag
     4681 cgccttgtat aagggccgca ccacgaacgc tggcgcgagc agaaggacca agttggccca
     4741 ggcacgccag tagagcggcg ccagactgat cgccctgaag tcgtgactga gggcctggcg
     4801 ccattcccct ctcatggcgc agtcgacact gacttttgcg tgccgtcgcg ccattcggcg
     4861 gccggcaagg aacccgagct cggaatggct tgagacgaag gtttgcgtaa ctcgagtagc
     4921 gtcgggtaga agagcgtcga gtctcgtgga gagttgaccg gcatgccgac gcttgaaagt
     4981 gaaggcgccc ggcacgtggc agaacagcca cttctccgcg actcgcagat aaaactcggt
     5041 gtcctcgccg acgcggaagg attcatcgaa gaggccgact tcctcgagaa cggagctgcg
     5101 gagcacgacc gtggacggca cgatcggccc gtcgtgcacg aagtaatcac gaagg
//
LOCUS       DBNGSB010000122         3136 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_854566, whole genome shotgun sequence.
ACCESSION   DBNGSB010000122 DBNGSB010000000
VERSION     DBNGSB010000122.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_854566"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1205
                     /locus_tag="AC159B_03375"
     CDS             <1..1205
                     /locus_tag="AC159B_03375"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val family dehydrogenase"
                     /protein_id="HIR3376824.1"
                     /translation="RAADLLPLKRGIREFLVQPHRELTINFPVKMDDGTVKVFTGYRV
                     HHSTVLGPTKGGIRYYPHVDLDEVRALAMWMTWKCALMHLPYGGAKGGVACDPSSLSK
                     GELERLTRRYATEISLFIGPDRDIPAPDVGTNDQIMAWIMDTFSMHAGHSTPAVVTGK
                     PISIGGSAGRREATGRGVMITAREAAKARGISFSGSHIAVQGFGNVGSVAAYLMHDQG
                     CRVVAVSDVQGGIYNPDGLDPRAALDYLKETGSVAGFPGSEAVSNAELLELPCDFLVP
                     AAIEGQITGENAARIKARIIVEGANGPTTPDADEILQERGVFVVPDILANAGGVIVSY
                     FEWVQDLASFFWSEAEINERLERIMEQSFDGVAGLAKEREIDLRTSALLFAVRRVADA
                     LMTRGIYP"
     gene            complement(1196..2425)
                     /locus_tag="AC159B_03380"
     CDS             complement(1196..2425)
                     /locus_tag="AC159B_03380"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003351687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR3376825.1"
                     /translation="MKALVFDPTIPRFAVTRVLGLLRRNASWGRFSPLKLADIPEPPL
                     PRGDWVRVQVRLGGICGSDLHTIHLETSPALSALTSFPFVLGHENVGTIAEAGRDVHD
                     LRAGQRVTVEPPLPCQARGVSPPCANCAVGDYHLCLRYTDGHLSPGLMIGACRDTGGS
                     WGTSFVAHRSQVFPLNDGIADENALLAEPMACALHPLLRHPIAPGATVLVIGGGAIGQ
                     CAIASLRATGSNARVIALVKHPFQGEMARRLGANEVVLLGRGDAHYDAIARLTSATLR
                     KPMLGKRVMIGGVDTTIECVGAGRSIDDALRLTRPGGRVVLLGLASIPRGIDWTPIWL
                     KELQVTGSYIYGVENWRGRRIRTMDLVLEWMGQGSVDLSPLVTHRFPLDAYPQALETA
                     MGKAESKAFKVVFIPQG"
     gene            complement(2558..>3136)
                     /locus_tag="AC159B_03385"
     CDS             complement(2558..>3136)
                     /locus_tag="AC159B_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011172859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR3376826.1"
                     /translation="AGKRLDALDLLPAPFPIHELLEERDLRHIERLYGIGGLSYGNLS
                     VRKDKKRFWMSASGVNKGNMKIVGRDMLMVKGFDPKRNVMLLSVPPNVEPRRVSVDAI
                     EHWMIYTEHPSVGAIVHVHAWMGDIKSTTINYPCGTIQLAQSVADVVRDAPDPARAVV
                     GLKNHGLTITGPTLDNIFNRLEEGFIRQVPMS"
BASE COUNT          610 a          982 c          971 g          573 t
ORIGIN      
        1 accgcgccgc cgatctcctg ccgctcaagc gcggcatccg cgagtttctc gttcagccgc
       61 accgcgagct caccatcaac tttccggtca aaatggacga tggcacggtg aaggtgttca
      121 ccggctaccg ggtccatcac agcaccgtgc tcggacccac gaagggcggg atccgctact
      181 acccgcatgt cgacttggac gaagtgcgcg cgctagccat gtggatgacc tggaaatgcg
      241 ccctcatgca cctgccctat gggggtgcga agggcggcgt ggcctgcgac ccgtcgtcac
      301 tatccaaagg tgagctcgaa cggctcaccc gacgatacgc caccgagatc agcctcttca
      361 tcggccccga tcgggacatc cccgcgcccg acgtcggcac caatgatcag atcatggcat
      421 ggattatgga cacattcagc atgcacgcgg gacactcgac gccggcggtg gtgacgggca
      481 aaccgatcag catcggcgga tccgcgggcc gtcgggaagc caccggccgc ggggtcatga
      541 tcaccgcgcg cgaggccgcg aaggcccggg ggatctcgtt ttcgggcagt cacatcgcgg
      601 tgcagggctt cggcaacgtg ggctccgtgg cggcctatct catgcacgat cagggatgca
      661 gggtagtcgc cgtgagcgat gtgcagggcg gcatctacaa tcccgatggg ctggatccgc
      721 gcgccgcact ggactatctc aaagaaacgg gcagcgtcgc cggttttcca ggcagcgagg
      781 cggtcagtaa tgcggagctc ttggaattgc cgtgcgactt tcttgtgccc gccgcgatcg
      841 aaggccagat cacgggagag aatgcggcac gcatcaaagc caggattatc gtcgaaggcg
      901 ccaatggacc gacaacgccg gacgccgacg agatcctgca agaacggggt gtgttcgtgg
      961 ttccggacat ccttgccaac gccgggggcg tgatcgtgtc ctacttcgag tgggtgcagg
     1021 acctggcgtc cttcttctgg agtgaagcgg agatcaacga gcggctggaa cggatcatgg
     1081 agcagagctt cgacggcgtt gcggggctcg cgaaagaacg ggagatcgac ctgcggacat
     1141 ccgcgctctt gttcgcagtc aggcgcgtcg ccgacgcgct catgacccgc ggcatttacc
     1201 cctgaggaat aaacaccact ttgaacgctt tggactccgc tttgcccatc gccgtctcca
     1261 gcgcttgcgg gtaagcgtcc aacggaaacc ggtgagtcac aagcggggag agatcgacac
     1321 ttccctgccc catccactca agcactaaat ccatggttct gattctcctc ccccgccaat
     1381 tctcgactcc gtaaatgtag ctgcccgtga cctggagttc cttgagccag atcggcgtcc
     1441 agtcaattcc gcgcggaatt gatgccaggc cgagaaggac aacccggccg ccaggacggg
     1501 ttaggcggag ggcgtcgtcg atgctccggc cggcgccaac gcactcgatc gttgtatcca
     1561 caccgccgat catcacgcgt ttccccagca tcggctttcg cagtgttgcg ctcgtgagcc
     1621 gcgcaatcgc gtcgtagtgt gcgtcaccgc gcccgagcag aacgacttca ttggctccga
     1681 ggcgcctggc catctcaccc tgaaacggat gcttgacgag cgcgatgaca cgcgcgttgc
     1741 ttcctgttgc acggagcgac gcgatggcgc actggccgat ggcgcccccg ccgattacga
     1801 gaacagttgc ccccggagca attggatgac gcagcaaggg gtgaagtgcg cacgccatcg
     1861 gctctgccag cagcgcgttt tcatcggcaa tcccgtcatt gagtggaaac acttgggacc
     1921 ggtgggccac gaaggacgtt ccccagcttc ccccggtgtc gcggcatgcg ccaatcatca
     1981 gcccaggact gagatgccca tcggtgtagc ggagacacag atgatagtcg ccgacagcgc
     2041 agttagcgca gggtggggag accccacgcg cctggcacgg cagaggcggt tccaccgtca
     2101 cccgctggcc ggcgcgcagg tcatggacgt cgcgccccgc ttcggcgatc gtcccgacgt
     2161 tctcgtgccc caatacaaac gggaacgagg tgagcgcgga gagggccgga ctggtctcca
     2221 aatggatcgt gtgcaggtcg ctgccgcaga tcccgccgag cctcacctgc acgcggaccc
     2281 agtccccccg cggcagtggc ggctcaggaa tgtcggccaa ctttaagggc gaaaaccgac
     2341 cccacgacgc gtttcgtcgt aataagccaa gcactctggt aaccgcgaaa cgcggaatcg
     2401 taggatcaaa aaccaaagcc ttcatatgcg tctacccagt cttccccgtc ttccctgtct
     2461 tccccgtctc aatcgacaga tcacgagggt ggggtatggt atttcaatct ttagactggg
     2521 gagactgggc agacggggta gacggggaag acggaggtca agacatcgga acctggcgga
     2581 tgaaaccttc ctccagccgg ttgaagatat tgtccagtgt gggaccggtg atcgtcagac
     2641 catgattctt cagcccgacc acggcgcgtg cgggatcagg cgcgtctcgc acgacgtcgg
     2701 ccacggattg cgccagctga atcgtcccgc atgggtagtt gatggttgtc gatttgatgt
     2761 cgcccatcca tgcatgcacg tgaacgatcg cgccgacgga tggatgctcc gtatagatca
     2821 tccaatgttc gattgcgtcc acggaaacac gccggggctc cacgttaggc gggacgctca
     2881 acagcatcac gttgcgtttg gggtcgaagc ccttgaccat cagcatgtcg cgcccaacga
     2941 tcttcatgtt gcccttgttc acgccactcg cgctcatcca gaaccgcttc ttgtctttgc
     3001 ggacgctcaa gttgccgtag ctgagccccc cgatgccgta cagacgctcg atatggcgca
     3061 ggtctcgttc ttcgagcagc tcgtggatcg ggaacggtgc gggcagaagg tcgagcgcgt
     3121 cgagccgttt gccggc
//
LOCUS       DBNGSB010000123         6944 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3462006, whole genome shotgun sequence.
ACCESSION   DBNGSB010000123 DBNGSB010000000
VERSION     DBNGSB010000123.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 6944)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6944)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6944
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3462006"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1317
                     /locus_tag="AC159B_03390"
     CDS             <1..1317
                     /locus_tag="AC159B_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010887346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HIR3376827.1"
                     /translation="NIHLPDDSVRGGQVIEVSERNAVIQVFEETRGLDLAKTSISLRE
                     DLARIGVSRDMIGRRFSGLGDPIDGLPPIIPEKRLPIVGEPINPVARQRPQEFIQTGI
                     SAIDGLNTLVRGQKLPIFSGAGLPHNEIAAQIARNAKVLGTQEQFSVVFAAMGITQRE
                     ATFFIEQFESTGALARSVVFLNLADDPAIERLITPRMALTAAEYLAFELDMQVLVILT
                     DLTNYCEALREIGAAREEIPGRRGYPGYMYTDLATIYERAGRIHGRTGSITQLPILTM
                     PDDDITHPIADLTGYITEGQIILSRELHRLGIYPPITPLRSLSRLMNDGIGKGRTRED
                     HSGTRDQVYSAYANGVDLRRLVAIIGEEALTERDRLYLKFSEEFEKQFLNQGQADRSI
                     EDTLNLAWKLLSTFPKGELKRVKQDHIDKYYFGELMEEVWRDKTRV"
     gene            1413..2057
                     /locus_tag="AC159B_03395"
     CDS             1413..2057
                     /locus_tag="AC159B_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013614016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HIR3376828.1"
                     /translation="MPEAVSPTRMNLLQRKQQAKIATQGVDLLKRKRDALVADFFNIV
                     RQSLAAREGLTKTSEEAYVMLALAKAWEGREALEAAAMADRREVLVDIQVRNVWGTKI
                     PEVGVKEVRRTLLERGHNPTSTSLRTVESASNFEDVLHSILEVAATEIKLKKIGEEIK
                     KTTRRVNALEQVVIPRLIGEIRFITAVLEQRAREDVFRLKRIKQKLEAREQAAT"
     gene            2172..2924
                     /locus_tag="AC159B_03400"
     CDS             2172..2924
                     /locus_tag="AC159B_03400"
                     /inference="COORDINATES: protein motif:HMM:NF017734.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HIR3376829.1"
                     /translation="MSERTLRTFRVSRWHVAFALLLATAGFLAVTQIRNALLIRQQLN
                     VPPDRLQELAFLLRQQESNRAALEQQIFTLRDQLEDYERGVVQNIEKLEDLRKDLERL
                     RILAGLAAMAGPGLVVELDDSPQPLRPGDDPNTVILHYTDLQVVVNDLWASGAEAVAI
                     NGERIVSTTGINCVGTTILCNTKRMAPPYRITAIGDAESMQTALDRSDGSLATLRGFG
                     FPVKLVRGPRLAVPAYRGSFRFSHATPVDLPK"
     gene            2944..3327
                     /locus_tag="AC159B_03405"
     CDS             2944..3327
                     /locus_tag="AC159B_03405"
                     /inference="COORDINATES: protein motif:HMM:NF018632.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HIR3376830.1"
                     /translation="MWLPIAGLLVGILLGFLFSVEIPLGIVKYTAIAILAAIDTILGG
                     LRARLEDRFDLPIFVSGFFANTFFAALLTVIGDLLGVDIYLGALIAFSIRIFGNVGFI
                     RRDLVEGYWRGLTRQERESVFSGRQ"
     gene            3346..4356
                     /gene="gap"
                     /locus_tag="AC159B_03410"
     CDS             3346..4356
                     /gene="gap"
                     /locus_tag="AC159B_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020879752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIR3376831.1"
                     /translation="MVRVGINGFGRIGRQSLRAIMERYRGTIQVTAINDIADIVTNAH
                     LFKHDSNYGTFPGKVQGRNGELVIGDWTIRAFSERDPAAIKWNEADVDLVVESTGRFT
                     AAEKARGHLESGAKKVVISAPAKGEDITVNMGVNHELYDAKKHTIVSNGSCTTNCLSV
                     TAKVLSREFGIKRGFMTTVHSYTNTQRILDVVGENLREARAAAINIIPAATGAAKAIF
                     LVLPELKGKMHGMALRVPTPTVSIVDLVCELDKPATADQVNAAYRRYSEEEMHGYLGY
                     GEEELVSMDYKGSPYSGIVDAPLTTVITENLVKVLSWYDNEWGYASRVADLVNYMAQK
                     GL"
     gene            4435..5616
                     /locus_tag="AC159B_03415"
     CDS             4435..5616
                     /locus_tag="AC159B_03415"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIR3376832.1"
                     /translation="MHKKTIRDVDVKGKRVFVRVDFNVPLDDGRIADDRRIREALPTI
                     TLLRERGARVILASHLGRPQGKVDDSLRLDPVAEKLGELLGANVRKLQDCVGPEVEAA
                     IAAMKDGGVVLLENLRFHKGEEANDPGFAAALAALADLYVNDAFGTSHRAHASTVGVA
                     KLLPAVAGLLLEKELTYLSRALEDPNRPFVAVLGGKKVSDKIGVIRNLLTKSDALLIG
                     GAMSYTFLKAKGFEVGKSLCEDDKLDLARSLIADAGKRRLAFHLPGDIVVADRFAEEA
                     AHKTVPAEAIPKDWMGMDIGPHTSAAYTQVIRDAQTVMWNGPMGVFEMPAFAAGTRAV
                     ARAMAQSRGVTIVGGGDSAAAVEQEGLADKMTHVSTGGGASLEFLEGKELPGVAVLAD
                     R"
     gene            5613..6383
                     /gene="tpiA"
                     /locus_tag="AC159B_03420"
     CDS             5613..6383
                     /gene="tpiA"
                     /locus_tag="AC159B_03420"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIR3376833.1"
                     /translation="MSRRPLVAGNWKMHKTAADAVRLIEDLRKAALPANVEVAVCPPF
                     TALADSTRALAGSSIALGAQDMFWEAQGAFTGEISAPMLVAAGCRYVILGHSERRQFF
                     AETDASVARKIRTAYAHDLVPILCVGERLKEREAGRTDHVVSEQTEAATAELSGEQVR
                     TLVVAYEPVWAIGTGRSATGEEANRVIGLIRRVLGGRTGDASAQTRILYGGSITPDNA
                     AEFFGQPEIDGGLIGGASLDALKFTAIVRAASLEREHM"
     gene            6484..6702
                     /gene="secG"
                     /locus_tag="AC159B_03425"
     CDS             6484..6702
                     /gene="secG"
                     /locus_tag="AC159B_03425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HIR3376834.1"
                     /translation="MENAVLIVHFILAFLVVGVILLQGPKGEGLGAIGGSARLFHGPR
                     PRETLFTRVTAVTAILFALTSTYLTFLR"
     gene            6710..6793
                     /locus_tag="AC159B_03430"
     tRNA            6710..6793
                     /locus_tag="AC159B_03430"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6744..6746,aa:Leu,seq:gag)
BASE COUNT         1379 a         2072 c         2214 g         1279 t
ORIGIN      
        1 aacattcacc tgccggatga ttcagtccgc ggcggtcagg tgatcgaggt ctcggagcgc
       61 aacgccgtca tccaggtatt cgaagagacg cgggggctgg atctcgccaa gacgtcgatc
      121 agtctgcgtg aggatctcgc gcgaatcggt gtatcgcggg acatgatcgg ccgtaggttc
      181 agcggcctcg gtgatcccat tgatggcctg ccgcccatca tccccgagaa gcgcctcccg
      241 atcgtcgggg agccgatcaa cccggtggca cggcagcggc cgcaggagtt catccagacc
      301 ggcatctccg cgatcgacgg cctcaacacg ctggtccgtg ggcagaagct gccgatcttc
      361 agcggcgcgg gtctcccgca caacgagatc gccgcgcaga tcgcgcgtaa tgcgaaggtg
      421 ctcggcacgc aggagcaatt ctcggtcgtc ttcgccgcca tgggcatcac gcagcgcgag
      481 gccacgttct tcattgaaca gttcgagtcg accggcgcgc tggcccggtc cgtcgtcttc
      541 ctcaatctcg cggacgaccc ggcgatcgaa cgtctcatca ccccgcgcat ggctctcacc
      601 gccgccgagt atctggcgtt tgaactcgat atgcaggtgc tcgtcatcct caccgatctc
      661 accaattact gcgaggccct gcgcgagatc ggcgccgcgc gtgaggagat tcccggccgc
      721 cgtggctatc cagggtacat gtatacagac ctggccacca tttacgagcg cgccggccgg
      781 attcacggcc gcacggggtc gatcacgcaa ttgcccatcc tgaccatgcc ggacgatgac
      841 atcacgcatc ccatcgcaga tctcacgggc tacatcacgg agggtcagat cattctcagc
      901 cgggagctgc accgtctcgg gatctatcca ccgatcacgc cgctccgcag cctctcccgc
      961 ctgatgaatg atggcatcgg caaaggacgc acgcgcgaag atcacagcgg cactcgcgac
     1021 caggtgtact ctgcctatgc gaacggcgtc gacctgcgcc ggctggtcgc gatcatcggg
     1081 gaagaagcgc tgacagagcg cgaccgtctc tacctgaagt tctcagaaga gttcgaaaag
     1141 cagttcctca atcagggcca ggcagaccgg tcgattgaag acacgctgaa cctggcctgg
     1201 aagctgctgt cgacgttccc gaagggcgag ctcaagcgcg tcaagcagga tcacatcgat
     1261 aagtactact tcggcgagtt gatggaggaa gtgtggaggg ataaaacccg agtctaagag
     1321 ccgggaacag ggaacgggga acagggaacg gtaaaaggat gtagtgaccg ttccccgttc
     1381 gccgttcccc gctcccgaag tcgaggttaa cagtgccaga ggctgtaagc ccaacacgca
     1441 tgaacctgct gcagcgcaag cagcaggcca agatcgccac gcagggcgtg gatctgctca
     1501 agcgcaagcg cgacgctttg gtcgccgact ttttcaacat cgtgcgccag tcacttgctg
     1561 cccgagaggg gttaactaag accagtgagg aggcatacgt gatgctcgcc ctggcgaagg
     1621 cctgggaggg acgggaggcg ctggaggcgg cggccatggc cgaccggcga gaggtgctgg
     1681 tggacattca ggtgcgcaat gtctggggta cgaagatccc cgaggttggg gtgaaggaag
     1741 tccggcggac cctgctcgaa cgcgggcaca atcccacgtc aacgagtctc cgcaccgtcg
     1801 agagcgcgtc aaatttcgaa gatgtcctgc actcgatcct ggaggttgcc gcgaccgaaa
     1861 tcaagttgaa gaaaatcggc gaggaaatta agaagacgac ccggcgcgtc aacgcgttgg
     1921 agcaagtcgt gatcccccgc ctgatcggcg agatccgctt tatcaccgca gtcctcgaac
     1981 agcgcgcccg cgaggatgtg tttcgcctta agcgcatcaa acaaaagctc gaggctcgcg
     2041 aacaggccgc cacctgacgt ctctcccatc taccctgtct ccaccgtctc atgaaagttt
     2101 ctcgtggtaa gggtatgatt gagactggga agactgggca gacaggggag acggggtaga
     2161 cgtacttggt cttgagtgag cgtaccctcc ggacgtttcg cgtatctcgc tggcacgttg
     2221 ccttcgcgct gctcctggca acggccggct ttcttgctgt gacccagatt cgcaatgcgc
     2281 tgctcatccg ccagcagctc aacgtcccac cggaccggct ccaggaactg gcgttcctgc
     2341 tccggcagca agagagcaac cgcgctgcac tcgagcagca gattttcacg cttcgcgacc
     2401 agctggaaga ttatgagcga ggcgtggtcc agaacatcga gaaactcgag gatctccgga
     2461 aggatctgga gcgtctgcgg attctcgccg gcctggcggc catggcagga cccgggctcg
     2521 tcgttgagct cgatgacagc ccgcaaccgc tccgccctgg agacgatccc aacaccgtaa
     2581 tcctgcacta cacagatctg caggtggtgg tcaacgacct ctgggccagc ggcgccgagg
     2641 ccgttgcgat caacggcgag cgcatcgtgt ccacgacggg tatcaattgt gtcggcacga
     2701 ccatcctgtg caacacgaag cggatggcgc ccccgtacag aattacggcg atcggcgacg
     2761 cggaatcgat gcagactgcg ctggaccgat ccgacggaag cctcgcgacc ctccggggct
     2821 tcgggtttcc cgtgaagctg gtgcggggac cgcggctcgc tgtgcccgcg tatcggggca
     2881 gttttcgctt ttcacatgca acgccggtgg acctaccaaa gtaagtgaaa aggggataag
     2941 tggatgtggc ttccaatcgc agggctgctc gttgggatac tccttggatt cttattctcc
     3001 gtcgagatac cattagggat cgtcaagtac accgccatcg cgatccttgc cgcgatcgac
     3061 acgattctcg gtgggctgcg ggctcggctg gaagaccgct ttgatcttcc gatcttcgtc
     3121 tcgggcttct tcgcgaatac cttcttcgcc gcgttgttga cggtgatcgg tgacctcctg
     3181 ggcgtggata tctatctcgg cgcgctgatc gcattcagca tcaggatctt cgggaacgtc
     3241 gggttcatcc ggcgcgacct tgttgagggt tactggcggg gcctcacgcg gcaggagcgc
     3301 gagtcggtct tcagcggacg ccaatagtcc agaggagggt gacagatggt tcgtgtcggt
     3361 atcaacgggt tcgggcggat cggccgccag tctctccggg cgattatgga acgatatcgc
     3421 ggcacgatcc aggtcacggc catcaacgac atcgccgaca tcgtgaccaa cgcccacctc
     3481 ttcaagcacg actcgaacta tggaacgttt ccgggaaagg tgcagggccg gaacggtgag
     3541 ctggtgattg gcgactggac gatccgcgca ttctctgagc gcgatccggc cgccatcaag
     3601 tggaacgaag cggatgttga tctggtcgtg gagtcgaccg gccgtttcac ggccgccgag
     3661 aaggcccgcg ggcacctcga gagcggggcg aagaaagtcg tcatcagcgc cccggcgaag
     3721 ggcgaagaca ttacggtcaa catgggagtc aaccacgagc tgtacgatgc aaagaaacac
     3781 acgatcgtca gcaacggatc gtgcacgacg aactgtcttt cggttaccgc gaaggtgctg
     3841 agccgtgagt ttggcatcaa gcggggtttc atgacgaccg tgcactcgta tacgaatacg
     3901 cagcgcatcc tcgatgtcgt cggcgaaaac ctgcgtgaag cgcgcgccgc tgcgatcaac
     3961 atcatcccgg ccgcgaccgg cgccgccaag gccatcttcc tcgtgcttcc ggaactgaag
     4021 ggcaagatgc acggcatggc gctgcgcgtc cccacgccga cggtgtcgat cgtcgatttg
     4081 gtgtgcgaac tggacaagcc agctaccgcg gaccaggtga acgccgcgta tcgccgctat
     4141 agcgaggaag agatgcacgg ctacctgggg tacggcgagg aagagctggt ctccatggac
     4201 tacaaaggca gcccgtattc cggcatcgtc gatgccccgc tgacgaccgt catcaccgag
     4261 aatctcgtca aagtcctctc ctggtatgac aacgagtggg gctatgccag tcgcgtcgcc
     4321 gaccttgtga actacatggc gcaaaaagga ctatagcgga agcgcggaaa cgcggtagcg
     4381 cgggagcgcg accagtgcgc gttcccgcgt ttccctgtcc gcccgcgtgt gaggatgcat
     4441 aagaagacga tccgtgacgt cgacgtgaaa gggaagcgcg tgttcgtccg cgtggatttt
     4501 aacgttcccc tcgacgacgg gcgcatcgcc gacgaccgca ggatccggga agccctccca
     4561 accatcaccc tgctgcggga gcgcggcgcc cgtgtgatcc tggcgtccca tcttgggagg
     4621 cctcaaggaa aggtggatga cagcctgagg ctcgacccgg tcgcggagaa actcggcgag
     4681 ctcctcggcg cgaatgttcg caaactgcaa gactgtgtcg gccccgaagt cgaagctgcc
     4741 attgccgcca tgaaagacgg cggcgtcgtt ctcctggaga acctgcggtt tcacaaaggc
     4801 gaagaggcca acgacccggg gtttgccgca gccctggccg ccctggcaga tctgtacgtc
     4861 aacgacgcct tcggcacgtc gcaccgtgcc cacgcgtcga cggttggcgt tgcaaaactg
     4921 ctcccggcgg tcgccggcct gctgctggaa aaggaactca cctatttgtc gcgcgccctg
     4981 gaagatccga atcgaccgtt cgtggccgtc ctcggcggaa agaaggtctc ggacaagatt
     5041 ggcgtaattc gcaacctcct cacgaaatcg gacgcgctcc tcatcggcgg ggccatgagc
     5101 tatacattct tgaaagcgaa aggcttcgag gtcgggaagt ccttatgcga ggacgacaag
     5161 ctcgatctcg ctcgatcgct gatcgccgat gccggaaagc ggcggctcgc atttcatctc
     5221 ccgggagaca tcgtggtcgc ggaccgtttt gcggaggagg cggcacacaa gaccgtcccc
     5281 gccgaggcca taccgaagga ctggatggga atggatattg gacctcacac ctcggccgca
     5341 tacacacagg tcatccgcga cgcgcagact gtgatgtgga acggtcccat gggcgtcttt
     5401 gagatgccgg cattcgccgc aggcacgcgc gccgtggcgc gggcgatggc ccagagcagg
     5461 ggagtgacca tcgtcggcgg cggagattcc gcggcggccg tcgagcagga aggactggcg
     5521 gacaagatga cccatgtctc gacgggcggc ggagcgtccc tcgagtttct cgaaggcaaa
     5581 gaactcccgg gcgtggcggt gcttgcggat cgatgagccg gcggcctctc gtcgcaggca
     5641 attggaagat gcacaagacc gcggccgacg ccgtgcgctt gatcgaggac ctgcgcaagg
     5701 ctgcgctccc cgcaaacgtc gaggtcgcgg tgtgcccgcc gtttacggcg ctggctgatt
     5761 ccacaagagc gctggctggg tcgagcatcg cgctcggcgc gcaggacatg ttttgggagg
     5821 cgcagggcgc gttcacaggt gaaatctctg cgcccatgct tgtcgccgca gggtgccgct
     5881 acgtgattct tggacattcc gaacggaggc aattcttcgc cgaaaccgat gcttctgtcg
     5941 cgcgaaaaat ccggacggcg tatgcacacg atcttgtgcc aatcctctgt gttggagagc
     6001 gccttaagga gcgcgaagcc ggacggaccg atcatgtggt aagcgagcag acggaagccg
     6061 ccaccgccga gttgtccgga gaacaggtgc gaactcttgt cgtcgcctat gagcctgtct
     6121 gggccatcgg gaccggtcgc tcagcgacgg gggaggaagc gaatcgggta atcgggctga
     6181 tccgtcgagt tcttggaggt cggactggcg acgcttccgc tcagacgagg atcctgtacg
     6241 gtggaagtat tacgccggac aacgctgccg agtttttcgg acagcccgag atcgatggtg
     6301 gtctcattgg cggagcaagc ctggatgcgt taaagttcac ggcgatcgtt cgggcggcat
     6361 cgctggaacg cgagcacatg tgataaaata tcccctggtt gagacacctc ggcgtccgcc
     6421 ggggtgtgtt tgtcataacc ctgaacccga acgacgtgag tggttcaggg ctggaggtat
     6481 caggtggaaa acgcagtctt gatcgtccac ttcatcctgg cttttttggt cgtcggcgtc
     6541 attttgctgc agggaccgaa aggtgagggg ctgggcgcca tcggcggcag cgcccgcctg
     6601 tttcacgggc cgcggccgcg agaaacgctc tttacgaggg tcacggcggt gacggcgatc
     6661 ctgtttgcac tgacctcgac ctacctgacg ttcttgcgat aggaaccgtg ccgggctggc
     6721 ggaattggca gacgcgtacg cttgaggggc gtatgggttt cccgtggggg ttcaaatccc
     6781 tcgcccggca ccatgcacca gtcgctccgc tccggtgcat ggcggctcca tcacatttca
     6841 tctctgacta cgaaactcga ctggtgcatg ccctgtaccc gagcaaagtg agtggtacag
     6901 ggctactttg aacgctacgc tccggtgcat ggcggggcca gcag
//
LOCUS       DBNGSB010000124         3350 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_241775, whole genome shotgun sequence.
ACCESSION   DBNGSB010000124 DBNGSB010000000
VERSION     DBNGSB010000124.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_241775"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..1723)
                     /locus_tag="AC159B_03435"
     CDS             complement(62..1723)
                     /locus_tag="AC159B_03435"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009672788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004467 - long-chain fatty acid-CoA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HIR3376835.1"
                     /translation="MKAVVGHHRPWLRFYEPGVPHTLIYPDSTIPQFLDDTAARHPRG
                     IGTVFFGAQLTFAELHDHARRFAGALRRLGIKPGDRVSLHLPNSPQFVIAYYGALAAG
                     AIVVPFNPLYVEREIEHQLIDSGAEVAVTLDLLYPRLAAVRARTRVREVIVTAINDFF
                     PPLLRLLYPIKARREGHIVAVPAARDIHRFRDLLATGTPENPVQIDPGSTAVLLYTGG
                     TTGVPKGAVLTHRNLVCNVQQARSWFTALRPAKDTLVAVIPFFHSYGMTSAMNFSVAT
                     GTRMVLIPRFQLDMVLKVIDRYRPQIFPGVPTIYSAINAYPDLKGYDLSSITTCISGA
                     AGLPVEVQKRFETTTGGKLVEGYGLTETSPITHVNPINGVRKVGSIGIPVPDTDARIV
                     DANADGVGELAITGPQVMTGYWNQPGETALVLRDGWLLTGDMARMDGDGFFYIVDRKK
                     EMILTGGYNVFPREVEEVLYGHPAVVEAATIGVPDPHRGEVVKAFVVLKEGQRATADD
                     IIEYCRPRLAPYKVPKYVEFRTQLPKSLIGKILRRVLSEEEHTQH"
     gene            complement(1720..>3350)
                     /locus_tag="AC159B_03440"
     CDS             complement(1720..>3350)
                     /locus_tag="AC159B_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014515575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3376836.1"
                     /translation="VAPNLKRLEEKDWKLSRELLRKLGELGFLGVNVPAAYGGVELDQ
                     VTSLVVAEELSLGGGFSVSYGAHVGIGIGPIDYFGNEAQKKRYLPKMVRGELIGAYAL
                     TEPTAGSDAMSIRTRAVLSPDGKHYHLTGAKQFISNATFADVFNVFAKIDGEKHTCFI
                     VERDTPGFSVGEEEHKMGIRSSSTASLYLENARVPVENVLGEIGQGHKIAFNILTVGR
                     FKLAAGCVGAAKYVIKEAVRYAKERHQFGKPIADFGVIKHKLAEMAIRTYVAESMVYR
                     TGGLVEGALQGLHGDPAESMRALEEYAVECAINKVYGSEVLDYAVDENVQIYGGYGFI
                     EEYPAARAFRDARINRLFEGTNEINRLLTTGMLLRRAQRGRLNLIPAAMAVAREITEP
                     GLSEPSDGPLAEEQRLVGMVKKAALFTAGVAVQKYLDQIEHEQEVLSAIADLIIEAFA
                     MESGLLRTQRALERDGAERAGMKVAMIRTYINDAIPRIDATARTVLAAVEEGDTLRTE
                     LAGLRRFLRYTPINTVAQRRRIADAMIAQGRYAT"
BASE COUNT          573 a         1038 c         1056 g          683 t
ORIGIN      
        1 gtgatgggcg ccatcgccat catcgcgggg atcttcgttt tgctcggacg ctgaggaaac
       61 gttagtgctg agtgtgttct tcttccgaca gcacccgccg tagaatcttt ccgataaggg
      121 attttggcag ctgcgttcgg aattcgacgt atttcggcac cttgtagggc gcaagccgcg
      181 ggcggcagta ttcgatgatg tcatccgcgg tcgcacgctg accttccttg agcacgacga
      241 aggctttgac gacctctccc cgatgcggat ccggcacgcc gatcgttgcc gcctcgacga
      301 cggcgggatg gccgtagagc acttcctcga cctcgcgcgg aaagacgttg tacccgccgg
      361 tgaggatcat ctccttcttc ctgtcgacga tgtagaagaa gccgtccccg tccatcctcg
      421 ccatatcgcc ggtcagtagc catccgtcgc ggaggaccag cgccgtctcg ccaggctggt
      481 tccagtaacc ggtcatcacc tgtggacccg tgatggccag ctcgcccacg ccgtcggcgt
      541 tggcgtccac gatgcgcgcg tcggtatccg ggactgggat cccgatactt ccgactttgc
      601 gcaccccatt gatcgggttg acgtgtgtga tgggtgaggt ttcggtgagg ccgtagccct
      661 cgaccagttt tccgcccgtg gtcgtctcaa accgtttctg gacctcgacc ggcaggccgg
      721 cggcgcctga gatgcacgtc gtgatcgagc tgaggtcgta ccccttgagg tcgggatacg
      781 cgttgatcgc cgagtagatc gttggcacgc cggggaagat ctgcggacgg taacggtcta
      841 tgactttgag taccatgtcg agctggaacc gcgggatcaa gaccatgcgc gtgccggtgg
      901 ccaccgaaaa gttcatcgcg gacgtcatcc catatgaatg gaagaatggg atcacggcta
      961 ccagcgtatc cttcgccggt cgcaaggccg tgaaccagga acgggcttgt tgtacgttgc
     1021 agaccagatt gcggtgcgtg agcacggcgc ccttcggcac ccccgtcgtc cctccggtat
     1081 agagcagaac cgctgtggac cccggatcaa tctggaccgg attctccggt gtgccggtcg
     1141 cgaggaggtc tcggaaccgg tgaatgtcgc gtgcggccgg gaccgccacg atgtgtcctt
     1201 cccggcgcgc cttgatcgga tacagcagcc gtagtagcgg aggaaagaag tcgttgatgg
     1261 cggtcacaat gacttcccga acacgagtgc gcgcgcgaac ggcggcgagc cgcgggtaca
     1321 gcaggtcgag cgtcacggcg acctcggcgc cgctgtcaat cagctggtgt tctatttccc
     1381 gctccacgta cagcgggttg aacgggacga cgatcgcgcc ggcggccagc gccccgtaat
     1441 acgcgatgac gaactgcgga ctgttcggca ggtgcagcga gacgcgatca ccgggtttga
     1501 tccccaggcg ccgcagtgcc ccggcgaagc gccgggcgtg atcgtgtaac tccgcaaagg
     1561 tgagctgtgc tccaaaaaac accgtgccga tgccgcgtgg atgtcgcgcc gcggtgtcgt
     1621 cgaggaattg cgggattgtg gagtcaggat agatcagtgt gtgtggaacg ccgggctcgt
     1681 agaaacgcaa ccacggtcga tgatgaccta cgaccgcctt catgttgcgt agcgtccctg
     1741 cgcgatcatc gcgtcggcga tcctgcgcct ctgcgcgacg gtgttgatcg gggtgtagcg
     1801 gaggaaccgc cgcaggcccg ccagctcggt gcgcagggtg tcgccttcct ctaccgcggc
     1861 gagcaccgtt ctggccgttg cgtcgatgcg tgggatcgcg tcgttgatgt atgtccggat
     1921 catcgccact ttcatgcccg cccgttcggc gccgtcccgt tcgagtgcgc gctgtgtgcg
     1981 cagcagaccg ctctccatgg caaacgcctc gatgataagg tcggcaatcg ctgagaggac
     2041 ctcttgctcg tgctcgatct gatcgaggta tttctggact gccacgccgg cggtgaacaa
     2101 cgcggctttc ttgaccatgc cgaccaaccg ctgttcctcg gccaacggac catccgaggg
     2161 ttcggaaagt ccaggttcgg tgatctcgcg ggcgaccgcc atggcggccg ggatcaggtt
     2221 gagccggcca cgctgcgccc gccgcaagag catgccggtc gtgagcagcc ggttgatttc
     2281 attcgtgcct tcgaacagcc ggttgatgcg ggcgtcgcgg aacgcgcgcg cggcaggata
     2341 ctcctcgatg aatccgtagc ccccatagat ctggacgttc tcgtccaccg cgtagtcgag
     2401 cacttctgat ccgtacacct tgttgatcgc gcactccacg gcgtactcct cgagcgcccg
     2461 catcgattcc gccgggtcgc cgtgaagccc ctgaagagcg ccctcgacaa gaccgccggt
     2521 gcgataaacc atgctctccg cgacataggt ccggatcgcc atctcggcaa gcttgtgctt
     2581 gatcaccccg aagtccgcga tcggctttcc gaattggtgg cgctccttgg cgtagcgaac
     2641 ggcttccttg atgacatact ttgccgcgcc gacgcagccg gctgcgagct tgaaccggcc
     2701 gacagtcagg atgttgaaag cgatcttgtg tccctgcccg atctcgccca gcacattctc
     2761 gaccggcacc ctcgcattct cgaggtagag cgaggccgtg gaggagctgc gaatgcccat
     2821 cttgtgctcc tcttccccca ctgagaagcc cggcgtgtcc ctctcaacga tgaaacaggt
     2881 gtgcttctcg ccgtcgatct ttgcaaagac gttgaagacg tcggcgaagg tggcgttgct
     2941 gatgaactgt ttggcgccgg tgaggtggta gtgtttccca tctggggaca acacggcgcg
     3001 tgtgcggatc gacatggcat ctgaacctgc ggtaggctcg gtgagggcgt aggcgccgat
     3061 cagttcgccc cggaccattt tggggaggta gcgtttcttc tgagcctcat tcccgaagta
     3121 gtcgatcggc ccaatgccga tgcctacgtg cgcgccgtaa ctgacgctga agccgccgcc
     3181 gagcgacagt tcctcggcga cgaccaggga cgtcacctgg tcgagctcca ctccaccgta
     3241 ggcggctgga acgttcaccc cgaggaagcc cagctcgccc agctttctca gcagttcgcg
     3301 cgacagtttc cagtcttttt cttccagccg tttcaggttc ggtgcgacct
//
LOCUS       DBNGSB010000125         6106 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_242757, whole genome shotgun sequence.
ACCESSION   DBNGSB010000125 DBNGSB010000000
VERSION     DBNGSB010000125.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 6106)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6106)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6106
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_242757"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..599
                     /locus_tag="AC159B_03445"
     CDS             165..599
                     /locus_tag="AC159B_03445"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3376837.1"
                     /translation="MWGSRSNKVALFKKVPLFDGLTQGQLEQISRLADEIEVREGKRL
                     ATAGETGRELFVIVEGRATVKTPRGRTVRLGRGDFFGEMSLIDGAVRSATVTADGPMK
                     LLVIGQREFWELLNTAPSISKKIMSALAARLREADAAYSKVP"
     gene            681..4064
                     /locus_tag="AC159B_03450"
     CDS             681..4064
                     /locus_tag="AC159B_03450"
                     /inference="COORDINATES: protein motif:HMM:NF037660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3376838.1"
                     /translation="MRCPNCSKENPPDAQFCMRCGTALTRACSVCGTTNSLESQFCLR
                     CGSPLGQATLAERRVLSVLFADLVESTTLSQRFDPERMRVMIGRYFEAMREEIERYGG
                     TVEKFIGDAVMALFGLPVAHEDDPLRAVRAALAMQRRMSELNANQPATFRLRIAVTTG
                     EVVADPLFASSGQFMVTGETVNFAARLQAQAPPDSIVVDERTYDAVEDAVDASALSLV
                     ASGEFSGLPRWQITGLTQRPTVKRLRAKMVGRDEELQFLLALYRRIVDGRRLHVATII
                     GPAGVGKSRLAEEFLHAVTRTPSPAQVLHGRCPAYGEGLTYWPLVEMLKQECDIKDSD
                     AAPVVARKLRERIVAVCAPVLGEEEAEAVAADLATLLGMELTRDYETMWRTRLQTLKY
                     LVENRPAAALREPGVPNEPRHSSETLLQAVRSFLSAKAQHGPLVVLIEDLHWAEESLL
                     ELVERLGARTIEAPILLLCLARPNLQERRTTWGTRLRDYTAISLAPLSAATGERLLDG
                     LVTASAIPEDIRRRVLTHADGNPFFIEEILRMLVDEGNLVQEDSGWRWASDPGEIRIP
                     DTIQGLVLSRLDLLSPLEKRVIQDASVAGRFFWPGAIEAMAGLTSAEATTAIERLQSR
                     ELVEERPVSSLAGEREFEFPHALIREVAYTTLPKLARSDNHRRFAQWVEKNATLSDEF
                     LETLAFHREQAWRYRYETGEPAPQLAREAIDTLREAAKRAVTLGTLPEARRLYERALA
                     VVRNAGLTTDVTLYAELLTDHAEVVKWLRSPAAVFEDTETLFQIAPQIGREDLLARAW
                     LNRAFAEWDKNRLEPAEDAVQRALDLFRAREDRQGEIEALELLGIITHSLRGKLSKAQ
                     AAYRKALELYGEKGDPRGRARVMSQLSRALLDSTGVHEARPLAEEALRLARAHHERIA
                     EATSLYCLGIIEHMAGSSEQAVKHFEEAIAIRRELGDLLGEALWRRLLGMHLLRQGRL
                     DDAERELQTAQALMRERGAPYDNAFLLRGLAELYLARGDLLKAAELGERALAGLSDDD
                     VMAGGTFRATLGKIRAAQGRAADAEQLFRESLEVLKNYEFRFDIALARMKYGEALQML
                     EQQDKASSVLKQARAEFAEIGATNFVREIEQELGSRVAS"
     gene            complement(4061..5647)
                     /locus_tag="AC159B_03455"
     CDS             complement(4061..5647)
                     /locus_tag="AC159B_03455"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR3376839.1"
                     /translation="MTSYDAVIVGAGHNGLVTAAYLARAGLRVVSLERRHVVGGACVT
                     EELWPGYKISTAAYLCGLLQGPIIRDLELERFGYEILPKDPAFFSPFPDGRALFVWQD
                     DARTASEVSRFSPRDARRYPDYEAFLERLAAFVEPWLLRAPPDPIRRRPGDVLGLAQM
                     GWQAMRLPPSDFVRAYRIATQSARDFLDGWFESPELKASLATDGVIGARGGPSTPGTA
                     YVLFHHCMGQAAGKRGLWGFVRGGMGMVTQALAQSAQSHGAQIRTGAEVTQILLRGGA
                     AAGVVLAGGEEITSRVVVSNADPKRTFLNLVPSIDGDFRREIESIRTEGVAMKINLAL
                     DGLPEFRAAPGRGEGPQHRATIHIGPTMEYIDRAWDDARAGRPSADPFLEVTIPSTYD
                     PTLAPPGKHVMNIFAQYAPYRLAGGSWDSLKETFADRVIDTLAEYAPDVQQRILQRHV
                     LSPLDLERDFALTGGDIFHGEMTLDHLLFMRPVPGWADYRTPVRGLYLCGSGAHPGGG
                     VMGAPGHNAAREILKDWKRG"
     gene            complement(5661..>6106)
                     /locus_tag="AC159B_03460"
     CDS             complement(5661..>6106)
                     /locus_tag="AC159B_03460"
                     /inference="COORDINATES: protein motif:HMM:NF015369.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HIR3376840.1"
                     /translation="SSNHFQARAYKIFPPQVRAENADWSRGLMLGELPVNAVVCVPTD
                     GATVPPAVTVRGYALTGGGRRVERVDLSVDRGKSWQTADLGPDEPWAWRLFSHRLTLN
                     PGQHELAVRAWDSAASTQPEDARTLWNFKGYMNNAWHRVRFQVTG"
BASE COUNT         1130 a         1915 c         1993 g         1068 t
ORIGIN      
        1 cgtgatctcg agacagctgc agctcgcgca cgactcaatc tgatacaggt agccgggtcg
       61 ccagaaagaa agagtcgagt tctcctgatg caggagggac aatccctcct gcatcttctt
      121 ttattgagca gggagagtct tctcggtcgt gaaatctgaa gcgcatgtgg ggctcaaggt
      181 caaacaaggt cgcgctcttt aagaaggtcc cattgtttga tggcctcaca cagggccagt
      241 tggaacagat ctcgcggctc gcggatgaga tcgaagttcg cgaaggcaaa cgcctcgcga
      301 cggcgggcga gaccgggcgc gagctgtttg tcatcgtgga aggccgcgcc acggtgaaga
      361 caccacgcgg acgtacggtt cggctcggcc ggggcgactt ttttggggag atgagcctga
      421 tcgacggcgc cgtccgttcg gccacggtca ccgccgacgg gcccatgaag ctcctcgtca
      481 tcggacagcg tgaattctgg gagctcttga atactgcccc atcaatctcc aagaagatca
      541 tgagcgctct cgccgcccgt cttcgcgagg cagacgcagc gtactcgaag gtcccctaac
      601 tacccgcaat aggatcaccg ccattttcgt tgtggctgtt actaccctgt gaccgtgctg
      661 tcgtgctacc gttaatcaac atgcgctgcc ctaactgctc taaagagaat ccgccggacg
      721 cacaattctg catgcggtgc ggcacggcgc tgacacgcgc ctgctcggtg tgcgggacca
      781 cgaacagcct ggagtcgcag ttctgtctcc gctgcggatc gccgttgggg caggcaaccc
      841 tcgcggagcg gcgcgtctta agcgtccttt tcgccgacct tgtcgagtcc accactctca
      901 gtcagcggtt cgatcctgaa cggatgcggg tgatgatcgg acgctacttc gaggccatgc
      961 gcgaggagat cgagcggtac ggcgggaccg tggaaaagtt catcggcgat gccgtgatgg
     1021 ccctcttcgg cctccccgtg gcccatgagg atgatccctt gcgcgcggtg cgcgcggcgc
     1081 tggcgatgca acgtcgcatg agtgagctca acgccaacca gccggctaca ttccgcttgc
     1141 ggatcgccgt caccactgga gaggtcgttg ccgatcccct tttcgcttcg tcaggacagt
     1201 tcatggtgac gggagaaacg gtgaattttg cggcccgcct tcaggcgcag gccccgcctg
     1261 acagcatcgt ggtcgatgag cgcacctacg atgcggtcga ggacgccgtc gacgccagcg
     1321 cgctgtcgct ggtcgcatcg ggagagtttt caggactccc gcgctggcag atcacgggcc
     1381 tgacgcaacg gccgacggtg aagcgattgc gtgcgaagat ggtcggtcgt gatgaagaac
     1441 tccagttcct cctggcgtta taccggcgca tcgtcgatgg ccgcaggctc cacgtggcca
     1501 cgatcattgg tccggccggc gtggggaagt cgcggctggc tgaggagttc ctgcacgcag
     1561 tgacgagaac gccttccccg gcgcaagtgc tgcacggccg ctgtcctgcc tatggtgagg
     1621 gcctgaccta ctggccgctg gtggagatgc tcaagcagga gtgtgacatc aaggacagcg
     1681 acgccgcgcc cgtcgtcgcc cgcaagctgc gggaacgcat cgtcgcggtc tgcgcgccgg
     1741 tgctcggcga ggaggaagcc gaagccgtcg ccgcggacct agccactctg ctgggcatgg
     1801 agctcacacg cgactacgag acgatgtggc gcacgcggtt gcagacactg aagtatttgg
     1861 tggaaaaccg gcccgctgcc gcgcttcgcg aaccgggtgt acccaacgag ccgcgccact
     1921 ccagcgagac actccttcag gccgtccggt catttctctc cgcgaaggcg cagcacgggc
     1981 ctctggtggt gttgattgaa gatttgcact gggcggaaga gagtctgctc gaactcgtcg
     2041 agcggctcgg cgcgcggacg atcgaagcgc cgatcctgtt gttgtgcctc gccaggccga
     2101 atctccagga gcgacggacc acgtggggaa cgcggctgcg tgattacacg gccatctcgc
     2161 tcgcgcctct ctcagcagca acaggagaga ggctccttga tgggctggtg accgcatcag
     2221 cgataccgga agacatacgc cggcgggtcc tcactcatgc cgatggcaat cccttcttca
     2281 ttgaagagat cctgaggatg ctggtggatg agggcaacct ggtccaggag gacagcgggt
     2341 ggcgttgggc ctcagatccg ggggagatcc gcatccctga caccattcag ggactggtcc
     2401 tgtcccgtct cgatctattg tcgccgttgg aaaagcgcgt catacaggac gcgtcggtcg
     2461 ctggacggtt cttctggccg ggcgcgatcg aggcgatggc tggccttacc tcagccgagg
     2521 ccacgaccgc catcgaacga ctgcagtccc gcgagctcgt ggaggaacgt cccgtctcgt
     2581 cactggccgg ggagcgggag ttcgagttcc cccacgcgtt gatccgagaa gtcgcctaca
     2641 ccacgctgcc gaaactggcc cgcagcgaca accatcgccg ttttgctcaa tgggtggaga
     2701 aaaacgcaac cctcagcgac gagttcctcg agacactcgc gttccatcgg gaacaggcct
     2761 ggcggtatcg ttacgaaacg ggagaaccgg cgccgcaact cgcccgtgag gctatcgaca
     2821 cgctgcggga ggcggcgaag cgtgcggtca ctctaggcac gctgcccgag gcgcgcaggc
     2881 tgtacgagcg tgctctggcc gtggtccgca atgccggcct gaccacggat gtcacgttgt
     2941 acgcagaact gctgaccgac cacgccgagg ttgtcaaatg gctgcgctct ccggctgccg
     3001 tcttcgagga cacggaaacc ctcttccaga tcgcgccgca gatcggccgc gaagacctgc
     3061 tggcacgcgc gtggctgaat cgggcctttg ctgagtggga caagaatcgg cttgagcccg
     3121 cggaagatgc cgtgcagcgc gcgctcgact tattccgagc gcgtgaagac cggcaggggg
     3181 agatcgaggc actcgaactc ctgggcatca ttacgcattc gctgcgcggc aagctgagca
     3241 aggctcaggc cgcctaccgg aaagccctcg agctctacgg cgagaaggga gacccgcgcg
     3301 ggcgagcgcg tgtgatgtcg caactcagcc gggccctgct cgatagcacg ggagtccatg
     3361 aggccaggcc cctggccgag gaggcgctgc gcctggcccg agcccatcat gaacggatcg
     3421 cggaagccac gagcctgtat tgcctgggga tcatcgagca tatggccggc tcatccgagc
     3481 aggcggtcaa gcatttcgag gaggcgattg cgattcggcg cgagctcggc gacctgttgg
     3541 gtgaagccct ctggcgccgg ctcctcggca tgcacctgct gcgtcagggg cgtctcgatg
     3601 atgccgagcg cgagcttcag accgcacagg ccctgatgcg cgagcgcggc gcgccgtacg
     3661 acaatgcctt cctgctgcgc ggcctggctg aactgtatct ggcgcgcggc gatctattga
     3721 aggccgcgga actcggggaa cgggccctcg caggactcag tgatgacgat gtcatggcgg
     3781 ggggaacctt ccgagcgacg ctggggaaga tccgcgccgc tcaggggcgc gcggccgacg
     3841 ccgagcagtt gttccgagag agcctggaag ttttgaaaaa ctacgagttc cggttcgata
     3901 tcgcgctggc gaggatgaag tacggcgagg cgctgcaaat gctcgagcag caagacaaag
     3961 cgtcgagcgt cctcaagcag gcacgcgcgg agtttgccga gattggcgcc acgaacttcg
     4021 tacgcgagat cgagcaagaa ctcggcagcc gggtagccag ctagccgcgc ttccagtcct
     4081 tcagaatctc gcgtgcggca ttgtggccgg gcgcccccat tacgccgccg ccgggatgcg
     4141 cacccgagcc acacagatag agccctcgga ccggtgtgcg gtaatcggcc caccctggga
     4201 ccggccgcat gaacagcaaa tggtccagcg tcatctcgcc gtggaagatg tccccgccgg
     4261 tcaacgcaaa atcccgttcc agatctagcg ggctcaacac gtgccgttga agaatccgct
     4321 gctgaacgtc gggcgcgtac tcggccagcg tgtcgatgac gcgatccgca aatgtttcct
     4381 tgagcgagtc ccacgatccg ccggccagcc ggtagggtgc atactgcgca aagatgttca
     4441 tgacgtgctt gcccggcgga gcaagcgtgg gatcataggt ggacgggatc gtcacttcaa
     4501 gaaacggatc tgccgagggg cgtccggccc tcgcgtcatc ccaggcgcgg tcgatgtatt
     4561 ccatcgtcgg tccaatgtga atcgtggcac ggtgctgggg tccttcgccc cgcccgggcg
     4621 ccgcgcgaaa ctccggcagg ccgtcgaggg ccaaattaat cttcatggcg acgccttcag
     4681 tgcggatgct ctcaatctct cgccggaagt cgccatcaat cgacggcaca aggttgagaa
     4741 acgtacgctt cgggtcggcg ttgctgacga cgacgcgcga ggtgatctcc tcgcctcctg
     4801 cgaggacaac accagcggcc gcgccgcccc gaagcagaat ctgtgtgacc tcggctcccg
     4861 tacggatctg cgcaccgtga gattgcgcgg attgcgcgag cgcctgagtg accataccca
     4921 tccctccgcg cacaaagccc cacaggcctc gctttccggc cgcctgtccc atgcagtgat
     4981 gaaagagcac atacgcagtg ccgggagtac ttggtccgcc gcgcgcaccg atgaccccgt
     5041 cggtggcgag cgaggccttg agctcaggcg actcgaacca gccgtcgagg aaatctcgcg
     5101 cactctgcgt ggcgatacgg tacgcccgta caaaatcgga tggcggcaga cgcatcgcct
     5161 gccaacccat ctgtgccaga ccgagcacgt cccctgggcg tcgacggatc ggatcgggcg
     5221 gcgcgcggag gagccacggc tcgacgaatg ccgccaggcg ttccaggaac gcttcgtagt
     5281 ctggatagcg ccgtgcgtct ctcggagaga acctgctgac ctcggacgcc gtccgggcat
     5341 cgtcctgcca gacgaacaag gcgcgcccat cggggaacgg gctgaagaac gcggggtctt
     5401 tcggcaggat ctcgtacccg aagcgctcga gttcgagatc gcgaatgatt gggccttgca
     5461 gaagtccaca gaggtaagcg gcggtactga tcttgtagcc cggccatagc tcctcggtga
     5521 cacaggcgcc gccgacgacg tggcggcgtt ccagcgacac gacgcgtaat cccgcacggg
     5581 ccagatatgc ggcggtgacg agaccgttgt gtccggcccc gacgatcacc gcgtcgtacg
     5641 atgtcatcag gacgcgggcc ctaacccgtc acctggaaac ggacgcggtg ccaggcgttg
     5701 ttcatgtagc ctttgaagtt ccagagcgtg cgcgcgtcct cgggctgcgt gctcgccgcc
     5761 gagtcccagg cccggacggc aagctcatgc tgtccaggat tcagcgtgag gcgatgactg
     5821 aacagacgcc atgcccacgg ctcgtctggg ccgagatctg ccgtctgcca cgattttcca
     5881 cggtccacgg agagatccac gcgctcgacc cgccgtcccc ctcccgtaag cgcatagcct
     5941 cgcaccgtga cggctggcgg cacggtggcg ccgtcggtcg gaacacagac gacggcattg
     6001 acggggagtt cgcccagcat cagtccccgc gaccaatcgg cgttctccgc gcgtacctgc
     6061 ggggggaaga tcttgtatgc gcgcgcttga aagtggttcg aactgg
//
LOCUS       DBNGSB010000126         3510 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_1929888, whole genome shotgun sequence.
ACCESSION   DBNGSB010000126 DBNGSB010000000
VERSION     DBNGSB010000126.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_1929888"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..620)
                     /locus_tag="AC159B_03465"
     CDS             complement(141..620)
                     /locus_tag="AC159B_03465"
                     /inference="COORDINATES: protein motif:HMM:NF019928.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HIR3376841.1"
                     /translation="MPEYGQGPLNAVSIETEVKIEAAPERVFDALVTDISSWWGPAHT
                     FSQEARDVVLEPRLGGRFYEDWGEGEGVLYATVTRLKRPVELGFTGPLGMRGAVVGRV
                     MITLERQGTTTVVRLSHRVVGDVTEETRQSYSGGWQKLLGQGLKPYVERGERTGLKR"
     gene            complement(626..979)
                     /locus_tag="AC159B_03470"
     CDS             complement(626..979)
                     /locus_tag="AC159B_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR3376842.1"
                     /translation="MTIWSHMEHHDLAPAWRALSDPTRRRILDLLKDGPRTTGDLCDE
                     FDVSRFAVMKHLNILERARLVLVERRGRERWNHLNALPIRQIYERWISGYQEHWAASL
                     LRLKRRVESKTGKEQ"
     gene            complement(1025..1381)
                     /locus_tag="AC159B_03475"
     CDS             complement(1025..1381)
                     /locus_tag="AC159B_03475"
                     /inference="COORDINATES: protein motif:HMM:NF013868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR3376843.1"
                     /translation="MTTATLRIERDREIPVVYVQGEIDIVNASHLGAQLFDAAPNDAP
                     GMVVDLSGVTYLDSRGLHLLFELSERLRVRDQRLHLVIPTQGLIRRVLTLTRMDAVVP
                     LYASVPDAVRQMSQTP"
     gene            complement(1378..>3510)
                     /locus_tag="AC159B_03480"
     CDS             complement(1378..>3510)
                     /locus_tag="AC159B_03480"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIR3376844.1"
                     /translation="AALPPNIPADARVTLIDRRGVVLHTTHVGITREQRDVGGRQFVR
                     RALAGQITPVTGPVSLFGQEGRYGAFVPIPSTGWVVGFTRPLAPLEDRLRLLALRQVA
                     VITVAVLAAILIFVMIARRLIGPLRHLAENAIAIGRGERPALGPVEGPAEVSDLSTGM
                     AAMSRAVAHREDTLHFLAEASKEFSRSLDYEATLSNVARLAVPRIADWCVVDIAAEGG
                     EVRRLAVEHVDPAKVELARDVQRRYPTDPESPTGVPNVLRTGRSELYPEITEAMLRQA
                     AKDEEHLRILQKVGLTSVIIVPLPGRDRTLGAITFVNSESGRRFSHADLELAEDLARR
                     AGMAIENARLFARQRGIAETLQRSLLRKDLPEMPGVTAAARYLPAKTEVEVGGDWYDL
                     FALPDSRIAMVMGDVAGSGLEAASVMGQLQHALRAYAQEGHAPASVMERLNMLLELKD
                     MATLVFLIFDPATWTVTYANAGHLPPMTIAPDGRVSMLEGGSPPLGGSPLTMYRESST
                     TLTPGSTILLYTDGLIEVRGESIDVGLDRMSNLLAESGTGDLEALLERILAVMVGGEG
                     AADDVALLALRAEALDPARLRLRIAATPTSLPLVRHTLRRWLGQAGATSEDIYDISVA
                     TAEACANSIEHAYQAGDARLEIDATHRDSQIFVTVRDWGQWREPRGRHRGRGLILMRG
                     LMDTVQVAHSDDGTTVQMRRRLKREARA"
BASE COUNT          629 a         1188 c         1061 g          632 t
ORIGIN      
        1 ccagacgtcg agtgattcga aaggcgtccg caacgcagct cctctcctag gaagctgcga
       61 tcaaattccc gtatgccttt gggtaaatcc ctgacccaac tgggttgttt attggcgcgc
      121 cgcgcgatgc gcgatacgcg ctagcgcttt aagccggtcc gttcgccgcg ttcgacgtat
      181 ggcttgagac cctggccaag gagtttctgc catccaccgc tataggactg ccgggtctcc
      241 tcggtgacgt cccccaccac gcggtgactc aaccgtacga cggtggtagt tccctgccgt
      301 tctaaggtga tcatgacccg gcccacgacg gctccgcgca tccccaaggg tccggtgaag
      361 ccgagctcga ccggccgctt gaggcgggtc acggttgcgt agagcacgcc ctcgccttct
      421 ccccagtcct catagaaccg gccgccgagc ctcggctcta acacgacgtc gcgcgcttcc
      481 tgggaaaagg tatgcgcggg cccccaccag ctcgagatgt ccgtgaccaa agcatcgaag
      541 actcgctcgg gagcagcctc gatcttgacc tcggtttcga tgctgacggc attcaatggt
      601 ccctgcccgt actctggcat gtgcttcact gctcctttcc cgttttgctt tccacccgcc
      661 tcttcagccg caataaggac gcggcccaat gctcctgata gccgctgatc caccgttcat
      721 agatctggcg aatcggcaat gcgttgaggt ggttccatcg ctcgcgtccg cgccgctcga
      781 cgaggacgag ccgtgcgcgc tccaagatat tgagatgctt catgaccgca aaccgcgaga
      841 catcaaactc atcgcagagg tcccccgtgg ttcgcggacc gtctttcagc aggtcgagaa
      901 tccgccggcg ggtgggatca gacaacgccc tccaggccgg cgcgagatcg tggtgctcca
      961 tatgtgacca aatggtcaca tatacttccc gccctgtcaa ggggggcgga tccgtgcatg
     1021 gagtctaggg cgtctgactc atctgccgga cggcatccgg cacggaggcg tacaggggaa
     1081 cgaccgcatc cattcgggtc agcgtcagca cgcgccggat aagcccctga gtggggatga
     1141 ccaagtgcag gcgctgatcg cgcacccgca accgttcgga cagttcgaac aggagatgga
     1201 ggccccgact atcgagatag gtcacaccgg aaaggtcgac gaccatgccg ggcgcgtcgt
     1261 tcggagcggc gtcgaacagc tgcgcgccca ggtgcgaggc attgacgatg tcgatctcgc
     1321 cctgcacgta cacgacaggg atctccctgt cgcgttcgat gcgaagtgtc gccgtcgtca
     1381 tgcgcgggct tcccgtttca gtcgccgtcg catctgcacc gtcgtaccgt catcactgtg
     1441 ggccacctgg acagtatcca tgaggccccg catcagaatc aaccctcgtc cccggtgtct
     1501 gcctcgaggt tctcgccact gcccccagtc ccgcacggtt acgaatatct gactgtctcg
     1561 atgcgtcgcg tcgatctcca gccgggcatc gccggcctga taggcatgct cgatcgagtt
     1621 cgcacaggcc tcggccgtcg caacagagat gtcatagata tcttccgagg tcgcgcccgc
     1681 ctgcccaagc caccggcgca gcgtatgccg caccaggggc agcgaggtcg gcgttgccgc
     1741 aatgcgcagc cggagtcgcg cgggatcgag cgcctccgct cggagggcga gcagcgcgac
     1801 gtcgtctgcc gcgccttctc cacccaccat gacagcaagg atcctctcca gcagcgcctc
     1861 gagatcccca gtgccactct ccgctaacag gttgctcatc cggtctagtc ctacgtcgat
     1921 ggactctccg cgcacttcga tcaggccgtc ggtatacagc aagatcgttg agccgggtgt
     1981 cagtgtggtg ctcgattccc ggtacatcgt gaggggcgag cccccgagcg gcggcgagcc
     2041 gccctccagc atggacacgc ggccatccgg cgcgatggtc atcggtggaa ggtgcccggc
     2101 gttggcatag gtcacggtcc acgtcgccgg atcgaagatg agaaacacca gcgtggccat
     2161 gtccttcagc tcgagcagca tgttgagccg ctccatcacg ctcgcggggg catgtccttc
     2221 ctgtgcgtac gcgcggaggg cgtgttgcag ttgtcccatc accgatgcgg cctcgagacc
     2281 acttccggcg acgtctccca tcaccatcgc aatgcggcta tcggggagcg cgaaaagatc
     2341 gtaccagtct ccaccgacct cgacctcggt tttggccgga agataccgcg ccgccgccgt
     2401 tacacccggc atttccggta gatccttgcg cagcaaactg cgctgcagcg tctccgcgat
     2461 accacgctga cgcgcgaaga gcctggcgtt ctcgatcgcc attcctgccc ggcgcgcgag
     2521 gtcttccgcc agctcgaggt cggcgtggct gaaacgacgg ccggactccg agttgacgaa
     2581 tgtgatcgca cccagcgtcc ggtctcgacc cggcagcggc acgatgatga cagatgtcag
     2641 cccgaccttt tggagaatgc gcaagtgctc ctcatccttt gccgcctgcc tgagcatcgc
     2701 ctcggtaatc tccgggtaca actcggaacg ccccgtgcgc agcacattgg gcacgccggt
     2761 cggtgactcg ggatccgtcg gataacggcg ctgcacatcc cgcgccagct cgactttcgc
     2821 cggatcgacg tgctcgacag ccagccggcg cacctcgcct ccctccgcgg caatatccac
     2881 gacgcaccaa tccgcaattc gcggaacggc gagacgcgca acgttgctga gcgtcgcctc
     2941 gtaatcgagc gaccggctga attctttgct ggcttccgcc aggaaatgca acgtatcctc
     3001 gcggtgcgcc accgcccggc tcatggccgc catccccgtc gacaaatcgg agacctcggc
     3061 cggcccttcg acgggtccca gggccggtct ctccccacgc ccgatcgcaa tcgcattttc
     3121 tgcgaggtgc ctgagcggcc cgatcagccg gcgggcaatc atcacgaaga tgagaatggc
     3181 tgccaggacg gcgacagtga tgaccgcgac ctggcgcaag gccaggaggc gcagccgatc
     3241 ctcaagcggc gcgagcggcc gggtgaaacc gacgacccag cccgttgaag ggatcggcac
     3301 aaacgctcca tagcgtccct cctgaccgaa cagcgatacc ggaccggtca cgggagtaat
     3361 ttgaccggcg agcgcgcggc ggacaaactg gcggccgccg acgtcgcgtt gttcgcgcgt
     3421 gatgccaaca tgtgtcgtat ggagcaccac tccgcggcga tcaatcagcg tgacgcgggc
     3481 gtcggcggga atgttcgggg gcagcgcggc
//
LOCUS       DBNGSB010000127         6977 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3923760, whole genome shotgun sequence.
ACCESSION   DBNGSB010000127 DBNGSB010000000
VERSION     DBNGSB010000127.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 6977)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6977)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6977
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3923760"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="AC159B_03485"
     CDS             complement(<1..578)
                     /locus_tag="AC159B_03485"
                     /inference="COORDINATES: protein motif:HMM:NF015971.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HIR3376845.1"
                     /translation="MFDAPPLNRHSANPILTPTSHWWEDRWVFNCGATIYRGEVHLLY
                     RAQGADWISRLGLARLRNGVEVIERSPRPVFEPEVGNEWERLGTEDPRVTAFNGRYYI
                     CYTAASLYPATEPVRRTRPSPFTDVGVPWRVRVAIATTKDFKHYRRMGLAFKQWDNKN
                     GALFPAKIRGKYYILHRIFPNVHLGVSRDLRR"
     gene            complement(766..1050)
                     /locus_tag="AC159B_03490"
     CDS             complement(766..1050)
                     /locus_tag="AC159B_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012966031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HIR3376846.1"
                     /translation="MATYIMLSRLTEEGAETIRKNPGRVQEVNKELEGMRVRVLQQYA
                     VLGAYDFVNIVDAPDLATVMKASIQLGARGSVRIETLAAVPIDEFVRVVR"
     gene            1169..2137
                     /locus_tag="AC159B_03495"
     CDS             1169..2137
                     /locus_tag="AC159B_03495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019551402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - quinone reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase family protein"
                     /protein_id="HIR3376847.1"
                     /translation="MNAVRIHQPGGPDVLRYEEVPTPLPASGEALVHLRAIGVNFIDI
                     YHRSGQYPVSFPAIIGQEGAGVVDSVGSGVTDVSPGDRVAYSNVFASYAEFVTVPAWR
                     CVKIPASLTFEQAAAAMLQGMTAHYLSHTTYPIKRGDMVLVHAAAGGVGALLVQMAKR
                     RGAKVLATVSSDEKAKVAREAGADVVINYSTVDFVPEVKRLTDAQGVPAVYDSVGRDT
                     FDRSLDCLALRGMLVLFGQASGRVPALDPQVLNAKGSVFLTRPSLHHYTHTREELLSH
                     STEVLQWVGDGTIRLRIDQSFPLSRAANAHEYLQSRRAIGKILLIP"
     gene            2149..3279
                     /locus_tag="AC159B_03500"
     CDS             2149..3279
                     /locus_tag="AC159B_03500"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR3376848.1"
                     /translation="MRPPERLPPPDAPQDVLTTSRVRALLRDWMTASEDPVVAHDAVL
                     RLTYGVTLAVAALALLARLAGMAIPAWLLVALAGTLLLATRVQQLLLRRGRYVRAMPL
                     LHMNLSLLLLSVGIAYSGGLRSPFVWTYAIPVAIEGVLRGGPWGIIGATASTIHLVGA
                     TFAATSGIAGRPFPPEPGLSAFLISYVGMFYVVAFIASSLREQVRDIFSLARTDALTG
                     LANRYALRLSLEREVARARRYGLECGLVVVEIDQFKRFNDRHGHLHGDEVLRRVAATL
                     RGSCRAADLPARFGGDEFVVVLPQTGKGEATRLAERIRGDMEEFTLLRGFGLTVSVGV
                     SAFPDDGLTGRDLLEAADTAMYRAKSGGGNAVAVAAGTHSGS"
     gene            3338..5428
                     /locus_tag="AC159B_03505"
     CDS             3338..5428
                     /locus_tag="AC159B_03505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR3376849.1"
                     /translation="MHSGRPESGHSHQESHTRATQKGDSRAPRWRRAAPKAGVVLLVA
                     VLAVGALAGVAASFSLVPDISKVYDPPSEATRIYAATGELIASVYRENRDYVTLEQIP
                     LVMQQAVIAIEDERFYQHSGIDLQAIARAAWRNLQARELREGGSTITQQLARSVFLTS
                     ERTLKRKVAEALLAIEIERRLSKREILERYLNQVYFGQGAYGIEMAAQVYFSKPASEL
                     GLGESAMLAGLVRAPSRYSPYRNLELARYHQRLVLRRMVQLGYITRERAYEAGARQPI
                     TLARQRNVGLTSIRAPYFVSYILPHLLERYGEEQVYRGGLQVYTTLDPSMQSAADRAV
                     RDGLEEAHNARLNVSQAAVVAIDARSGYIRAMIGGSDWTQTQFNRAYQARRQPGSAFK
                     PFIYTTAIEAGWRPWKMLLDAPVSYWSGAGYWRPKNYDKKFRGPVTLRRALEQSINVP
                     AVRTMRKLGPERVITVARRMGIQSPLEPNLSLALGSSDVTVLEMASAYATLANMGIYS
                     EPIAIRQVVDQQGKVLEQNGPRRQPALEVEVAYRMIDIMKGVITHGTGRAARIGRPAA
                     GKTGTSDDHRNAWFIGFTPHISTAVWVGNDDNSPMRKVVGGTVPARIWAAFMRTAVAA
                     MPADDWDRPEGVEVEVRQAPRAVEVPPPSIEELMRQAIMQQLKQHLERATPVRSNDPA
                     EDGTGRLKPPKDDG"
     gene            5562..6338
                     /locus_tag="AC159B_03510"
     CDS             5562..6338
                     /locus_tag="AC159B_03510"
                     /inference="COORDINATES: protein motif:HMM:NF014582.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIR3376850.1"
                     /translation="MTHFFQIFFLVTFGSVVSLALGLVLVWQQRLFQRLHLYLVAFAA
                     GALIATALFDLLPEAMEGVEASGTSPETPFMWTAAAIAIFFVFEKSLHWYHDHTGHGP
                     EGKVTKAVVPLITLGDGLHNFIDGALIAAATLASPQLGLLTAVAVFLHEIPQEVGDFS
                     VLIFGGLSRGTAILYNLLSAAAAYLGAFATLILSSGATSSLTMPLVALTAGAFLFIAL
                     GELLPELIHKERRGARTVLLTATFIFAMVFMRYLGERLGV"
     gene            6351..>6977
                     /locus_tag="AC159B_03515"
     CDS             6351..>6977
                     /locus_tag="AC159B_03515"
                     /inference="COORDINATES: protein motif:HMM:NF019522.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HIR3376851.1"
                     /translation="MKFAALLVWLLFSSAAAASAQDVEPEPADEYRRGRVVEIVFEEA
                     RELAGYPQFFQRVVVDLGGRRVTAEHSELATDPARRLGVGERVVVASTESGYFVVDRD
                     RLPALAIIFGLFFAIVVAFTRWRGVASVFGMIVSAVVLTTIVVPQILAGRSPLATSIV
                     GALIIAITSIYLAHGVNRRTSVAVVGTLITLGLAGALSVAFVSVARLFG"
BASE COUNT         1199 a         2126 c         2254 g         1398 t
ORIGIN      
        1 caccgcctga gatctcgcga aaccccgaga tgtacgttgg ggaagatgcg gtgcaggatg
       61 tagtacttac cccgaatctt ggccggaaat aacgcgccgt tcttattgtc ccactgtttg
      121 aacgccagcc ccatccggcg gtagtgcttg aaatctttcg ttgtggcgat cgcgacacgc
      181 acgcgccagg ggacgcccac atctgtgaat ggggaagggc gggtgcgccg gacgggctcg
      241 gtggctggat acagtgacgc ggcagtgtag caaatgtagt agcgcccatt gaaagcggtg
      301 acgcgaggat cctcggtgcc gaggcgctcc cattcgttcc ctacttcagg ctcgaagacg
      361 gggcgagggc tgcgctcgat cacctccacg ccgtttcgca ggcgggcaag acccaggcgc
      421 gagatccagt ccgcgccctg cgcacggtag agcaggtgga cttcgccccg gtagatcgtt
      481 gcgccgcagt tgaataccca gcggtcttcc caccagtgag acgttggggt cagtatcgga
      541 ttggcggagt gtcggttgag cggtggcgcg tcgaacatcc catcctccat ttattctcta
      601 cgtccaggaa gattgatttg tgccaagtgg ggcaaaccct tctgtcttgt gcccatttga
      661 ccagaatttt cgtccagggg agtaggcgat tctgtaacaa ttcagccgga cggcagatct
      721 gcgggaggcg ggctgcagcg gtccccgccc cccgcgatga cggggctatc ggacgacgcg
      781 gacaaattca tcgataggta cagcggccag cgtttcaatc ctcacacttc ccctggcgcc
      841 gagttggatg gacgccttca tgaccgtagc caggtcgggg gcatcgacga tattcacgaa
      901 gtcgtatgcg cccaggacag cgtactgctg caagacgcgg acgcgcatcc cctcgagttc
      961 tttgttcact tcctggacgc ggcccgggtt cttccggatg gtctctgcgc cttcttccgt
     1021 cagccggctc agcatgatat aggtcgccat cgctgcctcc ctcgcatcgg gattcgagcc
     1081 agtcaatctt cagcggcata gtatgcgtcc ctgcaggcgc tggctcgaag cctagcgaac
     1141 ctcacccacc gactgtgagg cggaatgcat gaacgcggtg agaattcatc agccgggcgg
     1201 gccggacgtg ctgcgctacg aagaggtgcc gacgcctcta cccgccagtg gcgaggccct
     1261 cgtacatctc cgggcgatcg gcgtgaactt catcgacatc tatcatcgca gcgggcagta
     1321 tcccgtgtcg tttcccgcca tcattggaca ggaaggcgcc ggcgtcgtcg actcggtcgg
     1381 atcgggtgtg acggacgtgt cgccgggaga ccgggtggcg tattccaatg tctttgccag
     1441 ctacgccgag tttgttacgg ttccggcctg gcggtgcgtg aagattcccg cgtccctgac
     1501 gttcgagcag gcggctgcag cgatgctgca ggggatgacg gcgcactacc tcagccacac
     1561 gacctatccg atcaagcggg gtgacatggt gctggtgcac gcggcggcgg gcggggtcgg
     1621 cgcgctgctc gtgcaaatgg ccaagcggcg gggggccaag gtcttggcga cggtttcatc
     1681 cgatgaaaag gccaaggtgg cgcgtgaagc gggggcggat gtggtcatca attactcgac
     1741 ggtggacttc gtccctgagg taaagcggct gaccgatgcg caaggcgtgc ctgccgtcta
     1801 tgactcagtg ggtcgggata cattcgaccg gagtcttgac tgcctcgcgc tgcgcgggat
     1861 gctggtcctc ttcgggcagg cgagcggccg tgttcctgcc ctcgatccac aggtcctgaa
     1921 cgcgaagggc tcggtgttcc tgacgcgccc atcgctacat cactacacgc acacccgaga
     1981 agagctattg tcgcactcga cggaagtttt gcagtgggtc ggggacggca cgatccgcct
     2041 gcgcatcgat cagagttttc cactctcacg cgcggcgaac gctcacgagt acttgcagtc
     2101 gcggcgggct atcggcaaga tcctgctcat cccgtagtcc acctcatcat gcgacctccg
     2161 gagcgtctac ccccgcccga cgctccacaa gacgtcctca cgacgtcgcg cgtccgcgcg
     2221 ctattgcggg attggatgac ggcgtctgaa gatcccgtcg tcgcgcacga tgcggtgctc
     2281 cggctcacct acggtgtgac tcttgccgtc gcggcgctgg cgctgctggc gcgacttgcc
     2341 gggatggcca tccccgcgtg gcttcttgta gcgctggccg ggacgctgct gctcgccact
     2401 cgggtccagc aactgctcct gcgtcgcggc agatatgtgc gagcgatgcc gctgctgcat
     2461 atgaatttga gccttttgct gctctcggtc gggatcgcat actcgggcgg ccttcggagt
     2521 cctttcgtgt ggacctacgc gatacccgtg gctatcgaag gtgtgctccg gggcggaccc
     2581 tgggggatca tcggcgcgac ggccagcacc atacacctgg tcggcgcgac gtttgcggcg
     2641 acatccggaa ttgcagggag gccgtttcct cctgaaccgg ggctgagcgc attcctcatc
     2701 agttacgtcg gtatgttcta tgtcgtcgcg ttcatcgcgt cctcattgcg cgagcaggtc
     2761 cgggacattt tctctttggc gcggaccgat gccctgaccg gactcgccaa tcggtacgcg
     2821 ctgcgattgt cgctcgagcg cgaggtcgcc cgggcccggc gctatggatt ggagtgcggg
     2881 ctggttgtcg tcgaaatcga tcaattcaaa cgcttcaatg accgtcacgg acatctgcac
     2941 ggcgacgaag tgctgcgccg ggtcgcggcc acgctgcggg gctcgtgccg ggccgccgat
     3001 cttcccgcgc ggtttggcgg ggacgagttt gtcgtcgtgt tgccgcagac cggtaaagga
     3061 gaggcgacgc gcctggcaga gcggattcgc ggcgacatgg aggaattcac cctgcttcgt
     3121 ggattcgggc tcacggtaag tgtgggcgtg tctgccttcc ccgacgatgg gctcaccggg
     3181 cgtgacctgc tggaagccgc ggacacggcg atgtatcgtg cgaagtccgg cggaggcaac
     3241 gcagttgccg tggccgcggg aacacattcg ggttcgtgag gcgttgaagt gattgtacgc
     3301 acgctggttg gggaaaatcg gtttggggtt ttttgcgatg cactccggac ggccggagtc
     3361 cggccactct catcaggaat cacacacacg ggcaacgcag aaaggcgatt cgcgtgcgcc
     3421 tcgctggcgc cgtgccgcgc ccaaggctgg tgtggtgctg ctcgttgccg tgctcgctgt
     3481 tggagcgctc gcgggcgtgg ccgcttcgtt ctcactggtc cccgatatct ccaaagtcta
     3541 cgacccgcca agcgaagcga cacggattta tgctgcgacc ggcgagttga ttgccagcgt
     3601 ctaccgagag aaccgggact acgtcacgct ggagcagatt ccgctcgtca tgcagcaggc
     3661 cgtcatcgcc atcgaggacg agcgattcta ccagcacagc ggtatcgacc tgcaagccat
     3721 cgcgcgtgca gcctggcgta acctgcaggc gcgcgagctg cgcgagggcg ggagcacgat
     3781 cacgcaacag ctcgcgcgtt ccgtctttct gacctcggag cggacgctga agcgaaaagt
     3841 cgccgaggcc ctactggcga ttgaaattga gcggcgcctc tccaagcgtg agatcctcga
     3901 gcggtatctc aatcaggtct actttggaca gggcgcgtac ggcatagaga tggccgcgca
     3961 ggtgtacttc agcaagcctg cctccgaact cgggcttggt gagagcgcaa tgctggccgg
     4021 actcgttcgc gcgccgtcac gatactcgcc ctatagaaac ctggagcttg cccgctacca
     4081 tcagcggttg gtcttgagga ggatggtgca actcggttac atcacgcgcg aacgggcata
     4141 cgaagcgggt gcgcgacagc cgatcacgct tgcgaggcag cggaatgtgg gcctgacaag
     4201 cattcgcgcg ccgtatttcg tgtcctacat cctgccgcat ctcctcgagc gttacggtga
     4261 ggagcaggtg tatcgcggag ggctgcaagt ctacactacg ctcgacccct cgatgcagag
     4321 cgcagcagac cgagcggtcc gggacggact cgaagaagcg cataatgcgc gtctgaacgt
     4381 ctcgcaggcg gcggtggtcg cgatcgacgc ccgctcgggt tacatccgcg cgatgatcgg
     4441 cgggtctgac tggacgcaga cccaattcaa ccgcgcgtac caggcgcgcc gccagccggg
     4501 ttcggcgttc aaacctttca tctacacgac ggccatcgag gcagggtggc gaccctggaa
     4561 gatgctgctg gacgcgccgg tcagttactg gtccggtgcg ggctactggc gtccgaagaa
     4621 ctatgacaag aagttccgtg ggccggtcac cctgcgccga gccctggaac agtccatcaa
     4681 cgtcccggcg gtgcgcacaa tgaggaagct gggccccgaa cgagtcatta cggtggcccg
     4741 gcgcatgggc atccagagcc cgctcgaacc caacctgtcg cttgcgctcg gcagctcaga
     4801 tgtcacggtg ctggagatgg cctcggccta tgcgacgctt gcgaacatgg ggatctacag
     4861 cgagccgatt gcgatccggc aggttgtgga tcagcagggg aaagtgctgg agcagaacgg
     4921 tccgcgccgt cagccggcct tggaagtgga agtcgcatat cgaatgatcg acattatgaa
     4981 aggggtcatc acccacggca ccggccgcgc ggccaggatc gggcgtcctg ccgcgggcaa
     5041 gaccgggacc tccgacgacc accgcaatgc gtggttcatc ggtttcacgc cgcacatatc
     5101 cacggccgtg tgggtcggca atgatgataa cagtcccatg cgcaaggtcg tcggcggcac
     5161 ggtgcccgcc cgcatctggg cggcattcat gcggaccgcg gtggcggcga tgcctgcgga
     5221 tgattgggac cgtcccgaag gcgtcgaggt tgaagttcgc caggccccgc gcgcggtgga
     5281 ggttccgccg ccatcgatcg aggagctgat gcggcaggcg atcatgcagc aactcaaaca
     5341 gcatctcgag cgcgccactc cggtgcgatc gaacgacccc gccgaagatg gcaccgggag
     5401 gctaaagcca cccaaggacg acggataact gcaccgcagt cctacgcttt tccggcgcgg
     5461 agtgtctgcc gcgctttttc attcccctct caacttgaca ggccggtgat cttggctcta
     5521 cgatgccaca ggctcgaggt cattggagat caaatgaacc tgtgacccac ttcttccaga
     5581 tcttctttct cgttactttt ggcagcgtgg tctcgctagc gctggggctc gtcctcgtat
     5641 ggcagcagcg gctgttccaa cgacttcatc tctatttggt cgccttcgct gcgggagcgc
     5701 tgatcgcgac ggccctcttt gatctcctcc ccgaggcgat ggaaggggtc gaggcctccg
     5761 ggacatcacc tgaaacgccg ttcatgtgga cggctgcggc aatagccatc ttcttcgtct
     5821 tcgagaagtc cttgcactgg tatcacgacc acaccggaca cgggcccgag gggaaggtca
     5881 cgaaagccgt cgtaccactg attacccttg gcgatgggct gcataatttc attgatggtg
     5941 cattgatcgc cgcagccacg ctcgcgagcc cacaactggg gttgctgact gctgtggcag
     6001 tcttccttca tgaaatccca caggaggtcg gcgacttttc agtcctcatc ttcggcggtc
     6061 tctcgagagg gacggctatt ctctacaact tgctatcggc agccgcggcg tacctcggcg
     6121 cgtttgcgac gctgattctt tcttcaggcg caacatcgtc gttaaccatg cccctcgtgg
     6181 ccctcacggc gggagccttc ctcttcatcg ccttgggcga gctccttccc gagctcattc
     6241 ataaggaaag gcgcggggcg cgaacggtcc tactgaccgc gacgttcatt tttgcgatgg
     6301 tctttatgcg ctacctcgga gagcggctcg gtgtctaagc ggtttcgccc gtgaagttcg
     6361 ccgctctact cgtctggctg ttgttctcta gcgctgcggc ggcaagcgcg caagacgtgg
     6421 agcccgagcc ggcggatgaa taccgccgcg gacgcgtcgt cgagatcgtc tttgaagagg
     6481 cacgagagct ggcaggctac cctcagttct tccagcgtgt cgtcgttgac ctgggtggga
     6541 ggcgcgtgac agcagagcac agcgagctcg ccaccgatcc tgcccggcgc cttggtgtcg
     6601 gggagcgggt cgtcgttgcg tcgacggagt cgggatactt tgtcgtcgac cgagacaggc
     6661 tgccggcgct ggcgataatc ttcggtctgt tcttcgcgat cgtcgtggcg ttcacgagat
     6721 ggcgcggcgt cgcgtcggtc tttggaatga tcgtcagcgc agtcgtcctg accaccatcg
     6781 tggtccccca gatcctggcc ggacgaagcc cgctcgcgac cagcattgtc ggcgcgctca
     6841 tcatcgcgat cacctcaatt tacctggctc atggggtcaa ccgtcgcacg tcggtggcag
     6901 tcgtgggaac gctcatcacg ctcgggctgg cgggcgcgct ctcggtcgcg tttgtttcgg
     6961 tggccaggct ctttggg
//
LOCUS       DBNGSB010000128         4196 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_857545, whole genome shotgun sequence.
ACCESSION   DBNGSB010000128 DBNGSB010000000
VERSION     DBNGSB010000128.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_857545"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="AC159B_03520"
     CDS             <1..581
                     /locus_tag="AC159B_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HIR3376852.1"
                     /translation="ARLQREVFKEVPVGLIHGRMRPEDKDAAMERFRSGGVKVLVATP
                     VIEVGIDVPQATIMVIEDADRFGLAQLHQLRGRIGRGPETSYCVLLANATTEAAEIRL
                     ASMAETRDGFLIAQRDLELRGPGEFFGTRQHGLPDLKIANVLDDGHLLERARADAKGL
                     LQSDVELKRPEHAGLREAVGRAFGRQAQPSAG"
     gene            581..1105
                     /locus_tag="AC159B_03525"
     CDS             581..1105
                     /locus_tag="AC159B_03525"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015559.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HIR3376853.1"
                     /translation="MPTDRRRRGRVRPTQQAVRDALFNSLAARVPEARVLDLFAGTGS
                     LGLEALRRGGRFAVFVEQNSALAAEIRGHLTAEGLAERAEVWRRDAVSAIRELGSTGR
                     QFDIVVADPPYGEEWITRVLRTIMTAGTLAPSGVIVAEGYWRDKPEPEPGLVCYREAK
                     YGETMLWFYRPEGG"
     gene            1108..1587
                     /gene="coaD"
                     /locus_tag="AC159B_03530"
     CDS             1108..1587
                     /gene="coaD"
                     /locus_tag="AC159B_03530"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HIR3376854.1"
                     /translation="MPIAVYPGSFDPVHLGHVDVIERAARVFDSVIVAVAKNSEKTAL
                     FTSEERLEMLRKATAHLTNVEITEYTGLTVTFARERRASVLVKGLRAVVDFEYELKQA
                     SMNQRLAPELDTVFFMTAPAYYFLSSTLIREVARLGGSVSGLVPEDIERRLRKKFEL"
     gene            1616..2137
                     /locus_tag="AC159B_03535"
     CDS             1616..2137
                     /locus_tag="AC159B_03535"
                     /inference="COORDINATES: protein motif:HMM:NF014657.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HIR3376855.1"
                     /translation="MRIDVAALEAGQVLRASLSETIDSLTEDVVFDVPVTGEVEISRD
                     PTTVRLRGMIETSTPLTCGRCLTPLRLPLRAYLNEEFLVGGEVGAEPRELKAEDFLHP
                     LGPDLILDITEVTRQQLLLALPMIPLCRPDCRGLCPQCGANWNETTCSHQVADIDPRL
                     EPLIKLRERLPEE"
     gene            2158..2355
                     /gene="rpmF"
                     /locus_tag="AC159B_03540"
     CDS             2158..2355
                     /gene="rpmF"
                     /locus_tag="AC159B_03540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HIR3376856.1"
                     /translation="MGIQKHRRSHKSGADRRGHWKIKPVALVECPKCHARMRSHRVCA
                     NCGYYAGREVIKVVEEEPKKR"
     gene            2394..3422
                     /gene="plsX"
                     /locus_tag="AC159B_03545"
     CDS             2394..3422
                     /gene="plsX"
                     /locus_tag="AC159B_03545"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HIR3376857.1"
                     /translation="MKIAVDAMGGDHAPVAVVAGAVAAARELTIPVVLVGLQADVQAQ
                     LARHRIDGLPIEVSDAPEVIQMHEPPAMALRRKRRASIVVAMEMVRDGRADAVVSAGH
                     TGAAMGAALLGIGRISGVDRPAIAAVLPTLGKTPVILLDVGANVDCKPHHLQQFALMG
                     AVYSNRVLGVSSPRIGVLSNGTEEGKGNDLTSATTELLRASPGLALVGNVEARDIFSG
                     VADVIVCDGFVGNVALKVGEGLSLAIRSIVKGELLGWRGRLLKLYVAPLVGKVRRAYR
                     RIDYREYGGAPLLGINGVCIIGHGSSNARAIRNAIRAAAEGVAHNFVQEIRTQLPALN
                     AAAAARGT"
     gene            3428..>4196
                     /locus_tag="AC159B_03550"
     CDS             3428..>4196
                     /locus_tag="AC159B_03550"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HIR3376858.1"
                     /translation="MRSSTITGLGRYIPKRIVTNAELEQLVDTSDEWIVSRTGIRERR
                     IAEPDEASSDLAYEASKEALADAGVSVEEIDLIIVGTATPDMLFPATACILQDRLGAK
                     QAGAFDVSAACTSWAYAAAVAHGYVSSGQAETVLVVGAETLSKIINWKDRNTSVLFGD
                     SAGAVVLKPCEAGKGFLSFHLGSDGSGGPFIAQPAGGSRLPASYETVEREQHYLTMNG
                     REVFKFAVRCIPRAIEEAVKLAGIDIEDVDWFIPHQAN"
BASE COUNT          794 a         1228 c         1416 g          758 t
ORIGIN      
        1 ccgccaggtt gcagcgtgaa gtgttcaagg aagtgccggt ggggctgatt cacggccgga
       61 tgcggcccga ggacaaggat gccgccatgg agcgcttcag gtccggcggc gtcaaggtgc
      121 tggtcgccac gcctgtgatc gaggtcggta tcgacgtacc tcaggccacg atcatggtca
      181 tcgaagacgc cgacaggttt ggactcgcgc agctgcatca gctccggggt cgcattggcc
      241 gaggacctga gacgtcgtat tgtgtccttc tagcgaatgc gacgaccgaa gccgccgaga
      301 tacgcctggc gtcgatggcc gagacccgtg atggattcct gatcgcacag cgcgacctcg
      361 aactgcgggg accgggcgaa ttctttggaa cccgccagca cgggctgcct gatctaaaga
      421 tcgccaacgt acttgacgac ggacatctgc tcgagcgtgc acgcgccgac gccaaaggcc
      481 tgcttcaaag tgatgtagag ctgaagcggc ccgagcacgc gggtctgcgt gaagcagtag
      541 gtcgagcatt cgggaggcag gcccaaccat ccgccggata ataccaacag atcgccggcg
      601 gcgggggcgc gtccggccca cgcagcaggc cgtcagggac gcgttattca acagcctcgc
      661 cgcgcgcgtg ccggaagcgc gggtactgga cctgttcgcg gggacgggta gccttgggct
      721 ggaagcgctg cgccgggggg ggcgttttgc cgtgttcgta gagcagaata gcgccctggc
      781 cgctgagatc cgcggccacc tgacagcaga gggtttggcc gaacgcgccg aggtctggcg
      841 gagggacgcc gtgagtgcga tccgcgagct cggcagcacc ggccgccagt ttgatatcgt
      901 ggtcgcggat ccgccgtatg gagaagaatg gatcacgcgt gtgctgcgca ccatcatgac
      961 cgcaggcacc cttgcaccgt ccggcgtgat cgtcgccgag gggtactggc gagacaagcc
     1021 cgagcctgaa cctgggctcg tctgctaccg ggaagcaaag tacggtgaga ccatgctgtg
     1081 gttctaccgc ccagaggggg gttgaccgtg ccgattgcag tgtatccggg gagtttcgac
     1141 ccggtccacc tgggacacgt cgatgtcatc gaacgggcag cccgtgtatt tgattcggtc
     1201 atcgtcgcgg tcgcgaagaa ctcggagaaa acggcgttgt ttacttccga ggaacgactc
     1261 gagatgctcc gcaaggccac cgcacatctg acaaacgtgg aaatcaccga atacaccgga
     1321 ctgactgtga cgtttgcacg ggaacgacgc gcgtcggtcc tggtcaaggg actgcgcgcc
     1381 gtcgtcgatt ttgaatacga gctcaaacag gcctcgatga atcagcggct ggcgcccgag
     1441 ctcgacacgg tgttcttcat gacggcgccc gcgtattatt tcctgagctc gacgctgatt
     1501 cgagaggtcg cgcggctcgg cggttcagtg tccggcctcg tccccgaaga cattgaacgg
     1561 cgactccgca agaagtttga gttataatga aaagtctgcg ttggggtgac tgtacgtgcg
     1621 gatcgatgtt gcggcgctgg aggccgggca ggtcctccgc gcatcgcttt ctgaaacgat
     1681 cgacagcctg accgaagacg tggtttttga cgtccccgta actggcgagg tcgaaatttc
     1741 ccgcgatcca acgaccgtcc ggctacgtgg aatgatcgag acctccacgc cgcttacgtg
     1801 cggccggtgc ctgacgccat tacggctgcc gcttcgggca tacttgaatg aggagttcct
     1861 ggtcgggggc gaggtgggcg cggagccacg ggagttgaag gctgaggatt tcctccaccc
     1921 gcttggtcca gatctcatcc tggacatcac cgaggtgacc cgccagcagt tgctgttggc
     1981 tttgccgatg attccgctgt gcagacctga ctgccgcggt ctgtgcccgc agtgcggggc
     2041 gaactggaac gagacgacgt gcagccatca ggtggcggat atcgatcctc ggctggaacc
     2101 gttgatcaaa ctgcgagaga gattgccgga agagtaagac tataaggagg atagggtatg
     2161 gggattcaga agcaccggcg ttcccacaag agcggagctg accgaagggg tcactggaag
     2221 atcaagccgg tcgcgctggt cgaatgtccg aagtgtcacg cgcggatgcg atcgcaccgg
     2281 gtctgtgcga attgcggata ttatgccggg cgggaagtca tcaaggtcgt ggaagaagag
     2341 ccaaagaagc gttaaccgca gcttttcagc tcgatcggag ctctgaacat tcgatgaaga
     2401 tcgcagtaga cgccatgggt ggcgaccacg ctcccgtagc ggtcgtcgcc ggtgctgttg
     2461 ccgccgcccg cgaactgacc atacccgttg tgctggtggg cctccaggcc gacgtgcagg
     2521 cgcagctcgc gcgccatcgc atcgatgggc tccccatcga agtgtccgac gcgcccgaag
     2581 tcatccagat gcacgagccc ccagcgatgg cgctgcggcg gaaacgccgg gcgtcgatcg
     2641 tggtggcgat ggaaatggtc cgagacggtc gcgccgatgc ggttgtcagc gccggtcata
     2701 cgggggcggc gatgggcgcg gcactcttgg gaataggacg gatctctggt gttgaccgtc
     2761 ccgcgatcgc cgcggtcctt ccgacgctgg gcaagacgcc cgtgatcctt ctggacgttg
     2821 gcgccaacgt cgattgcaag ccacatcacc tccagcagtt tgcgcttatg ggtgcggtct
     2881 attccaatcg tgttcttggg gtcagttcgc cgcgcatcgg cgtgctgagc aatggcacag
     2941 aggaaggaaa gggcaacgac ctgacctctg ccacgaccga gttgctgcgc gcgtcacccg
     3001 gcctcgcgct ggtcggcaac gtcgaggcgc gtgacatctt cagcggagtc gccgacgtga
     3061 ttgtctgcga tggctttgtt ggcaacgtcg ccctcaaagt cggcgaggga ctatccctcg
     3121 cgatccggag catcgtcaag ggcgagctgc ttggctggcg gggccggctg ctcaaactgt
     3181 acgttgcgcc gctcgtgggc aaggtccggc gcgcctaccg gcgcatcgac taccgagagt
     3241 acggtggggc gccgctgctc ggcatcaacg gggtatgcat catcgggcat ggaagcagca
     3301 acgcgcgcgc catccgcaac gcgatccgcg cagccgcaga aggtgtcgcg cacaactttg
     3361 tgcaggaaat acggacgcaa ttgcctgcgc tgaatgccgc agcggcggcc aggggtacgt
     3421 agagatcatg aggagctcga cgatcaccgg gctggggcgc tacattccca agcggattgt
     3481 gaccaatgcg gagttggagc aacttgttga tacgagcgac gaatggatcg tcagccgcac
     3541 ggggatacgt gagcgccgca ttgccgagcc cgacgaggcc agttccgatc tcgcctacga
     3601 ggcctcgaaa gaagcgctcg cggatgccgg cgtatcggtc gaagagatcg acctgatcat
     3661 cgtcggcacg gcgacgccgg acatgctgtt tccagcgacc gcctgcattc tccaggaccg
     3721 gctcggcgcc aagcaggccg gcgccttcga cgtctccgcg gcttgcacca gctgggctta
     3781 tgcggcagcg gtcgcgcatg gctacgtgag cagcggccag gccgagacgg tgctggtcgt
     3841 cggcgcagaa accctctcca agatcatcaa ctggaaagac cgcaacacca gcgtgctgtt
     3901 tggggactct gcgggcgccg ttgttctgaa gccatgcgag gcgggcaaag ggttcctgtc
     3961 gtttcacctc ggctccgacg gttccggcgg tccttttatc gcgcagccgg ccggcggatc
     4021 tcgattgccg gcgagctacg agacggtcga gcgcgagcag cattacctga cgatgaatgg
     4081 gcgagaggta ttcaaattcg ccgtgcggtg catcccgcgg gcgattgaag aggccgtcaa
     4141 gctggcgggg atcgacatcg aagatgtcga ctggttcatc ccccaccagg ccaaca
//
LOCUS       DBNGSB010000129         4818 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3158562, whole genome shotgun sequence.
ACCESSION   DBNGSB010000129 DBNGSB010000000
VERSION     DBNGSB010000129.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4818)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3158562"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1045
                     /locus_tag="AC159B_03555"
     CDS             <1..1045
                     /locus_tag="AC159B_03555"
                     /inference="COORDINATES: protein motif:HMM:NF017745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HIR3376859.1"
                     /translation="ANASDDYACGTEALDLLLRCGHFLDMSSSAVLAFAQEHFSAAES
                     RLRESATRLGIADWREGLRELADLHPPTKSYYARFTEVWLEAKAAAEAHGLVTWPDYP
                     LRYVPQPSWARDAAPYLYFLFYRAPAAFDDLPVVDYLVTPVEPEMDPEEQTRRLRATN
                     ESVIKLNHVIHHGGLGHHIQNWHAYRTASRVGQIAAVDCASRIAMFCGGTMAEGWACY
                     ATDLMDEAGFLTPLEHLSHAHNRLRMAARAMADVQLHTGAWTLEKTVIFYRDSAGIPV
                     DAARAETVKNSMFPGVALTYLCGTELIRQLRREMAARGRGFDLRRFHDKFLSYGSVPV
                     ALIAAEMRREISH"
     gene            complement(1042..2064)
                     /locus_tag="AC159B_03560"
     CDS             complement(1042..2064)
                     /locus_tag="AC159B_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015620805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HIR3376860.1"
                     /translation="MIDVLIASYLEPNFVDQIRAIDRARVTYEPALLPQPRYLCDHVG
                     HPLQRTSQDEQRWRGHLAGAEVLFDFDYTNIKILRTLIPKVRWIQGSSTGIGQLLVRT
                     GLIDSPIAFTTARGIHAKPLADFVLMAILWFAKDGFRMVRDQAAHRWVRYCGRDVRGA
                     TVGIVGFGTIGREVARACRALGMRVIATKRTAVAGDHDADADTLVPVGGLGSLLGVAD
                     YVVLAIPQTPGTERILGQKQLTAMKRGAVLINVARGAVVDEDALISSLRDGHLGGAAL
                     DVLATEPPAADNPLWNMPNVLISPHSASTVASENAVLTELFCDNLLRYMNGEPLLNMF
                     DRERLY"
     gene            2191..2865
                     /locus_tag="AC159B_03565"
     CDS             2191..2865
                     /locus_tag="AC159B_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR3376861.1"
                     /translation="MAQTKKREFRDWDYWGRPVPGFGDPNARLLVIGLAPAAHGANRT
                     GRMFTGDSSGQWLIRAMYRAGFASQPTTTHRGDDLRLMNSYLTAIVRCAPPDNRPVPA
                     EISNCAGYLRGELTLLKRVRVVVTLGQMAFHQYLSLAKERGALVPRPRPPFRHGATYT
                     FSSKVEPTQPVLVASYHPSRQNTQTGRLTEVMLDEIFSRVRDLLKRVPSRRPVPERGA
                     SGTGHA"
     gene            2858..4312
                     /locus_tag="AC159B_03570"
     CDS             2858..4312
                     /locus_tag="AC159B_03570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR3376862.1"
                     /translation="MRDLPLRPVFIALLALTAIRILAASAYPLVDDEAYYWTWSRHLA
                     WGYPDHPPAVAAMVKLTTALVGDSAVGIRLGPISLALLSSLLIYDLGRRMFERRTALL
                     AAIGYQIIPVVAVGAIFTFPDAPFIFFWILAVWCIWRARELGRVWDWYAAGLAVGLAF
                     LSKLSALFLAVSLAGFFLTFEADRRWLRRPEPYRAAAIALAVVFPFFAWNSRHDWITI
                     AKTLHPIPWIDLGGPAKNAAAFLGATAAYYGPIALGLLVAALLASIRRARASDHRFAL
                     LSWLALPLIGFTFISSFDGIPKPHWPAPAYLMLVIAAVALWPSVSQRRWWRRTVIVGV
                     AVNTVLVAALHVVPFLPSSLTAQHLLGWDQAAATVAGLIERRTPPGPFVLTGSYQNAS
                     QLTYHLRERYPITTPRQDNEIAIRLDPVSMLDRDAIFVTDHAPGPGLPLHLMFKRVQR
                     RPDVEVRIGSGVRRFQVYIGYGFRGLPRARFSPI"
     gene            complement(4388..4741)
                     /locus_tag="AC159B_03575"
     CDS             complement(4388..4741)
                     /locus_tag="AC159B_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HIR3376863.1"
                     /translation="MAKTYAKWGGIILLVLGVVGLVVGDSFLGLNTEFIEDVIHIVAG
                     GLLLYAGTRGTDAQAVSWARIFGIVFLIVGVVGFFSKNLFGLLPAVGLSVLDNIVHLA
                     YGVVGLLAARGYKMA"
BASE COUNT          850 a         1570 c         1436 g          962 t
ORIGIN      
        1 cgcgaatgcc agtgacgatt acgcgtgcgg gaccgaagca ctcgatctgc tgctccgctg
       61 cggtcacttt cttgacatga gtagttccgc cgtgctcgca ttcgcccagg agcacttcag
      121 cgcggcggaa tctcgtctga gggagagcgc tacacgcctg gggatcgcgg actggcggga
      181 gggactgcga gagttggccg acctccatcc gccgacaaaa tcgtactacg cacgtttcac
      241 cgaggtctgg ctggaagcga aagccgcagc ggaagcgcac ggacttgtaa cgtggccgga
      301 ttatcccctt cggtatgtgc cgcagccgtc atgggcgcgg gatgccgcac cgtatctcta
      361 cttcttgttc tatcgtgccc cggccgcgtt tgatgacctt ccggtggtgg actatctcgt
      421 gacgcccgtc gagcccgaga tggatcccga ggaacagacc cgccgcctcc gggcgacaaa
      481 cgagagcgtg atcaagctca accatgtgat ccatcacggg ggcctgggtc accacatcca
      541 gaactggcac gcgtaccgca cggcctcacg cgtcggccaa atcgcggccg tggactgcgc
      601 cagccgcatc gcgatgttct gcggcggcac catggccgag gggtgggcat gctatgcgac
      661 agatctcatg gatgaagccg gatttctcac gccactcgag catctctccc atgcgcacaa
      721 ccggctgcgg atggcggcca gggcaatggc cgatgtgcag ctgcacacgg gcgcatggac
      781 gcttgaaaaa accgtcatct tttaccgtga tagtgcgggg atccctgtcg atgcggcacg
      841 cgccgagacc gtgaagaaca gcatgttccc cggcgtggcg ctcacatacc tttgcggtac
      901 tgagttgatc cgccagctgc ggcgagagat ggcggcgcgc gggaggggct ttgatctgcg
      961 ccgcttccac gacaagtttc tgtcttacgg atcagtaccc gtggcgctca ttgcagcaga
     1021 gatgcgccgt gagatctcac actagtacaa ccgttctctg tcgaacatgt tcaaaagtgg
     1081 ttcgccattc atgtagcgga gcagattgtc gcagaacagc tcggtcagaa cggcgttctc
     1141 ggacgccacc gtgctggcgg agtgcgggct gatcagcaca tttggcatgt tccacaatgg
     1201 gttgtccgct gccggtggct ctgtcgccaa tacgtccaga gccgcaccac ctaagtgccc
     1261 gtcgcggagc gacgaaatga gggcgtcttc gtccaccacg gcgccgcggg ccacgttgat
     1321 cagcaccgcg ccgcgcttca tggcggtcag ttgtttctgg cccaagatcc tctcggtccc
     1381 cggagtctgc ggaattgcca gcaccacgta atccgcgacg cccagcagcg atcccagacc
     1441 gcccactggc accagcgtgt cagcatcggc gtcgtgatcg cctgcaacgg ccgtgcgctt
     1501 cgtggcgatg acgcgcatac ccagagcccg gcacgctctt gccacctcac gtccgatggt
     1561 gccaaacccc acgatgccca ccgtcgcgcc gcgcacgtcg cgaccgcagt agcgaaccca
     1621 ccggtgggcc gcctgatcgc gcaccatgcg gaatccgtcc tttgcaaacc ataaaatcgc
     1681 catcagtacg aagtcggcca gcggtttggc gtggatacct cgggcggtcg taaacgcaat
     1741 tggactgtca atcagcccgg tgcggaccag gagctggccg attcccgtgc tcgatccctg
     1801 gatccatcgc acctttggga tgagggtcct taggatcttg atgttggtat agtcaaagtc
     1861 gaagagcact tccgcaccgg cgagatggcc ccgccagcgt tgttcgtcct ggctggtgcg
     1921 ctgcagggga tgcccgacat gatcgcacag ataccgcggc tgtggcagca aggccggctc
     1981 gtaggtcacc cgcgcccggt cgatggcgcg gatctggtcg acgaagttcg gctcaaggta
     2041 tgaagcgatc agtacatcga tcacttagtc ctcctcgctg gcacccatgg taccatctgc
     2101 tgtgtgccga ccgtttccaa cctgcgacga ctcgcgcgga cgatcgtcgt ctgtcacaga
     2161 tgcccgcgac ttgtccatta tcgggagcat gtggcccaaa cgaagaagcg cgagtttcgt
     2221 gactgggatt attggggccg gccggttccc ggatttggcg atccgaacgc gcgcctcctt
     2281 gtcatcggcc tcgcgccggc cgcgcatggg gcaaaccgga cagggcggat gtttaccggt
     2341 gacagttctg ggcagtggct gattcgtgcg atgtaccgcg cggggttcgc gtcacaaccc
     2401 acgaccacgc accggggtga cgatctgcgc ctgatgaact cgtatctgac ggcgatcgtc
     2461 aggtgcgcgc ccccggacaa cagaccagtg ccggcggaaa tctcaaactg cgccggttac
     2521 ctgcgcgggg aactcaccct gctcaaacgg gtgcgcgtgg tcgtgacgct cggacaaatg
     2581 gcgtttcacc aatacctgtc cctcgccaaa gagcgcggcg ccctcgtccc gcggccgcgg
     2641 ccgccattcc ggcacggcgc aacgtacacg ttctcgtcga aggtcgagcc cacgcagccg
     2701 gtcctggtcg cgtcgtacca ccccagccgg cagaacaccc agacgggacg cctgaccgag
     2761 gtgatgctcg acgagatctt cagccgggtc cgcgatctcc tcaagcgggt cccgtcgcgc
     2821 cggcctgtac ccgagcgcgg ggcgagtggt acagggcatg cgtgatctgc cccttcgacc
     2881 cgttttcatc gccctgctcg ccctgaccgc gatcaggatt ctcgctgcat cagcatatcc
     2941 gctcgttgac gacgaagcct attactggac ttggtcgagg catctggcgt ggggatatcc
     3001 ggatcatccc ccggcggtgg ccgcgatggt caagctcacg acggccctgg tcggtgactc
     3061 cgccgtcggc atccggctgg gtcctatttc cctcgcgttg ctctcgtcgc ttctgatcta
     3121 cgatctcggc cggcgaatgt ttgagcgtcg cacggcctta ctcgctgcta ttggatatca
     3181 aatcattcct gtcgtcgccg tcggcgccat cttcaccttt ccggatgcgc cgttcatctt
     3241 cttctggatc ctggcggtgt ggtgcatctg gcgcgccagg gaactcggcc gcgtgtggga
     3301 ctggtatgcc gcagggctgg cggtaggact cgcattcctc agtaagctct ccgcgttgtt
     3361 tcttgcggta tcgctcgcgg ggttctttct gacgttcgaa gccgaccggc gctggctccg
     3421 gaggcccgag ccgtaccgcg ccgcggcgat cgcactggcg gttgtgttcc ccttcttcgc
     3481 ctggaacagc cgccacgatt ggatcacgat cgccaagacg ctccacccga ttccgtggat
     3541 tgatctcggt gggccggcga agaatgcggc cgcctttctc ggcgctacgg cagcatacta
     3601 cgggccgatt gctcttggat tgctggttgc cgccctcctg gcgagcatcc ggcgcgcgcg
     3661 cgcgtctgac caccgctttg cattgctcag ctggcttgcg ctcccgctta ttggtttcac
     3721 gttcatcagc agcttcgacg gcattccaaa accgcactgg cctgcgcctg cctatctcat
     3781 gctcgtcatc gctgctgtcg ccttgtggcc gtccgttagc cagcgccgct ggtggcggcg
     3841 cactgtcatc gtcggcgtcg cggtgaatac cgtcctggtg gctgcgctgc acgtggtccc
     3901 gttcttgccc tcctcgctca cagcgcagca tctgctgggg tgggatcagg ctgcggcaac
     3961 ggttgccgga ctcattgaac gacgcacccc tcccggcccg tttgtcctga cgggaagcta
     4021 tcagaacgcc agccagctca cctaccacct gcgcgagcga taccctatca ccaccccgcg
     4081 ccaggacaat gagatcgcga tccgcctcga tcccgtgtca atgctggatc gtgatgccat
     4141 ctttgtcacc gaccacgctc ccggaccagg cctgccgctc catttgatgt tcaaacgcgt
     4201 gcagcgccgc ccagatgtcg aggtccgcat tggttcgggc gtgaggcgat tccaggtata
     4261 tatcggttac ggtttccgcg gtcttccccg agcgagattc tctccaatct agtcgcacca
     4321 cgcacgactt gaaaggaccc gggcgcttcg cccggatcct tttgaggcct aaggacaatc
     4381 cggaggatta tgccatcttg tacccacgag ccgccagaag accgaccact ccgtacgcca
     4441 ggtggacaat gttgtcgagg acgctcaggc caacggccgg gagcagtcca aacagattct
     4501 tggaaaagaa cccgacgacg cccactataa ggaagacgat cccgaagatc ctcgcccacg
     4561 acacggcctg ggcatcggtg cccctggttc cggcgtagag gaggaggcct ccggcaacga
     4621 tgtgaatcac atcctcgatg aactcggtgt tcaatcccaa aaatgagtcc ccaacgacca
     4681 ggccgacaac acctaagacc aagaggatga tcccgcccca cttcgcgtag gttttggcca
     4741 tcgatttccc ccctcgtgta tggattacaa gtctcttatc gtcagtagca gattctcctt
     4801 ccgcaacgtc acattgga
//
LOCUS       DBNGSB010000130         4221 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_553862, whole genome shotgun sequence.
ACCESSION   DBNGSB010000130 DBNGSB010000000
VERSION     DBNGSB010000130.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4221)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_553862"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /gene="lpxD"
                     /locus_tag="AC159B_03580"
     CDS             complement(<1..809)
                     /gene="lpxD"
                     /locus_tag="AC159B_03580"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HIR3376864.1"
                     /translation="MAAAIGGEAVGDREVEIVGVAEIASAGPGRIVMAADARRLAEAE
                     GSTASAVLLPENLNSERKPAVRAKNIRLAFGRAIGLLHPLRRPEPGIHPTAIVGEGTR
                     VESSVSIGPYAVIGEECEIEDHVTIGAACAIGDGVRIGEESILYPHVTVYARCIIGAR
                     VILHSGAVLGSDGFGYAAVNGRQVKIPHVGRVVIEDDVEIGANSAIDRATLGETRIGA
                     GTKIDNLVQIGHNVVIGRDVVIAGQCGVSGSVTIGDRAVLAGKVGVVDHAI"
     gene            complement(858..1271)
                     /locus_tag="AC159B_03585"
     CDS             complement(858..1271)
                     /locus_tag="AC159B_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH/Skp family outer membrane protein"
                     /protein_id="HIR3376865.1"
                     /translation="MTSVGRVMLGLLAAATLVISVGCGGPRIGVVDSQRILDESPLAL
                     TYQRELDSREKAMAEDLRLLVGRMAAGDLEARRQIYLRELSQRKGELEGKLNERIRKE
                     VAEIARHQRLRFVLVKGATQLGGVDITQSVIDRLK"
     gene            complement(1268..2377)
                     /locus_tag="AC159B_03590"
     CDS             complement(1268..2377)
                     /locus_tag="AC159B_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376866.1"
                     /translation="MRVLLLVLAIPLIAGGCRNRQASPPPPQAAETREPTVSRIGVVD
                     IAAVARAHPRWSEIEALNKKIQRSEVEIAGAPPLPPPPVPDLRPALDAEGLQLRAEFD
                     KELAAMQEDLRRQLEAFAASLREQQEVRFVALQQQLHEEGKKAIEAQHAELDKQLKAD
                     ELAIMNEYKYPMLNLRLRAEVAGLSSEQEGREVVRQIQALQQEREERLRTKKEEVTKT
                     AEEFAKVKEDELNARLEAAQDALGEEGKQQLSAREQQAQAQLKTVLAQREKAFQERLE
                     ERRRELIRTAEEQLRGRQGAYLNDLSARARQARAELVALQEQRARLEDSIMAEVKIEV
                     AAIAQQERLDVVLTRLISSLGGIDITQKVVQKFKR"
     gene            complement(2383..2850)
                     /locus_tag="AC159B_03595"
     CDS             complement(2383..2850)
                     /locus_tag="AC159B_03595"
                     /inference="COORDINATES: protein motif:HMM:NF015871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH/Skp family outer membrane protein"
                     /protein_id="HIR3376867.1"
                     /translation="MQIQRRRLPTVWLAGGLIAVVAVGLVAVLAFSPGGPVLGQSFTI
                     GYVDMARAVGSHPRRQSSERALQEFFQAKQREFQQRTKSMNAVQRQELDRQLQQQFIG
                     KREELIGGLDKDIRGAVEKVAQERGVGIVLDRNVVLYGGTDLTDAVIAKLSGK"
     gene            complement(2958..>4221)
                     /locus_tag="AC159B_03600"
     CDS             complement(2958..>4221)
                     /locus_tag="AC159B_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376868.1"
                     /translation="ILLLACIVLGAGLPALAQATVQRVDVRVTFDEGAPHPLFAERLQ
                     AAAQSVVERLLVGRPIDQLQALQPRLQETVAAVLDRVATGYAVSGTIAQLGATSTVTL
                     SLRRVGPVIGDATVRPDLTIFHPRIRPLAEGALQARAGEIRALVTNLPIAALEWAEPI
                     AAGRAREIVEEALPGFSTAVRISAGVSATVELIVNTRDSRIVRNIGVRFRSTSIPTLL
                     LDRDAPQVISIAEPLRGLPVVFAEAQRGPLARLIDEDLVKSPPVRQYRIVTTVGLDVG
                     ETTYVNVAADSLLYRARVEAHLNVGPRAPNPVLVAHLGRLVHPSTELFVEMRLTPNTL
                     SMDWDVGAQYQLSTDAAIGLSYTVGAQSVAAWTIVRFGRDVSTRGKWNLTQQAFEGAL
                     TYRVNEFLAGEIIATSLGEVWLRLISNL"
BASE COUNT          720 a         1425 c         1256 g          820 t
ORIGIN      
        1 atgatcgcgt gatccacgac gccgacctta ccggccagga ccgcccgatc gccgatcgtg
       61 acactgccgg agaccccgca ctgccccgcg atgacgacgt cccgtccgat cacgacgttg
      121 tggccgatct gcaccaggtt gtcgatcttc gtgcccgccc cgatacgagt ctcaccgagt
      181 gtcgcccggt cgatcgctga gtttgctccg atctccacat cgtcttcgat cacgacgcgg
      241 ccaacatggg gaatcttcac ctgccggccg ttgaccgctg catacccaaa cccatcgctt
      301 cccagcacgg cgccgctgtg cagaatcacc cgcgccccga tgatgcagcg cgcgtataca
      361 gtgacatggg gatagaggat cgactcttcg ccgatccgga cgccgtcacc gatcgcgcac
      421 gcggcgccga ttgtgacgtg atcctcgatc tcgcactcct caccgatgac ggcataggga
      481 ccgatggaga cagaactttc gacccgcgtc ccttcgccca cgatggccgt gggatgaatg
      541 cctggctcgg gacgacgcag cggatgaagg aggccgatgg ctcgcccgaa ggccagcctg
      601 atgttcttcg cgcggaccgc gggctttcgc tcactgttga ggttctcggg aagcagaacg
      661 gcggaggctg tgctgccctc cgcctcggca agacggcgcg cgtccgccgc catcacgatc
      721 cttcccggcc cggcgctcgc gatctcggct acgccgacga tctcgacctc acgatcgccg
      781 accgcctctc cgcctatcgc cgccgccaat tcatgtagtt tcacacaaac gagtcccttc
      841 gtgtagcgcg ttaccgtcta tttcagccgg tcaatcacac tctgcgtaat gtccacgccg
      901 cccagttggg tggcgccctt caccagcacg aagcgcagtc gctggtggcg tgcgatttcc
      961 gcgacttcct tgcggatccg ttcgttcagc ttcccttcaa gctcgccttt gcgctgtgag
     1021 agttctcgga gatagatttg ccggcgtgct tccaggtccc cagccgccat gcgcccgacc
     1081 aggagccgca ggtcttcggc catcgccttc tcgcgactgt cgagctctcg ctggtacgtc
     1141 agcgccagcg ggctttcatc gaggattctt tgggaatcca cgacaccgat gcgcggcccg
     1201 ccgcaaccaa cagaaatcac caaggtggcc gcggcaagca ggccaagcat gacgcgaccg
     1261 actgaggtca tcgcttgaat ttctggacga ctttctgggt gatgtcgatg ccgccaaggc
     1321 tgctgatcaa tcgcgtcagg accacgtcca atcgctcctg ttgagcgatg gcggcgactt
     1381 cgattttcac ttcggccatg atgctgtctt ccaggcgggc ccgctgttcc tggagagcca
     1441 ccagttcggc ccgcgcctgc cgcgcgcgcg ccgagaggtc gttcaggtat gcgccctgcc
     1501 gtcctcgcag ctgctcctcc gccgtgcgga tcagctcacg tcggcgttct tccagtcgtt
     1561 cctggaatgc tttctcccgc tgggcgagga cggtcttcag ctgcgcctgg gcctgttgct
     1621 ctcttgccga cagttgctgc ttgccctctt cgcccagggc gtcttgagcg gcctcgaggc
     1681 gggcgttgag ttcgtcttcc ttcacttttg caaattcttc agcggttttg gtgacttctt
     1741 ctttcttggt gcgcagccgc tcctcgcgtt cttgctgcag cgcctgtatt tgtcgaacca
     1801 cctcgcggcc ttcctgctcg gagctcaatc ccgcaacctc agcccgcagc cggaggttga
     1861 gcatggggta cttgtactca ttcatgatcg ccagctcgtc ggctttcagc tgtttgtcga
     1921 gctccgcgtg ctgcgcttcg atggccttct tgccttcctc gtgaagctgt tgttgtagtg
     1981 cgacgaaccg cacctcctgc tgctcgcgca gcgacgcggc gaacgcttcc aactggcgcc
     2041 ggagatcttc ctgcattgcg gccagctctt tgtcgaactc cgcgcgcagc tgcaatcctt
     2101 cggcgtcaag cgcggggcgc agatccggca ccggcggtgg gggcagcggc ggggccccgg
     2161 cgatctcaac ttcggaccgc tgaattttct tgttgagggc ttcgatctca gaccacctgg
     2221 ggtgtgcgcg cgcaacggcg gcgatgtcca ccacgccgat gcgggagacc gtgggttctc
     2281 tcgtctcagc ggcctgtggt gggggcgggg aggcttggcg gttgcggcat cctccggcga
     2341 tcaacgggat ggccagaacc aacaggagaa cgcgcaaggg ggttatttcc cgctcagttt
     2401 tgcgatcact gcatcggtga gatccgtgcc gccatacagc acgacgttgc ggtccagcac
     2461 gatgccgacc ccgcgttctt gagcgacctt ttcgaccgcg ccgcgaatat ctttatccag
     2521 cccgccgatc aactcttcgc gtttgccgat gaactgttgc tggagctggc gatcgagttc
     2581 ctggcgctgc accgcattca tgctcttcgt gcgctgctgg aactctcgct gcttcgcctg
     2641 aaagaactcc tgaagggcac gttcagacga ctgacgccgc ggatgcgatc cgacggcgcg
     2701 ggccatgtcg acgtatccga tcgtaaatga ctgcccgagt accggtccac cgggcgagaa
     2761 cgccagcacc gcaaccagcc caaccgcgac caccgcgatc aacccaccgg ccagccagac
     2821 cgtcggcagt cgacgtctct gaatctgcat cgtcatcctc caggcaaaga ctttcctagt
     2881 gtaacgccac actccctata atacgagacg cacagacgct gccaagcgtt cacttcctgg
     2941 gctcatgtta ccgtccttta caggttgctg atcaaccgga gccatacttc tccgagcgaa
     3001 gtggcgatga tttcgccggc gaggaactcg tttacccggt acgtcagcgc tccttcaaat
     3061 gcctgctgtg tgagattcca cttcccgcgc gtgctcacgt ctcggccaaa acggacgatg
     3121 gtccaggccg caacggattg cgcccctacc gtgtagctca ggccaatggc agcgtcggtg
     3181 gacagctgat actgggcgcc cacgtcccag tccatcgaca gggtgtttgg ggtgagacgc
     3241 atctcgacga acagctccgt ggacggatgg accagccggc cgaggtgcgc gaccagcacc
     3301 ggattcgggg cacgcggccc gacgttcaga tgcgcttcca cgcgcgcccg gtacagcaga
     3361 gagtccgcgg ccacgttgac gtaggtcgtc tcccccacgt cgagccccac ggtcgtgacg
     3421 atccggtact gccgcaccgg aggcgacttg accagatcct catcgatcag acgcgccagc
     3481 gggccgcgct gcgcctcggc gaagaccaca ggcagccctc gaagcggttc cgcaatcgag
     3541 atcacctgag gcgcatcccg atccagcaac agcgttggaa tggatgtcga gcggaagcga
     3601 acgccgatat tgcgaacgat cctcgaatcg cgagtattca caatcaactc aacggtcgcg
     3661 gatacgccgg cggagatccg aacggccgta gaaaaccccg gcagcgcctc ctcgacgatc
     3721 tcacgcgcac gcccggcggc gattggctcc gcccactcca gcgcggcgat gggaaggttt
     3781 gtgacgagcg cccggatctc gccggcgcgt gcctggagtg caccttctgc aagcggacgg
     3841 attcgtgggt ggaagatcgt aaggtcgggt cgaaccgtcg catccccaat cacgggtccc
     3901 acccggcgca gactgagggt cacggtcgac gtggcaccca gttgtgcgat cgttccggac
     3961 accgcatagc cggtggcgac gcggtcgagc accgcggcga ccgtttcctg gagtctcggc
     4021 tgcaacgcct gcaactgatc gatcggacga cccaccagca gccgctcgac gacgctctgc
     4081 gctgcagcct gcaggcgctc ggcaaaaagc gggtgcggcg cgccctcgtc gaaggtcacg
     4141 cggacgtcaa cgcgctgcac tgtcgcctgg gcaagcgcag gcagcccggc gccaagcacg
     4201 atgcaagcga gcaagagaat t
//
LOCUS       DBNGSB010000131         3237 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_1013581, whole genome shotgun sequence.
ACCESSION   DBNGSB010000131 DBNGSB010000000
VERSION     DBNGSB010000131.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_1013581"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="AC159B_03605"
     CDS             <1..336
                     /locus_tag="AC159B_03605"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR3376869.1"
                     /translation="YKTLAFAVSAAYAGIGGAMLAFILRYLEPQLFSLFESIYYFSMI
                     VIGGLGTVPGSVLGAAVLTVLPQQLAGLKEWLPVVYGAAIMFMMAVEPHGLYGRWLRI
                     RLWFKTWPF"
     gene            324..1202
                     /locus_tag="AC159B_03610"
     CDS             324..1202
                     /locus_tag="AC159B_03610"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR3376870.1"
                     /translation="MALLAQYLVGGLATGSLYALVALGIVLLYRSSRVLNFAHGDLAT
                     FATFVAFSLLTAFHLPFTISSAGSLVAAAATGAAFYFLILRPVRDSTLLGTIVITLGL
                     ALVVNGAVVQIWGNETKVFPFPLSDTKVYRVGSVVISEVSIGSVLAGLVLMGLLYVLV
                     QRTKVGLAMRAVSQNLVAAQALGIPAKSIFALTWGLASALGAAAGILLAPVTFLDPFM
                     MVDPFLKGFAAAVLGGIDSMPGAIFGGLLLGVAESLFGGYVSVKFKTTLAFLIIILVL
                     VVRPEGMLGREYKRRV"
     gene            1291..2475
                     /locus_tag="AC159B_03615"
     CDS             1291..2475
                     /locus_tag="AC159B_03615"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3376871.1"
                     /translation="MKIAGRLVSVCLLLAVLATATLAQTQERGVTATEVVIGTSQPLS
                     GPAAFWGVPITGAMDAWIKFINDQGGIHGRKIRLVSRDDSYLPPRAVANARELVDRER
                     IFAMVGLLGSANSFAVRDFVVENQVIWVNPLADANMWAGFRQTKHLFVTYPSYTDEGR
                     ILADYAAKNMGVKTMAVFYQNDLYGQKGVLGVKRGAAQAKAKVVASVSYEVTDTDVSG
                     HALKLRQANPDAVILYATPRHGALIVREMVKIGFQPKLLSSFTLTDPAMFQLAGDAWN
                     NVVLTAYFPLPGSDPKVDEVLATLTRINPALAQNPFNAVAGVAFLEPFLEGMRRAGPN
                     LTRDSFVAAMESIRNWDGMVVRGVTFGPNKHQGTNRIYIVRAENRQFRKLTDWISYPT
                     GF"
     gene            2531..>3237
                     /locus_tag="AC159B_03620"
     CDS             2531..>3237
                     /locus_tag="AC159B_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006420991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3376872.1"
                     /translation="MVALGGVSLRVPSGALISIIGPNGAGKTTLFNCICGLYRADSGL
                     IRFSGTPLGGLKPDAVARRGIARTFQNIELFLRMTVLENLLLGRHLHFRTGFMAASLG
                     TSQWRHQEVQHREVAERILDFLDLQAVRDRRVGDLPLGRQRMVELGRALATEPTLLLL
                     DEPSSGMTAEEKEDLVFRIRDIRDEMAATIILVEHDLKLVMGISDWIAVLDHGEKIAE
                     GTPEEIQVHPDVIRAYLG"
BASE COUNT          584 a          935 c         1023 g          695 t
ORIGIN      
        1 tacaagacgc tcgccttcgc ggtcagcgcc gcgtacgcgg ggatcggggg cgccatgctg
       61 gccttcatcc tccgctacct ggagccccaa ctattctcgt tgtttgaatc catctactac
      121 ttcagcatga tcgtcatcgg agggctgggc acggtccctg gatctgtgct cggggcggcg
      181 gtcctgacgg tgctgccaca acaactggcc gggttgaagg agtggctgcc ggtcgtatac
      241 ggcgccgcga ttatgttcat gatggcggtc gagccgcacg gactgtacgg acggtggctg
      301 cggatcaggc tgtggttcaa gacgtggccc ttctagcgca gtacttagta ggtgggcttg
      361 ccacgggaag tctgtatgcc ctcgtggcgc tcggtattgt gctgctttac cgcagctcgc
      421 gcgtgctaaa tttcgcgcac ggcgatctgg cgacgtttgc cacgttcgtt gcgttctcgc
      481 tcctcactgc gtttcatctc cctttcacga tatcgagtgc ggggagcttg gtcgcggccg
      541 ccgcgacagg ggcggcgttc tatttcctca tactccggcc cgtccgcgac tcgacgctgc
      601 tgggaacgat cgtcatcaca cttggcctgg cgctggtggt caacggagcg gtggtgcaaa
      661 tctggggcaa cgaaacaaaa gtcttcccgt ttccactctc ggatacgaag gtgtaccgag
      721 tcggcagtgt ggtcatcagc gaggtcagca tcgggagtgt gctggccggt ctggtgctga
      781 tgggactgct ttatgtcctc gtgcaacgga cgaaagtggg gctcgccatg cgggccgttt
      841 cgcagaatct ggtcgcggcg caggctctcg ggattccggc gaaaagcatc tttgcgttga
      901 cttggggcct ggcgtcggca ttgggagccg ccgccggcat tctgctggcg ccggtcacgt
      961 ttctcgaccc tttcatgatg gtggacccgt tcctaaaagg cttcgccgcc gcggtgctgg
     1021 gtgggatcga cagcatgccg ggggcgatat ttggaggatt gctgcttggg gtggccgagt
     1081 cgctgtttgg cggctatgtg tcggtaaagt tcaagaccac gctggcgttc ctgatcatca
     1141 ttttggtcct ggtcgtgaga ccggagggaa tgctcggccg cgagtataag cggcgcgtct
     1201 gacgcttgct tcaaggaagg taagaaggtg cgttgcgagg tagaggtatg gcgtcccatc
     1261 tgcattgttg aaacttagag gggtggagca atgaaaatcg caggccgctt ggtcagtgtt
     1321 tgtctgctgc tggcagtatt ggcgacggcc acactggcgc agacccagga acgtggtgtg
     1381 acggccaccg aggtcgtgat cggcacaagc caaccgctgt cgggcccggc cgcgttctgg
     1441 ggcgttccca tcaccggtgc aatggatgcc tggatcaagt tcatcaacga ccagggcggc
     1501 atccatggcc gcaagattcg tcttgtgtcg cgcgacgatt cttatctgcc tccgcgcgct
     1561 gtcgcgaacg cccgcgagct ggtggaccgt gagcgaattt tcgcgatggt cgggctcctc
     1621 ggttcagcaa attcctttgc ggtgcgcgac ttcgtggtcg agaaccaggt catctgggta
     1681 aatccgctgg ccgacgcaaa catgtgggcc ggctttagac agacaaagca cctgttcgtc
     1741 acctacccga gctacaccga cgagggccgc atcctcgccg attacgccgc caagaatatg
     1801 ggcgtgaaga ccatggcggt cttctaccaa aatgaccttt acgggcagaa gggcgtgctc
     1861 ggggtgaaac gcggcgccgc gcaggcaaaa gccaaagtcg tggcttcagt gtcctacgag
     1921 gtcaccgata ccgacgtcag cggacatgcc ctgaaacttc gccaggcaaa tcctgatgcg
     1981 gtgatcctct acgcgacgcc gcgacacggc gcgcttatcg tccgggagat ggtcaagatc
     2041 ggcttccagc ccaaactcct cagcagtttt accctcaccg atcccgcgat gtttcagctg
     2101 gccggtgatg cctggaacaa cgtcgtattg acggcgtact tccctcttcc cgggagcgac
     2161 ccgaaagtcg acgaggtgtt ggccactcta acccggatca atcccgcgct ggctcaaaac
     2221 cccttcaacg cggtcgccgg cgtggcgttc ctcgagccgt tcctcgaagg catgcgcagg
     2281 gccggaccca atttgactcg tgacagcttc gtcgccgcaa tggagtcgat ccgaaactgg
     2341 gacggcatgg tcgttcgtgg cgtaacgttc ggtcccaaca aacaccaggg gacaaacagg
     2401 atctacattg tcagagctga aaaccgccag ttcagaaagc tgactgactg gatctcgtat
     2461 ccaacgggat tctaaggttg tccgaggcga gcttctcgcc gtcgacgggt taagcatcgc
     2521 cttcggcgga gtggtcgcgc tgggcggcgt gagtctgagg gtgccgtccg gcgcgctcat
     2581 ctcgatcatc ggccccaacg gcgcggggaa gacgacactc ttcaattgta tctgcgggct
     2641 ctatcgggct gattcggggc tgattcgatt ctcaggtacg ccccttggcg ggttgaaacc
     2701 cgatgcagtc gcccgccgcg gaatcgctcg aacgtttcag aacatcgagc ttttcctgcg
     2761 tatgacggtg ctggagaatc tgctgctcgg ccggcacctg cacttccgca ccggattcat
     2821 ggccgcctcc ttgggaactt cgcaatggcg ccaccaggag gtccagcacc gcgaggtggc
     2881 cgagcggatt ctcgactttc tcgacctgca ggcggtgcgc gatcgccggg tcggagatct
     2941 cccgctggga cggcagcgga tggtcgagct tggacgggcg cttgcgaccg aacccaccct
     3001 acttctcctg gacgaaccgt cgtctggaat gaccgcagag gagaaggaag acctcgtgtt
     3061 ccggatccga gacattcgcg atgaaatggc tgcgaccatt atcctcgtcg agcatgatct
     3121 caaactcgtc atgggaatct ccgactggat cgcggttctc gatcacggcg agaagattgc
     3181 tgaaggcacg cctgaagaga ttcaggttca tcctgatgta atccgagcct atctcgg
//
LOCUS       DBNGSB010000132         4169 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3774144, whole genome shotgun sequence.
ACCESSION   DBNGSB010000132 DBNGSB010000000
VERSION     DBNGSB010000132.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4169)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3774144"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="AC159B_03625"
     CDS             complement(<1..329)
                     /locus_tag="AC159B_03625"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015747.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0052851 - ferric-chelate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HIR3376873.1"
                     /translation="MSSLGPVAIVGGTGRLGMALAKRLSRAEIPVIIGSRDPAKAKSK
                     ATSLGPAARGFSNADAAAAGRVIILSIPYAAHRLTVASLAAGAGGKVVIDTAVPLVTG
                     EDPHVDLP"
     gene            complement(326..1606)
                     /locus_tag="AC159B_03630"
     CDS             complement(326..1606)
                     /locus_tag="AC159B_03630"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3376874.1"
                     /translation="MLTASHLPITPDSVTRTELHNGLVLLVREQRGSGMVALQGFVRA
                     GAMYDGERSGLARFTTAMLQRGTQTRSSHEIAEAFEGMGSILSLRIDLETIGIGLRAL
                     REDFRKAVEILSDVLSAPLFPSDEIERARGEILTGIRVGLQDTRQVAERTFRRLVFPP
                     GHPHAQVPDGDEVVVTALGRRELEAFHHDYVRPESTVLAIVGDVTAADAISLTSEFLS
                     GWPRRGIWSLPPIPPPRTATGAVRRDARMSGKVQSDLIIGVSGIARTDPAYYKAMAAN
                     LILGQIGLMGRLGESVREQQGMAYYAYSDLRAGLLPGPWWIRAGVNPANEERALSSIL
                     DEIKRFQDNGPDASELADAREYLIGSMAIRLETNGGIAQTLADIELYGLGLDHLERYP
                     GIIRKLGAEDLRQAATRLSTRDYYAAIAGPPAGA"
     gene            complement(1608..2933)
                     /locus_tag="AC159B_03635"
     CDS             complement(1608..2933)
                     /locus_tag="AC159B_03635"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3376875.1"
                     /translation="MRDLHIHTLANGLTVLLQSRHTAPVATFWVWYRVGSRNESPGLT
                     GISHWVEHMMFKGTPAHPGKALARYVDRLGGRWNAFTWKDYTAYHEVLPAEHLEVVVK
                     LEADRMRDTIFDPAEVDRERTVIISEREGSENFPSYLLYEEIDAAAFKTHPYRSPVIG
                     WKPDLRGITRDDLFSHYRSFYAPNNAVVVAVGAFDTENALELIRRSFEPIPPAAVPPM
                     RVTEQVQEGERRVTLERPGGATAYLHFAHHVPGAQDSDLAALLLLDGILSGFKGVVPF
                     DQPQGSRSSRLYRALVETSLAADVSSSLIPSIDPTLFRVTATVRAGVDPRSVEERAVG
                     ELQLLGREPVDFDELAKVKKQARAQIVYSRDGVYRTAMGLGAFAMVDKPEAFGTLLER
                     LERVTPDDILRVAATYLHSKNRTAGTYLPEAGAITAVPVAAAYSVREGD"
     gene            3019..3159
                     /locus_tag="AC159B_03640"
     CDS             3019..3159
                     /locus_tag="AC159B_03640"
                     /inference="COORDINATES: protein motif:HMM:NF048558.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7130 family rubredoxin-like protein"
                     /protein_id="HIR3376876.1"
                     /translation="MPFSAAKPTHYECTNCGQVLPRDRELPDHCPNCGQPKEAYVLIE
                     ED"
     gene            3202..>4169
                     /locus_tag="AC159B_03645"
     CDS             3202..>4169
                     /locus_tag="AC159B_03645"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002883.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HIR3376877.1"
                     /translation="MTRKILHVDMDAFFAAVEQLRRPELRGRPVVVGGDGNPHKRGVV
                     STASYEARAYGIHSAMPLRTAFKKCPHAVFLAVDFSAYGTYSRKIRSILGEYSELVEP
                     MSLDEAFLDVSHRPEEPVALAEEIRQRIKAETGLTASIGIGPNKLLSKIACGLRKPDA
                     VTEIKAETAIDQLHALPATVLWGVGPKTAARLEAALGVRTVGDLQGLALETLQGHLGP
                     RHGEYLYHVCRGEDDSPIVTDWEPKSLSRETTYQVDARKRDTVITTIDELAADVIKEL
                     RHEGYRGKTVTVKIRYRDFKTHTRALTLPLPTDDRATIRQTAVALL"
BASE COUNT          821 a         1329 c         1235 g          784 t
ORIGIN      
        1 ggcaagtcca catgtgggtc ttcgccggtc accagcggga cggcggtgtc aatgacgacc
       61 ttcccgccgg cacccgcggc cagagatgcc acggtgagcc ggtgcgccgc atagggaatc
      121 gacagaatga tcacacgacc cgccgcggcc gcgtcggcat tcgagaagcc tcgcgccgcc
      181 ggcccaagcg acgtggcctt ggatttcgct ttcgctgggt ctcgtgatcc tatgatgacc
      241 ggaatttccg cacgtgagag tctcttcgcg agcgccatac ccagccggcc ggtgcccccg
      301 acgatcgcaa cggggccgag actgctcacg cgcccgccgg tgggcccgcg atcgccgcgt
      361 agtagtctcg agtggagagc cgggtggccg cctgcctcaa atcctccgcg ccgagtttcc
      421 ggatgattcc cggatagcgc tcgaggtgat cgaggcccag cccgtataac tcgatgtcgg
      481 ccagtgtctg cgcaatgccg ccattcgtct ccagccgaat ggccatcgat ccgatgagat
      541 attcgcgagc atcggcgagc tcacttgcgt cgggtccgtt atcttgaaac cgtttgatct
      601 catcgagaat actcgacagc gcccgttctt cgttggccgg attgaccccg gcgcggatcc
      661 accagggtcc aggaaggagt cccgcgcgga gatcgctgta cgcataatac gccatcccct
      721 gctgttctct gacactttca ccgagccggc ccatcaagcc gatctgaccg aggatcaggt
      781 tcgccgccat tgccttatag tacgcggggt ccgttcgagc aatgccggac actccgatga
      841 tcagatctga ctgcactttc ccactcattc gcgcgtcccg tcgcacggcg ccggttgccg
      901 ttcgcggcgg agggattgga gggagcgacc atatgccgcg acggggccag ccggagagga
      961 attcgctggt aagagatatg gcgtcggcgg ctgtgacgtc acctacgata gccagcacgg
     1021 tggactccgg tcgcacatag tcatggtgga atgcttccag ttcgcgccgc cccagcgctg
     1081 tcacgacgac ctcatcccca tcgggtacct gcgcgtgggg atgtccaggc ggaaatacga
     1141 ggcggcggaa ggtacgttcg gcgacctgcc gggtatcctg gagaccgacg cggattccgg
     1201 tgagaatctc tcctcgagcc ctctcgattt cgtccgacgg aaacagaggc gcgctcaaca
     1261 catcgctgag gatctcaacc gctttcctga agtcctcccg cagcgcgcgg aggccgatac
     1321 caatggtctc caggtcgatc ctgaggctga ggatcgagcc catcccctcg aacgcctcgg
     1381 cgatttcatg cgaggacctc gtctgtgttc cacgctggag catggcggtt gtaaaccggg
     1441 cgagaccgct gcgctcgccg tcgtacatgg cgccggccct gacaaatcct tgaagcgcca
     1501 ccatgccgct gccgcgttgc tcacgcacga gcagcacgag cccgttgtga agttcggttc
     1561 ttgtgactga atccggagtg atcggtagat gcgacgcagt cagcaattca atccccttct
     1621 ctgaccgagt atgccgccgc aacgggaaca gccgttatcg cccccgcttc cggaagatac
     1681 gtgcctgcgg ttcggttttt tgaatgaagg tacgtcgcag caacccgcag gatgtcgtca
     1741 ggcgtcacgc gctccaaccg ttcaagaagt gttccgaacg cctccggctt gtccaccatc
     1801 gcgaacgcgc ccaaccccat cgccgtccgg tagacaccgt cacgcgaata gacgatctgc
     1861 gccctcgcct gctttttcac tttcgccagc tcgtcgaagt ccaccggctc gcgccccaac
     1921 aactgaagct cccccaccgc gcgctcctcg accgacctgg gatccacacc ggcgcgcacg
     1981 gtggcggtca cccggaacag cgtagggtcg atgctcggga tcaacgagga gctcacgtcg
     2041 gccgcgagag aggtctcaac gagcgcgcga tagagtcgcg aactacgact cccctggggt
     2101 tgatcgaagg gcacgacccc tttgaatccc gagagaatcc catcaagcag cagcagcgcc
     2161 gcgagatccg agtcttgggc accaggaaca tggtgcgcga aatgtaggta tgccgttgct
     2221 ccacctggtc tctccaaggt gacgcgccgt tccccttcct ggacttgttc tgttacacgc
     2281 attggaggca cagccgccgg cggaatcggc tcaaacgatc gccggatcag ctccagggca
     2341 ttctcggtgt caaacgcacc cacggcgacg acgacggcgt tgtttggcgc atagaaggat
     2401 cgataatgac tgaagagatc gtcacgggtg atgcctcgaa gatccggttt ccagccgatc
     2461 acaggagacc ggtacggatg cgtcttgaac gccgcggcgt cgatttcttc atacaacagg
     2521 taagacggga agttctcgct gccctcgcgt tcggagatga tcacggtccg ttcccggtct
     2581 acctctgctg gatcaaagat cgtgtcgcgc atgcggtcgg cttcaagttt cacgacgact
     2641 tcgagatgct cagcgggcag cacttcgtgg taggccgtat agtctttcca ggtgaaggcg
     2701 ttccaacgcc ctccaagacg atcgacatag cgcgccaatg ccttgcccgg gtgggccggg
     2761 gttcctttga acatcatgtg ctccacccag tgcgaaatgc cggtcagccc cggcgattca
     2821 ttccggcttc cgactctgta ccacacccag aaggtggcga ccggcgctgt gtgcctggac
     2881 tgaagaagta cggttagccc attggccagt gtgtgaatgt gaagatcacg cattattcga
     2941 gcgattctcc tcccccgctt ctatcccctt gaggcatata ctgaggacat ctgggagaac
     3001 gtcgaggagt gaccttctat gccattttcg gcagccaagc ccacgcacta tgagtgcacg
     3061 aactgcggtc aggtcctccc ccgcgaccgg gaactgcctg accactgccc gaattgcgga
     3121 caacccaagg aagcctatgt actgatcgaa gaagattaga taggtgacct ccgctctaac
     3181 tggacgcctg gtggagcgag cgtgacaagg aagatccttc acgtcgatat ggatgcgttc
     3241 ttcgccgctg tggagcagct ccggcggccg gagcttcgag gaaggcccgt ggtcgtcgga
     3301 ggcgacggca acccgcacaa gcgtggcgtc gtctccaccg cctcctatga agcgcgtgca
     3361 tacggcattc attctgccat gccgctccgc accgcattca agaagtgccc acacgcggtc
     3421 ttcctggccg tcgacttctc cgcctacgga acgtactccc ggaaaatccg gtccatcctt
     3481 ggcgagtact ccgaattggt cgagcccatg tctctcgatg aagcgttcct cgacgtgtcg
     3541 caccggccgg aagagccagt ggcgctggcc gaggagatca gacagaggat caaagcggaa
     3601 acggggctca cggcctcaat cggcatcggc ccgaacaaat tgctctcgaa gatcgcctgc
     3661 ggcctgcgca agccggacgc ggtaaccgaa atcaaagctg agacggcgat cgaccagctt
     3721 cacgccctcc ccgccaccgt gttatgggga gtcggaccga agacggccgc ccggctcgaa
     3781 gcggcgcttg gagttcgcac agtgggtgac cttcaaggac tcgccctgga gactttgcag
     3841 ggacacctcg ggccgagaca cggtgagtat ctctatcatg tctgccgcgg cgaggacgat
     3901 agtcccatcg tcacggattg ggaaccgaag tcactgagcc gggagacaac gtaccaggtc
     3961 gatgcccgaa agcgcgacac cgtgatcacg accatcgatg aacttgcggc agacgttata
     4021 aaggaactcc gtcacgaggg gtatcgcggc aagactgtga cggtgaagat tcgttatcgt
     4081 gacttcaaga cccacacccg cgcgctgacc ctgccgttgc ccacagatga ccgcgccacc
     4141 atacgacaga cggccgttgc gctgctcga
//
LOCUS       DBNGSB010000133         2981 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_860525, whole genome shotgun sequence.
ACCESSION   DBNGSB010000133 DBNGSB010000000
VERSION     DBNGSB010000133.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_860525"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1390)
                     /locus_tag="AC159B_03650"
     CDS             complement(143..1390)
                     /locus_tag="AC159B_03650"
                     /inference="COORDINATES: protein motif:HMM:NF016032.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - modified amino acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HIR3376878.1"
                     /translation="MAERYAARFRETVGPIRRVGRDAEFPLVYADGRAGEVARMWEPL
                     MSGDEFVPQYDDPKTLSLIEALTGEVGRIEVEVGRATLELVLGPYDDLCQLEEGSRRL
                     VRQVAQIADQLGMHVLGFGIQPRSRASSALMTPKQRYVHLARAAGPAWWRFTTTAADQ
                     IHVDITRTEITDAVNVLNLLSAPLIALTANSSVYAGRAGRYLSGREGLVGALGEHRAG
                     MTPGPFNSVEEFVRYICRYRCYVLRRDGDFRLYNRPFEEYLQRHGPDIEAYLWHEHYT
                     WNSARPRAHNSTIEIRPACQQPHEEPLVVAALALGWVEALPEVRAFINDALDNPWTSM
                     PRYRRDAIVHGLRSREPVERFVERALRIAEAGLRRRGRGEEQFLGPLWTRLEQRKTPG
                     DRARDLVRSRGIEALVDAAGYHL"
     gene            complement(1420..2034)
                     /locus_tag="AC159B_03655"
     CDS             complement(1420..2034)
                     /locus_tag="AC159B_03655"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR3376879.1"
                     /translation="MRHGLTELNRDKRVSGQTLDVPLLDEGIRQAQEARERFAGTPLD
                     VVISSPLMRAIQTAEIVTGVPSPGIVIDPLCNERSFGLMEGLRRAEVEARFPEVRYLQ
                     IGHVGYSLNPPGGELFPALHDRARRFLQGALQRHRGRRILISSHQTFLQQLHGVLRGL
                     EPFEALAHDILNLELNLFHLGRQDELLSHRRELLVPSSGKFPSF"
     gene            complement(2085..>2981)
                     /locus_tag="AC159B_03660"
     CDS             complement(2085..>2981)
                     /locus_tag="AC159B_03660"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl transferase family protein"
                     /protein_id="HIR3376880.1"
                     /translation="LDKPPGSVTYTQMLNTRGGVECDLTITRLESTQFFIVTGSAFGV
                     HDLAWIRTHLPEDGSVQARDVTTELTCIGLWGPRARDILQEISGGELSNGAFPYMTYR
                     PLEIAGAQVRALRVTYVGELGWELYMPVPDGLAVWDAIWESGQPLGLRPVGYRAVDSL
                     RLEKGYRYWSADVTPDYTPYEAGQGFCVKLDRKNFQGREALVRQREAGLTRKLCCLVL
                     NDRTVHPLGNEPVLVDRRVVSRTTSGGIGYTVGESIAYAYLPLALAEVSTELAVEIDG
                     RSVPARVEREPRFDPTNSRIKA"
BASE COUNT          544 a          957 c          939 g          541 t
ORIGIN      
        1 gcgaatgcgg tccaaggtag tccgatccta acattaacgg cggcgggatg gggaccttgg
       61 gcgaagggac ccgtaactaa gtcactcctt agccgatctc cgggtcccgg tccaaggtgc
      121 ccatcccgct gcagctcgtg cgctacaaat gatagccagc agcgtcgacc agtgcctcga
      181 ttccgcgaga ccggaccagg tcacgggcgc gatccccggg agtctttcgc tgctccaagc
      241 gggtccagag cggcccgagg aactgttctt cgccgcgccc ccgccggcgg agtccggcct
      301 cggcgatccg cagggcgcgt tccacgaaac gctcgaccgg ctctctgctc cgcaatccgt
      361 gcacgatcgc atcacgccgg taacgcggca tcgaggtcca gggattatcg agcgcgtcgt
      421 taatgaaggc ccggacctct ggcagggcct ctacccatcc aagggccagc gcggccacga
      481 ccagcggttc ttcgtgcggc tgctggcagg cgggacggat ttcaatcgtt gaattgtggg
      541 cccgcgggcg ggcagagttc catgtgtaat gctcgtgcca cagatatgct tcaatgtcgg
      601 gaccgtgcct ctgaaggtac tcttcaaacg gccggttgta gaggcgaaaa tctccatcgc
      661 gccgcaggac gtagcaccgg taccggcaga tgtaccggac aaactcctcg accgaattga
      721 acggcccggg tgtcatcccc gcgcggtgct cccccaacgc gcccaccagt ccttcgcgtc
      781 ctgacaggta ccggccggcc cgtcctgcat agacggatga gttggccgtg agcgcaatca
      841 gcggagccga cagcaggttc agcacgttga cggcgtcggt gatctctgtc cgtgtgatgt
      901 ccacatgaat ctggtcggcc gccgtcgttg tgaagcgcca ccaggcggga ccggctgcgc
      961 gtgccagatg cacgtaccgc tgcttgggcg tcatcaacgc ggatgacgcc cggctccgcg
     1021 gctgaatccc aaatccaagt acatgcatcc cgagctgatc ggcaatctgc gccacttgcc
     1081 gtacaaggcg ccgggagcct tcctccagtt gacagagatc gtcgtacggc ccgaggacaa
     1141 gctctagggt ggcgcgtccc acttcgactt caattctccc gacctcgccg gtgagcgctt
     1201 caattaatga gagtgtcttt gggtcatcgt attgagggac aaactcgtcg ccgctcatca
     1261 acggttccca catgcgtgct acttctcccg cccgcccgtc cgcgtacacg aggggaaact
     1321 ccgcgtcccg cccaacacga cggatcgggc cgacagtctc gcggaagcgc gccgcgtagc
     1381 gttccgccaa gactccgtag gtctgctgaa tgccccccat taaaaggatg ggaatttgcc
     1441 ggacgaaggg acgagcagtt cgcgacgatg gctgagaagt tcgtcttgcc gacccaggtg
     1501 gaacaggttt agttccaggt tcaagatgtc gtgcgccagc gcctcgaagg gttcgagtcc
     1561 tcgaagaacg ccgtggagct gctggaggaa ggtttggtgg gatgagatga ggatccgccg
     1621 gccgcgatgg cgctgcaatg ctccctgtag gaacctgcgc gcgcggtcgt gcagggcggg
     1681 gaacagctcg ccgccaggcg ggttcagcga ataacccacg tgtccaatct gcaagtaccg
     1741 cacctccgga aagcgcgcct ccacttccgc gcgcctcagc ccttccatca gtccgaagga
     1801 gcgctcgttg cacagtggat cgatcacgat gcccggcgaa ggaacgcccg tcacgatctc
     1861 cgccgtttgg atggcccgca tgagcgggga tgaaatcacg acgtcgagcg gcgtgccggc
     1921 aaaacgttcg cgtgcctcct gagcctggcg gattccttca tccaggagag gcacatccag
     1981 ggtctgaccg ctgacccgct tgtcccggtt gagctcggtc agcccgtgcc gcacagtaag
     2041 cagcagtgtt tcgtctggtg cgaggagcgg aatcccgatc ggaattaggc cttgatgcgg
     2101 ctgttggtcg ggtcgaatcg cggctcgcgt tccacccgcg cggggacaga tcggccgtcg
     2161 atctcgacag cgagctcagt ggagacctcc gccagcgcga gaggcaggta ggcgtacgcg
     2221 atgctctcgc cgacggtata cccgatccca ccgctggtgg tccgagaaac cacacgccga
     2281 tcaacaagca caggctcatt gcccagagga tggaccgtcc gatcgttgag gacgagacag
     2341 cagagcttgc gcgtcaggcc cgcttcccgc tgccgcacta acgcctccct tccctggaaa
     2401 ttttttcggt cgagtttgac gcaaaagccc tgtccggctt cgtagggcgt atagtcgggc
     2461 gtgacgtccg cgctccaata ccggtaccct ttctcgagtc tgagggagtc gaccgcgcga
     2521 tagcccaccg ggcgcaaccc gaggggctgt cccgattccc agatcgcatc ccagaccgcc
     2581 agtccgtctg ggacgggcat gtagagttcc catccgagtt ctcctacgta cgtgacgcgc
     2641 agcgcgcgca cctgggcgcc ggcaatttcg agggggcggt acgtcatgta ggggaacgcg
     2701 ccgttggaga gctcgccccc gctgatctcc tggagaatgt ccctcgcccg tgggccccat
     2761 agaccgatac aggtgagttc ggtcgtgacg tcccgcgcct gcactgaacc gtcctcgggg
     2821 aggtgagtcc gaatccaggc gaggtcgtgc acaccgaacg cgctcccggt cacgatgaag
     2881 aactgggtcg actccaggcg cgtgatcgtg aggtcgcatt cgacgccgcc gcgcgtgttg
     2941 agcatctgtg tgtacgtgac cgatccgggc ggcttgtcca g
//
LOCUS       DBNGSB010000134         4671 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_1320507, whole genome shotgun sequence.
ACCESSION   DBNGSB010000134 DBNGSB010000000
VERSION     DBNGSB010000134.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4671)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_1320507"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="AC159B_03665"
     CDS             <1..467
                     /locus_tag="AC159B_03665"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HIR3376881.1"
                     /translation="PTGDIGYLFVDDFNVEVQLEGPAAPAPEIVQPFDVVREAMRYLG
                     VRYVWGGESARGMDCSGFIYLVFQSRLPGLERMRSFDYFRMSTPIAPSALVPGDLVFF
                     STYKAGPSHLGIYIGEGKFIHASSGYGVVAITPLDDPYYAARFVGARRLVKP"
     gene            603..1910
                     /locus_tag="AC159B_03670"
     CDS             603..1910
                     /locus_tag="AC159B_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004067418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HIR3376882.1"
                     /translation="MILGAGGRDFHNFNVYYRGRAEFEVVAFTATQIPEIEGRRYPRE
                     LAGSRYPNGIPIVAESELASLIGKHGVHEVAFSYSDVSHDHVMHLASTALAAGATFVL
                     LGPDATMLEAKVPVVSVCAVRTGAGKSQSTRRVASILKHAGRKVAVIRHPMAYGDFVR
                     QRVQRFATFEDLERADVTIEEREEYEPHLRAGTLVYAGIDYAEIVAIAEREADVIVWD
                     GGNNDFPFVRSQLHIVIADPHRPGHELSYHPGETNLRMADVVVINKVDTARAEGVAEI
                     RTNIRAVNPGATIVEAASPLQIDDESLIRGRSVLVVEDGPTVTHGGMPYGAGTIAARK
                     FGAGEIVDPRIVAVGSIRDVYQRYPHLGPVLPAMGYGRKQIRELEETINACACDTVII
                     GTPVDLRRLLRISRPAVRVTYELHELTKPDLEDILREWLAKLT"
     gene            1913..2839
                     /gene="argF"
                     /locus_tag="AC159B_03675"
     CDS             1913..2839
                     /gene="argF"
                     /locus_tag="AC159B_03675"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013312250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIR3376883.1"
                     /translation="MALKARDFVSIDDLSVEELNEVLDFAVDLKRRMKGGDRPPVLAG
                     KVLALLFEKPSLRTRVTFEVGMLQLGGHAVYLSPSDVQMGSRESVPDVARNLERWVDA
                     ILTRTFAHRTVEELAEHAGVPVINGLSDLEHPCQVLADVLTIRERVGEVRNVRVAWVG
                     DGNNVCHSLLLGMAKLGAQLSVATPRAYGPDPRILARAQEIAGGTRASITAGTDPHEA
                     VRDAQVVYTDVWVSMGQESERAVKRQAFSGFQINAALLGRAAKDVIVLHCLPAHRGEE
                     ITAEVLDGPRSAVFDQAENRLHAQKALLGKLL"
     gene            2845..3657
                     /gene="cdaA"
                     /locus_tag="AC159B_03680"
     CDS             2845..3657
                     /gene="cdaA"
                     /locus_tag="AC159B_03680"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HIR3376884.1"
                     /translation="MPSLRILDILDILIVTILVYQLLMLIRGTRAVQLVTGLGVLVLA
                     YAVSRYLRLYTLQYLLGYLGLVIPIALLVLFQPELRRMLEQLGRGGVLVTGLTPLSLG
                     REEAIRLVNDVARAARVLSMRKIGALMVLERRTGLQDFIDTGIKIDALVTVQLLINLF
                     FPNSPMHDGAAIIRGNRVIAAGCLLPLSENMSIGRTLGTRHRAGVGIAEQADAVAIIV
                     SEETGTISLARDSEMVRGLSEEELKAALLSLIAAEPARAAMPWRWWRLTPRT"
     gene            3660..4304
                     /locus_tag="AC159B_03685"
     CDS             3660..4304
                     /locus_tag="AC159B_03685"
                     /inference="COORDINATES: protein motif:HMM:NF019561.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HIR3376885.1"
                     /translation="MRRVLSEQNFMLLLSLLTAIGLWMYVTLTRAPVEFGTPKSVAVI
                     PTIVGTPAIGYSVLGVRVVPQTVNLSGNPARLATVESAMTEPVNINGAIQDVVQEVSV
                     ATPLGLTAARRIQVSIQIAPAMAVTLVPGVRVEIRNVPPGAVAEVEPKTIQVQVQGPV
                     SVVSRLRPEDFLARVDGDMDEGRRRVKVIIDAPQQVELLSVMPAEVVVTVRKGG"
     gene            4341..>4671
                     /locus_tag="AC159B_03690"
     CDS             4341..>4671
                     /locus_tag="AC159B_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011461863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HIR3376886.1"
                     /translation="MGRLFGTDGVRGVANVDLTPELVFTLGRAAAHVLNPDGPGRFLI
                     GRDTRLSGGMLEAALVAAICSTGSHPVRGGVLPTPAVAYLTPMQRAAAGVVISASHNP
                     IGDNGIKF"
BASE COUNT          855 a         1367 c         1539 g          910 t
ORIGIN      
        1 tccccaccgg tgacattggc tatctcttcg tcgacgactt caatgttgaa gtgcagcttg
       61 agggtccagc tgcacctgcg ccggagatcg tgcagccctt cgatgtggtc cgcgaggcga
      121 tgcgctacct cggggtacgg tacgtgtggg gtggcgagtc ggcgcgcggc atggactgct
      181 caggcttcat ttacctcgtg ttccagagca ggctgccggg gcttgaacgg atgcgttcgt
      241 tcgattactt ccggatgagt acgcccattg ctccctccgc gctcgtgccg ggagatcttg
      301 tgtttttctc gacgtataaa gccggaccgt ctcatctcgg gatctatatt ggtgagggaa
      361 agttcatcca cgcgtcttcc ggctacgggg tggtcgcgat cacgccgctg gacgacccat
      421 actatgccgc acggtttgtg ggagcccgga ggctcgtgaa gccttagcgc cgaagatgct
      481 gattcgtggc taggcgcgag caacgaagca acgcccggca ggtcaccccc cgaagaagac
      541 gaccgcaagg caggcctcga ccatcgaaat ttgaccagcg gccctccgtc ctccgccggg
      601 tcgtgattct cggcgccggt ggacgagact ttcacaactt caacgtgtac tatcggggcc
      661 gggccgagtt tgaagtcgtc gcgttcaccg caacgcagat ccccgagatc gaaggtcggc
      721 gttatccgcg cgagttggcc gggtctcggt acccgaacgg catccccatc gtcgcggaaa
      781 gcgaactcgc gtcgctcatc gggaagcacg gggtccacga agttgcattt tcgtacagtg
      841 atgtgtccca tgaccacgtc atgcaccttg cgagcacggc gcttgccgcc ggggcgacct
      901 tcgtgctgct cggtccggac gcgaccatgc tcgaggcgaa ggttccggtg gtgagcgttt
      961 gtgcggtcag gaccggcgcc ggcaagagtc aatcgacacg ccgggtcgcg tccatcttga
     1021 agcatgcggg caggaaggtg gccgtgatcc gccatccgat ggcctacggc gacttcgtgc
     1081 ggcagcgcgt ccagcgattc gcaacgttcg aggatctcga gcgcgccgat gtgaccattg
     1141 aagagcggga ggagtacgag cctcatctcc gagccgggac cctcgtctat gcaggcatcg
     1201 actatgcgga gattgtcgca atagccgagc gtgaggcgga cgtgatcgta tgggacggtg
     1261 gcaacaatga cttcccattt gtccgaagcc agctgcacat cgtgatcgcc gatccccacc
     1321 ggcccggaca tgaactcagt tatcatcccg gcgagacgaa ccttcgcatg gccgacgtgg
     1381 tcgtcatcaa caaagtggat acggcgcggg cggaaggtgt ggctgaaata aggaccaaca
     1441 ttcgggccgt caacccgggc gcgacgatcg tcgaagcggc ttcgccgctc cagatcgatg
     1501 atgaatctct gattcgcggc cgatctgtcc tggtggtcga ggatggtccc acggtgaccc
     1561 acggcgggat gccgtatggc gccgggacga tcgcggcccg gaagttcgga gccggcgaga
     1621 tagtcgatcc ccggatcgtg gcggtcgggt cgatccggga cgtgtaccag cgctatccac
     1681 acctgggccc cgtgcttccg gccatgggct acgggaggaa gcaaattcgc gagctcgagg
     1741 agaccatcaa cgcgtgcgca tgtgatacgg tcatcattgg gacgcctgtc gacctccgcc
     1801 gcctgctacg catttccagg ccggccgtcc gcgtgaccta tgagctgcat gaactgacga
     1861 agcccgacct ggaagatatt ctgagggagt ggcttgcgaa actgacgtaa caatggcgct
     1921 caaggcacga gatttcgttt caatcgacga cctctctgtt gaagaactca acgaggtcct
     1981 cgacttcgcg gtcgacctga agcgtaggat gaagggcggc gaccggccgc cggtgttggc
     2041 tgggaaagtc ctcgcgctgc tctttgagaa accgtcgctc cgcacacggg tcacgttcga
     2101 agtgggcatg ctgcaactcg gcgggcacgc ggtctacctg tcacccagcg atgtacagat
     2161 gggctcgcgt gaaagtgtgc ccgacgtggc ccggaatctt gagcgctggg tcgacgcgat
     2221 cttgacccgg acgttcgccc atcgcacagt agaagaactt gcagaacacg ccggcgtccc
     2281 ggtcatcaat ggcctctctg atctggagca cccgtgccag gtgcttgcgg atgtgcttac
     2341 catccgggaa cgagtcggag aggtccgcaa cgtgcgggtg gcatgggtag gagatggcaa
     2401 caacgtctgt cactcgctgt tgctggggat ggcgaaactc ggagcgcaac tgtcggtcgc
     2461 gacgccgcgg gcgtacggtc cggacccgcg gatccttgcg cgggcgcagg agatcgccgg
     2521 agggacgcgt gcgtcgatca cggcgggtac agatcctcac gaggccgttc gagacgccca
     2581 ggttgtctac accgacgtgt gggtgagtat gggacaggaa tcggagcgcg ccgtgaaacg
     2641 ccaagctttt tcgggatttc agatcaacgc agcgttgctg gggcgtgccg cgaaagacgt
     2701 catcgtgctg cattgcctcc cggcgcaccg aggcgaagag atcacggctg aggtgctcga
     2761 tggaccgcga tcggcggtct tcgatcaggc tgaaaacagg ttgcatgcgc agaaggcgct
     2821 gctgggcaaa ctactgtaac tgagatgcca tcgctgcgaa tcctcgacat cctggatatc
     2881 ctgattgtca cgatcctcgt gtatcagctg ctgatgctga tccggggcac aagagcggtg
     2941 cagctggtga caggtcttgg ggtcctggtg ctggcgtatg ccgtcagccg ctacttgagg
     3001 ctgtacacct tacagtatct cctcggatac ctcgggctcg tgatcccgat cgcgttgctt
     3061 gtgctgttcc agcccgagct gcggcggatg ctggaacaac tgggccgggg tggggtgctg
     3121 gtgacagggt tgacgccgct gtcactcgga cgcgaagaag cgatccgact ggtgaatgac
     3181 gttgcccgcg ccgcgcgcgt cctgtcgatg cggaagatcg gagcgttgat ggtgcttgaa
     3241 cggcggacgg gactccagga tttcatcgac accggtatca agatcgatgc gctcgtgacc
     3301 gtgcagctgt tgattaacct gttcttcccg aactccccga tgcatgacgg tgccgcgatt
     3361 atccgaggaa accgcgtcat cgccgcgggc tgcctgttgc ccctcagtga gaacatgtcg
     3421 atcggccgga cactcggtac ccgacaccgc gcgggcgtgg gcatcgccga acaggccgat
     3481 gcggtggcga tcattgtgtc cgaagaaacg ggtacgattt cactggcgcg ggatagtgaa
     3541 atggtcagag gtttgtccga ggaggaactc aaggccgcac tactgagcct gatcgccgcg
     3601 gagccggcgc gagctgcgat gccgtggcgg tggtggcggc tcacgcctcg gacctaacga
     3661 tgcgccgcgt cctgagcgaa cagaacttca tgctccttct ctccttgctg acggcgatag
     3721 gtttgtggat gtatgtgaca ctcacacgcg cgcccgttga attcgggacg ccgaagtccg
     3781 tggccgtgat cccgacgatc gttggtacgc cggcaattgg gtacagcgtg ctgggtgtgc
     3841 gtgtggttcc tcagaccgtg aacctttcgg gaaatccggc gcgtctcgcc accgtggaat
     3901 ccgccatgac cgagccggtg aatattaacg gcgcgattca agacgtcgtc caggaagtgt
     3961 cagtggccac gcccctgggg ttgaccgcag cccgacggat ccaggtatcc atccagattg
     4021 cgcccgcgat ggccgtgacg ctcgtgccgg gcgtacgcgt ggaaatccgc aatgtgcctc
     4081 ccggcgcagt cgccgaggtt gagccaaaga caattcaggt ccaggtgcag gggccggttt
     4141 ccgtagtcag ccggctgaga cccgaggact ttctggctcg agtggacggc gacatggacg
     4201 aagggcggcg aagggtgaaa gtgatcatcg acgcaccgca gcaagtcgaa ttgctctccg
     4261 tcatgccggc cgaggttgtg gtgacggtcc gaaaaggcgg atgactcctc tcgtcattgc
     4321 gagcgaagcg aaccaacctc gtgggtagac tttttggaac cgacggtgtg cgtggggttg
     4381 ccaatgtcga ccttacgccg gagctggtct ttaccttggg acgtgcagcg gcgcacgtgc
     4441 tcaatcccga tgggcccggc cggtttctga ttggacgcga cacacggttg tccggcggga
     4501 tgctggaggc cgcgctcgtg gccgcaatct gctcgaccgg gagtcacccc gtccgtggcg
     4561 gtgtcctgcc cacccccgcc gtagcctatc tcacccccat gcagcgtgcg gcggccggcg
     4621 tggtgatctc ggcgtcgcac aatccgatcg gagacaacgg catcaaattt t
//
LOCUS       DBNGSB010000135        11247 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2854909, whole genome shotgun sequence.
ACCESSION   DBNGSB010000135 DBNGSB010000000
VERSION     DBNGSB010000135.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 11247)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11247)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11247
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2854909"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="AC159B_03695"
     CDS             complement(<1..558)
                     /locus_tag="AC159B_03695"
                     /inference="COORDINATES: protein motif:HMM:NF020892.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR3376887.1"
                     /translation="MSDNRYYITTAIDYVNAGPHIGHAYEKIIADVLARFHRLRGDDV
                     YFLTGTDEHGQKNATTAAWAGKPVREFVDDNAALFKALAPILHLSIDDFIRTTEPRHA
                     KSVKALWERVAAAGDFYKKNYKALYCVGHEAFVGKSDLVDGKCPVHDTEPIIIEEENY
                     FFRLSKYRQHLRRLFEGRPDFVVPAS"
     misc_binding    complement(669..903)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(933..1244)
                     /locus_tag="AC159B_03700"
     CDS             complement(933..1244)
                     /locus_tag="AC159B_03700"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HIR3376888.1"
                     /translation="MMIRSMIRLSVVSGREREFESMFSRLRVLEKAKSAAGLITGQVL
                     KPVQGGSYAVTATWNSARDYQRWIDSPVRAELIKALDGVAVPDGAPELFEIVETYAPV
                     H"
     gene            complement(1251..2372)
                     /locus_tag="AC159B_03705"
     CDS             complement(1251..2372)
                     /locus_tag="AC159B_03705"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012904.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIR3376889.1"
                     /translation="MHQAGSESEALLVQRILQDAGIPVLVRSRQVPGYAEVIRGAIGV
                     WGDILVPKAYEPDALQYVQQYLRAMKEPTPVSRISGIIPPLVTLFDDRGRIDPGANEK
                     HVEFLIGRGVHGLFALGSTGEAMHLSADERREFAGLVVGQVKGRVPVLVGCLSTSTDE
                     AVLLARHAQEIGADGVVVIPPYYWTPNDTAIEMHIGAVARAVKLPVVIYNFPAVVGRI
                     VPVALVARLSQAFPTVLGIKETIDNVGHIHEVIARVKAGKPEFSVLCGYEFHLLNTLL
                     SGGDGAIPAIANFAPQLPVSLYEAYRKGRIEDAAAQMRSRLELATLYQLDAPFFVVVK
                     EAMTMLGLIASPTVRAPAPPLTDDGKTRLRGLLTSAELL"
     gene            complement(2411..3247)
                     /gene="rsmI"
                     /locus_tag="AC159B_03710"
     CDS             complement(2411..3247)
                     /gene="rsmI"
                     /locus_tag="AC159B_03710"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIR3376890.1"
                     /translation="MAGTLYVVSTPIGNLEDLTFRALRVLKEVEIIACEDTRHTRTLL
                     AHYGIDKRLLSYHEHNERQRTAELLQRLEENASVALLTDAGTPALSDPGYAIVHAAAA
                     RGIPVIAVPGASAVTAALAVAGLPTDRFAFLGFLPRKSGERKRALEALASIPWTLVFF
                     EAPHRLRDMLKDIADVLGDREVAVGRELTKRFEEVIRGSLGEVQEHFRDTEPRGEFTV
                     VVAGAGENVETAAVSAETDLKELLEGGMPPKNAVQIVARRHGVSRRAVYQMMLGMTGK
                     RQ"
     gene            complement(3254..3742)
                     /locus_tag="AC159B_03715"
     CDS             complement(3254..3742)
                     /locus_tag="AC159B_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015125334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HIR3376891.1"
                     /translation="MTTRVIGVIGESEFSDPSLQSVAEEVGRLIARAGYLLICGGLTG
                     VMEAACRGARSAGGRTIGILPGTDAREANAFVDISIPTGLGQGRNIIIALASDALIAI
                     GGGFGTLAELGHALRLGKPVVGLRTWDASRAGRRAPIAVVDTPQAALEEVARALAGLH
                     AR"
     gene            complement(3739..6087)
                     /locus_tag="AC159B_03720"
     CDS             complement(3739..6087)
                     /locus_tag="AC159B_03720"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3376892.1"
                     /translation="MDVRAFLDELKHRPGYEGQLVHERFIPPRTGRYAELRPPLLPAV
                     RESLAAQGITRLYRHQVEAIGAVRRGQHTVVVTTTASGKTLCYNLPVLETLLEDPSSR
                     ALYIFPTKALAQDQADALAEFGFPHLVLGTYDGDTPQDQRRRLRERAQILLTNPDMLH
                     IGILPQHYKWATVFRNLRYVVIDDVHVYRGVFGSNVANIIRRLRRICRMHGANPVFIC
                     TSATIANPKEFAGALLGLPVTVVDVDGSPSGPKWFALWNPPIIDEARARRRSTYSEAT
                     GLFTELIRRGVRTITFTQARKITELVYRYARLDLETHHPDLAARISPYRAGYLPEERR
                     AIERRLFHGDLIGVVSTSALELGIDVGSLDAAVLVGFPGTIASTWQRAGRAGRGTDEA
                     LVVLAALEDALDQYLMRNPDYLFERPVEHAVIDPENPYILAGHLKCAAAEVPLVEQDS
                     ELFGTSLPDVVKILQENGDLVPRLGRWYWAKRSYPANEVEVRSASGNSFRIVDGMSGR
                     LVGTVDSSRAFEQVHPGAIYLHQGESYLVQRLDLEQRIASVGATDADYYTQPRTTTDL
                     EILERIKQRPWGSTTAYFGQVEVTTQVIAYARKRLFSDEVLSEEPLDLPEERLQTTAL
                     WFPIPPALDDDVRRQGLDLAGGIHAAEHAAIGILPLFAMCDRWDLGGVSYPMYPELGS
                     AAIFIYEGHPGGVGITEKGFGLLDELMSATLAAIEACPCEAGCPSCIQSPKCGNMNEP
                     LDKAAAILLLRGLLGQDGRRRSPGAKKQARSRKHSPTHNARS"
     gene            6183..6269
                     /locus_tag="AC159B_03725"
     tRNA            6183..6269
                     /locus_tag="AC159B_03725"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6217..6219,aa:Leu,seq:cag)
     gene            complement(6328..7950)
                     /locus_tag="AC159B_03730"
     CDS             complement(6328..7950)
                     /locus_tag="AC159B_03730"
                     /inference="COORDINATES: protein motif:HMM:NF038114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PxKF domain-containing protein"
                     /protein_id="HIR3376893.1"
                     /translation="MLRHGKLGVLVALLLSISTAVVAAAPFAYITNSGSANILVIDTA
                     TETGVTPISLPAGTVPYGVAVSPDGSRVYVSHFAFQGKLFVVDTASNTVLASPAVGMF
                     PFGVAVHPSGNRVFVTTMQNGGRLVAIDATTFSVTPVFVGNPRGIAVHPDGSKIYVVN
                     YFNDSVSIMDAATLSSIGSIDVGVSPIGIAVSADGSRIYVTNEDSGTVSVINSATNAV
                     VATVTVGNNPVGVTVTPDGARVYVANSLSGTVSVIDAGTNMRIGDVHVGNHPYGISND
                     GAQVYVALVHDSKVVVINRATNAILKEFPANVPVAFGQFIRPASRIDSSMALHLEPAE
                     VIVGSSSTVVQRATLTRRDSGAGIDGATITFSVDGATLGSAVTASGGVATWNFNPSGL
                     EPGPHAVRAAFAESVIGGLTYGAANASASLRVQYHWLGWMPPVVGEGALNQAKAGSNV
                     PVKWQIKDASGNLINALSAVVGIQTALIPCAGDGASEFMAASSPGNAGLRYDGRQFHF
                     NWKTEKAWMGQCRAFMVELADGTTHMAKFQFR"
     gene            complement(8087..9448)
                     /locus_tag="AC159B_03735"
     CDS             complement(8087..9448)
                     /locus_tag="AC159B_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019120350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR3376894.1"
                     /translation="MEDIFDYIDQNKQPFIERLQWLCRQPSIAAQNVGIQETAGMVAK
                     LMEEIGIRPQLYATDGAPVVYGAAGSGTRTLLIYNHYDVQPPEPLELWESPPFAADVR
                     DGKLYARGSADNKGNLVARLCAVEAWLRTRGSLPLNVKFVVEGEEEVSSSHLHQFVRQ
                     HKDFIKSDGCIWEAGGKDIQENPGLYMGAKGILYVELEAKGANRDLHSSQAVVVPNPA
                     WKLIWALSTLKDRHEHILIDGFYDDVVEPTPVEMEYLRRIAANRDDDVRRRDLGIDQF
                     LLGVTGLQLVKRNLYQPTCTICGLVSGYTGQGSKTVLPSLARAKIDFRLVPEQRPDDI
                     LEKLKRHLAQQGFADIEVQVLGAEHPARTPVDSTLARVVEETAKEIYGREPVVSPTMA
                     ATGPMWELTAQFGIPTVGTGSGYAHSNGHAPNENIRLEDWFQHMRHVALIFDRFAKAP
                     RET"
     gene            complement(9459..9689)
                     /locus_tag="AC159B_03740"
                     /pseudo
     CDS             complement(9459..9689)
                     /locus_tag="AC159B_03740"
                     /inference="COORDINATES: protein motif:HMM:NF024590.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
     gene            complement(9702..10154)
                     /locus_tag="AC159B_03745"
     CDS             complement(9702..10154)
                     /locus_tag="AC159B_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376895.1"
                     /translation="MPFLNDQDAALVRKRFDNDLDHPVTLEFFTPSTGGLVLPGQDSE
                     MAEYTRQILAEVAALSPKITLNVHSLAAEPESAGAFGISRTPATVVIGAQDFGIRYFG
                     IPGGYEFATLLDLIIAVSQGKPPIADETREILSRLKNDAHIQVFVTPT"
     gene            10182..10556
                     /locus_tag="AC159B_03750"
     CDS             10182..10556
                     /locus_tag="AC159B_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376896.1"
                     /translation="MAAEFAIDFKVLEHLRGYPDELKRYSNLIKQVHPRGMSAVEFLL
                     KRPSAADDFVAQVCRLVRDGEEVMSVVEAAETASLEPRAFLDGVASDPEFPQPLFRKD
                     HKAVWRRADVEAFLARHTDRNG"
     gene            10549..10743
                     /locus_tag="AC159B_03755"
     CDS             10549..10743
                     /locus_tag="AC159B_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376897.1"
                     /translation="MGKLRLRVVCKECGREFDTPLVLDRKSFAKGTLAANYHTCPHCG
                     ERLTYRKADYLVSEEIREPR"
     gene            complement(10745..>11247)
                     /locus_tag="AC159B_03760"
     CDS             complement(10745..>11247)
                     /locus_tag="AC159B_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0044183 - protein folding chaperone
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HIR3376898.1"
                     /translation="SRTSSTARWAAVVLLAALVALSVPATAGRGPRDPRAERIVEALL
                     VWRLVDELNLSEPQIARIFPRIKALKELRIELGRRKITVHRELRDLMQQQPRNEEEIQ
                     AKITELDQLRGQIEFRRQGILREINAALTLEQRAQFALITETFEVETIRMLEELRLIV
                     EQRRRR"
BASE COUNT         2079 a         3544 c         3449 g         2175 t
ORIGIN      
        1 gctggccggc acgacgaagt cgggacgccc ctcgaacagc cgccgcagat gctgccgata
       61 tttcgagagg cggaagaagt aattctcttc ctcaatgatg atcggctccg tatcgtgcac
      121 gggacacttg ccgtccacca agtcactctt gccgacgaag gcctcgtggc cgacgcaata
      181 gagcgctttg tagttttttt tgtagaagtc gccagccgcg gccacgcgtt cccagagggc
      241 tttgacgctc ttcgcatgcc tcggctcggt cgtccggatg aaatcatcga tggagagatg
      301 cagaataggt gccagggctt tgaagagggc agcgttatcg tcgacgaact cccggaccgg
      361 tttacctgcc caggccgccg tggtggcgtt cttctgaccg tgttcgtcgg tccccgtgag
      421 gaagtagacg tcgtcgcccc gcaggcggtg aaatctcgcc agcacgtcgg cgatgatctt
      481 ctcatacgcg tgcccgatgt gcgggccggc gttcacgtag tcgatcgcgg tggtgatgta
      541 atacctgttg tcgctcacga cgctcacccc tcaccaggat ttcggatttc ggattgtcga
      601 ttgcgacttg catgcggctg agatccgaaa tcgtcaatcc gcaatccgaa atcaaaaagc
      661 ctccgtccct atgggacgag aggcatctct cgtggtacca cccagttttg acctcgttac
      721 agatacgggg agcggcgctc ccgatatcct ggcctgctat cggtggccta ccggcaaacc
      781 ctacttgtgt tcaggttcgc agctccggag ccatgttccc cgccgacctc ccgccggctc
      841 tcaccatacc cggctcgcta atgggattgg gcagcggcta ctccgctccg tcaacgcttt
      901 ggcgaccatt ctagggtgcg cccggcaacc tgtcaatgca ccggggcata cgtctccacg
      961 atttcgaaca gctccggtgc accatccgga actgccacac cgtccagcgc tttgatgagc
     1021 tccgcgcgaa ccgggctgtc gatccatcgc tgatagtctc tggcgctgtt ccaagtggct
     1081 gtgacggcat aagagccgcc ctgaaccggc ttcaagacct gaccggtgat cagacccgcg
     1141 gcggattttg ccttctccag cacacggagc cttgagaaca tcgactcaaa ctcccgctca
     1201 cgaccggaca cgaccgacag gcggatcatg cttcggatca tcatgcacct ctagagcagc
     1261 tctgcggagg tgagaagacc tcgcaggcgg gtctttccgt cgtcggtcaa aggaggcgcc
     1321 ggagcgcgaa cggtcgggct ggcaatgagc ccgagcatcg tcatcgcctc cttgacgacc
     1381 acaaagaacg gagcatccag ctgatagagc gttgcgagct cgagccggct ccgcatctgg
     1441 gctgcggcat cctcgatacg cccttttcta tacgcctcat acagcgatac aggcaattgt
     1501 ggcgcgaagt tcgcgatggc cgggatcgcc ccgtcaccgc cggagagcag cgtgttcagg
     1561 aggtggaact catatccaca cagcacgctg aactccggct taccggcctt caccctcgca
     1621 atcacctcgt ggatatgacc gacgttgtcg atcgtctcct tgatcccgag cacagtgggg
     1681 aacgcttgag agagccgcgc cacgagtgcc acggggacga tccgtccgac cacagccggg
     1741 aagttgtaga tcacgacggg caacttcacc gctcgcgcca cggctccgat atgcatctcg
     1801 atcgcggtgt cgttgggcgt ccaatagtat ggagggatga ccacgacgcc gtcggcgccg
     1861 atctcttgcg catggcgcgc gagcagcacg gcttcatcgg tcgacgtgga caggcatccc
     1921 acgagcacgg gcacgcggcc cttgacctgc ccgacgacaa gaccggcgaa ttcccgccgt
     1981 tcgtccgcgg acaagtgcat cgcttcaccg gtgctgccta acgcaaagag gccgtgcacg
     2041 ccgcggccga tcaggaactc gacatgcttt tcgttggctc cgggatcaat gcggccgcga
     2101 tcgtcgaaga gggtgacgag cggcgggatg atacctgaaa tccgggacac cggggtgggc
     2161 tccttcatag cgcgcagata ctgttgcacg tactgcagcg catcgggttc atacgctttg
     2221 ggaaccagga tatcccccca gacgccgatc gcgccgcgga tcacttccgc atagccgggg
     2281 acctggcggc tgcgcaccag gaccgggatg cccgcatcct ggaggatgcg ttgcacgagc
     2341 agcgcttcgg attcgctgcc cgcctgatgc acccgggcgt aatcggtggg aggaggcaac
     2401 cagtgttgca tcattgccgc ttgcccgtca tccccaacat catctggtaa actgcgcggc
     2461 ggctgacgcc gtgccggcgg gcgacgatct gtaccgcgtt cttcggtggc atgccgccct
     2521 ccagtaactc tttcaagtcc gtctccgcgc tcaccgccgc cgtctcgacg ttctcccccg
     2581 cgccagccac gacgacggta aactccccac gcggctcggt gtcgcggaaa tgctcttgga
     2641 cctcacccag cgatccacgg atcacctctt caaatcgctt ggtcaactcc cgccccaccg
     2701 ccacctcacg atcccccagt acgtctgcta tgtctttcag catatcgcga aggcgatgcg
     2761 gcgcctcgaa gaacacgagc gtccacggga tggaggccag cgcctcgagc gcccgcttcc
     2821 tctcgccgga tttgcggggc agaaatccaa ggaaggcaaa ccgatcggtg ggcagccccg
     2881 cgactgccag cgccgcggtg accgcgctgg cgccaggaac cgcgatgacc ggaatccctc
     2941 gcgcagcggc ggcgtgcacg atggcatacc caggatccga cagcgccggg gtgccggcat
     3001 cggtcagaag tgcgactgag gcgttctcct cgagccgctg aagcaattct gccgtccgct
     3061 gccgttcgtt gtgttcatgg tagctgagca gccgtttgtc gatgccgtag tgggcgagga
     3121 gagtacgcgt gtgccgcgtg tcctcgcagg cgatgatctc gacctccttg aggacgcgca
     3181 gcgcgcgaaa ggtcagatcc tcgagatttc cgatcggggt gctgacgacg tagagggttc
     3241 ctgccactcg tgctcagcgg gcgtggagtc ccgccagcgc ccgtgcgact tcctcgagcg
     3301 ctgcctgagg ggtgtccacg acggcaatgg gcgcgcgccg gcccgcgcgg gaggcatccc
     3361 atgttcgcag cccgactacc ggtttcccaa gtcgcagcgc gtggccgagc tccgccaggg
     3421 tgccgaaccc accgccaatg gcaatgagcg cgtcgctggc cagggcgatg atgatgttcc
     3481 ggccctggcc gagtccggtc gggatggaga tgtcgacaaa tgcgttggcc tctctcgcat
     3541 cggtaccggg aaggatcccg atggtccgac cgcccgcaga ccttgcgccg cggcacgccg
     3601 cctccatcac gcctgttaga ccgccgcaga tcagaaggta ccctgcccgt gcgatcagtc
     3661 ttcccacttc ttccgccact gattgaaggg acggatccga gaattcgctt tcgccgatga
     3721 ccccgatgac gcgcgtggtc acgatcttgc gttgtgggtc ggggaatgct tcctggatcg
     3781 tgcctgcttc ttggcccctg gcgaccgccg gcgtccatcc tgcccgagca gtccacggag
     3841 gagcaggatc gcagccgctt tgtcgagcgg ctcattcatg ttgccgcact tcggcgactg
     3901 gatgcacgaa ggacaccccg cctcgcaggg gcaggcctcg atcgcggcga gcgtcgccga
     3961 catgagctca tccagcagcc cgaacccctt ttcggtgatg ccgacgcctc cgggatgccc
     4021 ttcatagata aagatagccg cactgccaag ctccggatac atcgggtagg atacgccgcc
     4081 gagatcccag cggtcgcaca tcgcaaacag cggaaggatg ccgatggccg cgtgctcagc
     4141 ggcatggatc cctcccgcca gatccaatcc ctggcgacgc acgtcgtcat cgagtgccgg
     4201 tggaatcgga aaccacagcg cggtcgtctg cagccgctcc tcggggaggt cgagcggttc
     4261 ctcgctcagg acttcgtcgc tgaagagccg cttgcgcgcg tacgcgatga cttgtgtggt
     4321 gacctcgacc tggccaaagt acgccgtggt ggacccccac ggacgctgtt tgattcgttc
     4381 gagaatctcg agatcggtcg tggtccgcgg ctgtgtgtag tagtcggcat ccgtggctcc
     4441 caccgaggcg atgcgctgct cgagatcgag ccgctgcacc agatacgact caccctgatg
     4501 gagatagatc gccccgggat gcacctgctc gaaggcgcgc gaactgtcga cggtaccgac
     4561 cagtcgtccg ctcataccgt cgacgatgcg gaacgagtta cctgaagccg accggacctc
     4621 gacctcgttg gccggatagc tccgcttggc ccaataccac cggccgagcc tgggaaccag
     4681 atcgccattc tcttgaagga tcttcacgac gtccggcaga ctcgtcccaa agagttcgct
     4741 gtcctgctcg acgagcggca cctcggccgc cgcacacttc agatggcccg cgaggatata
     4801 cggattttct ggatcgatca cggcatgctc gacgggacgc tcgaacaggt agtcgggatt
     4861 acgcatgagg tattggtcga gggcgtcttc gagcgcagcc agcaccacca gggcctcatc
     4921 ggtgccgcgc cccgcacgac ctgctcgctg ccacgtgctt gcgatggtcc ccggaaagcc
     4981 gacgagcacg gccgcatcga ggctgcccac gtcaatgccg agctccagcg cgctggtgct
     5041 gacgacccca atgaggtccc cgtggaacag acggcgctcg atcgcccgcc gttcctcagg
     5101 cagatagccg gcccgatacg ggctgatccg ggcggcgagg tccggatggt gtgtctcgag
     5161 atcgaggcga gcatagcggt agaccagctc ggtgatcttt ctggcctgtg tgaaggtgat
     5221 cgtccgcaca ccgcgccgga tcagttccgt aaacaatccc gtcgcctcgg agtacgtgct
     5281 gcgccggcgc gcccgggcct cgtcgatgat cggtgggttc cacagcgcaa accacttcgg
     5341 gccgctgggg gatccgtcaa catcgacaac ggtcaccggc agtcccagga gggcgccggc
     5401 aaactccttg gggttggcga tggtcgccga cgtgcagatg aagacaggat tagcgccgtg
     5461 catccggcag atgcgccgca gccgccggat gatgttggcg acgttgctgc caaagacgcc
     5521 ccggtagacg tggacatcat cgatgacgac atagcgcaga tttcgaaata ccgtcgccca
     5581 tttatagtgc tgcggcaaga tcccgatgtg cagcatatcg ggattcgtca gcagaatctg
     5641 ggcccgttct cgcagacgcc tgcgctggtc ttggggcgtg tccccatcgt acgttccgag
     5701 cacaaggtga ggaaatccaa attccgccag cgcatccgcc tgatcctgcg ccagcgcttt
     5761 tgtcggaaag atgtagagcg ctcgagaact tggatcctcc agcagcgttt ccaggacggg
     5821 cagattgtag cagagcgtct tgccactcgc cgtggtggtg acgacgaccg tatgctgtcc
     5881 tctgcggacg gcgccgatcg cttcgacctg atggcgatag aggcgcgtga tgccctgcgc
     5941 agcgagcgat tctcttacgg cggggaggag cggcgggcgc aactccgcgt agcgaccggt
     6001 gcggggcgga atgaaccgct catgaacgag ttgaccttca tatcccggcc tgtgcttcag
     6061 ctcgtcgaga aacgcgcgga catccatgca gtgcggaccc tacaaccatt attaggtgaa
     6121 cgacacgaat tggtcaactt gacctgacga agagcgctcg gtatcgtaga ggatgctcgt
     6181 tcgcggaagt ggcggaactg gcagacgcgc aggcctcaga agcctgtggg ggaaaccccg
     6241 tgggggttca actccctcct tccgcaccaa agtaagaggc gggatccact ctggatcccg
     6301 cctctcctta tctctcctgc aagacggtta tctgaattgg aacttcgcca tgtgcgtcgt
     6361 gccgtcggcc agctcaacca tgaacgcgcg gcattgaccc atccacgcct tctctgtctt
     6421 ccagttgaag tggaactgcc gcccgtcgta gcggaggccg gcgttgcctg gtgaacttgc
     6481 ggccataaat tcgctcgcac cgtcaccggc gcaggggatt aacgcggtct gtatccccac
     6541 aacggcgctc agcgcattga tgaggttccc gctcgcgtcc ttgatctgcc acttcacggg
     6601 aacgttggac ccagccttag cctgattgag cgcgccttcg cccacgaccg gcggcatcca
     6661 gccgagccag tgatactgga cgcgcagcga cgcagacgcg ttcgccgcac cataggtcag
     6721 cccaccgatc acgctctccg caaaagcggc ccgtacggcg tggggacctg gctccaaccc
     6781 cgaaggattg aagttccacg tggccacacc acccgacgcg gtcaccgccg aaccgagcgt
     6841 cgccccatcg accgagaagg ttatcgtggc cccatcgata ccggcgccag agtctctcct
     6901 cgtcagcgtc gctcgctgga cgaccgtgct cgatgacccc acaatcactt cggctggttc
     6961 aagatgcagg gccatgctcg aatcgatgcg cgatgcgggt cgaatgaatt gaccgaatgc
     7021 tacagggacg tttgccggga attctttgag gatggcattc gtggcccggt tgatgacgac
     7081 caccttgctg tcgtggacca gggccacgta gacttgcgct ccgtcgttgg aaattccgta
     7141 cggatggttc ccgacgtgaa catctccgat gcgcatattc gtgccggcgt cgatgaccga
     7201 cacggtgccg ctgagagagt tcgcgacgta gacgcgggcg ccgtcgggcg tcactgtcac
     7261 accaacggga ttattgccaa cggtcacggt cgcaacaaca gcgttcgtgg cgctgttgat
     7321 gaccgacact gtgcctgaat cctcgttggt gacgtagatg cggctcccgt ctgccgagac
     7381 ggctatgccg atggggctga ctcccacatc gatagaccca atggatgaga gcgttgctgc
     7441 atccatgatg gagacgctgt cgttgaagta gttgacgacg tagatcttgc tcccatcagg
     7501 gtgcacggcg atcccgcgcg ggttccccac gaagacgggc gtgacgctga acgtcgtcgc
     7561 gtcgattgcg accaaccttc cgccgttttg catcgtggtc acgaagactc ggttcccgga
     7621 cgggtgcacc gctaccccaa acggaaacat tcctaccgct gggctcgcga gcacggtgtt
     7681 ggatgccgtg tcgacgacga agagcttgcc ctggaacgcg aaatggctca cgtagacgcg
     7741 cgatccgtcg ggcgaaaccg ccaccccgta gggaacggtc cccgcgggca aggaaatcgg
     7801 ggtcactcct gtttcggtgg ccgtgtcgat aacaaggatg ttggcagaac cggagttagt
     7861 gatgtaggcg aatggcgcag cggcgacaac ggccgtcgag atcgaaagca gaagcgccac
     7921 cagcacacca agcttgccat ggcgcaacat gtttgatctt ccttgggctg agttcatggg
     7981 cgggcgcaca ttgtccgccg atgtcccgcc atgaacatac ccccaggcgg cgtggcacag
     8041 acgccatgta tgcaagtttg acagaagttt ctggtaggtt ttttggctac gtttctcggg
     8101 gggctttggc gaagcggtca aagatcaggg ccacgtgcct catatgctgg aaccagtctt
     8161 ccagcctgat gttttcgttg ggagcgtgac cgttgctatg agcataccct gagccggtcc
     8221 cgacggtggg gattccgaat tgcgccgtca gttcccacat cgggccggtc gcggccatcg
     8281 tcggcgagac caccggctcc cgaccgtaga tttcctttgc ggtttcctcc acgactcttg
     8341 ccaatgtgga gtcgacgggt gtgcgtgccg gatgctccgc gcccaacact tggacttcaa
     8401 tgtcggcgaa tccctgctgc gccaggtggc gcttcaattt ctccaggatg tcgtcgggcc
     8461 gctgctcggg aacgagtcga aagtcgatct tcgcgcgggc caaactggga agcaccgtct
     8521 tcgaaccctg tccggtgtaa cctgatacca atccacagat cgtacaggtg ggctggtaca
     8581 ggtttcgttt caccaactgc agtccggtga ctcccaggag aaactgatcg atccccagat
     8641 cgcgacgccg tacatcgtca tcgcggttgg cagcgatgcg cctgagatac tccatctcga
     8701 ccggcgtcgg ctcgaccacg tcgtcataga agccgtcgat gagaatgtgt tcgtgccgat
     8761 ccttcagcgt cgacagcgcc cagatcagtt tccacgccgg gttcggcacg accaccgcct
     8821 ggctgctgtg caggtcacgg ttggcgcctt tcgcttcaag ctcgacatac aggatgccct
     8881 tggcgcccat gtagagcccg ggattctcct ggatatcctt gcctccggct tcccagatac
     8941 acccgtcgga cttgatgaag tctttgtgct gccgcacgaa ctgatggagg tgagatgagg
     9001 agacctcttc ttcaccctcc acgacgaact tcacgttcag cggcagcgag ccgcgggtcc
     9061 gcaaccacgc ttcaaccgcg cacagcctcg ccaccagatt gcctttgttg tccgccgatc
     9121 cacgcgcgta gagtttcccg tcgcgaacgt ccgccgcaaa cggcggtgac tcccacagtt
     9181 cgagcggctc aggaggctga acgtcatagt ggttatagat gaggagtgtg cgcgtcccgg
     9241 accccgcggc cccatacacg accggcgccc cgtctgtggc gtacagctgc gggcggattc
     9301 cgatctcttc catcagcttc gcgaccatgc ccgcggtctc ttgaatcccg acgttctggg
     9361 ccgcgatgct cggctggcgg cagagccatt gcagacgctc gatgaacggc tgtttgttct
     9421 gatcgatgta gtcgaagata tcttccatga atgggagatt actgcgggtc tccttttacg
     9481 tcctccagtc cagccgcctg agcgacatag gcgacaaaat gcggctccgg ctgcgcgccg
     9541 acgaattccg tgctgtcatt gatgacgatc ttcgggacgg cgtagacgtt gtagcgctcg
     9601 gcgagatccg gaaactcgct ggcctcgacg atgtcggtag agaccttggg gctgtcgatc
     9661 gcgaagagag cggccagcct ggccgcacgc gggcagtgcg gtcaggttgg tgtgacaaag
     9721 acctgaatgt gggcgtcgtt cttcaaccgg ctcagaatct cacgggtttc gtcagcaatc
     9781 ggaggtttgc cctgggagac tgcgatgatc aggtccagaa gcgtggcgaa ctcgtagccg
     9841 ccggggatgc caaaataccg aatgccgaaa tcctgagcgc cgatcacgac ggtcgcgggg
     9901 gttcggctaa tcccgaacgc gccggccgac tcgggttcgg ccgcgagact gtgcacattg
     9961 agcgtgattt ttggtgagag agcggcgact tccgccagga tctggcgcgt gtattcagcc
    10021 atttcactgt cctgcccggg aagcaccagc ccgcccgtgc tcggcgtgaa aaactcgagc
    10081 gtgaccggat ggtcgaggtc attgtcgaat cgtttcctga caagcgcggc gtcctggtcg
    10141 ttgaggaacg gcatgatacg attactataa caggatccct catggccgcc gagtttgcga
    10201 ttgatttcaa agtcctggag catctccgcg ggtaccccga cgagctcaag cgctattcga
    10261 atctcatcaa gcaggtgcac ccgcgcggca tgtccgcggt ggagtttctc ctcaagcgcc
    10321 ccagcgccgc cgatgacttc gtcgcccagg tgtgccggct ggtccgcgac ggcgaggaag
    10381 tcatgagcgt ggtcgaggcg gcggagaccg cctctctaga gccaagagcc ttcctcgatg
    10441 gggtggcttc cgatccggaa tttccacaac cgttgtttcg gaaagaccac aaagcggtgt
    10501 ggcggcgggc cgacgtcgag gccttcttag cgcgccatac agatcgcaat gggtaagctg
    10561 cgcctcaggg tggtctgcaa agagtgcggg cgggagttcg atactccgct ggttctggat
    10621 cgaaagagct ttgcgaaggg tacgctcgct gcgaactacc atacgtgccc gcactgcggc
    10681 gagcgtctaa cgtacaggaa ggcagattac ctcgtcagcg aagagattcg cgagccccgg
    10741 taattcagcg gcgccggcgc tgctcgacga tcaggcgcaa ttcctcgagc attcggatgg
    10801 tttccacttc gaaggtctcc gtgatcagcg caaactgggc gcgctgttcg agggtcagcg
    10861 cggcgttgat ctcacgaagg attccctggc gcctgaattc gatctgcccg cggagctggt
    10921 cgagttccgt gatctttgcc tggatctctt cctcattccg cggttgctgc tgcatcaggt
    10981 cgcgtagctc ccggtgcacc gtgatcttcc ggcggccgag ctcgatgcgg agctccttca
    11041 gagccttgat gcggggaaag atccgagcga tctgcggctc ggagagattg agctcatcca
    11101 ccaaccgcca gacgagcagt gcttcaacga tgcgctcggc tctcgggtcg cgcggtccac
    11161 ggcctgcagt cgcgggaacg gagagcgcca ccagagcggc cagtagcacc accgccgccc
    11221 aacgcgctgt actgctcgtt cggctcg
//
LOCUS       DBNGSB010000136         3467 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_248903, whole genome shotgun sequence.
ACCESSION   DBNGSB010000136 DBNGSB010000000
VERSION     DBNGSB010000136.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_248903"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..140
                     /locus_tag="AC159B_03765"
     CDS             <1..140
                     /locus_tag="AC159B_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009502552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIR3376899.1"
                     /translation="FPQNRMTDHRIGLSLHRLETILDGDLHEVTAALADAEAKALATA
                     T"
     gene            167..1054
                     /gene="prmC"
                     /locus_tag="AC159B_03770"
     CDS             167..1054
                     /gene="prmC"
                     /locus_tag="AC159B_03770"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIR3376900.1"
                     /translation="MPTLREAYLLGVEHLRASGSVEPAIEAEVLLRHALRWDRTALYT
                     RWETALESEQFTRYQGLLESRAAGRPVHYIVGEREFMGLMFAVDQRVLIPRPETEILV
                     EHVINRFRGSPEPQTPNPEPRQLVIVDVGTGSGCIAVSLAHHLPPAHVYATDISEAAL
                     QVAKENARRHGVAERVVFLAGDLLTPLPRQLLGNIDVIAANPPYVPAGEAPMLAREIR
                     DFEPAVAIFAPGEATSVHTRLIEHAPRWLRSGGLLTMEVGQADTVRASIIRDGRYGDV
                     QVLPDLGGTPRVVSARTQK"
     gene            1116..1793
                     /locus_tag="AC159B_03775"
     CDS             1116..1793
                     /locus_tag="AC159B_03775"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR3376901.1"
                     /translation="MSYYNLTFSNLATLEQRQLPILKAWSGDIPRRAVETLSGGGVIV
                     FPTDTVYGVGCLIDNDDAVRRVYALKNRPQTESLPVLLADVAQLDEYASDVPAVARRL
                     AGRFWPGALTLVVRRSERVPSLVAGGGGTVGLRVPDHPVPRALVQKAGTPIVGTSANT
                     HGMPSPVTAQQAIFELGDRVDMVLDGGRVPGGRESTVVDVTTDAPRVLREGAIAAREI
                     ERTVARA"
     gene            1790..2263
                     /gene="rpiB"
                     /locus_tag="AC159B_03780"
     CDS             1790..2263
                     /gene="rpiB"
                     /locus_tag="AC159B_03780"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HIR3376902.1"
                     /translation="MKIAIASDHAGFRLKEDVKLLLRELGHEVKDFGTHSEDPVDYPD
                     FISQAAEAVGRGEFERGIALGASGNGEAMVANKLPGVRCALCHDVTTARLARAHNDAN
                     MLALGQRIIGSEVAKDIVRAWLETPFDGGRHQRRVEKIAALEARQLENGRKATTS"
     gene            2238..2900
                     /gene="upp"
                     /locus_tag="AC159B_03785"
     CDS             2238..2900
                     /gene="upp"
                     /locus_tag="AC159B_03785"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010677203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HIR3376903.1"
                     /translation="MGERRQQAKLPAGKVYVFDHPLILHKVSILRDKRTGHKEFRELV
                     EELAMLMAFEVTRTHPTREVEIDTPLAATKGRSIAGQEIAVIPILRAGLAMETGITRL
                     IPTARVGHVGIYRDPESLEPVTYYSKLPADIADREAMIVDPMLATGGSTVACIDTLKQ
                     RGCRTIKVMAIISAPEGIKKVHGAHPDVEIYTAAIDSHLNDHGYIVPGLGDAGDRLFG
                     TK"
     gene            2900..3361
                     /locus_tag="AC159B_03790"
     CDS             2900..3361
                     /locus_tag="AC159B_03790"
                     /EC_number="3.5.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF025793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004132 - dCMP deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006220 - pyrimidine nucleotide metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009972 - obsolete cytidine deamination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxycytidylate deaminase"
                     /protein_id="HIR3376904.1"
                     /translation="MSRPSWDEYFMRMAHLASTRSTCLRRKVGAVIVKDRMVLTTGYN
                     DTPRGVRNCGEGGCARCAGDAPSGTSHDTCLCIHAEMNCVIQAAYHGVSVAGGTVYCT
                     YLPCLTCAKILLNVSIKRIVFEGQYPDPLSLELLTNADVELVRYAEEGFLG"
BASE COUNT          685 a         1005 c         1105 g          672 t
ORIGIN      
        1 actttccgca gaaccggatg accgatcacc gcatcggcct gtcccttcac cggcttgaga
       61 cgatcctgga tggcgatctc cacgaggtga cggcagctct ggcagatgct gaggcgaagg
      121 ccctcgcgac ggcgacctga cagtcgtcag acggcagtct tttgatatgc cgacgctccg
      181 ggaagcttac ctcttaggtg ttgagcatct acgcgcctcc gggtcagtcg aacctgcgat
      241 cgaggccgaa gttcttttac ggcacgcgct gcggtgggac cggacagcgc tctatactcg
      301 ttgggaaacc gcgttagagt ctgaacagtt cactcgttat caaggattgc tggagagccg
      361 ggccgcgggc cggccggtgc actacatcgt cggcgagcgt gaattcatgg gcctgatgtt
      421 cgccgtggac cagcgagtgc tgatccctcg gccggaaacc gagattctgg ttgagcacgt
      481 cataaacaga tttcgtggca gtcccgaacc ccaaaccccg aaccccgaac cccgtcagtt
      541 agtgatcgtc gacgtgggga cgggcagcgg atgcatcgcg gtcagccttg cacaccatct
      601 tccgccggca cacgtgtacg ctacagatat ctccgaggcc gcgttacagg ttgcgaaaga
      661 gaacgcaagg cggcacggtg tggctgagcg cgtcgttttt cttgcaggcg atctcctcac
      721 cccgcttccc cggcagctac tggggaacat agacgtcatt gccgccaacc cgccgtacgt
      781 gcccgcgggt gaggcgccca tgctggcgcg agagattcgg gactttgagc ccgccgtggc
      841 catatttgct ccgggggagg cgaccagcgt ccacacgcgg ttgatcgaac acgcgcctcg
      901 ctggctgcga tccggcggtc tgctgacgat ggaggtcggt caggccgaca ccgttcgcgc
      961 ctcgatcatt cgggatggcc gatacggtga cgtgcaggtc ctgcctgatc ttggggggac
     1021 ccctcgcgtc gtgtcggcgc gaacacagaa gtagtaaaac cgcaagatct gggagactca
     1081 gaagactcgg aagaggcgcg gcgaattagt atgcgatgtc ctactacaac ctcacattct
     1141 ccaatctcgc aactctcgag caacgtcaat tacccatatt gaaagcttgg tctggcgaca
     1201 ttccccgccg tgcggtggaa acgctgtccg gtggaggggt gatcgttttt cccaccgaca
     1261 cggtgtatgg tgttgggtgc ctgatcgata atgatgatgc agtgcgacgc gtctatgcgc
     1321 tcaagaaccg gccccagacg gaatcgctgc ctgtgctgct tgccgatgtg gcacaacttg
     1381 atgagtatgc gagcgacgtg cccgctgttg cgcggcgcct ggctggccgt ttctggcccg
     1441 gggcgctgac gctcgtggtg cggcgctcag aacgagtgcc atccctcgtt gcaggcggag
     1501 gggggaccgt gggactgcgc gtacccgacc atccggtccc ccgagccctg gttcagaagg
     1561 cgggcacccc catcgtcggg acgagcgcaa acacgcacgg aatgccatca ccggtgactg
     1621 cacagcaggc gatatttgaa ctcggcgatc gagtcgacat ggtcctcgac ggcgggcgag
     1681 ttcccggagg gcgcgagtcg acggtcgtgg acgtcaccac cgacgctccc agggtgctgc
     1741 gcgagggggc aatcgcagca cgggagatcg agaggacggt ggcgcgggcg tgaagatcgc
     1801 gatcgccagc gatcacgcag gcttccggct caaggaagac gtgaaactgc tgctacgaga
     1861 attgggacac gaggtaaagg atttcggaac gcactccgag gaccccgtag attatccgga
     1921 cttcatttcg caggcagctg aggccgtggg gcgaggcgaa ttcgagcgag ggattgcgct
     1981 gggggcttca ggcaacggag aggcgatggt cgccaacaag cttcccgggg tccgctgcgc
     2041 cctgtgtcac gacgtgacga cggcgcggct tgcccgtgcg cataacgacg ccaatatgct
     2101 ggcgctcggt cagcgcatca tcggcagtga ggtggcgaag gacattgttc gcgcgtggct
     2161 tgagacgccg tttgacgggg ggcgacacca gcggcgagtc gagaaaatcg cggccctcga
     2221 agcgaggcag cttgagaatg ggcgaaaggc gacaacaagc taaactgccg gcgggaaagg
     2281 tctacgtgtt tgatcacccg ctgatcctgc acaaggtttc catccttcgc gacaagcgca
     2341 ccggacataa agagttccgc gagctggttg aagagctggc gatgttgatg gcgtttgaag
     2401 tcacccgcac gcaccccacg cgggaagttg aaattgatac cccgttggcg gcgacgaagg
     2461 ggcgcagcat cgccgggcag gagatcgccg tcattcccat cctgcgggcc ggactggcca
     2521 tggagacagg gattacgcga ctgatcccca cggcccgcgt cggccacgtc ggcatctacc
     2581 gggaccccga gagtttggaa cccgtcacgt attactccaa attgccggcc gacattgccg
     2641 accgtgaagc aatgatcgtg gatccgatgc tggccacggg cggatcgacg gttgcatgta
     2701 tcgacacact gaagcagcgc ggctgcagga cgatcaaagt gatggcgatt atctccgcgc
     2761 cggaaggcat caagaaagtg cacggcgcgc atcccgacgt cgagatctat acggccgcga
     2821 tcgacagcca cctcaacgat catgggtata tcgttcctgg cctcggggac gctggagata
     2881 ggctcttcgg gaccaaatga tgtccagacc atcttgggat gagtacttca tgcggatggc
     2941 acacctcgct tcgactcgct caacatgctt acggcgtaaa gtaggagcgg tgattgtcaa
     3001 ggatcgcatg gttctaacga cagggtacaa cgacactccg cgaggtgttc gcaactgtgg
     3061 agagggcgga tgcgctcgtt gtgcagggga tgcgccctcc ggtaccagcc atgacacctg
     3121 tttgtgcatt cacgctgaga tgaactgtgt cattcaagca gcctatcatg gtgtttcagt
     3181 ggctggcgga acggtgtact gcacgtatct cccatgcctg acgtgtgcga agatccttct
     3241 aaacgtcagt atcaagcgga ttgttttcga aggtcagtat cctgatccac tgtcattgga
     3301 gcttctcaca aatgctgacg tggaactggt ccgctacgcg gaagaggggt tcctaggcta
     3361 ggcatcggga gctcttcgat ccttctgcat tctcgcgctt ccgcgcttcc gcgtttccgc
     3421 gcttccgtct tcgtgtcgtt gtgccatcca cggcggcttc ccaagat
//
LOCUS       DBNGSB010000137         8303 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_1935768, whole genome shotgun sequence.
ACCESSION   DBNGSB010000137 DBNGSB010000000
VERSION     DBNGSB010000137.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 8303)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8303)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8303
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_1935768"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="AC159B_03795"
     CDS             complement(<1..245)
                     /locus_tag="AC159B_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376905.1"
                     /translation="MSGTHRDVERDACGVGFVADLHGRPTHDIVAHALHCVTNLSHRG
                     AAAADGKTGDGAGVLTQLPYALLKRDLNTSIADADLAA"
     gene            579..1406
                     /gene="proC"
                     /locus_tag="AC159B_03800"
     CDS             579..1406
                     /gene="proC"
                     /locus_tag="AC159B_03800"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HIR3376906.1"
                     /translation="MLSGTVGFIGAGNMADALIRGMIGRGILSQDRIWATNRSNRARL
                     LGLQERYGVHTTPVKPPLLNASDVLIVAVKPKDMAEVLGEIHGAVRQDQLVISVAAGV
                     SLRAIEDQLSGVPVVRAMPNTSAAVGASVTALAPGRSATDAHLHVTEELFRAVGEVLT
                     VPEETMDVVTGLSGSGPAYVYRFIEALIAAGTELGLTEELAKQLAVHTLVGAARMLSE
                     PNADSVELRRRVTSPGGTTQAGLTALEERGFASAVKEAVRRAAERAAELSQEHSKKA"
     gene            complement(1587..3257)
                     /gene="ilvD"
                     /locus_tag="AC159B_03805"
     CDS             complement(1587..3257)
                     /gene="ilvD"
                     /locus_tag="AC159B_03805"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIR3376907.1"
                     /translation="MTTLLRHRSSALLDGPDRAPARAYFKAIGFTDDDLARPLIGIAN
                     TWIELMPCNYHLRRLAERVKAGVRAGGGTPVEFNTIAISDGISMGTEGMRTSLVSREV
                     IADSIELVARGYLLDGLVAISGCDKTIPGAVMALARLNLPSLMLYGGSILPGEFEGRP
                     VTIQDVFEAVGAYHSGAMSEARLRALEASACPGPGACGGQFTANTMAMVFEIMGISPT
                     GSSDVPAVDAQKDDVAFEAGRLVMRVLRDDIHPRAFITRASLENAIAAVAASGGSTNA
                     VLHLLAVAAEGGVALDINDFDRISARTPLLADLKPGGRFTAVDLHRAGGVRLLARRLL
                     EAGVLHGDAPTVTGRSIGSEARDASETPGQQVVSSAAAPLRAWGGLAILKGNLAPEGC
                     VVKVGHERLYHRGPARTFDNEEDAFAAIRDREIAHGDVLVIRYEGPVGGPGMREMLAV
                     TAALVGTGLGESVALVTDGRFSGATRGLMVGHLAPEAARGGPIAAVRDGDTIIIDVSR
                     RALEIELSAEEMNRRLAQWTPPPPRYPGGVMGKYARLVTSASLGAVTQ"
     gene            complement(3254..4339)
                     /gene="leuB"
                     /locus_tag="AC159B_03810"
     CDS             complement(3254..4339)
                     /gene="leuB"
                     /locus_tag="AC159B_03810"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008340215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HIR3376908.1"
                     /translation="MPERYRVAVLGGDGIGPEVIREAVKVLSAASRRFGIEWAFVEGL
                     IGGVAIDATGSPLPDETLALCRSSEAVLFGAAGGPRWDALPGGARPERGLLRLRQELA
                     VYANLRPVRLMVEGSSPLRAQVVAGMDLVIVRELTGGLYFATPRGRSGDSALDTLRYT
                     RDEIARVVRVALRLARSRRRALTSVDKANVLESSRLWREVVEEEARAFTDVAVRHLLV
                     DTAAMELVRAPATFDVIVTENMFGDILSDEAAGVVGSLGLLPSASLGDRAPFLYEPVH
                     GSAPDIAGMGIGNPVGAILSAAMLLRYSCDLPSAAAAVEAAVAAVLARGVKTPDLGGS
                     SSTVDVGDRIAAAVEEGQPVGLGELRS"
     gene            complement(4332..4472)
                     /locus_tag="AC159B_03815"
     CDS             complement(4332..4472)
                     /locus_tag="AC159B_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376909.1"
                     /translation="MSPLQQPRRSEEPKTRTEPAENDPEQENTEEGWVVTQIQESFES
                     RA"
     gene            complement(4510..5013)
                     /locus_tag="AC159B_03820"
     CDS             complement(4510..5013)
                     /locus_tag="AC159B_03820"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HIR3376910.1"
                     /translation="MAMRGTAHKLGHHVDTDVIIPARYLVTNDPAELARYCFEDLDPT
                     LRARIRPGDIIVAGENFGQGSSREHAPIAIKALGIPCIVASSFARIFYRNAFNIGLPL
                     VESADAYAGIEEGHDIEVDLSAGRVDNHSTAATFISAPVPDFMQRLLDAGGIVGYARM
                     QLAQRKR"
     gene            complement(5018..6298)
                     /locus_tag="AC159B_03825"
     CDS             complement(5018..6298)
                     /locus_tag="AC159B_03825"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIR3376911.1"
                     /translation="MSMTITEKILAAHADADHVRPGQILDCRVDFVFANDITAPLAIQ
                     EFLKMDTPQVFDPDRVAFVFDHYVPSKDIASAQQCKITRDFARAHDLPHFYDIGRSGI
                     AHVLLAEEGFVGPGDVFVGADSHTCNQGALGAFATGVGSSDLAAAMALGRLWLRVPET
                     VKYVLAGTLRPWVTGKDLILAIIGRIGVDGARYGAMEFAGEVLAQLSMDERFSITNMA
                     VEAGAKNGIMEPDDVTLAWFTPRARRPSTVYRSDPDATYADVRDIDVSALEPVVSVPS
                     SPGNVVPVREVAGVKVDQCFIGTCTNGRIDDLRMAAAVLAGRKVNLHTRLLVIPATPR
                     VYQQALEEGLVQIFLDAGGAISTSTCGPCIGGHMGVLGEGEVCVSTSSRNFIGRMGHR
                     SSQVYLSNAGVAAASAVLGRLAHPDEVVRVPVPA"
     gene            complement(6295..7479)
                     /locus_tag="AC159B_03830"
     CDS             complement(6295..7479)
                     /locus_tag="AC159B_03830"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HIR3376912.1"
                     /translation="MTDRVVIFDTTLRDGEQSPGFSMSAGEKLELARQLARLGVDVIE
                     AGFPVSSPGELEAVQQIAAGVRGPAICALARTDPRDIDLAWEAVRGAEHPRIHTFIAT
                     SPIHMERKLRMTPDEVRLHAQRAVAMARSLVPDVEFSAEDATRSDREFLCRIFDDAIA
                     AGATTINIPDTVGYALPEEFGALVRTVLERVPGMDRVAVSVHCHNDLGLATANTLAGL
                     AAGARQVEGTINGTGERAGNVALEEVIMAVATRRESLGLSTAIDTTQLHPTSRLLCAL
                     TGIDVQPNKAIVGANAFAHEAGIHQHGVLMDRRTYEIMTPESIGLPHNRLVLGKHSGR
                     HAFQKVLEDEGVALGSEALDVIFARFKDLCDRKKRVTLEEIVALVEEMRTTPAASGGG
                     AR"
     gene            complement(7490..>8303)
                     /gene="ilvC"
                     /locus_tag="AC159B_03835"
     CDS             complement(7490..>8303)
                     /gene="ilvC"
                     /locus_tag="AC159B_03835"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HIR3376913.1"
                     /translation="VEGADVVSILAPDHVQKAIYDREVARAFRPGAALLFAHGFNIHF
                     GQIQPSPGVDVIMVAPKSPGHRMRELFTEGASVPALLAVHQDATGHAKEIALAYARGL
                     GCTKAGVIETTFREETETDLFGEQAVLCGGVSELIKAGYETLVAAGYQPEIAYFECLN
                     EMKLIVDLLYEGGFSYMRYSVSDTAEFGDYSRGRRVVDSHVRATMQQILQEVQDGTFA
                     REWVLENQAGRPMFSARRRAETEHPIEVVGRRLRAMMPYLKRPTRQPVGTAS"
BASE COUNT         1454 a         2787 c         2611 g         1451 t
ORIGIN      
        1 gccgcgagat cggcatccgc gatcgaggta ttgaggtcgc gcttgagcag cgcgtaaggg
       61 agttgcgtca ggacgcccgc cccgtcgccg gtctttccgt ccgcggcggc ggcgccccga
      121 tggctgaggt tggtcacgca gtgaagtgca tgcgccacga tgtcgtgggt cggtcgaccg
      181 tggaggtctg cgacgaaccc aacgccgcag gcatcgcgct caacgtctcg atgcgtacca
      241 ctcatctgtc gaatctgttt gacccccagc ggatgacgac aaatgcaaaa gcccgggagg
      301 gtcatccctc tccctgcctc gaggcaaggc ccggaaggcc tgaaccttcc gggatgagga
      361 ccctccccct cgggcttctc tcccgacggt gtggccgata tcgccggcct gcgccgctag
      421 gaccagtccc gcacccacga cggtgcggcg gaacgctagg cgcacttcct cagaaatgcg
      481 tttgtgtttt ctacggttta acagaaaaca tccgaggtgt caagcaaaag cccttggatt
      541 gcttgttttc cgcatctcca actgttagag tacgcgatat gctctctgga accgtcggat
      601 tcattggcgc cgggaacatg gccgatgccc tcattcgggg catgatcggc cgcggcattc
      661 tctcgcagga tcggatctgg gctacgaatc gatcgaaccg ggcccggctc ctcggcttac
      721 aggaacgtta cggggtccac acgacccctg tgaagccccc actcctcaat gcatcggacg
      781 tgctgatcgt ggcggtgaag cccaaggata tggcggaagt actcggggag atccacggag
      841 ccgtccgcca ggatcagctc gtcatttccg tcgcggccgg agtttcactg cgggccattg
      901 aggaccagct cagcggcgtc cctgtggtcc gcgccatgcc gaatacatcc gccgcggttg
      961 gggcgtcggt gacggcgctt gctccgggac gttccgcgac tgatgcccat ttgcatgtca
     1021 ccgaggaact gtttcgagcc gttggcgaag tcctgacggt ccccgaggag acgatggatg
     1081 tagtgaccgg tttgtcaggg agcgggccgg catatgtcta ccggttcatc gaggccttga
     1141 tcgccgccgg caccgagctg ggccttaccg aagaactcgc aaaacaactc gcggtgcaca
     1201 cactggtcgg agccgcgcgc atgctctccg aaccaaatgc ggactcggtc gaactgagac
     1261 gccgtgtgac ctcgccggga ggaacgactc aggcgggtct gaccgcgctc gaagagcgcg
     1321 gatttgcgtc tgcggtaaag gaagcggtcc gtcgagccgc cgagcgcgcg gctgagctct
     1381 ctcaagagca cagcaagaaa gcctagcggt cagccttcgg ttcgtacgtt tccttccgct
     1441 cggtcttctc cgaactcact gcggcagtgc gcacgcagtc caggcagagc cacttcgtgc
     1501 cacggccgaa ccactggact ttcacaagga cggtggcgtt actcaatttt ccgcacgcct
     1561 gacactgact catcgacgca catgcctcac tgtgtcactg ccccaagact cgccgacgtg
     1621 accaggcgcg cgtatttgcc catgacgcct cccggatacc gtggtggcgg gggcgtccac
     1681 tgagccaggc ggcggttcat ctcttccgca gaaagttcta tctcaagggc gcgccgggag
     1741 acatcgatga tgatggtatc tccgtcgcgc accgcggcga tgggaccgcc ccgcgccgct
     1801 tccggtgcga ggtgtccgac catcaatccg cgcgtggcgc cgctgaaccg gccgtcggtg
     1861 acaagggcaa cggactctcc cagcccggtg cctaccaggg ccgccgtcac tgcgagcatc
     1921 tctcgcatgc cgggaccgcc caccggcccc tcatagcgga tcaccaacac atccccgtgc
     1981 gcgatctcgc ggtcgcggat ggccgcaaat gcgtcctcct cgttgtcgaa cgtgcgtgcc
     2041 gggccacgat ggtagagacg ctcatgcccg actttcacga cacatccttc gggcgccagg
     2101 ttgcctttga gaatagccag gccgccccac gctctgagag gagcggcggc tgaactgacg
     2161 acttgctgtc cgggtgtttc gctcgcgtcc cgggcttcgc tcccgatcga ccggcccgtc
     2221 acggtcggcg catcgccgtg aagcacgcct gcttccaaga gccgcctggc caacaggcgc
     2281 acgcctccgg cgcggtggag gtcgaccgcg gtgaacctgc cgccgggctt gaggtcggcc
     2341 agcagcggcg tacgcgcact gatgcgatcg aaatcgttga tgtccaacgc aactccaccc
     2401 tccgccgcga cagcgagcag atgcagcacc gcgtttgtcg atccgccgga cgccgccacc
     2461 gcggcgatcg cgttctccag cgacgcgcgg gtgatgaacg ctctgggatg aatgtcatcg
     2521 cggagcacgc gcatgaccaa ccggccggcc tcgaacgcga cgtcgtcttt ctgcgcatcc
     2581 actgcgggca catcactgct gcccgtcgga gagatcccca tgatctcgaa gaccatggcc
     2641 atcgtgttcg cggtgaactg cccgccgcag gcaccgggac ctggacaggc gctcgcttcc
     2701 agcgcccgca gcctggcttc actcatggca ccgctgtgat aagctcctac ggcttcaaag
     2761 acatcctgga tcgtcaccgg ccgtccctca aactcccccg gcaggatcga tccgccgtag
     2821 agcatcagcg agggcaggtt caaccgggcc aaggccatga cggcgccggg aatcgtcttg
     2881 tcgcagcccg agatcgctac gagtccgtcc agcagatatc cacgcgcgac gagttcgatc
     2941 gaatcggcga tgacctcgcg gctgacgagt gacgtccgca tgccttcggt gcccatcgag
     3001 atgccgtcgg aaattgcgat ggtgttgaac tccacgggcg tgccgccgcc cgccctgacg
     3061 ccggccttga cgcgctccgc gaggcggcgc aggtggtaat tacagggcat gagctcgatc
     3121 caggtgttgg caattccaat cagcggacgc gcgaggtcat cgtcggtgaa tccgatcgct
     3181 ttgaagtacg cgcgcgccgg cgctcgatcg ggtccatcaa gcagtgcgct gctcctgtgt
     3241 cgaagcaaag tcgtcatgac cgcagctccc ccaggccgac aggttgtccc tcttcgaccg
     3301 cggctgcgat gcggtcaccc acgtccacgg tcgacgagga cccaccgagg tccggtgtct
     3361 ttactccgcg ggccagcacc gctgcgacgg cggcttcgac cgcagcggca gcggacggaa
     3421 gatcgcacga gtaccggagc aacattgcgg cgctgagaat cgcgcccacg ggattgccga
     3481 tccccatacc ggcgatgtca ggcgcggatc catgcacggg ctcatagaga aacggcgcgc
     3541 gatcgccgag acttgccgac ggcagcagcc cgagcgaacc cacgacacct gcagcttcgt
     3601 cgctcaggat gtcgccaaac atgttctcgg tcacgatcac gtcgaacgtt gccggcgcac
     3661 ggacgagttc catcgcggcg gtatccacga gcagatggcg cacggccacg tcggtaaacg
     3721 cccgcgcctc ctcctccacg acttccctcc agagccggga tgactccagc acgttcgctt
     3781 tgtccacgga ggtgagcgcg cgccggcgtg atcgggcgag gcgcagcgcc acgcgcacga
     3841 cgcgggcgat ctcatctctg gtgtatcgca gcgtgtcgag tgcagaatcg cccgaccggc
     3901 cccggggtgt cgcgaagtac agaccgccgg tgagctcgcg cacgatcacc aggtccatgc
     3961 cggccaccac ctgcgcccgc aggggcgaac ttccttctac catcaggcgc acgggccgga
     4021 ggttggcgta gactgccagc tcctgccgca gtcgaagcag tccacgctcg gggcgcgcgc
     4081 cgccaggcag cgcatcccat cgcggcccac cggcggctcc aaacaacacc gcctcggagg
     4141 accggcacag cgcgagtgtc tcatccggca acggcgagcc ggtcgcatcg atggccacgc
     4201 cgccgatgag cccctcgaca aaggcccatt cgataccaaa gcggcggctc gcggcgctga
     4261 gtaccttgac cgcttcgcgg ataacttctg gaccgatacc gtcgccgcct aagaccgcga
     4321 cgcggtacct ctcaggcacg gctctcgaag gactcctgaa tctgagtgac gacccacccc
     4381 tcttcggtat tctcctgctc cggatcattt tccgccggct cggtgcgggt cttgggctcc
     4441 tcactgcgcc gcggttgctg tagcgggctc atctcttccc tccggtacgg aatgtcgtgc
     4501 agtcttcagt caccttttgc gctgggcgag ctgcatccgg gcgtagccca cgatgccgcc
     4561 ggcatcgagc agtcgctgca tgaaatccgg aacgggggcg gagatgaacg tagcagcggt
     4621 cgagtgattg tcgaccctcc cggcggacag atcgacttcg atgtcgtgac cctcttcaat
     4681 tcccgcgtag gcgtcagctg actccacaag cggaagtccg atgttgaacg cgttgcgata
     4741 gaagatccgt gcgaaggagg aggccacgat gcacgggatg cccagggcct tgatggcgat
     4801 gggcgcgtgc tcccgcgagc tgccctgacc gaagttttcg ccggcgacga tgatgtcgcc
     4861 gggccgaatg cgcgcgcgca gcgtcgggtc aagatcttcg aagcagtatc tggcgagctc
     4921 ggccggatca ttcgtcacca ggtatcgcgc ggggatgatc acgtcggtgt cgacgtggtg
     4981 ccccagtttg tgcgccgtgc cgcgcatcgc catcgcctca ggccgggacc gggacgcgga
     5041 cgacctcatc ggggtgggcc agcctgccca acacggccga tgccgccgcc acaccggcat
     5101 tcgagaggta cacctggcta ctgcggtgtc ccatgcgtcc gataaagttg cgactgctgg
     5161 tagacacaca tacttcaccc tcgccgagca cacccatgtg accgccgatg cacgggccac
     5221 aggtcgacgt gctgatcgcg ccaccggcat caaggaaaat ctgaacgagc ccttcctcca
     5281 gcgcctgctg atagaccctg ggcgtggccg ggatgacgag gagccgtgtg tgcagattca
     5341 ctttccggcc ggctaacacg gcggcggcca tgcgcaggtc gtcgatccgg ccgttggtac
     5401 acgtcccgat gaaacactga tcgaccttca ctccggccac ctcacgcacg ggcacgacgt
     5461 tcccgggcga tgaagggacg gacacgaccg gctccaatgc ggagacatcg atgtcgcgca
     5521 cgtcggcgta ggtcgcgtcg gggtcgctgc gatacacggt ggacggccgg cgggcgcgcg
     5581 gcgtgaacca ggcgagcgtc acgtcgtcgg gctccatgat gccgttcttc gcgcccgctt
     5641 cgacggccat gttcgtgatc gagaaccgct cgtccatgga aagctgggcg aggacttcac
     5701 ccgcgaactc catcgccccg taccgagcgc cgtcaacacc gatgcggccg atgatggcca
     5761 ggatcaggtc tttgccggtc acccacggcc gcagcgtgcc ggccaggaca tatttgacgg
     5821 tctccggcac acgcaaccag agccgcccca gcgccatcgc tgctgccaga tccgatgacc
     5881 ccacgccggt ggcaaaggcc cccaacgcgc cctggttgca cgtgtgggaa tctgcgccga
     5941 cgaagacatc acctggtccg acgaaccctt cctcggccag cagcacgtgc gcgatgcccg
     6001 atcggccgat gtcatagaaa tgcggtagat cgtgcgcgcg cgcaaaatcc cgcgtgatct
     6061 tgcactgctg cgcggacgcg atgtcctttg acggcacgta gtggtcgaag acgaaggcca
     6121 cgcggtcggg atcgaatacc tgcggagtat ccatcttcag aaactcttga attgcgagcg
     6181 gcgccgtgat gtcgttggcg aacacgaaat cgacgcggca gtcgaggatc tgcccgggcc
     6241 gcacgtgatc ggcgtccgca tgtgcggcga ggatcttctc ggtgatcgtc atgctcatct
     6301 cgccccaccc ccggacgctg cgggtgtagt ccgcatctcc tcgaccagcg cgacgatttc
     6361 ctcgagggtg acccgcttct tgcgatcgca caagtccttg aagcgtgcga agatcacatc
     6421 cagcgcctcc gatcccagag ccaccccctc atcctccaac actttctgga acgcgtggcg
     6481 gccggaatgt ttgcccagca ccagccggtt gtggggcagg ccaatcgatt cgggcgtcat
     6541 gatctcgtag gtgcgacggt ccatcagcac gccgtgctgg tggatgcccg cctcgtgcgc
     6601 aaacgcgttc gcaccgacga tcgccttgtt cggctgcacg tcgatcccgg tcagcgcgca
     6661 cagcaaccgg ctcgtgggat ggagctgcgt cgtatcgatg gcggtggaga gaccgagcga
     6721 ttcgcgccgg gtggcgacgg ccatgatcac ttcttccagc gcgacgttgc cggcacgctc
     6781 accggtgccg ttaatggtgc cctcgacctg acgtgccccc gcggccagac cggccagcgt
     6841 attggccgtg gcgagtccca ggtcgttgtg gcaatgcacg ctcaccgcaa cccggtccat
     6901 cccggggaca cgctcgagca ccgtgcgtac gagtgcaccg aactcttcgg ggagcgcata
     6961 gccgaccgta tcaggaatgt tgatcgtcgt cgccccggcg gcgatcgcgt cgtcgaagat
     7021 ccggcagaga aactcccgat ccgaccgtgt ggcatcttcc gcgctgaact ccacgtccgg
     7081 gaccagggac cgcgccatcg ccacggcccg ctgagcgtga agacgcacct cgtcaggcgt
     7141 catcctgagc ttccgttcca tgtggatcgg agaggtcgcg atgaacgtgt ggatgcgggg
     7201 atgctctgcg ccgcgcaccg cttcccaggc caggtcgata tcgcggggat cggtgcgcgc
     7261 gagggcgcag atcgccggcc cgcgaacgcc ggcggcgatt tgctgcacgg cctccagttc
     7321 accgggcgag ctgaccggaa agcccgcctc gatgacgtcg accccgaggc gagcaagctg
     7381 acgcgccagc tccaatttct cgcccgcgct catcgagaat ccgggcgatt gctcgccgtc
     7441 gcgcagggtc gtgtcgaaaa tgacgacgcg atcggtcatc gatgtgctcc tacgaggccg
     7501 tgcctacggg ctggcgggtg ggacgtttga ggtacggcat catcgcgcgc agccgccgcc
     7561 ccacgacctc gatgggatgc tccgtttctg ctcgccgccg tgcgctgaac atcggccggc
     7621 ccgcttgatt ctccagcacc cactcgcggg cgaatgtgcc atcctggact tcctggagta
     7681 tctgctgcat cgtcgcccgg acatggctgt cgacgacgcg ccggcctcga ctgtagtcgc
     7741 cgaactcggc ggtgtccgag acggagtagc gcatgtacga gaacccgcct tcgtagagca
     7801 ggtcgacgat gagcttcatt tcattgagac actcgaaata cgcgatctca ggttgatacc
     7861 cggccgccac cagtgtctcg taccccgctt tgatgagctc gctgacgccg ccgcagagca
     7921 ccgcctgctc gccgaagaga tccgtttccg tttcctcgcg gaatgtcgtc tcgatgactc
     7981 cggctttggt gcagccgagg ccccgggcat aggccagggc gatctccttc gcgtggccgg
     8041 tcgcgtcctg atgaacggcc agcagcgcgg gaacactggc gccttcagta aagagctctc
     8101 gcatgcggtg gccgggtgat ttcggcgcta ccatgatgac gtcgaccccg ggcgagggct
     8161 ggatctggcc gaaatggatg ttgaatccgt gcgcaaataa gagagccgcc ccaggtcgga
     8221 acgctcgcgc gacctcccga tcgtagatgg ccttctggac gtggtccggc gcgaggatcg
     8281 acacgacgtc agcgccttcc acg
//
LOCUS       DBNGSB010000138         7406 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_251660, whole genome shotgun sequence.
ACCESSION   DBNGSB010000138 DBNGSB010000000
VERSION     DBNGSB010000138.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 7406)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7406)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7406
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_251660"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            348..716
                     /locus_tag="AC159B_03840"
     CDS             348..716
                     /locus_tag="AC159B_03840"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HIR3376914.1"
                     /translation="MNRKELGALGEAAAVRLLVRLGYKIRHRNFRCPLGELDLVAEER
                     GVLAFIEVKTRTTAEFGVPLEAVSPAKQRRLVRLATYYLAGQRMLERPCRFDAVSVVI
                     SPGGRIEAVELIRDAFVLPE"
     gene            777..2303
                     /locus_tag="AC159B_03845"
     CDS             777..2303
                     /locus_tag="AC159B_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HIR3376915.1"
                     /translation="MLAKVRTAAVQGLDAYPVDVEVDVGGGLPAFTIVGLPDTAVQEA
                     KERVRAAIRNSNYEVPSRKITINLAPADVRKEGPAFDLPMAIGILLATEQIHASGLDG
                     AMALGELSLDGGIRPVPGLLSIALAARAGGMHTLIAPAVNAQEAALVEGLQVYAVRSL
                     FQVIRHLEGEEVLTPVRSPPHSGAGADYPVDFAEVKGQAYARRALEIAAAGGHNVLMV
                     GPPGAGKTMLARRLPTILPPLIWDEAIDVTRIYSVAGLLNGRGPVHERPFRSPHHSTS
                     SAALVGGGTVPRPGEVTLAHHGVLFLDELPEFHRNALEVLRQPLEEGFVTVARVQSTV
                     TFPARFMLVAAMNPCPCGYHGDGLRECLCTPSQIARYLGRVSGPLLDRFDLHIEVPRV
                     PSGDLVTTLSAEPSHEIRTRVLHARARQQKRLDSPSGCNAGMTGRSLRKYCRLDEDGR
                     TFLRAAIERLGLSARAHDRILRLARTIADLEAVDALTTAHIAEAVQYRAFDRPLRLLA
                     "
     gene            2321..3190
                     /gene="dprA"
                     /locus_tag="AC159B_03850"
     CDS             2321..3190
                     /gene="dprA"
                     /locus_tag="AC159B_03850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HIR3376916.1"
                     /translation="MGDNDGIYVVARDDGEYPAALRPIRDAPRELFVRGTLLPQDEIA
                     VAIVGARRASEYGKAVARRLAADLAACGVTIVSGMARGIDGAAHQGALDAGGRTLAVL
                     GCGPDVVYPTVHAQLMRRIICSGAVISEFRPETPPRPEQFPQRNRIISGLAKGVVVVE
                     GRVGSGSLITADFALEQGREVFAVPGNIFEETSWAPHHLVSQGARLVASADDILDELK
                     ISHRSAPQRAAVSLDDGERAVWAQLSLVPQHVDRIAILSGQPVALTSRILLSLEAKGL
                     VGALAGQRYVKKV"
     gene            3197..5464
                     /gene="topA"
                     /locus_tag="AC159B_03855"
     CDS             3197..5464
                     /gene="topA"
                     /locus_tag="AC159B_03855"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018883417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HIR3376917.1"
                     /translation="MPKRKVSTDGAAPKKRMKAPLPEVPAAPEPQLGESANGESRAAA
                     GKPRSLVVVESPTKARTLKKLLTRRYDVFASMGHLKDLPRSQLGVDVKNGFTPKYIVV
                     KGKGSILKELKEAAKQAPVVFMATDPDREGEAISWHLADALRSVNPEIKRIEFHEVTK
                     DAVRRALANPRDIDLNLVNAQQARRILDRLVGYKLSPLLWRKIRGGLSAGRVQSVAVR
                     LVVEREREIESFVPQEFWSLLARLRKDDQQIEARLYGKNGTRYGSPGEEDVTVLRTSD
                     SVEAIVRDLGGATYTVGEVRRKDQFRNPAPPFTTSTLQQEANRKLGYSAARTMNVAQQ
                     LYEGVDLGPEGTVGLITYMRTDSVRVSEGAQREAREYITQTYGDSYVPDVPRVYRSRR
                     EAQEAHEAIRPTAVRRAPDRVKPFLRSDQYKVYKLIWERFLASQMASAIMDTLSVDIH
                     AGSYLFRATGSRVKFPGFLAVYREGTDANGEEKEGWLPELATGDRLTLMELAPHQHFT
                     QPPPRFTEASLVKALEERGIGRPSTYAPTIETIKRRGYVQSTSRRLQPTDLGVLVNDM
                     LVEHFGDVIDYDFTATMEEELDQVEEGRLDWKKVVGDFYKPFEHDLVIAEKKIEEIKM
                     PVVEIGEACPECGRPLVRKHGRFGEFIACSGFPECKYTRPLGIGVPCPKCKVGEVVER
                     RSRRGRTFFGCSTYPACTFTSWDKPTEKVCPRCGKNVLGIHQTARKMTLRCLDRTCGY
                     TEPVTPEPAEEPVPR"
     gene            5480..6361
                     /locus_tag="AC159B_03860"
     CDS             5480..6361
                     /locus_tag="AC159B_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HIR3376918.1"
                     /translation="MQRDIDGFLAALEGERGVSSHTLTGYGRDLHHFARFLAAERVTA
                     WSAVTPQIARRYVASMDRRYARSSVARRLSALRTFYKFLYREGAVSKNPLVLVGTPKQ
                     PRRLPKFLTPHEVAALLAAPNLATPIGLRDRALLEVLYAAGLRVGELTSLRSGDLPSG
                     DEIRIVGKGRRERIALLTRSALEALRAYVEEGRPSLARRRDIDAVFLNARGGALSDRG
                     VRLIVDRHIRKVASDRRISPHALRHTFATHLLDGGADLRSVQELLGHRSLATTQIYTH
                     VSRDWLKRVYDRAHPRA"
     gene            6518..7363
                     /gene="rpsB"
                     /locus_tag="AC159B_03865"
     CDS             6518..7363
                     /gene="rpsB"
                     /locus_tag="AC159B_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HIR3376919.1"
                     /translation="MPVVTMKQLLEAGVHFGHQTRRWNPKMARFIFTERNGIHIIDLQ
                     KSVPMIEEAYATVRDAVANGGTCLFVGTKKQAQDAIREESTRASQPYVCQRWLGGMLT
                     NFQTIRKRVERLREIEDMRDRGLMDVLPKREQARLLEELARLEKYLGGIKAMSQLPSA
                     VYVVDTRKEHIAIAEARKLGIPVVAIIDTNCDPDEVDYPIPGNDDAIRAVRLITSRIA
                     NAAMEGLEERRKREVVEETGEPLAASEAPEAESFDEKAVPAEAGIEALPEPVPTETLV
                     ENEVS"
BASE COUNT         1387 a         2291 c         2442 g         1286 t
ORIGIN      
        1 tgcactggtg ccttgtgacg taagttgcaa ttgatggatc agggcgccgc ccgtaaacac
       61 atcgaaggac tgtcctacca aagccgatgc cggccaaggc agcaacagga cgaacacgag
      121 cgaagccaga tagcgcatct cacgacctcc taccgcagga atgccacaac agtcgtaggc
      181 gcagactcag atctctcacg catctttccg cgacctgact gcgaagttgc agcaaagggg
      241 ccaggccctc gcatgtagtt ttttgacccg accgggtcgt ttatgcggcc cgtgtttgac
      301 tcactgtggt cagggagctg gtttgctgac tgcgcaactg ctctcaggtg aaccgaaagg
      361 aactgggtgc cctcggcgag gccgctgcgg tgcgtttgtt ggtccggttg ggatacaaga
      421 tccgtcacag gaactttcga tgtccgctgg gagagctgga tctcgtggct gaggagcgag
      481 gagtcctggc tttcatagag gtgaagacgc gcacgactgc ggagtttggc gtgccgctcg
      541 aggccgtctc tccggcgaag cagcgccggt tagtgcggct ggcgacctac tacctcgccg
      601 gccagaggat gcttgagcgg ccctgccggt tcgatgccgt gtccgtggtg atatccccgg
      661 gcggacgcat cgaggccgtc gagctcatcc gggacgcctt cgtccttcct gaatgactca
      721 cgtgagtcag tgtgcgatca tcaccgagcc tctacaatcc ctccggaggt catcacatgc
      781 tggccaaagt gcgcaccgcc gcggtgcaag gactggacgc gtatccggtc gatgtcgagg
      841 tcgacgtcgg gggtgggctt cccgcgttta ccatcgtcgg gttgcccgat acggcggtgc
      901 aggaggcaaa ggagcgtgtc cgcgcggcga tccggaactc gaactatgaa gttccctcgc
      961 gcaagatcac gatcaacctt gcgccggccg atgtccgcaa ggaaggtccg gcgtttgacc
     1021 tgccaatggc gatcggcatt ctcctcgcca ctgagcagat ccatgcatcc gggctcgatg
     1081 gggcgatggc gctgggggag ctctcactgg acggcggcat ccgtcctgtg ccgggcctcc
     1141 tgtcgatcgc gctggcggcg cgcgcgggtg gaatgcacac gctgattgcg ccggcggtca
     1201 acgcgcagga ggcagcactc gtcgagggcc tgcaggtgta cgcagtgcgc tcgctgttcc
     1261 aggtcatccg tcacctggaa ggagaggaag tgctgacgcc ggtgcgcagc ccgccgcaca
     1321 gcggcgccgg cgccgactat ccggtggatt tcgcagaagt gaaaggccag gcgtacgcgc
     1381 ggcgagctct ggaaatcgcc gcggcgggtg gccacaatgt attgatggtc ggcccgcccg
     1441 gggccggcaa gacgatgctt gccaggcggc tgccgacgat cctcccgccg ctgatctggg
     1501 atgaagcgat cgacgtgacg cgcatttaca gtgtcgccgg cctactgaac gggcgcggcc
     1561 ccgtgcacga gcggcctttt cgcagcccgc atcacagtac gagcagcgct gcgctcgtgg
     1621 gcggcggaac ggtgccgcgg ccgggagagg tgacgctcgc gcatcacggc gtgctcttcc
     1681 tggacgagct tccggagttt caccgcaacg cgcttgaagt gttgcgccaa ccgctcgagg
     1741 aagggttcgt gaccgtggcg cgtgtgcagt cgaccgtcac cttccctgcg cgcttcatgc
     1801 tggtggcggc aatgaatccc tgtccctgcg gctatcacgg cgatggtttg cgcgaatgcc
     1861 tgtgcacgcc atcgcagatc gcgcgctacc tcgggcgcgt gtcgggcccg ctgctggacc
     1921 ggttcgatct gcatatcgag gttccgcgcg tgccgagcgg agatcttgtg accacgctgt
     1981 ccgcggagcc ttcacatgag atccggacac gggttttgca cgcgcgggcg cgccagcaga
     2041 aacggttgga cagtccgagc ggctgcaatg ccgggatgac cggccggtcg ctccgcaagt
     2101 attgccggct cgatgaagac ggacggacgt ttctacgtgc ggcgatcgaa cggctgggac
     2161 tcagcgcgcg tgcgcacgac cgcatcctcc ggctcgcgcg cacgattgcg gatctggaag
     2221 ctgtcgatgc actcacgact gcgcacatcg cggaagccgt acagtaccgg gcgtttgatc
     2281 gaccgctgcg gctgctcgct tgacaccctg tgctatccta atgggcgaca atgacggcat
     2341 ttacgttgtc gcgcgtgatg atggggaata tccggcagct ttgcggccga tccgcgatgc
     2401 cccacgcgag ttattcgtgc gcggtacact gctgccacaa gatgagatcg cggtcgcgat
     2461 cgtgggcgcg cgccgtgcga gcgagtatgg caaagcggtg gcgcggcggc tcgccgcgga
     2521 tctcgctgcg tgcggcgtca ccatcgttag cggcatggcg cgggggatcg acggcgccgc
     2581 gcatcagggc gcgctcgatg ccggcggccg gacgctggcg gtgctcggct gcggcccgga
     2641 cgttgtctat ccaacagtgc acgcgcaact gatgcgacgg attatctgct cgggtgccgt
     2701 catctctgaa ttccggccgg agacgccgcc gcgtcccgaa cagtttccgc agcgcaaccg
     2761 tatcatcagc gggctggcga aaggggtcgt ggtggtcgaa gggcgggttg ggagcggatc
     2821 attgatcacg gcggatttcg cgctcgagca gggtcgtgag gtgtttgccg tgcctgggaa
     2881 catctttgag gagacgagct gggcgccaca ccacctggta tcgcagggcg ctcgactggt
     2941 ggcatcggcc gatgacattt tggacgagct caagatttcg catcgatccg cgccgcaacg
     3001 tgcggctgtc tcgctggacg atggagagcg tgccgtgtgg gcgcaactct cgctcgtccc
     3061 gcagcacgtc gatcggatcg cgatattgtc cggccaaccg gtcgcgctga ccagtcgcat
     3121 cttgctctct ttggaagcta agggtctggt gggcgcgctc gccgggcagc ggtatgtgaa
     3181 gaaggtgtaa ctgacaatgc caaaacgcaa agtgtcgacg gacggcgctg cgccaaagaa
     3241 gcggatgaaa gcaccgcttc ccgaggtgcc ggcggcgccc gaaccacaac tgggtgaatc
     3301 tgccaacggc gagagtcggg ccgcagcggg caagccgcgc tctcttgtcg tcgtggagtc
     3361 gccgacaaaa gcacgcacgc tgaagaagct cctcacccgc aggtacgacg tcttcgcttc
     3421 catggggcac ctcaaggatc tgccgcgcag ccagctcggc gtggacgtca agaacggctt
     3481 cacgcccaag tacatcgtgg tcaagggcaa gggatcgatc ctcaaggaac tcaaggaagc
     3541 cgccaagcag gcacccgtgg tcttcatggc gaccgaccct gaccgcgaag gcgaggcgat
     3601 ctcctggcat ctcgccgatg cgctgcgttc cgtgaacccg gagatcaagc gcatcgagtt
     3661 ccatgaagtt acgaaagacg ccgtccgccg ggcccttgcg aatccgcgcg acatcgatct
     3721 caatctcgtc aatgcccagc aggcccgccg catcctggac cggctggtgg gctacaagct
     3781 gagcccgctc ttgtggcgca agatccgcgg gggattgtcg gccgggcgcg tgcagtcggt
     3841 cgcagtccgg ctcgtcgtcg agcgggagcg tgaaatcgaa tcgttcgtgc cgcaggagtt
     3901 ctggagcctg ctggcccggc ttcgcaaaga cgaccagcag atcgaggcac gtctgtacgg
     3961 caagaacggc acgcggtacg gttcgccggg cgaagaggac gtcacagtgc ttcgcacgag
     4021 tgactccgtc gaggcgatcg tccgcgatct gggcggtgcc acatacacag tcggtgaggt
     4081 tcgccggaag gatcagttcc gcaaccctgc gccgccgttc accacgagca cgctgcagca
     4141 ggaagccaac cgcaagctcg gctactccgc tgcacgaacg atgaacgtgg cccagcaact
     4201 gtatgaaggt gttgatctcg gccccgaggg gaccgtcggc ttgatcacct acatgcgcac
     4261 cgattcggtg cgcgtctcgg agggtgccca gcgcgaggcg cgagagtaca tcacgcagac
     4321 gtatggagat tcctacgtgc cggacgtccc gcgcgtatac cgatcgcggc gagaagcaca
     4381 agaggcccac gaggccatcc gcccgacggc cgtgcggcgg gctccggacc gagtgaagcc
     4441 gttcctccgc agcgaccagt acaaagtcta caaactcatc tgggaacgat tcctcgcgag
     4501 ccagatggcg tccgcgatta tggacacact gtccgttgac attcatgccg gcagctatct
     4561 gttccgcgcg accgggtctc gcgtgaagtt ccctggattc ctcgcggtgt atcgtgaggg
     4621 cacagacgca aacggcgaag agaaagaggg ctggctgccg gagctggcga ccggggacag
     4681 gctcacgctc atggaactgg caccgcacca gcacttcacc caaccgccgc cgcgcttcac
     4741 ggaagcatcg ctggtgaagg cgttggaaga acgcggcatc ggacggccca gcacgtatgc
     4801 gccgaccatt gagaccatca aacggcgtgg gtacgtacag tccaccagcc ggcggctgca
     4861 gccgaccgac ctgggcgttc tggtcaacga catgctcgtc gagcacttcg gcgatgtcat
     4921 cgactatgac ttcaccgcaa cgatggaaga ggagctcgac caggtcgaag aggggcggct
     4981 ggactggaag aaagtcgtcg gcgacttcta caagccattc gagcacgacc tcgtcatcgc
     5041 agaaaagaaa atcgaagaaa tcaagatgcc tgtggtcgag atcggcgagg cgtgccctga
     5101 atgcgggcgg ccgctggtgc gcaagcacgg gcggttcggc gagttcatcg cgtgctcggg
     5161 gtttcccgag tgtaaataca cacgcccgct ggggatcggt gtgccgtgcc cgaaatgtaa
     5221 ggttggcgag gtggtggagc gccgcagcag gcgcgggcgg acgttctttg ggtgcagcac
     5281 gtatcccgcc tgtacgttca cgagctggga caaacccacg gagaaggtgt gcccccgctg
     5341 cggcaagaac gtgctcggca ttcaccagac cgcgaggaag atgacattgc gatgtctgga
     5401 tcgcacctgc ggctacaccg aaccggtgac gccggagccg gcagaggagc cggttccccg
     5461 ctaagagaag cctcgctgcg tgcaacgcga catcgacgga ttcctggccg cgcttgaagg
     5521 cgagcgcggc gtttcttcgc atacgcttac cggatacggt cgagacctcc accacttcgc
     5581 gcgctttctg gccgctgagc gcgtcacggc atggtccgcc gtcactcccc agattgcgag
     5641 gcggtacgtg gccagcatgg accggcgcta cgcgcgttcg tccgtggcgc ggcggttgtc
     5701 cgcgctgcgg acgttctaca aattcttata tcgtgagggc gccgtcagta agaacccgct
     5761 cgtcctggta ggcacgccca agcagccccg ccggctcccc aagtttctca cgccccacga
     5821 agttgccgcg ctgctcgccg caccaaacct ggcgacgccg atcggcctgc gggacagggc
     5881 gctcctggag gtcctgtacg cggccggcct tcgcgtcggc gaattgacgt cactgcgatc
     5941 cggcgacctt ccctcgggcg atgagatcag gatcgtgggc aaaggccgca gagaacgtat
     6001 cgccctgctc acacgctctg ccctggaggc gcttcgtgcc tacgtcgagg aaggacgccc
     6061 ttccctggca cgacgccgcg acatcgacgc cgtgttcctc aacgcgcgag gcggcgccct
     6121 ctccgaccgg ggtgtgcgcc tgatcgttga ccggcacatc cgcaaggtcg catctgaccg
     6181 gcggatcagc ccgcacgcac tgcggcacac ctttgcgacc cacctgctcg atgggggcgc
     6241 cgatctccga tctgtgcaag aactcctggg ccaccggagc ctcgccacga cgcagatcta
     6301 tacgcacgta tcgcgtgact ggctcaagcg cgtctacgac agggcgcatc ccagagcgtg
     6361 acttttgtgt tatactgcct tgccacatat tacgcacgct ctctgagccc accggtggtg
     6421 cctcagtagg caccctgagc gacgtcgaag ggtgccgcgg ggttccggtc gtcgggaatg
     6481 aggagggcgg agggaacaac caaagggagg taacgcattg cctgtcgtca cgatgaagca
     6541 actcctcgaa gccggggttc acttcggcca tcagacccgc cggtggaacc ccaagatggc
     6601 ccgcttcatt ttcaccgagc ggaacggcat tcacatcatc gatcttcaga agagcgtgcc
     6661 catgatcgag gaggcgtacg ccaccgtgcg tgacgccgtc gcgaacggag gaacctgcct
     6721 gttcgtcggt accaagaaac aagcgcagga cgcgatccgc gaggaatcca cccgcgcgtc
     6781 gcagccgtac gtgtgccagc ggtggctcgg cggaatgctc acaaacttcc agaccatccg
     6841 caagcgggtc gaacggctgc gcgagatcga ggatatgcgc gatcgagggc tgatggacgt
     6901 tctccccaag cgcgagcaag cgaggctgct cgaagaactg gcgcgtctag agaaatatct
     6961 cggcggtatc aaggcgatgt cccagctgcc atctgcagtc tatgtcgttg acacacgcaa
     7021 agaacacatc gccatcgccg aagcccgaaa actgggcatt cccgttgtcg ccatcatcga
     7081 tacgaattgc gatcccgatg aggtcgacta tccgattccg ggcaatgatg acgccatccg
     7141 ggcggtgcgc ctgatcacga gccggattgc caacgccgcc atggaaggac tcgaagagcg
     7201 gcggaagcgt gaggtcgtgg aggagaccgg cgaacccctc gcggcgtcag aggcgcccga
     7261 ggccgaatcg tttgacgaga aagctgtccc cgcggaggcc ggaatcgaag cgttgcctga
     7321 gccggttccc acggagacat tggtcgagaa cgaggtgtcg tagtggatgc aaagcggacc
     7381 gctactgcga ctagtcagac agagct
//
LOCUS       DBNGSB010000139         3372 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_1939815, whole genome shotgun sequence.
ACCESSION   DBNGSB010000139 DBNGSB010000000
VERSION     DBNGSB010000139.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_1939815"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /locus_tag="AC159B_03870"
     CDS             <1..1134
                     /locus_tag="AC159B_03870"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3376920.1"
                     /translation="AVNPSLEHAARNLGADEWTVVRTISLALARPGIAGAMLVVAISV
                     LADFGNAVIIAGGFPLLATEAWFRMEGMGDLKGAALVVAMLLVPTVLLFFLERFWVSR
                     LVYTAITGRGSRIARPPTPPVLKWGSVGVCTFAGVMILLVYVGVIAGAFTTVWGRDWS
                     LTLDHWRLAADRIASLWASVKIGVLAGLITGAVGVLVAFLTSRRLPLRQVLDFMAVLP
                     GALPGVFVGVGYVLAFNATPLGGTPWVLALALAFWHLPMGYQAASARLKQIERAIEEA
                     AVNLGANGLRVLRDIYFPLLLRALIEAFAVSFIRAVTNVSIVIFLITPGQVVATFVIL
                     NMIQNNIWGAAAALTTMLLLLTFASIGITWSTVARVVRTPGIA"
     gene            1131..2255
                     /locus_tag="AC159B_03875"
     CDS             1131..2255
                     /locus_tag="AC159B_03875"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3376921.1"
                     /translation="MTTEQPPNHSLRSGSAPAPNHSLRSGSGPAHVRLRNVTKRFGSA
                     IAVNDVSFDVAQGSFTTLLGPSGCGKTTILRMVAGFYRPDAGDIFLRDVKVTDIPPHR
                     RNTAMVFQEYALFPHMTVAENVGYGLRMRRVSPPEAGPRIAQVLELVGLSGHELKFPH
                     QLSGGQQQRVALARALVVEPEVLLLDEPLSNLDAKLRVRVRTEIRQLQQQLRKTALYV
                     THDQEEALAISDRIAVMDRGRILQSGTPLEIYHRPANRFVADFVGLANFVDARVVAAG
                     RVEARGMTLAITDGAPSGPVTLLLRPEGMRLHRRPPADAANVLGGRITAASFLGTLAR
                     YWVDVNGMHWTIDVPSPGDQLLSGEVFVEIPPDRIHILKE"
     gene            2271..3236
                     /gene="iolN"
                     /locus_tag="AC159B_03880"
     CDS             2271..3236
                     /gene="iolN"
                     /locus_tag="AC159B_03880"
                     /EC_number="3.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-scyllo-inosose hydrolase"
                     /protein_id="HIR3376922.1"
                     /translation="MAKWSLPPKGGHMDRRTGIYLQTMTMRDIEERQKVNDVLIVPLG
                     ATEAHGPNAPIGEDIFLVTRMAEAVAERTGCTVSEPLWFGSHPYHHIGMPTTVPIPED
                     TFNEFLRAMIAGFWNMGFRKQILLNGHGQEYVIPTAIHQFAKKYRVPALIINLNWYHA
                     VPTHFKLKSEGGPYETPFIHADEAETSWCLALFPELMKQEWAVDNNPFGFLPEGHVDK
                     AGNLLRRPINWYGHVGAGPIEIKAYIEGVVGKPSLAKAEKALPGVEALLTYLEKLVTD
                     IMETFPPGKLPPAEYVTERTKEELEPILKGPAKGGKSIYSLGWPP"
BASE COUNT          612 a         1027 c         1060 g          673 t
ORIGIN      
        1 gctgtgaacc ccagcctcga acacgccgcg cgcaacctgg gcgccgacga atggactgtg
       61 gtgcggacga tctcattagc cctggccaga ccaggcatcg ccggggccat gctcgtcgtg
      121 gcgatctcgg tgctggcgga ttttggtaat gcggttatca ttgccggtgg gtttccgctg
      181 ttggccaccg aggcctggtt tcggatggaa gggatgggag acctgaaggg tgcggcgctg
      241 gtggttgcga tgctcctcgt gcccacggtg ctgctgttct tcctggaacg gttttgggtg
      301 agccgtctag tgtacaccgc gatcacgggt cgtggttccc gcatcgctcg acctccgacg
      361 ccgccagtgc tgaagtgggg atccgttggt gtttgtacat ttgccggcgt gatgattctg
      421 ctcgtgtacg ttggggtcat cgcgggagct ttcacgacgg tctggggccg ggactggtcg
      481 ctcaccttag accactggcg cctcgcggcc gaccgcatcg cgtcattgtg ggcgagcgtc
      541 aagatcggcg tccttgcggg tttgatcacg ggagcagtcg gcgtcctggt cgcctttctc
      601 acctcccgtc gcctcccgct gcgccaggtg ctggacttca tggcggtcct gccgggtgca
      661 ctgcctgggg tgttcgtcgg tgtagggtac gtccttgcct ttaatgcgac tccccttggg
      721 ggaacgccat gggtcctcgc gcttgcgctc gccttctggc acctgccgat gggatatcaa
      781 gcagccagtg cccggctgaa gcaaattgaa cgcgccattg aggaggcggc ggtcaacctc
      841 ggtgcgaacg gcctgcgggt gctgcgtgac atctactttc cactcctcct gcgagcgctc
      901 atcgaagcct ttgcggtctc gtttatccgt gcggtgacga acgtcagcat cgtgattttt
      961 ctcatcaccc ccggacaagt cgtcgccacc ttcgtcatcc tcaacatgat ccagaacaac
     1021 atctggggcg cggcagcggc gctcaccacg atgctcttgc tcctcacgtt tgcgtcgatt
     1081 ggcatcacgt ggtcgacagt agcgcgggtg gtgcgcacac ccgggatcgc atgacgaccg
     1141 agcagccccc gaaccactcg ctgcgctcag gttcagcgcc agccccgaac cattcgctgc
     1201 gctcaggttc agggccagcc cacgtccggc ttcgcaacgt caccaaacgg tttggttccg
     1261 cgatcgcggt caacgacgtg tccttcgatg tggcgcaagg cagcttcacg accctgctcg
     1321 gtccttccgg gtgcgggaag acgaccatcc tgcggatggt ggcgggattc taccggcccg
     1381 atgcggggga catcttcttg cgcgacgtca aggttacgga tattccccca catcggcgaa
     1441 acacggccat ggtgttccag gagtatgcgc tgttcccgca catgaccgtg gcggaaaatg
     1501 tgggctacgg attgcgcatg cgccgggtca gtccccctga agccggtccg cgcatcgcgc
     1561 aggtgctcga actagtggga ctctccggac acgagctaaa gtttccccac caactttccg
     1621 gcggccagca gcagcgggtc gcgctggcgc gggcgcttgt cgttgaaccc gaggtgcttc
     1681 tcctggacga accactctcc aacctggacg cgaagttgag ggtccgagtc cgcacggaga
     1741 ttcggcagct gcagcagcag ttgcgaaaga cggccctgta cgtcacgcac gatcaggagg
     1801 aagcgctggc gatctcagac cggattgcgg tcatggaccg cggtcgcatc cttcagtccg
     1861 gtacgccgct ggaaatttat catcgacccg ccaatcggtt cgtcgccgat ttcgtcggtc
     1921 tcgccaattt cgtcgatgcc cgggtcgtcg ctgcgggccg cgtcgaagcc cgcggcatga
     1981 cgctggctat cacagacggc gcaccgagtg gaccggtcac gctgctcctg cgtcccgagg
     2041 ggatgcggct gcaccgccgg ccgccggcgg acgcggcgaa tgtgctgggc gggcggatta
     2101 cggcggcgag ctttcttggt acccttgccc gatactgggt cgatgtgaat ggcatgcact
     2161 ggaccatcga cgtgccgtct cccggcgacc agttgctgag cggcgaggtt tttgtcgaga
     2221 tcccgcccga tcggattcac attttgaaag agtaaccaga ggaggccgaa atggcaaaat
     2281 ggagtctccc gccgaagggc ggacacatgg atcggcgcac tggaatctat ttgcagacca
     2341 tgacgatgcg ggacatcgaa gagcgtcaga aggtcaacga cgtactcatt gttccgctcg
     2401 gggcgaccga ggcccatggc ccgaacgcgc cgatcggcga ggacatcttt ctcgtcaccc
     2461 gcatggccga ggcggtcgcc gaacgcacgg ggtgtacagt atcagaacct ttgtggttcg
     2521 gatcgcaccc gtaccatcac atcgggatgc cgacgaccgt gccgatcccc gaggatacct
     2581 tcaatgagtt tctgcgcgca atgatcgcgg gattctggaa catggggttc cgcaagcaaa
     2641 tccttctcaa cgggcacggc caggagtacg tgataccgac cgcgattcac cagtttgcga
     2701 agaagtaccg ggtgccggcc ctcattatca acctcaactg gtatcatgcc gtgcccacgc
     2761 acttcaaact gaagtcggag ggcggtccct acgagacacc cttcatccac gcggacgagg
     2821 cggaaacgtc atggtgtttg gcattgttcc cagaactgat gaagcaggaa tgggcggtgg
     2881 acaataaccc gttcggcttt ctgcctgaag ggcacgtgga caaggccggt aacttgctgc
     2941 ggcggccgat caactggtac ggccacgttg gtgcgggtcc gatcgagatc aaggcgtaca
     3001 tcgagggtgt cgtcggcaag ccctcgctcg ccaaagctga aaaggcgctc cccggcgtag
     3061 aagcgcttct cacctatctc gagaaactcg tgaccgacat catggagacc ttcccacctg
     3121 ggaaactccc gccggcagaa tatgtcaccg agcgcaccaa agaggaactc gagccgatcc
     3181 tcaaaggacc cgcgaagggc ggaaagagca tttattcttt ggggtggccg ccgtgacaca
     3241 caaagacggt cgtaggtcgt gggtcatagg tcgtggtaag gaccaggcag cctacgaccc
     3301 acgacccacg acccacgacc gcagttccgg atgaagctcg ccttggttgt ctcgatcgaa
     3361 gagacaggat tt
//
LOCUS       DBNGSB010000140         5470 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_561292, whole genome shotgun sequence.
ACCESSION   DBNGSB010000140 DBNGSB010000000
VERSION     DBNGSB010000140.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 5470)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5470)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5470
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_561292"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..825)
                     /locus_tag="AC159B_03885"
     CDS             complement(25..825)
                     /locus_tag="AC159B_03885"
                     /EC_number="3.5.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010042141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016403 - dimethylargininase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylarginine dimethylaminohydrolase family
                     protein"
                     /protein_id="HIR3376923.1"
                     /translation="MTRLLVCAPDYFSIDYEINPWMRLSNAADPERARAQWHGLMRTL
                     EHEIGVLLERMPPVPGLPDMVFTANAGSVMGRRAVPSRFRHPERQREERHFEEWFREH
                     QYEVILLDEGLYFEGAGDLLGFQDVWFGGYRQRSDIRAYARLSEIFGREIIPLELVDP
                     RFYHLDTCFCPLSGGELLYYPPAFDPYAQRVIKDRIDTARRLVVPEPDALRFACNAVC
                     VGKHIIVPEGCSDTMKLLAGHGYEPHPLPLDEFMKAGGSAKCLTLALD"
     gene            complement(822..2051)
                     /locus_tag="AC159B_03890"
     CDS             complement(822..2051)
                     /locus_tag="AC159B_03890"
                     /EC_number="4.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008473 - ornithine cyclodeaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase, nickel-pincer
                     nucleotide-dependent"
                     /protein_id="HIR3376924.1"
                     /translation="MASLQETILLHGHIIDSLILAKVLDTIHMMGGTFDLNEVHIGAT
                     REDPSHAKIVVRAPSASLLAEILEAIQPHGATVERESDCAVEPAPADGILPDTFYPTT
                     HLPTQVRLGGRWIDVDAIEMDLAIRTDSGFSSARAVPMCAAKKGDLIVTGRNGIRVIP
                     LQRPRERDVFAFMEARVSSERPHAPLIADIAHRMQALHDQGVGKVLLAGGPAIIHSGG
                     REALAWLIESGFIHVLFCGNALAAHDMEAHLFGTTLGFGLSAGRAVPHGHEHHLRTIN
                     RIRAIGSIQRAVETGVITQGIMAACIRHRVHLVMAGTIRDDGPLPGVITDSIAAQEAM
                     RAALPGVALALLVASTLHSVATGNLLPATVPTVCVDVNPAVPTKLADRGSFQAVGLVM
                     DAASFLRELARELGWKA"
     gene            complement(2253..2597)
                     /locus_tag="AC159B_03895"
     CDS             complement(2253..2597)
                     /locus_tag="AC159B_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376925.1"
                     /translation="MKTLLSAVAGLGVVFSLSLALANPALLPKHPGYPASGNANDTGQ
                     TNMAGEKAILEGAKADSKHTMQELKPEDGRTAIINEEGAGRLPKHPGYYNYKIEPPVN
                     SATKIGADRVIQ"
     gene            complement(2744..3721)
                     /locus_tag="AC159B_03900"
     CDS             complement(2744..3721)
                     /locus_tag="AC159B_03900"
                     /inference="COORDINATES: protein motif:HMM:NF024954.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HIR3376926.1"
                     /translation="MQVFGFPGALLRSRHLPLPDRSRAAHTRWQALAVWQKTGQWQAA
                     RDIFNVSRATLYRWRQQWETSGTLEARSRRPHRVRQPQTPPPVVRRLRELRTQYPRWG
                     KAKLVVLLRREGVHLSASTVGRLLHRLKRSGQLVESRPLASRVARHRPRPWAQRLRHV
                     PPARQPGDVIPVDTLQLRPLPGIRFYQCTARDVVSRWDVLEAFTTASATTATLFLTTL
                     QRRCPFAIRALQVDGGSEFKAAFEAACHAHQLPLSVLPPRSPKLNGHVERANGTHRHE
                     FYEVLEDCPWTVAALKARLRRWEHLYNHVRPHQALAYLTPAEFLTMTLP"
     gene            3903..4937
                     /gene="tdh"
                     /locus_tag="AC159B_03905"
     CDS             3903..4937
                     /gene="tdh"
                     /locus_tag="AC159B_03905"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - L-threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HIR3376927.1"
                     /translation="MKALVKTAPTPGLTLQPMPDPRPGEAEVLIRVKATSLCGTDAHI
                     YQWDEWAQGRIHPPRIIGHEMCGEVAELGQGVTTMAVGDYVAAESHLTCGVCFQCRTG
                     QAHVCKNYRILGVDVDGSYAEYVVLPERVLWKTSREIPPELACAQEPLGNAVYATLIE
                     DVTGKSVLISGCGPTGLFAAAIARVAGASPIIATDVSDYRIGLAKQLGVDHVLNAKSE
                     SPEAIATAIRDITGGEGVDAALEMSGYPGALHQAFRSVKNGGRVSLFGIPTGQVSFDL
                     PNEIIFKGIRVYGVTGRRLFDTWYRLAGLFKAGLDIRPVVTHTFPMADFAKGFDLIKA
                     GQCGKVVLFP"
     gene            5137..>5470
                     /locus_tag="AC159B_03910"
     CDS             5137..>5470
                     /locus_tag="AC159B_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006084744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR3376928.1"
                     /translation="MAFQSFKKVVEQQLADIRAAGLYKTERQILVPQGAEIRVAKGEV
                     LNLCANNYLGLANHHDVVQAAVEGLRRHGYGMASVRFICGTQDLHKRLEQDLATFLGT
                     GDTILYSSC"
BASE COUNT         1121 a         1569 c         1746 g         1034 t
ORIGIN      
        1 tagctatgag ccgtttgcaa ttagctaatc caaggccagc gtcaaacact tcgccgatcc
       61 gcccgccttc atgaattcgt cgagcggcag cggatgcggc tcgtacccgt gtccggccaa
      121 gagcttcatc gtgtccgaac agccttccgg cacgataatg tgcttgccga cacagaccgc
      181 gttgcaggcg aagcgaagcg catcaggctc aggcacaacc agtcgtcgcg cagtgtcgat
      241 gcgatccttg atcaccctct gcgcgtaggg atcgaaagcc ggggggtaat acagcaattc
      301 tcctccactg agcgggcaga agcaggtatc caaatggtaa aagcgggggt ccaccagttc
      361 gagggggatg atctcacgcc cgaagatctc actcagccgg gcataggctc ggatatcgct
      421 tcgctgacga tagcctccga accagacatc ctgaaatccc agaagatcac cggcgccttc
      481 aaagtagagg ccttcgtcga gcaggataac ctcatactga tgctccctga accactcctc
      541 gaaatgtcgt tcctcacgtt gccgttccgg atgccggaag cggctcggca ccgcccgacg
      601 acccatcacg gatcccgcat tagccgtaaa taccatgtcg gggagtcccg gaaccggcgg
      661 catccgctcc aacagcacgc caatctcgtg ctccagcgtt cgcatcagtc catgccattg
      721 cgcgcgggcc cgttcgggat cggcggcatt cgagaggcgc atccaggggt tgatctcata
      781 atcgatgctg aagtagtccg gcgcgcagac caataagcga gtcatgcttt ccaccccagc
      841 tcgcgtgcca gctcgcgcaa gaatgaggcg gcgtccataa caaggcccac ggcctgaaag
      901 ctgccgcggt cggcaagctt cgtcgggaca gcgggattga cgtccacgca taccgtgggg
      961 acggtcgccg gcaggagatt tccggtggcg acggaatgga gagttgacgc aacgagaagc
     1021 gccagagcga cccccggcaa cgcggctcgc atcgcctctt gcgcggcgat ggagtcggtg
     1081 atcacgccgg gcagagggcc atcgtcgcgg atcgttcccg ccatgaccag gtgcacgcga
     1141 tggcggatac aggccgccat aatcccctgc gtgatcacac cggtctcgac cgcgcgttga
     1201 atgcttccaa tggcgcggat gcgattaatc gtccgcaggt gatgctcgtg gccgtgtgga
     1261 accgcgcgac cggccgacag gccgaatccc agtgtcgtgc cgaacagatg ggcctccatg
     1321 tcgtgagcgg ccaaggcgtt cccgcagaag aggacgtgga taaacccgga ctctatgagc
     1381 caggcaaggg cttcccggcc gcccgaatga ataatggcgg ggccgcccgc cagtaagacc
     1441 ttgccgacac cctggtcatg aagcgcttgc atccggtgcg cgatgtcggc gatgaggggg
     1501 gcgtgagggc gctcagagga cacgcgcgct tccatgaagg cgaacacgtc gcgttcgcgg
     1561 ggtcgttgca ggggtattac gcggatgccg ttccggccgg tgacgatgag atcgcccttc
     1621 ttcgcggcgc acatgggaac cgctctggcg gaggagaagc cggaatctgt ccggatcgcc
     1681 agatccatct caatggcgtc cacgtcaatc cagcggcctc caaggcggac ctgagtcggc
     1741 aagtgcgtgg tggggtagaa cgtgtccggc agaattccat cagccggcgc cggctccacc
     1801 gcgcagtccg attcccgctc aaccgttgcg ccgtgcggtt gaatggcctc aaggatttca
     1861 gccaagagcg aggcggacgg cgcacgcacc acaatcttgg cgtgagatgg gtcttcgcgg
     1921 gtggcgccga tgtgcacctc gttgagatcg aacgtgccgc ccatcatgtg aatggtatcc
     1981 agtaccttcg ccaggatcag tgaatcgatg atatgtccgt gaagaagaat ggtttcttga
     2041 agggaggcca tggcaacctc aggcggtgta tcgctcaggg atctatcgat atgataccac
     2101 gctgccgatt tcatctgtcg tggacctgtc gaggggcatt gaagtcaccg aagggaaagt
     2161 cggttggaag aaatgaacag agggaagagg gcgaacaaaa aggggagacc gcagagggcg
     2221 gtctcccctg tgtttgcttc aggcacggat tcttactgaa tgacccgatc ggccccaatc
     2281 ttcgtcgcgg agttcaccgg cggctcaatc ttataattgt agtatccggg atgcttcggc
     2341 aagcgcccgg cgccctcttc attgatgatg gcagtgcggc cgtcctccgg cttcaattct
     2401 tgcatcgtat gcttgctgtc agccttcgcg ccttccagta tggctttctc cccggccatg
     2461 ttggtctgtc cggtatcatt cgcgttcccg gatgccgggt agccagggtg cttggggagc
     2521 aaggccggat ttgcaagcgc aagcgagagg gagaagacga cacccaatcc tgcaacagcg
     2581 ctaagaagtg ttttcatgac ccggactcct tttattgaag gaattaatat tggtgaaaga
     2641 gagcaaagag tttcgaacaa cagcccataa tgggggcatc ttaaacgcct cgtttgaccc
     2701 tgtcaagtac atgtcctggt tcactacatc tgagacggga gcctcacggc aaggtcatag
     2761 tgagaaattc ggcgggtgtc aaataggcga gcgcttggtg cgggcgcaca tggttgtaga
     2821 gatgttccca acggcgaaga cgggccttga gggcagcgac ggtccaggga caatcctcca
     2881 gcacctcgta gaattcgtgc cgatgggtgc cgttggcccg ttcgacgtgg ccattgagtt
     2941 tcggggagcg cggcgggagg acagagagcg gcaactggtg ggcatggcag gcagcttcga
     3001 aggcggcctt gaactcgctc cccccatcga cctgtaaggc gcggatggca aaggggcagc
     3061 ggcgctgcaa ggtggttaag aacagggtgg cggtggtcgc gctggcggtg gtgaacgctt
     3121 cgagcacatc ccagcggctg acgacatcac gggctgtaca ttggtagaac cggatgccgg
     3181 ggagggggcg aagctggagg gtatcgactg gtataacatc gcccggctgg cgtgcgggcg
     3241 gcacgtgtcg caggcgctgc gcccagggcc gcgggcggtg acgggcgact cgtgaggcca
     3301 gggggcggga ttcgaccagt tggcccgaac gcttcaagcg atggaggagc cggcctacgg
     3361 tggaggcgga caagtggaca ccttcgcgcc gcagcaagac gacgagcttg gccttgcccc
     3421 atcggggata ctgcgtgcgc agttcgcgga ggcgccggac gactgggggc ggggtctgtg
     3481 gctgccggac ccgatgaggc cgccgggagc gggcctccag cgtgccggag gtctcccatt
     3541 gctgccgcca ccgatagagc gtggcgcggg agacattgaa aatgtcccgt gcggcttgcc
     3601 actggccggt cttctgccag acagcgaggg cttgccaccg ggtgtgggcg gcacgggacc
     3661 gatctggtaa cgggaggtga cgactccgga ggagcgcccc gggaaaaccg aacacctgca
     3721 tcgagagcct cctttcgagg ctcccgttct accagcaatc gtctcagatg tatctgaacc
     3781 tagacagagc gtggtgcctt cgaagtgact atgccataat gaactcaggc aaccttgtca
     3841 cactgtcttg aacaaccggg gaagggtgcg atcgacgtgt ccgaaagagt gagaaccgcg
     3901 cgatgaaagc tcttgtcaaa acggcgccga cgccggggct cactttgcag ccgatgcctg
     3961 acccgaggcc cggcgaggcc gaggttctga tccgcgtgaa agccacctca ctctgcggca
     4021 ccgacgcgca catttatcaa tgggatgagt gggcgcaagg tcgtatccat cctccacgga
     4081 tcatcggcca cgagatgtgc ggcgaagtcg cggaattagg acagggggtc acgaccatgg
     4141 cggtgggcga ttacgtagcc gccgaatcac atctgacgtg cggcgtctgc tttcagtgtc
     4201 gcacggggca ggctcacgtc tgcaagaatt accgcatcct cggcgttgac gtagacggat
     4261 cctacgccga atacgtggtc cttccggaac gggtcctgtg gaagacatcg cgggagatcc
     4321 ctcccgagtt ggcgtgcgcg caagaacccc tcggcaacgc ggtctatgcg accttgatcg
     4381 aagatgtgac cgggaagtcg gtgctgatca gcggctgcgg cccgaccggg ctcttcgccg
     4441 cggccatcgc gcgagtcgcc ggggcctcac cgatcatcgc gaccgacgtc agcgattatc
     4501 gtatcggatt ggccaagcaa ttgggcgtcg atcacgtctt gaatgccaag agcgagtccc
     4561 ccgaagccat cgccacggcc attcgagata tcacgggagg cgagggtgta gacgccgcgt
     4621 tggaaatgtc cggctatccc ggcgcgctcc atcaagcttt ccggtccgtc aagaacgggg
     4681 gacgggtgag tctattcggc atacccacag gacaagtcag tttcgatctg ccgaacgaaa
     4741 tcatcttcaa gggaatccgc gtctacgggg ttaccgggcg ccgcctgttt gatacttggt
     4801 accgactggc aggtctcttc aaagccggtc tcgatatcag gcccgtcgtc acccatacgt
     4861 ttcccatggc agactttgca aaaggcttcg acctcatcaa ggcaggccag tgcgggaagg
     4921 tggtgttgtt tccttaacag gatgctgaaa aggccgtcaa tttcgttctg ggtcgttcga
     4981 acgtctcaac gtattcgaaa gtacgcctcg gcgtccaaaa tcccggcggc tcggctcggg
     5041 caaggagcgc gtcttggcgc gcttgggttg gcgggtgtaa tgcggccttt tgagcatcct
     5101 attatgacag ccactaaatt ttttgagtaa cccgaaatgg cattccaatc atttaagaaa
     5161 gtagtcgaac agcagttagc tgacatccgc gccgctggcc tctataagac cgagcggcag
     5221 atcctcgtac cgcagggcgc cgagatccgt gttgcgaagg gcgaggtcct gaacctatgc
     5281 gccaataatt atttaggatt ggcgaaccat cacgatgtgg tccaggcggc tgtggaaggg
     5341 ctccggcgcc acgggtacgg catggcttcg gtacgattca tttgcggcac acaggacctc
     5401 cacaagcgac tggaacagga cttggccacg tttctcggaa ccggcgatac catcttgtac
     5461 agctcgtgtt
//
LOCUS       DBNGSB010000141         2934 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2094802, whole genome shotgun sequence.
ACCESSION   DBNGSB010000141 DBNGSB010000000
VERSION     DBNGSB010000141.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2094802"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="AC159B_03915"
     CDS             complement(<1..194)
                     /locus_tag="AC159B_03915"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3376929.1"
                     /translation="MPDDAVTDAPVSTSRLSAFSPLRHRNFRLLWTGLVISNTGTWMQ
                     FVALGYLVDRLTQSPLYLGFL"
     gene            351..770
                     /locus_tag="AC159B_03920"
     CDS             351..770
                     /locus_tag="AC159B_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017424502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HIR3376930.1"
                     /translation="MRSKSLFAVFARRASRATGHPAAFVTAVLIVLVWAITGPLFHFK
                     DTWQLVINTSTTIVTFLMVFLIQSTQNRDSIAMHLKLDELIRALEGAHLALLDLEELT
                     EKDLERIRHRYEELARDAREELRKGHPDTGRPRVNVD"
     gene            complement(780..1127)
                     /locus_tag="AC159B_03925"
     CDS             complement(780..1127)
                     /locus_tag="AC159B_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376931.1"
                     /translation="MPTRWWHFFLHGQSAFLLKLTLFFRRRQGYRVMVVTDVPYYLTE
                     GATVPDQPPPAPLALQLALTAALCALTGAAFAVSLVRAWPEIAIEVLGIAFVITLAAL
                     TFLLLVRAVVSGR"
     gene            complement(1176..2831)
                     /locus_tag="AC159B_03930"
     CDS             complement(1176..2831)
                     /locus_tag="AC159B_03930"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR3376932.1"
                     /translation="MALASFKRMLVGPPLETRQLIHQRITKLKGLAVFSSDALSSTAY
                     ATEEILLVLVAAGAAALQLALPVAVGISAVLAIVAFSYYQTIHAYPSGGGAYIVAHDN
                     LGIWPGLVAAAALLIDYVLTVSVSVAAGVAAVTSAVPSLFGLRVWLAVGAIFVVAVIN
                     LRGVRESATIFAVPTYVFIGSLGILIAVGLGRTVGQPPVPAQVEPAGALAPLTMFLIL
                     RAYASGSAAMTGVEAISNGIPAFRPPESRNAGVTLIWMAAILITLFMGITFLAHQLGI
                     VPRGHETVVSQVARAILGSGPLYFTVQAATALILILAANTSFADFPRLSSILARDGFL
                     PRQLSNLGDRLVFANGIIVLALLSAALVFIFRADTHSLIPLYAVGVFLSFTLSQSGMV
                     KRWWTRRGPGWTRNLIINGIGALTTAVVLAVVVTTKFLHGAWIVLVLVPVNVWIMYRI
                     YAHYREVREQLTLEGARVPETIRKHKVVVPVGGMHRGVLPALRYAKSLGGDVVAVTVD
                     VDPSRTQDVQEKWALWGMDVPLKILPSPYRSVLDPLLRYLNNL"
BASE COUNT          648 a          877 c          931 g          478 t
ORIGIN      
        1 aggaagccga ggtatagtgg cgactgggtg aggcggtcga cgaggtagcc tagagccacg
       61 aactgcatcc aggttcccgt attgctgatc accagcccgg tccacagcag cctgaagttc
      121 cggtggcgaa gcggtgaaaa tgcggagagt cgagatgtgg agactggtgc gtcggtgacg
      181 gcgtcgtctg gcacgtacac aatctacacg atctccacaa tcctcacaat ctgcacaatc
      241 ccagaggtgc cgcaggtcat tttgatccgg aggaacaaca aatccaaatt agtagctagt
      301 gtggatcggg cagattgcgt agattgtgcg gattcttagg gaggtgagtc gtgaggtcaa
      361 agagcctgtt cgcagttttc gcgcggaggg cttcgcgcgc gaccggacat ccagccgcgt
      421 ttgtaacggc agtgctaatc gtcctcgtct gggcgatcac aggtcctctg ttccacttca
      481 aggacacctg gcagctggtc atcaacacta gcactacgat cgtcacgttc ctgatggtct
      541 tcctcattca gagcacgcag aatcgcgaca gcattgccat gcatctgaag ctggatgagt
      601 tgatccgcgc actggaggga gcgcacctcg cattgctgga cctggaggaa ctgaccgaaa
      661 aagatctcga gcggatccgc caccgctacg aggagctggc ccgcgacgct cgcgaggaat
      721 tacgaaaggg gcatcccgac accggacgcc cgagggtcaa tgtcgattaa aatccaagcc
      781 tagcgtccgc tcacgacggc cctcacgagc agcaagaacg tgagcgcagc cagcgtgatc
      841 acgaaggcga tgccgagcac ttcgatcgca atctccggcc acgcgcgcac cagggacacg
      901 gcgaacgcag cgccggtcag cgcgcaaagt gcggctgtca acgcaagctg caaagccagg
      961 ggcgcaggcg gtggctgatc ggggacagtg gcgccttcgg tgagatagta cggaacgtcg
     1021 gtgacgacca tgacccgata cccctggcgc cggcggaaga agagggtgag cttcagcaga
     1081 aatgcggact ggccgtgcag gaagaaatgc caccatctcg tcggcacgaa ctcgggaagg
     1141 acaaccgtga tctgctggtc gaagccgatc tcccattaca gattgttcag gtagcgcagc
     1201 agcggatcga gcactgaacg gtatggtgaa ggcaggatct tcaagggcac gtccatgccc
     1261 cacagtgccc acttctcctg cacgtcctgc gtgcgagacg gatccacgtc caccgtcacc
     1321 gccaccacgt cgccgcccag cgatttcgcg tagcgcagcg ccggcagcac cccgcggtgc
     1381 atccctccga cggggaccac tactttatgc tttcggatgg tctccgggac gcgcgcgccc
     1441 tcgagcgtga gttgctcacg gacctcccgg tagtgagcgt aaatgcggta catgatccaa
     1501 acgttaaccg gaacgagcac caggacgatc caggcgccgt ggaggaattt ggtagtcacg
     1561 accactgcga gcaccacggc cgtggtcagc gcgccgatgc cgttgatgat caggttgcgg
     1621 gtccagccgg gcccacgccg cgtccaccac cgcttcacca ttccgctttg cgacagcgtg
     1681 aacgaaagga acaccccgac cgcgtacaga ggaatcagtg agtgcgtatc cgcacggaag
     1741 atgaacacga gcgccgccga gagaagcgcg agcacgatga tcccgttcgc gaacaccaga
     1801 cggtcgccga ggttactgag ttggcgtgga aggaaaccat cgcgtgcgag gatggatgaa
     1861 aggcgcggga agtcggcaaa actcgtgttg gctgcaagga tgagaatcag cgccgtcgcc
     1921 gcctgcaccg tgaaatataa cggcccactg ccgaggatgg cgcgggcgac ctgcgagacg
     1981 acagtttcat gtcctctcgg aacaatccca agctgatgcg ccaggaacgt gatccccatg
     2041 aacaacgtga tgaggatcgc cgccatccag atcaacgtga cgccggcgtt gcgggactcc
     2101 ggagggcgaa acgccgggat cccgttgctg atcgcctcca cgccggtcat cgcggcggag
     2161 ccgctcgcgt atgccctgag gatcaagaac atcgtcagcg gcgccagcgc gccggcgggt
     2221 tcgacctgtg ccgggactgg tggttggcca acggtacgcc ccagtccgac ggcaatcagg
     2281 atccccaagc ttccgataaa cacgtacgtg ggaacagcaa agatcgttgc cgactcgcgc
     2341 acgccgcgga ggttgatgac cgcaaccacg aaaatcgcgc ctaccgcgag ccagacacgc
     2401 agaccaaata gcgaaggaac ggccgacgtg acggccgcca cgccggccgc gacgcttacc
     2461 gacacggtga gcacgtagtc aatcagcagt gccgccgcgg caaccaggcc cggccagatt
     2521 ccgaggttat cgtgcgcaac gatgtaggcg ccgcccccag acggataggc gtggatcgtc
     2581 tgatagtatg agaatgcgac gatcgccagg actgccgaaa tgcctacggc gaccggtagc
     2641 gcaagttgga gggccgcggc gccggcggcc acgaggacga gcaagatctc ttctgtggca
     2701 tacgcggtgg atgacagcgc atcggacgag aagaccgcga gccctttgag cttggtgatg
     2761 cgctggtgaa tcaactggcg ggtctctaac ggcggaccca ccagcatacg cttgaaagag
     2821 gcgagggcca tctgttttcg gggcgctcag cgcgcgtgta aactatctca cgagcgccgg
     2881 gggccttgaa ggcttggcag gacccggggg cgtcggttcc tcttcctgaa tcgt
//
LOCUS       DBNGSB010000142         3306 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_867845, whole genome shotgun sequence.
ACCESSION   DBNGSB010000142 DBNGSB010000000
VERSION     DBNGSB010000142.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_867845"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..1183)
                     /locus_tag="AC159B_03935"
     CDS             complement(155..1183)
                     /locus_tag="AC159B_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HIR3376933.1"
                     /translation="MTETQEQTATRATRSRDTSAVSPQAPKLLDQVRERIRVKHYSLA
                     TEKTYCYWIRFFIRQQGMRHPRDMGAAEVEAFLSWLATAREVSASTQNQAMHAILFLY
                     KEVLGITLPWLDTITRAKPSKRIPVVLTRAEVTALLGKIDGTPGLVARLRYGTGMRLM
                     EGLRLRVKDIDFAGRSILIRAGKGDKDRVVMLPDALAAPLKALLTERRKWHDIDLARG
                     KADVELPHALERKYPNAGKEFGWQFVFATSTYNTCHRTGAVRRHHLHPKTIQRAVKQA
                     ANAAGIEKPAHPHLLRHSFATHLLESGADIRTVQELLGHSDVSTTMIYTHVVNRGGRG
                     TVSPLDRL"
     gene            complement(1176..1400)
                     /locus_tag="AC159B_03940"
     CDS             complement(1176..1400)
                     /locus_tag="AC159B_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376934.1"
                     /translation="MRQTPAARPAAAGASTSPVADEGSKSAVIEAEREQLIFMVAADA
                     QLEFYDRLTSFNLAAMSHNLAAINGDPHHD"
     gene            complement(1541..1957)
                     /locus_tag="AC159B_03945"
     CDS             complement(1541..1957)
                     /locus_tag="AC159B_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376935.1"
                     /translation="MRQLGANDNHGVMSMESIKRTVRVTIEKEIEVELTPAVFGGMTQ
                     DEYLAEFRKGLWHVDSIDDVVKYAARTAATGGAGGEYDGIGLLNHDFSTYPRVPDVKI
                     RVIDEEYEEEILTPNVELTGAARPYRAASSDRRERR"
     gene            complement(2003..2764)
                     /locus_tag="AC159B_03950"
     CDS             complement(2003..2764)
                     /locus_tag="AC159B_03950"
                     /inference="COORDINATES: protein motif:HMM:NF013658.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase
                     domain-containing protein"
                     /protein_id="HIR3376936.1"
                     /translation="MDYVIFASYGNDSIALIQWAHERGLKNVTVAYSDTGWAADWWPA
                     RVTKAEAWVQTLGFEPVRIASEGMEALVMRKKAWPRGGGGKYQFCTAALKEEPALAWL
                     DALDPEKEVVCMVGIRREESDNRTNFPEWVEESEKHGGRNLHAPLVRHTEVDRNALLA
                     KTPFEPLPHRSKECYPCVNARKKEIAALPDEKITTILTAETLAGINSNGNPRVMFSPK
                     RHKGAVGIMAVVKWARGDTEPEPELAGCDGGWCGA"
     gene            complement(2794..2988)
                     /locus_tag="AC159B_03955"
     CDS             complement(2794..2988)
                     /locus_tag="AC159B_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376937.1"
                     /translation="MTTVYLAMKHQQHPYARGHHLEAGTVEEAFESKKAAAEFVAEKN
                     KRATYNLWSVKAKKVKPSNE"
     gene            complement(3091..>3306)
                     /locus_tag="AC159B_03960"
     CDS             complement(3091..>3306)
                     /locus_tag="AC159B_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376938.1"
                     /translation="FTEAVAVSIERQLRMRWGGARIEYIAKTTIEEAKAKDEALREAR
                     RTGRGREAGEQFGVSRATMYRMLKRSR"
BASE COUNT          512 a         1091 c          950 g          753 t
ORIGIN      
        1 cacaatgcaa gctccagttg ttgcggttcc gggtagtgcc aggcgttgcc gctgagcagc
       61 gcgcgccgga tggctttctc tgccttgatc tgggtagcgg tcggccactg gcgccggtgc
      121 gcggccaggt gtttggcgat ctgatagtcg aggttcatag ccggtcgagc ggggacaccg
      181 taccgcggcc gccgcggttg actacgtgcg tgtagatcat cgttgtgctc acgtcagagt
      241 ggccgagcag ttcctgcacg gtgcggatgt cggcgccgct ctccagtagg tgagtggcga
      301 aggagtggcg cagcagatgc gggtgcgccg gtttctcgat gccggccgcg ttggctgcct
      361 gcttgacggc gcgctgaatg gtcttcgggt gcaggtgatg tcggcgaacc gcgcccgtcc
      421 ggtggcaggt gttgtaggtc gatgtcgcga aaacaaactg ccagccgaat tccttcccgg
      481 cgttcggata tttccgctcc agcgcgtgcg gcagctcgac gtcggctttg ccgcgcgcca
      541 gatcgatgtc gtgccacttg cggcgctcgg tcagcaacgc cttgagcggc gctgccagcg
      601 catcgggcag catgacgact ctatccttgt cacccttgcc cgcgcggatc aggatcgatc
      661 ggccggcaaa atcgatgtct ttgacgcgca ggcgcagccc ttccatgagg cgcatgccgg
      721 tgccgtagcg caggcgcgcg acaaggccgg gcgtgccgtc gattttgccg agcagcgcgg
      781 tcacctcggc gcgggttaaa acgaccggga tgcgcttcga cggcttggcg cgggttatgg
      841 tatcgagcca gggcagggtg atgccaagga cttccttgta gaggaacagg atcgcgtgca
      901 tcgcctggtt ctgggtgctg gccgagactt cgcgcgcggt ggcgagccag gacagaaacg
      961 cctcaacctc ggccgcgccc atgtcgcgcg ggtggcgcat gccttgttgc cggatgaaaa
     1021 accggatcca gtagcagtag gtcttttcgg tggcgagact gtagtgcttg acgcggatgc
     1081 gttcgcgcac ctggtcaagc agctttggcg cttgaggcga caccgcggac gtgtcgcgtg
     1141 aacgggttgc gcgtgtcgcg gtttgttctt gcgtttcagt catggtgcgg gtctccgttg
     1201 atggccgcca agttatgcga catggcggcc agattgaatg acgtcagcct gtcgtagaat
     1261 tcaagttggg cgtctgcggc caccatgaag atcaactgct cgcgctccgc ttcaatcacc
     1321 gcgctcttgc tgccctcgtc cgcaaccgga gacgtgctcg ctcctgcggc cgccggtcgc
     1381 gctgcgggcg tttgtctcac ggctcgcttg cttcagtctc gcaccgcatc cgcttccagc
     1441 cacgcgaacg tcggcggctc ttgcgttcgt cggtggcgcg tcgcctgccg caagaacaca
     1501 aacgtcatct cgtcaaatca tgcgccgtcc aacattacgc tcaacggcgc tccctccggt
     1561 cgctggacgc tgcgcgataa ggccgcgcag cgcccgttag ctcaacgttg ggcgtcaaaa
     1621 tctcttcctc gtactcctcg tcgatcactc ggatctttac gtcaggcacg cgcggatagg
     1681 tgctgaagtc gtggttgagc aggccaatgc cgtcgtactc tcctcccgcg cctcctgttg
     1741 ctgcggtgcg ggccgcgtac ttcaccacat cgtcgatgct gtccacgtgc cacaatccct
     1801 ttcggaactc ggcaaggtat tcgtcctgcg tcatcccgcc gaagacagcc ggggtcagtt
     1861 cgacttcgat ttccttctcg atggtcacgc gcacagtgcg cttgatggat tccatgctca
     1921 tcactccgtg gttgtcgttt gcgcccaact ggcgcatcaa cccggacagc ctacggctgc
     1981 cggttatgct ggtcgttaga agtcacgccc cgcaccatcc gccatcgcag ccggcgagtt
     2041 caggttcggg ctccgtatcg cctcgcgccc acttcacgac cgccataatc ccgaccgcgc
     2101 ctttgtgccg cttcggcgag aacatcactc gcgggttacc attcgagtta atccccgcca
     2161 gtgtctcggc ggtcagaatc gttgtaatct tctcgtcggg cagcgcggca atctctttct
     2221 tccgtgcatt cacacacggg tagcactcct tgcttcggtg cggcagcggc tcaaacggcg
     2281 tctttgccag cagcgcgttt ctgtcgactt cggtgtggcg cacgagcggt gcatgcaggt
     2341 ttcgcccgcc gtgcttctcg ctttcctcta cccactcagg aaagttcgtg cggttgtcgc
     2401 tctcttcgcg ccgaatgccc accatgcaca ccacttcttt ttcagggtcc agcgcgtcaa
     2461 gccatgcgag cgccggttct tccttcagtg ccgcagtgca aaactggtac ttaccgcccc
     2521 cgccacgagg ccatgccttc ttgcgcatga ccaacgcttc catgccttcg ctcgcaattc
     2581 gcaccggctc aaagccaagc gtctgaaccc aggcttcggc tttcgtcact cgcgccggcc
     2641 accagtccgc agcccacccg gtgtcgctgt acgccaccgt cacatttttc aggccccgct
     2701 cgtgcgccca ctgaatcaga gcaatcgagt cgttcccgta actcgcaaat atcacgtagt
     2761 ccattgttct tttctttctc agaaaacttc taactactcg ttcgacggct ttaccttctt
     2821 cgccttcacg ctccataggt tgtacgtcgc gcgcttgttt ttctccgcaa caaactcggc
     2881 ggcagccttc tttgactcga acgcctcttc aaccgtaccc gcttcaaggt gatggccccg
     2941 cgcatatgga tgttgctggt gcttcattgc aagataaaca gtcgtcatgt tttctccgta
     3001 gttacgccgt cgaacccgtt ggtcaagccg accgtgggcc ggcgtaaggt ttttctcaac
     3061 tgccttcgcg cggccggctt accgtcgcgt tcatctcgac ctcttcaaca tccgatacat
     3121 cgtcgccctc gacaccccga actgctcccc cgcttcgcgc cccctgcccg tccgacgcgc
     3181 ttcgcgcagc gcctcgtcct tggcctttgc ctcttcgatc gtcgtcttgg cgatatattc
     3241 aattctcgcc ccgccccacc tcatcctcaa ttgccgctcg attgacaccg ccaccgcctc
     3301 cgtgaa
//
LOCUS       DBNGSB010000143        17508 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3782502, whole genome shotgun sequence.
ACCESSION   DBNGSB010000143 DBNGSB010000000
VERSION     DBNGSB010000143.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 17508)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17508)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17508
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3782502"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="AC159B_03965"
     CDS             complement(<1..917)
                     /locus_tag="AC159B_03965"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008538846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HIR3376939.1"
                     /translation="MWEVPKPKIEYAELSETYGKFVVEPLDRGFGVTLGNALRRILLS
                     AIPGAAVTSVKIESVLHEFSTISGVVEDVTQVILNLKELTLRLHTEKPKLLRLDVKGK
                     KDVTASDIQVDAEVDVLNPDLHIATLDRKDTRLAMELVVERGRGYVPAEKHRKSEHVI
                     GVIPVDSIFSPVQKVNFAVEDTRAGQATEMDRLVLEVWTDGSIRPEESIQEASKILID
                     SLKLFSGIGEEGEPFGGLEQGADAQMGKLYAMPIEELDLSVRPYNCLKRAGINTVGDL
                     MQRTEEEIVSVKNFGRKSLDEVKDKLTQLG"
     gene            complement(1030..1653)
                     /gene="rpsD"
                     /locus_tag="AC159B_03970"
     CDS             complement(1030..1653)
                     /gene="rpsD"
                     /locus_tag="AC159B_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014344628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HIR3376940.1"
                     /translation="MSRYTDAVCRLCRREGMKLYLKGEKCYTEKCPVHKRPVPPGMHG
                     QGRRKLSEFGIRLREKQKLRRIYGVFETQFKTYFRQAAQVKGITGVRLLQLLESRLDN
                     VVYRLGFATSRKEARQMVLHGHVAVNGKKITIPSFQVKPGHAITPTSRGREHPRVKEL
                     AQTGLRTLPSWLEFTVERLEGKVRGLPAREEIDVPVQEQLIVEYYSR"
     gene            complement(1650..2033)
                     /gene="rpsK"
                     /locus_tag="AC159B_03975"
     CDS             complement(1650..2033)
                     /gene="rpsK"
                     /locus_tag="AC159B_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HIR3376941.1"
                     /translation="MVQKGKKKRERRSIPVGVAHIQSTFNNTIVTITDAQGNVLGWSS
                     AGTTGFKGSRKGTPFAAGLAAEAAARKAMEHGVKQVEVFVKGPGAGREAAIRSLQAAG
                     LEVALIRDVTPIPHNGCRPPKRRRV"
     gene            complement(2088..2471)
                     /gene="rpsM"
                     /locus_tag="AC159B_03980"
     CDS             complement(2088..2471)
                     /gene="rpsM"
                     /locus_tag="AC159B_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018638456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HIR3376942.1"
                     /translation="MARIAGVDLPREKRVEIALTYIFGVGLARSREAMAATHVSADTR
                     VRNLTEEEVTRLREYIERNYKVEGDLRRDITMDIRRLVEIGAYRGLRHKKNLPVRGQR
                     TRTNARTRKGAKKTVGSGRRRLDKK"
     gene            complement(2485..2598)
                     /gene="rpmJ"
                     /locus_tag="AC159B_03985"
     CDS             complement(2485..2598)
                     /gene="rpmJ"
                     /locus_tag="AC159B_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006042225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HIR3376943.1"
                     /translation="MKVRASVRRMCEKCKVVKRGRRVFVICDNPKHRQKQG"
     gene            complement(2677..2895)
                     /gene="infA"
                     /locus_tag="AC159B_03990"
     CDS             complement(2677..2895)
                     /gene="infA"
                     /locus_tag="AC159B_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HIR3376944.1"
                     /translation="MPKKDAIEVEGTIAEVLPNAMFRVELPSGHRVLAHISGKMRINF
                     IRILPGDRVKVELSPYDPTRGRITYRYR"
     gene            complement(2920..3714)
                     /gene="map"
                     /locus_tag="AC159B_03995"
     CDS             complement(2920..3714)
                     /gene="map"
                     /locus_tag="AC159B_03995"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018703348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HIR3376945.1"
                     /translation="MQTKLRATGVEMIVIKTPAEIESMRRAGTLAAEALRAVARALRP
                     GIAGQELDKIGEDYIRARGGSPSFKGYRGFPGSICLSFNDEVVHGIPGQRSLKEGDII
                     SIDVGAEINGFHADLAGTFPVGKVSRDVARLLTVARESLYKAIDAVRPGKRLGDVGAA
                     VQAHVEAAGFNVVRDFAGHGIGRHLHEEPQIPNFGEPGTGQPLRVGMTLAIEPMVNAG
                     GYEVTMDDDGWTVRTKDHKPSAHFEHTVAVTDNAALVLTAIPDGAL"
     gene            complement(3719..4378)
                     /locus_tag="AC159B_04000"
     CDS             complement(3719..4378)
                     /locus_tag="AC159B_04000"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HIR3376946.1"
                     /translation="MNLVFLGPLGAGKGTQAQMLREREGIPQISTGDMLRQAMVEETD
                     LGRKARSFVDRGELVPDDVMIGLIDERLRQPDTRRGFVLDGFPRTLAQAEALDRVLRA
                     EGRTLDAVVYYKVSDETIISRLSGRRVCRKAAHIFHLDSHPPRVSGRCDVDGSELFQR
                     EDDKEDTVRHRLEVYHQQTEPLVEFYRSRGILEAIDAEADIDVVHERLRRILSERVET
                     H"
     gene            complement(4382..5650)
                     /gene="secY"
                     /locus_tag="AC159B_04005"
     CDS             complement(4382..5650)
                     /gene="secY"
                     /locus_tag="AC159B_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008538855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIR3376947.1"
                     /translation="MLGGLINSLRIPDLRRRVLFTAFMLVLFRLGSHLPVPGVDVSAL
                     ERLFRQQGSVFNFLDLFVGGAFSRFAIFALGVFPYITASIIMSLLQTVFPRLKEMVQE
                     EGEAGRRRVGMYTRYLTVVLAVLQAIGQTVLIRNLGALPDTRPLTMGMIVITLVTGTM
                     LLTWMGEIMTEYGVGNGVSLIIFGGIVARLPDQTARTLALVRVGEVAWYSFLADVLVV
                     LASIVAVVAITQAVRKIPVQYAKRVVGRRVYGGQATHLPLRLIQAGVIPIIFAVSVLQ
                     FPGTIAQFVRYEPIRRIGEMVLPGQPLGDALYFVLIILFTYFYTAVSFDPVEVSENIK
                     KYGGFVPGIRPGKPTSDYLSRVVERLTFVGALILAAIAVAPVYLARGTGVLTFYLAGT
                     SLLIVVGVALETMKQIEAYVLMRHYEGFMR"
     gene            complement(5631..6116)
                     /gene="rplO"
                     /locus_tag="AC159B_04010"
     CDS             complement(5631..6116)
                     /gene="rplO"
                     /locus_tag="AC159B_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011064541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HIR3376948.1"
                     /translation="MRIDDLRPARGSRRRRRRVGRGLGSGRGVYSGRGMKGQKARSGS
                     APRPGFEGGQTPLVKRLPFRRGVRAGGVSHAGGLRHPPFAPINLKALNVFETQTEVTP
                     ALLREKGLVRRNLRIKVLGTGTLDRALTIKAHAFSKSAAEAIEKAGGKAEVIRDARRP
                     D"
     gene            complement(6182..6706)
                     /gene="rpsE"
                     /locus_tag="AC159B_04015"
     CDS             complement(6182..6706)
                     /gene="rpsE"
                     /locus_tag="AC159B_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014416750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HIR3376949.1"
                     /translation="MVTKRVDPSTVNLSIEKAVSINRVAKVTKGAKRFNFSALVIVGD
                     EMGHVGVGLGKAAEVPDAIRKAVEDGKKSLISVPLRKTTIPHEVIGDFGASKVLLKPA
                     SEGTGVIAGGSVRAVLEAAGIKDILTKSLGSRNPINQARATLAALLELRQPEDVARTR
                     GRTVDDLAIRTAAR"
     gene            complement(6812..7180)
                     /gene="rplR"
                     /locus_tag="AC159B_04020"
     CDS             complement(6812..7180)
                     /gene="rplR"
                     /locus_tag="AC159B_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012268641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HIR3376950.1"
                     /translation="MLKRADRNILRQRRHLRIRRRMGGLPDRPRLSVYRSLNHIYAQI
                     VDDTKGATIIAASTRDPELRDSLKGKKKTEACALVGELVAKRAQAKGIKQVVFDRSGY
                     LYHGRIKALADAARKAGLQF"
     gene            complement(7212..7772)
                     /gene="rplF"
                     /locus_tag="AC159B_04025"
     CDS             complement(7212..7772)
                     /gene="rplF"
                     /locus_tag="AC159B_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HIR3376951.1"
                     /translation="MSRVGKAPIDIPKGVEVKVNGRTVEIKGPKGRLSRDVHADISVQ
                     TSDGTLVVQRPTDLEHHRALHGLTRALLANMVRGVTEGFKVELEIQGVGYRAAKQGNA
                     LALQVGYTHPVDVPPPDGIQFEVPQPNRIVVSGIDKELVGQVAARIRAIREPDPYKGK
                     GIRYAGERIKLKPGKAGRTVGGAGKA"
     gene            complement(7871..8272)
                     /gene="rpsH"
                     /locus_tag="AC159B_04030"
     CDS             complement(7871..8272)
                     /gene="rpsH"
                     /locus_tag="AC159B_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HIR3376952.1"
                     /translation="MGVITDPIADMLTRIRNANSAKHDHVDVPASRLKAEIAKILKDE
                     GYIGDFHRIERGPQGVIRISLKYGPRKESVLSGIRRVSKPGLRMYAQRAEIPRVRGGL
                     GVAILTTSRGVMTDKHARRIGIGGEVICYVW"
     gene            complement(8285..8470)
                     /locus_tag="AC159B_04035"
     CDS             complement(8285..8470)
                     /locus_tag="AC159B_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005239644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HIR3376953.1"
                     /translation="MPKKALINKWKRKPKFKVRAYTRCLRCGRPRAVFRKFGLCRICL
                     RQLAHKGEIPGLVKASW"
     gene            complement(8555..9079)
                     /gene="rplE"
                     /locus_tag="AC159B_04040"
                     /pseudo
     CDS             complement(8555..9079)
                     /gene="rplE"
                     /locus_tag="AC159B_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289317.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
     gene            complement(9253..9594)
                     /gene="rplX"
                     /locus_tag="AC159B_04045"
     CDS             complement(9253..9594)
                     /gene="rplX"
                     /locus_tag="AC159B_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HIR3376954.1"
                     /translation="MAVAVGRQRVHVKKGDTVEIVNGKHRGKRGKVLQVLTKDGKVIV
                     EGVNILKRHSKPTQKLPQGGIVEKEGAVFSGRVMLVCPKCSKLTRVGHGYLSDGTKVR
                     VCKQCGEQFEK"
     gene            complement(9596..9964)
                     /gene="rplN"
                     /locus_tag="AC159B_04050"
     CDS             complement(9596..9964)
                     /gene="rplN"
                     /locus_tag="AC159B_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HIR3376955.1"
                     /translation="MIQNYTRLKVADNTGARQLMTIRVMGGSRRRYASIGDIIVASVK
                     QAIPNSPVKQGEVVRAVIVRTTQPLRRPDGSYIRFDDNAAVILTDAQNPKGTRIFGPV
                     ARELRDKQFMKIISLAPEVL"
     gene            complement(9961..10215)
                     /gene="rpsQ"
                     /locus_tag="AC159B_04055"
     CDS             complement(9961..10215)
                     /gene="rpsQ"
                     /locus_tag="AC159B_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HIR3376956.1"
                     /translation="MERARRKTRVGLVVSDKMLKTVVVKVETTSRHPLYGKTLRKVKK
                     FKAHDERSEAHPGDKVLIAETRPISKDKRWRVIQVLEKAK"
     gene            complement(10230..10421)
                     /gene="rpmC"
                     /locus_tag="AC159B_04060"
     CDS             complement(10230..10421)
                     /gene="rpmC"
                     /locus_tag="AC159B_04060"
                     /inference="COORDINATES: protein motif:HMM:NF013027.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HIR3376957.1"
                     /translation="MESSELREMTPDELMKKLEDMQQELFFLRARIAGQTPNPAKIMQ
                     ARRDVARLKLALAEKGVRV"
     gene            complement(10424..10843)
                     /gene="rplP"
                     /locus_tag="AC159B_04065"
     CDS             complement(10424..10843)
                     /gene="rplP"
                     /locus_tag="AC159B_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015152295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HIR3376958.1"
                     /translation="MLMPKRTKFRKAHRGRMKGRPLAGSYIAFGEFGLQAMEPAWLSS
                     AQIEAARRAIMHYIKRGGKLWIRIFPDKPVTKKPAETRMGSGKGNPEFWVAVVHPGRI
                     LFELAGVDEANAREAMTLASHKLPIKTKFSKREEATV"
     gene            complement(10974..11630)
                     /gene="rpsC"
                     /locus_tag="AC159B_04070"
                     /pseudo
     CDS             complement(10974..11630)
                     /gene="rpsC"
                     /locus_tag="AC159B_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011876610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
     gene            complement(11743..12081)
                     /gene="rplV"
                     /locus_tag="AC159B_04075"
     CDS             complement(11743..12081)
                     /gene="rplV"
                     /locus_tag="AC159B_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HIR3376959.1"
                     /translation="MEARAVAKYVKMSPLKVRRVAQMIRGQRVSEAQAMLKFTQTRAA
                     RVIEKVLNSAVANAENNLELDRDELVVARAYVDKGPSMKRMTPRARGRADILTKRSSH
                     VTVIVADRGK"
     gene            complement(12085..12450)
                     /gene="rpsS"
                     /locus_tag="AC159B_04080"
     CDS             complement(12085..12450)
                     /gene="rpsS"
                     /locus_tag="AC159B_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HIR3376960.1"
                     /translation="MGRSTRKGPFVDEHLAKKIRELNRGREKRVIKTWSRRSTIVPDM
                     VGHTIAVYDGRKHIPVYVSEQMVGHKLGEFAPTRIFKGHGVAGAAAAAAAAAERTTGV
                     TPAATGTAAPSAAAPAKGS"
     gene            complement(12508..13332)
                     /gene="rplB"
                     /locus_tag="AC159B_04085"
     CDS             complement(12508..13332)
                     /gene="rplB"
                     /locus_tag="AC159B_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HIR3376961.1"
                     /translation="MGLKKFKPTSAGRRFMTVSTFEDLAKKEPERGLVVPMRKTGGRN
                     AQGKVTARHIGGGSKRMYRLIDFKRNKDGVGAKVAALEYDPNRSARIALLQYQDGEKR
                     YILAPVGLNVGDTVQSGPDAEIKPGNAIPLRHVPIGTMIHNIELSPKRGGQMVRAAGA
                     AAQVMAKEGEFAHLRLPSGEVRLVALDCKATIGQVSNLDHDAVTGGKAGRTRWRGRKP
                     TVRGVAMDPASHPHGGGEGRSPIGMPGPVSPWGQPTLGYKTRKAKPSDQYIVKRRK"
     gene            complement(13346..13633)
                     /gene="rplW"
                     /locus_tag="AC159B_04090"
     CDS             complement(13346..13633)
                     /gene="rplW"
                     /locus_tag="AC159B_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HIR3376962.1"
                     /translation="MIDVRHIIRRPILTEKTTRGTEIGKYTFEVLDGVNKLDIKKAVE
                     ELFNVTVTKVNTVKIHGRMKRRGQHRYGAAGYRKAIVTLAPGQKIDLENLT"
     gene            complement(13630..14253)
                     /gene="rplD"
                     /locus_tag="AC159B_04095"
     CDS             complement(13630..14253)
                     /gene="rplD"
                     /locus_tag="AC159B_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HIR3376963.1"
                     /translation="MPTAQLFDAKGKETGKIDLPDQVFGIKPHRAVLHEVLVAQLASR
                     RRGTHATKTRGEVSGSTRKIYRQKGTGRARHGSNKAPIFVGGGITFGPRPRDHSHDTP
                     KKVRRLALRSALSAMAAAGRIVVIEPPDLETPKTRTVAEMLDRIAIDGKALVVTAGSD
                     VALSRAAANLDGVKVTPSQRLNVHDLLTAKYVVLTRPALDAVTEALE"
     gene            complement(14259..14891)
                     /gene="rplC"
                     /locus_tag="AC159B_04100"
     CDS             complement(14259..14891)
                     /gene="rplC"
                     /locus_tag="AC159B_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HIR3376964.1"
                     /translation="MTAIVGRKVGMSQIFDDAGNIVSVTVIEAGPCVVTNVRTPERDG
                     YAAVQVGFESIAERKVNKPLKGVFAKAKTAPRKVLREFRTSGENFTVGQEIKVDTFGG
                     GDVVDVTGTTKGKGFSGAMKRHNFKGQRDTHGVSLMHRAVGSIGISDVARVWPGKRMP
                     GRHGGERVTVRGLKVMRVDAERNLLMLKGAIPGPRGSLIVIRKQQAKKAR"
     gene            complement(14924..15229)
                     /gene="rpsJ"
                     /locus_tag="AC159B_04105"
     CDS             complement(14924..15229)
                     /gene="rpsJ"
                     /locus_tag="AC159B_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014182791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HIR3376965.1"
                     /translation="MAQRIRIKLRAFDHKALDQSAERIVETVRRTGARVSGPVPLPTD
                     RNLFCVIRSPHIDKESMEHFELRTHKRLIDILEPTQKTVDALSHLDLPAGVDIEIKL"
     gene            complement(15232..16455)
                     /gene="tuf"
                     /locus_tag="AC159B_04110"
     CDS             complement(15232..16455)
                     /gene="tuf"
                     /locus_tag="AC159B_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HIR3376966.1"
                     /translation="MAKQKFERTKPHVNIGTIGHVDHGKTTLTAAITHTLAGVGVTQA
                     KGYADIDNAPEEKARGLTINISHVEYESPKRHYAHIDAPGHADYIKNMITGAAQMDGA
                     VLVVSAADGPMPQTREHVLLARQVNVPYIAVFLNKIDMVDDPELLELVEVEVRDLLTK
                     YQFPGEKTPIVRGSALKALEALEGAKGKGIARGQNEWVDKIWELVEAVDSYIPTPQRE
                     VDKPFLMAVEDIFTITGRGTVATGRVERGKVKVGEEVEIVGLRPESKRTVVTGIEMFR
                     KTLDEAVAGDNVGVLLRGVEKEEIERGMVLAKPGSIKPHTKFKAEVYVLTKEEGGRHT
                     PFFTGYRPQFYFRTTDVTGDIKLPDGMEMVMPGDNVNMDVALITPVALEEGLRFAIRE
                     GGRTVGAGVVTKVTA"
     gene            complement(16551..16626)
                     /locus_tag="AC159B_04115"
     tRNA            complement(16551..16626)
                     /locus_tag="AC159B_04115"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16591..16593),aa:Thr,seq:ggt)
     gene            complement(16891..16965)
                     /locus_tag="AC159B_04120"
     tRNA            complement(16891..16965)
                     /locus_tag="AC159B_04120"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16931..16933),aa:Cys,seq:gca)
     gene            complement(17059..17133)
                     /locus_tag="AC159B_04125"
     tRNA            complement(17059..17133)
                     /locus_tag="AC159B_04125"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17099..17101),aa:Gly,seq:gcc)
     gene            complement(17153..17240)
                     /locus_tag="AC159B_04130"
     tRNA            complement(17153..17240)
                     /locus_tag="AC159B_04130"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17205..17207),aa:Leu,seq:taa)
     gene            complement(17302..>17508)
                     /locus_tag="AC159B_04135"
     CDS             complement(17302..>17508)
                     /locus_tag="AC159B_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HIR3376967.1"
                     /translation="VRLITGPDHRRLLPAATLAGGITLLLADTVARTILAPTEIPVGI
                     VTAGLGAPFFLYLLRRNRTRTYWS"
BASE COUNT         2959 a         5931 c         4906 g         3712 t
ORIGIN      
        1 ccaagttgtg tcagcttatc cttcacttcg tcaagcgatt tccgcccaaa attcttgacg
       61 ctcactatct cttcttccgt ccgctgcatc aaatcgccga cggtgttgat cccggcccgc
      121 ttcaggcagt tatagggacg gaccgaaaga tccagctcct cgatgggcat cgcatacagc
      181 ttgcccatct gagcatcggc gccttgctcc aggccgccga agggttctcc ctcttcgccg
      241 atcccggaga agagcttgag cgaatcgatc aggatctttg aggcttcctg aatcgactcc
      301 tcaggacgga tcgaaccgtc ggtccagact tcgagcacca gccggtccat ctccgtggcc
      361 tgacccgccc gggtgtcctc aacggcaaag ttgaccttct gcaccggtga gaagatggag
      421 tcgactggga tgacgccgat cacgtgctcg ctcttgcggt gcttttccgc cggaacatat
      481 cccctgcctc gctccacgac cagttccatc gccaggcgtg tgtccttccg gtcgagtgtc
      541 gcgatgtgca ggtcaggatt cagcacgtcg acttcagcgt ccacctgaat gtccgacgcc
      601 gtgacgtcct tcttgccttt gacgtccagg cgcaggagtt tgggtttctc ggtatgcagg
      661 cgaagggtga gctccttgag attcagaatg acctgcgtca catcctcgac cactcccgag
      721 atcgtggaaa attcatgcag cacgctctcg atcttgacgg aggtcaccgc cgccccagga
      781 atcgcggaga gcaaaatacg ccgcaacgcg ttgcccagcg ttacgccaaa cccgcggtca
      841 agcggctcga cgacaaactt tccatacgtt tccgacagtt cggcgtactc gatcttcggc
      901 ttcggaactt cccagattgt ggcaatgctc atcctaaaac ctcctgattt cgaattgcgg
      961 atttacgatt tcggattcaa tacctgcgaa ttcaatccgc aattcacaat ccgaaatccg
     1021 aaattgctct tagcgtgagt agtattcaac aatgagctgc tcttgcacgg gaacgtcgat
     1081 ctcctcgcgt gcaggcaggc cgcgcacttt gccttcgagc cgttccactg tgaactccag
     1141 ccagcttggc agggtccgca gcccggtctg agcaagctcc ttgacgcgcg ggtgctcgcg
     1201 tccgcgcgaa gtcggggtaa tcgcgtgtcc cggcttcact tggaacgacg ggatcgtgat
     1261 tttcttccca ttcacggcga cgtgcccgtg cagcaccatc tgtcgggctt ccttccggga
     1321 cgtggcgaaa cccaggcggt agacgacgtt gtcgagcctc gactcgagca actgcagcag
     1381 gcggacgcct gtgatgccct tgacctgtgc ggcctgccgg aaataggtct tgaactgcgt
     1441 ctcaaatacc ccgtagatgc ggcgcagctt ttgcttctcg cgcaggcgga tcccgaattc
     1501 actcagcttc cggcggccct gcccatgcat gcccggtgga acggggcgct tgtgtacggg
     1561 acacttttcg gtgtagcact tctcgccttt gaggtagagc ttcatcccct cccggcggca
     1621 caaacggcag accgcatccg tgtagcggct catactcgcc tccgcttcgg gggtctgcac
     1681 ccgttgtgcg gaattggtgt cacgtctcgg atgagcgcaa cctcgagccc ggcggcctgc
     1741 agcgaacgaa tcgcggcctc gcggcccgcg ccggggccct tcacgaacac ctcgacctgt
     1801 ttcacgccgt gctccattgc ctttctggcc gcggcctccg cggccaaccc ggcggcaaac
     1861 ggcgtccctt tgcgcgagcc cttgaacccg gtcgtccccg cgctcgacca ccccaggacg
     1921 ttaccctgag cgtcggtgat cgtcacgatg gtgttattga aggtggactg aatgtgcgcc
     1981 acccccacgg ggatcgatct gcgctctctc ttctttttgc ctttctgtac cattcgttcg
     2041 ctcccgttcg cgcgtccgcg cttgcgcgtt tgcgcgcttg cgtaatttta cttcttgtca
     2101 agtctgcggc ggccagagcc cacggttttc ttggcgcctt tccgcgtccg tgcgttggtc
     2161 cgggtccgct gacctcggac cggcagattc ttcttgtggc gcagcccgcg gtaggcgccg
     2221 atctcaacga gacgcctgat gtccatcgtg atgtcccggc gcaggtcgcc ctcaaccttg
     2281 tagttgcgtt cgatatactc gcggagccgg gtcacttctt cctcggtgag attgcgcacc
     2341 cgagtatccg cgctcacgtg agtggcggcc atcgcttcgc gcgaccgcgc cagccccacg
     2401 ccgaagatat aggtgagagc aatctccacc cgcttctcgc gaggcagatc aacgccggct
     2461 atacgcgcca tttctcttct ctcctcagcc ttgcttttgc ctgtgcttag gattgtcgca
     2521 aatcacaaac acgcgccgtc cgcgcttgac caccttgcac ttttcgcaca ttcttcgcac
     2581 tgatgctcgt actttcatga cctaacctct cagattgctt cgtcgcttcg ctcctcgcaa
     2641 tgacgggagt cgttggcttt ccggccagtc ggccggctac ctatacctat acgtgatccg
     2701 ccctcttgtc ggatcgtagg gtgacagttc caccttcacc cggtctcccg gaaggatccg
     2761 gatgaagttg attcgcatct tgccggagat gtgcgcaagc acgcggtggc cgcttggcag
     2821 ctccacgcgg aacatcgcgt tgggcagcac ctcggcgatc gtcccctcga cctcgattgc
     2881 gtccttctta ggcatcgttc aaaccttcct attatacatt tacagggccc cgtcgggaat
     2941 cgcagtcagg accagcgccg cattgtccgt caccgcgacc gtgtgctcga agtgggccga
     3001 gggtttgtgg tctttcgtcc gaacggtcca cccatcgtcg tccatcgtga cctcgtaccc
     3061 acccgcgttc accatcggct caatcgccag ggtcatccca acccgcagcg gctggcctgt
     3121 ccccggctcg ccgaagttgg ggatctgcgg ttcttcatgc aggtgccggc cgatcccgtg
     3181 cccggcaaag tcccgcacga cattgaagcc ggcggcttcc acatgcgcct gcactgccgc
     3241 ccccacgtcg ccgagccgtt ttcccggccg gacggcgtcg atggctttgt agagcgactc
     3301 ccgggccacg gtgaggagtc gcgccacgtc gcgcgagacc ttaccgacgg ggaatgtccc
     3361 ggcgaggtcg gcgtgaaacc cgttgatctc cgctccgaca tcgatcgaga tgatgtcgcc
     3421 ctccttcaga gagcgctggc cggggatgcc atgcacaacc tcatcgttga atgaaaggca
     3481 tatgctcccg ggaaagcccc ggtaaccctt gaacgacggc gagccaccgc gcgcccgaat
     3541 gtaatcttca ccgatcttgt ccagttcttg cccggcgatt ccggggcgca gggcacgggc
     3601 gaccgcgcgc agcgcttcgg cagccagggt tcccgcacgg cgcatgcttt cgatctcggc
     3661 cggggttttg atgacgatca tctccacccc ggtcgcacga agcttggttt gcaccgcact
     3721 aatgcgtctc aacgcgctcg ctgaggatcc tgcgcagacg ctcgtggact acgtcaatgt
     3781 ccgcttccgc gtcgatggct tccaggatcc cgcggctgcg gtagaactcc accagcggct
     3841 ccgtctgctg gtggtaaacc tccagccggt gccggacggt gtcttccttg tcgtcctcac
     3901 gctgaaagag ctcgctgccg tcgacatcac agcgacccga tacgcgcggg gggtgtgaat
     3961 ccaggtgaaa gatgtgggct gctttccggc agacccgccg cccgctcaac cggctgatga
     4021 tcgtttcatc gctgaccttg taatacacga cggcatcgag cgtccgtccc tcagcccgta
     4081 gaaccctgtc taatgcctcc gcctgcgcca gcgtgcgcgg aaaaccgtcg aggacgaatc
     4141 cgcggcgcgt atcgggctgc ctgaggcgct cgtcgatcag cccgatcatc acgtcatcgg
     4201 gcacaagctc tccccgatca acgaacgacc tggccttgcg ccccaaatcc gtttcttcga
     4261 ccatcgcctg gcgcagcatg tctcccgtgg agatctgcgg aattccctcg cgctcccgca
     4321 gcatctgggc ctgcgtgcct ttgccggcgc ccaatggacc gagaaacacg aggttcacaa
     4381 cttacctcat gaacccttcg tagtgccgca tgagcacata ggcctcgatc tgtttcatcg
     4441 tctccagcgc gacgccgacc acgatgagca gcgacgtgcc tgccaggtag aacgtgagca
     4501 cgccggtgcc gcgggcgagg tagaccgggg caaccgcgat ggcggccagg atgagcgcac
     4561 cgacgaacgt caaccgctcg acgacgcgcg agaggtagtc gctggtcggt ttgcccgggc
     4621 gaatcccggg aacaaatccc ccgtacttct tgatgttctc cgacacctcg actgggtcga
     4681 agcttactgc ggtgtagaaa tacgtgaaca gaatgatgag aacgaagtat aacgcgtcac
     4741 ccaggggctg tccgggtaac accatttctc cgatcctgcg gatcggttca taccggacga
     4801 actgggcaat cgtaccggga aactgcagca ccgagaccgc gaagatgatc ggaatgacgc
     4861 cggcctggat gagccgcagc ggcagatgcg tcgcctgccc gccgtagacg cgcctgccga
     4921 ccacgcgctt cgcatactgc accggaatct ttcggacggc ctgcgtgatg gcaaccacgg
     4981 ccacgatgct ggccagcacg accagcacat ccgcgaggaa cgagtaccat gcgacctcgc
     5041 cgaccctgac cagggcgagg gtgcgggccg tctgatcggg taaccgcgcg acaatgccgc
     5101 cgaaaatgat gagcgagacg ccattcccca cgccgtattc ggtcatgatc tcgcccatcc
     5161 acgtcagcag catcgtgccc gtgacgagcg tgatcacgat catgcccatc gtcagcggtc
     5221 gcgtgtcggg gagggcgccc aggtttcgga tcaggacggt ctggccgatg gcctggagca
     5281 ccgcgaggac caccgtcagg taccgggtgt acatgccgac ccggcgccgg cctgcctcac
     5341 cttcctcctg caccatctct ttgaggcggg ggaagacggt ctgcagcaag ctcatgatga
     5401 tggatgcagt gatgtatgga aacacgccca gcgcaaagat cgcgaaccgg ctgaacgcgc
     5461 caccgacgaa gagatcgaga aaattgaata cactcccctg ctgccggaag aggcgctcca
     5521 gcgcagacac gtccacgcca ggcaccggca gatgggagcc aaggcggaac agcacgagca
     5581 taaaggcagt aaagagcacc cgccgccgga ggtccgggat tcgaagcgag ttaatcaggc
     5641 cgccgagcat cccgtatcac ctcagccttg ccgcctgcct tttcaatggc ctcggccgcg
     5701 ctcttgctga acgcatgggc cttgattgtg agcgcacggt cgagcgtgcc cgtgcccaga
     5761 actttaatac gtagattgcg cctcaccaga cccttctcac gcaggagggc cggcgtcact
     5821 tcggtctgcg tctcaaacac attgagcgct ttcaagttga tgggggcaaa cgggggatgc
     5881 cgcagcccgc cggcgtgact gaccccgccg gcgcgaacac ctcgccggaa cggcagccgt
     5941 ttcaccagcg gagtctgtcc gccttcaaat ccaggtcggg gcgcagagcc tgaccgcgcc
     6001 ttctgacctt tcatcccgcg gccgctatac acaccacggc ccgatccaag accgcggccg
     6061 acacgccggc gccttcgccg cgagcctcgc gcaggcctca ggtcatcgat cctcaccatc
     6121 ggcactttct ctgcgtattt cttcttgacc atttcagggc tccactctgt tggctaccca
     6181 gctacctggc cgccgtccgt atcgccagat catccacggt ccgcccacgt gttcttgcaa
     6241 cgtcttcggg ctgacgcaat tccagcaacg cggcaagcgt cgctcgcgcc tgattgatgg
     6301 gattccgcga accaagcgat ttggtgagga tatccttgat gcccgccgcc tcgagcacag
     6361 cgcggaccga tccaccggcg atgaccccgg tgccctccga cgccggcttc agaagaacct
     6421 tcgatgcgcc gaagtcgccg atgacttcgt gcgggatcgt tgtcttgcga agcggcacgc
     6481 tgatgagcga cttcttaccg tcctcgacgg ctttgcggat ggcatcggga acctcggccg
     6541 ctttcccgag cccgacgccg acgtgtccca tctcgtcgcc aacgatgacc agcgcgctga
     6601 aattgaaccg tttggcgccc ttcgtcacct ttgcgacacg gttgatggac acagctttct
     6661 cgatgctcaa atttacggtc gaaggatcca ctctttttgt gaccattagt gcctccgcac
     6721 ttcgtcttcc ctgtctaccc ggtctcccca gtccacccag tctcaatcaa gtaaacgggg
     6781 aagaccggga agacggggca gactgggtag actagaactg caatcccgcc ttacgggccg
     6841 cgtccgcgag cgctttgatc cggccgtggt acaggtagcc gctgcgatcg aagacgacct
     6901 gcttgatacc tttcgcctgc gcacgcttgg cgaccagctc cccgacgagt gcacacgcct
     6961 cggtcttctt cttgcccttc agggaatcgc ggagctccgg atcgcgcgtt gatgccgcga
     7021 tgatcgtcgc gccctttgtg tcatcgacga tctgggcgta gatatgattc aggctgcggt
     7081 agaccgacag acgcggccga tccggcaggc cgcccatccg cctgcgaatc cgcaggtggc
     7141 ggcgctggcg cagtatgttg cgatcggctc tctttaacat caaaatcccc tcacgactct
     7201 cgtcattttg cttaggcttt gcccgcgcca ccgaccgtgc ggccggcctt acccggcttg
     7261 agtttgatgc gctctcccgc gtaccggatg cccttgcctt tgtatggatc cggttcacgg
     7321 atggccctga tgcgcgcggc gacctgcccg acgagttcct tgtcaatacc gctgacgacg
     7381 atgcggttcg gctgcggaac ctcgaactgg atgccatcag ggggcggcac atcgaccgga
     7441 tgcgtgtagc ccacctgcag ggccagcgca ttgccctgct tggcggcacg gtaaccgacg
     7501 ccctgaatct caagctccac tttgaatccc tcggtcacac ctctgaccat gttcgccagc
     7561 agggcacggg tcaaaccatg cagggcgcgg tggtgctcga gatccgtggg ccgctgtacg
     7621 acgagggtac cgtctgacgt ctgcacgctg atatcggcgt gcacgtcacg gctcaggcgc
     7681 cccttcgggc ctttgatttc caccgtccga ccgttgacct tgacctcgac gcccttcggg
     7741 atgtcgatgg gcgccttgcc tacacgggac atcttagacc cccaactgcg gtcgtaggtc
     7801 aagggtcgta ggtcgtagca aagacaaacg aagttcccac gaccaacgac ctacgaccga
     7861 cgaccgcctt ttaccagaca taacaaataa cctctccgcc gatcccgatg cgccgcgcgt
     7921 gcttatcggt catgacgcct cgcgacgtgg tcaagatggc aacacccagg ccgccccgca
     7981 cccgggggat ttccgcgcgc tgcgcgtaca tccgcagccc gggcttgctg acccgccgga
     8041 tgcccgacag caccgattct ttgcgaggtc cgtacttgag actaatccgg atgacgccct
     8101 gcggcccgcg ctcaatgcga tgaaaatcgc caatgtaccc ttcatccttc aggatcttgg
     8161 caatctccgc cttgagtctg ctggccggca cgtcgacatg gtcatgcttg gcagagttcg
     8221 cgttgcgaat tcgtgtcagc atgtcagcga tcggatctgt gataacgccc acttgataac
     8281 ctccctacca gcttgccttt acgagtccgg ggatctcgcc cttatgcgcc agctgccgca
     8341 gacaaatccg gcagagccca aacttgcgga ataccgcgcg ggggcgcccg cagcgcaggc
     8401 agcgcgtgta cgctcgcacc ttgaacttcg gcttccgctt ccacttgttg atcaaggcct
     8461 tcttgggcac ttgcttcctc ccttagttcg gtcgtaggtc gtcggtcgta gatcgtggac
     8521 gacccaccac cgccttctcg cgttctgcgg ctcctgccaa cggcaatccc atcagcttga
     8581 gaaactctct cgcttcttca tcggtcttcg ccgtagtgac cagcgtgatg tccatcccac
     8641 ggatcttctc gatcttgtcg tactcgatct ccggaaagat cagctgctcg cggagcccga
     8701 tgttcagatt cccgcgcccg tcgaactgcc gctccgagat tcccttgaaa tccttgatgc
     8761 gcggcaagga gatgctgaac agcttgtcga cgaactcgaa catgcggtcc ccgcgaagcg
     8821 ttaccttggc cccgatcggg atacccttcc gcagtttgaa ggccgcgatc gacttccgcg
     8881 cgcgcgcgat catggggcgc tgtccggcga tcaccgcaag ctcctcgaca gccttatcga
     8941 caaaacgcgc gtcctgcgtc gcttcaccaa cccgcacgtt gatgacaacc ttctcgagcc
     9001 gcgggacttc catcacgttc ttgtagctga aatgctcccg cagcttcgga acgacatcgt
     9061 tccgatatcg ctccctcagc ctcgccggtg ctttaggagc cttcggcgtc ttggcaactt
     9121 ctggggcggc cttgccagct tctggaacct tggcagcttc tggggccttg gcagctcctg
     9181 gggccttggc agctcctggg gccttggcag cctctgattt tgtttgcgcg cttgcgcgct
     9241 tgcgcgtctc tttcatttct caaactgctc cccgcattgt ttgcaaacgc gtactttcgt
     9301 cccgtcgctc aggtagccgt ggccgacccg cgtgagcttg ctgcactttg gacagaccag
     9361 catcacccga ccgctgaaca cggccccctc cttctcgaca atgccgccct gcggcaactt
     9421 ctgcgtcggc ttgctgtgcc gcttaaggat gttcacccct tcaacgatga cctttccatc
     9481 tttggtcagg acctggagca ccttgccacg tttgccccgg tgcttcccgt tgacgatctc
     9541 taccgtatcg cctttcttta catggactct ctgtcgaccg actgcgactg ccatgttaga
     9601 gaacctccgg agccagcgag atgattttca tgaactgttt gtcccggagc tcgcgggcga
     9661 ccggaccgaa gatgcgcgtg cccttcggat tttgcgcgtc ggtcaggata acagcggcat
     9721 tgtcgtcgaa ccggatgtat gagccgtcgg gccggcgcag tggctgcgtg gtgcgcacga
     9781 tcaccgcccg cacgacttcg ccctgcttga ccgggctgtt ggggatcgcc tgtttcaccg
     9841 acgcgacgat gatgtcgccg attgaggcgt agcgccgccg gctgccgccc atcactcgga
     9901 tggtcatcag ctgcctggcg cccgtgttat cggccacctt cagccgcgtg tagttctgaa
     9961 tcatttcgct ttctccagca cctggatgac ccgccaccgt ttatccttgc tgatcggtcg
    10021 ggtctctgcg atcagcacct tgtcgcccgg gtgcgcctcg ctgcgctcgt cgtgagcctt
    10081 gaacttcttc acttttcgga gggtcttgcc gtacagcgga tgtcgtgagg tggtctccac
    10141 cttcacgaca acggtcttca gcatcttgtc gctgacgaca aggccgacgc gcgtcttgcg
    10201 tctcgccctc tccatcgttt ccattgcgct cacactctca cgcccttttc ggccagcgcc
    10261 agcttgaggc gcgccacatc gcgtcgcgcc tgcatgatct ttgccggatt gggtgtctgc
    10321 cccgcaatcc gcgcacgcag aaagaacaac tcctgttgca tgtcttcgag cttcttcatc
    10381 agctcatcgg gtgtcatctc gcggagttcc gaggactcca tggctagacc gttgcctctt
    10441 cgcgcttcga gaacttggtc ttgatgggca gcttgtgcga ggccagcgtc atcgcttccc
    10501 gggcattcgc ctcatcaaca ccggccagct cgaacaggat ccggccggga tgcaccactg
    10561 ccacccagaa ctctgggttc cctttgccgc tccccatccg ggtctcggcc ggcttcttcg
    10621 tcaccggttt gtcaggaaaa atccggatcc agagtttccc gccgcgcttg atgtagtgca
    10681 tgatcgcccg ccgcgccgct tcaatttggg cgctcgagag ccaggcgggc tccatggcct
    10741 ggagcccgaa ctcaccgaat gcgatatagg aacctgccag ggggcggccc ttcatacgac
    10801 ctcggtgcgc tttgcggaat ttagtgcgtt taggcatcaa cattggaatc tctcctggtc
    10861 acaacctgac gcggggtctt cgtcggaata acgacgggac catcggcccc gccagagacc
    10921 ggttcggttg ccatatgccg ctccaagggg cggggccgac gggtctccaa ctcctggcgc
    10981 cgttcgggta gaatctcgcc gcgatagatc cagaccttta cgccgatgcg accgtaggtc
    11041 gtaagcgact ctgcaacgcc aaagtcgatg tcagcacgca atgtatggag cggcactcgg
    11101 ccctcgcggt accattcgta acgcgcgatc tccgagccgc ccagccggcc gctggccgag
    11161 atgcgaatgc ccttcgcgcc cgagcggagc gatcgctgca cagcctgctt catggcccgg
    11221 cggtaggcaa ctcgccgctc cagttgctgc gcgacgttct cagcgaccag aatggcctca
    11281 agctcgggtc ggcggatctc ctgcacgttg agctgaacct gtttgccggt catctcatcg
    11341 agctctttct tcaacgcatc aatacccgta ccgccgcgtc cgataatgat gccggggcgc
    11401 gccgcatgga tcaccacgcg cacgcgcggc ttctcaccga cgcccgactg gctcccgaac
    11461 cgctcgatct cgacgcgcga gatgcctgcg cggtagagtt tcttcttgat gtgggtgcgg
    11521 atcttgatgt cttccaggat cagcggcgcc atcttcttgc tgtaccaccg cgactcccag
    11581 tcgcggatga tcccgacccg aaaccctatt ggatggatct tttgacccaa ctacatcacc
    11641 tccgactgcg tctccccagt ctcccccgtc ttcccagtct cccccgtctc actacaccag
    11701 ctttgagacg gggcagccgg ggtagacagg gaagacgatt cactatttcc ctcgatccgc
    11761 gacaattacg gtgacgtggc tgctgcgttt cgtcaaaatg tcggcccggc cgcgggcgcg
    11821 gggcgtcatg cgtttcatcg acggtccctt gtccacgtac gcgcgcgcga ccacgagctc
    11881 gtcccggtcg agttcgaggt tgttctcggc gttggcgacc gcggagttca agactttctc
    11941 aatcacgcgg gcggcgcgcg tctgcgtgaa tttcaacatg gcctgggcct cgctcacacg
    12001 ctggccgcgg atcatctgcg cgacccgccg gacttttagc ggagacatct tcacgtactt
    12061 ggccacagcc ctcgcttcca tgtcttagga ccctttagcc ggcgcggcgg cgctcggtgc
    12121 cgcggttcct gtcgccgccg gcgtcacgcc ggtcgtgcgc tcggccgccg cggccgctgc
    12181 tgcggccgca cccgccacgc cgtgcccctt gaagatccgg gtgggggcga actctccgag
    12241 cttgtggccg accatctgct cggacacgta gaccggaatg tgtttgcgcc cgtcgtaaac
    12301 ggcgatcgta tgaccgacca tgtcggggac aatcgtcgag cgccgcgacc aggtcttgat
    12361 gaccctcttc tcgcgtccgc gattcaactc gcgaatcttc ttggcgagat gctcgtcgac
    12421 gaacggcccc ttccttgttg atcgtcccat ctggttgctc cctaaactcc gatagcgcct
    12481 gcgcgtttcc gcgctcccgc gctcccgtta tttccttcgt ttcacgatgt actgatcgct
    12541 cggcttagct ttgcgtgtct tgtagcccag cgtcggctgc ccccatgggc tcaccggacc
    12601 cggcatgcca atcggcgagc ggccctcacc gccgccgtgc gggtggctgg cggggtccat
    12661 cgcgacacca cgcactgttg gtttccgtcc acgccagcgt gtccggccgg ctttgccgcc
    12721 ggtgaccgca tcgtggtcga gattgctcac ctgtccgatc gtggccttgc aatcgagcgc
    12781 gacaaggcgc acttcgccag acggcaggcg taggtgcgcg aattcaccct ctttggccat
    12841 cacctgtgcc gctgcgcccg ccgcgcggac catctgaccg ccgcgtttgg gggatagctc
    12901 gatgttatga atcatggtgc cgatcggcac gtggcggagg ggaatggcgt tccccggctt
    12961 gatctccgca tccggcccag actgcacggt gtcgccgacg ttgagcccaa cgggcgccag
    13021 gatgtagcgc ttctcaccgt cctgatactg cagcagggcg atgcgcgccg agcgattcgg
    13081 atcgtactca agcgcggcca ccttcgctcc gaccccatct ttgttgcgtt tgaagtcgat
    13141 gagccggtac atccgtttgc tgccgccgcc gatgtggcgc gcggtgacct tcccctgcgc
    13201 gttccggccc ccggtcttgc gcatgggcac gaccaaccca cgctcgggct ccttcttggc
    13261 cagatcctca aaggtggaca cggtcatgaa ccgccgtccc gccgatgtcg gcttgaactt
    13321 ctttagaccc attgcttgcc tccggctacg tcaggttctc gaggtcgatc ttctggccag
    13381 gcgccagcgt tacgatcgcc ttgcggtacc cggccgcgcc gtatcggtgc tggccgcggc
    13441 gcttcatccg gccgtgaatc tttaccgtgt tcaccttggt cacggtcacg ttaaacagct
    13501 cctcaacggc cttcttaata tcgagtttgt tcaccccatc gaggacctcg aaagtgtact
    13561 tgccgatttc cgtcccccgc gtggtcttct cggtcaggat cggacgccgg atgatgtgac
    13621 gtacatcaat cattccaggg cctccgtgac cgcatccagc gcgggacgcg tcagcactac
    13681 gtacttagca gtcagcaagt catgcacgtt gaggcgctgc gatggggtga ctttcacccc
    13741 atccagattg gccgcggccc gtgagagcgc gacatcgctg ccggccgtta cgaccaatgc
    13801 cttcccatcg atggcgatgc gatcaagcat ctccgcgacg gtcctcgttt tgggagtttc
    13861 gaggtctgga ggttcgatga cgacaatccg tccagccgcc gccatggccg acaacgccga
    13921 gcgcaatgca aggcggcgca ctttcttcgg cgtgtcgtga gagtggtcgc ggggcctcgg
    13981 tccgaacgtg atcccgcccc ccacgaagat cggcgccttg ttgctcccgt gacgcgcgcg
    14041 ccccgttccc ttctggcggt agatcttccg cgtgctgccg gacacttcac cgcgtgtctt
    14101 tgtcgcgtgt gtgccgcggc ggcgcgaagc cagctgcgcg accagcactt cgtgaagcac
    14161 ggcgcggtga ggcttgattc cgaacacttg gtccgggagg tcgatcttgc cggtctcttt
    14221 gccctttgca tcgaacagct gcgccgtggg catccgggtc acctcgcctt cttcgcctgc
    14281 tgcttgcgga tgacgataag actgcctcgt ggcccgggga tggctccctt gagcatcagc
    14341 aggttccgtt ccgcgtcgac gcgcatgacc ttgagtccac ggaccgtcac gcgttcgccc
    14401 ccgtgccggc cgggcatgcg ctttccgggc cacacacggg caacgtcaga gattccgatc
    14461 gagcccactg cgcggtgcat gagagacaca ccgtgggtgt cccgctgtcc cttgaagttg
    14521 tgccgcttca tcgcgccgga gaagcctttg ccctttgttg tccccgtgac gtctacgacg
    14581 tcgccgcccc cgaacgtgtc gaccttgatc tcctgaccga ccgtaaagtt ctcgccagat
    14641 gtgcgaaact cacggagcac tttgcgcggg gccgtcttcg ccttagcgaa gacccccttc
    14701 agcggcttat tcacttttcg ctccgcgatc gattcgaagc cgacctgtac ggcagcatac
    14761 ccatcgcgtt caggcgtgcg aacgttggtg accacgcacg ggccggcctc gatgaccgtc
    14821 accgacacaa tgttgcccgc gtcatcgaag atctgagaca taccaacttt tcgtccgacg
    14881 attgctgtca tggctaactc cgacttcgtc tcccccgtct ggtttagagt ttgatctcga
    14941 tatccacccc agccggcaaa tccaaatggc tcaacgcgtc caccgttttt tgcgtcggtt
    15001 ccagaatatc aatgagtcgc ttgtgtgttc gcaactcaaa gtgctccatg gactctttgt
    15061 cgatatgcgg cgagcggatc acgcaaaaga gattccggtc tgtcggcagc ggcaccggcc
    15121 cggatacccg ggcgccggtg cgacgcaccg tctcgacgat ccgctcggcc gactggtcga
    15181 gcgctttatg gtcaaatgct ctgagcttga ttcggatgcg ctgggccatg actacgcggt
    15241 cacctttgtg acgacgccgg cgcccacggt ccgcccacct tcccggatcg caaaccgcag
    15301 cccctcttcc agcgccaccg gcgtgatcaa cgccacgtcc atgttcacgt tgtcccccgg
    15361 catcaccatc tccatcccat cgggcagctt gatgtccccc gtcacgtccg tcgtccggaa
    15421 gtagaactgc ggccggtacc ccgtgaagaa cggcgtgtgc cgccccccct cttccttggt
    15481 cagcacgtac acctccgcct tgaacttcgt gtgcggcttg atgctccccg gtttcgccag
    15541 caccatcccc cgctcgatct cttccttctc cactccccgc agcagcacgc ccacgttgtc
    15601 ccccgccacc gcctcgtcca gcgtcttcct gaacatctcg atccccgtca ccaccgtccg
    15661 cttcgactcc gggcgcagcc ccacgatctc cacctcctcg cccaccttca ccttcccccg
    15721 ctccacccgc cccgtcgcca ccgtcccccg ccccgtgatc gtgaagatgt cctccaccgc
    15781 catcaggaac ggcttgtcca cctcccgctg cggcgtcggg atgtagctgt ccaccgcctc
    15841 caccagctcc cagatcttgt ccacccactc gttctggccc cgcgcgatcc ccttcccctt
    15901 cgccccctcc agcgcttcca gcgccttcag cgccgagccc cgcacgatcg gcgtcttctc
    15961 gcccggaaac tgatacttgg tgagcagatc ccgcacttcc acctccacca gctccagcag
    16021 ctcggggtca tccaccatgt cgatcttgtt caggaagacc gcgatgtacg gtacgttcac
    16081 ctgccgcgcc agcagcacgt gctcccgcgt ctgcggcatc ggcccgtccg ccgcgctcac
    16141 caccagcacc gccccatcca tctgcgccgc ccccgtgatc atgttcttga tgtagtccgc
    16201 gtgccccggc gcgtcgatgt gcgcgtagtg ccgcttcggg ctctcgtact ccacatggct
    16261 gatgttgatc gtcagccccc gcgccttctc ttccggcgcg ttgtcgatgt ccgcgtaccc
    16321 cttggcctgc gtcaccccca cccccgccag cgtgtgggtg atcgccgccg tcagcgtcgt
    16381 cttcccgtgg tccacgtgcc cgatcgtccc gatgttcacg tgcggcttcg tccgctcaaa
    16441 cttctgcttg gccatggcgt ctgctcctcg acaagagtag gtgaccagag actagacgcg
    16501 gcccatgccg ggcactctag tcactggtca ccagacacga gtcattggtc tggagcccac
    16561 gaccggattc gaaccggtga ccttgtcctt accaaggaca cgctctgccg actgagctac
    16621 gtgggcgtat cgcaaaccgt attataggcg tcgctcgctt gcaatggcaa gcactgccga
    16681 cgcaggaggc gcgcctgtag cggcgaatga cgcgccaaga cttcaagtag gagggaggag
    16741 gacacaatgg actggatatg gtggatcctc gtacttgcgc tgatctggtt cctcttcttc
    16801 aagaatccag cataagagcg tcctagtgat atagaaaaag agccccagtg atgctggggc
    16861 tctttcattt cgagcgttag taggatcttg tggaggcggc gcccggattc gaaccgggga
    16921 atgggggttt tgcagacccc tgccttgcct cttggctacg ccgccttaac actctaacga
    16981 cgggataatg ggagaaaggg ataaggggat aactttctcc ctttatccca tatcccctta
    17041 tcccgtagtt tcgtttgatg gagcgggaga cgggacttga acccgcgacc ttctccttgg
    17101 caaggagacg ctctaccact gagctactcc cgcagccgga cctcattata tatggtgccc
    17161 agggtgggag tcgaacccac atgccgcttt cgcggcacga cattttaagt gtcgcgtgta
    17221 aaccattcca ccaccggggc tagactcgtg ctttccgcgg ggaaattcta tcacccggcg
    17281 attgccgcga caacgattag actagctcca gtaagtccgc gtccgattcc ttctcaaaag
    17341 atagagaaag aaaggggccc ccagcccagc cgtgacaatt cccaccggta tttctgttgg
    17401 tgcgagaatc gtccgcgcca ccgtatccgc gagcagcagc gtgatcccac ccgcgagggt
    17461 agcggccggc agcagccgcc ggtggtccgg accggtgatt agacgcac
//
LOCUS       DBNGSB010000144         4760 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3168241, whole genome shotgun sequence.
ACCESSION   DBNGSB010000144 DBNGSB010000000
VERSION     DBNGSB010000144.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3168241"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="AC159B_04140"
     CDS             <1..575
                     /locus_tag="AC159B_04140"
                     /inference="COORDINATES: protein motif:HMM:NF024739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3376968.1"
                     /translation="LVKAEDAPKTLNELLDPKWKGKVVMPDPTVHTTTAVWLSNMQKV
                     LGDQYRPFVERLAAQVSLVESFLPVVQRVIAGEFPLGITYVKYVYIFGSEGAPLDYVR
                     LDPVLAEAHHIAIGAKAPHPNAARLFVDVFTSRLGLLALARAGEFVLVPGVYPPIKDA
                     DKLRVIMMEDLDEQELARFRQSIGALFTKR"
     gene            588..2306
                     /locus_tag="AC159B_04145"
     CDS             588..2306
                     /locus_tag="AC159B_04145"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3376969.1"
                     /translation="MAYVPAVPRQAGWSVRVDPVALFAVGIGLVLAFLALYPTIMLFY
                     GSVSTAPMGLPGQFTLQHYRTAYTDPATYRLIANSFVFAGGSALLSLVLAGALAWITV
                     RTNAPGRGLFEMVAIVPNVLPPLLISTSWVLLLSPRIGLVNVVLAKVGLPPFNVYSMP
                     GMIFVEGLILTPLAYLIVAAAMRSMDPSLEEAARVTGSSTLDAARRVTLPLIRPALLA
                     AGILNFVRALESFDTPAIIALPARIEVITTKIFREALSVSPPNHNLAATYGMSLLAIT
                     MTFVYLYRRFTGRAEMFTTITGRGYRPHTIDLGAWRYSASAIASVVLCLIVVLPFLML
                     ALTSILPFYEVPTWSTLQVLTLKHYDFLLHNSRVGRAFQISLVLATVGATLAMILASA
                     VAFLSVRTKIAGRAILEFLTFIPWAFPGTALAIGLLWGYVRLPIAIYATIWILMIAYI
                     TRFLPYGLRAMTSTMVQVHTELEEASRVCGAGLLTTFRRILLPLLRPGFMAGWAILVT
                     IFMREFSLSLFLYTPRSEPLGPLLYHFWIDAQQGRMAALGVLISLASVALVVAARRIS
                     RTPLAS"
     gene            complement(2303..3400)
                     /locus_tag="AC159B_04150"
     CDS             complement(2303..3400)
                     /locus_tag="AC159B_04150"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3376970.1"
                     /translation="MPAIRLMDLTKFYRVGRQRVAAVEAVTLEAQNGKIITLLGPSGC
                     GKSTTLRCIAGLERPDDGEIFFGDRLIFSRARRVLVPPDRRHVGMVFQSYAIWPHLTV
                     SQNVAYPLEVRGVPSAQIANRVRDVLALVGLSGLEDRPAPFLRGGQQQRVALARALVY
                     EPEVLLLDEPLSNLDAKVREQVREEFRMLQQRLGITALYVTHDQLEALVLSDVIAVMH
                     QGRIMEVGTPQDLYARPRHPFTASFLGEISYLKGTVAASDGSTTSVRTPAGLLRCARM
                     TPIEAGTSVVAGVRPEHVSLGRHRPPPQANAFEGTVVSALYEGTRIKHRIAIGEQTLL
                     AYGPELYATGERLYASIDPARVILLADLAAG"
     gene            complement(3401..4480)
                     /locus_tag="AC159B_04155"
     CDS             complement(3401..4480)
                     /locus_tag="AC159B_04155"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR3376971.1"
                     /translation="MKKKRLRIAVLMGGPSPERAVSMDTGLQIISSLDPQKYNVLPVE
                     ITKEGKWLPRPDLLQLPASTQSRKRANERQEPSPLAKSQDPRPTTYDRFMESEQVDVA
                     FIAMHGPYGEDGTMQGLLELLGIPYTGSRVLASALAMDKLRSRQIFEWHGIPVPAYRS
                     VTVDTWRDRGHVHEQVSRDLGYPCVVKPNAVGSSIGVSIVREPTGLDPAVDKAFTYGP
                     VVLIEEYVSGTELACGIVDDPATGEPVSLPLIEIIPHADFYNYEAKYAAGGSEHIIPA
                     RVSEEIAARAQALAVRAYKALGCEGMSRVDIIARSTDIVVLEVNTIPGMTPTSLLPDA
                     AKAVGISFAELLDRIVENALRQRKR"
BASE COUNT          800 a         1554 c         1460 g          946 t
ORIGIN      
        1 gcctcgtgaa ggccgaagat gccccaaaga ctctgaacga gttgctcgac ccgaagtgga
       61 agggcaaggt cgtgatgccg gaccccaccg tgcacacgac gacagcggtc tggttgtcca
      121 acatgcaaaa ggttctgggt gaccagtacc gcccattcgt ggagcgcctg gcggctcaag
      181 tgtccctggt tgagtccttc ctgcccgtgg tgcagcgcgt gatcgccggc gaatttccgc
      241 tcggcatcac gtacgtgaaa tacgtgtaca tcttcggcag cgaaggcgcg ccgcttgatt
      301 acgtccggct cgacccggtc ctcgcggaag cgcatcacat cgcaatcggc gcaaaggcgc
      361 cccatcccaa cgcggcacgg ttgttcgtgg acgtgttcac gtcgcggctg ggcctgctcg
      421 ccctggcccg agccggcgag tttgtgctcg tgccgggcgt gtatccgccg atcaaggacg
      481 ccgacaaact gcgcgtcatc atgatggagg atctcgacga gcaggagctg gcacggttcc
      541 gccagtcgat aggagctctc tttacgaagc gctgaccgcc tctagccgtg gcctacgtcc
      601 ccgcggtccc gcgtcaggcg ggctggtcgg tccgggtcga tcccgtcgcg ctgtttgccg
      661 tcgggatcgg cctcgtgctt gcattcctcg cgctctatcc caccatcatg ctcttctacg
      721 gcagcgtgag caccgcgccc atgggcctgc cggggcaatt tacgctgcag cactatcgca
      781 ccgcgtacac tgatcccgca acgtaccggc tgatcgccaa ctcgttcgtc tttgcgggtg
      841 gctcggcgct gctgtcactc gtcctggccg gtgcgctcgc gtggatcacc gtgcgcacga
      901 acgcgcccgg ccgggggttg ttcgagatgg tcgcgatcgt gccgaacgta ctgccgccgc
      961 ttctgatctc gacatcgtgg gtcctcctgc tgagcccgcg catcggcctg gtcaacgtcg
     1021 tactcgccaa ggtgggactc ccgccgttca atgtctactc gatgccggga atgatcttcg
     1081 tcgagggact gatcctgacg ccgctcgcgt atctgatcgt cgcggcggcg atgcggtcga
     1141 tggatccgtc gctggaagag gcggcgcgcg taaccggctc ctccacgctg gatgctgcgc
     1201 gccgggtgac gctgccgctg atcaggccgg cgctgctggc cgctgggatt ttgaacttcg
     1261 tgcgcgcgct cgagagtttc gatacgccgg cgatcattgc gctgcccgcg cggatcgaag
     1321 tcatcaccac gaagatcttc cgtgaggccc tctcggtctc tccgcccaac cacaatctcg
     1381 ccgcaaccta cggcatgagc ctgctggcca tcacgatgac cttcgtctat ctctaccgcc
     1441 ggttcacggg ccgcgccgag atgtttacga cgattaccgg acgcggctac cgcccacaca
     1501 cgatcgatct tggggcctgg cggtactccg cctctgccat tgcctcagtc gtgctctgcc
     1561 tgatcgtagt tctgccgttc ttgatgctgg cgctgacgtc gattctcccg ttctacgagg
     1621 tgcctacttg gtcgacgctg caggtgctga cgctgaagca ctatgacttc ctgctgcaca
     1681 acagccgcgt gggaagagcg ttccagatca gcctggtgct ggcaaccgtg ggcgcaacgc
     1741 tggcgatgat cctggcgtcc gcggtcgcgt tcctctcagt gcgcacgaag atcgcggggc
     1801 gtgcgatcct tgagttcctg acgttcatcc cgtgggcgtt ccccgggacg gcccttgcta
     1861 tcgggttgct gtggggctat gtccgcctgc cgatcgcgat ttacgcgacc atctggatcc
     1921 tgatgattgc ctacatcacg cgatttctgc cctacggcct gcgcgcaatg acgagcacga
     1981 tggtccaggt acacacggag ctggaagaag catcccgtgt ttgcggcgcc gggctgctca
     2041 caacattccg tcgtatactc ctgccgctgc tgcgacccgg cttcatggca ggctgggcga
     2101 ttctggtcac gatcttcatg agggagttca gcctatcgtt gtttctctat acaccacgat
     2161 ccgagccgct gggtccgctg ctctaccact tctggatcga cgcgcaacag ggccgcatgg
     2221 ccgcgttggg agtcctcatc agcctggcgt cggtggcgct ggtcgtcgcg gcgcgccgca
     2281 tcagccgcac gccgctcgcc tcttagccgg cagccaaatc cgccagcagg atgacccgtg
     2341 cgggatcaat cgaggcgtac aggcgctcgc cggtcgcgta caattcgggc ccgtaggcca
     2401 ggagcgtctg ctcgccgatc gcgatgcggt gtttgattcg cgtgccttcg tagagggcgg
     2461 acaccactgt tccctcgaac gcatttgcct gcgggggcgg ccggtgacgt ccgagggaca
     2521 cgtgctctgg acggacgccc gcaaccacac tggtccccgc ctcgatcggc gtcatgcgcg
     2581 cacagcgcag cagaccggcc ggggtgcgca cggatgtcgt cgatccatcc gacgctgcga
     2641 ccgttccctt gaggtaactg atctcgccca gaaaactggc ggtaaacgga tgacgcggac
     2701 gggcgtagag atcttgaggc gtgcccactt ccatgatccg tccctgatgc atgaccgcga
     2761 tcacatccga gagcacgagg gcctccagct gatcgtgggt cacgtagagc gcggtgattc
     2821 ccagccgctg ctgcagcatc cggaactctt cgcggacctg ctcgcgtact ttcgcgtcga
     2881 ggttcgacag cggctcgtcc agcaagagga cttcaggctc atacaccagc gcacgggcca
     2941 gcgcgacacg ctgctgctgt ccgcctctca gaaatggcgc cgggcgatct tccagccccg
     3001 acaggcctac cagcgccagg acgtcacgca cccgattcgc gatctgcgct gatggaactc
     3061 ccctgacctc gagcggatac gccacgttct gggaaacggt caggtggggc cagattgcgt
     3121 atgactggaa gaccattcca acgtgccgcc ggtccggcgg gacaaggacg cgacgtgcac
     3181 ggctgaagat caagcggtcg ccaaagaaaa tctctccatc atcgggacgc tcgagtcccg
     3241 caatgcatcg cagggtcgtc gacttgccgc agccgcttgg accgagcagc gtgatgatct
     3301 tgccgttttg agcttcgagc gtcaccgctt ccactgcggc gacccgttgc cggccgacac
     3361 ggtagaattt cgtgagatcc atcaaccgga tcgcaggcac ttatcttttc cgctgtctca
     3421 gcgcattctc gacgatgcga tccagcaact ccgcgaacga gatgccgaca gccttcgcag
     3481 cgtcaggcag gaggctggtg ggagtcatcc cggggatcgt gttgacttcc agcacgacga
     3541 tgtcggtgct gcgcgcgatg atatccacgc gggacatgcc ttcacagccg agcgccttgt
     3601 acgcgcgcac cgcgagtgcc tgagcgcgcg ccgcgatctc ttcggagacg cgagcgggga
     3661 tgatgtgctc gctgccgccg gcggcgtact tcgcctcata gttgtagaaa tcggcgtggg
     3721 ggataatctc gatcagcggg agagaaaccg gttcgcccgt ggcggggtca tcgacaatcc
     3781 cgcacgccag ctcggtcccg gacacatact cctcgatcag gacgacagga ccgtacgtga
     3841 atgctttgtc cacggccgga tcgagccccg tcggctcgcg gacgatggag acgccgatgc
     3901 tgctgccgac ggcattgggt ttcacgacgc agggatatcc caggtcgcgt gagacctgct
     3961 catggacatg gccgcggtcg cgccatgtgt cgaccgtgac gctccggtac gccggcaccg
     4021 ggatcccgtg ccactcaaag atctgccggc tccgcagctt gtccatcgcg agcgcgctcg
     4081 ccaacacacg agatcctgtg tagggaatgc cgagaagctc gaggagtccc tgcatggtgc
     4141 cgtcttcgcc atacggaccg tgcatggcga tgaacgccac gtcgacctgc tcagattcca
     4201 tgaatcggtc gtaggtcgtt ggtcgtgggt cctgggactt tgctaacggg cttggctctt
     4261 gacgctcgtt tgcgcgcttg cgcgattgcg tactggcggg aagttgaagt aagtccggcc
     4321 gcggaagcca cttgccctct ttcgtgatct ctaccgggag tacgttgtac ttctgcgggt
     4381 ccagcgagct gatgatctgg agccccgtat ccattgacac ggcgcgctca ggcgagggtc
     4441 cacccatcag caccgctatt cgcagcctct tcttcttcac ttaacccggc accaagcaaa
     4501 attcccccgg cctcgaccta gctcggctcc agacccaatc aacgtgcaaa atccaccggc
     4561 ctcgcctcaa gctcggcctc ctcaacagcg tcgttggccc actccgtggg cgatactagt
     4621 gatcgtcact gcttcggcgc tggcaaggga accaccgcca gaccgcggaa aggtgcgtgg
     4681 tgcgccaacg cgacatcgag attatcctca tgcggcaact ggcgagctac ctcatcgtac
     4741 cgatctttgt cgtggataag
//
LOCUS       DBNGSB010000145         3469 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_4088885, whole genome shotgun sequence.
ACCESSION   DBNGSB010000145 DBNGSB010000000
VERSION     DBNGSB010000145.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_4088885"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..909
                     /locus_tag="AC159B_04160"
     CDS             61..909
                     /locus_tag="AC159B_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3376972.1"
                     /translation="MTSIRGLVVKTILYGVLVSFAIFYLIPIFVLVNNGLKSFQEVSL
                     SRMWELPQHITGSSFARALDKLAPNFRNSVLLVVPAALIAAVLGSINGYILARWKFFG
                     ADLVFSLLLFGMFIPYQSVLIPLVQFLQWAGRLFDIRLYGSISGLVLVHIVYGIPIMT
                     LIFRNYYATIPGELVEAARIDGGGILGIYRDVMLPLSMPGFVVAIIWEVTQVWNEFLF
                     AVVITSNPAQQPIQVALQNLAGSQIVEWNVQMAGALLAALPTLLVYIFLGRYFLRGLL
                     AGALKG"
     gene            906..2084
                     /gene="alr"
                     /locus_tag="AC159B_04165"
     CDS             906..2084
                     /gene="alr"
                     /locus_tag="AC159B_04165"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR3376973.1"
                     /translation="MRKDASAQTRKRANALTRRTWAEIDLRALAHNLREIRKLLRPSF
                     QIMAVVKADAYGHGAVEVAEAAVRAGASWLGVATLEEGLALRRAGIRAYVLVLGPTAA
                     SELPAVARHKIGVTIVSPEFLEALLRLGRRPRASLHLKIDTGMTRLGVMVDEADRAIV
                     SLNESAITLEGCYTHLSTPDEPDRKYTEDQLARFAPIVATARRAFPKILIHAGGSAAA
                     IAYPGSHYDLVRIGIALYGVSPAQHLRNRIDLRPVMRLKSRVVRVSRVSEGTAVGYGA
                     TFRAKARTTIATVACGYADGYPRLAGAQGEVIIREMRYPIVGRVSMDYLTVDVANNEV
                     SANDEVELFGEGIGVDEVASKAQTISYEVLTGIGSRVPRIYVDGPGDQEPGTRRSAKE
                     "
     gene            2164..2985
                     /gene="pheA"
                     /locus_tag="AC159B_04170"
     CDS             2164..2985
                     /gene="pheA"
                     /locus_tag="AC159B_04170"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HIR3376974.1"
                     /translation="MKVAFQGERGAYSEEAAEGLYGEVEVMPCSTLREVFETVSVGRA
                     DRGVVPVENSQAGSINETYDLLLAHTLFIVGELDLRIRHYLLALPGQTLESIRQVYSH
                     PQALAQCEEFLRKHNFEPMPASDTAGSARIVASQNLHGVGAIAGHRAGEIYHLSILAE
                     GIETNPFNYTKFLGLSRTTAPRRSDSKTSVVFTTKNVPGALHRAMGAFASRSINLTKL
                     ESRPRREVPWEYVFYADFDGHQDDSESAAALADLRDVTTFLRVLGSYPRSTTPLV"
     gene            2982..>3469
                     /locus_tag="AC159B_04175"
     CDS             2982..>3469
                     /locus_tag="AC159B_04175"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014712.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit B family protein"
                     /protein_id="HIR3376975.1"
                     /translation="MREVVVKPFGDRAILVEFDDELSPLANGRARALARAMRDMHGVR
                     EAIPALRSSLIVIDPLDANRDAIVAAAADLASKVTPEPAYTGRDLEVPVVYGGDGGPD
                     LDEVAETLGFSARELIAVHTQPVYTVYMLGFTQGFPYLGLLPEALRVSRKATPRTREP
                     AGS"
BASE COUNT          650 a         1032 c         1061 g          726 t
ORIGIN      
        1 ggtcgccacc gtcgtgatcc cgtacctggt ctggagcgcc cggacggttc aggaatcctg
       61 atgaccagca tccgggggct cgtggtgaag accatcctct atggcgttct tgtcagtttt
      121 gcgatctttt atctgattcc gatatttgtg ctcgtgaaca acgggttgaa gtcgttccag
      181 gaagtgagcc tgagccggat gtgggaactc ccgcagcaca ttacgggaag cagttttgcg
      241 agggcgctgg ataagctcgc gccgaacttc cgtaacagtg tcctgctggt cgtccccgcg
      301 gcgctgatcg ccgcagtcct gggctcgatc aatggctaca tcctggccag atggaagttt
      361 ttcggggccg acctcgtctt ctcgctgctg ttgttcggga tgttcatccc ctaccagagc
      421 gtgctgatcc cgctggtaca gttccttcag tgggcgggga ggctgttcga catccgtctc
      481 tatggatcga tctccgggct ggtccttgtg catatcgtct atgggatccc catcatgacg
      541 ctgatcttcc gcaactacta tgcgacgatt cccggcgagt tggtggaggc tgcgaggatt
      601 gacggcggtg gcatcctcgg aatttaccgc gatgtcatgc tgccgctctc catgccaggg
      661 ttcgtcgtgg ctattatctg ggaagtgacg caggtctgga acgaatttct gtttgccgtg
      721 gtgatcacga gcaacccggc gcagcagccg atccaggttg ccctccagaa tctggcaggc
      781 agccagattg tcgagtggaa cgtgcagatg gccggcgcac tccttgccgc cctaccgacg
      841 ctgctcgtct acattttcct cggtcggtac ttcttgcgcg ggttgctcgc aggagcgctc
      901 aaaggatgag aaaagacgca agcgcgcaaa cgcgcaagcg cgcaaacgca ctcacacggc
      961 gaacgtgggc ggagatcgat ctccgagcgc ttgcgcataa cttgcgggag atccgaaagc
     1021 tgctacggcc ctcatttcag atcatggcag ttgtgaaagc tgacgcctat ggccacgggg
     1081 cggtcgaagt cgcggaggcc gccgttcgcg cgggcgcatc ctggctgggc gtcgcaacgc
     1141 tggaggaggg attggcgctg cgccgggcgg gaatcagggc ctacgttctc gtccttgggc
     1201 caaccgcagc gagtgaacta cccgctgtcg caaggcacaa gatcggcgtc acgatcgtct
     1261 cccccgagtt cctcgaggcc ttgcttcggc tcggtcggcg tccgagagcc tcgcttcacc
     1321 tcaagatcga tactggtatg acccgcttgg gcgttatggt cgacgaagcg gatcgagcca
     1381 ttgtcagttt gaacgagtcc gcgatcactt tggagggctg ctacacgcac ctgtccaccc
     1441 ccgatgagcc cgatcggaaa tacaccgagg accagctcgc gcgtttcgct ccgatcgttg
     1501 cgaccgcgcg tcgcgccttt ccgaaaatac tgattcatgc aggcggcagc gcagccgcga
     1561 tcgcatatcc aggatcccac tacgatttgg ttcgaatcgg gatcgcgttg tacggagttt
     1621 ctcccgcaca gcacttacga aatagaattg accttcgtcc agtcatgagg ttaaaaagcc
     1681 gcgtcgtccg ggtcagtcgc gtgtcagagg gcacggccgt cggctacggc gcgaccttcc
     1741 gcgcgaaggc gcgaacgacc atcgcgaccg tcgcgtgcgg ctatgcggat ggctatcctc
     1801 ggctcgcggg tgcgcaagga gaagtaatca ttcgtgagat gcgctacccc atcgtggggc
     1861 gcgtgagcat ggattacctg acagtcgacg tagcgaataa cgaggtttca gcaaacgacg
     1921 aagtcgagct ctttggagag gggatcggcg tggacgaggt cgcctcgaag gcgcagacga
     1981 tctcgtacga ggttctgacc ggtattggat cccgcgttcc gcggatctat gttgacggac
     2041 caggggacca ggaaccgggg acccgaagat cggctaagga gtgacttatt acgggtcccc
     2101 ttgtccctgg tcccctggcc cgtcgttcgg tgattcaatg aaggtcgcgc gtcacgggtc
     2161 gcgatgaaag tcgcttttca aggggaacgc ggagcctaca gtgaggaagc cgcagagggg
     2221 ctctacggcg aggtcgaggt catgccatgc tccaccctcc gcgaagtttt cgagacagtc
     2281 tcagtaggtc gggccgaccg cggcgtggtg ccggtggaga actcgcaagc cggatcgatc
     2341 aacgaaacgt atgacctgct gctcgcccac acactgttca ttgtcggcga gctcgacctg
     2401 cgaatccgtc actacctgct tgccctgcct ggccagacgc tggaatcgat tcggcaggtc
     2461 tactcccacc cgcaggcgct cgcgcaatgc gaggagttcc tgcggaagca caattttgaa
     2521 ccaatgcctg ccagcgatac cgcgggcagc gccaggatcg tcgcctcgca gaacctgcac
     2581 ggagtcgggg caatcgccgg gcaccgtgcc ggggagatct atcatctctc gatccttgcc
     2641 gaggggatcg agacgaaccc gttcaactac accaagtttc taggactcag caggaccacg
     2701 gctcctcgtc gttccgattc caagacttcg gttgtcttta ccaccaaaaa tgttcccggc
     2761 gcattacacc gggccatggg tgcattcgca tctcgatcga tcaacctgac caaactggaa
     2821 tcccgtcccc ggcgtgaagt tccctgggag tatgtgttct atgccgattt cgatggccac
     2881 caggatgatt cggagtccgc cgccgcactc gccgatctca gagacgtcac cacatttctc
     2941 cgcgtgctcg gttcctatcc taggtcgacg acgcccctcg tatgagggag gttgttgtca
     3001 agccgttcgg tgaccgcgcc atcctcgtcg agtttgacga tgagctgtct ccgctcgcca
     3061 acggaagggc ccgggctctg gcacgtgcga tgcgtgacat gcacggcgtt cgagaggcga
     3121 taccggctct ccgatcttct ctcatcgtga tcgacccact ggacgctaac cgcgacgcca
     3181 ttgttgccgc cgcggcagat ctcgcgagta aggtaactcc cgagccggcg tacacaggtc
     3241 gtgacctgga ggtcccggtt gtctatggcg gcgatggcgg accggacctt gacgaggtgg
     3301 cggaaacact cggtttcagc gcgcgagagt taatcgcggt tcacactcag cccgtataca
     3361 cggtctatat gctggggttt acacagggat ttccgtatct cggattgcta cccgaagcgc
     3421 tgcgtgtctc gaggaaagcc acgccccgca ctcgagagcc ggcaggatc
//
LOCUS       DBNGSB010000146        10022 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_256054, whole genome shotgun sequence.
ACCESSION   DBNGSB010000146 DBNGSB010000000
VERSION     DBNGSB010000146.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 10022)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10022)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10022
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_256054"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="AC159B_04180"
     CDS             complement(<1..404)
                     /locus_tag="AC159B_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3376976.1"
                     /translation="MIKAISAVARREITIFLRYPTWVIGALVWPALFPLMYLFASRAL
                     AGPQGEGMETFARVAGTANYAGFIVVGTTAWMWLNVTLWSVGTSLRNEQLRGTLESNW
                     LTSSPRFLVMLGIAAAHALNFMVFLLVGVLEFV"
     gene            complement(397..1422)
                     /locus_tag="AC159B_04185"
     CDS             complement(397..1422)
                     /locus_tag="AC159B_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3376977.1"
                     /translation="MSTAPVHAVELEGLTKRFTARGIDENRGRRRAVRREITAVDHVD
                     LQIRRGELFGLLGPNGAGKTTTIRMICTLLEPTSGRVRVWNHDASTVPASVRRSLGVL
                     LMGERSVYWKLSARENLEYFAALYEVPAATAKARISELLDRVGLTARADDLVERYSTG
                     MRQRLALIKSMVHDPPVLLLDEPTTGLDPQAARNIRDLIRHLHSSEGKTIILTTHYME
                     ESDQLCERIGIIDYGKIIALDAPQTLKRVHGERPVLRLDLEDITDGVEPALRSIPGVT
                     RVVVQPKGTNAGGYEAIVHLSDLRETLPRAVETVSRAGGRVRHLQLVETTLEDVFIAL
                     TGRTLRD"
     gene            complement(1419..1763)
                     /locus_tag="AC159B_04190"
     CDS             complement(1419..1763)
                     /locus_tag="AC159B_04190"
                     /inference="COORDINATES: protein motif:HMM:NF046909.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT-like domain-containing protein"
                     /protein_id="HIR3376978.1"
                     /translation="MNDRLRILEMVKEGKVTPEEGERLLEELNRAPRGPAPILRVRIS
                     SPGGPKVQFTIPVSVAGSVLALIPETARKKLETRGIDLDQLIEAIRTGDAIGQIVDIK
                     EPGGTSVEVTVE"
     gene            complement(1760..1996)
                     /locus_tag="AC159B_04195"
     CDS             complement(1760..1996)
                     /locus_tag="AC159B_04195"
                     /inference="COORDINATES: protein motif:HMM:NF046909.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT-like domain-containing protein"
                     /protein_id="HIR3376979.1"
                     /translation="MDEERQMILRMLKEGKISVEEANALLRALEKEDGETAPSEPDDE
                     TRAGGSHSFAEFGAELASVIRDIVDTIPKDVLKP"
     gene            complement(2021..2398)
                     /locus_tag="AC159B_04200"
     CDS             complement(2021..2398)
                     /locus_tag="AC159B_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 family protein"
                     /protein_id="HIR3376980.1"
                     /translation="MPQTPGKCPVCGHDLEVVRLQCTSCGTSVEGHFELSRFSRLEPE
                     QLAFLELFLKARGNLKDVERELGLSYPTVRNRLEAMLAALGFAAEPEGKTEAVRRRRE
                     ILDSLDAGKISAEEALRLLRDRK"
     gene            complement(2658..3365)
                     /locus_tag="AC159B_04205"
     CDS             complement(2658..3365)
                     /locus_tag="AC159B_04205"
                     /inference="COORDINATES: protein motif:HMM:NF013222.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HIR3376981.1"
                     /translation="MRVANTVDLKNKTNELLRHAIAGDPVIITLRGKPAASLTRLTEA
                     ELESFVLRHAGIRRPETRPQLPWRSTSLRTPLGTAYVAYSEQGVGHLDLADSEASFER
                     TFRRRFSKPVHRDVRPPGNLRRALIGFFTQQEPFRGDVDLTLVGPFERSVLEQLRRIP
                     RGQVRTYREVATALGHPSASRAVGNACAKNPVPLIIPCHRVIRSDGGLGGYSLRGGPG
                     LKRQLLNEEGANLEKRK"
     gene            complement(3470..5530)
                     /locus_tag="AC159B_04210"
     CDS             complement(3470..5530)
                     /locus_tag="AC159B_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR3376982.1"
                     /translation="MDCADCSLHVQRAIAALPGVESVAVSLATEKAVVRLDLGQVGMD
                     AIHKAVRGSGYEVVASNTPPSSTPPRTSPRGLLVLGVIAAAVLLIVAAGEWVGWIDEI
                     NASLPWPVALVLIVIGGFPVFRNVIRAALHRQVLAHTLMTVGVVAAAAVGQWASAVVV
                     VFFMRIGDYIERFTTERATRAVKDLTELAPQMARVEQDGTEREIPIGQVRVGDVVVVR
                     PGEVIPVDGQVIDGQATVDQASITGESMPVEVGPGAAVFAATWARLGSIRVRTEHVGE
                     DSTFGRIIKLVGEAEIHRAKVQRVADRFAGYYLPVVAAIAALTFLVRRDPLATAAVLV
                     VACSCSFALATPIAMVASMGAAAKRGLIIKGGKYLEALARADVLLVDKTGTLTLGRLV
                     LTDVVPLDGLSAESALALAASAERYSEHPVAEAVRQAAATRNIASEAPRRFEAVPGLG
                     VRAQVNGKTVTVGNRRLLPAEAEHPVVKKLEAEGKTPILVAEDDRLIAVLAVADESRA
                     EVPAALMAVRALGVRHIELLTGDTERTASALASELGIAYRAELMPEDKIEVVKAFQRE
                     GRTVVMIGDGVNDAPALAQADVGIAMGAAGSDVAIEAAHIALMREDWWLVPAAFRIAR
                     RTLGVVKINIGFTTVFNAVGLSLAATGILPPMLAAALQSIPDAGILVNSSRLLRPGRL
                     HPPA"
     gene            complement(5644..5964)
                     /locus_tag="AC159B_04215"
     CDS             complement(5644..5964)
                     /locus_tag="AC159B_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376983.1"
                     /translation="MRQYLTRHVGLSIALGAAWLLIAFLVPQTRAYVIFWGPLVAFSI
                     ALRPAALLLGDIASCASFDRWSCPPLVDITMWILAGTVVLFVLYLMGMLTFSLVRAIR
                     SVTT"
     gene            complement(6025..7905)
                     /gene="uvrC"
                     /locus_tag="AC159B_04220"
     CDS             complement(6025..7905)
                     /gene="uvrC"
                     /locus_tag="AC159B_04220"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HIR3376984.1"
                     /translation="MAIANLEEKLRLVPARPGVYLMKDAAGRVIYVGKAASLRNRVRQ
                     YFQDSSHLESPRIRYLMSKIADFDVVACENEVEALLLEVNLIKRHQPSYNVRMADDKA
                     FPYVKITNEAFPKISMTRRVLRDGGKYFGPYPYHEPKLVRRTIRTIRKLFKIRSCNIE
                     ITRDLPRPCLDYYIGLCTAPCVSWGANPEQYIDQVRQATLFLEGKEESLLDELRKEMD
                     RAAEAMEFERAAQLRDQIQAMEAIYEKQRAASVGLEDRDVMAIAQAGDLACVQILFVR
                     AGRLQGQEHFVLENTRGVNRAEALGEFVKQYYQDAPGVPREVVLQEAIEDMPVIAEWL
                     SQKRGTKVEVIVPQRGARERLVEMAAENAALFLQHERARRSGLEGAAIKELQEILNLE
                     NPPFRIEAYDVSNFHAGETVGSMVVFEGGRPLKRAYRRFKMTWADGPNDYAMFREMLQ
                     RRFALGREEQEKLDREEPVRVKWSVLPDLVLIDGGRGQLNAALDVLFEYNHMIPAVGL
                     AKKEEMVVTVADPEPVALPRDSHALQMLQRIRDEAHRFANTYHQKLRQRKIVFSALDE
                     IQGIGEKRKRDLIRHFGSVRQIRQANVEQIAGVIGPKMAQKVSEYLQQHPDVRYKDEA
                     VR"
     gene            complement(8037..9149)
                     /locus_tag="AC159B_04225"
     CDS             complement(8037..9149)
                     /locus_tag="AC159B_04225"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR3376985.1"
                     /translation="MKIERFEMERFQSTWEHTVEFNLADSGVLPLTLSGLVDHSWVQE
                     VLAHEPIGYGMTNGSLELRTEIAGLYHAAGPEHVLVTSGSAEANFLITWALLQPGDEA
                     VVMLPNYMQIPLLIRGLGAEAKTWWLTEANQWGPDLDDLARLVTPRTKAIFICNPNNP
                     TGAVLTVEAMNTICELAAKVNAWVVADEVYRGGERDGHTSPSFRGRYARALVTGGLSK
                     VYGLPGLRIGWILGPHEMVDSLWGYHDYTTISPNLIGDRLARMALTPRTRQRLSLRAR
                     SILNENFLVLSRWVDDLGSVVTWAPPQAGAIAFIRYRPKTNSTELATRLRKEFDVLVV
                     PGDHFQMDGFIRVGFGGERRLLEEGLRRTGDLLRSL"
     gene            complement(9196..>10022)
                     /locus_tag="AC159B_04230"
     CDS             complement(9196..>10022)
                     /locus_tag="AC159B_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR3376986.1"
                     /translation="PIGRTPRSNPATYTKTFDLVRDLFAQTPDARMRGYTPGRFSFNV
                     KGGRCEACEGDGIVRIEMHFLPDVYVPCEVCKGKRYNRETLEVHYKGKSISDVLEMTV
                     AEAVQFFENVPRIRRKLQTLEDVGLGYVKLGQPATTLSGGEAQRVKLSAELSRRDTGQ
                     TVYILDEPTVGLHFADVERLLSVLHRLVDAGNTVIVIEHNLDVIKTADWIIDLGPEGG
                     ELGGQVIAEGTPEDVARAPDSYTGQFLRRVLKQQAELVLTGNGGRRRNGRANGRRR"
BASE COUNT         1838 a         3228 c         3021 g         1935 t
ORIGIN      
        1 acaaactcca gcacgccgac cagcaggaac accatgaagt tcagcgcatg agcggccgcg
       61 atacccagca tcacgaggaa gcgaggcgac gaggtgagcc agttcgattc cagcgtcccc
      121 cggagctgct cgttgcgcag cgaggtccca acactccaca gggtgacgtt gagccacatc
      181 cacgcggtgg tgccgacgac gatgaatccc gcgtagtttg cggtcccggc aacgcgcgca
      241 aacgtctcca tgccttcgcc ctgtggccca gcgagcgccc ggctggcgaa cagatacatc
      301 agcgggaaca gggcgggcca gactaacgcc ccgatgaccc aggtggggta ccgcaagaag
      361 atggtaattt cccgccgagc gacggccgag atcgctttaa tcacgcaacg tcctaccagt
      421 gagggcgata aagacatctt caagcgtggt ctcgacaagc tgaaggtggc ggacgcgccc
      481 gcccgcgcgg ctcacggttt ccaccgctct cggcagcgtt tcgcgcaggt ctgagagatg
      541 aacaatggcc tcgtagccgc cggcattcgt gcccttcggt tgcacgacga cgcgggtcac
      601 tccgggaatc gagcgaagcg ccggctcgac gccgtccgtg atgtcctcga ggtcaagccg
      661 gagcaccggg cgctccccgt gcacacgctt caacgtctgc ggagcatcca gcgcgatgat
      721 ctttccgtag tcgatgatcc ctatcctctc gcagagctga tcagattcct ccatgtaatg
      781 ggtcgtcagg atgatcgtct ttccctcgct gctgtgcagg tggcgaatga gatcgcgaat
      841 attgcgcgcg gcctgcgggt ccagacccgt cgtgggttcg tccagaagga gcaccggcgg
      901 atcgtggacc atcgatttga taagggccag acgctgccgc atgccggtgc tgtatcgctc
      961 cacgagatcg tcggcacgag cggtcagccc gacgcggtcc agcaactctg agattcgcgc
     1021 cttcgctgtg gcggccggaa cctcatagag cgccgcgaag tattcgagat tttcacgggc
     1081 cgacagcttc cagtacaccg acctctctcc catcaagaga accccaagac tccggcggac
     1141 cgatgcgggc accgtcgaag cgtcatggtt ccatacgcga actcgaccgg acgtgggctc
     1201 gagcagcgtg cagatcatcc tgatcgtggt cgtttttcct gcgccgttgg gtccgagtaa
     1261 tccgaacagc tctccccgcc ggatctgcag gtcgacgtgg tcgacagcag tgatttcgcg
     1321 ccgcaccgcg cggcgccggc ctctattctc gtcgatcccc cgggcggtga agcgtttggt
     1381 caggccctcg agctcaacgg catgcacggg cgctgtgctc actcgacggt gacctccacg
     1441 gacgtccccc ccggctcttt gatatcgacg atctgaccga tggcatcgcc ggtgcggatg
     1501 gcttcgatca gttgatcgag gtcaatgcct ctcgtctcca gctttttccg agcggtttct
     1561 ggaatgaggg cgagcactga accggcgacg ctgaccggaa tagtgaactg caccttcggt
     1621 cccccggggc tcgaaatgcg cactcgcagg atcggtgccg gcccgcgcgg cgcgcggttg
     1681 agttcctcca gcaacctttc tccttcttcc ggggtgacct ttccctcttt caccatctcg
     1741 agaatccgca gccggtcgtt catggcttga ggacgtcttt aggaatggtg tcgacgatat
     1801 cgcggatcac gctcgctaac tccgcgccaa actccgcgaa gctatggctg ccacccgccc
     1861 gggtctcatc atccggctcg gacggagcgg tctctccatc ttctttttcg agcgcgcgca
     1921 gcagagcgtt cgcctcctcg acggagatct tgccttcctt gagcatgcgc agaatcatct
     1981 ggcgctcttc gtccattgcc gcccccgtca attcccgacc ttatttccga tcgcggagca
     2041 accgcaacgc ttcttcggcg ctgatcttac cggcatccaa cgaatcaagg atctcccgcc
     2101 tgcgccgcac cgcttcagtc tttccttctg gctctgcggc aaacccgagt gctgcgagca
     2161 ttgcctcgag ccggttacga acggtgggat acgacaagcc gagttctcgt tcaacgtctt
     2221 tcaggttgcc gcgcgccttc aaaaacagct cgagaaacgc gagttgctcg ggctcaagcc
     2281 gactgaatct ggagagttcg aagtgccctt cgaccgacgt gccgcacgac gtgcactgga
     2341 gacggacgac ttcgagatca tgtccacaaa ccgggcactt cccgggcgtc tggggcattg
     2401 ggactcatcg acctccggca acgtgcgtca cgcgcgggcc ggcttgcgat cccctggctt
     2461 ccctgcgaaa gcccacgaga gcatcttctt ccagattgac ggagaggggt cagccatcat
     2521 ttcgaggtaa actggatttt gcatttaagc caccacctga taaggtttac aatatatttc
     2581 gcaaataagt taaggtataa gttgtttttt gtcaagtgga ggctatcgaa tgttaaataa
     2641 agcgaacaca tgtacggtta cttgcgcttt tccagatttg ccccttcttc atttagaagt
     2701 tggcgcttga gtcctggccc gccacgaagc gagtagcctc caaggccccc atcgctacga
     2761 atgacacggt ggcacggaat gatgagcggg acggggtttt tcgcgcacgc gttacccacg
     2821 gcgcggctgg ccgaggggtg ccccaaggcc gtcgcaactt ccctgtaggt gcggacctgt
     2881 cctcggggaa tccggcgcag ttgctctaag acacttcgct cgaaggggcc caccagcgtg
     2941 agatcgacat cgccacggaa cggctcctgc tgagtgaaaa atccgatgag cgccctccgg
     3001 aggttcccag gtggtcgcac atcgcggtgg acgggtttcg agaaccgccg gcggaaggtg
     3061 cgttcgaacg aggcctcaga gtcagcgaga tccagatgcc ccacgccttg ttccgagtac
     3121 gccacgtacg cggtcccgag gggtgtgcgt agcgacgtgg agcgccaggg taattgcggg
     3181 cgtgtctcgg gccggcgaat gccggcgtga cggaggacga acgattctag ctccgcctcg
     3241 gtcaagcgag tcagtgacgc cgccggttta ccgcggagcg tgatgatgac cggatcacct
     3301 gcgatcgcat gccgcagaag ctcgtttgtc ttgttcttta aatcgacggt gttggcgacc
     3361 ctcatggcta tactcccccg tggtggcttc ttttctacat agccattaaa atagctatgt
     3421 gtttttcatc ctaccaggca cgagggggga cgtcaacagg aaggcgccgc taggcgggag
     3481 ggtggagccg gccgggacgg agcaggcggg aggagttcac gaggatcccc gcgtcgggaa
     3541 tcgactggag tgcagcggcg agcattggag gaaggatacc cgtcgccgcc agcgagaggc
     3601 cgaccgcgtt gaatacggtt gtgaagccga tattaatttt cacgacccct aacgtccggc
     3661 gagcgatgcg gaatgcggcc gggaccagcc accaatcctc ccgcatgagg gcgatgtggg
     3721 ctgcttcgat cgcgacgtcg ctgcccgctg cccccatcgc gatccccacg tcggcctgcg
     3781 cgagggcggg cgcatcattc acgccgtcgc cgatcatcac aactgtacgc ccctcccgtt
     3841 ggaacgcctt cacgacctcg atcttgtcct cgggcatcag ttcagcgcga taggcgattc
     3901 cgagttcact ggccagcgcc gacgccgtgc gttcggtatc acctgttaac agctcgatgt
     3961 gccgtacacc aagagcgcgc acggccatca acgcggccgg cacttctgca cgcgattcat
     4021 cggcgacggc gagaactgcg ataagccggt catcctctgc gacgagaatg ggggtcttcc
     4081 cctccgcttc gagcttcttt accactgggt gttctgcttc tgccggaagc agccgcctat
     4141 tgccaacggt caccgtcttg ccgttcacct gtgcgcgcac gccgaggccg ggaaccgctt
     4201 cgaatctccg gggcgcctcg ctcgcgatgt tccgggtggc cgctgcctgt cggaccgctt
     4261 cggcgactgg atgctcggag tagcgttccg cagaggccgc gagcgccagc gcgctctccg
     4321 cggaaaggcc gtccaagggg accacgtcgg tgagcactaa acggccaagc gtgagcgtgc
     4381 cagttttgtc cacgagtaga acgtctgccc tggcgagggc ctcaagatac ttgccgcctt
     4441 taataatgag tccgcgtttc gccgccgcac ccatcgatgc gaccatcgca atcggcgtgg
     4501 cgagggcaaa ggagcatgag caggcgacga cgagcaccgc cgccgtcgcc agcggatccc
     4561 ggcgcacaag aaacgtcagt gccgcgattg cggcaaccac aggcaagtaa tatccagcga
     4621 aacggtccgc gacgcgctgc accttggccc ggtgaatctc tgcctctccc accagtttga
     4681 taatgcgccc gaacgtcgag tcttcaccca cgtgctcggt ccggacgcgg atgctgccga
     4741 gccttgccca cgtcgcggcg aagacggctg ctccagggcc gacctccacc ggcatcgact
     4801 ctccggtgat gctcgcttga tccacggtgg cctggccgtc aatgacttgt ccgtctaccg
     4861 gaatgacttc accgggacga acgaccacaa cgtcgccgac gcggacctgc ccaatgggaa
     4921 tctcgcgctc ggtgccatcc tgctccaccc gcgccatctg cggcgcgagt tcagtgaggt
     4981 ctttgaccgc acgcgtcgct cgctcagttg tgaaccgctc gatgtagtct ccgatgcgca
     5041 tgaagaaaac gacgacgacg gcgctcgccc actgtcccac cgccgccgcg gcgaccacgc
     5101 cgaccgtcat cagcgtgtgc gccagcacct gcctgtggag agccgcgcga atgacgttgc
     5161 gaaacaccgg gaagccgccg atgacgatca ggacgagcgc aacgggccac gggagacttg
     5221 cattgatctc gtcgatccag ccgacccact ctccggcggc gacgatcagc agcactgcgg
     5281 ccgcgatgac tccgaggacc agcaatcctc tcggggatgt tcggggcggc gtgctagagg
     5341 gtggcgtatt cgacgccacc acttcgtacc ccgacccccg gaccgccttg tggatggcgt
     5401 ccatgccaac ctggccaaga tcgagacgga ccaccgcctt ctcggtagcg agcgaaacgg
     5461 ccacggactc gacgccggga agggcggcaa tggcgcgctg gacgtggagc gagcagtcgg
     5521 cgcagtccat gccgcggatg gggacttcga ccgtacgaat tgccacgatt cttatcttac
     5581 atgacgggcg gcctggcccc aggggcctgg caccttgggg tcaggcccct tagcctggat
     5641 gggtcaagtt gttactgagc gaatggcgcg aacgaggctg aacgtgagca ttcccatcag
     5701 ataaagcacg aacaacacga cggttcccgc cagaatccac atcgtgatgt cgaccagcgg
     5761 cgggcacgac cagcgatcaa aactcgcaca gctggcgata tctcccagca gaagcgcggc
     5821 gggtcgcagc gcgatggaga aggcaacgag aggaccccag aagatgacgt atgccctagt
     5881 ttgggggaca agaaaggcaa tcaggagcca ggcagcgcca agggcgatgc tcaagccgac
     5941 gtgccgggtc agatactggc gcatggttct caacctccag atattccatg ccctctccac
     6001 ccggcgtcga tacctacgag gttttcagcg caccgcctcg tccttgtagc gaacgtctgg
     6061 atgctgctgc agatactcag acactttttg cgccattttt ggcccgatca ctccggcaat
     6121 ctgctccacg ttcgcctgac gaatttgtct gaccgacccg aagtgacgga tgagatcgcg
     6181 cttgcgtttc tccccgatgc cctggatttc atcgagcgcc gaaaacacga ttttccgctg
     6241 gcgcagcttc tgatgatagg tattcgcaaa tcggtgcgcc tcgtcgcgga ttcgttgcag
     6301 catctgcagc gcgtgtgagt ccctggggag agcgaccggt tccggatcag cgacggtgac
     6361 gaccatctct tccttcttcg ccagacccac cgccgggatc atgtgattgt actcaaacag
     6421 cacgtcgagc gccgcgttga gttgcccccg cccaccgtcg atcaacacga ggtccgggag
     6481 caccgaccac ttcacccgca ccggctcctc ccggtcgagc ttctcctgct cttcgcgccc
     6541 cagcgcaaac cgccgctgga gcatctcccg gaacatcgcg tagtcgttgg gcccgtcggc
     6601 ccacgtcatc ttgaaccgcc ggtaggctcg tttgagcggc cggccgccct cgaacaccac
     6661 catcgagccc accgtctcgc cggcgtggaa gttgctgacg tcgtaggcct cgatgcggaa
     6721 aggtgggttc tccaggttca agatttcctg cagctctttg atggcggcgc cctcaagacc
     6781 ggaccgccgc gcgcgttcat gctgcaggaa gagcgcggca ttctccgctg ccatctccac
     6841 caggcgttcc cgggcacccc gctgcggaac gatgacttcc actttggtgc cgcgtttctg
     6901 cgagagccac tcggcgatga cgggcatgtc ctcgatggcc tcctgcagca cgacctctcg
     6961 cgggacaccg ggcgcgtcct gatagtattg cttgacgaat tcgccaagcg cttcggcccg
     7021 gttgacgccc cgcgtgttct cgagcacgaa gtgttcctga ccctgcagcc tcccggcgcg
     7081 cacgaacaga atctgcacac atgccagatc cccggcctgg gcgatcgcca tgacgtcgcg
     7141 gtcctcgaga ccgacactgg cggcccgctg cttctcgtag atcgcttcca tggcctggat
     7201 ctggtctcgc agctgcgcgg cgcgctcgaa ttccatggct tccgctgcgc ggtccatctc
     7261 cttgcgcagt tcatcaagga gcgactcttc cttgccttcg aggaacagcg tggcctgccg
     7321 gacctgatca atgtattgct cgggattggc gccccaggag acacacggcg cagtacacag
     7381 accgatgtaa tagtcgagac acggccgcgg caggtcccgt gtaatttcga tgttgcagga
     7441 tcggatcttg aagagcttgc ggatagtgcg aatcgtccgg cgcaccagtt tcggctcatg
     7501 gtagggatac gggccgaagt acttcccacc gtcgcgcagc acgcgccgcg tcatcgagat
     7561 ctttgggaaa gcttcgttcg tgattttcac gtacgggaac gctttgtcgt ccgccatacg
     7621 gacgttgtag gagggctggt ggcgcttgat gaggttgact tcgagcaaca gcgcctccac
     7681 ctcgttctcg caggccacga cgtcgaaatc tgcgatcttg gacatgagat accggatgcg
     7741 cgggctttcg aggtgggagc tgtcctgaaa atactgacgc acgcggttgc gcagggaggc
     7801 ggctttgccg acatagatca cccggccggc ggcatccttc attagataca cgcccggccg
     7861 agccggcact agccgaagtt tttcttcaag gttcgcaata gccattcatg tgccggcggt
     7921 ctcggaggcc ttctaagtct cttggtcttc ttggtctacc gaacgagaag cgggtcccag
     7981 tgggctagaa gacctaggag actagaagac tcccggcgac cgcagatacg aaaatattat
     8041 agcgaccgaa ggaggtctcc ggtccggcgg agaccttctt ccaggaggcg gcgctcgccg
     8101 ccgaagccga cgcggatgaa cccgtccatc tggaaatgat cgccggggac cacgagcacg
     8161 tcgaattcct tccgcaaccg cgtcgccaat tccgtagagt tcgtcttcgg gcggtaccga
     8221 atgaaggcaa ttgcgccggc ctgtggggga gcccaggtca ccacgctccc gagatcgtca
     8281 acccaccggc tcaggacgag aaagttttcg ttgagaatcg accgcgctct gagggacagt
     8341 cgctgtcgcg tgcgcggcgt gagcgccatc cgcgccagcc ggtccccgat gaggttcggc
     8401 gagatcgtcg tgtaatcgtg gtagccccac agactatcaa ccatctcgtg cggtccgagg
     8461 atccacccga tgcggagtcc cggcaggccg tacaccttcg acaggcctcc ggtgacgagc
     8521 gcccgcgcgt accgcccccg gaatgacggg ctggtatgac catcgcgctc gccgccgcgg
     8581 tacacttcat ccgccacgac ccacgcattg acctttgctg ccaactcgca gatcgtgttc
     8641 atcgcctcga cggtgagcac ggcgcccgtg gggttgttgg ggttgcagat gaaaatcgcc
     8701 ttcgtacgcg gcgtgacgag gcgggcgagg tcgtcgagat cgggacccca ctggttcgct
     8761 tccgtcagcc accaggtctt tgcctcggcg cccaatcctc ggatgagcag cggaatctgc
     8821 atgtagttcg gcagcatcac cacagcttcg tcgcccggct gcagcagcgc ccaggtaatc
     8881 aggaagttgg cctccgcgct cccggaagtg acgaggacgt gctcgggtcc tgcggcgtga
     8941 tagagaccgg cgatctccgt gcggagctcg aggctgccgt ttgtcatgcc gtagccgatg
     9001 ggttcgtgcg cgaggacttc ctgcacccac gaatggtcga cgaggccgga gagagtcagc
     9061 ggtaacacgc cgctgtcggc gaggttgaac tcgacggtgt gctcccacgt cgattgaaac
     9121 cgctccatct cgaagcgctc gatcttcacg ggagttgccg gctccttgcg gtatccgcgt
     9181 taccgcgttg tcgcgttacc gccttcttcc gttcgcccgc ccgtttcgcc tgcgacctcc
     9241 gttgccggtt agcacgagtt ccgcctgctg cttcagcacg cgtctcagga actggccggt
     9301 gtatgagtcc ggcgcccggg ctacatcttc gggggtccct tcagcgatta cctggccgcc
     9361 aagctcgccg ccttccggcc cgaggtcgat gatccagtcc gcggtcttaa tgacatccag
     9421 gttatgctcg atcacgatca cggtgtttcc ggcatcaacg aggcggtgca gaacgctcag
     9481 cagccgctcc acgtcggcaa agtgcaggcc gacggtcggc tcgtccagga tatatacggt
     9541 ctggccggta tcgcggcgcg acaattcggc cgacaacttc acgcgctggg cctcaccgcc
     9601 tgacagcgtc gttgcgggct ggccgagctt gacgtaccca agccccacgt cctcgagggt
     9661 ctgtagcttt cgccggatcc gcggcacatt ctcgaagaac tgcaccgctt ctgcaaccgt
     9721 catctccagg acgtccgaaa tgctctttcc tttatagtgg acctccaggg tttcccggtt
     9781 gtagcgtttg cctttgcaca cctcgcacgg gacatacacg tccggcagga agtgcatctc
     9841 gatacgcacg atgccgtccc cttcgcacgc ctcgcagcgg ccgcctttga cgttgaagga
     9901 gaaccgcccg ggggtgtatc cccgcatccg ggcgtccggc gtctgcgcaa acaggtcccg
     9961 gaccaggtca aacgtcttcg tataggtcgc cgggttgctg cgcggcgtgc ggccgatcgg
    10021 cg
//
LOCUS       DBNGSB010000147         2499 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3628790, whole genome shotgun sequence.
ACCESSION   DBNGSB010000147 DBNGSB010000000
VERSION     DBNGSB010000147.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3628790"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..225)
                     /locus_tag="AC159B_04235"
     CDS             complement(34..225)
                     /locus_tag="AC159B_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376987.1"
                     /translation="MSRPVPTKDELMTLMKAKVTEARASLRAAAESLTLSAEQREEDE
                     KLISALDEIERKIQSLRES"
     gene            264..1262
                     /locus_tag="AC159B_04240"
     CDS             264..1262
                     /locus_tag="AC159B_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR3376988.1"
                     /translation="MQRTEDLTREELNSALEWYAKMVLARRFEDAAERAFRHGKIGGY
                     LHVYSGQEAVAAGFLSTLRKDDIFFTAYRDHAHALFSGSPPGAVLAELYGKATGLAKG
                     KGGSMHLFDAQRGFYGGYGIVGGHIPLATGAAYTLKYLDTERLCLCFLGDGAMNSGSF
                     HEPVNMAGLWGRDGLCPIVYIVENNQYAMGTSVSRSSAVSNLSSRFFAYEIENERADG
                     MDYFTVRRVASRVINQVRESGRPYAVEMLTYRFAGHGAADLFQPYRNRGEVSDARKRD
                     PITLLEDRLRAAGLLTDADVDRIQGEVEQAVEQAVKFADESPEPAAEELFKDVYGS"
     gene            1269..2249
                     /locus_tag="AC159B_04245"
     CDS             1269..2249
                     /locus_tag="AC159B_04245"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR3376989.1"
                     /translation="MPELTYRDALRSTLVEEMDRDRAVVLIGEDIGVYQGTFRVTADL
                     LERFGPKRVIDTPISELGFVGAAIGMAMLGLHPVVEVMTWNFALLAFDQIVNNAAKLR
                     YFSGGQVDIPIVIRGPNGAGVQLSAQHSQSLEATFAHFPGLFVVTPATPAEAKGLLRT
                     AIRGNNPVIFLENAALYGMKGEVPNGDFAIPFGRAEVARAGRDVTIVTYSRMLHLALA
                     SAAQLHEDGIDAEVINLRTLRPLDTETIVASVSKTHRAVVVQEQWKPFGAAGEIAAII
                     YENAFDELDAPVERVAGADVPMPYARNLELLATPHEGDIIKAVRKTLYKS"
     gene            2376..>2499
                     /locus_tag="AC159B_04250"
     CDS             2376..>2499
                     /locus_tag="AC159B_04250"
                     /inference="COORDINATES: protein motif:HMM:NF012584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl domain-containing protein"
                     /protein_id="HIR3376990.1"
                     /translation="MPKMGDAMTEGKVLKWHKRPGDQVRQGEPLAEIETDKVNVD"
BASE COUNT          480 a          765 c          781 g          473 t
ORIGIN      
        1 gaagacggcg gatcgattcc agggaggggg tgatcatgat tcccttaaac tctgaatctt
       61 gcgttcgatc tcgtcaagcg cactgatcag cttctcgtct tcctctcgct gctcggcgga
      121 cagggtgagc gactccgcgg cagcgcgcag gctcgctctg gcctccgtca ccttcgcctt
      181 catcaacgtc atgagctcgt cttttgttgg aaccggtctg ctcacgtgta aaggtatggt
      241 agtataacca ccgataacct gccatgcagc gcaccgagga cctcacccgc gaagagctga
      301 acagcgccct cgaatggtac gccaaaatgg tcctcgccag gcgtttcgag gacgccgcgg
      361 agcgcgcgtt ccgtcatggg aaaatcggag gctacctgca cgtgtactcc ggccaggagg
      421 cagtggcggc cggcttcctc tccacgcttc gcaaagacga catcttcttt acggcgtatc
      481 gcgatcacgc ccatgccttg ttcagcggct caccgcccgg tgcggtgctg gcggagctgt
      541 acggcaaggc caccggcctg gccaaaggca agggcgggtc catgcatctc ttcgatgcgc
      601 agcgtgggtt ctacggaggg tacgggatcg tcggcggcca cattcctctc gccaccggcg
      661 ccgcatacac cctcaaatat ctcgacaccg agcgtctctg cctgtgcttc ctgggcgacg
      721 gcgcgatgaa cagcggctcc ttccacgagc ccgtgaacat ggcgggcctg tggggacgcg
      781 acggcctgtg cccgattgtt tacatcgtcg agaacaacca gtacgcgatg gggacgtcgg
      841 tctctcgatc gtcggccgta tctaaccttt cctcgcgctt ctttgcgtac gagatcgaga
      901 acgaacgggc ggatgggatg gattacttca cggtcaggcg cgtggcctcg cgggtgatca
      961 atcaggtgcg ggagagtgga cggccctacg ccgtcgagat gctgacctac cgcttcgccg
     1021 gccacggcgc ggctgatctc tttcagccct atcgcaaccg cggtgaagtt tctgacgcgc
     1081 gcaagcgcga cccgatcacc ctgctcgagg accgcctccg cgcggcggga ctgctcaccg
     1141 atgcggacgt cgatcggatc cagggggaag tcgagcaggc ggtcgagcag gcggtcaagt
     1201 tcgcggacga gagtcccgag cccgcggccg aggaactctt taaagacgtg tacggctcgt
     1261 aaccccccat gccggaattg acataccgcg acgcgcttcg gtccaccctg gtcgaggaga
     1321 tggaccgcga ccgcgccgtg gtcctcatcg gcgaagacat cggcgtgtat caggggacat
     1381 tccgcgtcac cgcggatctt ctggaacggt ttggtcccaa acgtgtgatc gatacgccca
     1441 tctcggagct cgggttcgtc ggcgcggcga tcggcatggc catgctggga ctgcatccgg
     1501 tcgtcgaggt tatgacgtgg aactttgcac tgctcgcgtt cgatcagatt gtcaataatg
     1561 ccgcgaagct ccggtatttc tcaggcgggc aggtcgacat acccattgtg atccgcgggc
     1621 ccaacggtgc gggtgttcag ctatccgccc agcactcgca gagcctcgag gccacgttcg
     1681 cgcactttcc ggggttgttc gtcgtcacgc ccgcgacacc ggcggaagcg aaagggctgc
     1741 tccgcacggc gatccgcgga aacaatccgg tgattttctt ggagaacgcg gccctctatg
     1801 ggatgaaggg cgaggttcca aacggcgatt ttgcgatccc atttggccgt gccgaggtcg
     1861 cccgagccgg tagggacgtc actatcgtga cctatagccg gatgctgcac ctcgcgctgg
     1921 catcggccgc gcagctgcac gaggacggca tcgacgccga agtcatcaac ctgcggacgc
     1981 tgagacccct ggacacggag accatcgtgg cgtcggtctc gaagacacac cgcgccgtgg
     2041 tcgtgcaaga acagtggaag ccattcggcg cggcgggaga aatcgccgcg atcatctacg
     2101 aaaatgcctt cgacgaactc gacgcacccg tggaacgcgt cgccggagcc gatgtgccca
     2161 tgccgtatgc gcgcaacctt gaactacttg cgacgccgca tgagggagac attattaagg
     2221 cggtaaggaa gacgctctac aaatcgtaac aacgcgaacg aggcgaacat ggcgaacaag
     2281 gcgagcaagg ttactacaca cggactcgtt gcctcgttcg ctttgttcgc tacgttcgct
     2341 ttgttcccgt agttggaggg tttatggacg tgatcatgcc caaaatgggc gacgccatga
     2401 ccgagggcaa ggttctcaaa tggcacaagc gccccggtga tcaggtgcgg cagggcgaac
     2461 cgcttgcgga gatcgagacc gacaaggtca acgtcgaca
//
LOCUS       DBNGSB010000148         4385 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_103275, whole genome shotgun sequence.
ACCESSION   DBNGSB010000148 DBNGSB010000000
VERSION     DBNGSB010000148.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4385)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_103275"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..1024
                     /locus_tag="AC159B_04255"
     CDS             44..1024
                     /locus_tag="AC159B_04255"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3376991.1"
                     /translation="MRLKLAALVAATVLLTFAPGLAAPAYPEKALELIAPAGAGGGWD
                     LTARMTARALGEEKLVTVPVTVTNMPGGSGAVGIAHMVTRRKGDAHVLAVMGSSLTGT
                     LARKAVPYTFKDVIPIAALTGDFMVVAVRRDSPFTRLRVLMDVYRRDPGLITVGGGSA
                     PGSLDHIAFALLGKNVGVDTTKVRYVPFDGGAQAMAALLGGSITVLSTSLSEVLPNLE
                     AGQIRVLAVTAAERLGGNLRSIPTALEQGIDFVFVNWRGFYMPPDTPASVVRYWETTL
                     ERMVRSRAWAKILDEMKWAPFVLTGERFRRFLEEDLSSTEAALRELGLLR"
     gene            1037..1483
                     /locus_tag="AC159B_04260"
     CDS             1037..1483
                     /locus_tag="AC159B_04260"
                     /inference="COORDINATES: protein motif:HMM:NF018979.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HIR3376992.1"
                     /translation="MHSDRPAAIVLATVAALYIWEAREFRGVTVSDVVGPSAYPWLLG
                     GTLALLAIVLFARTPPSTAGGGFWSRHGRPAAFTGSLFAYCMALEPVGFLASTFVYLA
                     FSHRWLGEQRWVRAILIAAGATVALWVFFDRVLDVGLPAGAIGWPR"
     gene            1480..2973
                     /locus_tag="AC159B_04265"
     CDS             1480..2973
                     /locus_tag="AC159B_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013157235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR3376993.1"
                     /translation="MSDMFSSLIFGFGVALQPVNLLLAVIGAFVGTLIGVLPGIGPVS
                     GIALLIPLIIGLHPASAMIMLTAVYYGTMYGGSTTSILLNVPGEASSVATTLDGYQMS
                     LQGRGGPALAIAAIGSFIAGTLSVVAMMIFSLPLANWALAFGPPENFALMVLGLSSVS
                     TLAGQDLVKGLISTVGGLMLATVGTDLVTGINRYTLGIPQFLDGIDLVTVAVGLFAVS
                     EVLLAVNMITVQPRLIPLQRIWLTMKDFLESFWAIIRGTVVGFYIGILPAAGATIASF
                     LSYGLERRLARDPSTFGKGDIRGVAAPESANNAAAVGNMIPMLSLGIPGSSTTAVMMG
                     ALLILNVIPGPLLFTQHPDVVWGLIASMYVSNAVLLLLNLPLVGLFVQILKTPDRYLY
                     PLIIAICVIGVYTVSRSRVDLMLMILLGALGYYMRLNEYPLGPAILGLVLGDRMEQAM
                     RQSLIMSSGSPAIFVTRSLSAIVLALAVLSLAAPVLFRLLSSRRQTS"
     gene            complement(2993..3682)
                     /locus_tag="AC159B_04270"
     CDS             complement(2993..3682)
                     /locus_tag="AC159B_04270"
                     /inference="COORDINATES: protein motif:HMM:NF016895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR3376994.1"
                     /translation="MLVGAGAYWILRLERQLAEAPSPTEVTAPPRVSQGPIPATAERR
                     DIPKVRDGRVRAATLQSSDEPSAFATVYILEFGRFSNLSAARARAQQVRSKGYAAQVA
                     GGAALYRVIGREFRSQASAERWKGILEEIGLESRVSQQVVAQRTITVTKFAVDFGEFT
                     QRTAAETLARIVRSRGYLVKVASTGPGFQVLGRGHMDKTRAESWANLFREIGFEASVT
                     SIRELTQYPVN"
     gene            3956..>4385
                     /locus_tag="AC159B_04275"
     CDS             3956..>4385
                     /locus_tag="AC159B_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3376995.1"
                     /translation="MRNFLAFGVFALFVAAGMPAVRAGDTACIGVLAIGSYDNLKVTG
                     GVCVIGAGTHVLGNIDVEPGAGLFVNSGAIIRGSITKKGPGSLEIFGAAIGGNVQSQD
                     GSSFRLHSNSVRVNLELIGNNGFQQEIFANFVGGNLKYEKN"
BASE COUNT          795 a         1342 c         1335 g          913 t
ORIGIN      
        1 ggagacagcg cgaatgcgtt ttgttgaacc caggaggtgc atcatgagat taaagcttgc
       61 ggcgctggta gccgctacag tactgctgac gtttgccccc ggtctggccg cgccggccta
      121 ccccgaaaag gcgctggagt tgatcgcgcc cgcgggcgca ggcggcgggt gggacctcac
      181 ggcccgcatg actgcccggg cccttggaga agagaaactc gtcaccgtgc cggtcacagt
      241 gaccaacatg cccggcggta gcggcgcagt cggcatcgcg cacatggtca cgcggcgaaa
      301 aggtgacgct cacgtgctgg ccgttatggg gtcctcgctg acgggaacgc tcgcgagaaa
      361 agccgtcccg tacaccttca aggacgtcat ccccatcgcg gcgctgacgg gcgacttcat
      421 ggtcgtcgcc gtgcgccgag actcaccgtt cacgaggctg cgtgtgctca tggatgtgta
      481 ccggagggat ccgggactga tcaccgtcgg cggcggatct gcgcccggct cgctcgatca
      541 catcgcattt gccttgctcg gcaagaacgt cggcgtcgac acaacaaagg tgcgatacgt
      601 tccatttgat ggtggggcac aggcgatggc ggcgctcctc ggtggctcca tcacggtgct
      661 gtctacgagc ctctccgaag tattgccgaa cctcgaagcc ggacaaatcc gagtcctggc
      721 cgtgaccgcc gccgagcgcc tgggtggaaa tctccgatcg atccccacgg cccttgagca
      781 aggcatcgac ttcgtcttcg tcaactggcg aggattctac atgccgcccg ataccccggc
      841 gtcggtggtc cgctattggg agacgacact cgagagaatg gtcaggagtc gggcatgggc
      901 aaagatcctc gacgagatga agtgggcacc gtttgttctg accggggaac ggttcaggag
      961 gttccttgag gaggatcttt catcaacgga agcagccctg cgcgaactgg gactcctgag
     1021 gtagcgagct caccagttgc atagcgaccg tccggcggcg attgtccttg cgacagtcgc
     1081 cgccctctac atttgggagg cgcgagagtt tcgcggcgtt accgtctccg acgtcgtggg
     1141 tccgtccgct tacccgtggc tcctgggcgg cacgctcgcg ctgctggcga ttgtgctgtt
     1201 tgcgcggaca cctccatcca ccgcaggcgg aggtttctgg agccggcacg gccggcccgc
     1261 cgcgttcacc ggatcactct tcgcgtactg tatggcgctg gaacccgtgg gcttccttgc
     1321 cagcacattt gtatatctcg cgttttctca ccgctggctc ggggagcagc gctgggtccg
     1381 tgccatcttg atcgcagcgg gcgcgacggt cgccctgtgg gtcttttttg atcgcgtcct
     1441 cgacgtcggc cttccggcgg gcgcgatcgg gtggccgcga tgagcgatat gttcagcagc
     1501 ctaatctttg ggtttggcgt cgcgttgcag cctgtcaatc tgctgctggc agtgatcggc
     1561 gcgtttgtgg gcacgctgat cggagttctc cccggcatcg gaccggtcag cggcatcgcc
     1621 ctccttatcc ctctcatcat cgggctgcat cctgcctcgg ccatgatcat gctcacggcc
     1681 gtctattacg gcacaatgta cggcggctcg acgacgtcga ttctcctcaa tgttcccggg
     1741 gaagcatcat cggtcgcgac gaccttagat ggctaccaga tgagcctgca gggacgcggc
     1801 ggtccggcgc tggccatcgc agcgatcgga tcgttcatcg caggaacgct gagcgtggtg
     1861 gcaatgatga tcttctctct gcccctcgca aattgggcgc tcgcattcgg cccgcctgag
     1921 aacttcgcgc tgatggtcct ggggctgtcg agcgtaagca ccctggcggg ccaggacctg
     1981 gtcaaagggc tcatctctac ggtcggcggg ctgatgctgg ccaccgtggg caccgacctc
     2041 gtgacgggga tcaatcgcta tacgcttggg ataccgcaat tcctcgatgg catcgatctc
     2101 gtcaccgtcg ccgtgggctt gttcgcagtc tctgaagtac tgctcgcggt gaacatgatc
     2161 accgtgcagc cgcgattgat ccctctccag cggatctggc tcaccatgaa agacttcctc
     2221 gagagtttct gggcgatcat ccgcggcacc gttgtgggtt tctacatcgg catcctgccg
     2281 gcggccggcg cgacgatcgc gtcgtttctc tcgtatggat tggaacgccg cctggccagg
     2341 gatccctcga cgttcggcaa gggtgatatc cgcggggtgg ccgccccgga atctgccaat
     2401 aatgccgcgg ccgtcggcaa catgatcccg atgctgtcgt tgggcatccc cggttcgtcc
     2461 acgaccgcgg tgatgatggg cgcgctgctg atcctgaacg tgatcccggg gccgctcctc
     2521 tttacgcaac atcctgatgt cgtgtggggt ctgatcgcca gcatgtacgt cagcaatgcg
     2581 gtgctcctgc ttctcaacct tccgctggtg ggactgttcg tgcagatctt aaaaacaccc
     2641 gatcgatatc tctaccccct gattattgcc atttgcgtga ttggcgtgta cacagtgagt
     2701 cggagccggg tggacttgat gctgatgatc ctcctcggag cgctcggcta ctatatgcgc
     2761 ctcaacgagt acccgctcgg accggcgata ctcggactcg ttctgggtga ccgcatggag
     2821 caggccatga ggcaatcgct cattatgagc agcgggagtc ccgccatctt cgtgacgcgg
     2881 tcgctcagcg ccatagtgct cgccctcgcc gtcctgtcgc tcgccgcccc tgtgctcttt
     2941 cgactgctga gctcgcgcag gcaaacctct tgaagaccaa aattaatgag ggttagttca
     3001 cggggtactg cgtcaattcc ctgatcgagg tcacgctggc ttcaaaaccg atttctctga
     3061 agaggttcgc ccaggattcg gcgcgtgttt tgtccatatg gccgcgccca agcacctgga
     3121 acccgggacc cgtactcgct accttcacta agtatccccg gctccgaaca atccgtgcga
     3181 gggtctccgc cgcagtgcgt tgtgtgaact ccccaaaatc gacggcaaac ttcgtcacgg
     3241 taatggtccg ctgggccaca acctgctggc tcacgcgcga ttccaatccg atctcctcaa
     3301 ggattccctt ccagcgctcg gcgctggcct ggcttcgaaa ctcacggcca ataacacggt
     3361 acaaagccgc gcctcccgcc acctgcgcgg cgtagccctt gctgcgcacc tgttgtgcgc
     3421 gcgcccgggc cgccgacagg ttactgaagc gaccaaactc aaggatatag accgtggcaa
     3481 aggcagacgg ctcatccgat gattggaggg tcgcagcacg tacgcggccg tcccgaactt
     3541 tcgggatgtc gcgccgctcc gcggtcgcag gaatagggcc ttgagacacg cgggggggag
     3601 ccgtgacttc tgtaggcgat ggagcctccg cgagctggcg ctcaagacgc agaatccaat
     3661 aggcgcctgc gccaacaagc atgacggtta agacactgag ggaagcaatt acgagcgcga
     3721 aagcccgtcg ttcttggtaa cgatgcttag gggtgaactt cccgggctcc caggtggcca
     3781 taaccctttc aatcctccag atacccgcta ttagccgatt ctagccttca tcataggagc
     3841 ctgctacgcg gctgtctctc cttcaatcga agggtctttg gcagaaccat tcagatgaac
     3901 ctttccgtat ggctatcgag ggtcaaactg gtctggaccc aaacgaggtg cagcgatgag
     3961 aaacttcctt gctttcgggg tctttgcact gttcgtcgcg gcggggatgc cagccgttcg
     4021 cgcgggcgac acagcgtgca taggcgtact cgcgatcggg tcctacgaca acctcaaggt
     4081 gaccggaggg gtgtgtgtga tcggcgcggg cactcacgtg ctggggaata tcgacgtaga
     4141 accaggagca ggactatttg tgaactctgg cgcgatcata agaggcagca tcacgaaaaa
     4201 gggacccggc tccctggaga ttttcggagc ggccatcggc ggtaatgttc agtcacaaga
     4261 tggaagctcg ttccgcctcc acagcaacag cgtacgagta aacctcgaac tcatcggcaa
     4321 caacggcttc caacaggaaa tctttgcaaa cttcgtaggt ggcaacctca agtatgaaaa
     4381 gaacc
//
LOCUS       DBNGSB010000149         4097 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3170637, whole genome shotgun sequence.
ACCESSION   DBNGSB010000149 DBNGSB010000000
VERSION     DBNGSB010000149.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3170637"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="AC159B_04280"
     CDS             <1..266
                     /locus_tag="AC159B_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013012950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3376996.1"
                     /translation="IIGMTKSIAREVASRGITVNAVAPGFIDAGITQQLPREMAQSFI
                     EQVPLGRAGKPDEVAAAVVFLASDGAAYITGQVLNVDGGLVMH"
     gene            300..551
                     /gene="acpP"
                     /locus_tag="AC159B_04285"
     CDS             300..551
                     /gene="acpP"
                     /locus_tag="AC159B_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020560661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR3376997.1"
                     /translation="MAPVFDRVKGIVVDQLGVAAEEVKSEASFVEDLGADSLDVVELV
                     MALEEEFGIEIPDEQAEKIATVGEAVKYIEAHMTEAPAK"
     gene            577..1824
                     /gene="fabF"
                     /locus_tag="AC159B_04290"
     CDS             577..1824
                     /gene="fabF"
                     /locus_tag="AC159B_04290"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HIR3376998.1"
                     /translation="MEPKRVAITGLGVISPVGIGKDRFWNALMEGRSGVDRITRFDAS
                     GFDSKIAAEVRDFDPADYMDKKEIRRNDRFVQFAYAASRMALDDSGFSITPQNATQVG
                     VLIGSGIGGATTWEEQHRTLLERGPDRVSPFFIPMIIINMASGIISILLGAKGPNSAV
                     VTACATGGNAIGDAARNIQRGEAIAMLAGGSEAAITPLSMAGFCSMKAMSTRNDEPQK
                     ASRPFDAERDGFIMGEGSGVVLLEEMEHAKQREANIYAELVGYGMSGDAYHITQPDPE
                     ADGAARSMRNALRDAAMEPKQIDYINAHGTSTLYNDRTETLAVKRVFGEHAKKVAVSS
                     TKSMTGHLLGAAGGVELIACVQAIEHGVIPPTINYEFPDPECDLDYVPNAARKMRVDA
                     AMSNAFGFGGHNAILIVRRYDGH"
     gene            1814..2692
                     /gene="rnc"
                     /locus_tag="AC159B_04295"
     CDS             1814..2692
                     /gene="rnc"
                     /locus_tag="AC159B_04295"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HIR3376999.1"
                     /translation="MATEQNVPLPQDAPPQVDTERDNLLAELERKLDLRFRDRHLLNL
                     ALLHGSFGHEGHGGRRESYERLELLGDAVLNLVVCDHLYHRYPEAPEGELAKLRARIV
                     SEPWLARIAQALDLGRYIRLGKGEEKAGGRARPALLADVLEAVLGAIYVDAGFGVSHA
                     VSTKWFQETFEDLELPSDDYKSSLQELLQERERRLPRYRIRDEVGPDHAKVFVAIVEA
                     GGRTLGEGRGQSKKQAEQAAAQAALEYLRRRSARPPQSSRPPTPREAGPRRNRPPFRP
                     RHGPGSPPGQPEKRPR"
     gene            2689..3213
                     /locus_tag="AC159B_04300"
     CDS             2689..3213
                     /locus_tag="AC159B_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014788102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIR3377000.1"
                     /translation="MRRLWAPWRLQYVGAPKAEGCIFCAAASGNDDRKSLIVHRGPLA
                     FVILNLFPYNSGHAMVVPYRHLPRVGDLSDSEKLELMILLDLTMRAQEMAFHPEGTNV
                     GINLGRAAGAGIEDHLHIHVVPRWVGDANFMPVLGDTKVIPEHLEATYQRLHGAFAAL
                     TAAEPARRTELGGT"
     gene            3214..>4097
                     /locus_tag="AC159B_04305"
     CDS             3214..>4097
                     /locus_tag="AC159B_04305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3377001.1"
                     /translation="MRTVSQVMAKPVVTARPNETVSAVVERFRVHDVSGMPVIDGAQV
                     VGMVTLRDLLGQPSYRPIREVMGRHIITVPPAEAVTSAFALMDEYRLEQIPVVDQGVL
                     VGVVTRSGLMHELGKLTDPLTELPWASALRQQAANLLRTGREVSIVFVDLDGFGNVNK
                     QYGHVIGDRVIRAIADTLLILTDPQSDLLCRYGGDEFAIVTTRSASDCEDLAARLRAA
                     IGSVAVPGLPSGIVSASVGIAGGTRTTAREESHTEATVDDLITMASRASTVAKSTDRR
                     VVHGHTLDAERVGQGDGRR"
BASE COUNT          787 a         1261 c         1357 g          692 t
ORIGIN      
        1 gcatcatcgg aatgacaaag tcgatcgccc gtgaagtcgc ctcccggggg atcacggtga
       61 acgctgttgc gccggggttc atcgacgcgg ggatcacaca gcagttgccg cgtgagatgg
      121 cgcaaagctt cattgagcag gtaccattag gaagggcagg gaaacctgac gaggtggccg
      181 cagctgtcgt attcctcgct tccgacggcg ccgcgtacat taccgggcaa gtcctgaacg
      241 ttgacggcgg cctcgtcatg cactagccgg ccctataatt agacgggggt gggaacccaa
      301 tggcaccggt atttgatcgt gtgaagggca tcgtggtgga ccaactaggg gtcgctgcgg
      361 aagaagttaa atctgaggcg tcattcgtcg aagacctggg cgcggattcc ctcgacgtgg
      421 tcgagctcgt catggcgctc gaagaggagt tcggtatcga aatccccgat gaacaggcgg
      481 agaagatcgc tacggtcggc gaggcagtca agtacatcga agcgcacatg accgaggctc
      541 ccgcgaagta acaccggacc ctcaggtccg gcacgtatgg aacccaagcg cgtcgctatc
      601 acaggcctcg gtgtcatcag ccccgtcggg atcgggaagg accgattctg gaatgcgctg
      661 atggaaggcc gcagcggcgt ggaccggatc actcggttcg acgcctcggg gtttgattcg
      721 aagatcgcgg cggaagtgcg cgacttcgat cccgccgatt acatggacaa gaaagaaatc
      781 cgccgcaacg atcgcttcgt gcagttcgcc tacgccgcca gccgcatggc cctcgatgac
      841 tcggggttct ccatcacgcc ccagaacgcg acgcaggtcg gagtgctgat cggctcgggt
      901 atcggtggag ccacgacctg ggaagagcag caccgcacgt tgctcgagcg cggcccggac
      961 cgcgtcagcc ccttcttcat cccaatgatc atcatcaaca tggcctccgg gatcatttca
     1021 atcctgctcg gcgccaaggg tcccaacagc gccgtcgtga cggcatgcgc gacgggcggg
     1081 aacgcgatcg gcgacgcggc ccgcaatatt cagcgcggtg aggcgatcgc catgctggcc
     1141 ggcggttctg aggccgcgat caccccgctc agtatggccg gattctgttc gatgaaagcg
     1201 atgtccacgc gcaacgatga accgcagaag gcgtcacgtc ccttcgacgc ggagcgcgat
     1261 ggcttcatca tgggcgaagg ctcgggggtc gtgctgctgg aggagatgga gcacgccaag
     1321 caacgggaag ccaacatcta cgccgagctg gtcggctatg ggatgtccgg cgatgcgtat
     1381 cacatcacac agccggatcc ggaggcagac ggcgccgccc gcagcatgcg caacgcgctc
     1441 cgggacgccg cgatggagcc gaagcagatc gactacatca acgcacacgg gaccagtact
     1501 ctgtacaatg atcggaccga gacgctggcg gtgaagcgcg tgttcgggga gcacgcaaaa
     1561 aaggtggcgg tgagctccac gaagtcgatg accggtcacc tccttggggc ggccgggggc
     1621 gtggagctga tcgcctgcgt gcaggccatt gagcacggcg tgatcccgcc gacgattaat
     1681 tacgagtttc ctgatcccga gtgcgacctg gactatgtgc cgaacgccgc gcgaaagatg
     1741 cgcgtcgatg cggcgatgtc caacgccttc gggttcggcg ggcacaacgc catcctcatc
     1801 gtgcgcaggt acgatggcca ctgagcagaa cgttccgctg ccgcaggacg cacccccaca
     1861 ggtcgacact gagcgagaca atctactcgc cgagctcgag cgcaaactcg atctccgttt
     1921 tcgagaccgg catttgctga atctcgccct gctgcatggg tcgttcggac atgaaggcca
     1981 cggcggcaga cgagagtcgt acgagcggct ggaactcctg ggggacgcgg tgctgaatct
     2041 cgtcgtctgc gatcacctct accaccggta ccctgaggcg cctgagggag aactagcgaa
     2101 gctgcgtgct cgcattgtga gtgagccttg gcttgcgcgg atcgcccagg cgctggatct
     2161 gggacgctac atccggctcg ggaaaggcga agaaaaggcc ggcggacggg cccgacctgc
     2221 gctgctcgcc gatgtgctcg aggcggtgct cggcgccatt tacgtcgacg ctggctttgg
     2281 tgtgtcgcat gccgtgtcca cgaagtggtt ccaggagact ttcgaagacc tcgagttgcc
     2341 gagtgacgac tacaagagct ctctgcaaga actgctgcag gagcgcgagc gccgtctgcc
     2401 gcgctaccgg atcagagacg aggtaggacc ggatcacgcc aaggttttcg tggccatcgt
     2461 tgaggccggg ggacgcacac tcggagaggg aaggggacag agtaagaaac aagcggagca
     2521 ggctgctgcc caggccgctc tcgagtatct gcggcgccgc tcagcgcggc cgccccagag
     2581 ttcgcgcccg ccgaccccgc gcgaggccgg gccccgccgt aatagaccgc ccttccggcc
     2641 ccgtcacggg ccggggagcc ccccaggaca accagagaaa cgccctcgat gaggcggctg
     2701 tgggcgccat ggcgcctcca gtacgtcggg gcgcccaagg cagagggctg catcttttgc
     2761 gcggccgcat ccgggaacga tgatcgcaaa tccctgatcg tccaccgtgg ccccctcgcg
     2821 tttgtcatcc tgaacctctt cccgtacaac tccgggcacg ccatggtggt gccgtacagg
     2881 catctgccgc gcgtgggcga cttgagcgat tcggagaaac tcgaactgat gatcctgctg
     2941 gacctaacga tgcgtgcgca ggaaatggct ttccatcccg aaggaactaa cgtcggcata
     3001 aacctcgggc gggcagccgg agccggcatc gaggaccatc tccatatcca cgtcgtgccc
     3061 cgctgggtgg gcgacgcaaa cttcatgccg gtgctgggtg acacgaaagt tatccctgag
     3121 caccttgagg ccacctacca gcgattgcac ggcgccttcg cagcgctcac cgcggcagag
     3181 cccgcccgcc ggacggaact cggcggcacg taaatgcgga ccgtgtcaca agtcatggcg
     3241 aagccggtcg ttacggctcg ccccaatgaa actgtgtctg ccgtcgtgga gcgatttcgc
     3301 gtccacgatg tcagcggaat gccggtcatc gacggcgctc aggtggtggg catggtcacg
     3361 ctgcgcgacc tgctggggca accctcctac cggcccatcc gtgaagtcat ggggcgccac
     3421 atcatcaccg tgcctcccgc ggaggcggtg acctccgcgt ttgcgttgat ggacgagtac
     3481 cggctcgagc agatccccgt cgtcgaccag ggcgtgttgg tgggtgttgt gacgcgctcc
     3541 ggcctcatgc acgagctcgg caaactcacc gatccgctga cggaactgcc atgggccagc
     3601 gcgctgcgcc agcaggccgc aaacttgttg cgcacgggcc gggaagtgag catcgtcttc
     3661 gtcgacctgg acgggttcgg caacgtcaat aagcagtacg gtcacgtcat cggcgaccgc
     3721 gtcatccgag cgatcgcgga tacgctgctg atattgaccg acccgcagtc ggatttgttg
     3781 tgccgctatg gtggcgacga attcgcgatc gtcacgacgc gctcggcctc cgactgcgag
     3841 gatctggcgg cgcgtctgcg cgcggcgatt ggatccgttg ccgtgcccgg gctgccgtct
     3901 ggaatcgtca gcgcatccgt gggtatcgcc ggcgggacgc gtacaacggc acgtgaggaa
     3961 agccacaccg aggcgaccgt ggatgatctg atcacaatgg cgagtcgcgc ttcgacggtg
     4021 gcgaagagca ctgaccggcg ggttgtccac gggcacacgc tcgatgcaga gcgggtgggc
     4081 caaggggatg gacgccg
//
LOCUS       DBNGSB010000150         5929 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_1178246, whole genome shotgun sequence.
ACCESSION   DBNGSB010000150 DBNGSB010000000
VERSION     DBNGSB010000150.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 5929)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5929)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5929
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_1178246"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..956
                     /gene="folD"
                     /locus_tag="AC159B_04310"
     CDS             90..956
                     /gene="folD"
                     /locus_tag="AC159B_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004339541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HIR3377002.1"
                     /translation="MTAKILSGKVIAAEVEAEAAKAAAALTKQYGMAPALAAILVGDD
                     PASAIYVRRKAEACGRSGLRSETFHLPANASEPEVLALIDDLNARPDIHGILPQQPMP
                     EQIDPRTVFGRVDPRKDVDGLGPENMAALMIGRPQLVPCTPAGVMRMLQYAGIDPSGT
                     EAVVIGRSAIVGRPMALLLLHANATVTWCHTRTRNLAAHTQRADILVVAAGRPGLITG
                     GMVKPGAAVIDVGVNRRDGKLTGDVDFESASKVAGWITPVPGGVGPMTIAMLLLNTVR
                     AAERLIPAVHRV"
     gene            959..2323
                     /locus_tag="AC159B_04315"
     CDS             959..2323
                     /locus_tag="AC159B_04315"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HIR3377003.1"
                     /translation="MTLSHADAMAFIDGLLSRDRARVSTAETIKLRRVETLVNRLGAP
                     HRQVPAVLIAGTKGKGSTAVMLASILQSAGLRVGLYTKPHLVDYRERFRVNGMLIDEA
                     VVTDLVNRIRPHVEAMGLQTDGPPSYFEASVALAFLYFAQADVDLAVIEVGLGGRLDP
                     TNIAEPVLSVLTPVSFDHMDVLGLTLDSIAREKAGIVRPEVAVASAPQPPEAFAAIID
                     VCTRLRAKLWPVDEIMSWRPLKTSLYNQSFELRGHWREYGPLSLPLVGMHQITNAATA
                     VAAAEILGEQGFSLSREAIMRGLASVQWPARVEVLQERPFVVADVAHNPASIGALRET
                     LEQLFPDRRIILVFGMVATHDHRASTSLLAPLADVVIATTPLHIKPLPGHVLAEEAAR
                     YVKHVEVVDDRLEALHRALELAEPDDVVVITGSFFLVGDLREALLQSAPSTQSAQSTQ
                     STRT"
     gene            2386..3987
                     /gene="lysS"
                     /locus_tag="AC159B_04320"
     CDS             2386..3987
                     /gene="lysS"
                     /locus_tag="AC159B_04320"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HIR3377004.1"
                     /translation="MWVDVIVEDILKTRPSARHVINDAKSPSGPIHVGSLRGVILHDC
                     IARGLTAAGQPVQFLFGFDDYDPMDARPAALPAEFDRYLGIPISEVPSPSGQNKSFGH
                     YYGLEFVEIWKRVGAHPEVYWTSDMYKAGRFNESIRVALDRAENFIRIDREVSGSQKA
                     ERHPVQPICENCGRVGTTVVTGWDGRQVGYECRPDKVTWAQGCGHHGNRSPFDGGSKL
                     QYKAEWAAKWRILGVSIEGAGKDHMTKGGSHDVASAFAERIFDYKTPYPIPYEWFLVG
                     GRKMSSSKGVGVSAGELVAILRPELARFLMVRPHYRQQINFDPTGETIPNLYDEYDRA
                     AAAYFGDLEGRTLAETELIRDHARTFHYSRTEGEIPRCFRMRFAKVASLMQMPTVDLF
                     DTAAKEKGGSLTPEDRAELERRMEDAKRWLASYAPDHYRFQVQTPMPEVTLSSAQREF
                     LGRLANLVAERAWSGEELHSRIHVLKSEVGLNPKESFSAIYHVFLGKDSGPQAGWFLA
                     SLDRNFVLSRLREASAVGARGPGSR"
     gene            3991..4947
                     /locus_tag="AC159B_04325"
     CDS             3991..4947
                     /locus_tag="AC159B_04325"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HIR3377005.1"
                     /translation="MPDREVHVLVFTSVEERHLDLIREVDPRIRVIAVDRQRGIELAP
                     PVEIMVVGWNVPEDAVRRATKLRWIQSVGAGVDTLIVPEIMSREIVLTTSSGIHHSVV
                     EHVIAVMLVFTRRLHLALRDQLRRKWDRSRASGEDLLGKTLGILGLGTIGVEIAQKAH
                     AFGMRVVGMRRTAAPVPGVDRVVGPDGLSMVLQESDAVVVALPLTPQTRGLIGEEAFR
                     TMKPSALFINIGRGPIVQEHALISALREGRLAGAALDVFEREPLPADSPLYEFENVII
                     TPHVSGTSPAYMDRAVPLFCENLHRYLRGEPLRNMVDKELGY"
     gene            4989..>5929
                     /locus_tag="AC159B_04330"
     CDS             4989..>5929
                     /locus_tag="AC159B_04330"
                     /inference="COORDINATES: protein motif:HMM:NF012239.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR3377006.1"
                     /translation="MKSFPPERIRNVAVVGHGSTGKTSLVEALLFLAKATERLGKVDD
                     GTSITDFDAEEQRRKHTINASLAPLEWKDHKVNLIDAPGYPDFVGEVVGALRAVEGAI
                     VVVDAVSGVQVQTEVAWSLADRAGVSRVVVVNRLDRENASFDRTMESLRRKFGTKVAP
                     IQIPIGSEGSFSGVVDLLEMKAYTFKNGDAVSGDLPGDLQAAAAAAREKLMEAAAETD
                     DALVEKYLETGELTQEELTRGLHEGVKAGALIPVMCACATKTIGIPQILEEIVTLLPG
                     PLERPEVTATDARTKQQVAVAQKPDAPLAALVFKTVA"
BASE COUNT         1123 a         1755 c         1922 g         1129 t
ORIGIN      
        1 gcgaagcgaa gcaatctcgt acttgcatga gattgcttcg gctgctttgc agcctcgcaa
       61 tgacacgcga gattgtgtag attgagaagg tgaccgcgaa gattctatcc ggcaaggtaa
      121 tagcggccga ggtcgaggca gaggctgcga aggccgccgc cgcgttgacc aagcagtatg
      181 ggatggcgcc ggcccttgcc gccatcctgg tcggcgatga tcctgcgtcg gcgatttacg
      241 tccgacgcaa agctgaggcc tgcggccgct cgggcctacg ctcggagaca tttcacctgc
      301 ctgcgaacgc atccgagccc gaggtgctcg cgctgatcga cgacctcaat gcgcgccccg
      361 atatccacgg cattctgccg cagcagccga tgcccgagca gatcgatcca cgaactgtct
      421 ttgggcgggt cgatccccgc aaagacgtcg acgggctggg cccggaaaac atggccgcgc
      481 tgatgatcgg ccgtccacag ctcgtaccat gcacacccgc gggtgtcatg cggatgctgc
      541 agtacgcggg aatcgatccg agcggaacag aagccgtcgt gatcggacgc agcgcaattg
      601 ttggcagacc gatggcgctc ctgctgctgc acgccaatgc cacagtgacc tggtgtcaca
      661 cccgcacgcg gaaccttgcc gcgcacacgc agcgagccga catcctggtc gtcgcagcgg
      721 ggcgtccggg attgatcacc ggcggcatgg taaaaccggg cgcggccgtg atcgacgtcg
      781 gggtcaaccg cagggacggc aagttgaccg gcgacgtcga cttcgagtcc gcgtcgaaag
      841 tcgccggctg gatcacgccg gtgccgggcg gtgtgggccc gatgacgatt gccatgctgt
      901 tactcaacac cgtgcgcgcg gcggaacggc tgatccccgc cgtgcacaga gtgtgaatat
      961 gacactctcc cacgctgacg ccatggcgtt catcgacggt ctgctctcgc gcgaccgcgc
     1021 acgggtttcg accgccgaga cgatcaagct gcgccgagtt gagacgctcg tgaaccgtct
     1081 gggggctcct caccggcagg tgccggcggt gctgatcgcc ggcaccaaag ggaaggggtc
     1141 caccgccgtc atgctggcct cgatcctgca gtcggcggga ctgcgcgtgg gcctctacac
     1201 taaaccgcac ctggtggatt acagggaacg ttttcgcgtc aatgggatgc tgatcgatga
     1261 agccgtggtg accgatttgg tgaatcggat tcgtccccat gtcgaagcaa tggggctcca
     1321 gacggacggg ccacccagct actttgaagc gtcggtcgcg cttgcgtttc tttatttcgc
     1381 gcaggccgac gtagatctcg ccgtcatcga ggtcggtctc ggtggccgcc tcgatcccac
     1441 caacatcgcg gagcctgtgc tgtcggtgtt gacgccggtg agtttcgatc acatggatgt
     1501 gctcgggttg acgctcgact cgattgcgcg cgagaaggcc ggcattgtgc gtcctgaagt
     1561 tgcggtggca agcgcgccac aacctcccga ggcgttcgca gcaatcattg atgtctgcac
     1621 gcgcctgagg gcgaaactgt ggcccgtcga tgagattatg tcatggcgcc cactaaagac
     1681 ttccctgtac aaccagtcgt ttgaattgcg tggccactgg cgtgagtacg gcccgctgtc
     1741 gttgccgctc gtaggaatgc accaaatcac caacgccgcg accgcggtcg cggctgcgga
     1801 aatccttggc gagcaagggt tctccctcag ccgggaggcg atcatgcgag gtctggcctc
     1861 ggtccaatgg cctgcgcgtg tggaagtgct gcaagaacga cctttcgtgg ttgcggatgt
     1921 ggcgcataac cccgcttcga tcggcgcgct tcgtgaaacg ctcgagcagc tcttccccga
     1981 ccggcgcatc atcctggtct tcgggatggt ggcgacgcat gaccaccgcg catcaacaag
     2041 tttgctcgca ccgctcgccg acgtcgtgat cgccaccact ccgctgcaca tcaagccgct
     2101 gccgggtcac gtcctggccg aggaggccgc ccgttacgtc aagcacgtgg aagtggtcga
     2161 cgaccgacta gaagcgctgc atcgtgcgct cgagctcgcg gagcctgatg atgtcgtcgt
     2221 tatcacaggg tcgttcttcc tcgttggaga tttgcgcgag gcgctcctgc aatctgcacc
     2281 atctacacaa tctgcacaat ctacacaatc tacgagaacc tgaaacgagg ggtatgatga
     2341 ggattttgtt gatcgtgcag attgtgtaga tcgtgaggat agcgtatgtg ggttgatgtc
     2401 atcgtcgagg acattctgaa gacgcgcccc tcggcccgcc acgtgatcaa cgacgcgaaa
     2461 agcccgtctg gcccgattca cgtcggatca ctgcgcgggg tgattctgca cgactgcatc
     2521 gcccgcgggc tgaccgcggc gggacagccg gtgcagtttc tctttggctt tgacgactac
     2581 gatcccatgg acgcccggcc ggccgcgctg cccgcggagt tcgatcgcta tctcgggatt
     2641 ccgatctcgg aagtcccgtc tccatccggc cagaacaaga gctttggtca ctattacggg
     2701 ctcgagttcg tcgagatctg gaagcgcgtg ggcgcgcatc cggaggtgta ttggaccagc
     2761 gacatgtaca aggccggtcg attcaatgag tcgatccgcg tcgctctcga ccgcgccgag
     2821 aacttcatcc ggatcgaccg tgaggtcagc gggagccaga aggccgagcg ccacccggtg
     2881 caaccgatct gcgaaaactg cggccgtgtc ggcaccaccg tggtgacggg gtgggacgga
     2941 cggcaggttg gttacgagtg ccgccccgac aaagtgacgt gggcgcaggg gtgtggacac
     3001 cacgggaacc ggtctccatt cgacggcgga agcaaactgc agtacaaggc agagtgggca
     3061 gccaagtggc gaattctggg cgtgtcgatc gaaggggccg ggaaggacca catgaccaag
     3121 ggtggcagcc acgatgtcgc ctccgctttc gccgagcgca tcttcgacta taagacgcca
     3181 tacccaatcc cgtatgagtg gttcctcgtt ggcgggcgga agatgagctc gagcaaaggc
     3241 gtcggcgtct ccgcggggga gctcgtcgcg attctgcggc cggaactggc acggtttctc
     3301 atggtgcggc cccactacag gcagcagatc aacttcgatc ccaccggcga aacgatacca
     3361 aatctctacg acgagtatga tcgcgccgct gcggcatact tcggggacct cgagggccgc
     3421 actctggcgg aaaccgaact gatccgtgat cacgcgcgaa cctttcatta ctcacgcacc
     3481 gaaggcgaaa taccacgatg tttccgaatg cggtttgcta aagtagcgtc cctgatgcag
     3541 atgcctaccg tcgatctctt cgacacggcg gccaaagaga aaggcggttc actgacccct
     3601 gaggatcgcg ccgagttgga gcggcgaatg gaggatgcga agcgctggct ggcgtcatac
     3661 gccccggacc attaccgctt tcaagtacag acgcccatgc ccgaggtgac gctctcgtcg
     3721 gcccagcgag agtttctcgg acgtctcgcc aatctcgtcg ctgaacgcgc ctggtcagga
     3781 gaagaacttc acagccggat tcatgttctg aaatctgagg ttggcttgaa cccgaaagag
     3841 tcattctcgg ctatctacca tgtcttcctc ggcaaggatt cgggaccgca ggccggctgg
     3901 ttcctggcgt cgttggatcg aaactttgtg ctgtctcgtc ttcgggaggc gtcggcggtc
     3961 ggagcccggg gcccggggtc ccggtagtcg atgcctgatc gggaagtcca tgttctcgtc
     4021 ttcaccagcg tcgaagagcg ccatcttgac ctgatccgcg aagtagaccc gcgcattcgc
     4081 gtgatcgcag ttgaccgcca gcgcggcatc gagcttgccc cgcccgtaga gattatggtg
     4141 gtgggatgga acgtgccgga ggacgccgtc cggcgtgcga ccaaattgcg gtggatccaa
     4201 tcggtaggag ccggcgtcga tacccttatc gttcccgaga tcatgtcgcg cgagattgtg
     4261 ctgaccacca gcagcgggat ccatcactcg gtcgtggagc acgtcatcgc ggtcatgctg
     4321 gtctttacac gtcggctcca tctggcgctc cgtgatcagc tgcggcgaaa atgggatcgc
     4381 agccgggcat ccggcgagga cctgcttggg aagacactgg gcatcctggg cctggggacg
     4441 attggtgtag agatcgctca aaaggcgcac gcgtttggca tgcgggtcgt cgggatgaga
     4501 cgaacggccg cgccggtacc aggcgtggac cgggtggtgg gccccgacgg tttaagtatg
     4561 gtccttcagg agtccgacgc ggttgtcgtg gccctgccgt tgacgccgca gacgcggggt
     4621 cttatcggcg aggaggcgtt tcgaacgatg aagccttcgg cgctcttcat taatatcggc
     4681 cgaggcccaa ttgttcagga acatgcgctg atctccgcgc tgcgcgaggg gcgtctcgcg
     4741 ggtgcagcgc ttgatgtgtt tgaacgggaa ccgctcccgg ccgacagtcc gctgtatgag
     4801 ttcgagaacg tgatcattac cccgcacgtg tcgggcacct cgccggctta catggaccgg
     4861 gcagtgccgc ttttctgcga gaatcttcac cggtatttgc gtggggagcc cctccgaaat
     4921 atggtcgata aggaactcgg gtattgagtg aagaaacctg atagtgatcc ctgagtggag
     4981 gtccaagtgt gaaatcgttt ccgcctgaga ggatccgaaa tgttgctgtt gtcggccacg
     5041 gcagcacggg gaagacgtcg ctcgtcgagg cgctgctgtt tctggcaaag gcgacggaac
     5101 ggttgggcaa ggtcgacgac ggcacatcaa taaccgactt tgatgctgag gagcagcgcc
     5161 gaaagcacac catcaacgcg tccctcgcac cactggaatg gaaagaccat aaggtcaatc
     5221 tcatcgacgc tccgggatat cctgacttcg tcggagaggt cgtgggcgcg cttcgcgccg
     5281 tcgagggcgc gatcgtggtg gtcgacgccg tttcgggagt ccaagtgcag acggaggtcg
     5341 cgtggagtct cgcggatcgc gccggcgtca gccgcgtcgt cgtggtcaac cggctggatc
     5401 gagagaatgc cagcttcgat cgaaccatgg agtcgctgcg caggaaattc ggcacgaaag
     5461 tggcgccgat ccagattccc atcggatcgg agggctcatt ttccggcgtc gtcgatctcc
     5521 tcgagatgaa agcctacacg ttcaagaacg gagatgcggt gtcgggggat cttcccggcg
     5581 acttgcaggc agcggcggct gccgcccgtg agaaactgat ggaggccgcg gctgagaccg
     5641 acgacgcgct cgttgagaag tatctcgaga cgggcgagtt gacccaggaa gagctgacgc
     5701 gcgggctgca cgaaggcgtc aaagccggtg cactcattcc ggtgatgtgc gcgtgcgcca
     5761 ccaagacgat cggaatccca caaattcttg aagagatcgt caccctgctt cctgggcccc
     5821 tcgagcgccc ggaggtcacg gcgaccgatg ctcggacgaa gcagcaggtg gccgtcgcgc
     5881 agaagccgga cgcaccgctg gccgcgctcg tgttcaagac cgtggccga
//
LOCUS       DBNGSB010000151         5331 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_1945364, whole genome shotgun sequence.
ACCESSION   DBNGSB010000151 DBNGSB010000000
VERSION     DBNGSB010000151.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 5331)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5331)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5331
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_1945364"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="AC159B_04335"
     CDS             complement(<1..255)
                     /locus_tag="AC159B_04335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR3377007.1"
                     /translation="MNQVILVGRLARDPELRVTGEGTPRAWFVLAVPKAFAGKDGDRD
                     ADFINIVTWRQLATTVGEHLKKGRMVGVTGRLQVSQLETGD"
     gene            complement(388..1131)
                     /locus_tag="AC159B_04340"
     CDS             complement(388..1131)
                     /locus_tag="AC159B_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012895512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HIR3377008.1"
                     /translation="MTWIFDPEIWIALVTLTVLEVVLGIDNIVFLSILAGKLPSEQQG
                     RARRLGLVLAMLMRIALLTTIAWIVRLTQPLFEVFEHAFSGRDLILIGGGLFLIAKST
                     REIHDKLEGEPGHVSARIPARFRAVITQIVLLDIVFSLDSVITAVGLVRDLWVMITAV
                     VVAVAVMMVAAGPISAFVERRPTVKMLALSFLLLIGLSLLVDGFGQHISKGYIYFAMA
                     FSIFVEMLNLRLRSRQGQPVELHQPFTAN"
     gene            complement(1128..2123)
                     /locus_tag="AC159B_04345"
     CDS             complement(1128..2123)
                     /locus_tag="AC159B_04345"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3377009.1"
                     /translation="MAEQGTVVVERFESQALKGNPLRDSNVREIPVYLPPSYERDRSR
                     RYPVIYWLHGFTGIGLSSINRSLWTPTLPELMDRVIADGAPEAVLVMPDGSTRYGGSQ
                     YINSPATGRYEDHMIKELVPYVDATYRTKASAGHRGTVGKSSGGYGALVLAMRNPDIF
                     GAVASHSGDMYFEAGYKPFFWKALNVINKHGGVRSFLNAFDAMLKKDREATEALTILV
                     AMAMAYSGNADGTYELPVDVKTGEMRDAIWSKWLEWDPVYMAERHAEALRKMKLVYIE
                     CGRKDEWNLHYGARILSGRLKALGINHEHQEFDDDHGNIQYRYVESLRRLSQVLA"
     gene            2281..3123
                     /locus_tag="AC159B_04350"
     CDS             2281..3123
                     /locus_tag="AC159B_04350"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3377010.1"
                     /translation="MQQQTMTDDDRFSFERFARHPFFRQVNAWLVAHTDISSNASVVD
                     LACGPGAVTELILGELDEEAPGACVYAVDPSSSALAIAQKRIRSRIVHFVEGSAERLA
                     SLVPQVDRVVFTNAVHLIADKAAVFEQIFKTLRPGGLVSFNTTFFQGAYPEGTSRFYK
                     LWVLRAMLWLKERGYSISRGVKATAMQWLTVEQYREVLRSVGFEDPVVTLHEKPLSLE
                     SLEDIGSFSLFIEGALPGVPLEMGAEALRQGVNQAFQELQLSVMPRNWLQVIARRPLT
                     PLAE"
     gene            complement(3137..3592)
                     /locus_tag="AC159B_04355"
     CDS             complement(3137..3592)
                     /locus_tag="AC159B_04355"
                     /EC_number="5.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HIR3377011.1"
                     /translation="MRTYTIQDRLEWTITPELYEQIRALWIKHSKAEDARDLQGLIDT
                     LASDCVYEIIPTGQRWERHDGARQFYLSFLGAFPDVKFAMSDIVIGPQGVFEVTEMSG
                     THKGTWAGMTPTGRTLKLQIVIHFPWDPKAEKFAGERIYFDRVALTEQL"
     gene            complement(3592..4566)
                     /locus_tag="AC159B_04360"
     CDS             complement(3592..4566)
                     /locus_tag="AC159B_04360"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HIR3377012.1"
                     /translation="MLKTAFTDLVGCPIPIQQAGMGTLAHPRLAAAVSNAGALGMVSV
                     AGAPADLLTEVLDRVRQETTAPFGCNFLMPFVGEDVRECVAIAASRARLIDFFWGEPD
                     RSLVDIVHEEGALACWQVGSRDEAVAAAKAGCDLIVAQGIEAGGHVRGRVSLLALLNE
                     VLEAVDVPIIAAGGVGNGRAMAAALAAGADAVRVGTRFVSAVEAEAHELYVSALIRAE
                     AADTVYTEAFSVGWPDAPHRVLRTSLQAAEAVQDDIIGERVGRYTGKKFPVHRLEIGV
                     PTITTTGAIEAMPHWAGESVGGVRRVQPAADIVREMSAEAEELLKRWC"
     gene            complement(4633..4863)
                     /locus_tag="AC159B_04365"
     CDS             complement(4633..4863)
                     /locus_tag="AC159B_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3377013.1"
                     /translation="MNLFRSEEHARAWSQFNPSSDEGLIDLRSLAAVFRTPSRRHLLD
                     QNYMSRWYPKRYVEWRAELERIGKATAYWGVT"
     gene            complement(4870..>5331)
                     /gene="merB"
                     /locus_tag="AC159B_04370"
     CDS             complement(4870..>5331)
                     /gene="merB"
                     /locus_tag="AC159B_04370"
                     /EC_number="4.99.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF015216.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018836 - alkylmercury lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="organomercurial lyase"
                     /protein_id="HIR3377014.1"
                     /translation="TIQLLDRTFYAVMKGFVEEGRAPHFTKLAIILGLEVEAGRQAFH
                     AVINAFAPSWLFPGTDLIASFAPFNNLPTQYGVTVRGLQKWFAQCGFEALAITWLFPG
                     ETVRIDAPCLDCGGPIAVEMRDGEFVRVEPPAVVGHLNYPLERSKEKLPFI"
BASE COUNT         1076 a         1701 c         1513 g         1041 t
ORIGIN      
        1 atcaccagtt tccagctggc tgacttgaag acggcccgtc acacccacca tccttccttt
       61 cttaaggtgc tctcctacgg tcgtcgccag ctggcgccag gtgacaatat tgatgaaatc
      121 ggcgtcccgg tctccatcct tgccggcgaa cgctttcggc acggcgagga caaaccaggc
      181 tctcggggtc ccctctccgg tcacacgcaa ctcaggatct cgggccagcc ggccgaccag
      241 aataacctgg ttcacgcctc ggcctgctcc ccgcgacgac gttgcgcccg tttcaccttc
      301 cccctgtgtc gccatctcag cactcctttc ctttccgctg gcatgacgat acccgggaac
      361 tggcgccggg gctatagccc aaatgagcta gtttgctgtg aagggttggt ggagttctac
      421 gggctggccc tgccgcgatc ggagacgcag gttcagcatc tcgacgaata ttgagaaggc
      481 catcgcgaag tagatgtagc ccttgctgat gtgctggccg aacccatcga cgagcagcga
      541 tagcccaatg aggagaagaa agctgagggc aagcatcttc acggtcggac gccgctcgac
      601 aaacgcgctg attggacccg cggccaccat catcacggcg acagcgacga ccaccgccgt
      661 gatcatcacc cagagatccc gcaccaagcc gacggcggtg atgaccgaat ccagcgagaa
      721 gacgatatcc agtagcacga tctgagtgat gacggcccga aacctagccg ggatccgcgc
      781 ggaaacatgg ccaggttctc cttcgagctt gtcatggatc tcgcgcgtgc tcttggcgat
      841 cagaaacagt ccacccccaa tgagaattaa gtctctgccg gagaatgcgt gctcaaagac
      901 ctcaaagagc ggctgggtca accgcacgat ccacgcgatg gtcgtgagga gcgcgatccg
      961 catgagcatc gcaaggacaa gaccaagacg gcgcgcccgg ccctgctgct ccgacgggag
     1021 tttgcccgcg aggatcgaca agaacacgat gttgtcgatc cccaagacaa cctccagcac
     1081 cgtcagcgta acgagcgcaa tccaaatctc gggatcaaaa atccaggtca tgcgagcacc
     1141 tgggaaagcc gtcgcaggga ttcgacgtag cggtactgga tgtttccgtg gtcgtcatcg
     1201 aattcttgat gctcgtggtt gatgccgagt gctttgagcc ggcccgacag gatgcgcgcc
     1261 ccgtagtgca ggttccactc atccttgcgg ccgcactcga tgtagacgag cttcattttc
     1321 cgcagcgcct ctgcatgacg ctcagccatg tagaccgggt cccattcgag ccacttgctc
     1381 cagatggcgt cccgcatctc gccggtcttc acgtcgaccg gtagctcata cgtcccatcg
     1441 gcgttcccag agtaggccat ggccatggcc acgagaatgg tcagtgcttc cgtcgcctcg
     1501 cgatccttct tcagcatcgc atcaaatgcg ttgaggaagc tgcgcacccc gccgtgcttg
     1561 ttgatgacat tcagcgcctt ccagaagaac ggcttgtagc ccgcctcgaa gtacatgtct
     1621 ccgctgtgcg acgctaccgc gccaaatatg tcgggatttc gcatggccag caccagtgcg
     1681 ccatatcctc cgctggactt tccgacagtg cctcggtggc cggcagacgc cttcgttcga
     1741 tacgtcgcgt cgacataggg gacaagttct ttgatcatat gatcctcata tcggccggtt
     1801 gcgggcgagt tgatgtactg actgccgccg taacgcgtcg acccgtcggg catcaccaac
     1861 acggcttcag gcgcaccgtc cgcgatcacg cgatccatga gctcaggcaa cgtcggagtc
     1921 cagagagagc ggttgatcga agacaacccg atcccagtga agccgtgtag ccaatagatt
     1981 acggggtatc gtcgcgatcg gtcccgttcg tacgacggtg gtagatacac ggggatttcg
     2041 cgcacgtttg aatcgcgcaa aggattccct ttgagggcct ggctttcaaa acgctcgacg
     2101 actacggtgc cctgctcagc catgctcttc tcctcccgaa cttcatcaga tcacagcggg
     2161 ccgggtggac ttttcgggaa tggtataggg aatcccgctg acacaatagg cagcaggaag
     2221 cggagtcgcg cgggcgaagg cctgatagcc agtcggccga tcggaatcgg agggagcgtc
     2281 gtgcagcagc agacgatgac ggacgacgat cgcttctcgt tcgagcgctt tgctcggcat
     2341 ccgttcttcc gccaggtcaa cgcgtggctg gtggcccaca cggacatctc ttccaatgca
     2401 tccgtagtcg acctcgcctg cggtccgggg gccgtgactg aactcatcct cggggaactc
     2461 gacgaagaag ctccaggagc ctgcgtgtac gccgtcgacc catcatcgtc ggcgctggcc
     2521 attgctcaga agcgtatccg gtctcgcatc gtgcattttg tcgaggggag cgcggagcgg
     2581 ctcgcaagcc tggtgccgca agttgaccgc gtcgtcttca cgaatgccgt gcatctcatc
     2641 gccgacaaag ccgccgtgtt cgaacaaatt ttcaagacgc tgcggccggg cggcctggtg
     2701 agtttcaaca ctacgttctt ccagggggcc tatcctgaag gcacctctcg cttctataaa
     2761 ttgtgggtgc tccgagcgat gctctggctc aaggagcggg gttactcgat ctctcgaggc
     2821 gtgaaagcga cggccatgca gtggcttacg gtggaacagt accgcgaggt tctacggtcc
     2881 gttgggttcg aagaccccgt cgtcaccctg cacgagaaac ccctgagcct tgaaagcctg
     2941 gaagacatcg gttcgttctc actgttcatc gagggcgccc ttcctggggt gccgctcgag
     3001 atgggcgctg aggccctgcg acaaggcgtg aaccaggcgt ttcaggagtt gcagttgtcg
     3061 gttatgccgc gcaactggct gcaggtcatc gcccgtcgcc ccctgacccc gctggccgag
     3121 tgagcaagaa aatctgctag agttgctcgg ttagggcgac gcggtcgaaa tagatccgtt
     3181 caccagcgaa cttctccgcc ttcggatccc acggaaagtg aatgacgatc tgcaacttga
     3241 gagttctgcc ggttggagtc attcccgccc acgttccctt gtgcgttccg gacatttccg
     3301 tcacctcaaa tacaccctgc ggaccgatca cgatgtcgga catggcgaac ttcacgtcgg
     3361 ggaaagcgcc gaggaacgag aggtaaaatt gccgtgcgcc gtcatgccgt tcccagcgct
     3421 gaccggtcgg gatgatttcg tacacgcagt ctgaggctaa cgtgtcaatc agcccctgca
     3481 gatctctggc atcttccgcc ttggagtgtt tgatccacag cgcccggatc tgttcataca
     3541 actccggcgt gatcgtccac tcgagacggt cctggatcgt gtaggtgcgc atcagcacca
     3601 ccgcttgagc agttcctcgg cttcagctga catctctcgg acgatgtccg ctgccggctg
     3661 cacgcgtctg acgccaccca cggactcccc ggcccagtga ggcatggcct cgatcgcacc
     3721 ggtcgtggtg atcgtaggaa cgccaatctc gagtcggtga actgggaatt tcttccccgt
     3781 gtacctcccc acgcgctccc caatgatatc gtcctgcaca gcctcagctg cctgaagaga
     3841 tgtacgcagg actcggtgtg gcgcgtccgg ccaacccacc gaaaaggcct ccgtataaac
     3901 cgtatcagct gcctcggcac ggatcagagc cgaaacgtaa agctcgtgcg cttcagcttc
     3961 gacggccgac acgaagcgcg tgcctacccg aacggcatcg gccccggcgg cgagcgctgc
     4021 cgccatcgcg cgtccgttcc ctaccccacc ggctgcaata atgggaacgt ccaccgcttc
     4081 gagcacctca ttgagaagtg cgaggagact gacccgcccg cggacgtggc cgcccgcttc
     4141 tattccctgc gcgacaatca ggtcgcatcc ggccttagcc gcagcgaccg cttcatctct
     4201 cgatcctacc tgccagcagg ccaacgcgcc ttcctcgtgg acgatgtcga caagtgaccg
     4261 atccggctcg ccccaaaaga agtcaatgag acgcgcgcga gatgcggcaa ttgccacgca
     4321 ctcccgaacg tcctctccca cgaacggcat caggaagtta cagccgaatg gagccgtagt
     4381 ctcttgccgg acccggtcca ggacctcggt cagcagatcc gcaggagctc ccgcaaccga
     4441 aaccatgccc agcgcacccg cgttcgacac ggcggcagca agcctcggat gcgccagtgt
     4501 acccatcccc gcctgctgga tcggaatggg gcagccgact aggtccgtga acgccgtctt
     4561 gagcattgtc tcctccttag gcacttcgct tccctcttta agccactccc ggcagcgacc
     4621 gacctgacta aattacgtta cgccccagta ggcggtcgcc ttgccgatgc gctcaagctc
     4681 cgcgcgccac tcaacatagc gtttcgggta ccacctcgac atgtaattct gatcgagaag
     4741 atgccgccgg ctcggtgtac gaaacaccgc agccaaactc cgtaggtcga ttaggccttc
     4801 gtcagacgac gggttgaact gcgaccacgc acgcgcatgc tcttccgacc ggaagaggtt
     4861 catgccgctt cagatgaacg ggagcttctc cttcgagcgc tccaaggggt agttcaggtg
     4921 gccgaccacg gctggaggct ccacacgtac gaactcacca tcacgcatct cgacggcgat
     4981 gggcccaccg caatccagac atggggcgtc gatgcgcacc gtctcacctg gaaagagcca
     5041 ggtgatcgcc agcgcctcaa atccacactg cgcgaaccac ttctgcaggc cgcgaactgt
     5101 gacaccgtac tgagtcggca ggttattgaa tggcgcgaaa gacgcgatta gatccgtgcc
     5161 cggaaacagc cagctgggag caaaggcatt tatgaccgca tgaaaggcct ggcgccccgc
     5221 ttccacttcc agccccaaga tgattgcgag ttttgtgaag tgcggtgccc gtccttcttc
     5281 gacaaaaccc ttcatgactg cgtagaaggt gcggtccagt aactgaatag t
//
LOCUS       DBNGSB010000152         9774 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2711152, whole genome shotgun sequence.
ACCESSION   DBNGSB010000152 DBNGSB010000000
VERSION     DBNGSB010000152.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 9774)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9774)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9774
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2711152"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..1107)
                     /locus_tag="AC159B_04375"
     CDS             complement(100..1107)
                     /locus_tag="AC159B_04375"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR3377015.1"
                     /translation="MQEIPESVFVFLIGAALASIAGALWVYRDSRARFGRRRNEPLLW
                     AIGTLLLLVLVLPFYLLFARPPGGVARCPACGSATLSHRATCLHCDNPIPFDSRPAMW
                     GLGEIVGIALVFGLTLPAIAASLGIGEASEFREFSIYAVVQSALLAALSLYVVTRRYH
                     QSADLLGLRADRLPALAGVGVLLAAAMVFISVGVEEVSIALVGAVIGRDRAKQMAEAE
                     HMRNPVTEILTSGVTASELIWVLLVLCVVVPVAEEIFFRGFVYRGLRARWGVLTAALA
                     SAAFFGVVHLEIVHFLPIFVLGVVVAYTFQRTGSLVPAIVIHALNNVVAVFGTIYRWD
                     I"
     gene            1302..3044
                     /gene="dnaX"
                     /locus_tag="AC159B_04380"
     CDS             1302..3044
                     /gene="dnaX"
                     /locus_tag="AC159B_04380"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HIR3377016.1"
                     /translation="MSHVSFYRKWRPKSFDEIIGQERVTRTLQNAIRANRVVHAYLFA
                     GHRGTGKTTTARILAKALNCIQGPTPTPDNTCPNCEAITAGYSVDVIEIDAASNRGIE
                     EIRELRDRIRLAPTEGRYKVYIIDEAHMLTTEAANALLKTLEEPPEHAVLILVTTEPH
                     RLPQTILSRCQRFDFRRVSQKELIARLRHIASTEGFEIEDKALTIIAGSADGSVRDAE
                     SVLDQLASYADGPIAARDVLMVLGILEEEIALKFADAIIERDVIASLGLVNQVVGEGK
                     DPRQITRTIMDHFRDLMVAKTADNPNELLDSTETRLQMLLSQATRAPVEDILRALNTL
                     VATEAEGRWSPQPRLLLEIALIRLCRPEMDPTLEGLRTRVQALEQRLVGESPSPKPAG
                     SKPALDPSAASGSGHVLNPSGANGSGHVLNPSGASGSGRTAAPTGVEPIRTQKAPVRD
                     EMKAEEGDGVTIVIGDVRRAWARVLEEVKRTKMFCHALLIEGTPVELNGSTLVVSLRT
                     GFNFHVENLHRPENRTVVEGALQRVFNHHLRLECTIHSEDTRPAARPSVEDPVVNAAM
                     QLFGAQVVDIKPVP"
     gene            3047..3361
                     /locus_tag="AC159B_04385"
     CDS             3047..3361
                     /locus_tag="AC159B_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014344564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HIR3377017.1"
                     /translation="MRDIGKMMKQVQKLQQDVARVQAELKDERVEATAGGGVVKAVAN
                     GHGEMQEIIIDPSVVDPGDVGMLQDLVLAAVNEAQRSAKQRAEEKMRAITGGVGLPPG
                     LI"
     gene            3499..4092
                     /gene="recR"
                     /locus_tag="AC159B_04390"
     CDS             3499..4092
                     /gene="recR"
                     /locus_tag="AC159B_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HIR3377018.1"
                     /translation="MYAEPLARLIEELSKMPTVGPKTAQRLAFYILRMTKEDAERLSQ
                     AILDAKARIHYCGICFHITDVDPCAICANPARSRSMICVVEDPRDVLAMERTREYRGL
                     YHVLHGAISPLDGVGPDDLKIKELLDRVKTGEVQEVIVATNPRVEGEATAIYLAKIIK
                     PLGMKVTRIAHGVPVGADLEYADEVTLARALEGRREI"
     gene            4177..4647
                     /locus_tag="AC159B_04395"
     CDS             4177..4647
                     /locus_tag="AC159B_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like YkuK family protein"
                     /protein_id="HIR3377019.1"
                     /translation="MEFVSPTVGKLNFREMFGALVGYMSEQPDLQYHLIIGTDSLLSD
                     KTCFVTAVIVHRVGHGGRYFYRKQFNRKIESLRQRILFETAMSLDLAGQLSGEFARNG
                     HSELPLEIHLDVGPNGDTKRIIREVVGMVTGSGFTAVTKPDAYGASKVADKHSK"
     gene            complement(4722..6206)
                     /locus_tag="AC159B_04400"
     CDS             complement(4722..6206)
                     /locus_tag="AC159B_04400"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HIR3377020.1"
                     /translation="MGLEIVGSRVLAPFFGSSVYVWGSLISIFLAALSLGYYFGGVAA
                     DRWPRTGLLAAMLTVAGALILLLPVVGRPILEAFSLWDLGPRLSPLLASVVLFALPSV
                     LMGATSPFAIKLAATDLATVGNTAGILYAISTAGSIVGTLLTAFVLIPAMGVRAILYT
                     LGASLIVFSGLLAFKSARSRVLRPVVTTALALLLVSGPALGQGDLQWLVFEKDTVYHN
                     IRVYEDVNWRLLRFDRSLQGGMLLKNHLVSPHRYPDFAHLAWLFNPDIKRVLMVGLGA
                     GTISKRILSDYPQASVESVELDPVVVDVAKRFFQVTEEPRHRIIVQDGRQYIRRTQGG
                     YDLIVMDAYNAEGLPFHLATREFLQLTKSKLAPGGVIAAHIVGFLEGSNSKLFRALFK
                     TYASEFSGLYPFAVSVSVRRDEEEVRSIILIATSKGGVAKRDMIGILGRLRREKKASP
                     VLQANYLDDLYQKPILTTDVPIITDDYPWRVDTLPVWGWEPERR"
     gene            complement(6350..7612)
                     /gene="serS"
                     /locus_tag="AC159B_04405"
     CDS             complement(6350..7612)
                     /gene="serS"
                     /locus_tag="AC159B_04405"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HIR3377021.1"
                     /translation="MLDLKLIRDNPDLVRAGVRKKHRDAAVVDRVLDVDRRRRELVQE
                     VERLRAEQNKASGEITKLTGEARERRIAGMREVSTRMKTLEPELKQLEEELDRAMLEL
                     PNLPHDSVPPGKEASENVPLRHWGKPPQFVFQPLDHMEIGNRLGIIDMERGAKVSGSR
                     FYYLKGAGVLLEQALMRLGLDLLMAEGFTPVVTPFLVRPEIIIGAWGGAQLDSQQVYR
                     MEGEDLALIGTSEQTLAGMYKDESFTEDQLPLRLAGMSWNFRREAGSHGRDVRGLYRV
                     HQFDKLEMFAFTTPEQSWSEHERLVAIEERFMQRLGLHHRVVALCGGETSVPAAKTYD
                     LETWMPGRGEFGETQSCSNCTDFQSRRLGIRIRRSKGTVYAHLLNGTLVATSRGLIAV
                     LENYQQKDGSVQIPDALVPYMNGMTVIK"
     gene            complement(7618..8031)
                     /locus_tag="AC159B_04410"
     CDS             complement(7618..8031)
                     /locus_tag="AC159B_04410"
                     /inference="COORDINATES: protein motif:HMM:NF014058.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0072669 - tRNA-splicing ligase complex
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006388 - tRNA splicing, via
                     endonucleolytic cleavage and ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HIR3377022.1"
                     /translation="MKPFEVIEHTADVGIIAYGRSLEELFENAAAGMMHFLITSSSVQ
                     KKVRRTMTVTAGSRDALLINWLNELLVVLHADGFIPAEFDIRELGETQLVAEIAGEGV
                     DTARHAFRLDVKAATYHAFEIGKNDYWHARIIFDV"
     gene            complement(8042..8935)
                     /locus_tag="AC159B_04415"
     CDS             complement(8042..8935)
                     /locus_tag="AC159B_04415"
                     /inference="COORDINATES: protein motif:HMM:NF040786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HIR3377023.1"
                     /translation="MNLHQLRIFYTVARLGSFSRAAEELRISQPSVSIQVADLERSLR
                     VDLFEQLGKRIYLTDAGRVLEDYARRILNLVEEANSALAEVAGEFRGRLTIGASTTPG
                     TYVLPRVIGAFQEKYPNVTVTLDIANTRRIQERILRNELDLGIVGWEVSSHNLEVLPL
                     LEDELVLVVPPGHQLAQAETVQAKVLRDQRVIMRERGSGTREAAEAALREAGVVLSPA
                     MELGSNEAIKEAVAAGLGVTILSTLAVAPEVAARRLVVVPMDDLSIRRSFRVVYHRDK
                     RMGKALRTFIDMLQSSVQTPA"
     gene            9034..9774
                     /gene="selD"
                     /locus_tag="AC159B_04420"
                     /pseudo
     CDS             9034..9774
                     /gene="selD"
                     /locus_tag="AC159B_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810477.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
BASE COUNT         2111 a         2992 c         2843 g         1828 t
ORIGIN      
        1 caaatttatc cgcagcatgg atgatatcaa gtaacaaagt ccgattcttc taccttgtcc
       61 gctatgttcg ctttgttcgc tttgttcgcg aagtcctgcc taaatatccc agcggtagat
      121 tgttccaaag accgccacga cattattcaa cgcatggatg acgatggccg ggaccaatga
      181 acctgtgcgc tgaaatgtgt acgcgacaac cactcccaga acgaagatcg gcaagaagtg
      241 cacgatctcc agatgcacca ccccgaaaaa cgctgccgag gccagggccg cggtaagcac
      301 accccatcgc gcgcgcaggc cgcggtagac gaaacctcgg aaaaagatct cttctgccac
      361 gggcacgacg acacacagta ccaggagtac ccagatcagc tccgacgccg tgactcccga
      421 cgtcaggatt tcggttaccg gatttctcat gtgttctgct tcggccatct gcttggccct
      481 gtcacgcccg atgacggcgc cgacgagtgc aatcgatacc tcttccacgc cgacgctgat
      541 gaagaccatc gccgcggcga gcaaaacgcc gacacccgcc agcgcgggta accggtccgc
      601 gcgcaggcct aacagatccg ccgactgatg gtagcgtcga gtgacgacat acagcgacag
      661 cgcggcaagc agcgcgctct gtacaacggc gtagatcgaa aactctcgaa attctgacgc
      721 ctcgccgatt cccagcgacg cggcaattgc gggcaatgtc aggccgaaga ctagcgcgat
      781 gccgacaatc tcccccagtc cccacattgc cggccgagag tcaaatggaa tggggttgtc
      841 gcaatgcagg caggtcgccc tgtgactcaa tgtcgccgag ccgcacgccg gacaccgcgc
      901 gaccccgccc ggcggccggg caaacagcag gtagaatggc aggaccagga cgagaagcag
      961 gagcgtgccg atcgcccaca acagcggctc attgcggcgc cggccgaacc gggcccggct
     1021 gtcacgatac acccacaacg cgccggcgat cgacgccaga gccgctccga tgagaaatac
     1081 aaagacgctc tcaggaattt cttgcatctt ccaatactta tccctttatc cccttatccc
     1141 gcttttgtca tcacggcaag gatagcgttt gaacgcggcg aatcagcacc cggacaaacg
     1201 atttcgaatt tcggattgac gattgcggat tgtcatgctt gtgagtttcc caattcgcaa
     1261 tccacaattc gaaatccgca attgatttgc ggaggttctt gatgtcccac gtgtcgttct
     1321 accgcaagtg gcgccccaag tcgtttgacg aaatcatcgg ccaggaacgg gtcacacgaa
     1381 cgcttcagaa cgcgattcgt gcgaaccgcg tcgtccacgc gtatcttttc gcgggtcatc
     1441 gcgggactgg aaagactacg accgcacgca ttcttgcgaa agcgctgaat tgcatccagg
     1501 gacctacgcc gactcctgat aacacatgcc ccaactgtga agcgatcacg gcgggctatt
     1561 ccgtggatgt catcgagatc gacgcggcga gcaaccgcgg catcgaggag atccgcgagt
     1621 tgcgtgaccg gatccggctg gccccgaccg aagggcggta caaagtctac attatcgatg
     1681 aagcgcacat gcttaccact gaagcggcca acgcgcttct caagacactc gaggagccgc
     1741 ccgagcatgc cgtgctcatc ctcgtcacga ccgaaccgca ccgtcttccc cagaccatcc
     1801 tctcccgctg tcaacgcttt gactttcggc gcgtctccca aaaggagctc attgccaggc
     1861 tcaggcacat tgcatccacc gaggggttcg aaatcgagga caaagcgctc actattatcg
     1921 cgggcagcgc cgacggctcc gtccgagacg cggaatctgt gctcgatcag ctcgcctcat
     1981 acgccgatgg ccccattgcc gctcgagatg tcctcatggt cttgggcatc ctcgaggaag
     2041 aaatcgccct gaagttcgct gatgcgatca ttgaaaggga tgtcatcgca tctctgggcc
     2101 tggtgaacca ggtcgtgggg gaggggaagg acccccggca gatcacccgg acgatcatgg
     2161 accactttcg agatcttatg gtggcaaaga ccgcggataa tcccaacgag ctcctggaca
     2221 gcacggagac gcgtcttcag atgctgctct ctcaggctac tcgggcccca gtcgaggaca
     2281 ttctgcgagc cctgaatacc ctcgttgcaa cagaggcaga agggcgctgg agtcctcaac
     2341 cccgattgtt gctcgaaatc gcgctcatcc gcctatgccg gcccgagatg gatcctacgc
     2401 tcgaggggct acggacgcga gtgcaggccc ttgagcagcg gctcgtcggc gaatcaccat
     2461 cgcccaaacc ggccggctcg aagcctgcct tggacccgag cgcggcgagt ggttcggggc
     2521 atgtcctgaa cccgagcggc gcgaatggtt cggggcatgt cctgaaccca agcggcgcga
     2581 gtggttcggg gcggacggcg gcgccgaccg gcgtcgagcc catcagaacg caaaaggcgc
     2641 ccgtacgcga cgagatgaag gccgaagaag gagatggagt cacgatcgtg atcggcgacg
     2701 tgcgccgggc gtgggcccgc gtactcgagg aggtgaagcg gacaaaaatg ttctgccacg
     2761 ccctgctgat tgagggtacg ccggttgaat tgaacggctc cacccttgtt gtgagcttgc
     2821 ggactggctt caacttccac gtcgagaatc tgcacaggcc cgagaaccgc accgtcgtag
     2881 aaggcgcgct ccagcgcgtc ttcaaccacc acctgcgcct tgagtgtacg attcatagcg
     2941 aagacacacg gccggctgcg cggccttccg ttgaagaccc tgtggtcaac gcagccatgc
     3001 aactcttcgg cgctcaggtg gtcgacatta agcctgtacc ctgactatgc gcgacatcgg
     3061 caagatgatg aagcaagttc agaagttgca gcaagacgtc gcgcgcgtgc aggctgaact
     3121 gaaggacgag cgagtcgaag cgacggccgg cggtggggtc gtgaaagccg tcgccaatgg
     3181 tcatggcgag atgcaggaaa tcattattga tccgtcggtc gtcgatccgg gcgacgtcgg
     3241 gatgttgcaa gacctcgtcc tcgccgcagt caacgaggcc caacgatctg ccaagcagcg
     3301 cgctgaagag aagatgcgcg ctatcaccgg cggagtggga ctaccgcctg gactgatcta
     3361 atgcgggata ttgggagaag gggataaagg gataaggtca ctgcgctgaa ttgagtgatg
     3421 cttcttccgg cgcagtttgc agtcacctta tccccttatc cctccatccc gttatcccgt
     3481 agttgtgttt ccaaggacat gtacgccgag cccctagccc gtctaatcga agaactctcc
     3541 aagatgccga ccgttggacc caagacggct caacggctgg cattctacat cctgcgcatg
     3601 acgaaagagg atgcggagcg gcttagtcag gccatcctgg acgccaaagc caggattcac
     3661 tactgcggca tttgctttca catcaccgac gtggacccgt gcgcgatctg cgcgaacccg
     3721 gcccgcagcc ggtccatgat ctgcgtggtc gaagaccccc gcgatgtcct ggcgatggaa
     3781 cggacgcgtg aatatcgggg gctgtaccac gtccttcacg gagcaatctc cccgcttgat
     3841 ggggttggtc ccgacgacct gaagatcaag gagctcctgg atcgggtcaa aaccggggag
     3901 gtgcaggagg tcatcgttgc gaccaatccg cgggtggaag gcgaagcgac agccatttac
     3961 ctggcgaaga tcattaagcc gttgggaatg aaggttaccc gtatcgccca cggcgtgccg
     4021 gtcggcgccg atctcgagta tgcagacgaa gtcaccctcg ccagagcgtt ggagggccgg
     4081 agggagatct aacagggaat ccagagactg ccacgccggg ggcgggggca cggacgttgt
     4141 ggaacaaggc gtgacccacg tatactagag acttgtatgg agttcgtcag tccgaccgtc
     4201 ggcaaactca atttccgcga gatgttcggg gcgctggtcg gctacatgag cgagcagccc
     4261 gacctgcaat accacctgat catcggcacc gactcgctcc tgagcgacaa gacctgcttt
     4321 gtcactgcgg taatcgtgca ccgggtcgga cacggcgggc ggtacttcta tcgcaaacag
     4381 ttcaaccgca agatcgaaag cctccgccag cgcatcttgt ttgaaaccgc catgagtctg
     4441 gatctcgccg gacaactttc gggcgaattt gcacgtaacg gccattcaga gttgccgtta
     4501 gagattcacc tagacgttgg gccgaacggc gacaccaagc ggatcatccg cgaggttgtg
     4561 ggcatggtga ccggctcagg ctttactgcg gtgaccaagc cggacgcgta cggggcctcg
     4621 aaggtagcgg ataaacactc aaaataacgg gaacagggaa cggcgaacgg taactacagg
     4681 tcgttaaccg ttcccagttc cccgctcccc gttcccggca gttagcgtcg ttcaggttcc
     4741 catccccaca ccggcaacgt gtcgacgcgc cacggatagt cgtccgtgat gatcggaacg
     4801 tcggtcgtga gaatgggctt ctgatacaaa tcgtcgaggt agttcgcctg cagcacaggc
     4861 gacgcctttt tctcccgccg taggcgccca aggatcccga tcatatccct cttcgcgacg
     4921 ccgcctttgg aagtggcgat cagaatgatg cttcttactt cctcttcatc ccggcggacg
     4981 ctcacgctta cagcgaacgg atacagtccg gagaactcac tggcataggt cttgaagagg
     5041 gctcgaaaga gcttgctgtt ggacccctcc aggaatccga cgatgtgggc ggcgatcaca
     5101 ccacccggcg cgagtttgct ctttgtgagc tgaagaaact cccgagtagc cagatgaaag
     5161 gggaggccct ctgcgttgta cgcgtccatg acgatcaggt cataaccacc ctgagtacgc
     5221 cggatgtatt gccggccgtc ttgcactatg attcggtgcc gcggctcctc cgtgacctgg
     5281 aaaaatcgct tcgcgacgtc cacgacgacg gggtcgagct ccacagattc caccgatgcc
     5341 tgaggatagt cgctaaggat ccgcttggag atcgtccctg ctcccagccc caccatcagc
     5401 acccgtttga tgtcggggtt gaatagccag gcgaggtgtg cgaagtcagg atagcgatgc
     5461 ggactcacca ggtggttctt cagaagcatg ccgccttgca acgaacggtc aaacctcaag
     5521 agacgccagt tgacgtcctc gtagactcgg atgttgtgat agactgtgtc cttttcaaaa
     5581 accaaccatt gtaaatcgcc ctgcccaagc gcgggtcccg agaccagcag gagcgccagc
     5641 gccgtcgtta caaccggccg cagcacacgc gaacgagcgg atttaaacgc gaggagtcct
     5701 gaaaagacga taagagacgc acccagcgtg tagagaattg cccggacgcc catggcaggg
     5761 atcaagacaa aggcggtcag cagcgtaccc acgatactgc cggccgtgga aattgcatac
     5821 aggatccccg cggtattgcc gaccgtggcg agatccgtgg cagcgagctt gatcgcgaac
     5881 ggcgatgtcg cacccatgag aacgctggga agagcgaaca acacgaccga cgcgagcaga
     5941 ggacttaacc gcgggccgag atcccaaagc gagaacgcct cgaggattgg gcgtccgacg
     6001 accggcaaca ggagaatcag cgcgccagcg acggtcagca tggcagcgag caaccctgtt
     6061 cgcggccagc gatcggccgc cacacccccg aagtaataac ccaggctcaa cgccgccagg
     6121 aaaatgctga tcaggctgcc ccagacataa acggaactgc cgaaaaacgg cgctagtacg
     6181 cggctaccaa caatctcaag tcccatcaga acggcacccg aaccgaatac gacaactcgt
     6241 aaagtcacaa gagccccctc agtcgaactg cggtcgtagg tcgtgggtca taggtcgtag
     6301 gcaaatctgt acgcaggttc cacgaccgac gaccgacgac ccacgaccgt cattttatga
     6361 ccgtcattcc attcatatag ggcacaagcg cgtccggaat ctgcacgctt ccatctttct
     6421 gctggtagtt ctcgagcact gcgatcagtc cgcggctggt cgcgacgagg gtcccgttca
     6481 acaagtgcgc gtacacggtt cccttcgacc gcctgatccg gatacccaac cggcgtgact
     6541 gaaaatcggt gcagttgctg cacgactgcg tttccccgaa ttcgcctcga cccggcatcc
     6601 acgtctcgag gtcataggtt ttagccgcgg gaacgcttgt ctcgccgcca caaagcgcga
     6661 cgactcggtg gtgcagccca aggcgttgca tgaaacgctc ctcaatcgcc acgagacgtt
     6721 cgtgctcgct ccaggactgc tccggtgtgg tgaacgcaaa catctcgagc ttatcaaatt
     6781 gatgcacgcg atacagacct cgcacgtcgc ggccgtggct tccagcctcc cgccgaaagt
     6841 tccacgacat gccagccagg cgcagcggga gctggtcttc ggtaaatgat tcatccttat
     6901 acatccctgc cagtgtttgc tcagacgtgc cgatcagggc cagatcctca ccttccatac
     6961 gatagacctg ctggctgtcg agctgagccc caccccaggc gccgatgatg atttccggcc
     7021 ggaccaagaa aggcgtaact acaggggtaa agccttccgc catcagcaga tcgagtccca
     7081 acctcatcag ggcctgctcc agcagcacac ctgcgccttt tagataatag aatcgcgaac
     7141 ccgacacttt cgcgcctcgc tccatatcga tgatcccgag gcggttcccg atctccatgt
     7201 gatcgagcgg ctgaaagaca aactgcggcg gcttgcccca atgccgaagc ggaacgttct
     7261 ccgaagcctc cttgcctggc gggacgctgt catgcgggag attcgggagt tcgagcattg
     7321 cgcggtccag ttcttcttcc agttgcttga gttctggctc gagcgtcttc atgcgtgtgg
     7381 agacctctcg catgccggcg atccggcgtt cgcgggcttc accggtgagc tttgtaatct
     7441 ccccagacgc cttgttttgc tctgcacgca gacgttcgac ttcctgcacc agctctcgcc
     7501 ggcgacggtc aacgtccagc acgcggtcga cgaccgccgc gtcgcggtgc tttttgcgca
     7561 cgcccgcgcg cacaagatct gggttatcgc gaatgagctt caggtccagc atgttgtcta
     7621 gacgtcaaag atgattcgtg catgccagta gtcgttcttg ccgatctcga aagcatgata
     7681 ggtggcggcc ttcacgtcca gccggaaggc atgccgcgcc gtgtcgaccc cctcgcctgc
     7741 gatctcagct accagctgcg tctcgcccag ctcgcggata tcgaactctg cggggatgaa
     7801 tccgtcggcg tgaagcacaa ccaagagctc gttcagccag ttgatcaaca gggcgtcccg
     7861 agaaccggcc gtaacagtca ttgttctgcg aacctttttc tgcacgctcg aggacgttat
     7921 aagaaagtgc atcatacccg cggccgcgtt ttcaaagagc tcttccagtg agcggccgta
     7981 agcgatgata ccaacatcgg cggtgtgctc aatgacctca aacggtttca acaagaagcg
     8041 gctaggcagg tgtctgaacg gacgactgaa gcatgtcgat gaacgtccgt agggctttgc
     8101 ccatgcgctt gtcgcgatga tagaccacgc ggaacgatcg gcggattgat aagtcatcca
     8161 tcggcaccac gacgaggcgg cgtgccgcca cttccggggc tacggcgagc gtggaaagga
     8221 tcgtcacgcc gagtccggcg gccacggcct ccttgatcgc ttcattgctg cccagttcca
     8281 tggcgggcga cagaaccacg ccggcttcac gcagcgcggc ctcagcggct tcccgggtac
     8341 ctgatccccg ctcccgcatg atcacccgct ggtcccggag cacctttgcc tgcacagtct
     8401 cggcctgagc gagttgatgt ccgggcggca cgacaagcac gagctcgtct tcgagcagcg
     8461 gcagcacttc gagattgtga gaactgacct cccacccgac gatacctaga tcgagctcgt
     8521 ttcgcaggat ccgctcctga atgcggcggg tgttggcgat atccagcgtc acggtgacgt
     8581 ttggatactt ttcctgaaac gcgccgatca ctcgcggcaa gacgtaggta cccggtgtag
     8641 tgctggcccc gatcgtcagc cgccctcgaa actcgccggc gacctccgcc agcgcagagt
     8701 tggcttcctc gaccagattg aggatccgcc gcgcgtagtc ctcgagcacg cggcccgcgt
     8761 cagtcaggta gatgcgcttg ccgagctgct cgaacaggtc cactctcagg gatcgttcca
     8821 ggtcggccac ctgaatgctg acagacggtt ggctgatcct gagctcctcg gcggctcgtg
     8881 agaagcttcc caaccgggca acagtgtaaa agattcgcaa ctgatggagg ttcacttcgc
     8941 aagacttcgg gatgtgaacg gagtaaccat tcaccattca cgagggaggg aggtgaggtc
     9001 gtgacggatc gagagcgcat taggctcacc tcgatggtcg cctgcgcagg gtgagcatcg
     9061 aaactgagtc ctgaggacct ggcgcaggtc ctgcgtcagc taccacaatc cagcgacccc
     9121 aacctcctcg tggggaccgg gaccatcgat gatgcggcgg tgtatcgcct caccgacgag
     9181 atggccattg tgcaaaccgt cgacgtgttt acgccggttg tggacgatcc atatcactac
     9241 ggggccattt ccgcggcaaa tgcgctctcg gacatctacg cgatgggcgc aaaaccattg
     9301 ctcgcactga acatcgtcgg gttcccccgt gggaaactcc cgatgtcggt gctcgaacag
     9361 attctgcgcg gtggatcgga caagacaatg gaggcagggg ccatcatcgc cggcggccac
     9421 agcattgacg atccagagcc caaatatggc ctggcggtga cgggcgtcgt gcgtcccgac
     9481 cgggtcgtga agaacgtcgg cgccttgccc ggcgaccagc tcgtgctgac gaagccgctg
     9541 ggaataggga tcatcacgac cggcatcaag caagaaaaga gctccacggc cgctatcgac
     9601 gaggtgatcg atgatatgga tcgcctgaac agcgatgcat cataggcgat ggtcgaagtc
     9661 ggcgtcaatg ccgcgaccga cgtcacagga ttcggcctcc tcggccacct ctttgagatg
     9721 acctcaggca gtcgtgtcac ggcgcggatg tcgttcgccc gggtgcccgt gctc
//
LOCUS       DBNGSB010000153         4166 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3939924, whole genome shotgun sequence.
ACCESSION   DBNGSB010000153 DBNGSB010000000
VERSION     DBNGSB010000153.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4166)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3939924"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="AC159B_04425"
     CDS             complement(<1..342)
                     /locus_tag="AC159B_04425"
                     /inference="COORDINATES: protein motif:HMM:NF015424.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3377024.1"
                     /translation="MEQMLTTQQAADLLRMHVKSVQSMARRGKIPGVRVGRKWLFSRA
                     TLLSRLGGLALGAPTRLTDLSGRNQLPGQVASISVSGVMGEVRVRVGEHELASIITRA
                     SVKRLGLKVGDE"
     gene            465..842
                     /locus_tag="AC159B_04430"
     CDS             465..842
                     /locus_tag="AC159B_04430"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HIR3377025.1"
                     /translation="MGNSARSQIAEGIFNASPPSGWRASSAGTEPAQRVRPEAISVMR
                     EIGVDISGHRPKHLRDALGSDVSLVIGLCAEEACPVVPGATSEHWPLRDPAGSTDLKV
                     YRELRDELRRRIDDLIVRLSAGS"
     gene            839..1675
                     /locus_tag="AC159B_04435"
     CDS             839..1675
                     /locus_tag="AC159B_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3377026.1"
                     /translation="MTEHRIFGSTGVQVPIVGQGTWEMDQDGVERSVAALRRGLDLGM
                     THIDTAESYGSGEVEEIVGKAITGRRPEVFLVSKVSPGRATRRGTIQACEESLRRLGT
                     DYLDCYLLHWPGRHPLADTFAAFEQLVRDGKSKAWGISNFNEEEVEEAVRIAGTGRIA
                     CNQVLYHLEERAIEHHVIPACEEHNIAVVAYSPFSQGRFRSHRVLEDIARSKGVSARQ
                     VALRFLLRHPGTFTIPKSATMAHVEENAGAADLQLTDGEISRIDQAFPTRKPRRGHLP
                     VL"
     gene            1714..2646
                     /locus_tag="AC159B_04440"
     CDS             1714..2646
                     /locus_tag="AC159B_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3377027.1"
                     /translation="MAVATLVAIVMALWLGRDGLPFNRPSVDSTDVVPNIFGQLVQLA
                     FALFIIRVASRVTPRRSIVDFDERTPARQIARSEMIWLLLYGVVVLFAGGLNGIGMHL
                     HGAIYGPTRAIPPRDVYVWAAYNFLFFAAIPYLAFRKRGYDHRSMFLKSENARGDAIL
                     ILVVLILESAGELFTFPGLFSLTARQLLIGIPLTFVLHLVGTGLPVMVFAYALLFPRY
                     MKVTRSPASAIVCGALTYAGLHLFEYWTVYDSLTNGVLSIAFLLLQFTPPGLVKSYLT
                     WRTGNAWVHLWAYHAVAPHVTVDTPHVVKIFGIR"
     gene            complement(2656..2856)
                     /locus_tag="AC159B_04445"
     CDS             complement(2656..2856)
                     /locus_tag="AC159B_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3377028.1"
                     /translation="MHSAEIAPEQAETDQHRQLSVTLIVRGGPLPPDLILAVTEWTRR
                     GLRAVGLTDIPLQVKQVSHESE"
     gene            complement(3137..3793)
                     /gene="phoU"
                     /locus_tag="AC159B_04450"
     CDS             complement(3137..3793)
                     /gene="phoU"
                     /locus_tag="AC159B_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018035148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HIR3377029.1"
                     /translation="MTREAFERELHTLKDDVVRMGTQAGELIRRAVEALKTRDVALAE
                     QVISEDNAIDALHLDLERRCMRLLATQQPMAGDLRTIASVFAITIDLERMADHAEGIS
                     RAVRRLGSEPLVKPLVDIPKMETHIQEMLRDILEAFRGQNAELAIRAAAKDDAVDQLR
                     NRVFHDLLDIMIKDPQTIHRALELLIVARHLERAADHLTNVGERIVYMVTGEMRELNV
                     "
     gene            complement(3793..>4166)
                     /locus_tag="AC159B_04455"
     CDS             complement(3793..>4166)
                     /locus_tag="AC159B_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HIR3377030.1"
                     /translation="EAAALWDEVKDRLQKPGTSLSGGQQQRLCIARALAVSPEVLLMD
                     EPASALDPASTLHIEELARELAKDITIVIVTHNMQQAARVSVETGFFLDGELIEFGLT
                     NDIFTRPKERRTEDYITGRFG"
BASE COUNT          787 a         1289 c         1239 g          851 t
ORIGIN      
        1 ctcgtccccg actttcaaac ccagccgttt caccgaggcc cgcgtgatga tcgaggcgag
       61 ctcatgctcg cccacccgca ctctcacctc acccataacc ccggaaacag aaatggaagc
      121 gacctgcccc ggtaactggt tacgcccgct taagtcagtc agacgagtcg gcgctccaag
      181 cgcgagaccg ccgagtctgg acagaagagt cgctcgagag aacaaccact tgcgcccgac
      241 gcggacgccg gggatcttcc cgcgccgggc catggactgg actgacttca cgtgcatgcg
      301 cagcagatct gctgcctgct gggttgtcag catttgctcc atgattacct tatattacct
      361 aaatatccct acttgggcaa ccgccagaac ataagggaaa ggggggaggc tgcgacccgg
      421 agagagaaag tgatgctgcc gtgaagcgcg tgttatttgt ctgaatcggt aatagcgccc
      481 gatcccagat agcggagggg atcttcaatg catccccccc atcagggtgg cgggccagct
      541 ccgcgggtac ggagccggcg caacgcgtcc gaccggaggc catctccgtc atgagggaga
      601 tcggagtcga catctccggc caccggccga aacatcttcg cgatgctctc ggatctgacg
      661 tctctcttgt gatcggccta tgcgccgagg aggcgtgccc cgtcgtacct ggcgcgacga
      721 gtgaacactg gccgctgcgc gatcccgccg gcagcacaga cctcaaggtt tatcgcgagc
      781 tacgggacga gctccgcaga cgaattgacg atttgatcgt caggttgagt gcgggatcgt
      841 gacggagcac cggatcttcg gctcgacggg cgtccaggtc cctatcgttg gtcaaggcac
      901 ctgggagatg gatcaagatg gcgtcgagcg gtcagtcgcc gccctgcgcc gtggcctcga
      961 cctcgggatg acgcatatcg acacagccga gagttatggc agtggtgaag ttgaagagat
     1021 cgtcgggaaa gccatcaccg gccggcggcc cgaggtcttt ctcgtatcaa aagttagtcc
     1081 cggacgcgcg acacgccgcg gcacgattca ggcatgcgag gaaagcctgc gacgcttagg
     1141 taccgactat ctggactgct acctgctcca ctggcccgga cgccatccgc tcgccgacac
     1201 gttcgccgcc ttcgagcaac ttgtccgcga tggtaagagc aaagcctggg gcatttcaaa
     1261 cttcaatgaa gaggaagtcg aagaagccgt tcgcatcgcc ggaacaggcc gtatcgcctg
     1321 taaccaggtg ctctatcatc ttgaagaacg ggcgatcgag catcatgtga tcccggcttg
     1381 tgaggaacac aatattgcgg tcgtggcgta cagtccattc agccagggcc gctttcgctc
     1441 tcaccgcgta cttgaggaca ttgcacggtc gaagggtgtg agcgcaagac aggtcgcgtt
     1501 gcgattcctg ctccgccacc ccggcacgtt cacgattccc aagtccgcga cgatggctca
     1561 tgtcgaggag aatgccggcg ccgcagatct tcagcttacg gacggcgaga tcagtcggat
     1621 cgatcaagca ttcccgacaa gaaagccacg ccgcggtcac cttcccgtgc tttgatgccg
     1681 acgcccctgc ttcggaatcg gtcggtacaa ggattggcgg tcgccacgct cgtcgcgatt
     1741 gtcatggcac tgtggctcgg aagagacgga ctgccgttca accgtccctc tgtggactcg
     1801 accgacgtcg ttccgaacat cttcggtcag ctcgtgcagc tcgcattcgc cctctttatc
     1861 atccgcgtag catcccgtgt gaccccccga cggagcattg ttgatttcga cgagcggact
     1921 ccggccaggc agatcgcgag aagcgagatg atctggctac tgctgtacgg agtcgtggtg
     1981 ctgtttgccg gtggcctgaa cgggatcggc atgcatctcc acggcgcgat ctacgggccc
     2041 acccgagcga tcccgcccag ggacgtctac gtgtgggctg cgtacaattt tctgttcttc
     2101 gccgctatcc cttacctggc gttccgcaaa cgaggatatg accatagatc gatgttcctg
     2161 aagtcggaga atgctagggg cgatgcgatc ctcatcctcg tcgtgctcat ccttgaatcc
     2221 gcaggagagc tgttcacatt tcccggccta ttctctctca cggcccggca gctcttgatc
     2281 ggaatcccgc tgacattcgt gctgcatctc gtaggcaccg gcctgccggt catggtcttc
     2341 gcgtatgcgc tcctcttccc aaggtacatg aaggtgacac ggtccccagc ctcagcgatc
     2401 gtctgcggag ctctgaccta cgcggggcta cacctattcg aatactggac ggtctatgac
     2461 tcgttgacca atggcgtgct gtcgatcgcg ttccttctgc tccagttcac gccgccggga
     2521 ttggtgaagt cttacctgac gtggagaacc gggaacgcgt gggtgcacct ttgggcctat
     2581 catgccgtcg cgccccacgt caccgtggat acgcctcacg tcgtgaagat atttgggatt
     2641 cgctagggcg gtgagctact ccgactcgtg agaaacctgc ttcacctgaa gcgggatgtc
     2701 cgtgagtccg acagcacgca aaccacgccg cgtccactct gtcacggcca ggatcagatc
     2761 aggcggaagc ggtccgcccc ggacgatcag cgtgacgctc agctgcctgt gctgatcagt
     2821 ttcggcttgt tcaggagcaa tttccgcgct atgcattgtc acgatggagc ctcccaacgc
     2881 cgcgatcgcc gccagccgag ggagggcagg ctggcggcgc cgcacggatc aaccgtcctt
     2941 tgagacgcct gctgcgcttc gatccagccg ggctccccat catcttggtc ctgcagccgg
     3001 accgtacagc aagcgctcgg tcgagacggt aatcacccac cctttgcccc gcactttcgc
     3061 ccttactcta ctcggtagat gtgagattcg tgcgaaggtg gtatcaagcg tgagtgaaga
     3121 tcgtgagaaa tgttggtcag acgttgagct cgcgcatctc gccggtcacc atgtagacga
     3181 tgcgctcgcc cacgttggtg agatggtccg cagcgcgctc caggtggcgc gcgacaatga
     3241 gcagttcgag cgcacggtgg attgtctgcg gatctttgat catgatgtcc agcagatcgt
     3301 ggaagacgcg gtttcggagc tgatcgacag cgtcgtcctt cgccgccgcc ctaatcgcga
     3361 gctcagcatt ctggccgcgg aaggcctcga gtatgtcccg cagcatctcc tgaatgtgag
     3421 tctccatctt gggtatgtcg acaagaggct tgaccagggg ctcgctgcca agccgcctca
     3481 ccgcccgaga gataccttcg gcgtggtcgg ccatccgctc gaggtcgatg gtaatcgcaa
     3541 acaccgacgc aatggtgcgg agatctccgg ccatcggctg ctgggtggcg agcaggcgca
     3601 tgcaccttcg ctcgaggtcc agatgcaggg cgtcgatggc gttgtcctcg gagatgacct
     3661 gttccgccag cgcgacatca cgcgtcttca atgcttccac ggcacggcgg atcagctccc
     3721 ccgcctgcgt gcccatgcgc acgacatcgt ctttgagcgt gtggagctcg cgctcaaatg
     3781 cttcgcgggt cactagccga accgtcccgt gatgtaatcc tccgtcctcc gctctttcgg
     3841 tctggtgaag atgtcgtttg tgagtccgaa ctcaatgagc tcaccgtcga gaaaaaagcc
     3901 cgtctcgacg gagacgcgag ccgcctgctg catgttatgc gtgacgatca cgattgtgat
     3961 gtctttggct agttcacgag cgagttcctc gatatgcaag gtgctcgccg gatccagcgc
     4021 agaggccggt tcgtccatca agagaacctc cggcgagacg gccagcgccc gcgcgatgca
     4081 caggcgctgc tgctggcctc ccgagagact ggtgccgggt ttctgcaggc ggtcttttac
     4141 ctcatcccag agtgccgcag cttcca
//
LOCUS       DBNGSB010000154         3455 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2406704, whole genome shotgun sequence.
ACCESSION   DBNGSB010000154 DBNGSB010000000
VERSION     DBNGSB010000154.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2406704"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(231..869)
                     /gene="sigH"
                     /locus_tag="AC159B_04460"
     CDS             complement(231..869)
                     /gene="sigH"
                     /locus_tag="AC159B_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HIR3377031.1"
                     /translation="MALARREAPSYQDMLDEDLVASAKSGDDQASEYLINKYRNFVRV
                     KAKAYFLIGADREDIIQEGMIGLYKAIRDFRADKLSSFRAFAELCITRQIITAIKTAT
                     RQKHIPLNSYISLNKPIYDEDSDRTLLDVISSIKVSDPEELVINQEASATMRERIRKN
                     LSELECKVLTAYLEGKSYQEMANELNRHVKSIDNALQRVKRKLERNLEGEED"
     gene            complement(994..2328)
                     /locus_tag="AC159B_04465"
     CDS             complement(994..2328)
                     /locus_tag="AC159B_04465"
                     /inference="COORDINATES: protein motif:HMM:NF016202.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HIR3377032.1"
                     /translation="MISLLAGIFGLVAGVLHDTQAYSDRVLPGISIAGAQVGGLTPDQ
                     AQDVAEAAAAAQLSRTVTIRIQDERFALTYGELGLISHVGEAVRDAYGIGRDEPWQQL
                     VFTRMQLAWHPMNVPIRYSWNRGAVNKILAPLAAEINIEPQNAAVTVQNGKVVVTRES
                     RSGRTLDLAATGARIRSALGTGRDEVAAVMRVRDPEFTSDAARALVAPLGQFTTKVPG
                     TENRVHNVALAASYVSGTIVGPGGVFSYNQVVGPRTGARGFREAPILIDNELVQGDGG
                     GVCQVSSTLFNVAVLADFQILARANHSRPVAYLPLGRDATVSYGAYDLQFKNTTGHHV
                     LIWAWVTDRRLTVMAFGTPEPGKDVAIEVARSGDIAPPEGTRTKHDPELAEGKIVERE
                     ALPGYRAKTYRVVTVNGEVVRRELIGSSIYNAVARTIKIGTKKATQPEDDHS"
     gene            complement(2526..3275)
                     /gene="rlmB"
                     /locus_tag="AC159B_04470"
     CDS             complement(2526..3275)
                     /gene="rlmB"
                     /locus_tag="AC159B_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HIR3377033.1"
                     /translation="MGDEELIYGRQAVLEALRASRPLRKILVSRGSRPSGAVRAILDH
                     ARQQDIAVQFVDQRRLDELVRGRHQGIVAMASAKSLASIEQILDAARATGRLPLVLVL
                     DGIEDPANLGAILRTADAAGAHGVVIPKHRAVGLTSTVARTSAGAIEHVPVAQVTNLV
                     RALEDLKEAGLWVVGADPKATQNLYEVDLTKALAVVIGGEGKGLGRLVLEHCDLLVRL
                     PMQGKISSLNAAVAIGIVLFEILRQQREARP"
     gene            complement(3280..3450)
                     /locus_tag="AC159B_04475"
     CDS             complement(3280..3450)
                     /locus_tag="AC159B_04475"
                     /inference="COORDINATES: protein motif:HMM:NF049034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysS/YqeB C-terminal domain-containing protein"
                     /protein_id="HIR3377034.1"
                     /translation="MSSILDVREKARASKDFSAADQIRKLLAEAGIIVEDLPTGPRWR
                     VVSAAGPAPRGA"
BASE COUNT          610 a         1084 c         1039 g          722 t
ORIGIN      
        1 gatgaccccg acccggagtt tactcatcat tttactcgct ctctctagcc gcactcagag
       61 ggcggagcct ccgaaactgg cctgcgccgg ggaggtcgca gcctgagggg tccccgtatc
      121 agtttcagtc ggggagaggc tgtgacctcc ccgaaagcag gccaagaccg aaatagaaaa
      181 gggataaggt tgaatcctta tccctcagtg cagcgcggcc tgcggctcgc ctagtcttct
      241 tcgccctcca aatttctctc aagctttcgc ttcacccgct gcagggcatt gtcgatcgac
      301 ttcacatgcc ggttcaattc attcgccatt tcctgatagg atttgccttc gaggtacgcc
      361 gtcagcacct tgcactcgag ctcgctgagg ttcttgcgga tgcgctcccg catcgtcgcg
      421 gaggcttcct gattgataac aagctcttca gggtccgaca ctttgatgct gctgatcaca
      481 tccagcagcg tgcggtccga gtcctcgtcg taaatcggtt tgttcagcga gatgtatgag
      541 ttgagcggga tgtgtttctg tctggttgcg gtcttgatcg ccgtgatgat ctggcgggtg
      601 atgcacagtt ccgcgaacgc ccggaacgag gagagcttgt cggctcggaa gtcccggatc
      661 gctttgtaga ggccgatcat tccttcctgg ataatgtctt cgcgatcggc accgatgagg
      721 aagtacgcct tcgccttcac gcggacgaag ttgcggtact tattgatcag gtattcgctt
      781 gcttgatcgt caccagactt agcagaagca acgaggtctt catcgagcat atcctggtag
      841 gacggagctt cgcgacgagc aagtgccact ctgcagcctc ccagagcctc cctgaacccg
      901 tagcgaagcg aatgatccag ggccaagatt cgacgttaat tatacgaacg gcctatggaa
      961 gcgtcaacag aattgtgcgc accgcggtgg gacttacgag tggtcgtcct caggctgcgt
     1021 ggccttcttg gttccgatct tgatcgtgcg cgcgaccgca ttgtagatgc tgcttccgat
     1081 gagctctcgc cggacaactt ctccgtttac ggtgacgacg cggtacgtct tcgcgcgata
     1141 accgggcagc gcttcccgct cgacgatctt gccctctgcg agttcgggat cgtgcttggt
     1201 gcgcgtgccc tcgggcggtg cgatgtcgcc cgaccgtgcg acctcgatgg ccacgtcttt
     1261 tccgggctcc ggcgtgccga atgccatcac cgtcaggcgg cgatccgtga cccatgccca
     1321 gatgagtacg tgatggcccg tcgtgttctt gaactgcaga tcgtacgcgc cgtaactcac
     1381 ggtcgcatcc cggcccagcg ggaggtaggc caccggccgg ctgtggttcg cgcgtgcgag
     1441 gatctggaag tcggcgagga ccgcgacgtt gaagagcgtc gatgacacct ggcatacgcc
     1501 cccaccatcg ccctggacga gctcgttgtc gatgaggatc ggggcctccc tgaaaccgcg
     1561 cgccccggtc cggggaccga cgacctgatt ataggagaag accccgccgg ggccgacaat
     1621 ggtgccgctg acgtagctcg cggcgagtgc gacgttgtgc acccggtttt ccgtgcccgg
     1681 cactttcgtc gtgaattgcc caagcggcgc cacgagggcg cgggctgcat cactcgtaaa
     1741 ctctggatct cttacccgca tcacggccgc gacttcatct ctgccggttc caagcgcgct
     1801 ccgaattcgc gcgccggtcg ccgccaggtc cagcgtccgg ccgctccggc tctctcgcgt
     1861 cacaacgacc ttgccgttct gcacggtcac cgcggcgttc tgaggctcga tgttgatctc
     1921 cgcggcgagc ggagccaaga tcttgttcac ggctccgcgg ttccagctat agcggatcgg
     1981 cacattcatc ggatgccatg cgagctgcat cctggtgaac accagctgct gccacggttc
     2041 gtcgcgcccg atgccgtagg cgtcgcggac cgcctcgccg acgtgcgaga tcaagcccag
     2101 ctctccatac gtcagggcga aacgctcatc ctgaatcctg atggtgactg tccgcgagag
     2161 ttgggccgcc gccgccgctt cagcgacgtc ttgggcctga tcgggagtca gcccgccgac
     2221 ctgcgctccc gcgatcgaaa tgccgggaag aacacgatcc gagtacgcct gggtatcatg
     2281 aagcactccc gcgacgaggc cgaagatccc ggcgagcagg ctgatcaaag cgacgagcga
     2341 gagtgggctg accgcgacat gctagcgccg gccttccttg ccatagaagg ccggctgatc
     2401 ttgcgtaatg acgttggaac aacccgccga gtcctgcatt ccctctgacg gcggcgcggt
     2461 accccctgat cgcgtatacg atttgccgcg cttccgcgct gccgctctgt tctgtttttc
     2521 cccgcttagg gtcgagcctc acgttgttgt cgaaggatct caaacaaaac gatgccgatt
     2581 gccactgcgg cgttgaggct cgatattttt ccctgcatgg gtagacgcac caggagatca
     2641 cagtgttcca gaaccaggcg tcccagtcct tttccctccc ctccaatgac cacggcgagt
     2701 gcttttgtga ggtcaacttc atagaggttt tgcgtagctt ttggatccgc cccgaccacc
     2761 cacaggcccg cttccttgag gtcctccagt gcgcggacca agttcgtgac ttgcgccacg
     2821 gggacgtgtt cgatcgcccc cgcggatgtc cgggccacgg tactcgtgag ccccacggcc
     2881 ctgtgcttgg gaatgaccac gccatgagca cccgcagcat ccgctgtacg caggatcgct
     2941 cccaaattcg cggggtcttc gatgccgtcg aggacgagca cgagcgggag cctgccggtt
     3001 gcacgcgcag cgtcgaggat ctgctcgatc gacgccaggg acttcgcgga cgccatggcg
     3061 acgatgcctt gatggcggcc gcggaccagc tcgtcaaggc ggcgctgatc tacaaactgg
     3121 acggcgatat cttgttgccg cgcgtgatcg aggatggctc gcaccgcgcc ggacggccgg
     3181 gatccacggc tgacgagaat cttgcgcagt ggtcgcgagg cacggagtgc ttcgaggacg
     3241 gcctgtcggc cataaataag ttcctcgtcg cccactctat caagcgccgc gaggcgcggg
     3301 gccggcggcg gagactacgc gccagcgcgg gccggtcggc aaatcttcaa cgatgatgcc
     3361 ggcctcggcc aacaacttac ggatttgatc tgcggcagaa aaatctttgc tcgctcttgc
     3421 tttctcacgt acgtcaagaa tgctcgagat taatg
//
LOCUS       DBNGSB010000155         3384 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_874602, whole genome shotgun sequence.
ACCESSION   DBNGSB010000155 DBNGSB010000000
VERSION     DBNGSB010000155.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_874602"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1228)
                     /locus_tag="AC159B_04480"
     CDS             complement(<1..1228)
                     /locus_tag="AC159B_04480"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3377035.1"
                     /translation="MTPIEATVVWRPTASYVEESRLTQLMRRHSLADYDSLFRRSVED
                     LEWFWNAVNEDLALQWVRPYSKVFDSSAGIPWTKWWIDGRYNYVQNCLDRHLPKFKEK
                     IAIRYEGEEGSTRTLTYGQVLAEVNRLANGLKRLGIRKGDRVGIFMPLTPECVIATLA
                     VSRIGAIYIPIFSGFGASAVATRLADCDAVALITADSFPRRGRPVPMKETADEALRSA
                     STVRHVIVHKRQGTHVSWNTTRDHWWDDVVKSESDDCSTEPTDAEDPFMIIYTSGTSG
                     RPKGTVHVHGGFPIKGAMDMAYGFDFKETDTMFWLTDLGWMMGPWEIIGVLTLGGTML
                     LYDGAVDHPEPDRLWALLERHRITVAGISPTAIRAMMRYGEEPVKRHDLGALRLLGST
                     GEPWNPEPWLWYFNHVG"
     gene            complement(1270..2241)
                     /locus_tag="AC159B_04485"
     CDS             complement(1270..2241)
                     /locus_tag="AC159B_04485"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012913.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HIR3377036.1"
                     /translation="MKRIVVLTTGGTISTRDIDGSGAKPTLRGDDLLREIPGIDQMAE
                     VSVEELAFIPGASMTLDLMRQMSERSTSILARRDVDGLVVTHGTDTLEESAYLVHVTV
                     ESEKPIVFTGAMRNASQIGFDGYRNLSDAVRVAAHDSARGAGTVVVLNEEIHSARWVT
                     KTNGQKEDTFKSPATGPMGVIYGDGPRFLMRPIPRTILPPTLEARVDLIRLCVDGDDK
                     FIRVCTQSGSQGIVLEVFGGGRVNPTSLEAIDQALAKGLAVAATTRCLTGNMWDMYGY
                     PGAFRDLQRRGILFAHDLPGHKARLKLMVALGNALPRDRMSAYLAEV"
     gene            2361..2945
                     /locus_tag="AC159B_04490"
     CDS             2361..2945
                     /locus_tag="AC159B_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3377037.1"
                     /translation="MRPLFIVVLIATFFMAATAIGIAQPERPGDEFKDELKPSEIGDA
                     IVVPGERIGPLRLGMSIAQMIRAMPSGYKRDVFEKENVILYEWRKEGVWVSIDGQSRA
                     IRLISVFGAGAYKTDKGLALLHTESKMESVYGRDYKRYEYPDDRITLVRYVPLGLHFG
                     IANYPSQRAIHGRIFTIGIFTPGNEPPLTKTPSK"
     gene            3101..>3384
                     /locus_tag="AC159B_04495"
     CDS             3101..>3384
                     /locus_tag="AC159B_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3377038.1"
                     /translation="MTTRYFGERIKRSEDPRLLTGRGTYVDDVQPRGCLHAAVLRSPH
                     AYARISGIDISKAQALRGVIAVYTNADLSEAIAGPLPKLIPHPSLIHHKT"
BASE COUNT          650 a         1038 c         1009 g          687 t
ORIGIN      
        1 cgccgacatg gttgaagtac cacagccacg gctctgggtt ccagggttcg cccgtggagc
       61 ccagcaggcg cagcgcaccg agatcgtgcc gtttgacggg ttcctcaccg tagcgcatca
      121 tggcgcggat ggcggtcggc gaaattcccg ccaccgtgat cctgtgccgc tcaagcagcg
      181 cccagaggcg atcgggctct ggatggtcaa ccgcaccatc atagagcagc atcgttcccc
      241 ctagcgtcag gacgccgatg atctcccacg gtcccatcat ccatccgagg tcggtcagcc
      301 agaacatcgt gtcggtttct ttaaagtcga agccgtaggc catgtccatc gcgcccttaa
      361 ttggaaagcc gccgtgtacg tggacggtgc ctttcggccg gcctgaggtg cccgaagtgt
      421 agatgatcat gaatggatct tccgcatctg tcggttcggt tgaacagtca tcggattctg
      481 atttaacgac gtcatcccac caatgatcgc gggttgtatt ccaggacaca tgcgttccct
      541 gacgcttgtg cacgataacg tgccgcaccg tcgaggcaga acgcagcgcc tcatccgccg
      601 tttccttcat cgggaccggg cggccacgcc gcgggaagct gtccgccgtt atgagtgcga
      661 ccgcgtcgca atccgccaat cgcgtcgcga ccgcggacgc gccgaagccg gaaaagatcg
      721 ggatataaat cgcgccaatg cgggaaaccg ccagggtcgc gatcacgcat tccggtgtga
      781 gcggcatgaa gataccgacg cggtcaccct tgcgtattcc cagacgtttg agtccgttgg
      841 ccagccggtt gacctccgcc agcacctggc cgtacgttag ggtgcgcgtg ctgccttctt
      901 caccttcata tcggatcgct attttctcct tgaacttcgg aagatgcctg tcgagacagt
      961 tctgcacgta attgtacctg ccgtcgatcc accatttggt ccacgggatg cctgcggagg
     1021 aatcgaacac cttgctgtac gggcgaaccc actggagcgc gagatcctcg ttcacggcgt
     1081 tccagaacca ctcgaggtct tcgaccgacc ggcggaagag cgagtcgtaa tcggccaaac
     1141 tatgacggcg catgagctgg gtgagccggc tctcctcaac atacgaggca gtggggcgcc
     1201 agacgaccgt cgcctcaata ggcgtcaatg acgacatggg ttgatgagag aattctacga
     1261 cggcccggct cagacttcag ccagataggc gctcattcga tcgcgcggga gtgcgttgcc
     1321 gagcgccacc atgagcttta gccgcgcctt gtgccccggc aggtcgtgcg cgaacaggat
     1381 gccgcgtcgc tgcaggtccc tgaacgcgcc tgggtagccg tacatgtccc acatgttgcc
     1441 ggtaaggcaa cgcgtggtcg ccgcgaccgc gaggcctttt gccagcgctt gatcgatcgc
     1501 ttccagcgaa gtcgggttta cgcgaccgcc cccgaatact tccagcacga tcccttggct
     1561 ccccgattgt gtgcacactc tgatgaactt gtcgtcgcca tcgacacaca gtcgaatgag
     1621 atcgaccctg gcctcgagcg tcgggggaag aatcgtgcga gggatcgggc gcatgaggaa
     1681 cctcggtcca tccccgtaga tcacgcccat cgggcccgtg gccggcgatt tgaacgtgtc
     1741 ttccttctga ccgttggtct tcgtgaccca gcgcgccgag tgaatctcct cgttgagcac
     1801 aaccactgtg cccgccccgc gcgcgctgtc gtgggcggcc acccggacgg cgtcagagag
     1861 attccggtag ccgtcgaacc ctatctgtga cgcgttgcgc atcgcgccgg taaacacgat
     1921 cggtttctcg gattcgaccg tgacatggac gaggtaggcg ctctcctcga gtgtgtcggt
     1981 gccgtgggtc acgacgagcc cgtccacatc tcgtctcgcc agaatcgacg tggatcgctc
     2041 gctcatctgc cgcatcaggt ccagggtcat cgatgcccct ggaatgaacg ccagttcttc
     2101 cacagacact tcggccatct gatcgatgcc gggaatctcg cgcagcaggt catctccgcg
     2161 cagggtcggt ttggcgccgc tgccgtcgat atctcgagtg gagattgtcc cgcctgtggt
     2221 cagcaccacg atgcgtttca ttgcctgcct cctatcattg aaaatgaacg ggcacacagt
     2281 cgttgtgagc ggaaggaaca atctcctttc cttggggaaa ggtgttgagg attgagagag
     2341 gatgtcctgg gaggttcggc atgcgcccgt tgttcatcgt cgttctcatc gccacattct
     2401 tcatggcagc gaccgcgatc gggatcgcgc agcccgaaag gcctggagat gagttcaagg
     2461 acgagttgaa gccgagcgag atcggcgatg ctatcgtcgt gccgggcgag cgcatcggcc
     2521 cgctgcgcct ggggatgagt atcgcgcaga tgattcgagc gatgccatcg ggctacaagc
     2581 gtgatgtctt tgagaaagag aacgtcattc tttatgagtg gcgcaaggag ggcgtgtggg
     2641 tgtcgatcga tgggcaaagc cgtgccatac ggctgatcag cgtgttcggt gcaggcgcgt
     2701 acaagacgga caagggcctc gcgctgctcc acacagagtc aaagatggaa tccgtgtacg
     2761 gaagagatta caaacggtac gagtaccccg acgatcgcat cacactggtg aggtacgttc
     2821 ccttgggcct gcatttcggc atcgcaaatt atccgagcca gcgggcgatt cacggtcgca
     2881 tcttcacgat cgggatcttt acccccggca acgagccgcc actaacgaaa accccctcca
     2941 agtaagatcg cctccgggtg ccaccctcca actttggccg ctcccgagta cctggtactt
     3001 ttttggctga atttcggtac ctgctaccgt tttggctgaa aactgagggg tgaggggttg
     3061 aaggactcgt caccgggcgc ccctaatccc atagtctctc atgacgaccc gttatttcgg
     3121 cgagcggatc aagcgcagcg aggatccgcg actgctaaca gggcggggca cctacgttga
     3181 cgacgtgcag ccccgcgggt gcctccacgc cgccgtcctt cggagccccc acgcgtatgc
     3241 gcggatttcc ggcattgata tttcgaaggc gcaggcgctg cggggcgtca tcgccgtcta
     3301 cacaaacgca gatttgtccg aggcgatcgc cggaccgctc cccaaactca ttccgcaccc
     3361 gtcactgatc catcataaga cgca
//
LOCUS       DBNGSB010000156         2674 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2102355, whole genome shotgun sequence.
ACCESSION   DBNGSB010000156 DBNGSB010000000
VERSION     DBNGSB010000156.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2102355"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(336..1178)
                     /locus_tag="AC159B_04500"
     CDS             complement(336..1178)
                     /locus_tag="AC159B_04500"
                     /EC_number="3.5.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HIR3377039.1"
                     /translation="MNETQDSLLATDEPPPFTVDNENGTSPLLIVADHAGKHFPRRLG
                     QLGLSNAESESHIAWDIGAGAVCCLIGRTLDAVVVRQNYSRLVIDCNRTPGSETSILD
                     LSELTCVPGNIGLSEDHKLARDREIFQPYHDRIAIELDRRREAALPTALISVHSFTPV
                     FNTVARPWHVGVLYNRDRRFARILMELLHREEGLVVGDNEPYSVTDASDYTIPVHGEQ
                     RDLHHVALEIRQGLITDEAGQRMWAALFARLLPHAYQRLTGGPARRLGIKRRTRTTSL
                     QVSK"
     gene            1282..2205
                     /locus_tag="AC159B_04505"
     CDS             1282..2205
                     /locus_tag="AC159B_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020092877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HIR3377040.1"
                     /translation="MTILTVRHITTYQYKQPVSFGEHRMMLRPREGHDQRVLESKLEI
                     TPKPTNLRWIRDVFGNHVAIARFAGRADVLRFESTSCLDHSPADILDMDIEDFARTYP
                     FIYGAEDLPDLVRFVERHSSDPDHQLDQWVRALVRKGGSINTRALLVNLTRTIKQTFT
                     HVARHEKGIQDPLLTLQIGSGSCRDLAMLMIEAVRSLGMAARFVSGYLHVRTDHTEGC
                     ARGGNTHAWVQVYLPGPGWVDFDPASGIVGNHDLVRVAVVRDPRQAIPLHGTWTGFPS
                     DKLGMTVEVNVTAAEAQLASRSARTSSGMAL"
     gene            2323..>2674
                     /locus_tag="AC159B_04510"
     CDS             2323..>2674
                     /locus_tag="AC159B_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HIR3377041.1"
                     /translation="MEIRVGYELIYDCPQPTPMMLVLNIHYSRASDILVPDHVTTTPS
                     IPITAYRDGFGNWCSRIVAPKGQIRIASTAVVKDTGEPDVVAPYAQQHSIQELPEDAL
                     VFLLGSRYCDTEKFS"
BASE COUNT          509 a          794 c          808 g          563 t
ORIGIN      
        1 tttgctcgag cctccgaatt tcgcgggttt cggcgggcaa atgagctgct ttcagcagtt
       61 ccgtgtattc ggccgctttt gcacgacatt gctttgctgt gaccatgaga cgagcccttt
      121 ctcgccagga gggagctcaa ccgtttctct cggtcaccgg ccctttcgtt ctgattatag
      181 gccggtgatg atcggactat ggggacgagg agctcagata gcaagggcgg gcgcctgcat
      241 tgaacccact aattctcgtt caaggcgaac aaacgcttgg gatgcaacaa ttgtgctcgt
      301 gatcggcgtc cagcgttccc ttagcgccct cggtttcact tggagacctg caggctggtg
      361 gtccgagtcc gacgcttgat acccaagcgt cgggcgggac caccggtgag ccgctgataa
      421 gcgtgcggaa gcaatcgcgc aaacaacgcc gcccacatcc gctgacccgc ctcgtccgtg
      481 atgaggcctt ggcgaatttc gagtgccaca tggtgcaagt cccgctgctc gccatggacg
      541 gggatcgtat agtccgacgc gtcggtgacg ctgtagggtt cgttgtcgcc gacgacgaga
      601 ccttcctcac gatgcaggag ttccatcaag atccgggcga aacgccggtc acggttgtag
      661 agcacgccga catgccaggg ccgcgccacg gtgttgaaaa cgggtgtgaa gctgtgcacg
      721 gaaatcaggg ctgtcggtag ggcggcttct cgtcgccgat cgagctcgat cgcgatgcga
      781 tcgtgatagg gctggaatat ctcccgatcg cgcgcgagct tgtgatcttc gctcaggccg
      841 atatttcccg gcacacacgt caactcgctc aggtcgagga ttgacgtctc cgaccccggc
      901 gttcggttgc agtcgatgac cagccgggaa tagttctgcc gaacgacgac ggcatcgagt
      961 gttcttccga tcaggcagca gaccgcgccg gcgccgatgt cccaggcaat gtgactctcg
     1021 ctttccgcat tggaaaggcc gagctgtcca agccgccgcg gaaaatgctt ccccgcgtga
     1081 tcggcgacga tgaggagcgg cgatgtgccg ttttcgttgt ctaccgtgaa aggcggcggt
     1141 tcgtcggtcg ccaaaagact gtcctgcgtt tcattcatct gcggtcactt ttgttgagaa
     1201 aaggatcgtc attggcgttg ccgtctttgc ttttggcgcg aaaaatgcat gctgttcaaa
     1261 gggcgaatca tgggggcggc catgacgatt ctcaccgtca ggcacatcac gacctatcag
     1321 tacaagcagc cggtctcctt cggcgagcac cggatgatgt tgcgcccacg ggaaggccat
     1381 gaccagagag tgctcgaatc aaagctcgag attactccga aaccgaccaa tctgcggtgg
     1441 atccgggacg tcttcggcaa ccatgttgcc attgcgcgct tcgccggccg ggccgacgtg
     1501 cttcgcttcg aaagtaccag ttgtctcgat cattccccgg ccgacattct cgacatggat
     1561 atcgaggatt ttgcgcgtac ctatccgttc atctacgggg cggaggacct gccggatctc
     1621 gtccgcttcg tcgaacggca ctcctcggac ccggatcatc aactcgatca gtgggtccgc
     1681 gcgctggtgc gcaagggtgg atcgatcaac acgcgtgcgc ttcttgtgaa tctgacccgg
     1741 accatcaagc agacctttac ccatgtggcc cggcatgaga aggggattca ggacccgctc
     1801 ctcaccctcc agatcggaag cgggagctgt cgcgacctcg ccatgctcat gatcgaggcg
     1861 gtgcgctcac ttgggatggc cgcacgtttt gtctcgggat atttgcatgt ccggacagac
     1921 cacacggagg gctgtgcccg cggcggcaac actcatgcct gggtccaggt ctatctgccc
     1981 ggccccggtt gggtcgattt cgacccggcg agtgggatcg tcggcaacca cgacctcgtg
     2041 cgtgtcgcgg tggtgcgcga tccccgccag gcgataccac tgcacggcac ttggaccggt
     2101 ttcccctcgg acaagttggg aatgaccgtc gaggtcaacg tgaccgccgc ggaagcgcaa
     2161 ctggccagcc gctccgcacg caccagttca gggatggcac tctaaggccg ccacgcgccg
     2221 tgcgccgatg gtgctgagca gatgcggaac agggtggaac caaatggtct ttcgctgctt
     2281 agctctatgg gcaagggaga tccccatgcg agcgttggag agatggaaat cagggtaggc
     2341 tacgagttga tctacgactg tcctcagccg acgccgatga tgctggtgct gaatattcac
     2401 tattcgcgtg catccgacat tctcgttccg gatcatgtaa cgaccacccc ctctattccc
     2461 ataaccgcct atcgcgatgg cttcggcaac tggtgcagcc ggatcgtcgc ccccaaaggc
     2521 cagatcagga tcgcgagcac cgcggtcgtc aaggacaccg gcgagccgga cgtcgttgcg
     2581 ccttatgcgc agcagcattc gatccaagaa ttgcccgaag acgcactggt ttttctttta
     2641 gggagccgct actgcgatac ggaaaagttt tccg
//
LOCUS       DBNGSB010000157         8684 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_1796768, whole genome shotgun sequence.
ACCESSION   DBNGSB010000157 DBNGSB010000000
VERSION     DBNGSB010000157.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 8684)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8684)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8684
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_1796768"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="AC159B_04515"
     CDS             complement(<1..719)
                     /locus_tag="AC159B_04515"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3377042.1"
                     /translation="MTTVALSLQRIQARTGWLARFIRRNPTGTAAGIAIFAMAVIAVL
                     APWVTPYDPLANDFTSMFSPPGPKHWLGADAFGRDVLSRLIYGARTALLVGFAASLSG
                     ATLGAAIGVVSAYFGGGTDILIQRLSDILLSFPIIILALAIVAILGSGTFNLTVAIAL
                     SFVPRIQLVVRASALGVRRTAYVEAARAIGAGHWRIIFRHMVPNVVAPYLIMITAFVA
                     QAILAEASLSFLGLGVTEPTAS"
     gene            complement(723..1676)
                     /locus_tag="AC159B_04520"
     CDS             complement(723..1676)
                     /locus_tag="AC159B_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3377043.1"
                     /translation="MTRFVVTRVFLIIPTLLAVATLIFVLVRVVPGDIVELRLVGGGS
                     FVTKGVIEAERARLGLNKPIWQQFVSWMAGLVQFDLGTSMWTGAPISQEIAIRLELSV
                     QLAVMSTLIAVALAVPLGTLAAARRGTVIDYAIQVFSVAGLATPSFWLGLLLILFLLT
                     TFHWLPPLTFTPLWQDPRANLIQLIWPALAVGYRYSAVTTRMTRSAVLEVLGEDYIRT
                     ARSKGLASSNVLRRHALRNAMLPVVTVVGLEFAFLIGGLVVTEQVFNLNGIGKLFVDA
                     ISHRDYTLIQGLMLLVATAYIFVNFMIDMLFAWLDPRISYG"
     gene            complement(1781..3370)
                     /locus_tag="AC159B_04525"
     CDS             complement(1781..3370)
                     /locus_tag="AC159B_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013912826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3377044.1"
                     /translation="MRKAIVTIVALVLVCLVAGTVSTTAQQPRRGGTLNFVVSAEPPS
                     LDAHRETTFALIHPVRPNYNLLVKFDPLNYPRVVPDLAESWSVSSDSLTYTFKIRRGV
                     RFHDGSTLTSRDVKASFDKIIFPQGDVVSARQAAYEMVAKIEAPDANTVVFTLKWRSG
                     SFVQNLASPYNWVYKADLLARDPHWYERNVMGTGPFKFGEYVRGSHWAGVRNESYWVS
                     GRPYLDGYRAIFTRGEAAKVNAVKSGQALVEFRGFSPAQRDETMQALGTQVQVQESGW
                     LCNNIVAFNTKRKPFDDARFRRALSLAVDRWGGSQALSRIAFVGPVGGLMRPGGPFRT
                     PDDELVKLAGYGRDIAANRAAARKLLAEAGIPEGFEFSLLNRNVPMPYEFVAIFLIDQ
                     WRQIGLKVNHVAKETSPYLADERAGNYDAAVDFACDFFDDPDQHLVKFLSADKSPLNY
                     GGYFDRTLDRLYNEQSRERDPTRRLRLVRQFEKRLLDERVYIMYVLWWQRIIPHSTKL
                     RNYKVGPSHYLEDLQDVWISE"
     gene            3513..4502
                     /locus_tag="AC159B_04530"
     CDS             3513..4502
                     /locus_tag="AC159B_04530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HIR3377045.1"
                     /translation="MRKTLALCAVALLLLTVPAALDAQQRAGWPRSVTIGSASIGGVY
                     YVVAGGYARLIGEKMGLAATNRVTGGPVQNVQLVGTKEIELGLVTTGPAFEAYNGIGE
                     FAGKKIDTLRVAFPMYTSYAHWMVSADSPIRSVTDLNGRVVSLGPRGGSAEFVGDRVL
                     QQFNISTRRVVYLGFADGASAMRDGVVDANFGVIGVPVPAWVEASVTRPARFFGFTPA
                     QVETLTRQYPYLARAAIRAGVYRGQDAPIPTLQMWNAAIVHKDMPEDFVYELVKAVFA
                     NIDFLETVHPTAGETIPVNIDYIRLPLHPGALRFFQERGVRLRPEHFPPEARR"
     gene            4609..6555
                     /locus_tag="AC159B_04535"
     CDS             4609..6555
                     /locus_tag="AC159B_04535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HIR3377046.1"
                     /translation="MSQEPSGLPVDTSQLGDASELTRSRPLGGALRLLVRAVAAAMAI
                     YHVLVLGRFFGIVTDPQKVYAMHLTFVAVLAFLWVPGRRRQLRPTVIDWLLIAATIVV
                     VAYVFLVFEDLTGRAGFSPTREDVLVGSLLVIVTLEASRRAIGWSLPLIGMAFAIYPF
                     IGPWMPGLFEHRGFSVNTVISFLFSDNAIYGIPIQVSARDVYLFILFGAFLEASNIGK
                     FIVNLGLSVAGGRRGGPAKVSILTSALFGTASGSSAANVMVDGVINIPLMKATGFKAT
                     VAGGIEAMNSTGGQIVPPVMGAAAFLMADILGVPYARIAVAAIGPALLYYVAAYWMID
                     LYASSHGLRGLPRQDLPRFGAIMVQHGYMLAPLVVLLYLIIVTGASPFRAALYALFLT
                     VAVSLVRPDTRLNLTKIINAMEEGAKRTIEIGVSCAAAGIIVGVLALTGLGGKFSEVL
                     INLTGGSLLLALIGTMVAAIVLGTGLPTTAAYAIGASVLAPALIKLGALPIAAHMFLF
                     YFSVISAVTPPIAFASFAAASIAKAPMWDTSVESMRFGIAGYIVPFMFVYGTSLLVGT
                     HPWPQTLLALASGTLGTLALAAATVGYLTRKATALERLALLASSLLLIRPGLGTDLIG
                     LGLLSGVFVLHRFLKVSVAVPGQT"
     gene            complement(6569..7813)
                     /locus_tag="AC159B_04540"
     CDS             complement(6569..7813)
                     /locus_tag="AC159B_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012061957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbE family protein"
                     /protein_id="HIR3377047.1"
                     /translation="MDIEAANKEAARRILESTSVLVGIARADEVIPKMRKNLVLHAGP
                     PIAWNRMSGPLRGAVIGGLLLERLATDERQAVAMVERGEIEFAPCHHFQAVGPMAGVT
                     TASMPVYIVENKAFGNRAFSNLNEGYGKVLRYGAYSEDVLARLRWLADVFAPMTGQAL
                     ARSGGIDMKNMIAQQIQMGDEGHNRNKAGSAMFARLLAPHLARGGGRSEDIAAILDHL
                     AGNDLAILNPVMAACKAMVDAAHGVEGSTVVTCMARNGTDFGIRVSGLRDRWFTAPAD
                     VPVGLFFPGYSQEDANPDIGDSTITETAGIGAFAMAAAPAIVKFVGGTPKMAFESTME
                     MYEITVTENPSFGIPQLDFRGTPTGIDIRRVVKTGILPRVNTGIAHRKPGIGQVGAGL
                     VRPPMACFEQALEAMANLVADK"
     gene            complement(7858..>8684)
                     /locus_tag="AC159B_04545"
     CDS             complement(7858..>8684)
                     /locus_tag="AC159B_04545"
                     /inference="COORDINATES: protein motif:HMM:NF012758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein FdrA"
                     /protein_id="HIR3377048.1"
                     /translation="SLTAQGVRVIDVDWQPPAGGKQKFIRGLYSGGTLCYEALTLLER
                     YVGPVHSNTPIDKAFALETAHRSRAHTVIDMGSDEFTVGRLHPMIDPSLRLERILKEA
                     EDPETAVILLDVVLGYGAHPDPANELALIIRKAKQTARAQGRWLSVVLSVCGTEEDPQ
                     GYNDQVSALVEVGVVVMPSNAQAVRMAGLIAEGAGNKGKVREAVLTVRPSDPVSLPDV
                     TLIRPLLAEAPRVVNIGLGLFAESLTAQGVRVIDVDWQPPAGGKQKFIEMLDKLNA"
BASE COUNT         1549 a         2789 c         2694 g         1652 t
ORIGIN      
        1 gacgccgtcg gctcggttac gcccaaaccg aggaaggaga gcgacgcctc ggccagtatc
       61 gcctgagcca cgaaagcggt gatcatgatg aggtagggcg cgaccacgtt cggcaccatg
      121 tgccggaaga tgatgcgcca gtggcccgcg ccgatcgccc gcgctgcttc cacgtaggcc
      181 gtccgccgca ccccaagcgc gctcgcccgc acaacgagtt gaattctcgg gacgaaagaa
      241 agcgcgatcg ccacggtcag gttgaacgtg ccgctcccca ggattgccac gatcgccagt
      301 gccaggatga tgatcggaaa cgagagtaag atgtccgaca ggcgctggat cagaatgtcc
      361 gtgccgccgc cgaaatacgc gctcacgacg ccgatcgctg cgccgagcgt cgcgccggag
      421 agggaggccg caaatccgac gagcagcgcc gtgcgcgcgc cgtagatcag gcggctgagc
      481 acgtcacgtc cgaaggcgtc tgcccccagc cagtgcttcg ggccgggcgg agagaacatc
      541 gacgtgaagt cgttggccag agggtcataa ggcgtaaccc agggtgccag tacggcaatc
      601 accgccatgg cgaagatcgc gatgcccgcc gcggttccgg tcggattgcg tcggataaaa
      661 cgggcgagcc agcctgtcct cgcctgaatt ctctgcaggg aaagcgcgac tgtggtcatt
      721 gctcacccat aggagatccg gggatcgagc cacgcgaaca gcatatcgat catgaagttc
      781 acgaagatgt atgccgtggc gacgagcagc atcagcccct ggatcaacgt atagtcgcga
      841 tggctgatcg cgtcgacaaa caacttcccg atgccgttga gattgaatac ctgctcggtg
      901 accaccagtc caccgatgag gaacgcgaac tccaggccga cgacggtcac caccggcagc
      961 atcgcgtttc gcaacgcatg gcggcgcagc acattgctgg atgccagccc tttcgaccgc
     1021 gccgttcgga tgtagtcttc acccagcact tccagcaccg ccgaccgcgt catgcgcgtg
     1081 gtcaccgcgg agtaccgata gccgacggca agggcgggcc agatcagctg gatgagattg
     1141 gcccggggat cctgccaaag cggggtgaac gtcagcggag gcagccagtg gaacgtcgtg
     1201 agcagaaaga gaatcaggag gagacccagc cagaacgatg gcgtggcgag gcccgccacg
     1261 ctgaacactt ggattgcgta atcgatcacg gtgccgcgac gtgcggcggc cagcgtgccc
     1321 agcggaaccg ccagggccac cgcaatcaac gttgacatca cggccagctg gacgctcagt
     1381 tcgaggcgga tcgcaatctc ttgcgaaatc ggcgcccccg tccacatcga tgtcccgagg
     1441 tcaaactgga cgagccccgc catccagctc acaaactgct gccagatcgg tttgttcagc
     1501 ccaagtcgag cccgttcggc ctcaatgacc cctttggtga caaatgagcc gccgccgacc
     1561 aggcgcagtt cgacgatatc gcctggaacc acgcgcacca ggacaaagat gagagtcgcc
     1621 acggcaagga gcgtgggaat gatcagaaac acgcgggtca cgacgaagcg cgtcacagga
     1681 aatcaacgcg acccccgtcg gctttcgacg gagggtcgcg tcgcttcact ctccagacca
     1741 tcgtgacccg gctacccggt tccccggatt gccgacgctc ctactccgag atccagacgt
     1801 cctgcagatc ctccaggtag tgtgagggcc caactttgta gttcctcagt ttcgtcgagt
     1861 gagggatgat ccgttgccac cacaggacat acatgatgta gactctctcg tccagcagcc
     1921 gcttctcgaa ctgccggacc aggcgcaggc gtcgggtggg atcgcgctcg cggctctgtt
     1981 cgttatagag ccgatcgagg gtgcgatcga agtagccccc gtaattgagc gggctcttgt
     2041 ccgcagagag gaacttcacc aagtgctggt ccggatcgtc gaagaagtca caggcgaaat
     2101 cgaccgcggc atcatagttg ccggcgcgct cgtccgccag ataggggctc gtttccttgg
     2161 ccacatgatt caccttcagc ccgatctgtc gccactggtc gatcaagaaa atggccacga
     2221 actcgtacgg catcgggaca ttgcggttga gcaaggaaaa ctcgaacccc tcggggatgc
     2281 cagcctcggc tagtaacttc cgcgccgcgg cccgattcgc tgcaatatcc ctcccgtatc
     2341 cagcgagctt gacaagttca tcgtccggtg tgcggaacgg cccgcccggt cgcatcaggc
     2401 cgcccacggg tcccacaaac gcgatccgcg acagcgcctg ggaaccgccc cagcggtcca
     2461 cggcgaggga gagggcgcgg cggaacctgg catcatcgaa tggcttccgt ttggtgttga
     2521 aggcgacgat gttgttacac agccaaccgc tttcctggac ctgaacctgg gtcccgagcg
     2581 cctgcatcgt ttcatcgcgc tgagccggcg agaagccgcg gaattctacc agcgcctgac
     2641 cactcttgac ggcgttgact ttggcggcct cgccgcgggt gaagatggct cgatatccgt
     2701 ccagatacgg ccggccgctg acccagtagc tttcgttgcg aacgccggcc cagtgtgagc
     2761 cccggacata ctcgccgaac ttgaacggcc ccgtccccat gacattgcgt tcgtaccagt
     2821 ggggatcgcg cgccaacagg tcggccttgt acacccagtt gtacggcgag gcgaggttct
     2881 gcacgaacga tcccgagcgc cacttcaggg tgaagaccac ggtgttggcg tcaggcgcct
     2941 cgatcttggc gaccatctcg tacgccgctt ggcgggcgct cacgacgtcg ccctgcggga
     3001 agatgatctt gtcgaagctg gccttcacgt cccgggacgt cagcgtgctc ccgtcatgga
     3061 accgaacgcc acgcctgatc ttgaaggtgt aggtgagact atcagaggac acggaccagc
     3121 tctccgcgag gtcgggcacc accctcgggt agttcaatgg gtcgaacttc accagcaggt
     3181 tgtagttggg gcgcacggga tggatgagcg cgaaggtcgt ctccctgtgc gcgtccagcg
     3241 acggcggctc cgctgagacg acgaagttta acgtcccacc ccggcgtggc tgctgcgcgg
     3301 tggtcgacac cgtgccagcg acgaggcata caagcaccaa cgccacaatg gtgacaattg
     3361 ctttccgcat atatctccaa cctcccgaca agactgttgg atttgtgtgt ccactacgac
     3421 acgtccaacg cccttacctg tagtgccgag gagtcggctt tcaatccagc gaacacgtca
     3481 ggcatcagat gtgaaataag aggaggaacg tcatgcgcaa gacacttgcc ttgtgtgctg
     3541 tcgccctgct gctgctcacc gtcccggcgg ccctcgatgc tcagcagcgt gcgggctggc
     3601 cgcggtcggt caccatcggc tccgcgtcaa tcggcggcgt gtactacgtc gtcgccggcg
     3661 ggtacgcgcg gctcatcggc gagaagatgg gcctcgccgc taccaaccgg gtcaccggcg
     3721 gcccggtgca aaacgtgcag ttggtcggca cgaaagaaat cgagcttgga ctggtcacaa
     3781 cgggccccgc gttcgaggca tataacggga tcggcgaatt cgcggggaag aagatcgaca
     3841 cgttacgcgt cgccttcccc atgtacacgt cttacgccca ctggatggtc agcgcagact
     3901 cgcccatccg ttccgtcacg gacttgaacg gccgcgtcgt cagccttgga ccgcgcggcg
     3961 gttctgcgga attcgttggt gaccgtgtgc ttcagcagtt caacatcagc acgcggcgcg
     4021 tcgtctatct cggcttcgct gacggcgcgt cggcgatgcg ggacggcgtc gtggacgcca
     4081 acttcggcgt catcggcgtt ccggttccgg catgggtgga agcgtcggta acacgcccgg
     4141 cgcgcttctt cgggtttacg ccggcgcagg ttgagacgct aacgcggcag tacccgtacc
     4201 tcgcgcgggc cgcgatccgc gcgggcgtgt accgcgggca ggacgcgccg atcccgacgc
     4261 tgcagatgtg gaacgcggcg atcgtgcaca aggacatgcc cgaagacttc gtctatgaac
     4321 tggtgaaggc cgtctttgcg aacatcgact tcctggagac ggtccacccg acggcgggag
     4381 agacgatccc cgtgaacatc gattacatcc ggcttccgct gcatcccggc gccctgcgct
     4441 tcttccagga gcgaggcgtc aggctgcgtc ccgaacactt ccctccggag gcgagacgct
     4501 aaggtcaggt ggcgcccacg agtgttctgt ttgagttcat cggcggcgca cagaggccga
     4561 gctacggcga ggccggggga attttgcttg gtgccgggtt aagtagcggt gagccaggag
     4621 ccctccggtc tcccggtcga tacctcgcaa cttggtgatg cctccgaact aacccgctcg
     4681 cggccactcg gcggcgcgct ccgcttactg gtgcgcgccg tcgccgcggc aatggcgatt
     4741 tatcacgtcc tcgtgctggg ccggtttttc ggcattgtga ccgacccgca gaaagtctac
     4801 gcgatgcacc tcacgtttgt cgcggtgctg gcgtttttgt gggttccggg ccggcgcagg
     4861 caactgcgtc cgaccgtgat cgactggctg ctgatcgccg ccactattgt cgtcgtcgca
     4921 tacgtcttcc tcgtcttcga ggatctcaca ggccgtgccg gcttctctcc aacgagggaa
     4981 gatgtgctcg tcggttcact tttggtcatt gtcactctcg aggcttcgcg gcgcgccatc
     5041 ggctggtcgt tgcccctgat aggcatggcg ttcgcgattt atccattcat tggaccctgg
     5101 atgcccggat tgttcgaaca tcgtggcttt tcggttaaca ccgtcatctc gttcctgttc
     5161 agcgacaacg ccatctacgg catccccatt caggtatcgg cgcgcgacgt ctaccttttc
     5221 atcctcttcg gcgcgttcct cgaggccagc aacatcggca agttcatcgt caatctggga
     5281 ctcagtgtcg ccgggggccg ccggggggga ccggctaagg tgtctattct gacgagcgcc
     5341 ctcttcggca ccgcgagcgg gtcttctgcc gccaacgtga tggtggacgg cgtcatcaac
     5401 atcccgctga tgaaggcgac agggttcaag gcgaccgtgg ctggagggat cgaggcgatg
     5461 aacagcaccg gggggcagat tgtgcctcct gtcatgggcg ccgccgcgtt tctcatggcg
     5521 gacatcctcg gcgtcccgta cgccaggatc gcggtggcag cgatcgggcc cgcgctgctg
     5581 tattacgtcg ccgcctactg gatgatcgac ctgtacgcat cttcgcatgg gttgcgcggt
     5641 ctgcctcgcc aggatcttcc ccgtttcggc gcgatcatgg tgcaacacgg gtacatgctg
     5701 gcgccgcttg tcgtactgct gtacctgatc atcgtgacgg gagcgtcgcc gttccgagcc
     5761 gcgctctatg cgttgttctt gacggtcgcc gtgtcgctcg tgcgtcccga tacccggctg
     5821 aacttgacga agatcatcaa cgcgatggag gaaggcgcaa aacgcacgat cgagatcggc
     5881 gtctcctgcg ccgccgccgg gatcatcgtg ggcgtcctgg cgctgaccgg actgggggga
     5941 aagttctccg aggtcttgat caacctgacc ggtgggagcc tgctgctcgc gctcatcggc
     6001 accatggtcg cggccatcgt cctcggtacg gggttaccga cgaccgccgc gtatgccatc
     6061 ggcgcgagcg tcctcgcccc cgcgttgatc aaactcggcg cactgcccat cgccgcgcac
     6121 atgtttctgt tttatttctc ggtgatctcc gcggtgacgc cccccatcgc atttgcttca
     6181 ttcgccgcgg ccagcatcgc gaaagcgccg atgtgggata cgtcggtcga gtcgatgcgc
     6241 tttggcatcg caggctacat cgtgcccttc atgttcgttt acggcacatc attgctggtg
     6301 ggcacgcacc cctggcccca gaccttgctc gcgttggcct ccgggacact aggcacgctg
     6361 gcgctcgcgg cggcgacggt cggctatttg acgcgcaagg cgactgcact agagcggctg
     6421 gcgctgcttg cctcgtcttt gctgctgatc agacccggct tggggaccga cctgatcggt
     6481 ctcgggctgc tgtccggcgt tttcgtcctc catcgtttcc tgaaggtgtc cgtggcggtt
     6541 cctggccaga cgtaaaaggg gttgtgagct atttgtcggc gaccaggttg gccatcgctt
     6601 cgagcgcctg ctcaaagcat gccatcgggg ggcggacgag cccggcgccg acctgaccga
     6661 ttcctggctt gcggtgcgcg atgccggtgt tgacgcgagg gaggatccct gttttgacga
     6721 ccctgcggat gtcgatgccg gtcggtgtgc cgcggaaatc gagctggggg atgccaaagc
     6781 ttgggttttc ggtcacggtg atctcataca tttccatcgt ggactcgaac gccatcttcg
     6841 gggtgccgcc gacgaacttg acgattgccg gcgcggccgc catcgcgaac gcgccgattc
     6901 cggccgtctc cgtgatcgtg ctgtcaccga tgtcggggtt ggcgtcttcc tgtgagtagc
     6961 ctggaaagaa cagaccgacc ggcacatcag ctggcgcggt gaaccagcgg tcgcggagcc
     7021 cgctgacgcg gatgccaaag tcggtgccgt tgcgggccat gcacgtcaca accgtgctgc
     7081 cctccactcc gtgcgcggcg tccaccatcg ctttacaggc ggccatcacc gggttcagga
     7141 tggcgaggtc attcccggcg agatggtcga ggatcgcggc gatgtcctcg ctccttccgc
     7201 ctccgcgcgc caggtgaggc gcgaggaggc gcgcaaacat cgcggaccca gccttgttgc
     7261 gattgtgtcc ctcgtcgccc atctggatct gctgggcaat catgttcttc atgtcgatgc
     7321 cgccggagcg cgccagcgct tggccggtca tgggggcgaa cacgtccgcg agccagcgca
     7381 ggcgggcgag cacgtcttcg ctgtacgcac cgtaccgcag gaccttgccg tacccttcgt
     7441 tgagatttga aaacgcgcga ttcccaaagg ctttgttctc aacgatgtag accggcatcg
     7501 acgccgtcgt aaccccggcc atcggcccga ccgcctggaa gtgatggcac ggcgcgaact
     7561 caatctcgcc gcgctcgacc atcgccacgg cctggcgttc atccgtcgcg agcctttcaa
     7621 gcaagagacc gccgatcacc gcgccgcgca gcgggccgga catccgattc cacgcgatcg
     7681 ggggacccgc gtgcaggacg agattcttgc gcatcttcgg aatcacttcg tcggcgcgcg
     7741 cgatgcccac gagcaccgag gtgctttcaa ggatgcggcg agcggcctct ttattggctg
     7801 cttcgatgtc catatcgcgt ccagccgacg gtgttcggaa cccgaacacc gtcgctttca
     7861 tgcattcagc ttgtcgagca tctcgatgaa cttttgcttg ccgcctgccg gcggctgcca
     7921 gtccacatca atgacgcgga ccccttgagc ggtcaggctc tcggcaaaca accccaaccc
     7981 aatattcacg accctcgggg cttcggccag cagcgggcgg atcaatgtga cgtccggcaa
     8041 cgaaacgggg tccgagggcc gcaccgtcag taccgcctca cgcaccttcc ccttgtttcc
     8101 tgccccctcg gcgatcaagc ccgccatccg cacggcctgc gcgttgctcg gcatcaccac
     8161 caccccgacc tccacgagcg cgctgacctg gtcgttgtaa ccctgcggat cctcctccgt
     8221 tccacacacg gacaagacaa ctgaaagcca gcgtccctgc gccctggcag tctgcttggc
     8281 cttgcggatg atcagcgcaa gctcgttcgc cggatcggga tgcgcgccgt atcccagcac
     8341 gacatccagc aaaatcacag cggtttcggg gtcctcggct tctttgagaa tgcgctccag
     8401 acggagactg ggatcgatca tcgggtgcaa gcgccccacg gtgaattcgt cgctccccat
     8461 gtcgataacc gtatgcgcgc ggctgcgatg agcagtctcc aacgcaaatg ccttatcgat
     8521 tggggtgttg gaatggacgg gaccgacgta gcgctcgagc aaggtcaatg cctcataaca
     8581 cagcgtcccg ccgctgtaga gccctcggat gaacttttgc ttgccgcctg ccggcggctg
     8641 ccagtccaca tcaatgacgc ggaccccttg agcggtcagg ctct
//
LOCUS       DBNGSB010000158         4058 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2104716, whole genome shotgun sequence.
ACCESSION   DBNGSB010000158 DBNGSB010000000
VERSION     DBNGSB010000158.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4058)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2104716"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1523)
                     /gene="hutU"
                     /locus_tag="AC159B_04550"
     CDS             complement(<1..1523)
                     /gene="hutU"
                     /locus_tag="AC159B_04550"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HIR3377049.1"
                     /translation="MRMLMNNLDPEVAERPDELIVYGGTGKAARNWACFDAIVTSLRS
                     LADDETLLVQSGKPVGVFRTHPWAPRVLISNSMLVPAWATWEKFWELEALGLMMYGQM
                     TAGSWIYIGTQGILQGTYETFAAVARSHFGGSLSGRTVLSAGLGGMGGAQPMGVTMND
                     GAALIVEVDPARIHRRISAGWVDRATDHLDEALRWIDEAKRARRASAVALLGNAAEIF
                     PELVRRRIVFDIVTDQTSAHDPLGGYIPEGVTAEEAPQLRQRDPDDYVKRSRASMMRH
                     CDAMVKMQLSGSVVFDYGNKLRGQAREAGFEGAFSYPGFVQAYIRPQFCEGRGPFRWA
                     ALSGEPRDIHRTDQVVLELFPRHDSLARWIRLAEERVPFQGLPARICWLGYGERAQAG
                     LAFNELVRRGDVAAPIVIGRDHLDAGSVASPYRETEGMRDGSDAVADWPILNALVNAS
                     AGATWVSVHHGGGVGIGYSIHAGQVVIADGTPLGDEKLERVLTSDPGTGIMRHADAG"
     gene            complement(1628..3151)
                     /gene="hutH"
                     /locus_tag="AC159B_04555"
     CDS             complement(1628..3151)
                     /gene="hutH"
                     /locus_tag="AC159B_04555"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HIR3377050.1"
                     /translation="MTVEIGEHLTLEDVVTVARERAAVALSPHARTGVERSRAVVERL
                     AGEGRAIYGISTGVGELAGVHISPEQTAQLQINIVRSHSAGVGEAFPEEVVRAMMLLR
                     AHALALGYSGIRLQTLDLLIQMLNQGIHPVVPSQGSVGASGDLAPLAHLALAMIGENA
                     ATVNGQRVSSREALAKVRLSPVVLAAKEGMALINGTQAMTALGALAVIDAQRLADTAD
                     VAAAMTFEALRGLPDAFDALLHQVRPHPGQRASAENLRRLITGSEILQQAPPRSKVQD
                     AYALRCIPQVHGASRDAIAYARRVIEIEINSATDNPLIFPDDDRVISGGNFHGQPVGL
                     ALDFLAIAVSGLANIAERRIERLVNPHLSGLPAFLTSDGGLHSGLMLAQYTAAALVSE
                     NKVLSHPASVDSIPTSANQEDHVSMGTIAARKAARVLAHAQQVIGIELICAAQALDFH
                     HPLRPGKGTGRAYDAVRRSILPLKQDRVLADDLAKGFELVRDGSVLAAAEQLVGRLS"
     gene            complement(3148..3831)
                     /locus_tag="AC159B_04560"
     CDS             complement(3148..3831)
                     /locus_tag="AC159B_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0033293 - monocarboxylic acid binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl attachment domain-containing
                     protein"
                     /protein_id="HIR3377051.1"
                     /translation="MAKPGRLVRKTGVSLGPILIYVAAVVVVSGGLYAWDSAYRRREE
                     EARKPPPTDVVAKNLVENIIGRGTVKDVKVNESAGTVEVTFESATYPPAARATVDGEI
                     VGQGLAKVGTRVRKGAAVASVKASDGKVVVAARADYTGAVAQVLVKPGDKIEKDRAVL
                     TIVPDDKTEAKQNLETEGLLASQAILSQLNTMKTVTSKIIYSNVVLATVVGSRGEKAV
                     RSTFHESLK"
BASE COUNT          740 a         1361 c         1209 g          748 t
ORIGIN      
        1 taccctgcat ccgcgtggcg catgatgccg gtcccgggat ccgaggtaag gacgcgctcg
       61 agtttctcat cgccgagagg agtgccgtcg gcgatcacga cttgtccggc gtgaatggaa
      121 tacccgatgc ccacaccgcc accatggtga acggacaccc aggtagcacc ggcgctggca
      181 ttcacgagcg cgttcagaat gggccagtcc gcgactgcgt cggatccgtc cctcatccct
      241 tcggtctccc ggtacgggga tgcgacggag cccgcgtcga gatgatctcg gccgatgaca
      301 atcggggccg cgacgtctcc ccggcgcacc aactcgttga acgccaggcc ggcctgcgct
      361 cgctcgccat agcccaacca gcagatgcgc gcgggcagcc cttgaaacgg cacgcgctct
      421 tcggccaggc gaatccatcg tgccagcgag tcatgcctgg ggaacaattc cagaacgacc
      481 tgatccgtac ggtgaatgtc tcgcggctcg cccgagagcg cagcccagcg aaacggtccc
      541 cggccttcgc agaattgtgg ccggatatat gcctggacga acccagggta gctgaaggcg
      601 ccctcgaaac cggcttcacg cgcttgaccc cgcagtttgt tgccgtagtc gaagactacg
      661 cttcccgaca gctgcatttt gaccatcgca tcgcagtgac gcatcatcga tgctcgagag
      721 cgcttgacgt aatcgtcggg gtcccgctgc cgcaactgcg gcgcctcctc cgcggtgacg
      781 ccctcgggaa tgtagccgcc caatggatcg tgcgcggacg tttgatcggt gacgatatcg
      841 aacacaattc tccgccgcac cagctcggga aaaatctccg ccgcgtttcc aagtaaggcg
      901 acggcggacg ctcttctcgc gcgcttggct tcatcgatcc agcgcagggc ctcgtcgagg
      961 tgatcggtgg cgcggtccac ccagcccgcg ctgatgcgcc ggtgaattcg tgccgggtcg
     1021 acttccacga tgagcgccgc gccgtcgttc atggtaacgc ccatcggttg ggcgccaccc
     1081 atcccaccga gtccggccga caggaccgtg cggccgctga ggctgccacc gaagtgggac
     1141 cttgcgaccg cggcaaatgt ctcatacgtt ccctggagaa tgccctgggt cccgatgtag
     1201 atccatgagc ccgccgtcat ctgaccgtac atcatgaggc cgagcgcttc gagttcccag
     1261 aacttctccc acgtcgccca tgccgggacc agcatcgagt tgctgatgag cacgcgcggc
     1321 gcccacggat gcgttcgaaa gaccccgacg ggtttgcctg actgcacgag cagcgtttca
     1381 tcgtcggcga gcgaccggag gctggtcaca atcgcgtcaa aacacgccca attccgagcc
     1441 gccttgccgg tgccgccgta gacgatgagc tcgtcgggcc gctccgccac ctctggatca
     1501 aggttgttca tcagcatccg catcgcggct tcctgcggcc atccccgaca ggagatcgcg
     1561 cttccccgag gagccctgac gcgccgcgcc tggcctgaac tcgtacgggg tgactgaccc
     1621 gggatgctca cgacaggcgc cccaccaact gctcggcggc ggccagcacc gatccgtcgc
     1681 ggacaagctc gaagcctttc gccagatcgt cggcgagcac acgatcctgc ttcaacggaa
     1741 ggatactgcg gcggaccgcg tcatacgcgc ggcccgtgcc cttgccgggc cgcagcggat
     1801 gatgaaagtc cagggcctgc gccgcgcaga tgagctcgat accgatgacc tgctgagcgt
     1861 gcgccaggac gcgggccgcc ttgcgagccg cgatcgtgcc catgctgaca tgatcttcct
     1921 gattggcaga ggtcgggatg ctgtccacac ttgccggatg actgaggact ttgttttcgg
     1981 agacgagcgc ggccgccgta tattgggcca gcatcaaccc cgaatgcagc ccaccgtcgc
     2041 tcgtcagaaa cgccggcaaa ccactaagat ggggattgac gagccgctcg atccggcgtt
     2101 cggcgatatt cgcgagcccg ctcaccgcga tggccaagaa gtcgagggcg agtccgacgg
     2161 gctgaccatg aaagttcccg cctgatatca cacgatcatc atccgggaag atcaatgggt
     2221 tgtccgtggc ggagttgatc tcgatctcga tgacacggcg ggcgtacgcg atggcgtcac
     2281 gtgacgcccc gtgcacctga gggatgcagc gcagcgcgta ggcatcctgc actttcgatc
     2341 tcggcggggc ttgttggagg atctcgctcc ccgtgatcag gcgccgcagg ttctccgcgc
     2401 tggcgcgctg ccccgggtgg ggacgcacct gatgcaagag cgcatcaaac gcgtcgggca
     2461 atccccgcag cgcctcgaac gtcatggccg ccgccacgtc cgccgtatca gccagacgct
     2521 gcgcgtcgat gacggcgagc gcaccgagcg cggtcatcgc ctgcgtgcca ttgatcagag
     2581 ccatgccctc tttggcggcg agcacgaccg gcgacagacg caccttcgcg agggcctccc
     2641 ggctcgacac tcgttgcccg ttcactgttg ccgcgttctc accgatcatt gccagcgcga
     2701 gatgcgcaag cggcgcaaga tctcctgacg cgcccacaga tccttgcgac ggaacaacgg
     2761 ggtgaatccc ttggttcagc atttgaatga ggaggtccag tgtctggaga cggatccccg
     2821 agtacccgag cgcgagcgca tgtgcgcgga gcagcatcat ggcccggacg acctcttcgg
     2881 ggaacgcctc gcccacgccg gcgctgtggc tccgaacaat attgatctga agctgcgccg
     2941 tctgctcggg agagatgtgt acgcccgcca gctcgcctac gccggtgctg atcccgtaaa
     3001 tggcccgccc ttccccggcg agccgttcca ccacggcgcg cgatcgctcc acaccggtcc
     3061 gagcatgagg agagagggca actgctgccc tctctcgtgc gaccgtgaca acatcttcca
     3121 gcgttaaatg ttcgccgatc tctacggtca cttcaaactc tcgtgaaagg tggagcgcac
     3181 cgccttctct ccgcgcgaac ccacgaccgt ggccagcaca acgttgctgt agatgatttt
     3241 cgacgtgact gtcttcatgg tattgagctg gctcaagatg gcttgagacg caagcaggcc
     3301 ctcggtctcg aggttctgct tcgcttcggt cttgtcgtcg ggaacgatcg tcaggaccgc
     3361 gcggtctttt tcgattttat ctcctggctt gacgaggact tgcgccaccg cgccggtgta
     3421 atcggccctc gcggcgacga cgacctttcc atcactcgcc ttcacgctgg cgacggctgc
     3481 ccctttcctg acgcgcgtac caacttttgc caggccctgc ccaacaatct ccccgtccac
     3541 cgtcgcacga gccgccggag gatacgtcgc ggattcaaac gtcacttcga ccgtcccggc
     3601 cgattcattc accttgacgt ctttgaccgt cccgcggccg atgatatttt cgacgagatt
     3661 cttcgcaacg acgtctgtcg gagggggctt ccgggcctct tcttcacgcc gccggtaagc
     3721 cgaatcccag gcgtacagcc cgccgctgac tactacgacg gcggcgacat aaatgagaat
     3781 cggaccgaga ctgacaccgg tcttgcgcac taatctgcct ggctttgcca cccactcacc
     3841 cccgaatcca gtaaccagtc ggcagtacgc agtaaccagt caaaaagggc agactatgac
     3901 ttcgcggtcc tactgcctac tgcctactgg cgacggccta cttctgccga ctaggagaca
     3961 acgatcgcat cagaggtagt aatcgttcct ctatagagga acgatgtttg cctgatcgct
     4021 gcctggaaat caaagggtgt gatcgccgag atggcgtc
//
LOCUS       DBNGSB010000159        13722 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_1032431, whole genome shotgun sequence.
ACCESSION   DBNGSB010000159 DBNGSB010000000
VERSION     DBNGSB010000159.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 13722)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13722)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13722
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_1032431"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..1202)
                     /locus_tag="AC159B_04565"
     CDS             complement(24..1202)
                     /locus_tag="AC159B_04565"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3377052.1"
                     /translation="MQRVLIVFAVITLLALPTAAQQQTVKVGFVVDITGPASSLGVPE
                     RNTVLMYQDDFATIQGPAGPVRIQYLVTDGESDPTKAVIAAKRLIEEDRVVAVVCCTT
                     SPASLAILQTVQAARTVNVSLAAAVTIIEPVEERRWIFKTPQTDRLMIDVLTDHMRQR
                     GIRRVAWLGFDDAFGQVGLGEFDKFAPQKGIEIVARESFARTATDVSTQITRAMGRNP
                     EAFLIWAIPPGANVSQRNIRDLGINLPVYQSHGVANKTFLDLGGPNVEGTLLPAGKLL
                     IAELLPEADKQKRALLGYLERYEGRYGRGTRNTFGGHALDAMLILQPAIERTLRQGIG
                     PDNIGGFRSVLRDQIERGTKELVGITGIFTFTAKDHLGLDRRAAVMIEVRNNNWAPAR
                     "
     gene            complement(1219..1962)
                     /locus_tag="AC159B_04570"
     CDS             complement(1219..1962)
                     /locus_tag="AC159B_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3377053.1"
                     /translation="MTLLLQAEHLAASYGPVQAVRDVSLTVAESEVVAIIGPNGAGKT
                     TLLSALAGLVPAAGVITFAGRHIDGIAPEDVVRLGMSLVPERRQLFGSMSVRDNLLLG
                     AYHRFGSETANAIEHDIREVFELFQVLKGRAGHPAMTLSGGMQQMLAIGRGLMARPRL
                     LLMDEPLLGLAPLVIKEIVRTLRELQSRGQSILIVEQNAKAALALADRAYVMEGGRVV
                     LAGRAAELGEDPKVQAAYLGGHVEPAQER"
     gene            complement(1959..3755)
                     /locus_tag="AC159B_04575"
     CDS             complement(1959..3755)
                     /locus_tag="AC159B_04575"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HIR3377054.1"
                     /translation="MTQRAWLLGLIIVLIITPSLVAGRVSLGIFIFIGLFSLIVIGLT
                     LLAGHAGQVSLGQAAFYGIGAYVSAITTLRLGLPPFLGVMLAVSGAAFIAYVIGAPIF
                     LLRGHFLVLGTLGFNIVVDVVIRNVQGLTGGPTGLTGIPPLSVGRLIIVGDRAYYYLI
                     WTLVLLALWLGRNLIHSRIGRALAAIRASEVTAATLGINPAFFKARMLALSAGFAGLA
                     GAIYVHYLGFVSPSPFAFNFSIDLLVMSVLGGIAFLPGAVLGTAVLTVLREVLRTIMP
                     RLLGTGASAEYEIVIFGLLLAVVVVLAPGGIWPWVAAVFRLNAKRPEFRQESVAAGLA
                     PSPSLSLQRGETLLKVDKLQKRFGGLLAVSGLSFEVKQGEIYAMIGPNGAGKTTAFNL
                     ISGVLRATSGDIAFKGQTITRLQPYAIAGLGVARTFQTPRVFGDLTVLNNVMIGMHRS
                     LGTGFLGSMLGLGRREDRRAAADAAGYLERVGLLDRAMDRSGALPFGAQRFLELARAL
                     AAQPALLLLDEPASGLSTSERGELVRLIRQIRDAGITVLLVEHDVSLVMGLVDRILVL
                     NYGEQIAEGTPAQVRADPRVIAAYLGDDEVAS"
     gene            complement(3752..4648)
                     /locus_tag="AC159B_04580"
     CDS             complement(3752..4648)
                     /locus_tag="AC159B_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR3377055.1"
                     /translation="MTLSQQIAQFIASGIINGTIYALLGLGLVAIHSVTRVVNVAQGE
                     FAAFGALVAVSLVAAGLPLAVSLVAAIVAAALLGGVIHRVAIRPARDATPLVLLIITI
                     AVHLALKGVLLLLWGTDPYTLRPFTVGPPIRILSAVLTRQSLWVIGLTAVALVILYLF
                     FTQTIRGKSLRACAVNPTAARLVGIRVEWMGTLAWTIAGGLGGLAGVLIAPLTLATYD
                     MGLILGLKGFVGAVVVGMASYPWTVVACILFGMVESLGTGLISSNYRDAIAFGALIVV
                     LLWRAIATQRAGWIVSEEVAQE"
     gene            4729..5142
                     /locus_tag="AC159B_04585"
     CDS             4729..5142
                     /locus_tag="AC159B_04585"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF046335.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HIR3377056.1"
                     /translation="MKQGLQPGAEATVTITVTADMLATFEELGPVHPLYSTWSMVRHM
                     ELASRKIILPFLEPGEDAVGHSINVTHTAPTGIGERVTARARLIEIDGRRIVCAVEAH
                     NEHERIGEGTNVQMLVRKDRLALLIEQLQRRVGAR"
     gene            5139..6095
                     /gene="dapA"
                     /locus_tag="AC159B_04590"
     CDS             5139..6095
                     /gene="dapA"
                     /locus_tag="AC159B_04590"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HIR3377057.1"
                     /translation="MSPQFKGSLVPVVTPFHGGQFDERRFVELLDWQIENGSHGVVIA
                     GTTGEPGALSLEEREHLIDVAVRTVRRRVPVIAGTGTTNNAETLRLTRFAKKAGADAS
                     LVVVPYYVRPTQDGLYRHFRSIADQVDTPIIIYNIPGRTAVNMEPQTMAALARDCRNI
                     IGVKEANKDFEHINRVLHACGRSFLVYSGIELLCFPVLAIGGAGYVSATGNVLPKDVA
                     RLYDLAAAGKWDEARDLHYRLLPMNDALFLETNPVPAKTALGLMGKIDPEVRLPLAPM
                     APENVQKLKQVMTSYGLLVEPALNHSLRSGSGQARAGGSSAP"
     gene            6092..7603
                     /gene="hpaE"
                     /locus_tag="AC159B_04595"
     CDS             6092..7603
                     /gene="hpaE"
                     /locus_tag="AC159B_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013987341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HIR3377058.1"
                     /translation="MSVERAPRVVVGPARHFIDGEFVGGAEGRTFATLNPATNEPLTQ
                     VAEGGQTDVDRAVRAARKAFDTGPWPRMAPADRARALTRVADAIDHHVDALALMETLD
                     TGIPISQVRKGQIPRAADNFRFFAEMATRMTGETYPVGETFVNYTVRQPVGVAGLITP
                     WNTPFMLETWKVAPCLAAGNTCVLKPAEWAPVSAQELAEVIQAADLPPGVFNVVQGFG
                     ETAGAALVAHPQVQLISFTGETTTGKEIMRNGAATLKRFSMELGGKSPVVVFADADLD
                     RALDATIFGLFSLNGERCTAGSRLFLEEPIYEAFVDNVVRRAANIRIGDPQEQTTELG
                     PLIHPEHWTRVSGYIEVGLKEGARVATGGKRPERFPRGNYLEPTVLVDVNNGMRVAQE
                     EIFGPVLVTIPFRDESEVVRMANDTRYGLVAYVWTRDASRAHRLASGVDAGMVWINSQ
                     NVRDLRTPFGGMKESGIGREGGHYSFDFYTELKTVHVALGAHHIPKMGTGGQA"
     gene            7603..9117
                     /gene="hpaB"
                     /locus_tag="AC159B_04600"
     CDS             7603..9117
                     /gene="hpaB"
                     /locus_tag="AC159B_04600"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HIR3377059.1"
                     /translation="MPARTGSQFIEGLRGQSREVWIGGERVKDVATHPAFRNVTRSLA
                     ALYDMQHDRATREEMTYVSPSSGERVGLSFLTPRSRDDLSRIRRMMKQWADYSGGMMG
                     RSPDYLNRAVMAFAAAAEYCGQNDPRFADNVRRYYELVRERDLCLTHTLINPQANRAA
                     GPAGQGDPHLAAGVVKETERGVLIRGARMLATLPIADEIMVFPSTLLRGAEEDRPYAF
                     AFAIPCGIPGLKFICRETFDAGGNAFDYPLTSRFEEMDAVVVFDDVLVPWERIFLLGD
                     VERANKAFSATGAVAHMAHQVVTKNIAKTEFILGVASLIVDAIAIEQFQHVQGKIAEI
                     IYYLESMKAYIRASEADAVVDRWGVMTPAWPPLDAARNMFTWMYPKMIEIVQLLGASG
                     LMARPTNADVEGPLGAVIEKYYQAARAGAVDRIKLFRLAWDVALSSFGSRQVIYERFF
                     FGDPVRMQMATFGSYDRQPYMKRVREFLAREDGVERSGPVTAEPVRGGSDSSTQ"
     gene            9117..9710
                     /locus_tag="AC159B_04605"
     CDS             9117..9710
                     /locus_tag="AC159B_04605"
                     /inference="COORDINATES: protein motif:HMM:NF013756.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR3377060.1"
                     /translation="MTDPSPPVTPAAFRKVMGLFATGVTVVAASEGDEIHGMTANAVT
                     SVSLDPLLVLICVDKRARMNRFLESAGGFSINILSEDQEALSRYFAGSWKPPGPPEFR
                     FHPWTGGPRLVGCMAAVGCAIDRTIEAGDHRIVLGRVMALHEGDVSSRPLLFFGGRYR
                     RLEELEAPVPPEEWSHEAVHIYYDEWSGTTRQSGEEP"
     gene            9710..10702
                     /gene="hpaD"
                     /locus_tag="AC159B_04610"
     CDS             9710..10702
                     /gene="hpaD"
                     /locus_tag="AC159B_04610"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HIR3377061.1"
                     /translation="MDRSAQILRLGHVEYRVTDLERARAFYVDLLGFVETARDRGRLY
                     LRGLEEREHHSFVLRKAASAGVSHFAFRVGGEDDLDRLQRRCESAGRPTRWVAAGEDE
                     GQGRALRVQDAGGLPVEFYHEMAPVDRHLQRFDLYRGPHVMRIDHVNCQVRDVQGAYD
                     WYTREWGFWCSEYTETDDRPPRLWAAWLHRKQNVHDIALMNGIGPRVHHAGFWVPDAL
                     AVLRACDILAAAGEAGAIERGPGRHGLSNALFVYLRDGDRNRIELYAGDYLIADPDWK
                     PIRWSINDPRRATFWGHVPPKSWFDEASLVESITDGALIEPQAPQLQDRPSFVT"
     gene            complement(10675..11625)
                     /locus_tag="AC159B_04615"
     CDS             complement(10675..11625)
                     /locus_tag="AC159B_04615"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HIR3377062.1"
                     /translation="MRIAAIGAGGTGGYFGAQLQRSGQDVAFVARGAHLSAMRSQGLR
                     VESAAAEPLVLRVRATDRPAEIGPVDLVLFTVKSYDTVTAAKLLPPLIGKDTAVVTLQ
                     NGVDNIDLLLEAVGAQRVMGGLCRIFSTIASPGVIRQTGGPRSIIFGELDGEVTDRAG
                     AILEVFRAAHIPTELSRQILVDMWEKYVFITAQGGMTALARVPIGQILATPETREMYL
                     DVASEVAAVGRAHGVSIAPGERDRVLKFAQALEPGLYSSLHHDLTHGNRMEVEALPGN
                     VVRLGRRYGIPTPVCRVVYAALLPHEVAAREGQVTKLGRS"
     gene            complement(11699..11935)
                     /locus_tag="AC159B_04620"
     CDS             complement(11699..11935)
                     /locus_tag="AC159B_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3377063.1"
                     /translation="MDLMEHDLVILESQADVAAVVAAAQKLGYEASTYPRSSEKANDT
                     SWVVTTRPRRETSVVSRQAPSSQPSQVSVEVGGR"
     gene            complement(11996..12211)
                     /locus_tag="AC159B_04625"
     CDS             complement(11996..12211)
                     /locus_tag="AC159B_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3377064.1"
                     /translation="MLTGNRLKPAQVNHLRSNRASVNLSPDAPSTLMEMVGIVLDPQI
                     YRAVPKASGAVQNRRPRLLIKQPAYTF"
     gene            complement(12319..13254)
                     /locus_tag="AC159B_04630"
     CDS             complement(12319..13254)
                     /locus_tag="AC159B_04630"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR3377065.1"
                     /translation="MLLILTSEAPAAQPVGSSYTYITIREGQTLWSLARAYGISVELI
                     AELNGLRSPEAIRVGQRLKIPVQTTQTPRPAPRVTSMAARHQFVWPARGMLTSRYGWR
                     RRRHHSGIDIGADRGAPIVAAGNGVVVYAGWYHAYGRTVIIEHGNGWTTLYGHASSIG
                     VRPGQSVSAGQLIARVGCTGRCTGPHLHFEIRVNGRAMDPLSVSLLDPLQAPAKDSTT
                     AERAAAGRAAPVSVETVVHRSMTADGPGGHIIQNVEDTLRDGKVVRRVEVTTKVEGGH
                     IVRLRKTYGLVNGELQLRSETREVVDTTPERQRQP"
     gene            13531..13606
                     /locus_tag="AC159B_04635"
     tRNA            13531..13606
                     /locus_tag="AC159B_04635"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13564..13566,aa:Ala,seq:cgc)
BASE COUNT         2636 a         4287 c         4344 g         2455 t
ORIGIN      
        1 gttgggccct cccgggccct catctatcgc gcaggcgccc agttattgtt ccgaacctca
       61 atcatgaccg cagcgcgtcg gtccagaccc aggtggtctt tggctgtgaa agtaaagatg
      121 cccgtgatcc ccaccagctc ttttgtgccg cgctcgatct gatcacggag tacggagcgg
      181 aacccaccga tgttatccgg cccgataccc tgtcgcagcg tacgctcgat cgccggctgc
      241 aggatcagca tagcgtccag cgcatgccca ccgaacgtat tacgcgttcc tctcccatac
      301 cgtccctcgt agcgctccag ataaccgagc agtgcacgct tttgcttgtc ggcttccggg
      361 agaagttcgg cgatcaacag ctttcctgcg ggaagcagcg tgccctcgac gttgggtcct
      421 ccgaggtcca ggaacgtctt gttcgcgacg ccgtgactct ggtagacggg caagttgatg
      481 ccaaggtccc gaatgttccg ctgagagacg ttggctcccg gaggtatggc ccagatcaga
      541 aatgcttctg ggttacgccc catcgcccga gtgatctgcg tgctcacatc cgtcgccgtg
      601 cgggcaaacg attctctcgc cacgatctcg atgcccttct gcggcgcaaa cttgtcgaac
      661 tcccccaatc ctacctgacc gaatgcgtcg tcgaagccca gccatgcgac gcggcggata
      721 ccgcgctggc gcatgtggtc cgtgagcacg tcgatcatca agcggtcggt ctggggagtt
      781 ttgaaaatcc accgccgctc ctcaacaggc tcaatgatgg tgaccgcggc cgcgagtgag
      841 acgttgaccg tcctcgcggc ctgcaccgtt tgaagaatgg ccaggctggc cggacttgtc
      901 gtgcagcaga cgacggcgac gacccgatct tcttcaatca accgcttcgc cgcgatcaca
      961 gccttcgtgg gatcgctctc tccgtccgtc acaaggtatt ggatccgaac cggtcccgcg
     1021 ggaccttgta tggtggcgaa gtcatcctga tacatcagca ccgtattgcg ctcgggcacg
     1081 cccagtgaag aggccgggcc cgtgatgtcg acaacgaacc ccactttaac cgtctgctgc
     1141 tgcgcagccg tcgggagcgc gagcaaggtg atcacagcaa acacgatcaa cacacgttgc
     1201 ataaccacac ctccacgttc atcgttcctg tgcgggctcg acgtggccgc cgagataggc
     1261 agcctgtacc ttagggtctt cgccgagttc tgccgccctc ccggcgagca caacgcgccc
     1321 accctccata acgtatgcgc gatccgcgag cgccagggcc gcctttgcgt tctgttccac
     1381 gatcaggatg ctttgtccac gggactgcag ctcccgcagc gtccgcacga tctctttgat
     1441 caccagcggg gccaaaccca ggaggggttc gtccatcagt aacagccgcg gcctggccat
     1501 caagccccgt ccgatcgcca gcatctgctg catcccaccc gacaacgtca tcgccggatg
     1561 cccggcgcgt cctttcagga cctggaagag ttcaaacacc tcacggatgt catgctcgat
     1621 agcgtttgcc gtctcggatc cgaaccgatg atacgcgccc aagagaaggt tgtcgcgaac
     1681 gctcatcgat ccaaagagtt ggcggcgttc ggggaccagg ctcatcccga gacgcaccac
     1741 gtcctcgggc gcgatgccgt cgatgtgccg gccggcaaaa gtgatcacgc cggctgcggg
     1801 gaccaagccg gccagagctg acaggagcgt agttttgccg gcgccgttcg gaccgataat
     1861 cgcgacgacc tcgctctcgg cgacggtcag cgagacatcc cggacggcct gcacgggacc
     1921 atacgatgcg gcaaggtgct cggcctgaag cagcaaggtc acgaagcgac ctcgtcgtca
     1981 ccgaggtacg cggcgatgac acgaggatct gcccgaacct gggcaggggt gccttccgcg
     2041 atctgctctc catagttcag caccagtatt cggtccacaa gacccatgac aagcgagaca
     2101 tcgtgttcga cgagcaacac cgtgatgccg gcgtctcgga tctgccggat caggcggacg
     2161 agttccccgc gttcagacgt cgacaaaccg gaagccggtt cgtcgagcag cagtaatgcc
     2221 ggctgggccg ccagggcacg ggccagttct aaaaaccgct gtgcgccgaa gggcaaggcg
     2281 ccgcttctgt ccatggcgcg gtccagcaat cccacacgct cgagatagcc cgccgcgtcg
     2341 gccgctgccc ggcgatcctc tcggcggccc aatcccagca ttgagcccag aaatccggtc
     2401 ccgaggcttc ggtgcatgcc aatcatcacg ttgttcagca ccgtcaggtc gccgaagact
     2461 ctgggggtct gaaaggtgcg cgcaacgcca agtccggcga tagcataggg ttgcagacgt
     2521 gtgatggttt gtcccttgaa tgcaatgtcg ccgctcgttg cgcgcaacac gccggagatc
     2581 aggttgaatg ccgtcgtctt gccggccccg ttcgggccaa tcatcgcgta gatttctccc
     2641 tgcttcacct cgaaagacag gccacttact gcgagcaggc cgccgaagcg tttttgaagc
     2701 ttatcgactt tgagcagagt ctctccacgt tggagagaga gcgaaggtga gggggccaac
     2761 ccggctgcca cagactcttg gcggaactca ggccgctttg cattgagacg gaagactgca
     2821 gcaacccagg gccagatgcc gcccggcgcc agcacgacga ccacggcgag gagcagtcca
     2881 aatatgacga tctcgtactc cgccgacgcc ccggttccga ggagtcgagg catgatcgtg
     2941 cgcaggacct cgcgcagcac ggtcaggacc gcggtcccaa gcaccgcgcc gggcagaaat
     3001 gcgatgccgc cgaggacgct catcaccagg aggtcgatcg agaagttgaa cgcaaaggga
     3061 gaggggctga cgaagccgag atagtggaca taaatggcgc ctgcgaggcc ggcgaacccg
     3121 gcgctgagcg cgagcatgcg cgccttgaag aacgcaggat tgatcccgag tgttgcggcc
     3181 gttacttcgc tcgcccggat cgcagccagc gcccgtccga tccgggagtg gatgagattg
     3241 cgtccaagcc acagcgccag cagcaccagg gtccagatca agtagtaata ggcccgatcg
     3301 cccacgatga tgagtctgcc gaccgacaac ggcgggatgc cggtcaggcc tgtggggccg
     3361 ccggtcagac cctgcacgtt gcggattacg acatcgacga cgatgttgaa tccgagcgtc
     3421 ccgagcacca gaaagtgccc ccgcagcaga aagatcggag cgccgatcac gtacgcgata
     3481 aatgcggcgc cgctcacggc cagcatcact cccaggaacg gcggcagacc cagccgcagt
     3541 gtcgtaatcg cagagacgta cgcgccgatc ccgtagaatg ccgcctggcc gagagaaact
     3601 tgtcccgcgt ggcctgccag cagggtcagc ccgatgacga tcagactgaa caggccgatg
     3661 aagatgaaga tgccgagact gacgcgaccg gccacgagac ttggcgtgat gatgaggaca
     3721 atgatgagcc caagaagcca cgcgcgctgc gtcattcctg ggccacttct tcactcacga
     3781 tccagccggc ccgctgtgtc gcgatggccc gccacaggag cacgacgatc aacgccccaa
     3841 acgcgatggc atcacggtag ttcgatgaga tcaggcccgt gccaagcgac tccaccatgc
     3901 caaagagaat gcaggcgacc accgtccacg gatagctggc catccccacg accactgcgc
     3961 ccacgaatcc tttgaggccg aggatgagcc ccatatcgta ggtcgccagc gtcagcggag
     4021 caatgagcac gccggctaaa cctccgaggc cccccgcgat ggtccacgcg agcgtgccca
     4081 tccactccac tcggatcccc acgagcctcg ccgcggtcgg attcaccgcg catgcccgga
     4141 gcgacttgcc tcggatcgtc tgcgtaaaga agagatacaa gatgaccagg gcgacggccg
     4201 tcaggccgat cacccagaga ctctgccgcg tcagcactgc ggacaaaatg cggatcggcg
     4261 gtcccacggt gaagggccgg agcgtgtatg gatccgttcc ccagagaagc agcagcacgc
     4321 ccttgagggc aagatgaacg gcgatggtga tgatcagcag gaccagtggc gtcgcgtccc
     4381 gggcaggacg gatcgcaacg cgatgtatga cccctccgag cagggcggca gctacgatgg
     4441 cagccacgag cgacacggct aagggcaagc cggccgccac cagactgacc gccacgagcg
     4501 cgccgaaggc ggcaaattct ccctgggcta cgttcacgac tcgcgtcacg ctgtgaatgg
     4561 ccacgagtcc caaccccaag agcgcgtaga ttgtcccgtt gataatgcca ctggcaatga
     4621 actgagcgat ttgctgggag agggtcatgg tgaagtgaag agggcttcac cgtgcctcag
     4681 aaccactcct cgcaggacca cttatcctcc gatagaacgc tgtacaatgt gaaacaagga
     4741 ttgcaaccgg gcgcggaagc gaccgtcaca atcacggtga cggcggacat gttggccacg
     4801 tttgaggaac tggggcctgt ccacccgctc tactccacct ggtccatggt ccggcatatg
     4861 gagctcgcga gccggaagat catcctgccg tttctcgagc ccggagaaga cgcggtcggt
     4921 cacagcatca acgttaccca tacggcgccc accggtattg gagagcgcgt cacggctcgg
     4981 gcacggctga tcgaaattga cgggcggcgg atcgtgtgcg ccgtagaggc gcacaacgaa
     5041 cacgagcgga tcggggaggg cacaaatgtg cagatgctcg tgagaaagga caggctggcc
     5101 ctgctcatcg agcaactcca gcgccgtgtg ggcgcgcgat gagccctcag ttcaaaggct
     5161 ccctcgttcc cgtcgtcacc ccattccacg gtggtcagtt cgacgaacgt cggtttgtcg
     5221 agctgctgga ttggcagatc gagaacggca gccacggcgt cgtcatcgcc ggcacaaccg
     5281 gcgagcccgg cgcactaagc ctggaagaac gcgagcacct gatcgacgtg gccgtgcgta
     5341 ctgtgcgccg ccgagtaccg gtgatcgcag ggaccggaac gacgaacaac gcggagacgc
     5401 tacgcctcac caggttcgcg aagaaagccg gtgcggacgc ctcgctggtg gtcgttccct
     5461 actatgtcag gccgacgcag gatgggctgt accggcactt ccgcagcatc gccgaccagg
     5521 tcgacactcc gatcatcatc tacaacatcc cgggacggac ggcggtgaac atggagcctc
     5581 agacgatggc cgcgctcgcc cgggattgcc ggaacatcat cggcgtaaaa gaagcgaata
     5641 aggattttga gcacatcaac cgtgtgctgc acgcttgcgg ccgttcgttc cttgtctact
     5701 ccggtatcga gttattgtgc ttccctgtgc tggccatcgg cggcgcggga tacgtgagcg
     5761 caaccgggaa tgtgctcccc aaggatgtgg cgcgtctcta tgacctcgcg gcagccggca
     5821 agtgggacga ggcacgtgat ctgcattatc gcctgctccc gatgaatgac gcgttgttcc
     5881 tcgagacgaa cccggtgccg gcaaaaacgg cgctcgggtt gatggggaag attgaccccg
     5941 aggtgagatt gcccctcgcc cccatggccc ccgagaacgt gcagaaactg aagcaggtca
     6001 tgacctcgta cggtttgctc gtcgagcccg ccctaaacca ctcactccgt tcgggttcag
     6061 ggcaagcccg agccggcgga tcgagtgcgc cgtgagcgtt gagcgcgcac cacgtgttgt
     6121 ggtcggcccc gcccgccact tcatcgacgg agagtttgtc ggcggtgcgg aaggccgcac
     6181 gttcgcgacc ctgaatccgg caaccaatga acccctcaca caggtcgccg aaggtggaca
     6241 gacggacgtc gaccgggccg ttcgcgctgc gcggaaagcg ttcgacacag gaccgtggcc
     6301 gcggatggct cccgccgacc gggccagagc actgacgcgg gttgccgatg ctatcgatca
     6361 tcacgtcgat gctctcgccc tcatggagac gctggatacg gggatcccca tcagccaggt
     6421 gcggaagggg cagatcccgc gggcggccga taacttccgc ttcttcgcgg aaatggccac
     6481 gcgaatgacg ggtgagactt accccgtggg cgagacgttc gtcaactaca cggtccggca
     6541 gcccgtgggt gtcgcggggc tcattacacc ctggaacact ccgttcatgc tcgagacatg
     6601 gaaggtcgcg ccgtgccttg cggcggggaa cacgtgcgtg ctgaagccgg ccgagtgggc
     6661 tccggtcagc gcgcaggaac tggcggaagt cattcaagcg gccgatctcc cccctggtgt
     6721 tttcaatgtg gtgcagggtt tcggtgagac cgccggggca gccctcgtgg ctcatcccca
     6781 ggtccagttg atttcgttta ccggtgaaac caccaccggg aaggagatca tgcgcaacgg
     6841 cgcggccacg ctgaagcggt tctcgatgga gcttgggggg aaatcgcccg tggtcgtctt
     6901 cgcggacgcc gatctcgatc gtgcccttga cgccacgatc ttcggattgt tctcgctgaa
     6961 cggtgagcga tgcacggccg gctctcgctt gttcctcgaa gaaccgatct acgaggcgtt
     7021 tgtcgacaac gtcgtccggc gcgcagcgaa catccggatt ggagacccac aagaacagac
     7081 gacggaactg ggtcccctga ttcatcctga gcactggacg cgtgtttccg gctatatcga
     7141 ggttggcctc aaggaaggtg cgcgggtcgc caccggcggc aaacggccgg agcggtttcc
     7201 gcgcggcaac tatctcgagc ccacggtcct cgtggatgtc aacaacggga tgcgtgtcgc
     7261 gcaggaggag atctttgggc cggtgctcgt gacgatcccg tttcgagacg agtccgaggt
     7321 cgtccggatg gccaatgata cgcggtacgg cctcgtggcc tacgtgtgga cgcgtgacgc
     7381 ctcgcgcgcg caccggctgg catcgggcgt tgacgccgga atggtctgga tcaattcgca
     7441 gaacgtccgg gacctccgta cgccgttcgg agggatgaaa gagagcggga tcggccgcga
     7501 aggcggtcac tacagcttcg atttctatac ggagctgaaa accgtccacg tcgcgcttgg
     7561 tgcgcaccac attccaaaga tggggaccgg ggggcaggcg taatgcccgc tcgcacgggc
     7621 tcgcagttca tcgaggggct gcgcggtcag tcccgagagg tgtggatcgg cggcgagcgc
     7681 gtcaaggacg tcgccacgca ccccgcgttc aggaacgtca ctcggagcct cgcggccctg
     7741 tatgacatgc aacacgaccg cgctactcgg gaggagatga cgtacgtctc accctcgagc
     7801 ggggagcgag tcggactttc gttcctcacg ccgcgctcgc gcgacgacct cagccggatc
     7861 cggcggatga tgaagcagtg ggcggattat tcgggcggca tgatggggcg ctcacccgac
     7921 tacctcaacc gggccgtgat ggcgttcgcc gccgccgctg agtactgcgg ccagaacgac
     7981 ccgcgttttg ccgacaacgt caggcggtat tacgagcttg tacgggaacg cgatctctgc
     8041 ctgacgcaca cgctgatcaa tccccaggcc aaccgcgccg ccggccccgc ggggcagggc
     8101 gatccccacc tcgccgcggg ggtcgtgaaa gagacagaac gcggcgtgct gattcgcgga
     8161 gcgcgaatgc tggctaccct gccgatcgcc gacgaaatca tggtctttcc gtcgacgctg
     8221 ctgcggggcg ccgaagagga ccgtccatat gcctttgcgt ttgcgattcc ttgcggcatt
     8281 cccgggttga agttcatttg ccgcgagacg ttcgatgccg gtgggaacgc atttgactat
     8341 ccgctgacct cacggttcga ggagatggat gccgtcgttg tcttcgacga cgtgctggtc
     8401 ccgtgggagc ggatctttct gctgggcgac gtggaacggg cgaacaaggc gttctcggca
     8461 accggggccg tggcgcacat ggcccatcag gtcgtgacca aaaacatcgc caagaccgag
     8521 ttcatcctcg gagtcgcgtc gctgatcgtc gatgccattg cgattgagca gttccaacac
     8581 gtccagggga agattgcgga gatcatctac tacctcgaga gcatgaaggc atacatccgt
     8641 gcgagtgagg cagacgcggt cgtggaccgg tggggggtga tgacgccggc ctggccaccg
     8701 ctcgatgcgg cgcgcaacat gttcacatgg atgtatccga aaatgattga aatcgttcag
     8761 ctgcttggcg cgagcgggtt gatggcccgg ccgacgaacg ccgacgtcga gggacccctg
     8821 ggtgccgtga ttgagaagta ctaccaggcc gcccgtgcag gcgcggtgga tcgcatcaaa
     8881 ctgtttcggc tggcctggga tgtggccctg agctcgtttg gatcacggca ggtgatctac
     8941 gagcgcttct tcttcggcga cccggtgcgc atgcaaatgg cgacgttcgg ctcctacgac
     9001 cgccaaccct acatgaaacg ggtccgcgag ttccttgcac gtgaggacgg ggtcgagcga
     9061 tccggaccgg tgacggcaga gcccgtgcgc ggcggctccg attcttccac gcagtgatga
     9121 ccgacccttc gcccccggtc acgcctgccg cgttccgaaa agtcatgggc ctctttgcga
     9181 cgggagtcac cgtggtcgcc gcaagcgagg gcgacgagat ccacggcatg acggcgaatg
     9241 ccgtcacgtc ggtctcactc gaccctctgc tggtgctgat ttgtgtggac aagcgcgcgc
     9301 gcatgaaccg gtttctggag agcgccggcg gattctcgat caatatcctc tccgaagatc
     9361 aagaggcact ctcgcgttac ttcgcgggtt cctggaaacc gccggggccg cccgagtttc
     9421 gatttcatcc gtggaccgga ggcccgcgcc tcgtcggctg tatggcggcc gtcggctgcg
     9481 cgatcgaccg gacgattgaa gctggagacc accggattgt gttgggcagg gtgatggcgc
     9541 tgcacgaggg cgatgtgagt tcgcgcccgc tgctgttttt tggaggtcga tatcgccggc
     9601 tggaggaact ggaagcgccc gttcctcctg aagagtggag tcacgaagcc gtgcacatct
     9661 actacgacga gtggtcgggc accacgcggc agtcgggcga agagccgtga tggatcggtc
     9721 cgcgcagatc ttgcggctgg gacacgtcga gtatcgcgtc actgatcttg agcgcgcccg
     9781 ggcattctac gtagatctgc ttgggtttgt cgaaacggcg cgcgatcgag gacgcctgta
     9841 tctccgtggc ctcgaggagc gagagcacca cagctttgta ctgcgcaaag ccgcatcggc
     9901 aggggtgtcg catttcgcgt tccgggtggg gggagaggac gatctcgacc gtctgcagag
     9961 gcggtgcgag tcggcaggca gaccgacgcg gtgggttgcg gccggcgagg acgaagggca
    10021 ggggagggcg ctgcgcgttc aggacgcagg cggactgccg gtagagttct atcacgagat
    10081 ggcgcccgtc gaccggcacc tgcagcgatt cgacctatac cgaggtcccc atgtgatgcg
    10141 catagaccac gtaaactgcc aggttcgcga tgtgcaggga gcctacgact ggtacacccg
    10201 ggaatggggg ttctggtgct ccgaatacac cgagaccgat gatcgaccgc cgcgattgtg
    10261 ggcggcgtgg ctgcaccgca agcagaacgt gcacgacatc gcgctgatga acgggatcgg
    10321 gccccgggtg caccatgcag ggttctgggt acccgacgcg cttgcagtgt tgcgcgcgtg
    10381 cgacattctc gcggccgccg gggaagcggg tgccatcgag cgtggtccgg ggcgccatgg
    10441 actatcgaac gcgctcttcg tgtaccttcg cgacggtgac cggaacagga tcgagctgta
    10501 cgcgggcgac tacctgatcg ccgatccaga ctggaaaccg atccgctgga gcatcaacga
    10561 cccgcgccgc gcgaccttct ggggccacgt gccgccgaag tcctggtttg atgaagcatc
    10621 gctggttgag tcaattacag atggagcact gattgagccg caggcaccgc agcttcaaga
    10681 ccgcccgagt ttcgtgacct gaccttctcg cgcggcgact tcgtggggga gtagcgcggc
    10741 gtatacgaca cggcaaacag gcgtcgggat gccgtagcgt cgccccagtc tcaccacgtt
    10801 tcccggcaac gcctccactt ccatacgatt tccgtgcgtg agatcgtggt ggagcgagga
    10861 atatagaccc ggttccaacg cctgagcgaa cttcagcacg cggtcgcgct cccccggtgc
    10921 aatcgagaca ccgtgcgccc gacccaccgc ggcaacctcg gacgcgacat ccagatacat
    10981 ctcccgtgtt tccggggttg caaggatctg tccgatgggc acgcgggcca acgcagtcat
    11041 gcccccctgc gcggtgatga aaacgtactt ctcccacatg tccaccagga tttggcgcga
    11101 cagttctgtc gggatatgcg cggcgcgaaa aacctcgagg atggccccgg cacggtccgt
    11161 gacctctcca tctaactctc caaagatgat cgatcgtggc cctccggtct gacggataac
    11221 gccgggggag gcaatcgtcg agaaaatccg gcagaggccc cccatgacgc gttgcgcgcc
    11281 gacggcctcg aggagcagat cgatgttatc cacaccgttc tgcaaagtca caacagcagt
    11341 atccttgccg atcaatggcg gaagcagttt tgcggcggtc actgtatcgt acgacttcac
    11401 ggtgaagagt acgaggtcaa cgggtccgat ctcggcggga cggtccgtgg cgcgaacgcg
    11461 gagcacgagc ggttcggcgg cggccgactc gacgcgcaga ccctgggagc gcatcgcgga
    11521 gagatgcgcg ccgcgcgcga cgaacgcgac gtcctgtccg ctgcgctgca actgggcccc
    11581 gaagtagcct ccggtgcctc cggccccgat cgccgcgatt ctcattcttc catcataaat
    11641 gttgtcggcc acccgtttcc gggtggccgg tgttgcacag tgtgggtctg gttatgcctt
    11701 agcggcctcc gacctcaacc gaaacttgcg atggctgtga ggatggcgcc tggcgagaga
    11761 cgaccgacgt ttcacgacgc ggcctggtcg tcaccaccca ggaagtatcg ttggcctttt
    11821 ccgaggaccg cggataagta gaggcctcat agccgagttt ctgggccgcg gcgaccactg
    11881 cggcgacatc tgcctgggat tcaaggatga ccaggtcgtg ttccattaaa tccatcaacg
    11941 cacatttcaa atacaggcga tccatgcgga agtcccccgt ttcagttgtg ggtgtttaga
    12001 aggtgtacgc cggctgctta atcaagaggc gcggtcgccg attctgcacg gcgccggatg
    12061 cctttggaac agctcggtag atctgcggat ccagcacgat gccgaccatc tccatcaggg
    12121 tggatggagc atcaggcgac aggttgacgc tcgccctgtt gctcctcaga tgattgactt
    12181 gcgcaggctt cagacggttc cccgtaagca tgcatacctc ccccaagttc cggtggtttg
    12241 atttacccca caagaacacc ggcctaacat cagccagggg cttatcaggg gagattgctt
    12301 agttggccta ccgggggact aaggctgtcg ctgacgttcg ggggtggtgt cgactacctc
    12361 acgcgtctcg cttcttagct ggagttctcc attcaccaac ccgtaggtct tgcgcaatcg
    12421 aacaatgtgg ccgccttcga ccttcgtcgt gacctcgacg cgccgcacga ctttaccatc
    12481 acggagcgta tcttcgacgt tttggatgat gtgtccacct ggtccatccg ctgtcatcga
    12541 tcggtgaacc acagtttcga cactgaccgg cgccgcccga ccggccgctg cccgttccgc
    12601 ggtcgtggag tctttcgcag gagcttgaag tgggtcgagg agcgagaccg agagcggatc
    12661 catcgcccgt ccatttacgc ggatctcgaa atgcaggtga ggaccagtgc accgtcctgt
    12721 gcatccgact cgagcgatga gctgaccggc ggagacactc tggcctgggc gaacgcctat
    12781 cgaggaagca tggccgtaca gcgtcgtcca tccgttgccg tgctcgatga taaccgtgcg
    12841 cccgtaggca tggtaccagc ccgcgtacac gacgaccccg ttcccggcgg cgacgatagg
    12901 ggcgccgcga tctgctccga tgtcgatgcc gctgtgatgc cggcgccgtc tccacccata
    12961 ccgggacgtc agcatgccgc gggccggcca cacaaactgg tgccgggcgg ccatgctcgt
    13021 gacgcgcggt gccggccggg gggtttgggt cgtctggaca ggaatcttca gcctctgccc
    13081 aacgcggatc gcctcggggc tgcgtaagcc gttcagttcc gcaatcagct caactgaaat
    13141 gccatatgcg cgcgcgagag accacagggt ctgaccttca cggatcgtga tgtaggtata
    13201 cgaagacccg acgggttgtg ccgccggcgc ttctgaggtg aggattaaca acatgaggag
    13261 agcagcgaga agtcctgcgc cccgaaagcc tgccgtctct cgcattcgcc tgtgccccgc
    13321 cggactgccc ctcgatcggc cggcagccaa gaagatcgag ccccccgact cgtcgccgac
    13381 atcacgagcc tgccccgaat ctcatgcgcg cagactacgg gtcccctttc tccaaggtcc
    13441 ccatcccgcc gcgtgttcac taagacaccc tcgtgatctg agcggctgtt gatgggctcc
    13501 gtaagggtcg cgtataatac tcctgttcgt ggggacgtgg ctcagttggg agagcgcctc
    13561 gttcgcaacg aggaggtcgg gggttcgact cccctcgtct ccaccatgaa tggcgactgg
    13621 tccgtcttgc gggtcggtcg ccattcacgt ctgcgaacag agaaccgaac ccccgacctg
    13681 agggggagcg ggggaggtac tcccccagat agtaaagggg gc
//
LOCUS       DBNGSB010000160         2345 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2106932, whole genome shotgun sequence.
ACCESSION   DBNGSB010000160 DBNGSB010000000
VERSION     DBNGSB010000160.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2106932"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="AC159B_04640"
     CDS             complement(<1..441)
                     /locus_tag="AC159B_04640"
                     /inference="COORDINATES: protein motif:HMM:NF021489.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HIR3377066.1"
                     /translation="MFPKTWLTMRRLLPWVILFVLLAVCGPAWAKSYDHPLIEQTFRL
                     LPTGDADVQEIRTFRFDGSFTFATITRSTRGQYGSYGLEYAGVWAAATNPRLRFQVER
                     SGDDVTLRWSYAAENETKRFLIRYRITGAVQRYQDVAQFYWQAVE"
     gene            552..1673
                     /locus_tag="AC159B_04645"
     CDS             552..1673
                     /locus_tag="AC159B_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015143687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR3377067.1"
                     /translation="MAGRTRREFLKTAVAAGVGAATATKFKTINIVTAQAPIVWKMQS
                     GWPTRDFFHESAIETSKKFSEMSGGRIRVDLLPAGSVVGAFGIIDAVHSGLLDAGHAV
                     GVYAYSKSPTTSLFGTGPSFGMDATDMLSWFYYGGGFDMYQDLLQNQLKLNVTAFMMG
                     PMPTQPLGWFRNPILKADQLKRTKFRTVGLSAELFAEMGASVVILPGGEIVPALERGV
                     IDAAEFNNPSSDKALGLPDVRKVYMLQSYHQPVETLEVTINKGKWDALSAELKAIVRG
                     AILAESADYHYRAIHRNSQDLEEMERRGIRVFATPREILLAQLNAWDKIVAKRSKEDA
                     NFARIVSSQRTFARRVVRWKQRIYVDNSIAFNRYFRASK"
     gene            1758..2294
                     /locus_tag="AC159B_04650"
     CDS             1758..2294
                     /locus_tag="AC159B_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HIR3377068.1"
                     /translation="MQKWIKRIDRLSEIVGTSAAWLVVIMTLAMSTEVLSRRLLGRPT
                     TWAFDVSYMSYGAYFFHGSAYTLAKNAHVRGDIFYRNFSPRTQAIIDFSLYLLVFFPA
                     MIAMVVIGADYFWESYRIRETSPLSPYDTPVYPLKAVIPIGAFLLLVQGVAQVMRTII
                     AIRTNRWHDQEREAEVVA"
BASE COUNT          469 a          714 c          709 g          453 t
ORIGIN      
        1 ctccaccgcc tgccaataga actgggccac gtcctgatat cgttggactg cgcccgtgat
       61 gcggtaccgg atgagaaacc gttttgtctc attctcggcg gcgtaggacc accggagtgt
      121 gacgtcatct ccggatcgct cgacctgaaa gcgaagtcgc ggatttgtcg ccgcagccca
      181 cacccccgcg tattcgaggc cgtaggaccc gtattggcct cgtgtgctcc gcgtaatcgt
      241 ggcgaaggta aacgatccgt cgaaccggaa cgtgcggatc tcctgcacgt cagcgtcacc
      301 ggtgggcagc aggcgaaagg tctgctcgat cagcgggtgg tcgtaggact ttgcccaggc
      361 cggaccgcag accgcaagca ggacgaacag gatcacccac ggcagcaaac gtcgcatcgt
      421 cagccacgtt ttcgggaaca tcaaagcgtc tcctgtgcga ccgaatcgtt acgttcgatg
      481 gggccgatgc ggcaggacac actcaacatg acggcgtagc aatccttcca tatgtgaaag
      541 gggagtgaca tatggctggg cgtacccgtc gagaattcct caagactgcc gtcgccgccg
      601 gtgtcggagc ggctacggca accaagttca agaccatcaa tatcgttacg gctcaggcgc
      661 cgatcgtctg gaagatgcag tccggctggc cgacgcgcga cttcttccac gagtcggcca
      721 tcgagacgtc caagaaattc agtgagatga gcggcggccg catccgcgtg gacctcctgc
      781 cggccggctc cgtcgtgggc gcgttcggta ttatcgacgc cgtgcacagc ggcttgctgg
      841 atgccggaca cgcggtcggg gtgtacgcgt actcgaagtc cccgacgacg agtctgttcg
      901 gtaccggtcc gtcgttcggg atggacgcca ccgacatgct gtcgtggttc tattacggcg
      961 gcggcttcga catgtatcag gacctgctgc agaaccagct gaagctgaat gtcaccgcat
     1021 tcatgatggg ccccatgccc acgcagcctc tgggctggtt ccgcaacccg attctcaaag
     1081 cagatcagtt gaagcgaacg aagttccgca cggttggtct ctccgcggag ctcttcgcag
     1141 agatgggagc gtccgtcgtg atcctgcccg gtggcgagat cgtcccggcg ttggagcgcg
     1201 gggtgatcga cgccgcggag ttcaacaacc cctcgtctga taaagcgctg gggttgcccg
     1261 acgtccgaaa ggtctacatg ctgcagagct accatcaacc ggtggaaacc ctcgaggtga
     1321 caatcaacaa gggcaagtgg gacgcgttgt cggctgaact gaaggccatc gtgcgcggcg
     1381 cgatccttgc ggaatccgcc gactaccact accgtgcgat ccaccgcaac agccaggatc
     1441 tcgaagagat ggagcggcgc ggtatccggg tctttgcgac gccgagggaa atccttctgg
     1501 cgcagctcaa cgcatgggac aagatcgttg ccaagcgctc gaaggaggac gcgaacttcg
     1561 cacgcatcgt cagcagccag cgcaccttcg cgcgtcgcgt cgtgcgctgg aagcagcgca
     1621 tctacgtcga caactcgatc gcgttcaacc ggtacttcag agcgtccaag tagcaaacgg
     1681 gccatctggc ctgaaggcgt cagggcgccc cgggacatac ttcccagggc gccctaatca
     1741 tgcacgggga cttccacatg cagaaatgga tcaagcggat tgatcgactg agcgaaatcg
     1801 tcggcacatc cgcggcttgg cttgtcgtga tcatgacgct ggccatgtcc accgaggtgc
     1861 tgtcccgccg actgctgggg cggcccacga cgtgggcctt tgacgtcagc tatatgagct
     1921 acggcgccta ctttttccac ggctcggcct atacattggc aaagaacgcg cacgtccgcg
     1981 gcgacatctt ttaccgcaac ttctcaccac ggacgcaggc gatcattgac ttctcgcttt
     2041 acctgctcgt cttctttccc gcaatgatcg cgatggtcgt catcggcgcg gattatttct
     2101 gggagtcgta cagaattcga gagacctcgc cgctcagtcc gtacgacacc cctgtctatc
     2161 cgctcaaagc ggtcattcca atcggagcat tccttctgtt agtccagggt gtcgcgcaag
     2221 tgatgcgaac gattatcgcc atccgaacca accgctggca tgatcaggag cgggaagcgg
     2281 aggtcgtcgc gtgagccagg aagcgctcgg catgataatg ccggggctgc tgaccgccgc
     2341 gatct
//
LOCUS       DBNGSB010000161         6812 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3334740, whole genome shotgun sequence.
ACCESSION   DBNGSB010000161 DBNGSB010000000
VERSION     DBNGSB010000161.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 6812)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6812)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6812
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3334740"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="AC159B_04655"
     CDS             <1..954
                     /locus_tag="AC159B_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3377069.1"
                     /translation="RRPGGTVQAVSDVSFTVDAKESVAIVGESGSGKSTLARCVLRMI
                     EPTSGRIVFDGTDVTELKGADLKRMRRRMQIIFQDPYSSLNPRMTVGDIIGEPLEIHN
                     LARGKEKLRRVQELLEVVGLSPYHVNRYPHEFSGGQRQRIGIARALAVNPKLIICDEP
                     VSALDVSIQAQVLNLLQELQKEFGLTYLFIAHDLSVVKHISDRIAVMYLGKIAEIAEA
                     DEMFENPQHPYTEALLSAVPIPDPGMRRERIILPGDVPSPVNPPQGCRFHTRCLYARE
                     SCRVNEQVLIDYWGNGHEVACEVLPFRHQRSKAAVVKQTAS"
     gene            1051..1353
                     /gene="gatC"
                     /locus_tag="AC159B_04660"
     CDS             1051..1353
                     /gene="gatC"
                     /locus_tag="AC159B_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HIR3377070.1"
                     /translation="MAITASIVDYVAQLARLELSTEERERLAIELAAILDHLAKLNEL
                     PTTGVEPTSHVIPMTNVFRDDSVEASLPRETVLEGAPERDGEFFKVPPIIETEPGP"
     gene            1374..2837
                     /gene="gatA"
                     /locus_tag="AC159B_04665"
     CDS             1374..2837
                     /gene="gatA"
                     /locus_tag="AC159B_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HIR3377071.1"
                     /translation="MLSRLAGREISSVDLVRACLDRIDAVDPRVHAFLSVDRSSALDA
                     ARRWDARRAHGDAVPPLAGLPIAIKDIICSQGLPTTCGSRILEGWRPPYDATVVIRLR
                     EAGAIVIGKTNLDEFAMGSSTENSAFGPTRNPWDLTRVPGGSSGGSAAAVSAGMVPLA
                     LGTDTGGSVRLPAAYTGIVGLKPTYGRVSRYGLVAFASSLDQVGPLARTVADAALLLQ
                     VIAGEDPLDSTSVNLAVPDYSASLTGGVKGLRIGIPREAVGAGLQREVKDAVLAAARV
                     LEQAGAAITEVSLATIEHAVPTYYILAPSEASANLARYDGVKYGLRVRTPDIHTMYTR
                     TRGEGFGSEVKRRIMLGTYALSAGYYEGFFLKAQQVRTLVRRDFTDAFRTVDLVLMPV
                     APTVAFKIGEKVLDPLQMYLTDIYTIPVNLAGLPGISVPCGSANGLPIGLQLVGKPFD
                     EATVLRAAHTYEQATEWHKRRPPIDGNAGTRERGNAS"
     gene            2837..4303
                     /gene="gatB"
                     /locus_tag="AC159B_04670"
     CDS             2837..4303
                     /gene="gatB"
                     /locus_tag="AC159B_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIR3377072.1"
                     /translation="MTTKAAGLGYEVVIGLEIHVQLLTASKMFCGCPTTFGAPPNTQT
                     CPVCLGLPGSLPVINRKAVELGLRTAVALGCRVHETSQFHRKNYYYPDLPKNYQITQY
                     EYKIHPPLGTKGSLELTAEASPRRIGIRRVHLEEDTGKLVHVEQHGQILHSLIDFNRS
                     GVPLMEVVTEPDLRSPEQARLFLGRLRSLLQAIGVSSGRMEEGSLRCDANVSLRRPGA
                     DLGIRTEVKNMNSMRSVERALAFEAQRQEQLLARGQPVAQETRHWDERRGVTFSSRTK
                     EEAQDYRYFPEPDLVPIAVGQQWIDEIHSSLPELPEARRQRYASALGLSAYDAGLVTS
                     SMPLAIFFEETVRHFPQAKTVANWLAGDVAAHLNAEGVEIEETALTPTHLAGLLALVD
                     AGAISIRTAKEILPDMLRTGASAEQLVADRGLAQISDTAAIGGVIDQIVAEHPGPASD
                     FRAGKVQALTFLVGQVMKKTRGRANPEMVNKLLREKLR"
     gene            4435..5760
                     /gene="rlmD"
                     /locus_tag="AC159B_04675"
     CDS             4435..5760
                     /gene="rlmD"
                     /locus_tag="AC159B_04675"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HIR3377073.1"
                     /translation="MNLDGAAVASSAMQQIAVPFALPGEEAVVEITRGGKEALGKIVT
                     IVRKAAEAASPRCRHFGLCGGCQWQHLPYDAQLRHKTTLVRQSLAPILRGLRVPVRDA
                     IGASPWEYRNRIQAAFDVRQHRVMAGYYTMREPAQSNGDEPSIFNVQECPIQHADNVR
                     LLTAARDVIAELAWPVYDRRSGRGLVRGIVGQVGFGSGEAMLVLSTTAEVPDRMAIVH
                     AVRRRILNLTSLLLSVQPLHTPELLGRISLLWGRPYIEDEIAGLGVRLYPSASVPPNP
                     RAMPLWLDAIAHGAEASTDAAIFDAACEEGFVPIALARRGARVVGVAPDRDAMHRAWE
                     NARLNDVDSVLFYTRAPSGVLAKLHDRGQRFTSLLATGRGVRALEPLFLAAKHSGVRR
                     VVCGGHSPARLALALRAAMDAGYRPIEIQPVDLLPQTSRIHCVATLARE"
     gene            5793..>6812
                     /locus_tag="AC159B_04680"
     CDS             5793..>6812
                     /locus_tag="AC159B_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012809748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR3377074.1"
                     /translation="MSVTIKHTFPGAKSKALLDEKRRYVSDAIGIHLPADIVSARGAV
                     LVDADGNTFIDLAGGVGCLNVGHSHPRVVRAIVEQAQRFTHTDFSVAPYESYVRLCRR
                     LCEIAPGGRPKKAALFNSGSEAVENAVKIARAVTGRPAVIAFQGAFHGRTYMSLSLTS
                     KVEPYKKNFGPFMPEVYRVPFPDLYRSPEPDATRWVMAHLDELFTSHVQADRVAAIVA
                     EPILGEGGFVVPPPDFFPRLRALADRHKILLIVDEIQTGIGRTGKMWAVEHSGIEPDL
                     MTVAKSIAAGLPISAVVGLREIMDALPEGAIGGTYVGNPVACAAGLAVLDVLEEENLV
                     ARAATL"
BASE COUNT         1274 a         2140 c         2171 g         1227 t
ORIGIN      
        1 cggcgccccg gcgggaccgt gcaggccgtg tccgacgtca gcttcaccgt cgacgccaag
       61 gagtcagtgg cgatcgtcgg cgagagcggg agcggcaagt cgactctcgc ccgctgcgtc
      121 ctgcggatga tcgagccgac cagcgggcgg atcgtgttcg acggcaccga cgtcactgag
      181 ctcaagggcg ccgacctcaa gcgcatgcgg cgccgaatgc agatcatctt ccaggacccg
      241 tactcgtcgc tcaacccgcg catgacggtt ggggacatca tcggggagcc tctggagatc
      301 cacaacctgg cccgcggcaa ggagaagctc cgccgcgttc aggagctcct cgaagtcgtc
      361 ggtctgtccc cttaccatgt caaccgctat ccgcacgagt tcagcggcgg ccagcgccag
      421 cgcatcggca tcgcgcgggc gctggccgta aaccctaaac tcatcatctg cgacgagccg
      481 gtgtccgcgc tcgacgtctc catccaggcc caggtgctga acctgctcca ggaacttcag
      541 aaggagttcg gcctgacata tctgttcatc gcgcacgacc tgtccgtcgt gaagcacatc
      601 tccgaccgca tcgccgtgat gtatctcgga aagatcgcgg agattgccga agcggacgag
      661 atgttcgaga atccccagca cccgtacacg gaggcgctgc tctcggcggt cccgatcccg
      721 gatccgggga tgcggcgcga gcggatcatc ttgcccggcg atgtccccag cccagtcaac
      781 cctccgcagg gctgccggtt ccacacgcga tgcctgtatg cgcgggaaag ctgccgggtc
      841 aacgagcagg tgctcatcga ctactggggc aacggccacg aggtggcgtg cgaggtcctc
      901 ccgtttcgac atcagcgcag caaagctgct gttgtcaagc aaaccgccag ctgacggatc
      961 acgtcgggaa tggggaggcc gggtgggatg cccggcctcc ttactttcga gaagtcagag
     1021 gcatgctagg atgaatcaag ggactcagtg atggcgataa ctgcctcgat tgttgattac
     1081 gttgcccagt tggcccgatt ggagctttcc accgaagagc gcgaacggct cgcgatcgag
     1141 ttggcggcga tcctggatca cctggcgaaa ctgaacgagc tcccgacgac gggcgttgaa
     1201 ccgacttcgc atgtgattcc catgaccaac gtgtttcggg atgattccgt ggaggcgtcg
     1261 ctgccgcggg aaaccgtcct ggagggggcg cccgagcgcg acggagagtt cttcaaagtt
     1321 cccccgatta ttgagacgga gcccgggccc tgattgaagc gtccgccaca acgatgctct
     1381 cgcggctggc tggccgcgag atctcatcag tggatttagt ccgcgcctgc cttgaccgta
     1441 ttgacgccgt ggatccgcgt gtgcatgcct tcctctccgt cgacagatca tcggccctcg
     1501 atgcggccag gcgctgggat gcgcgccggg cgcacggaga cgcggtgccc ccgctcgccg
     1561 ggttgccgat cgcgatcaaa gacatcatct gctcgcaggg tctgccgacc acatgcggct
     1621 cccggatcct ggagggatgg cgaccgccct acgatgcaac cgtcgtcatc cgattgcgcg
     1681 aggccggcgc catcgtcatc gggaagacga accttgacga attcgcgatg ggttcgtcca
     1741 ccgagaactc cgcgttcgga cctacccgca acccgtggga tctcacgcgc gtacccgggg
     1801 gcagtagcgg cggatcggcc gcggcagtgt ctgccggtat ggtccccctc gcgctcggca
     1861 ctgacacggg cggctcggtc aggcttccag cggcttacac cgggattgta ggtctgaagc
     1921 ccacctacgg ccgtgtttct cgctatggac tggtcgcctt tgcctcgtca ctggatcaag
     1981 tcggtccgct ggcgcggacc gtggccgacg cggcgctgtt gctacaggtc atcgccgggg
     2041 aggaccccct ggactccacc tctgtgaatc tggcggttcc cgactactcc gcttcactga
     2101 ccggaggtgt gaagggcctg cggatcggca ttccccgcga ggcggtcggc gcaggactcc
     2161 aacgagaagt gaaagacgcg gtgcttgcgg cggcgcgcgt gctagagcag gcgggggccg
     2221 caatcactga ggtgtcgttg gcgaccatcg aacacgccgt gccgacgtac tacattctcg
     2281 cgccctcaga agcgagcgcg aacctggccc ggtacgatgg cgtgaagtat ggattgcgtg
     2341 tgcgcacgcc ggacatccac acgatgtaca cccgcacacg cggcgaagga ttcggcagtg
     2401 aggtgaaacg gcggatcatg cttggtacct acgcgctctc cgccggatat tacgaagggt
     2461 ttttcctcaa ggcacagcaa gtgcgcacgc ttgtccgaag agatttcacc gatgcgttcc
     2521 gcacggttga cctcgtgctc atgcctgtgg cgccgaccgt agcgttcaag atcggcgaaa
     2581 aggttctcga tccgctgcag atgtacctga cagacattta tacgattccc gtgaacctgg
     2641 caggtcttcc gggaatctcg gtgccatgcg ggtcggcgaa cggcctcccg atcggtctgc
     2701 agttggtcgg caaaccgttc gatgaagcga ccgtgctgcg cgccgcccac acgtatgagc
     2761 aagcgacaga atggcataag cgtcgaccgc cgattgacgg gaacgcggga acgcgggaac
     2821 gcgggaacgc gagctgatga ccaccaaggc agcgggtctc gggtacgagg tagtgattgg
     2881 gctggaaatt catgtccagc tcctgacggc ttcaaagatg ttctgcggct gcccgacgac
     2941 gttcggcgcc ccgccgaaca cgcagacctg ccctgtctgc cttggactgc cgggatcgct
     3001 gccggtcatc aaccgcaaag cggttgagct cgggctccgc acggccgtgg cgctgggctg
     3061 ccgcgtccac gagaccagcc agtttcatag aaagaactac tattatcccg atctgccaaa
     3121 gaactatcag atcacgcagt acgagtacaa gattcatccc cccttgggta cgaaaggctc
     3181 gctcgagctt acggccgaag cttccccgcg gcggattgga atccgccgcg tacatctgga
     3241 agaagataca ggtaagctgg tgcacgtcga gcagcacggc cagatcctgc actcgctgat
     3301 cgacttcaac cggagcggcg tgccattgat ggaagtggtg acagagccgg acctgcgttc
     3361 accagaacag gcgcgactgt tcctcggccg cctgcggtca ctcctccaag ccatcggggt
     3421 gagcagcggc aggatggagg aggggagtct gcgatgcgat gccaatgtgt cgttgcgccg
     3481 cccgggtgcg gaccttggca ttcggaccga ggtcaagaac atgaactcga tgcggtccgt
     3541 tgagcgcgcg ctggcgtttg aagcccagcg tcaggagcaa ctcctcgctc gcggtcagcc
     3601 tgtcgcgcag gaaacacgcc attgggatga gcgccgcggg gtgacgttct cttcacggac
     3661 aaaggaagaa gcgcaggact accggtattt tcccgagccc gacttggtgc cgatcgccgt
     3721 gggtcaacag tggattgacg aaatccactc gtcgctcccg gagctccctg aagcgaggcg
     3781 ccagcggtac gcgtccgcac ttggattgtc ggcgtatgat gcgggcctcg tgacgtcctc
     3841 gatgcccctg gcgatctttt ttgaggaaac cgtgcgacac tttccccagg ccaagaccgt
     3901 ggccaactgg ctggccggag acgtggcggc ccatttgaac gccgaaggcg tagagatcga
     3961 ggaaaccgca ctcaccccga ctcatctcgc cgggctgctg gctttggttg atgccggcgc
     4021 gatcagcatt cgcaccgcca aggagattct gcccgacatg ttgcggaccg gtgcgagtgc
     4081 tgagcaactg gtcgccgacc gcgggctggc tcagatcagc gacaccgctg ctatcggcgg
     4141 cgtcatcgat caaatcgtcg ccgagcatcc tggcccggca tcggactttc gtgcgggaaa
     4201 ggtgcaggcg ctgacgttcc tcgtcggaca agtaatgaag aaaacacgcg gacgcgcgaa
     4261 cccggagatg gtgaataagc tgttgagaga gaagctcaga tgagccgcaa gcgcgaaaac
     4321 gcgcaaacgc gcaagcgcga accaacgcag ggtcgtcgtt atgggccccg gaccccggac
     4381 cccgagcctc gggtcttgag ggtcggccag aaagctacct tgaagttttc ctcgatgaac
     4441 ctcgacggcg ctgcagtggc atcctcagcc atgcagcaga tcgcagtccc ctttgcgctt
     4501 ccgggcgaag aagccgtggt cgagatcacg cgcggcggca aagaagcgct gggcaagatc
     4561 gtcacaatag tccggaaggc cgcagaagcc gcctcgcctc gctgcaggca cttcggcctc
     4621 tgcggaggat gccagtggca gcacctcccc tacgacgcgc aattgcgaca caaaaccact
     4681 ttggtccggc agtcacttgc gccaatcctc cgcggtctac gtgttcctgt cagggacgcc
     4741 atcggtgctt caccgtggga ataccgcaac cgcatccagg cggcgtttga cgtcaggcag
     4801 cacagggtga tggccggcta ctatacgatg cgcgaaccgg cacaatcgaa tggagacgag
     4861 ccctctatct tcaatgttca agaatgtccg atccagcatg ccgacaacgt gcggctgctg
     4921 actgccgcga gggatgtgat cgccgaactg gcctggccgg tgtatgaccg ccgctccggc
     4981 cgtggcctgg tccggggaat cgtcggacag gtcggttttg gcagcggcga agccatgctc
     5041 gtcctctcga ccaccgccga ggtgcctgat cggatggcga tcgtgcacgc ggtgcgccgg
     5101 cgcattctca acctgaccag cctgctcctc tccgtgcagc cgctgcacac tccagaactc
     5161 cttggtcgca tcagtctcct ctggggacgc ccatatatcg aagatgaaat tgcgggattg
     5221 ggagtccggc tctacccttc cgcatcggtt ccgcccaatc cacgtgccat gccgctgtgg
     5281 ctcgatgcaa tcgcgcacgg tgccgaggcg tcgaccgatg ccgcgatctt tgatgcagcg
     5341 tgtgaagaag gctttgtacc gatcgcgttg gcgcggcgcg gggcacgcgt cgtcggcgtc
     5401 gctccggatc gcgatgccat gcaccgggcc tgggaaaatg cacggctcaa cgatgtggac
     5461 agcgtgctgt tctataccag agcgccgtcg ggagtcctag ccaagcttca tgaccgcggg
     5521 cagcgcttca cgagtctgct cgcaaccggc cggggagtgc gtgcgctcga gccgctcttt
     5581 cttgcggcaa agcacagcgg ggttcgacgc gtagtctgcg gcgggcactc gcctgcccgg
     5641 ctggccctgg cgctgagagc agcaatggat gccggatacc gccccatcga gatccaacca
     5701 gtagacctgc tgcctcaaac gagccgcatt cactgcgtgg cgactctggc ccgagaataa
     5761 tgggcagaat gaatcggagg ccagacactc ccatgtccgt gacgatcaag cacacctttc
     5821 ccggcgcaaa gtccaaggcg cttctcgacg aaaagaggcg ctacgtttca gacgccatcg
     5881 gcattcacct gccggcggat atcgtcagtg cacgcggcgc agtgctcgtc gacgcggacg
     5941 gcaacacctt catcgatctc gcgggtggag tcgggtgcct caacgtaggg cactcacacc
     6001 cgcgcgtcgt gcgcgcaatc gtcgagcagg cacaacgatt cacgcacacc gacttcagcg
     6061 tcgcgcccta cgagtcatat gtccggctct gccgcagact gtgtgagatc gcgccgggag
     6121 gaagaccgaa gaaggcggcg ctgtttaatt ccggcagcga agccgtggag aacgccgtca
     6181 agatcgctcg tgcggtcacg ggccggccgg cggtgatcgc gtttcagggc gcgttccatg
     6241 gccgcacgta tatgagcctc tcgctgacca gcaaggtcga gccgtacaag aaaaacttcg
     6301 ggcccttcat gcctgaggtc tatcgcgttc cctttcccga cctctaccgc agcccggagc
     6361 ccgacgcgac ccgttgggtg atggcgcatc tcgatgaact gtttacatcc cacgtgcagg
     6421 ccgaccgcgt ggccgcgatc gtcgccgagc cgatcttggg tgaaggcggg tttgtcgtac
     6481 ctccgccgga tttcttcccg cggcttcgcg cgctggccga ccggcataag atcctgctga
     6541 ttgtggatga gatccaaacc ggcatcggcc gcaccgggaa gatgtgggcg gtcgagcatt
     6601 ccggcatcga gcccgacttg atgaccgtgg ccaaatctat cgcggcgggg ctgcccatct
     6661 ctgcagtggt ggggctgcgc gagattatgg acgcgctccc agagggcgcc atcgggggta
     6721 cgtacgtcgg gaatcccgtg gcctgcgcgg ccgggttggc agtgctggat gtcctagaag
     6781 aagagaatct ggtcgcccgc gcggccactc tg
//
LOCUS       DBNGSB010000162         4223 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2720609, whole genome shotgun sequence.
ACCESSION   DBNGSB010000162 DBNGSB010000000
VERSION     DBNGSB010000162.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4223)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2720609"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="AC159B_04685"
     CDS             <1..936
                     /locus_tag="AC159B_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HIR3377075.1"
                     /translation="VDVGGILDIFYAVNEHASHTGASDAGYAVEVVSPVETVCAWPGL
                     RIVVNRSYRAVRGPVDTLIVTGVDRPDDARRDPELVRWLARMAPRTRRVVGLCTGSFV
                     LAEAGLLEGRSATTHWAFCGELARRFPGVRVQPNPIYVRDGSIYTSAGATAGIDLVLA
                     LVEEDFGRRVALAVARWMVIFLKRPGGQAQFSVQLSTQMADRQPLRDMQAWIFDHPGA
                     DLSVEALARRVNMSPRNFFRVFVREVGMTPGCFVERVRVEAARRLLEETSRGVPDVAT
                     TCGFGSQETMRVAFRRTLDVSPKGYRSRFRTTAVS"
     gene            960..2315
                     /locus_tag="AC159B_04690"
     CDS             960..2315
                     /locus_tag="AC159B_04690"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR3377076.1"
                     /translation="MIKQCALDDFRSTLRGDILLPQDADYDATRRIFNAMIDRRPALI
                     ARCASAADVVACIGFKRAEALEISVRGGGHNVSGKAVCDGGLMIDLSRMKGCRVDPLR
                     RRARAEAGLTLADLDRDCQAFGLATPTGIVSPTGIAGLTLGGGIGWLGGKHGLSCDNL
                     ESVDIVTADGRLLVAAPTEHPDLFWAVRGGGANLGVVTSFEYRLHEVGPVVGGGIAWP
                     LARAKHVLPFYDEFARASPDELSLNAGFFTVEDGTKLVGVKVAWIGPLDVGERLLKPL
                     RSLDSPVADGIAPMGFVDLQRGGDGAFPRGRRHYWKASFLRRLEQGAIDVLVEFTATC
                     PSPYTQVGLQQMHGQAARVSPTETAFAHRHDQWDCLILTQWDDSADDERNIRWTRELH
                     AALRPWVEDAVYVNNLGDDEADRVRSAYGANFDRLVAVKAKYDPDNFFRGNQNVAEHR
                     I"
     gene            2400..3527
                     /locus_tag="AC159B_04695"
     CDS             2400..3527
                     /locus_tag="AC159B_04695"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR3377077.1"
                     /translation="MAKVERVLIVGGGLAGLALMIALRQRGRSAAIVERGSDRTAPGT
                     GLYLIGSATRALQALGVAADAERNGCVIRTQCLFNHRGTRLASIDAGAFWEPCGPCFG
                     IARSDLHKLLTQQAAGSHIRLGVTVQSLRQHEDHVRVRLSDGSCETYDLVVGADGIRS
                     SIRQLEYGDLGVEFRGQVGWRFIVRRPPGLEGWTVFLGPQSAFLMLPIGNELAYCYAD
                     KASARSIHDPAQRSIERLRENFAAFASPIPEVLARLEPADPIHFSAIEEVAHPCYGQG
                     RVVLIGDAAHAMSPNMACGAAMAFEDALVLAEIVSRAGAASEVVPKFVRRRSARVGWV
                     RQQTDRRDHLRSLPPTMRDVFVRLLSGRLYRANYRPLLALP"
BASE COUNT          700 a         1357 c         1385 g          781 t
ORIGIN      
        1 gttgacgtgg gtggcatcct cgacatcttc tatgccgtca acgagcatgc cagccatacc
       61 ggtgcatccg atgcgggcta cgccgtcgag gtggtgtcgc cggtcgagac tgtctgtgcc
      121 tggccggggc tgcggatcgt cgtcaatcgg tcgtaccgcg ccgtccgtgg tcccgtcgac
      181 actctgatcg tcaccggggt cgaccgtccc gacgacgcga ggcgcgaccc ggagctcgtg
      241 cgctggctgg cgcgcatggc accgcggacc cggcgagtgg tcgggctctg tacgggatcg
      301 ttcgtcctgg ctgaggcggg gctcctggag ggccgcagtg cgacgacgca ctgggccttt
      361 tgcggcgagc tggcgcggcg ctttcctggc gtcagagtcc agccgaatcc catctacgtt
      421 cgggacggca gtatctacac atcagccggc gccacggcgg gcatagatct cgtgctggcg
      481 ctagtggagg aggactttgg ccgtcgcgtg gccctggcag tggcccggtg gatggtcatc
      541 ttcctcaagc ggccgggagg ccaggcgcag ttcagcgtcc agctctccac gcagatggct
      601 gatcgccagc cgctccgcga catgcaggcc tggattttcg accatcccgg cgccgacctc
      661 tccgtggaag cgctcgcgcg tcgggtgaac atgagcccgc gcaacttctt ccgcgtgttc
      721 gtgcgcgagg tgggcatgac gccggggtgt ttcgtggagc gcgtgcgcgt ggaggcggcc
      781 cggcggctcc ttgaggagac atcgcgcggc gttccggacg tcgcgaccac gtgcgggttc
      841 gggagccagg aaacgatgcg cgtggccttt cggcgaacac tggacgtgag cccgaagggc
      901 taccggagcc gcttccgcac gaccgccgtg tcgtaggaga ttctcaaagg aaaacaaaga
      961 tgatcaagca atgcgcgctc gacgacttcc gctccacgct tcgtggcgat atcctgcttc
     1021 cccaggacgc cgactacgac gctacccgtc ggatcttcaa cgccatgatc gaccggcggc
     1081 cagccctgat cgcccgctgt gcgagcgcgg ccgatgtggt cgcctgcatc gggttcaagc
     1141 gggcggaggc gctcgagatc tccgtgcggg gcggtgggca caacgtgtcc ggcaaagcgg
     1201 tgtgcgacgg cgggctgatg atcgatctct cgcggatgaa gggctgccgc gtcgatccct
     1261 tgaggcggag ggcccgcgct gaggcgggct tgaccctcgc agacctcgac cgcgactgtc
     1321 aggctttcgg cctagccacg cccaccggca ttgtctcccc aaccgggatc gccggtctga
     1381 ccctgggcgg cggcatcggg tggctcggag gcaagcacgg tctgtcgtgc gacaatctcg
     1441 aatccgtcga catcgttacc gccgacggcc gcctgctggt cgccgccccc accgaacatc
     1501 ccgacctctt ctgggcggtg cgcggcgggg gtgccaacct cggcgtggtc acctccttcg
     1561 agtaccgcct gcacgaggtt ggacccgtgg tgggcggcgg aatagcatgg ccgcttgccc
     1621 gcgccaagca cgtgctgccc ttctacgacg agttcgcgcg cgccagcccg gacgagctga
     1681 gcctcaacgc cgggtttttc accgtggaag acggtaccaa gctggtcggc gtcaaggtcg
     1741 cgtggatcgg tccgctagac gtcggtgagc ggctgctcaa accgctgcgg tcacttgaca
     1801 gtccggtcgc cgacggcatc gcccccatgg gcttcgtgga tctgcagcgg ggcggtgatg
     1861 gcgcgtttcc acgcggtcgc cggcattact ggaaggcgag cttcctccgc cgtctcgagc
     1921 agggcgcgat cgacgtgctg gtcgagttta cggccacctg cccctcgccg tacacacagg
     1981 tcggtctcca gcagatgcac gggcaggcgg cgcgggtgtc cccgacagag acggcgttcg
     2041 cgcatcgtca cgaccagtgg gactgtctca ttctaacgca gtgggacgat tccgccgacg
     2101 acgagcgcaa cattcgctgg acgcgcgagc tgcacgccgc actgcggccg tgggtggagg
     2161 acgcggtgta cgtgaacaac ctcggtgacg acgaggccga ccgcgttcgc tccgcctacg
     2221 gcgcgaactt cgaccggctc gtggccgtca aggcgaagta cgacccggac aacttcttca
     2281 gaggcaacca gaacgtcgcg gaacatcgca tctgatgcac gagaagcacg cattggccta
     2341 caaggcagca gtccagtcct tcctggcaac gcaccgtcag gcgacctgag gcacgactca
     2401 tggccaaggt cgagcgcgtg ctgattgtcg gtggcggact ggcaggcttg gcgctgatga
     2461 tcgccctgcg tcagcggggg cggtcggccg ccatcgtcga gcgggggagc gaccggacag
     2521 cgcccggcac cggtctgtac ctcatcggta gcgcgacgcg cgccctgcag gcgctgggag
     2581 tcgccgccga cgcggaacgc aacggctgcg tcatccgcac ccaatgcttg ttcaaccatc
     2641 gaggcacgcg cctcgcctcg atcgacgccg gagcattctg ggagccatgc ggcccatgtt
     2701 tcgggatcgc gcgcagcgat ctgcacaagc tgctcacgca acaggcggca ggctcgcaca
     2761 ttcgactcgg cgtgacggtg caatcgcttc ggcagcatga ggatcacgtc cgggttcggc
     2821 tgagcgacgg ttcctgcgaa acctacgacc tcgtcgtggg cgcggacggc atccggtcgt
     2881 ccattcggca attggagtac ggagacttgg gcgtcgagtt tcgcggccag gtgggatggc
     2941 gtttcattgt tcgtcggccg ccgggtctcg aaggctggac ggtgtttctc ggcccgcagt
     3001 ccgcattctt gatgctgcca atcggcaatg aactggcata ctgctacgcg gacaaggcca
     3061 gtgcccggtc catccatgat cctgcgcaaa ggagcatcga aagactccgt gaaaatttcg
     3121 ccgccttcgc cagtccgatt cccgaggttc ttgcccggct cgagccagcc gacccgatcc
     3181 atttctcggc catcgaggag gtcgcgcacc catgctatgg ccaaggacgc gtcgtgctga
     3241 tcggcgatgc agcccatgcg atgtcgccca acatggcgtg cggcgcggcg atggccttcg
     3301 aggatgcact ggtcctggct gagattgttt cgcgcgcggg cgccgcatcg gaagtcgtcc
     3361 ccaagttcgt ccgccggcga tcggcgcgtg tgggctgggt gcgccaacag accgatcgcc
     3421 gagaccatct gcgcagtctt cctccgacga tgcgcgacgt gttcgtccgc ctgctgtcgg
     3481 gccggcttta cagggccaac tatcggccgc tgctggctct tccataaccc atgagatgcc
     3541 tcctcccgcg aaggaagcgc aagacctcgc ccgagcgccg tgcagcccag ctacttgatt
     3601 ggtccgaagt ctttgcacct ggacacttcg ccccctgatg gtttttcttc tgatgcaggt
     3661 cactatcacc cagcggcggc gcatctacgc ctgccgaaca cacggaatgg aatcgtggag
     3721 attgcggcaa cgatcatgcg aaaccccaaa gaaatcctta tcgagcctgg ctaagtcagc
     3781 gagattccgt ggtcggtcgt cccgcaaata ttcgtggtta gggcactatg ctattcagat
     3841 ggcaagaatc gactggatta gagtgctata gcgtagagcc catcatcgtc gggcgtggcc
     3901 agcccgaaga gattcgaact cctgacctac ggattcgtag gctggtgccc gtgtcagagg
     3961 ttcagaccgt cttagtctgc gacttctgct ccgggtcaat cgcgtcgttc tgaccgtgct
     4021 ccgttcactt ccggtcttcc ccgattagcg aacattctcg gggtcagtcg gcatgtctca
     4081 aacgtgccac aagccgacat aggccgttga gggccggatg gcctttagca accggactta
     4141 tcttgcagac aatgtgggac gttcgcgagc gcgatcaaat tttcgccgat cttcgatcgc
     4201 gatccaagca caaagccatg cca
//
LOCUS       DBNGSB010000163         4528 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_1037207, whole genome shotgun sequence.
ACCESSION   DBNGSB010000163 DBNGSB010000000
VERSION     DBNGSB010000163.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4528)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4528
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_1037207"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..2151
                     /gene="pheT"
                     /locus_tag="AC159B_04700"
     CDS             85..2151
                     /gene="pheT"
                     /locus_tag="AC159B_04700"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIR3377078.1"
                     /translation="MKVLVSWLREYVDIPFSLDELEERLSMLGLGVEGVERIGRDDAV
                     FDLEIAANRGDLMSHLGVARELAAATKSTVRLPSAEVARDRTAVSEFVRVEVREPDLC
                     PRFTAAMLVDVRVAPSPEWLARHLEACDVRSINNIVDVTNYVMLEMGQPMHAFDYDRL
                     HGGHLVIRPARSKEPLTTLDGVQRTLDPQTLLVADADQGAGIAGIIGGAPTEITTSTR
                     RVLLEAAVWHPPMIRRTSKRLGVRTESSARFERGVDQAGVPSASTRALALMQQVAGGR
                     ALDGLIDVQARQIPTRQIAFNWSTVHRVLGTDVPIKEGVATLKSLGFQVEAKDSTVRV
                     VVPSYRRDVEREEDLVEDIARHHGYDRIPEEMPVEVTAQGSWAPSIDAERAIRDTLIR
                     AGLTEVLTVPLTIAAALDTLRLPDRHPFRDAVPIRNPMVEDHVLLRASLLPGLLHVAR
                     TNASHRSTDIQVFEVGRTFHPNREGAREERSLGVLMTGVTLRGAWNLPPESLRVTYYT
                     LKGVMESLLGELRVAKAEFRATHAPWLHPGRAASLVLEGKTIGTLGELHPDVGRDLDL
                     PEVTCIAEVDLQALLAHAVLQPRFVPPPRYPSVRRDISFVVDAGVPVAEVEAAIRRAG
                     GGLLERAELFDIYTGTPVPEGRRNLAYALTFRSPDRTLTAEEISETMAAIARALEKGL
                     RAKLRE"
     gene            2166..2678
                     /locus_tag="AC159B_04705"
     CDS             2166..2678
                     /locus_tag="AC159B_04705"
                     /inference="COORDINATES: protein motif:HMM:NF014704.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HIR3377079.1"
                     /translation="MTWLDWTIASVVLFAALQGFRRGALVTLVSAVGVIVAYLAASVW
                     YRPLTDIVQRYLRFSGSWAATISFVALLLAIYVVIGVAVTAVAGAAKLSPASRFVGTV
                     LGMLKGALLAVALLIAAIGSPIGAPINRDAERSALAAYAVQAHKIGATTLDGVLPDSI
                     RPFGLSDEKF"
     gene            2682..4397
                     /gene="polX"
                     /locus_tag="AC159B_04710"
     CDS             2682..4397
                     /gene="polX"
                     /locus_tag="AC159B_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HIR3377080.1"
                     /translation="MKNRAIAAMFNDIADMLEIKGETPYRITAYRRAARSLEGLTEDI
                     AVVAERGTLEEIPGVGKSTAEKILEFLKTGTCTYYEELRKQLPAGLTTLMTVSEVGPK
                     TAMLLHEKLGIDSIEDLEQAARKGLVRELPRMGAKTEENILRAIALLRRSKERLPIGA
                     VLPLSREIVDALRGLTAVKQISVAGSLRRMRETIGDIDILVTSSKPEKVMDVFTTLPR
                     VRQVLAKGPTRSSVILDTGIQADVRVVEPEAFGAALQYFTGSKEHNVKVREKAVRRGL
                     KVNEYGVFRVKDDRRTAGTTEEELYQAVGLPWIPPEIREDQGEIELADRGTLPRLLDL
                     KDVRGDLQMHTRWSDGSDSIEAMAKAARELGYEYIAITDHSQSLKFVGGVPIDELREH
                     IKVARRMSEKIGVTILMGTECDILPDGRLDYPDEVLKELDVVVASVHSRLRMAEDEMT
                     ARIVKALDNPHVDILGHPTGRLLGSREAYAVNIERLIEAAARTKTALEINASPQRLDL
                     SDKHARLARERGVSLAISTDAHTRYQLGYMEYGVAIARRAWLEPGHVINTLPLTALTS
                     WLHDR"
     gene            4387..>4528
                     /locus_tag="AC159B_04715"
     CDS             4387..>4528
                     /locus_tag="AC159B_04715"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014316.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HIR3377081.1"
                     /translation="MTGREPLPRTFFARSPVAVARDLLGCYLVHETQEERLVGRIVEA
                     EAY"
BASE COUNT          860 a         1359 c         1486 g          823 t
ORIGIN      
        1 gggataacga tctgcgggtg cttgagcaat tctagccggt gcgggcggcg cgctgcgcgc
       61 agcgcgaagc ccagagggcg tgcgatgaaa gtcctggttt catggctgcg agagtacgtg
      121 gacatcccct tcagtctcga tgagctggaa gagcgtctct cgatgctcgg cctcggcgtg
      181 gaaggcgttg agcgcatcgg tagggatgac gccgtgttcg atctggagat cgcagccaat
      241 cgtggggatc tgatgagtca tctcggcgtc gcgcgggagc tcgcggccgc gaccaaatct
      301 acggtccggc tcccctcggc agaggtggcg cgtgaccgca cggcggtttc ggagtttgtt
      361 cgggtcgagg tccgcgaacc cgacctgtgc ccgcgattca cggccgccat gctcgtagac
      421 gtgcgcgtcg cgccttcgcc cgaatggctc gcgcgccatc ttgaagcctg cgatgttcgg
      481 agcatcaaca acatcgtgga tgtgaccaac tatgtgatgc tcgaaatggg gcagccgatg
      541 catgccttcg actatgaccg gctccacggt ggacatctcg tcatccggcc cgcacgatcc
      601 aaagagccgc tgaccacgct ggacggggtc cagcggaccc ttgacccgca gacgctgttg
      661 gtcgcggatg cagatcaagg ggcgggaatc gccggcatca tcggcggagc cccgaccgaa
      721 atcacgacct cgacgcgccg tgtgttgctg gaggctgcgg tgtggcaccc gcccatgatt
      781 cgccgcacca gtaaacggct tggggttcgg acggagagca gcgcgcgatt cgagcggggg
      841 gtagaccagg ccggcgtgcc ctcggcgtcg acgcgagccc ttgccctgat gcaacaggtc
      901 gccgggggcc gcgcgctgga cggactcatc gatgtccagg cccgtcagat tcccacaaga
      961 cagatcgcgt tcaactggtc gaccgtgcac cgtgtgctgg ggacggacgt gcccatcaaa
     1021 gaaggcgttg ccacgctgaa gagccttggt ttccaggtcg aggccaaaga ctccacggtg
     1081 cgcgtcgtgg tcccatcgta ccgtcgagac gtggagcgcg aggaagatct cgtcgaggat
     1141 atagcccggc accacggtta cgatcgcatc ccagaagaaa tgccggtcga ggtgacggcg
     1201 cagggttcat gggcgccatc catcgatgcg gagcgtgcca tccgcgatac tcttatacgg
     1261 gcaggcctga ccgaggtgct gacggtgccg ctcacgatcg ccgcggccct cgacacgctg
     1321 cgcctgcccg acagacatcc gttccgagac gcggtaccga tccgcaatcc catggtggaa
     1381 gaccacgtgc tcctgcgggc atcgttgctg cccggcctcc tgcatgtcgc gcgtaccaac
     1441 gcgagccatc ggtcgaccga catccaggtc tttgaggttg ggcggacctt tcatccgaac
     1501 agggaggggg cgcgagagga gcggtcgctg ggagtgctga tgacgggcgt gacgctgcgc
     1561 ggcgcatgga atctgccgcc cgagtctttg agggtgacgt actacacctt aaaaggcgtc
     1621 atggaatccc tgctcggcga gctccgagta gcgaaagctg agttccgggc cacgcatgcc
     1681 ccctggctgc acccaggtcg tgcggcttcc ctcgtgctgg aggggaagac gattggaacg
     1741 ctgggcgaac tccatccgga cgtgggccgg gatcttgatc tgcccgaggt cacctgcatt
     1801 gccgaagttg atttgcaggc attgctggcc cacgctgtac tgcagccgcg gtttgtgccc
     1861 ccaccgcggt atccatcagt gcgccgtgac atctcatttg tcgtcgatgc gggcgtccca
     1921 gtcgcggagg tggaggcggc gatccgtaga gccgggggcg ggctcctgga acgcgcggag
     1981 ctgttcgata tttacaccgg cacgccggta cccgagggac gccggaacct cgcctacgcg
     2041 ctcaccttcc gctcgcccga ccggaccctc acggccgaag aaatcagcga gacgatggct
     2101 gcaatcgcac gagcgctcga aaaggggctg agggcgaagc taagagagta aaatagaggt
     2161 atccgatgac gtggctggat tggacgatcg cttctgtagt gctgttcgcg gcgctccagg
     2221 gattccgacg tggagcgctc gtcacgcttg tgagcgcggt gggcgtcatc gtggcctatc
     2281 tggccgcttc ggtgtggtac cgtccactca cggacatagt ccagcggtat ctccgattct
     2341 ccggctcctg ggcagcaacc atttcgttcg tagcgctctt gctcgcgatc tacgtggtga
     2401 tcggcgttgc tgtcaccgcg gtagcagggg cagccaagct ctcacccgca tcgcggttcg
     2461 tggggacagt actggggatg ctcaaaggtg cgctcctcgc agtggcgctt ctgatcgcag
     2521 ccattggctc cccgatcggt gcgcccatca accgtgatgc cgagcggtcc gcgttggcgg
     2581 cctatgccgt tcaggcgcac aagatcggcg ccacgacgct cgatggtgtt ctacccgact
     2641 ccatccggcc gttcggatta tcagacgaga agttctgaca gttgaagaac agggcgatcg
     2701 cggcgatgtt caacgacatc gccgacatgc tcgagatcaa aggcgaaacc ccctaccgta
     2761 ttacagccta ccggcgtgcc gcgcgatcac tcgaagggct gactgaggac atcgcggtcg
     2821 tcgccgagcg cggcacgctg gaagaaatcc cgggtgttgg gaaaagcacg gcggagaaga
     2881 ttctggagtt cctcaagacc ggaacctgca cctattatga ggagctgcgc aagcagttgc
     2941 ctgccgggct caccaccctc atgacggtgt ctgaagtggg gccgaagacc gcgatgctgt
     3001 tgcacgaaaa actggggatc gacagcatcg aagacctgga gcaagcagcg aggaaggggt
     3061 tggtgcgcga gcttccccgg atgggggcaa agacggagga gaacatcctc agggccatcg
     3121 cgctccttcg gcgcagcaag gaacggcttc ccatcggtgc ggtcctgcct ctttcgcgcg
     3181 agatcgtcga tgcgctccgc ggtctcacag ccgtcaaaca gatcagtgtt gcggggagcc
     3241 tgcgccgcat gcgtgaaacg atcggcgaca ttgatattct ggtcacaagc agcaaaccgg
     3301 agaaagtcat ggatgtcttc acgaccctgc cccgggtcag acaggtcctg gcgaaggggc
     3361 cgacgcgatc cagtgtgatt ctcgatactg ggatacaggc agatgtacgc gtcgtggagc
     3421 cggaggcctt cggcgcggcc cttcagtatt ttaccggtag caaagagcac aatgtaaaag
     3481 tccgggaaaa ggccgtccgg cgcgggctca aggtcaatga gtacggtgta ttccgtgtca
     3541 aagacgaccg gcggacggcg ggcacgaccg aggaagagct gtatcaggcc gtgggcctcc
     3601 cgtggattcc gccggagata cgagaggatc agggtgaaat cgagctcgcc gaccgcggga
     3661 cgttgccgcg tctccttgat ctaaaagacg tccgcggcga cctgcagatg catacgcgct
     3721 ggagcgatgg gtcggattca atcgaagcaa tggcgaaggc cgcgcgagag ctcggttacg
     3781 agtacatcgc tataaccgat cactcgcaat cgctcaagtt tgtcggcggc gttcccatcg
     3841 acgagttgag ggagcacatc aaggtcgccc ggcggatgtc tgaaaagatc ggtgtcacga
     3901 tcctgatggg gacggagtgt gacatcctcc cggatggccg gctggactat ccggacgagg
     3961 tgctcaagga actcgacgtg gtcgtggcct ctgtgcattc tcggctccgt atggctgagg
     4021 atgagatgac cgcgcggatc gttaaagcgc tcgacaatcc acatgtcgac atcctgggcc
     4081 atcccaccgg acggctgttg ggatcacggg aagcctacgc cgtgaatatc gagcggctca
     4141 ttgaggcggc tgcgcgcacc aagacggccc tcgagatcaa cgcttcgccc cagcgcctcg
     4201 acctgagcga caagcacgcc cgcctggccc gcgagcgcgg ggtttcgctg gccatcagca
     4261 ccgacgccca tacccgatat cagttgggtt acatggaata cggcgtcgcg atcgctcggc
     4321 gcgcctggct ggagcccggg cacgtgatca acaccttgcc cctcacggcg ctcacctcgt
     4381 ggctgcatga caggtagaga acctcttccg cgcacattct tcgcgcgcag tcccgtcgca
     4441 gtcgcaagag atctcctggg ctgctatctc gtccatgaaa ctcaagaaga gcgtctggtt
     4501 ggacgtatcg ttgaagccga agcctacc
//
LOCUS       DBNGSB010000164         6059 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2415876, whole genome shotgun sequence.
ACCESSION   DBNGSB010000164 DBNGSB010000000
VERSION     DBNGSB010000164.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 6059)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6059)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6059
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2415876"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="AC159B_04720"
     CDS             <1..506
                     /locus_tag="AC159B_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid kinase family protein"
                     /protein_id="HIR3377082.1"
                     /translation="MLGTAINALALQAVMEGRGLVTRVQTAIEMHQIAEPFIRRRAMR
                     HLEKGRVVIFAGGTGSPYFTTDTAAALRAIEIEAGALLMAKKGVDGVYDKDPNVHPDA
                     VIYRRLGYLDVLSQDLKVMDATAVALCKDNNMDIIVFDVAQPGNVKRAVLGEEVGTLV
                     TSSGRAR"
     gene            503..1060
                     /gene="frr"
                     /locus_tag="AC159B_04725"
     CDS             503..1060
                     /gene="frr"
                     /locus_tag="AC159B_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HIR3377083.1"
                     /translation="MIADVISDTRSRMAKAIDATKHEFSSLRTGRASPALLEQIRVDY
                     YGTPTPISQMATITVPEPRLLVVQPWDRKMVKDIERGILKSELGLVPSSDGTHVRVPI
                     PSLTEERRKELVKIAHRHAEEGRVAIRNVRREAKEMIEQLEEDGDISEDDAKRGLDEL
                     QKLTDKYIADVEALLASKDKEIMEV"
     gene            1061..1294
                     /locus_tag="AC159B_04730"
     CDS             1061..1294
                     /locus_tag="AC159B_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HIR3377084.1"
                     /translation="MIDVIGLELEEARRRLEAAGMVVAVSETRSPIPTVRLTGPMRVV
                     RQRVTSEGVELVVTAERYDPASLRVNAPRPRDR"
     gene            1284..2048
                     /locus_tag="AC159B_04735"
     CDS             1284..2048
                     /locus_tag="AC159B_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HIR3377085.1"
                     /translation="MTDSRSAEPLSATQAILDPTRIPTHIAIIMDGNGRWASSRGLNR
                     AAGHRAGLDAVRRTLEASRELGVAVLTLYAFSTENWRRPAEEVEALMQLLGEAVSDEA
                     DELMRKGIQLRVSGDLSSIDPGLRREVERVVALTKDNGDVILNVAYNYGGRAEILSAA
                     RRLAGDAAAGRLPADSIDEAALGRYLYTAGLPDPDLLIRTGGEQRISNFLLWQIAYTE
                     LYFCDVYWPDFDKVHLSAAIQTYQQRLRRFGGIESS"
     gene            2056..2880
                     /locus_tag="AC159B_04740"
     CDS             2056..2880
                     /locus_tag="AC159B_04740"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIR3377086.1"
                     /translation="MDQVTSTFARAKLLPRTMTALIGGGAFLTAAWAGGVWWVAIMAI
                     LALLAGIEYAGLHPQVAARNAVPAGAVLAVLLAGAGIWMDQLLLMLGAFVFFGGVVPW
                     IARGMDPHRWAQPAMLILVQGVAYVGVPLGVLARWRLQGTFDGVLWMLVIVWAGDIAA
                     YFVGLLVGRHKMAPKVSPGKSWEGAAAGLAAGAFAGLLAGPVFGLGPLGAIALGAITS
                     ITAQSGDLFESALKRRAGVKDSGGLLPGHGGVLDRFDAVFFAAPVAYGLLKLWGRL"
     gene            2888..4039
                     /locus_tag="AC159B_04745"
     CDS             2888..4039
                     /locus_tag="AC159B_04745"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HIR3377087.1"
                     /translation="MKRIIILGSTGSIGRQALEIVDAAPGDFQVAGLGARRDAETLTA
                     QARKYKPTMVAIADPDAANVARTRLSNLTEILSGPNALQDLVSKTDADLVLVAVVGIA
                     GLRPTLAALKSGREVALANKETLVAGGAAVTEELHRHGSLLPVDSEHSAIAQCLRGER
                     ADSVRRIILTSSGGSLLRRPLETMDQVRIDEALAHPNWKMGKKITVDSATLMNKGLEV
                     IEAHWLFGLTPDQIDVIIHPQSIVHSMVEFVDGSVKAQLGRPDMKTFIAYALYGGERP
                     ALPSTPLEWARVQLSFERPDPQRFPALGYAYEALRSGGTMPAVMNAANEVAVQLFLEG
                     RIKFPDIPRIVRTAMDAHKAIAGPSVEQILDADVWAREYTNYVRSDIVA"
     gene            4032..5063
                     /locus_tag="AC159B_04750"
     CDS             4032..5063
                     /locus_tag="AC159B_04750"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HIR3377088.1"
                     /translation="MPNIVLAVIAFGVMIFVHELGHFLVAKRVGVTVYAFALGFGPRL
                     LGFRRGDTQYAINLLPFGGYVRMAGEDLDDPGGPGSFRSKSVWQRMAVVAAGPGMNLL
                     LAFVLLTSMALAVGVPVGVSNRVGQLLSGWPAEQAGLRQGDAIVAINGVPMRDGRQIV
                     DTIHKHPNERLVLTVDRNGQEFTIAVTSRLEPRQKIGLIGFSPEPIRERMNPVRAFAW
                     GVQNTVQYMSVILTTLVQLVRDRQMLEQLGGPVAAVDFLGDAASAGSEIFVYTAALFS
                     IMIGLFNLIPFPALDGGRLSFLVVEAFRRRPLDPKREGYIHLVGFAVLFLLLIILTGQ
                     DVIRIWFRE"
     gene            5056..>6059
                     /gene="ispG"
                     /locus_tag="AC159B_04755"
                     /gene_synonym="gcpE"
     CDS             5056..>6059
                     /gene="ispG"
                     /locus_tag="AC159B_04755"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HIR3377089.1"
                     /translation="MNSAKRQQTRKIRVGNVEIGGGAPISVQSMTITDTRDVEGTLVQ
                     IYQLATEGCEIVRLAVPDQEAAGALAEIKPRSPLPIVADIHFDYRLAISALEAGVDKL
                     RINPGNIGSRARTRIVAKAAKERGVPIRIGANIGSLSKAMLRRFREPSAEALVESAME
                     DVQVLEDLDFHDIVISVKASEVPMAVRAYTMISERTRYPLHVGITESGTAWAGGIKSA
                     TGIGAILSSGIGDTIRVSLAAHPVEEVKAGFEILKSLGLRTRGVQLVACPSCGRAEVD
                     IISIAQEVERRLARVNAPVKVAVMGCAVNGPGEARMADLGIACGRGMGLLFKDGKIIA
                     "
BASE COUNT         1073 a         1856 c         2028 g         1102 t
ORIGIN      
        1 gcatgctggg aacggccatc aacgccctgg ccttgcaggc cgtgatggaa ggccgggggc
       61 ttgtgacgcg ggtgcagacc gcgatcgaga tgcaccagat cgcggagccg tttatccgcc
      121 gccgcgcgat gcggcacctg gagaaaggcc gcgtcgtgat cttcgccggc ggaaccggca
      181 gcccgtattt caccacggac accgccgccg cgcttcgggc catcgagatt gaggccggag
      241 cgctcctcat ggcgaagaag ggcgtggacg gcgtgtacga taaagacccg aacgtacatc
      301 ccgacgccgt gatctatcgc cggctcggtt acctcgacgt tcttagtcag gacctcaagg
      361 tcatggacgc caccgccgtc gcgctgtgca aggacaacaa tatggacatc atcgtctttg
      421 acgtcgcgca gcccggcaac gtcaaacgcg ctgtgctggg cgaagaagtg ggcacgctgg
      481 tcacgtcctc ggggcgcgcg cgatgatcgc ggacgtcatc agcgacacca ggtcaaggat
      541 ggcgaaggcg atcgacgcga ccaagcatga gttctcctcg ctgcgcaccg gccgcgccag
      601 cccggcactt ctcgagcaga ttcgtgtcga ctactacggt acccccaccc cgatcagcca
      661 gatggcgaca atcacggtgc ccgagccgcg gctgttggtc gtccagccct gggaccgcaa
      721 gatggtgaaa gacatcgaac gggggattct gaagagcgag ctgggactgg tcccgtcatc
      781 ggacggcacg catgtccgcg tgccgattcc gtcgttgacc gaagagcggc gtaaggaact
      841 ggtcaagatc gctcaccgcc acgccgagga gggccgcgtg gccatccgca acgttcgccg
      901 cgaggcgaaa gagatgatcg agcagctcga agaggacggc gatatctctg aagatgacgc
      961 gaagcgcggt ctggacgaac tgcagaagct gacggataag tacatcgccg atgttgaggc
     1021 gctgctcgcg agcaaagaca aggagatcat ggaggtctag gtgatcgacg tgatcgggct
     1081 cgagctcgaa gaggcacggc gccggctcga ggccgcaggg atggtcgtgg cagtatctga
     1141 gactcggtcg cccattccca ctgtgcggct gaccggaccg atgcgtgtcg tcaggcaacg
     1201 tgttacctca gagggagtcg agctcgtcgt gaccgccgaa cggtacgatc ccgcttccct
     1261 gcgggtgaac gcgccccgcc cccgtgaccg atagccgctc cgcagagccg ctctcagcga
     1321 ctcaggcgat tctcgatcca acacgaattc caacacacat cgcgatcatc atggacggga
     1381 acggccgatg ggcctcctcc cgggggttga atcgcgcagc cggccaccgt gccggcctcg
     1441 atgccgtccg caggacgctc gaagcgagcc gtgagctcgg cgtcgccgtt cttaccctct
     1501 atgcattttc aacggagaac tggcgccggc cggccgaaga agtcgaggcg ctgatgcagc
     1561 tcctcggcga ggccgtgtcc gacgaggcgg acgagttgat gcgcaaagga atccagttgc
     1621 gtgtgagcgg cgacctcagt tcgatcgatc ccggcctgcg ccgcgaagtc gagcgcgtgg
     1681 ttgcgctcac caaagacaac ggagacgtca tcctcaatgt cgcgtacaac tacggcggac
     1741 gcgccgagat cctgtccgcg gcgcgcaggc ttgccggtga cgccgccgcc ggacggcttc
     1801 cggccgactc catcgacgaa gcggcgctcg gccggtatct ctacacggcg ggcctccctg
     1861 atcctgatct gctcatccgg acgggcggag agcagcggat cagcaacttc ctcctctggc
     1921 aaattgcgta tactgagctg tatttctgcg acgtatactg gccggacttc gacaaggttc
     1981 acctgtcggc cgcgatccag acgtaccagc agcggctgcg gcggttcggt ggcatcgaaa
     2041 gctcgtaaaa ttctgatgga tcaggtcacg tcgacgttcg ctcgggccaa gcttctccct
     2101 cgcacgatga cggcgttgat cggcggcggt gcatttctca ccgccgcgtg ggcgggcggg
     2161 gtgtggtggg tggccatcat ggcgatcctc gcgctgctcg ccggcatcga atacgccggt
     2221 ctgcatccac aggtcgccgc acggaatgcc gttccggcag gggcggtcct cgcggtcctg
     2281 ttggccgggg ccggcatctg gatggaccaa ttgctgctca tgctcggcgc gttcgtgttc
     2341 ttcggcggcg tagttccctg gatcgcccgt ggaatggacc cgcaccgctg ggctcagccg
     2401 gcaatgctga tactggtcca gggggtcgcc tacgtcgggg tcccgctggg cgtgctggcg
     2461 cgctggcggc tccagggcac attcgatgga gtcctctgga tgctcgtgat cgtgtgggcc
     2521 ggtgacattg ccgcgtactt tgtcgggctc cttgtcggcc ggcacaagat ggcgccaaaa
     2581 gtgagcccgg gcaagagctg ggaaggcgcg gctgccggtc tcgcggcagg agccttcgcc
     2641 ggactgctgg ccgggccggt cttcggcctg ggcccactgg gcgccatcgc gctcggggcg
     2701 atcacgagta tcacggctca gagtggcgac ctgtttgaat cagctctcaa acggcgggcc
     2761 ggcgtcaaag actcaggagg attgcttccc ggtcacggtg gtgtgctcga tcggttcgat
     2821 gccgtatttt ttgcggcgcc ggtcgcgtat ggactgttga aactatgggg acgactctga
     2881 acgagccatg aagcgaatca tcattctggg ctcaacggga tcaatcggtc gtcaggcgct
     2941 ggagatcgtc gatgccgcac ccggtgattt tcaggttgcg gggcttgggg cgcgacgcga
     3001 cgcggagacg ctgaccgcgc aggcgcgcaa gtacaaaccg acgatggttg ccattgccga
     3061 tcctgacgcg gccaacgttg cccgcacgcg cctttcgaac cttacagaga tcctgtcggg
     3121 acccaacgcc cttcaggatc tcgtctccaa aactgacgcc gacctagtgc tggtggcggt
     3181 ggtagggatt gccggactgc gccccacgct ggctgcgctc aaatcggggc gcgaggtcgc
     3241 gctggccaat aaagagacgc tcgttgccgg gggcgctgct gtcacagaag aactgcaccg
     3301 gcacggctca cttttgcccg tcgacagcga gcattctgcg atcgcgcagt gcctgcgggg
     3361 agagagagcg gactcggtgc ggcggatcat cctgacgtct tccggaggat cgctgttgcg
     3421 ccgccccctc gagacgatgg accaggtccg gatcgatgaa gctctggccc atccgaattg
     3481 gaaaatgggc aagaagatta cggtggactc ggcaacgctc atgaacaagg gcctcgaggt
     3541 catcgaggcc cactggctct tcgggctgac gccggaccag atcgacgtga tcatccatcc
     3601 gcagagcatc gtgcactcga tggtggagtt cgtggacgga tcggtgaagg cacaactggg
     3661 tcgccccgac atgaagacgt tcatcgcgta cgcactctat ggaggggagc gaccggcgct
     3721 cccgtcgacg cctctggaat gggcgcgggt gcagctgtcg ttcgaacgtc cagaccccca
     3781 acgcttcccc gcgctggggt acgcgtacga agcgctgcgt tccgggggca cgatgccagc
     3841 ggtgatgaac gctgccaacg aggtcgccgt gcagttattc ttagagggcc ggatcaaatt
     3901 cccggatatt ccgaggatcg tccgcactgc gatggacgcg cataaggcca tcgcggggcc
     3961 ttccgtcgag cagatcctcg acgcggatgt gtgggcacgt gagtacacta actatgtgag
     4021 gagcgacatc gttgcctaac attgtccttg ccgtcattgc gtttggcgtg atgatcttcg
     4081 tccacgagct gggccacttt ctcgtggcca agcgcgtagg ggtcaccgtg tacgcgtttg
     4141 cgctggggtt tggtccacgg ctgctcgggt tccgccgcgg cgacacgcag tacgcgatca
     4201 acctcctgcc gtttggaggc tacgtgcgga tggccggtga ggacctcgat gatcccggcg
     4261 gccccggcag cttccggtcg aaatcggtct ggcagcgcat ggctgttgtt gccgccggtc
     4321 ccgggatgaa cctgctgctc gcgttcgtgc tgctcacgag catggcgctg gcggtgggcg
     4381 tgccggtagg ggtcagcaac cgagtcggcc agctcctgtc gggctggccc gccgagcagg
     4441 ccggactgcg tcagggcgat gccatcgtgg cgatcaacgg ggtgccgatg cgcgacggta
     4501 ggcagatcgt cgatacgatc cacaagcatc ccaatgaacg gctcgtgttg acggtcgacc
     4561 ggaacggtca ggagttcact atcgcggtga cctcacgtct ggagccgagg cagaagattg
     4621 gcctgattgg gttctcgcct gaacccatcc gggaacggat gaaccctgtg cgcgcgttcg
     4681 cgtggggtgt gcaaaacacc gtgcagtata tgtcggtcat cctgaccacg ctggttcaac
     4741 tggtgcgcga tcgccagatg ctcgagcagc ttgggggccc ggtggccgcc gtcgacttcc
     4801 tcggtgacgc cgcgtcggcg ggatccgaga tcttcgtgta caccgccgcg ctcttctcga
     4861 tcatgatcgg tctattcaac ctgattccgt ttcccgctct cgacggcgga cggctcagct
     4921 ttctcgtcgt cgaggcgttc cgccgccgtc ccttggatcc gaagcgagaa gggtacattc
     4981 atctggtcgg gtttgccgtg ctcttcctgt tgttgataat attgacgggt caagatgtca
     5041 tcagaatctg gttccgtgaa tagcgcaaag cgccagcaga cgcgcaagat ccgtgtcggg
     5101 aacgtcgaga tcggcggcgg cgcgccgatc tcggtgcagt ccatgaccat taccgacacg
     5161 cgggacgtcg aaggcaccct cgtccagatc taccagctgg ccaccgaagg ctgcgagatc
     5221 gtgcggctgg ccgtcccgga tcaggaggcg gccggcgcgc ttgccgagat caagccgcgc
     5281 agtccgcttc ccatcgtggc cgacatccac ttcgattacc ggctagcgat ttcggcgctg
     5341 gaggcgggcg tcgacaaact gcgcatcaac cccgggaata tcggtagccg cgcccgcacg
     5401 cgcatcgtcg cgaaggcggc gaaagagcga ggcgtcccga tccgcatcgg cgccaacatc
     5461 gggtcgctct ccaaagcgat gctgcggcgg ttccgtgagc cgtccgcgga ggcgctcgtc
     5521 gagagcgcca tggaagacgt gcaggtgctc gaagatctcg acttccacga catcgtcatc
     5581 tcggtcaagg cgtccgaggt gccgatggcg gtcagagcct acacgatgat cagcgagcgg
     5641 acccgttatc cactgcacgt cggcatcacc gagtccggca ccgcctgggc aggcggcata
     5701 aaatcggcga ccggaatcgg tgcgatcttg tcgtccggaa tcggcgatac gatccgggtg
     5761 tccctcgccg cgcatccggt cgaagaggtc aaggcggggt ttgagattct caagagcctg
     5821 ggactccgta cgcggggcgt gcaactcgtc gcctgtccat cgtgtggcag ggccgaggtc
     5881 gacatcatct cgatcgctca ggaagtcgag cgacggctgg cccgcgtcaa cgcgccggtg
     5941 aaagtcgcgg tgatgggctg cgccgtcaac ggacccgggg aagcacgcat ggcggacctg
     6001 gggattgcct gcggccgggg gatggggctg ctcttcaagg atggcaagat catcgccag
//
LOCUS       DBNGSB010000165         5879 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3643973, whole genome shotgun sequence.
ACCESSION   DBNGSB010000165 DBNGSB010000000
VERSION     DBNGSB010000165.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5879)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3643973"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="AC159B_04760"
     CDS             complement(<1..447)
                     /locus_tag="AC159B_04760"
                     /inference="COORDINATES: protein motif:HMM:NF015644.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HIR3377090.1"
                     /translation="MRIRLSLLAMLFVFFILAPTLARWYTDWLWFGEVGYRRVFWVPL
                     LSRIAVTVVTGGVLFALFLVNLRPLLRRPALDDVIDLEPRSRGSREFRRVLRRQQIGG
                     LVMVVLGLAAFLSGLAASTRWPMFQQFVHTRPFGATDPIFGRDIGFF"
     gene            503..835
                     /locus_tag="AC159B_04765"
     CDS             503..835
                     /locus_tag="AC159B_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008631806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HIR3377091.1"
                     /translation="MSTTADSDTITKEQAVQALQTVFDPEIPVNVWDLGLIYDLQVQE
                     NDVAIKMTLTAVGCPIGPQIASEIENKLQSVGAEKVTVEFVWSPPWSPGRLTEDGKLA
                     LQSMGYPV"
     gene            935..1291
                     /locus_tag="AC159B_04770"
     CDS             935..1291
                     /locus_tag="AC159B_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PleD family two-component system response
                     regulator"
                     /protein_id="HIR3377092.1"
                     /translation="MFVLVFDDNLLSSASLLSQLQSTGHDAIAVNTAVEARRAVPRRP
                     DALLINLMARAFDPYSLIHELREDPAMQDTPIVGFCGHLDELRKKSALEAGCHHIISN
                     AQAHKQLVATLQQLLV"
     gene            complement(1266..2699)
                     /locus_tag="AC159B_04775"
     CDS             complement(1266..2699)
                     /locus_tag="AC159B_04775"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0006541 - L-glutamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate/hydrogenobyrinate a,c-diamide
                     synthase"
                     /protein_id="HIR3377093.1"
                     /translation="MHIPRIIIAGTHSGSGKTSVTLGVLRALKRRRLAVQPFKAGPDF
                     IDPSLHSVAAGRVSRTLDSWLLPHSTVKELCARAAEGADVAVIEGGMGLFDGYHGGGE
                     EGSTAEIAKWLAAPVVLVVDAGGAVRSVAATAMGFSAFDPDLTIAGVIANRVGSELHR
                     AWLHDSLAAAGTRLLGTLPWDARLALPERWGPREIRGREFLGWQRHLGLVPAQEHDAE
                     GAVAALGDAAETHIDLDLILRIARQAPPLVVPGPLVFPLMSIPADVRIGVAQDAAFHF
                     YYPDALELLESRGAKVIPFSPIADSSIPDVDGLYLGGGFPEVYAEQLSANRSMQDSVL
                     KAIDRGMPVYAEGGGLMYLCRDIIDQNATRHEMVGAVAATAQTHARLVALAYVTLEAD
                     DDTLLLRKGEQARGHELHFSTVTMDEPEPLAFRSLEGRGINGGRDGIARGNLLATLTH
                     LHFAGTPMLAERFVEACRQFKRAVVGA"
     gene            3188..4576
                     /locus_tag="AC159B_04780"
     CDS             3188..4576
                     /locus_tag="AC159B_04780"
                     /inference="COORDINATES: protein motif:HMM:NF021507.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HIR3377094.1"
                     /translation="MRYRHLFIWLGIALLLTPSLSAESAPTQVTDVSWEADGLVGKVV
                     IRLNGAFAYRTTASRSSIRIDLWRAQHTQWQSLAVDHPYVRRVHVNQITSEVARLRID
                     LKKPARYKTFVRTDPSMLAVQIIPPWMATRRLPASLAYEKLRVPTGTGTTAVHVLRVN
                     PQDPSLEIRPVLASDMVIGKETTSIIATRHDAVAGINGGYFAGTGLPLGMVVIDGELI
                     SAPLPRRSVFAISRSGKPVIQPFEFQGRIMTSDNTSLLISSVNRPPVAGGLAVYTPAY
                     GPLTPPFRLAAIVRNGVIDSLTSGRILIPEDGYVLVVNEGDAGLLLQHVQTGQRVWMK
                     LNFTQDLEVISALGGGPRLVKDGAEFIPFDWEWFSSRLSQNRAPRTAIGITAAEKLLF
                     VTVDGRSKQNTGMTLRELAQLMVRLGSHHAMNLDGGGSATMVVGGRIMNDPSDGRERP
                     VTSALIVLHRSR"
     gene            4721..>5879
                     /locus_tag="AC159B_04785"
     CDS             4721..>5879
                     /locus_tag="AC159B_04785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3377095.1"
                     /translation="MQLHADVLVVGAGPAGSATAIQIARHGHRVILVDRAKFPRDKPC
                     GDYCDPGAVHALDQLGCLPEILAAGAAIIPGMRIVAQDGAELSADFPEGRGLLLPRVR
                     LDYILLRRAASAGAEIVEGFRVDEVAAGASGVEIRDSHGKQLRASLLVAADGMRSVTA
                     RRLGLYSEIASGRFTVGAYFEVPETPARGELHLGSGLYCGVARFGGGNANVCMALPRD
                     RLKGRTSPQAFADSLAHLPALAADLAGARRTSDFRSSGQGGFRASRVVADRVLLAGDA
                     AMQIDPMTGQGICFALRSGILAAETAARALTTKNFSRQGLREYARRRAAEFGRKVTLA
                     RLLQSLALRPTLTPRLVTRLGARPEQASQLLGATGDILAANSVLHPHYIWNL"
BASE COUNT         1165 a         1837 c         1793 g         1084 t
ORIGIN      
        1 gaagaaacca atatcgcgcc cgaaaatcgg atcggtggcg ccgaacggcc gcgtatgaac
       61 aaactgctgg aacatcggcc atcgggtcga ggcggcaagg ccggagagga acgccgccag
      121 gcccagaaca accatgacca gcccgccgat ctgctgcctg cggagcacac gcctaaactc
      181 ccggctgccg cggctccgcg gctcgagatc gatcacatca tccagcgcgg gtcgccggag
      241 cagcggcctc aagttcacga gaaagagggc gaacaagacg ccaccggtga cgactgtgac
      301 ggcgatccga gacaacaacg gcacccagaa aacgcggcgg tagccgactt caccgaacca
      361 gagccagtcc gtgtaccatc gggccagcgt cggcgcgagg atgaagaaga caaacagcat
      421 tgcaagaaga gatagcctga tgcgcatgac ggtaggtagg ttctaggggt tgcgccccag
      481 ggcctgtgct acaatcgaac gcgtgagtac aacagccgat tccgatacga ttaccaaaga
      541 acaagccgtc caggccctgc agacggtgtt cgatccggag attccggtaa acgtctggga
      601 tcttggactc atctacgatc tgcaggttca ggagaacgac gttgccatca agatgaccct
      661 cactgccgtc ggctgtccta tcgggccgca gattgcgtct gagattgaga acaaactgca
      721 gtcggtcggc gccgaaaaag tcacggtcga gtttgtgtgg agcccgccct ggtcgcctgg
      781 gcgcctgact gaagacggca agctggcact gcagagtatg ggatatcccg tataactacg
      841 ttcgtaggtc gtgggtcgta ggtcgtgggt cgttgctcag tcagggcagg ttctacgacc
      901 tatgacctac gaccgacgac cgtagtttgg taccatgttt gtcctcgttt tcgacgacaa
      961 tctcctctcc tcggcttctc tactcagtca gttacagtcc accgggcacg acgcgatcgc
     1021 ggtcaacacc gcggtggaag cacgccgcgc ggttccgagg cggccagacg cgcttctgat
     1081 caacctcatg gcccgcgcgt tcgatccgta ctcgcttatc cacgagctgc gcgaagatcc
     1141 cgcaatgcaa gacacaccga tcgtgggatt ctgcgggcac ctggacgagc ttcgaaagaa
     1201 aagcgcgctc gaagccggct gccatcacat catcagcaac gcccaggcgc ataaacagct
     1261 cgtggctacg ctccaacaac tgctcgtttg aattgcctac acgcctccac aaaccgttcc
     1321 gcaagcatcg gggttccggc gaagtggaga tgcgtcaacg tggcgaggag attcccgcgg
     1381 gcgataccgt cccgtcctcc gttgattccg cggccttcga gcgatcgaaa ggcaaggggc
     1441 tcaggctcgt ccatggtaac tgtcgagaag tgcaactcgt gccctctcgc ctgctcgcct
     1501 ttgcgaagaa ggagcgtatc atcatcggcc tcgagcgtga cgtaggctag agcgacaagc
     1561 cgggcgtgcg tctgcgccgt tgcggccaca gctccgacca tttcgtgcct ggtcgcattc
     1621 tgatcgatga tgtcgcggca caaatacatg agccctccgc cctccgcata gacgggcatg
     1681 cctcggtcga tcgctttgag cacgctgtcc tgcattgatc ggtttgcgct gagctgctcc
     1741 gcatacacct cggggaaccc gccgccgaga tagagcccat ccacatcggg aatcgaagaa
     1801 tcggcgatcg ggctgaacgg aatcactttc gcgccgcgtg attcgagcaa ctcgagagcg
     1861 tcagggtagt agaagtgaaa cgccgcgtct tgcgccaccc cgatccggac atcggccgga
     1921 atggacatca gtggaaacac cagcggaccg ggcacgacca gaggcggcgc ctggcgggcg
     1981 atgcgaagga tcaggtccag atcgatatgg gtttcggcgg catcgccgag cgccgcaact
     2041 gcgccttcgg catcgtgctc ctgcgcaggg acgagaccga ggtggcgctg ccaccccaga
     2101 aattcgcggc cgcgaatttc ccgggggccc cagcgctcgg gaagagcgag acgcgcgtcc
     2161 cagggcagtg tgccgagtag ccgcgtcccg gccgccgcca gcgaatcgtg aagccacgcc
     2221 ctgtgaagct cgcttcccac gcgattcgcg atcacgccgg cgatcgtcag gtcgggatcg
     2281 aacgccgaga aacccatggc cgtggccgca acgctgcgga ccgcgccccc ggcatcgacg
     2341 acgagcacca cgggcgcggc cagccactta gcgatctccg cggtactgcc ctcctcgccg
     2401 ccgccgtggt atccatcgaa taatcccatg ccgccctcga tgacggctac atccgcgccc
     2461 tccgcagcac gtgcgcagag ctccttaacg gtcgaatgag gcaacagcca tgaatccagc
     2521 gttcgcgaca cacgccctgc ggcgactgaa tggagtgatg gatcgataaa atccggccct
     2581 gccttgaatg gctgcacggc caggcggcgg cgctttaacg cgcgcagtac gccaagggtc
     2641 accgatgtct tacctgagcc gctgtgagtt ccggcgatga tgattctggg gatgtgcatt
     2701 ggaatgaaaa ccacttcgac atgcggcggg acggggacct tggaccgggg acccggcaat
     2761 cggctatgga gtgacttagt tacgggtccc ctttctccaa ggtccccatc ccgctaactc
     2821 tattgacagg acctttgatg aaccgatatg ctggacaggt tccagctact tcaagtggct
     2881 gagaagagag gaggtgaggc cgacgatgcg tagaatgacc ccggcccacc ggagaggatt
     2941 ggcaggttaa ggacaggttc tgaatctgtc ctagcgtcct ggcttgtggg ccggggcgct
     3001 tttcttttaa aggggatagt cgacccttca cgaactccag ttcgctaggt ctcgctggct
     3061 aatcggcggg gtacgtacct gacgtcacac cttatacgaa cccctcgggc cgcgtgccgg
     3121 ggcccccgga aggtcgcact cgtaaatctc tggggaggca ccagcggtcc caaaacggag
     3181 cgacatcgtg cgctatcgac acctgttcat ctggctcggc attgcccttc tactgacccc
     3241 ttcgctatcc gctgaatccg caccgaccca ggtcactgac gtctcctggg aagcggatgg
     3301 cctggtcggc aaggtcgtca tacgcctcaa cggcgccttc gcgtatcgga cgacagcgtc
     3361 gcggtcgagc atccggatcg atctctggcg cgcccagcac acgcaatggc agtcgctggc
     3421 cgtggatcat ccgtatgtcc gccgcgtcca cgtcaaccag attacgagcg aggtcgcgcg
     3481 cctgcgcatc gacctcaaga agccggcacg ctacaagact ttcgtgcgaa ctgatccctc
     3541 gatgctcgcc gtgcaaatca ttcctccatg gatggcgacg aggcgtttgc cggcaagcct
     3601 ggcatatgag aagcttcgtg tcccaactgg gacgggcacc acggcggttc atgtcttgcg
     3661 tgtgaacccg caagatccca gcctcgagat ccggccggta ctggcctcag acatggtcat
     3721 cgggaaggaa accacgagta tcatcgccac ccgtcacgac gccgtcgcgg gcatcaatgg
     3781 cggttacttc gccggaaccg gactcccgct tgggatggtc gtcatcgacg gcgaactcat
     3841 ctctgctccg cttccccggc ggtcggtgtt tgccatttcg cgctccggca agcccgtcat
     3901 tcaaccgttc gagtttcagg gacggatcat gacatcggac aacacatcgc tcttgatttc
     3961 ttctgtgaac cgtccgccgg tggccggagg cctggccgtg tacacaccgg cttacggtcc
     4021 cctgacgcct ccgttcaggc tggcggcgat cgtcagaaac ggcgtgatcg acagtttgac
     4081 ctccgggcgc atcctgatcc ccgaagacgg ttatgtgctg gttgtcaatg aaggcgacgc
     4141 cggcctgctc ttacagcacg tgcaaaccgg tcagcgggtt tggatgaaac tcaacttcac
     4201 tcaggacctc gaggttatca gcgccctcgg cgggggcccg aggctcgtca aagatggcgc
     4261 tgagttcatt ccgttcgact gggaatggtt ctcgagccgc ctgtcccaga accgcgcgcc
     4321 gcggacggcc atcggaatca cagccgcaga aaagttgctg ttcgtcaccg tcgatgggcg
     4381 cagcaaacag aatactggta tgacgctacg tgaactcgct cagctaatgg ttcgtctagg
     4441 atcacaccac gcgatgaacc ttgacggagg cggctcggct accatggtgg tcggcggccg
     4501 catcatgaat gatccctcag acggccgcga gcgacccgtc acctccgcac tgatcgtcct
     4561 gcacagaagc cgctaacccc gcgagcaagg cgaacaaagc gaactaagcg aacacagtaa
     4621 atctattatc gtgaccttgt tcgctatgtt cgcctcgttt accatgttcg cgtagtttgt
     4681 tgttacactg tcgtcacatc gccaaaggta aatacgtatc gtgcaattgc acgctgatgt
     4741 tcttgtagtg ggagccggac cggccggaag cgccacggca attcagatag cccggcacgg
     4801 ccaccgggtc atcctggttg accgggcgaa gttcccgaga gacaaaccgt gcggcgacta
     4861 ttgcgatcca ggtgcggttc atgcgctcga ccagcttgga tgcctgccgg aaattctcgc
     4921 agcgggtgcg gcaatcattc ccgggatgcg gatcgtggcg caggacggcg cggaactgtc
     4981 ggcagacttc cccgagggcc ggggactgct gttacctcgc gtgcggctgg attacatact
     5041 actacggcga gcggcttccg ccggcgcgga aatcgtagaa gggtttcggg tcgatgaggt
     5101 tgcggccggc gccagtgggg ttgaaatccg ggattcccac gggaaacagc tcagggcttc
     5161 gttactcgtc gcggcggacg gcatgcgctc tgtgacggca cgccggctgg gtctctattc
     5221 ggaaatcgct tccggccgct ttacggtcgg tgcgtatttc gaggtccccg aaacgccggc
     5281 gcgcggcgaa ttgcatctgg gatcgggact ctattgcggg gtcgctcggt tcggaggcgg
     5341 caacgccaac gtgtgcatgg cgcttcctcg agaccgcctc aaaggacgca catccccgca
     5401 ggcgttcgcg gattcgctcg cacatttgcc ggcactggcg gccgacctcg ctggtgcgcg
     5461 ccggacctct gacttccgct ccagtggcca gggggggttc cgagcctcac gggtcgtagc
     5521 cgatcgcgtg ttgctggccg gcgacgcggc gatgcagatc gacccgatga ccggccaggg
     5581 tatctgcttc gccctgcgct ccgggatcct ggcagccgag accgcagcgc gcgccctgac
     5641 gacgaagaat ttctcgcggc agggactgcg agagtacgcg cggcggcgcg ctgccgagtt
     5701 tggacgaaaa gtcaccctcg cccgactgtt gcaatcgctc gctctgcggc cgaccctgac
     5761 acccagacta gtgacacgac tgggcgccag accggaacag gcgagtcaat tactcggcgc
     5821 gaccggtgac atcctggctg cgaacagcgt cctgcatccg cattacatct ggaacctat
//
LOCUS       DBNGSB010000166         9496 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_4258906, whole genome shotgun sequence.
ACCESSION   DBNGSB010000166 DBNGSB010000000
VERSION     DBNGSB010000166.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 9496)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9496)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9496
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_4258906"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="AC159B_04790"
     CDS             complement(<1..750)
                     /locus_tag="AC159B_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002627766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3377096.1"
                     /translation="MSDVVIQVRNLRRALGGAQVLSDVSFEVRQGEIFVIMGPSGTGK
                     SVLLKHLIGLLRPDAGEIFLWDIPIHERSNEELDEVRKRIGVVFQGAALFDSLAVGEN
                     IAFPIRRHRDLEEHAVQEAVATRLALVGLEGKEEAMPATLSGGMRKRVGIARAVALNP
                     EIVFYDEPTGGLDPPTARDVDSLIWRLRAYLGMTSVVVTHDLDSAFGIGDRIALMGRG
                     RLIAVGTPEEIEADKRPEIREFLHPRRIVRAE"
     gene            complement(760..1542)
                     /locus_tag="AC159B_04795"
     CDS             complement(760..1542)
                     /locus_tag="AC159B_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007958909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family ABC transporter permease"
                     /protein_id="HIR3377097.1"
                     /translation="MAAIQQVKGLDRFGRGVLGFLEEAGRVVLLFSSTLRWVLRGVLE
                     WRQTVLQMARIGVESLPIILVTGTFAGMVLAFQTSKQLIALGAPGFIGGAVAVSMARE
                     AAPVFTAITVAGRISAGIAAELGTMAVTEQIDALRVMATNPVRFLVVPRVLAGVTMQP
                     LLTIFANVAGLVGGGVIAALAGVQLETYGGSVQRFLRLYDFMCGLLKAAAFGIIITMI
                     GSYRGLATSGGAEGVGRAATAAVVVSIVLILVTNYFLDVVLF"
     gene            complement(1692..1780)
                     /locus_tag="AC159B_04800"
     tRNA            complement(1692..1780)
                     /locus_tag="AC159B_04800"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1744..1746),aa:Ser,seq:cga)
     gene            complement(1807..3126)
                     /locus_tag="AC159B_04805"
     CDS             complement(1807..3126)
                     /locus_tag="AC159B_04805"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3377098.1"
                     /translation="MAEPRTARTPVWLKRLGNRIRQVRRYRGLTQTDLAQPDLTKSFI
                     SLLESGRTYPSVGTLVALASRLQTSLALLLLEDSELPRETTLTLVSLARSLAGRNPDL
                     VDHLLATAADLSAESNDLRAEIMLTRGDIAIAQGRTPDADRLLNEALSWAHRHRLKAH
                     EPRILTRLASLAQQRGDVGAARTNLEEALGQFRATRTLRSIDGCDAMLAYADTLIEQG
                     KPARALRALQDVARVAERQDLPSVLGRAHIGMARAHLQVGRGQEASDALRQAKSALEG
                     VTESTDLTRTLRNLAALQTMAGHHQDAYASLQQAMRLVENLPDIRLRAAVVVDLARAL
                     AQVGRHDDAADAAKSALDLLRSHPDAYLRGQLLVTLARVSRAQKRGKQALDYFKEAVA
                     IFKKGRRQVELAETARELGMFLKERGDHADAADYLAMAISVEKARAN"
     gene            complement(3199..3537)
                     /locus_tag="AC159B_04810"
     CDS             complement(3199..3537)
                     /locus_tag="AC159B_04810"
                     /inference="COORDINATES: protein motif:HMM:NF013868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR3377099.1"
                     /translation="MADLNGVPVAALEGEIDIANATQLRAQLTAAIPNTAAAFVIDLT
                     PTSYLDSRGVHLILEVAERAARGQQLIRLVVPRDSHVYRILELSRVQDAVPIDATVDD
                     AVLAITKAKP"
     gene            complement(3488..5104)
                     /locus_tag="AC159B_04815"
     CDS             complement(3488..5104)
                     /locus_tag="AC159B_04815"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding SpoIIE family protein phosphatase"
                     /protein_id="HIR3377100.1"
                     /translation="MAAETRILIVDDDPALLQALPETLRLRIDGVVVDTADSAAAALE
                     RIKAVDYDAVVTDIKMPGMDGLALLNEIRSLRPETPTLLITGHGEHDLAVQALRGGAY
                     DFIAKPIERDYFAASLNRAIRTRQLKRELDAQKTELEEHTKQLERALRMYEREHSIAE
                     TLQRSLLPRRLPDIPGITAAARYLPATPEAVGGDWYDMFSLPGGRVGVVMGDVAGRGI
                     QVASVMGQLRNALRAYAGEGYSPSVVLSRLASIIEPTEMATLLFMVFDPSSWKIEYAN
                     AGHPPPLVATPDGHASFLEGGSLPLGPALNQTYRDLTVDIAPGSLLMLYTDGLVEDRS
                     TDLNESLHRLRDTVMRSVNGDVEHLADHIVNEIRSTSAGSDDTALLILRIDALDPSKF
                     ALTIPAIPSSLPAIRHALRRWMADSGVETDDAYEVLVASCEACSNVIEHAYGLSSGDV
                     DVHATMVNDMIEVTVRDSGTWTPPRGVHRGRGLHMIRELTDDVEIRHTEHGTIVRMRY
                     RRRGSRNDVDRADQGGGSQRGPRRGSGRGN"
     gene            complement(5094..6215)
                     /locus_tag="AC159B_04820"
     CDS             complement(5094..6215)
                     /locus_tag="AC159B_04820"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3377101.1"
                     /translation="MSALAAAPRAHADEIARLQEHVVVLEQHLHKRDEQRRALLHILS
                     DLHDLNRRLSNQRKAMLHILADYEEDRRRLGSQAERADNSRRALLHILQDAHRSNLKL
                     EDGRKAMIHIMGDLHETTEEIKRREQELREKQEQLVQAGKLATLGELTTGVAHELNNP
                     LNNIGLFLGNSIDLIEMGQVDTTRILRDLRSASQQVRKASEIISHLRTFGRAAPVGHE
                     SVAINQVIHRALSLLQEQFRIRQITIELDLDPAEPHVNGSPIQLEQVFINMLANARDA
                     LTDAPRKLLTISSAVAEDFVIIVFSDSGPGVPKGLEQRIFDPFFTTKEVGEGTGLGLS
                     ITYGILKDHKGTISLDTSVKDGATFRIELPLAVALQHGG"
     gene            complement(6215..7195)
                     /locus_tag="AC159B_04825"
     CDS             complement(6215..7195)
                     /locus_tag="AC159B_04825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR3377102.1"
                     /translation="MVKRRGTLVKNQGIAVDGDRSFTPLTADADQARAYAESLIKNAP
                     DPVFVSDLEGKILQANEAVYNLLGFRPDEVIEQSVSRFISPAETREFTAALREVVEKG
                     VTRNARLNPRSATGEVIPTTLNASALRDPEGHVIGAIGVLRDMRELDKARAYAESLIK
                     NAPDPVFVSDLEAKILQANDAVFALLGFRPDELIEQSLLRFISPEETREFTAALREVV
                     EKGVTRNVRLNPRTAAGEVIPTTLNASALRDPEGRVIGAIGVLRDMREYEQVVRDLEK
                     SKLELQEKILDLEKFEEVVVGRELKMIALEKELESLRREIERLRVGGTFD"
     gene            complement(7309..7833)
                     /locus_tag="AC159B_04830"
     CDS             complement(7309..7833)
                     /locus_tag="AC159B_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HIR3377103.1"
                     /translation="MLAAVRLFAVSAILSVCLFAGTTSLPFIIARLGTELPFRIYSTP
                     TIAAVALEPDGRLVIHLTSRLLRYEHADMLAFVYLHELGHVRLGHLLPQGQRTQFFST
                     SLWRTQAWRMEYDADEWAAGKLRRLGYDPQRGIALTFGVFGDGGGFTHPPDRARIDRV
                     RNLSAPSYPKAEHR"
     gene            complement(7986..8684)
                     /locus_tag="AC159B_04835"
     CDS             complement(7986..8684)
                     /locus_tag="AC159B_04835"
                     /inference="COORDINATES: protein motif:HMM:NF016176.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HIR3377104.1"
                     /translation="MNRSLLILLAVLGAGLTLAYLVSTAAPERVSTPPNPQNQVSAPT
                     GASTAAPSGTQRFVIVPSQSTVTYRVDETLFREGNRIQTAVGTTSAVRGEVIVNRARP
                     AESRIGPVTVDISQFKSDSDRRDNAIKERWLESAKFPMAQFTVTSIKGLPSTYQDGRE
                     IPVEFSGNLKVRDITRPVTWTGTVRLDGDRLTATGNTTIKMTDFGFDPPAILFLKTEN
                     DARLEFRLIATRQG"
     gene            complement(8776..9204)
                     /locus_tag="AC159B_04840"
     CDS             complement(8776..9204)
                     /locus_tag="AC159B_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3377105.1"
                     /translation="MATFMFRFKVLLASGLVVAVLAGLLMISSALAQQGPPPGARRAQ
                     QCQSVISQDAVNPVLARHRDRLQTARESMGREERALRTLLVADTTTRGAVDAQIAKSN
                     DARNAFARARLDLLWDLRSVIPVQNREQAFRCAELLMRRR"
BASE COUNT         1722 a         2930 c         2998 g         1846 t
ORIGIN      
        1 ctcggcgcgc acgatgcgtc gtgggtgcag aaactcacgg atctccggcc gcttgtcggc
       61 ttcgatctcc tccggcgtac cgaccgcgat caacctgccg cggcccatca acgcaatgcg
      121 atccccgatg ccgaaggcgc tgtccagatc atgtgtaacc accaccgacg tcatgccgag
      181 gtacgcgcgc agtcgccaga tgaggctatc gacgtcgcgg gccgtggggg gatcaagccc
      241 acccgtcggc tcatcgtaga agacaatctc cggattgagc gcaacggcgc gcgcgatgcc
      301 gacgcgtttg cgcattccgc cgctcaaagt ggccggcatc gcttcttctt taccctccag
      361 gcccacgagc gccagccggg tcgccacggc ttcctgaacg gcgtgttctt cgaggtcgcg
      421 gtgacggcgg attgggaagg cgatgttctc tccgaccgcg agcgaatcga acagcgcggc
      481 gccctggaac accacaccga tccgctttcg aacctcgtcg agctcttcat tggaccgttc
      541 gtggatcggg atgtcccaga ggaatatttc gccggcatcg ggccggagga gtccgatgag
      601 gtgcttgagc agcacgctct tgccggttcc gctgggtccc atgatcacaa atatctcacc
      661 ttgtctgacc tcgaaggaca cgtctgaaag cacctgggct ccgcccagtg ctctacgaag
      721 attgcggacc tgaatcacta cgtcgctcat cgcttgagtt cagaagagga ccacgtcgag
      781 gaagtaattc gtcacaagga tcaagacgat cgatacgacg acggcggccg tggcggcgcg
      841 cccgacccct tcggcgccac ccgaagtggc caggccccgg tacgagccga tcatagtaat
      901 aatgatccca aaagccgccg ccttcagcag cccgcacatg aaatcgtaga gacgaaggaa
      961 gcgctgcacc gatccaccgt aggtttccag ctgtacgccg gcgagcgcgg cgatcacgcc
     1021 gccgcccacc aggccggcga cgttcgcaaa gatcgtgagg agcggctgca tcgtcacccc
     1081 ggccagcacc ctcggcacaa ctagaaaacg aacgggattt gtggccatca cccgcagggc
     1141 gtcgatctgc tccgtgacgg ccatcgtgcc gagctccgct gcgatgcccg cgctgatccg
     1201 gcccgcgacc gtgatggcgg taaacacggg agccgcttct ctggccatcg ataccgcaac
     1261 cgcgccgccg atgaatcccg gcgcgcccaa cgcgatcaat tgttttgacg tctgaaacgc
     1321 gagcaccatg ccggcaaacg tgccggtgac caggatgatc ggaaggctct cgacgccgat
     1381 gcgggccatc tgtagtacgg tttggcgcca ctcgagcacg ccgcgcaata cccaccggag
     1441 tgttgaagag aagaggagga ctacccggcc tgcttcctct agaaatccga gaacgccgcg
     1501 gccgaatcga tcaagaccct tgacctgctg gatggcggcc ataaccggta ccagagatat
     1561 ttcccctgcg ggcgcaatgg gcgaagggac gtcaaagccc ttcgactcac ttcgttggct
     1621 cagggcattc gaccctgcat tccaatggtc gaagaccatg agcgagcctg ccggtcggca
     1681 ggcgagtcga atggcggagg gggtgggact cgaacccaca aggggctttc accccgccgg
     1741 ttttcgagac cggtcccttt ccagttcggg gcacccctcc aagagaaact ccttgccgat
     1801 tataccttag ttagcgcggg ccttctctac agagatcgcc atcgcgaggt agtcggcggc
     1861 atcggcgtgg tcgccgcgct ccttgaggaa cattcccagt tctcgagccg tctcagccag
     1921 ctccacttga cgcctgccct tcttgaaaat ggcgacggct tccttgaagt aatccaaggc
     1981 ctgctttccg cgcttctgcg cgcgcgagac gcgcgccaat gtgaccagga gttggccgcg
     2041 caagtatgca tccggatgcg atcggagcag atccaacgct gacttggctg cgtccgccgc
     2101 atcgtcatgg cggccgactt gcgccagcgc cctcgccagg tccaccacga cagccgcccg
     2161 cagcctgatg tcaggtaaat tttcaacgag ccgcattgct tgctggagtg aggcatatgc
     2221 gtcctggtgg tgccccgcca ttgtttggag cgcagcaaga ttccgaaggg tgcgcgtgag
     2281 atcggtggat tcggtgactc cttcgagcgc actcttggcc tgccgcagcg catccgaggc
     2341 ctcttgtccg cgcccgacct ggaggtgcgc gcgcgccatg ccgatgtgtg cgcggccaag
     2401 gacagagggg agatcttgac gctctgcgac ccgagcgacg tcctgcagtg cccgcaatgc
     2461 gcgcgccggt ttcccctgct cgatcagcgt gtcggcgtag gccagcatgg cgtcgcaccc
     2521 atcaatggag cgtagtgtcc gtgtggcgcg aaattgcccc agcgcttcct cgagatttgt
     2581 ccgcgcggcc ccgacgtctc cacgctgctg ggccagggag gcgagccgcg tgaggatgcg
     2641 tggctcgtgc gccttgagac ggtgcctgtg cgcccatgac agggcttcgt tcaacagccg
     2701 atcggcgtcg ggcgtccgtc cctgcgcgat cgcgatgtct ccccgcgtca gcataatttc
     2761 tgcgcggagg tcattggact cggccgacaa gtccgcggct gttgccagga ggtgatcaac
     2821 cagatcggga tttcgaccag ccagggagcg ggcgagagag acaagcgtca gcgtggtttc
     2881 ccgcggcaac tcagaatctt cgagcagcag cagcgcgagc gacgtctgca ggcggctggc
     2941 gagtgcgacc agcgtaccca cagagggata ggtccgaccg gattcgagca ggctaatgaa
     3001 gctctttgtc aggtcaggct gtgcgaggtc ggtctgcgtg aggcctcgat accggcggac
     3061 ctgtcggatc cgattgccca gccgtttcag ccagaccggc gttcttgccg tcctgggttc
     3121 tgccatcgtg acggttcgat tctggggcag gggaagcggc ccatttttta cgaggaaagt
     3181 ttaatagtgc gagacagtct agggcttggc cttcgtgatg gcgagcacgg cgtcgtcgac
     3241 cgtcgcatcg atcggcactg cgtcctggac tcgggagagt tcgaggatcc ggtagacgtg
     3301 cgagtcacgc gggaccacca gccgtatgag ttgctgcccc cgggccgcgc gctcggcgac
     3361 ctccagaata agatgaacgc cgcggctgtc gaggtaggac gtcggcgtga gatcgatcac
     3421 gaatgcagcg gcagtgttgg gaatggcggc ggtcagttgc gcccggagtt gcgtggcgtt
     3481 ggcaatgtca atttcccctt ccagagccgc gacggggacc ccgttgagat ccgccacctt
     3541 gatctgccct gtcaacatca ttccgtgacc ccctccgccg gtatcgcatc cggacgatcg
     3601 tgccgtgctc ggtgtgccgg atctcaacgt catccgttaa ttcccgaatc atgtgcaggc
     3661 cgcgtccccg atgtacccca cgagggggtg tccaagtccc gctgtcgcgc acggtgactt
     3721 cgatcatgtc gttgaccatc gtcgcgtgca cgtccacgtc gccgctggac agcccatacg
     3781 cgtgctcgat gacgttggag catgcctcgc agcttgccac caggacctca tacgcatcgt
     3841 cggtctcgac ccccgagtct gccatccaac gacgaagggc gtgcctgatc gcgggcagcg
     3901 aggacggaat cgcagggatt gtgagcgcga acttcgatgg atcaagcgcg tcgatgcgga
     3961 ggatcagcag ggccgtgtca tcgctgcctg cactcgtgga gcggatctca ttcacaatgt
     4021 ggtcggccag gtgctcgaca tccccattca cggatcgcat gacggtgtct ctgaggcggt
     4081 ggaggctctc gttcaggtcg gtgctccgat cttcgacgag tccatcagtg tacagcatga
     4141 gcagcgaacc aggggcgatg tccacggtga ggtctcggta cgtctggttg agcgcaggtc
     4201 ccagcgggag cgagccgcct tcaaggaacg acgcatggcc atccggagtc gcgaccagcg
     4261 ggggagggtg accggcattc gcgtattcga ttttccacga gctgggatcg aagaccatga
     4321 acagaagtgt cgccatctcc gttggctcga tgatggacgc gagacgcgac agcacgacgg
     4381 agggtgaata cccttccccg gcgtaggcgc gcagcgcgtt ccgtagctgg cccatcaccg
     4441 aggcaacctg gatgcctcgc ccggccacat cacccatgac cacaccgacc cgcccaccgg
     4501 gaagggagaa catgtcatac caatcgccgc ccaccgcctc cggcgtggcc ggcaaatatc
     4561 ttgccgccgc ggtgatcccg ggaatatccg gcagccgccg gggaagcagg ctccgctgga
     4621 gtgtttccgc gatgctgtgc tcgcgttcgt acatgcggag ggcacgttcg agctgctttg
     4681 tatgttcttc cagttctgtt ttttgggcat cgagctcgcg tttgagctgc cgcgtccgga
     4741 tcgcccggtt cagcgaggca gcgaagtagt cgcgctcgat gggtttcgcg atgaagtcat
     4801 aagcgccgcc ccgcaaagct tgcaccgcaa gatcgtgttc tccgtggccg gtgatcagca
     4861 gcgtgggtgt ttcggggcgg agcgagcgga tttcattcag aagcgcgagt ccatccatgc
     4921 cgggcatctt gatgtcggtc accaccgcgt cgtagtcgac tgccttgatg cgttccaggg
     4981 cagcggcggc ggaatcggcc gtgtcgacga cgacgccgtc aatacgcaaa cggagggttt
     5041 ccgggagcgc ctggagcagc gcggggtcgt catcgacgat caggatcctc gtctcagccg
     5101 ccatgttgta aggcgaccgc gaggggtagt tcgatgcgga aggttgctcc gtccttaacc
     5161 gacgtgtcca gggaaattgt ccccttgtga tctttgagga tgccgtacgt aatcgacagg
     5221 cctaagccgg tgccttctcc gacctccttg gtagtgaaga atgggtcgaa gatccgttgc
     5281 tccaacccct tcgggacccc agggccgctg tcgctgaaga cgatgatgac aaagtcctcc
     5341 gccaccgccg aggaaatcgt gaggagcttc cgcggagcgt cggtcagcgc gtcgcgcgcg
     5401 ttggcgagca tgttgatgaa gacctgttcg agctggatgg ggctgccgtt gacgtgcggc
     5461 tccgccgggt cgagatccag ctcgatggtg atctggcgga ttctgaattg ttcctgcagg
     5521 agtgagagcg ccctgtggat gacctgattg atcgcaaccg actcgtgccc caccggtgcc
     5581 gcccgtccaa acgtgcgcag gtgcgagatg atctcagacg ccttccgcac ctgctgggag
     5641 gcactgcgca ggtcgcggag gatacgcgtc gtatcgacct ggcccatctc gatgaggtcg
     5701 atgctgttgc caaggaacag cccgatgtta ttgagcgggt tgttgagttc gtgtgcgaca
     5761 cccgtggtga gctcgcccaa tgtcgcgagc ttgcctgcct gcaccagctg ctcttgtttc
     5821 tctcgcagct cttgctcgcg ccgcttgatc tcctcggtcg tctcgtgcag gtcacccatg
     5881 atgtggatca tcgccttgcg gccatcttca agcttgaggt tcgaacgatg cgcgtcttgg
     5941 aggatgtgga ggagggcacg gcgtgagttg tccgctcgct ccgcctgact ccccagccgc
     6001 cggcgatctt cctcgtaatc cgccaggatg tgcagcatcg cctttcgctg gttggagagg
     6061 cgccggttca agtcatggag atcgctgaga atgtggagga gggcgcggcg ctgctcgtcg
     6121 cgcttgtgca ggtgctgctc gaggacgacg acgtgttctt gcagcctggc gatctcatcc
     6181 gcatgggcgc ggggggcggc agccagggcc gacatcaatc gaaggtcccg ccgacgcgaa
     6241 gccgctcaat ttcgcgccgc aggctctcga gttccttttc cagcgcgatc atcttcaact
     6301 ctcgaccgac gacgacttct tcgaactttt ccagatcgag aatcttctct tgcagttcca
     6361 acttggactt ttcgaggtcg cgcaccacct gctcgtactc gcgcatatcg cggaggacgc
     6421 cgatggcgcc gatcacgcgg ccctcgggat cgcggagcgc ggacgcgttc agcgtggtgg
     6481 ggatgacctc gccggcggcg gtgcgggggt tcagccggac gtttcgagtg acgcctttct
     6541 cgacgacctc gcgcagcgcg gccgtgaatt ctcgtgtttc ctcagggctg atgaagcgca
     6601 acagcgactg ctcgatcaac tcatcgggcc ggaagcccag gagcgcaaag accgcgtcat
     6661 tggcctgaag gattttcgcc tcgaggtcgg agacgaagac cgggtccggc gcgtttttga
     6721 taaggctctc cgcgtaggcg cgggctttgt ccagttcacg catgtcgcgg aggacgccga
     6781 tggcgccgat cacgtggccc tcggggtcgc ggagcgcgga cgcgttcagc gtggtgggga
     6841 tgacctcacc tgtggcgctg cgcggattca atcgggcgtt ccgggtgacg cccttctcga
     6901 cgacttcgcg aagggccgcg gtgaactcgc gggtctcggc cggactgata aaccgggaaa
     6961 cggactgttc aataacctcg tccggacgga accccaggag attatagacc gcctcgttgg
     7021 cctggaggat tttgccttcg aggtcggaga cgaagaccgg gtccggcgcg ttcttgatca
     7081 ggctctcggc gtaagcgcgg gcttgatccg cgtctgctgt cagcggtgtg aacgagcggt
     7141 caccgtcgac agcaatgcct tgattcttca ccagcgtgcc tcgccgcttg accatgagat
     7201 tgcgccctct cgcgtagaga tttgggaatt tttttccgtt cgagttctaa ccgaaaccta
     7261 attctaaggg gcggggaaga aacctcgtaa accgaacacg cgttcggctc agcggtgttc
     7321 agccttaggg tagctgggcg cactcaaatt gcggacgcga tcgattcggg cccggtcggg
     7381 tggatgcgtg aacccgccac catcaccgaa aactccaaag gtcagtgcaa tcccacgctg
     7441 cgggtcgtaa ccgagccgcc gcaacttgcc ggccgcccac tcgtcggcgt cgtattccat
     7501 gcgccatgcc tgtgtacgcc acagggaggt ggagaaaaac tgtgtccgct ggccttgtgg
     7561 aagcagatgg ccgaggcgca cgtgaccgag ctcgtggaga tagacgaacg cgagcatgtc
     7621 ggcgtgttca tagcgcagca gcctggatgt gagatgaatg acgagccgcc cgtcaggctc
     7681 gagcgcgacc gcggcgattg tcggcgttga gtagatgcgg aatggcagct ccgtcccgag
     7741 cctggcaata ataaacggta gactcgtggt gccggcgaac aggcacaccg acaagatcgc
     7801 gcttaccgca aatagccgta cggccgccag catagggtcc cctttggggg acccttgccc
     7861 gaatccgtgg gcggcgagac gcctgcacta cgactgaacc cggcaccaag caaaattccc
     7921 ccggcctcga cctagctcgg cctctatgcg tcgcaggccc actccgtggg cgatattagt
     7981 ggtcgttatc cctggcgggt ggcgatgagt cggaattcca ggcgtgcgtc gttctccgtt
     8041 ttcaggaaga gaatcgccgg cggatcgaaa ccgaagtcgg tcatcttgat ggtcgtgtta
     8101 ccagtggctg tcagtcggtc gccgtcgagc ctgaccgttc ccgtccaggt caccggccgg
     8161 gtgatgtctc gcaccttgag gttcccgctg aactccactg ggatctcgcg accatcctga
     8221 tacgttgatg gtagcccttt gatcgaagtc accgtgaact gcgccatggg aaactttgca
     8281 gattccagcc accgctcttt gatcgcgttg tcacggcggt cactgtccga tttgaactgg
     8341 ctgatgtcga cggtaaccgg gccgatgcgg ctctcggcgg gccgcgcgcg attgacgatg
     8401 acctcgccgc gcacagcgct cgtggtcccg accgctgttt gaatcctgtt gccttctcta
     8461 aacagggttt cgtctacgcg ataggtcacc gtggactggc tcgggacgat cacgaagcgc
     8521 tgcgtgcccg acggggccgc cgtcgacgca cccgttggag ctgatacctg attctgcggg
     8581 tttggcggag tcgaaacgcg ctcgggtgcc gctgtagaga cgaggtaggc tagggtcagg
     8641 ccggcgccca ggactgcgag aaggatcaag agagatctgt tcatgcttct ctcttttccc
     8701 aggtgcaaca gggacggtgg ggccggagac cgaccccacc atgatgttac ggacatgtta
     8761 ccgtgttccg atctcttaac gccgcctcat caacagctcg gcgcagcgga acgcctgttc
     8821 cctgttttga acggggatta ccgagcgcag atcccacagc agatccagcc gtgcgcgcgc
     8881 gaatgcattg cgcgcgtcat tgctcttcgc aatctgagca tcaaccgcgc cgcgcgtcgt
     8941 tgtatcggcg accaggagcg tgcgaagggc ccgctcttct cgccccatgc tttcgcgcgc
     9001 cgtctgcagg cggtcgcgat gccgagcaag aacggggttc actgcatcct gtgagatgac
     9061 tgactggcac tgctgcgccc tgcgtgcccc gggcggcgga ccctgttggg cgagcgccga
     9121 ggagatcatc agaagcccgg ccagtacagc cacaaccaag cctgacgcga gcaatacctt
     9181 gaacctgaac atgaatgttg ccaccccttt cacgatgtca ccctgacaga cggagcgtcc
     9241 ccttcgtgcg tttagttatc tggagagtca acgatcgtgg acgccccaac gtcaatgcgg
     9301 atggagtaaa agtgaggagg tcaatgatga agatcatcgc cctgagcttt tgcgcgctgc
     9361 tcatttggaa cgcgaccggc acggccgccc cgagacaggt gatgcctcca ccacctgcct
     9421 gcacgccaaa cgcaccggtg actccgtctc agaccgaagg acccttctac aaagcaaaca
     9481 ctccagagcg tacgtc
//
LOCUS       DBNGSB010000167         4881 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_118574, whole genome shotgun sequence.
ACCESSION   DBNGSB010000167 DBNGSB010000000
VERSION     DBNGSB010000167.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 4881)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4881)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4881
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_118574"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..467
                     /gene="rnpM"
                     /locus_tag="AC159B_04845"
                     /gene_synonym="ylxR"
     CDS             204..467
                     /gene="rnpM"
                     /locus_tag="AC159B_04845"
                     /gene_synonym="ylxR"
                     /inference="COORDINATES: protein motif:HMM:NF047356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase P modulator RnpM"
                     /protein_id="HIR3377106.1"
                     /translation="MRKVPTRTCVACRRARPKREMVRVVRTAAGEVRVDPTGKLSGRG
                     AYVCPEPECVDVGVREQRLQYALEVQIPEDVVQALRGLTHASA"
     gene            454..2874
                     /gene="infB"
                     /locus_tag="AC159B_04850"
     CDS             454..2874
                     /gene="infB"
                     /locus_tag="AC159B_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIR3377107.1"
                     /translation="MRVHELAKDLGLSSKALMDLLAKMKIPARSHSSSLDEAAIARIR
                     QQLKAPPKPAAPVAPARIAKTPTGERILGMRKIVRPVVEEAPIATAPAAEPLVIEPPP
                     VAPPANLPEPVKPKPVVVPVEGKKTVPKPAPEAPAIKPAARAPALPKAPQVDTRLPGT
                     RTPRPMLRPVPRKEAPRAVPPRGPAEAPAASSGAPAAAAPTARERKTRERLPSPPPPP
                     LPAAPVVPTEVELYSALTVGDLATKLELPGGEIVKRLLEQGVLAGINQQISREAASKV
                     AESFGSSVKKPAAAGQPGSRASKRLEVSKEGTVAQRPPIVTIMGHVDHGKTTLLDAIR
                     QTDVAGREFGGITQHIGASTVDIDGRRIVFIDTPGHEAFTALRARGAQVTDIAVLLVA
                     ADDGVMPQTVEALNHARAAGVPVMVVINKVDLPQANPDRVKQQLSELGLVPEDWGGDT
                     VTVPVSARTKQGVKELLEMILLVADLQELRAPADRPARGTIVEAELDKGRGPVATVLV
                     QEGTLRVGDAIVAGESYGRVRAMTGDRGERLNEAGPAAPVEVVGLDAMPQAGDLLEVL
                     RDDKLAKAVAEERRERRRASEVAVARPSMGAEEAGEAGPKEARLIIKADTHGSVEALT
                     SSLSRLAIPTVGVTILHAAVGNVAESDVMLAAASRAAIIGFNVRPEPQVRKLAEQEQI
                     EILTYRVIYEAIEDLQKRLRGMLAPKRREVALGQAEVRQTFTISRIGTIAGSFVAAGT
                     IPRSANVRLIRDGAVVHEGKISSLRRFKDDVREVTEGFECGIGLERFNDIKVGDIIEA
                     YEIREEPA"
     gene            2968..3249
                     /locus_tag="AC159B_04855"
     CDS             2968..3249
                     /locus_tag="AC159B_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HIR3377108.1"
                     /translation="MVVGVLHIQLSLPGAGSLKDKRRLLTSLLDRLHNRFNVAAAEVD
                     QQDVWRRADVAVACVSSEGRHANQILTRVMAEVERANELVVVAFEMELR"
     gene            3257..3655
                     /gene="rbfA"
                     /locus_tag="AC159B_04860"
     CDS             3257..3655
                     /gene="rbfA"
                     /locus_tag="AC159B_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HIR3377109.1"
                     /translation="MTRRTEKLQEFIKEQVSEIIQQHVRDPRIGFVSITDVEVSADMR
                     HAKIFVSVLGDDDAKSGTMAGLQSAVKFIRGELGRRLEMRYTPEIMFKLDTSIERGSR
                     VVSLIRDVTQGVSHDDDSAPTDRDDASDKS"
     gene            3603..4571
                     /locus_tag="AC159B_04865"
     CDS             3603..4571
                     /locus_tag="AC159B_04865"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013528.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIR3377110.1"
                     /translation="MTTTLPRQIATTLRTSPDVLIACHVAPDGDCIGASLALALATSR
                     LGVRSVVGSADGVPDAFLDLPGADRIISTPPVETFATAVAMECSTIERAGVFADTLRR
                     AGTLINIDHHLSNAGFGHVVYWEKGAAAVGELITEIIRALGTPIDAGIAQCLLAAIVT
                     DTGVFRFTNVTPHTLRLAAELLDAGGSIHRVVERVYETRSPEGLRLLGMALAAVRLSA
                     DGRVAWTTVTPEMLRACGARPEDTTGIVGMLRQIRGVKVALMFESTADGVRASIRSRD
                     GARANVIAETFGGGGHQAAAGFTLAGSMNDVVGKTLLEVEKELRAE"
     gene            4597..>4881
                     /locus_tag="AC159B_04870"
     CDS             4597..>4881
                     /locus_tag="AC159B_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HIR3377111.1"
                     /translation="MIRLCKPVGMTSHDVVDAVRRIVGTRRVGHTGTLDPGAAGVLVL
                     VVGQATRVAEFIADADKEYRVEIVFGRSTDTGDAYGAVVRESTAGRVGPDA"
BASE COUNT          937 a         1489 c         1658 g          797 t
ORIGIN      
        1 tacagcggcg gctcccgaag gagctggtgc tgtggagaga ccgccggtgg acgctgttcc
       61 tggcgaagcg cccgaggccg ctgcgtcaga cggagaagcc gaggctgctg tgtcagacag
      121 ggaagccgaa accgctgcgt cagacggaga tgtacttgag acgtctgtga aaaaggaacc
      181 ctcgacggcg ggcgatgcct agcatgcgaa aggtacccac gcgcacgtgt gtggcatgtc
      241 gtcgcgcgcg tccaaagcgt gagatggtgc gagtggtgcg taccgctgcc ggtgaagtca
      301 gagtggatcc gaccggaaag ctgtcgggga gaggcgcgta cgtctgtccc gagccggaat
      361 gcgtggacgt cggcgtgcga gagcagcgcc tccagtatgc cttggaagtt cagatcccag
      421 aagacgtcgt gcaggcatta cgcgggttaa cacatgcgag tgcatgaact agcaaaagat
      481 ctgggcctat ccagcaaagc gctgatggat ctcctggcca aaatgaaaat accggccagg
      541 agtcacagca gctctctgga tgaggccgcc atcgcccgca tccgtcaaca actcaaagcg
      601 ccccccaagc cggccgcccc tgtcgcaccg gcgcggatcg caaagacacc gaccggcgag
      661 cggatcctcg gtatgcgaaa gatcgtgcgt ccggtagtgg aggaggcgcc catcgcaact
      721 gccccggccg ccgagccgct ggtgatcgaa cctcctccgg tcgcgccccc agccaatctg
      781 cctgagcccg tcaaaccgaa accggtcgtc gtcccagtag agggaaagaa gaccgtacca
      841 aaaccggcgc cggaagctcc cgcgatcaag cccgcggcca gggcgcctgc tctgccgaag
      901 gctccgcagg tggatactag actccctggc acacggacgc cgcggccgat gctgcggccc
      961 gttccgcgta aagaagcgcc gagagcagtg cctccgcgcg gccccgctga ggctcccgca
     1021 gcgtcgagcg gcgcgcctgc ggctgcggcg ccaaccgcaa gggagcggaa gacaagggaa
     1081 cggttgccgt ctccgccgcc accaccactt cctgctgcgc cggtagttcc taccgaggtc
     1141 gaactctaca gcgcgttgac tgtgggcgac ctggccacga agctcgagct gcccggcggt
     1201 gaaatagtca agcgactcct cgagcagggt gtgctggcag gcatcaacca gcaaatctcg
     1261 cgcgaggcgg cgtccaaagt cgcggaatcg ttcggctcat cggtgaaaaa gccagcggcc
     1321 gcgggtcagc cgggaagccg ggcgtcgaag cgcctcgagg tttcgaagga aggaaccgtt
     1381 gcgcaacgac cgcccatcgt caccatcatg ggtcacgtcg accacgggaa gacgacattg
     1441 ttggacgcga tccggcagac cgatgtcgca ggcagagagt tcggggggat cacgcagcac
     1501 atcggcgcgt cgacggttga tatcgacggt cgccgtattg tcttcatcga cacaccggga
     1561 cacgaagcgt tcacggctct gcgggcgcgc ggagcccagg tcaccgatat tgcggtcctg
     1621 ctcgtcgcag cggacgacgg cgtcatgccg cagacagttg aggcactcaa ccacgccaga
     1681 gccgcgggcg tcccggtcat ggtcgtcatc aacaaggtag atctgcccca agccaatccc
     1741 gatcgcgtga agcagcagct ttcagagctt ggtctggtcc ctgaagactg gggcggcgat
     1801 acggtcaccg ttcccgtctc tgcgcggacg aagcaggggg tcaaggaact cctcgaaatg
     1861 atcctgctgg tcgcagacct gcaggagctg cgtgctcccg cggaccgccc ggctcgtgga
     1921 accatcgtcg aagctgagct cgacaaaggc cgcggtcccg tggcgactgt gctcgtgcag
     1981 gaagggacgc tcagagtcgg cgatgccatc gtggccggcg aatcgtatgg ccgtgtccgg
     2041 gcgatgacgg gtgatcgcgg cgagcgcctg aatgaagccg ggcccgcggc gcccgtggaa
     2101 gtcgtggggc tcgatgccat gccgcaggcc ggcgatttgc tggaggtcct gcgcgacgac
     2161 aagctcgcaa aggctgttgc ggaagagcgc cgcgagcgac ggcgtgcatc ggaggtcgcg
     2221 gtcgcgcgcc cgtctatggg cgcagaagaa gccggcgagg cgggaccgaa ggaggcgcgt
     2281 ctgatcatca aggcagacac gcatggatcc gtggaggcgt tgacgtcctc tctatctcga
     2341 ctggcgattc ccacagttgg ggtgacgatt cttcacgcgg cggtcggcaa cgtggcggag
     2401 tctgatgtga tgctcgccgc agcgagccgc gcggccatca tcggcttcaa cgtccgtccc
     2461 gagcctcagg ttcgcaagct ggccgaacag gagcagattg agatcctcac ttaccgggtg
     2521 atctacgaag cgattgagga tctgcagaag cggctgcgcg gcatgctggc gccgaagcgg
     2581 cgcgaggttg cgctcggcca ggccgaggtc cgccagacct ttacgatctc gagaatcggg
     2641 acgatcgccg gctcatttgt tgcggctgga accatccccc gcagcgcgaa tgtccgtctc
     2701 atccgggacg gtgcggtcgt gcacgagggc aagatctcgt cgctgcgccg cttcaaggat
     2761 gatgtccgcg aagtcaccga aggcttcgaa tgcggcatcg gactggaacg attcaatgac
     2821 atcaaagttg gcgatatcat agaggcgtac gagataaggg aagagcctgc gtaaatgacg
     2881 gaaaccggga aacggggaaa cggggaaacg cgaaactaag atcgtcgcga ttccgcgatt
     2941 ccacgtttcc gcgcttccgt ggttgccatg gtcgtcggcg ttctccacat ccaactcagt
     3001 ctaccgggcg caggcagttt gaaggacaag cgccggctgc tcacgagtct gctcgaccgg
     3061 ctccacaacc ggttcaatgt cgcggcggcg gaagtcgatc agcaggacgt ctggcgccgg
     3121 gccgacgtcg ccgtcgcctg tgtcagcagc gaaggccggc acgccaacca gatcctcacg
     3181 cgcgtgatgg cggaagtcga gcgcgcgaac gaactcgtcg tggtggcgtt cgaaatggaa
     3241 ctacggtagc agtgggatga cgcgacgaac cgaaaaactg caggagttca tcaaagagca
     3301 ggtgagcgag atcatccagc agcatgtccg ggatccccgc atcgggttcg tctcaatcac
     3361 cgatgtggag gtcagcgcag atatgcgcca tgcgaagatc tttgtcagcg tgctggggga
     3421 tgatgacgcg aaatcgggga cgatggccgg cctgcagagc gctgtgaagt ttatccgcgg
     3481 cgagctgggc cgcaggctgg agatgcggta cacgccggag atcatgttca agctagatac
     3541 gtcgatcgaa cgcggttcac gggtcgtgtc attgatccgg gatgtgaccc agggagtctc
     3601 gcatgacgac gactctgccc cgacagatcg cgacgacgct tcggacaagt cctgacgttc
     3661 tcatcgcctg ccacgtcgcc ccggacggag actgcatcgg cgcgtcatta gccctggcgc
     3721 ttgcgaccag ccgccttggg gtgcgctccg tggtgggcag cgccgacggc gtgcccgatg
     3781 cgttcttgga cctccccggc gcggatcgga tcatcagcac accccccgtc gagacgttcg
     3841 ccactgcggt ggcaatggag tgcagcacga tcgagcgcgc gggtgtattc gcggacaccc
     3901 tgcggcgtgc cggtacgctc atcaacatcg accaccatct cagcaatgcc ggattcggcc
     3961 acgtcgtgta ctgggagaag ggcgcggccg cggtcggcga gttgattacc gaaatcatcc
     4021 gggcgctggg gactccgatc gacgccggca tcgcgcagtg cctgctggcg gccatcgtca
     4081 cagatactgg agtcttccgg ttcacaaacg tgacaccaca taccctccgt ctcgccgcag
     4141 agctcctcga cgccggcggc tccattcacc gggtggtgga gcgtgtctac gaaacccgat
     4201 cgccggaagg gctgcgattg ctcggcatgg cgctggcggc cgtgcgcctt tctgcggacg
     4261 gccgtgtcgc ttggacgacg gtgacaccgg aaatgctgag ggcgtgcggc gcgcggccgg
     4321 aggataccac cgggatcgtc ggcatgttgc ggcagatccg cggcgtgaag gtcgcgttga
     4381 tgttcgagtc gacggccgac ggagtgaggg caagtatccg ctcgcgagac ggcgcccgcg
     4441 ccaatgtgat cgctgagact ttcgggggcg gcggccatca ggccgcggcg gggttcacgc
     4501 tggcaggctc catgaatgac gtcgtcggca aaactttgct ggaagtcgaa aaagaactgc
     4561 gtgccgagta accccccatc gccgtccgcc ggcggcgtga ttcgtctctg caagccggtt
     4621 gggatgacct cccatgacgt cgtcgatgct gtgcgccgca tcgtgggtac gcgcagggtc
     4681 ggccacacag gcacgcttga cccgggcgcg gccggtgtgc tcgtcctcgt cgtgggtcag
     4741 gccacgcgcg tagccgagtt catcgccgat gccgacaagg aatatcgggt cgagatcgtg
     4801 ttcgggcgtt caaccgacac cggcgacgcg tatggggcgg tcgtccggga aagcaccgcg
     4861 ggtcgcgtag gacccgatgc a
//
LOCUS       DBNGSB010000168         5125 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2573151, whole genome shotgun sequence.
ACCESSION   DBNGSB010000168 DBNGSB010000000
VERSION     DBNGSB010000168.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 5125)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5125)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5125
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2573151"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..608
                     /locus_tag="AC159B_04875"
     CDS             120..608
                     /locus_tag="AC159B_04875"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015342513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR3377112.1"
                     /translation="MQEKTVSMTVLMTPNKANFAGNVHGGELLHLLDQVAYVCASRYS
                     KSYVVTLSVDQVFFKEPIHVGELVTFLASINFVGKSSMEVGIRVEAEDIRTKKKRHTN
                     SCYFTMVAVDETGRPVEVALLVPEGPIEQRRFEDAKQRREIRKSIASGSLSRANQPAF
                     GK"
     gene            631..1029
                     /locus_tag="AC159B_04880"
     CDS             631..1029
                     /locus_tag="AC159B_04880"
                     /inference="COORDINATES: protein motif:HMM:NF024996.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HIR3377113.1"
                     /translation="MIVAFAVCTTALAQGTNVTLYGTLNVDFERVEAKDTSRSGPGNN
                     FVGAPSALSPELDARNRVSSNASNIGLRGTEELGNGLKAIFQCESTAAIDGGTAGLCT
                     RNSHVCLSGAWGTSTPTRCTTRNYLTLVTR"
     gene            1026..1253
                     /locus_tag="AC159B_04885"
     CDS             1026..1253
                     /locus_tag="AC159B_04885"
                     /inference="COORDINATES: protein motif:HMM:NF019511.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1674 domain-containing protein"
                     /protein_id="HIR3377114.1"
                     /translation="MRPPADYGDDTESSPARSRQLTEPAAGQAQPLAEIPVATSGSSA
                     SDPKPREIGGPKGPEPTRYGDWEKGGRCIDF"
     gene            1303..1680
                     /gene="sdhC"
                     /locus_tag="AC159B_04890"
     CDS             1303..1680
                     /gene="sdhC"
                     /locus_tag="AC159B_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HIR3377115.1"
                     /translation="MISDKRPLSPHLQVYRLPFIVMMSITHRMTGVGLVAGALLLVYW
                     LVAAANGPAAYADAQRLLGSLVGKLFLFLWTWALFYHMANGIRHLAWDLGYGFELESA
                     DRSGMSVVIVSVVLTILTWLIAI"
     gene            1694..2074
                     /gene="sdhD"
                     /locus_tag="AC159B_04895"
     CDS             1694..2074
                     /gene="sdhD"
                     /locus_tag="AC159B_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HIR3377116.1"
                     /translation="MRFRTPLSHVRGLGSAKEGTHHWWLERTTSAALIPLTLWFIISL
                     VFISRYDHAELAAWIARPFNTALLVLFLTLTFHHSILGIQVVIEDYVHHEGYRLAALV
                     ATKFLLVLCGVIGVIAVLRIAFGG"
     gene            2079..3869
                     /gene="sdhA"
                     /locus_tag="AC159B_04900"
     CDS             2079..3869
                     /gene="sdhA"
                     /locus_tag="AC159B_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HIR3377117.1"
                     /translation="MPNAYKIINHDYDVVVVGAGGAGLRATFGMAAKGLSTACITKVF
                     PTRSHTVAAQGGISAALANMGPDDWRWHMYDTVKGADWLGDQDAIEYMCKHAMTAVIE
                     LEHQGVPFSRTEDGRIYQRPFGGMTTNYGEGQAYRTCAAADRTGHAILHALYQQSLKY
                     QAEFFIEYFAIDLIMDESGVCRGVVAWDLGDGTLHVFRAHMTVLATGGYGRAFFSATS
                     AHTCTGDGNALVLRAGLPLEDMEFTQFHPSGIYGSGCLVTEGARGEGGYITNSLGERF
                     MERYAPNAKDLASRDVVSRAMTIEIREGRGVGELKDHIHLHLEHLGPKVLQERLPGIC
                     ETARIFAGVDATKEPIPVLPTVHYNMGGIPTNYFGEALTLKDGNPDSLVPGLMAIGEA
                     ACVSVHGANRLGSNSLLDIIVFGRAAAERCGELVRPRQTHASLPQRAVDKIIEHFDRL
                     RHANGSQPTAAIRLEMQRTMQTHAAVFRTEQSMAEGIRRLTDIFHSFADVKVADRSLI
                     WNSDLVETLELENLLGCAMVSIHSAINRKESRGGHAREDYPERDDENWMKHTLAWMDS
                     DGKVTIGYRPVHTYTLTDEIEYIPPKARVY"
     gene            3883..4659
                     /locus_tag="AC159B_04905"
     CDS             3883..4659
                     /locus_tag="AC159B_04905"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (quinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HIR3377118.1"
                     /translation="MAEFRLPVNSRMRKGKVHVNPEGKRPKRFEIYRWDPDSGENPRA
                     DTYTVDLAACGPMILDALIKIKNEIDATLTFRRSCREGICGSCAMNIDGRNTLACTKP
                     IDEVKGDAKIYPLPHMPVVKDLVPDLTHFYAQYASIKPWIQTQTPPPPDRERLQSKED
                     RAKLDGLYECILCACCSTSCPSYWWNGERYLGPAVLLQAYRWIADSRDEFTGERLDDL
                     EDPFRLYRCHTIMNCAKVCPKGLNPARAIGEIKKLMVERR"
     gene            4659..4985
                     /locus_tag="AC159B_04910"
     CDS             4659..4985
                     /locus_tag="AC159B_04910"
                     /inference="COORDINATES: protein motif:HMM:NF015870.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006105 - succinate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0034552 - respiratory chain complex II
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HIR3377119.1"
                     /translation="MSLGVAKLRWRCRRGMKELDLLLLSYLERRYDTASEEERWIFSE
                     LLELPDPKLHAYLLGREQPAVHAIADVVRAIRDAVDRRTRAGWLSPSMLSMKLSTGFA
                     EDRRTP"
BASE COUNT         1047 a         1506 c         1559 g         1013 t
ORIGIN      
        1 ggtgattctt ggctataccg cgtattccta ttgggtgttc cgcggcaaga tcacgcgcga
       61 tgcggggtat catcactgat tgcaaccgac ggattcaact cgggctgtta aggacatcaa
      121 tgcaagagaa gaccgtatcg atgacggtgc tgatgacccc aaacaaagcg aacttcgcag
      181 ggaacgtgca cggtggggag ctgctgcatc ttctcgatca ggttgcgtac gtatgcgcgt
      241 cacgatacag caaatcctac gtcgtgacct tgtctgtcga tcaagtcttc ttcaaggagc
      301 caatccacgt cggcgagttg gtgacctttc ttgcgtctat taacttcgtc ggcaagagtt
      361 caatggaggt cggtatcagg gttgaggcgg aggacatcag aacgaaaaag aagaggcaca
      421 cgaactcctg ctatttcacc atggtggcgg ttgacgaaac gggaaggcca gtcgaggtcg
      481 ccttgctcgt cccggaagga ccgatagagc aacggcgctt cgaagatgcg aaacaaaggc
      541 gcgagattcg aaagtccatc gccagtggaa gcttatctcg cgcgaaccag cctgcattcg
      601 gcaaatgaaa gtcagcggat cgcataccga gtgattgtcg ccttcgccgt ttgcacaacc
      661 gcgctcgctc aagggacgaa cgtgaccctc tacggcacac tgaacgtgga tttcgagcgg
      721 gtagaggcga aagataccag cagaagcggc cccggtaata acttcgtcgg cgcgccttcc
      781 gcgctctctc ccgaactgga tgctcgcaac cgcgtttcgt caaatgcctc caacatcgga
      841 ttgcgaggaa ccgaggagct cggcaacggc ctcaaggcga tttttcagtg cgaatccacg
      901 gccgccatcg atggaggtac cgccgggctc tgtactcgca acagccatgt ctgcttgagc
      961 ggcgcttggg gcacttccac acccacaaga tgcacaacac ggaactacct gactttggta
     1021 acgagatgag acctccagca gactatggcg atgacaccga atcttcgccg gcgcgctctc
     1081 gccaactcac tgagccggca gccgggcagg cccaaccact cgctgaaatc cccgtggcta
     1141 cgagtggcag ttcggcaagc gatcccaagc cacgggaaat cggcggtccc aaagggcccg
     1201 agccgacccg atacggcgac tgggaaaaag gcgggcgctg cattgatttt tgagcgtcca
     1261 aaaaacgcgt tttcgacgca tcaacataag aggtgaacag cgatgatttc ggacaagcgg
     1321 ccgttgtcgc cgcatcttca ggtttacaga ctaccgttca tcgtgatgat gtcgatcacc
     1381 caccgcatga ccggggtggg tctggtcgcc ggtgcgctgc tgctggtgta ttggctggtc
     1441 gccgcggcca acggaccggc ggcctacgcc gatgcccagc ggttgctggg ctcgctggtg
     1501 gggaagctgt tcctgttttt atggacgtgg gcattgttct atcacatggc gaatggcatc
     1561 cgccacctgg cctgggatct cggctatggc tttgagctcg agagcgccga tcgcagcggc
     1621 atgtcggtcg tgatcgtttc ggtggtcctg actattctga cctggctgat cgccatctga
     1681 agggggaaat agtatgaggt ttcgcacacc gcttagtcat gtccggggcc tcggctccgc
     1741 caaggaggga acccatcact ggtggcttga gcggaccact tcggccgcgc tgatcccgct
     1801 gacgttgtgg ttcattatct cgctggtctt catctcccgc tatgatcatg ccgagctcgc
     1861 ggcgtggatc gcgcgcccgt tcaatactgc gctgctggtg ctgtttctga ccctcacttt
     1921 ccaccattcc atcctgggaa ttcaggtggt catcgaggac tacgtgcatc acgagggcta
     1981 tcggctggcc gcgctggtgg cgacgaagtt cctgctggtg ttgtgcggtg taatcggcgt
     2041 tatcgctgtt ctgcgtatcg ccttcggagg ctagcgccat gcctaacgca tacaagatca
     2101 tcaatcatga ttacgatgtg gtcgtcgtag gagcgggtgg agccggcttg cgcgcgactt
     2161 tcgggatggc cgccaaggga ctctctacgg cctgcatcac caaggtgttt ccaactcgca
     2221 gccataccgt tgccgcccag ggaggcatat cggcggcgct tgccaacatg gggccggacg
     2281 actggcgctg gcatatgtac gacacggtca aaggcgccga ttggctgggc gatcaggacg
     2341 cgatcgagta catgtgcaaa catgcgatga ccgccgtgat cgagctggaa catcaaggtg
     2401 tcccgttttc ccggaccgag gacgggcgca tctaccagcg ccccttcggt ggaatgacca
     2461 ccaactacgg cgagggccag gcttaccgca cttgcgccgc cgccgatcgc accggccatg
     2521 ccattctgca cgctctctac cagcagtccc ttaagtacca ggccgagttc ttcatcgagt
     2581 atttcgcgat cgacctgatc atggacgaaa gcggcgtctg ccgcggcgtg gtggcctggg
     2641 atctcggcga cggcactctg cacgtattcc gcgcccacat gacggtgctc gcgaccggcg
     2701 gttacgggcg agccttcttc tcggcgacca gcgcccatac ctgcaccggc gacgggaacg
     2761 ccctggtttt gcgggcgggg ctgccgctgg aggacatgga attcacccag tttcatccga
     2821 gcggcatata tggctctggc tgtctggtga ccgaaggcgc gcgcggcgag ggtggctata
     2881 tcaccaactc cctcggcgag cgcttcatgg aacgctacgc ccccaacgct aaggatttgg
     2941 cctcccgcga cgtcgtgagc cgcgctatga cgatcgagat ccgtgagggc cgcggcgtgg
     3001 gcgagcttaa ggatcacatc catcttcacc tcgagcatct gggcccgaaa gtgctgcagg
     3061 aacgcctgcc cggtatttgc gagaccgcca ggatcttcgc tggcgtggac gcaaccaagg
     3121 agccgatccc ggtgctgccg acggtgcatt acaacatggg tggcatcccg acaaactact
     3181 tcggcgaagc gctgacgttg aaggatggca atcccgattc tctggtaccc ggcctgatgg
     3241 cgatcggcga agctgcctgc gtctcggtgc acggggcgaa ccgcctgggc tccaattcgc
     3301 tgctggacat aatcgtgttc ggccgggctg cggccgagcg ttgcggcgag ttggttcgcc
     3361 cccgccagac gcatgcgtcg ctaccgcaaa gggcggtgga taaaatcatc gagcacttcg
     3421 accgtttgcg tcatgccaac ggttcccaac cgaccgccgc gatccggctc gaaatgcagc
     3481 gcacgatgca aacccacgcc gctgtgttcc gcaccgagca gtccatggca gaagggatcc
     3541 ggcgcttgac cgatatcttc cattcgtttg ccgatgtgaa ggtcgccgac cgcagcctga
     3601 tctggaactc ggacctcgtg gagacgctgg agcttgagaa cctgctgggt tgcgcgatgg
     3661 tgtccattca ctcggcgatc aaccgcaagg aaagccgggg cggccacgcc cgcgaggact
     3721 acccggagcg ggacgacgaa aattggatga agcacacgct cgcgtggatg gactcggacg
     3781 gtaaggtcac cattggttac cgcccggtcc atacctacac gctcacggac gagatcgagt
     3841 acattccgcc caaggcgcgc gtttactaaa aggagtgaga cgatggctga attcaggctt
     3901 cctgtaaatt caaggatgcg aaagggcaag gtgcacgtaa acccagaggg gaagcggccc
     3961 aaacgtttcg agatctaccg ctgggatccg gatagcggcg agaatcctcg ggccgatacg
     4021 tacactgtgg acctcgccgc ctgcggtccg atgattttgg acgcgttgat caagatcaag
     4081 aacgagatcg atgcgacatt gaccttccgc cgctcctgcc gcgaaggaat ctgcgggtcc
     4141 tgcgcgatga acatcgacgg tcgcaatact ctcgcctgca ctaagccgat cgatgaggta
     4201 aaaggggacg cgaagattta cccgctgccc cacatgccgg tcgtcaagga cctcgtaccg
     4261 gatctcaccc atttttatgc gcagtacgct tcgatcaaac cctggatcca aactcagacc
     4321 ccgcctccgc cagaccgcga acggctgcaa tcgaaggagg atcgggccaa gctggacggg
     4381 ctctacgagt gcatcctgtg cgcctgctgc tcgacgagtt gcccgagcta ctggtggaac
     4441 ggtgaacgct atctaggtcc cgcggtcctg ctgcaagcct atcgctggat cgcggacagc
     4501 cgggacgagt tcaccggcga gcggctggat gacctggagg acccgttccg gttgtatcgc
     4561 tgtcacacga tcatgaactg cgccaaagtc tgtccaaaag ggctcaaccc ggccagggcc
     4621 attggcgaaa tcaagaagct aatggtggag cggcgttagt gagtttaggc gttgcaaaac
     4681 tgcgctggcg ctgccggcgc ggtatgaagg agctggacct gctgctgctg agctatctgg
     4741 agcggcgcta cgacacggct tcggaagagg agcgctggat attctcggaa ttgctggagc
     4801 tgccagatcc taagctgcac gcctatctgc tcggccggga acagcctgcg gttcacgcga
     4861 ttgccgatgt tgtccgcgcc atccgggacg ctgtggatcg tcgaactcgg gcaggatggt
     4921 taagcccttc gatgctttcg atgaagctca gcacaggctt cgctgaggac aggcgaaccc
     4981 cttgattgga tcggtcaagg acttcgttct cgcagcgagc gagacgccgc cccaaccagc
     5041 gaccctggac ccggacgcgg accctcctcc gatttagtgc atgatcatct tcaacgggag
     5101 aaaccgagat gaaaaagaaa ctcct
//
LOCUS       DBNGSB010000169         5343 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_4106697, whole genome shotgun sequence.
ACCESSION   DBNGSB010000169 DBNGSB010000000
VERSION     DBNGSB010000169.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 5343)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5343)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5343
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_4106697"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..2005
                     /locus_tag="AC159B_04915"
     CDS             299..2005
                     /locus_tag="AC159B_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIR3377120.1"
                     /translation="MTALPPPSPSPRLARWLPALGWLRSYDSGWLRPDLIAGITLAAY
                     LMPAAVGDASLANLPPEAGLYACLLSGLIFWLFCSSRHTAITVTSAISLLIGASLGGL
                     ADGDTTRFGALAACTALLVAALALLAWFFKAGSLVNFVSETVLVGFKAGVACYLTSTQ
                     LPKLFGIKGISGDFWERMGDFFRHLDETNSAALAVGLGALAVLVLGKVLWKNKPVALF
                     VVVGGIMVASLADLDARGVKLLGEVPQGLPPLGLPAVQWSDLNELLPLALACFLLGTV
                     ETAAIGRMFAAKHGGRFDANQEFLALAGANLAAGLGQGFPVSGGMSQSLVNESGGART
                     PLSSLVAAGIILLVVIFLSGLLRHLPQPVLAAVVLVAVAGLVQLSALQWLWRFHRGEF
                     LVAMAALLGVLGSGLLRGVLIGAIISLIQLIQRASHPHVAFLGRIPGTRRFSDRARNP
                     DNESIAHVLVFRPESSLVYFNVDHVCDTVRDRVRAESPPPRYVVCDLSASPRMDLQSA
                     HTLAGLVADLAAVGIRTEVVEARAAVRDLLRIEGLDESLGGIDHFTSVADAVEGFQKE
                     GK"
     gene            2092..2244
                     /locus_tag="AC159B_04920"
     CDS             2092..2244
                     /locus_tag="AC159B_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3377121.1"
                     /translation="MRLTTLGLALIVVFWHVGLGQFPGRRIPEGGCPGVLIGAMVSGD
                     RSIQLV"
     gene            2345..3799
                     /gene="sthA"
                     /locus_tag="AC159B_04925"
     CDS             2345..3799
                     /gene="sthA"
                     /locus_tag="AC159B_04925"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010041710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HIR3377122.1"
                     /translation="MPINSSLQPGSAETRAFALVVLGGGPAGTCGAGTAAVFGRRVAL
                     VEKEPEIGGAGINSGTVPSKTLRETALALSGWRSRKLHGVDLSLRREATVADFTRHQR
                     QVSTGERRRIETRLDALSVERFHGIASFVDPHTVGVARRDGSKLRLSGEKILIATGSS
                     PFHPPEFLFEDDRIHDSDELLQIRALPRRLAVIGAGVIGSEYACTFAAMGVEVHLVDG
                     RDALLPFLDGEVSRALAAAMAAGGVRFHWRERVTQCDASQPGDVRLTLSSGTEFDCDG
                     VLVCSGRSSNTSELNLVAAGITPGKRGVIPVDAHYCTEVGHIYAAGDVIGAPALAATG
                     MEQARVAMCHAFGITAKADLAPLRPAGIYTIPEVSTIGQTEESLKQQGVDYIAGRARY
                     ADNPRGRIIGDDTGFLKLLFRRDDMRLLGVHVIGEQATEVVHPGLVALLTESGADLFN
                     RACFNYPTLGDLYKYATYDALLQRGRLSEAPNNA"
     gene            3971..4096
                     /locus_tag="AC159B_04930"
     CDS             3971..4096
                     /locus_tag="AC159B_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3377123.1"
                     /translation="MIRLWRVNGEQLTLATFSQPVALLGLGGATGGFLIDGSDRR"
     gene            4464..>5343
                     /locus_tag="AC159B_04935"
     CDS             4464..>5343
                     /locus_tag="AC159B_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011356226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3141 domain-containing protein"
                     /protein_id="HIR3377124.1"
                     /translation="MTDLNKQLASSYSLSGKVSRVFEKRATLAQEGFTARVRKAFDEN
                     VAELAAKPASPWEIWTDWYSYSVDLAQRSVLFWDTLRQRGNNFVKSTQEGLVPVLHFD
                     YETVLDGRSLERPVNYALVRIVPPEGVTVDPKRRPYVIIDPRAGHGPGIGGFKDDSQV
                     GVALRAGHPVYFVIFFREPQPGQTMLYVCAAEQQFVRKVRSLHPDSAKPAIIGNCQGG
                     WAAMMLAAAGPDDTGPIVINGAPMSYGGGAWQEGESDNPMRYSGGMLGGSWLASFAAD
                     MGNGVFDGAYLVENFES"
BASE COUNT          886 a         1671 c         1736 g         1050 t
ORIGIN      
        1 ttccaggaag ggcgcttacg atgatcaggc ctcgctgaag ggaagaaagt ttgtgccgga
       61 aaagtactga cccgcgttga gccgccactt ctggagaaac ccaggatgag acgatgagga
      121 cgatttaacc caaaaacggc gtttcaacgt aaagacgcga agataaggca agggtacgca
      181 aaggaaaatc ttgggaaccc ttcttaccaa aatgtgatac ccgcttttcc gataaggatt
      241 tgtttttctt tgcgcccttt gcgtctttgc atttgaactg ctttttcaag gctcaacggt
      301 gaccgccctg cccccaccgt cgccttcccc ccggctcgcc agatggctcc ccgcgctcgg
      361 ctggctgcgc tcttacgaca gcggctggct gcgcccggac cttatcgccg ggatcacgct
      421 ggcggcctac ctgatgccgg cggccgtcgg tgacgcctcg ctggccaatc ttccgcccga
      481 ggcggggctt tatgcgtgtc tcttgtcggg actgatcttc tggctcttct gcagctcgcg
      541 gcacacagcc atcaccgtca cctcagccat ctcgctgctg atcggggcct cgctcggcgg
      601 gctggcggat ggcgacacga cacgcttcgg tgcgctggct gcctgcacgg cgttgctcgt
      661 ggcggcgctg gcgcttctcg cgtggttctt caaggcggga tccttggtga acttcgtttc
      721 cgagacggtg ctggttgggt tcaaggcagg cgtggcgtgt tatctcacga gtacgcagtt
      781 gccgaagctc tttgggatca aaggcatctc cggcgatttc tgggagcgca tgggcgattt
      841 cttcaggcac cttgacgaga cgaattctgc cgcgcttgcc gtcggcttgg gtgcgttggc
      901 ggtactcgtg ctcggcaagg tgctttggaa gaacaagccc gtggcgctgt tcgtcgtcgt
      961 cggcggcatc atggtcgcgt cgcttgcgga cctcgacgcg cgcggggtga agttgctggg
     1021 cgaagtgccg cagggccttc ccccgttggg cctgcccgca gtgcaatggt ctgacctgaa
     1081 tgaactcctg ccgctggcgc tggcctgctt tcttctcggt acggtcgaga cggcggccat
     1141 tggacgcatg ttcgcggcca agcacggcgg acgcttcgac gccaaccagg agttcctcgc
     1201 gctggcgggg gcaaacctcg ccgcgggatt gggacagggg ttccccgtca gcggcggcat
     1261 gtcgcagtcg ctcgtgaacg aaagcggcgg cgcacgcacg ccgttgtcca gcctcgtggc
     1321 ggcgggcatt atcctcttgg tggtcatttt tctgtccggc ttgctgcgcc atttgccgca
     1381 gcctgtgctc gcggccgtcg tgctggtggc agtggccggg ctggtccaac tctccgcgct
     1441 gcagtggttg tggcgcttcc accggggtga gttcctcgtg gccatggcgg cgttgctggg
     1501 tgtgctcgga tccggcctgc tgcgcggcgt gctgatcgga gccattatct cgctgatcca
     1561 gttgatccag cgcgcgtcgc atccgcacgt agcgttcctc ggacgcatcc ccggcacccg
     1621 ccggttctcc gaccgcgcgc ggaatccgga caatgaatcc attgcccatg tgctggtctt
     1681 ccggccagaa tcgagcctcg tctatttcaa cgtggaccac gtctgcgaca cggtgcggga
     1741 ccgggtgcgc gccgaaagcc cgccgccccg gtacgtggtg tgcgacctgt ctgcctcccc
     1801 gcgcatggac ctgcaaagcg cgcacacgct ggcggggctg gtcgccgacc tcgcagccgt
     1861 gggcatccgc acggaggtgg tcgaggcccg cgccgccgtg cgcgacctgc tacgaatcga
     1921 ggggcttgac gaatcgctgg gcggcatcga ccacttcacc tccgtggcgg atgcggtgga
     1981 gggtttccag aaggagggaa aatgattgca gcgcagcctt agcacgagag atctcttcag
     2041 ccgaaccttc gtaaactcac tgaacactaa cttgcacttg tcagaggaaa aatgcgattg
     2101 accaccctcg ggctcgcgct tatcgtagtg ttctggcacg tcggcctcgg gcagttcccg
     2161 ggccggcgta ttccggaagg cggatgcccc ggtgtcttga tcggcgcgat ggtctccggc
     2221 gaccggtcaa tccaattggt ataagggcgt ccaggtcatc acggtttacc tgatcatcgc
     2281 cctaatgttc tacttcatgc cggagatctc gcgttagcaa actgtcaagg gggttttgaa
     2341 tgccatgcct ataaattcct cgctccaacc gggctctgcc gaaacccgcg ccttcgccct
     2401 tgtcgtgctt ggcggcgggc cggccggcac ctgcggcgcg ggcaccgccg ccgtgtttgg
     2461 acggcgcgtg gcgctggtgg agaaggagcc ggagattggc ggcgcgggca tcaactccgg
     2521 cacggtgccg agcaagacgc tgcgcgagac cgcgctcgcg ctctctggct ggcgctcgcg
     2581 caagcttcac ggcgtggact tgtcgcttcg tcgcgaggca accgttgccg acttcacgcg
     2641 ccaccagcgg caggtaagca cgggggagcg ccgccgcatc gagacgcggc tggatgctct
     2701 ctcggtcgag cggtttcacg gcatcgcgag cttcgtcgac ccgcatacgg tgggcgtggc
     2761 gcgacgcgac ggctcgaagc tgcggctgag tggcgagaag attctcatcg ccaccgggtc
     2821 ttcgccgttt caccccccgg agtttttgtt tgaagacgac cgtattcacg actcggacga
     2881 gctgctgcaa atccgcgcgc tgccgaggcg gctcgcggtc attggcgccg gcgtgatcgg
     2941 cagcgaatac gcctgcacct tcgcggcgat gggcgtggag gtgcatctgg tggacgggcg
     3001 cgacgcgttg ctgcccttcc ttgacggcga ggtttcacgc gcgcttgccg ccgcgatggc
     3061 agctggcgga gtgcgctttc actggcggga gcgcgtgacg cagtgcgacg cgtcgcagcc
     3121 cggcgacgtg cggctcacgc tctcgagcgg caccgagttt gactgcgacg gcgtgctcgt
     3181 gtgctcaggg cgttcgagca acacctccga gctgaacctc gtcgcggcag gcatcacgcc
     3241 tggaaaacga ggcgtcattc ccgtggacgc gcactactgc accgaggttg gccacatcta
     3301 cgctgccggc gacgtcatcg gcgcgcccgc gctagccgcc actggcatgg agcaggcgcg
     3361 cgtggcgatg tgccacgcct ttggcatcac ggccaaagcg gacctggcgc cgctgcggcc
     3421 cgccggcatc tacaccatcc cggaagtcag cacgatcggg caaaccgaag agtcgctgaa
     3481 gcagcagggc gtggactaca tcgcgggccg cgcccgttac gcggacaacc cgcgcgggcg
     3541 gatcatcggc gacgacaccg gatttctcaa actgctgttc cgccgcgacg atatgcgcct
     3601 gctcggcgtg catgtcatag gcgagcaggc cacggaagtc gttcatcccg gcttggtggc
     3661 gctgctcacg gaatccggcg ccgacctgtt caaccgcgct tgcttcaact acccgacgct
     3721 cggcgacctt tataaatacg ccacctacga tgcgctgtta cagcgcggcc gcctgagcga
     3781 ggcgcccaac aatgcatgaa agcgaatatt ttaatgcaac cctggccaac agactttcca
     3841 cctcgctggt cgggtctcgc tccgctctgc aatgtatggc aggaccactc cattaacctt
     3901 tgcgaaccgc cttccggctc gcgttgagtt gccgaatagg accaaagtcc aattcaccgc
     3961 gcaggatgtc atgattagac tgtggcgcgt gaatggggaa cagttgaccc tcgcgacgtt
     4021 ttcccagcct gtcgcgctgt tgggactcgg cggtgcgaca ggaggtttct tgattgatgg
     4081 ctctgaccgg cgttgacccc tgcgcacccc gaagtgcagg ttatttgatt tagatcaaag
     4141 tgcgagcctg agggtggcgc attattggtc gcgtgcatcg acgtgaatgg gcgcagggat
     4201 ttgtcccaac tggcgcgacc cgaaaccgtt acaaaaagtc gttcttgctt ttgtaatgac
     4261 atgggacgac gatgcactgg actattcttg atcgaaagca agcgaccgtc tgcgccgtgc
     4321 ccccgcggtc tgctgtgcgg agtgttctgc gcggcgccag cgctggcccg gacggcggtc
     4381 caatcaagcg atactatggt ttttgcacaa taagcgtata accggcgaag gccactcgcc
     4441 acgcacctca gaaggagctg accatgacag atctcaataa acaacttgcc agcagttaca
     4501 gcctctctgg aaaagtttcg cgtgttttcg agaagcgcgc gacacttgcc caagaggggt
     4561 tcaccgcgcg ggtgcggaag gcgttcgacg agaacgtcgc cgaactcgcc gcgaagcccg
     4621 cgtcgccgtg ggagatttgg acggactggt atagctattc ggtcgacctt gcgcagcgct
     4681 cggtgctgtt ctgggacacc ttgcgccagc gcggcaacaa tttcgtcaag agcacgcagg
     4741 agggtctcgt cccggtcctg cacttcgact acgaaacggt gctggacggg cgctcgctcg
     4801 agcgccctgt caattacgcg ctggtgcgca tcgtgccgcc ggaaggcgtg acggtcgatc
     4861 cgaagcggcg gccctacgtc atcatcgatc cgcgcgcggg acacgggccc ggcatcggcg
     4921 gcttcaaaga cgactcgcag gtgggcgtgg cgctgcgcgc cggccatccg gtctatttcg
     4981 tcattttctt ccgcgagccg cagccggggc agacgatgct ctacgtgtgc gccgcggaac
     5041 agcaattcgt gcgcaaggtg cgcagcttgc atccggacag cgccaagccc gcgatcatcg
     5101 gcaactgtca gggcggctgg gcggcgatga tgctggccgc cgccggcccg gacgacacgg
     5161 gcccgatcgt gatcaacggc gcgccgatgt cctacggggg cggagcctgg caggaaggcg
     5221 agagcgacaa cccgatgcgc tacagcggcg gcatgctcgg cggctcgtgg ctcgcctcgt
     5281 tcgccgccga catgggcaac ggggtcttcg acggcgccta cctggtcgag aatttcgaaa
     5341 gcc
//
LOCUS       DBNGSB010000170         3626 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_4260330, whole genome shotgun sequence.
ACCESSION   DBNGSB010000170 DBNGSB010000000
VERSION     DBNGSB010000170.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_4260330"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..121
                     /locus_tag="AC159B_04940"
     CDS             <1..121
                     /locus_tag="AC159B_04940"
                     /inference="COORDINATES: protein motif:HMM:NF022519.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YlqD family protein"
                     /protein_id="HIR3377125.1"
                     /translation="AQGTVESLVEVNVGDNLFTKLGRTEIVVKDGIIMEIREE"
     gene            220..765
                     /gene="rimM"
                     /locus_tag="AC159B_04945"
     CDS             220..765
                     /gene="rimM"
                     /locus_tag="AC159B_04945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HIR3377126.1"
                     /translation="MAAGYDVEGTKGPSFIVVGRVGRPHGLRGEMRLQPDTDFPERLF
                     ALTQVTLLKNGESMEAGVVSIRPHGTAMLLKVEGIDTIAGASRWRDAAVAVRRETAAP
                     LDAGRHFVSDVLGLRVETEDGNFLGTVAEVLRTGSNDVYVVRAEKREVLVPAISTVVL
                     AIDVAAARIVIRLMPGMLETG"
     gene            767..1501
                     /gene="trmD"
                     /locus_tag="AC159B_04950"
     CDS             767..1501
                     /gene="trmD"
                     /locus_tag="AC159B_04950"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HIR3377127.1"
                     /translation="MRIDIVTIFPEAFTPIEVSLLGRAREAGYLQIKVWNLREFTTDR
                     HRQVDDAPYGGGAGMVMKPEPFFEAVDAIRAASPEATPRIILTSPQGIPLTHALAQEL
                     ANEQHLVLLCGHYEGIDERVRESLATDEISVGDYVLTGGELPAMVIVDAVARFVPGVV
                     GDAASVEADSFSEGLLDYPHFTRPAEYRGMQVPQVLLSGDHESIRRWRQAQRLRRTLE
                     RRPDLLKAEALGEEDRRLLDELDVED"
     gene            1570..1911
                     /gene="rplS"
                     /locus_tag="AC159B_04955"
     CDS             1570..1911
                     /gene="rplS"
                     /locus_tag="AC159B_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HIR3377128.1"
                     /translation="MEKVALIEREQMKKKLPDFTPGDAVRVHVKVVEGGRERVQAFEG
                     VVIGRKSGGIRETFTVRRISHGVGVERTFPLHSPRIDRIDIVRKGKVRQAKLYYLREK
                     VGREARIKESR"
     gene            1933..2577
                     /gene="lepB"
                     /locus_tag="AC159B_04960"
     CDS             1933..2577
                     /gene="lepB"
                     /locus_tag="AC159B_04960"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR3377129.1"
                     /translation="MRLEFLDRLPLSIPSLILVLAGVLIIARIVIRRVQSIPSHWRSS
                     VVETLDASIIAALLSLLIITFVVQAFYIPSGSMEPTLMVGDRILVSKLSYRFGTVERG
                     DVIVFHYPLNPGKDFVKRVVGLGGETVELRDGVVLINNTPIKELYPTALGGGDRSCTS
                     SYGPQRVPAGQIFVLGDNRCNSEDSRFFGFVPRVNVVGRAGFIYWPPPRLSLVQ"
     gene            2588..3403
                     /locus_tag="AC159B_04965"
     CDS             2588..3403
                     /locus_tag="AC159B_04965"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIR3377130.1"
                     /translation="MTIRGPLRPSDMRRLSLAQLSTLVRLMEVVDPAILRVLSRDRRQ
                     GARALVARLQVRMSRMYRELSRIESLYAVERDRRGAGFDVIAGVDEAGVAPLAGPVVA
                     AAVILPEVVDLPGLDDSKRLDAGERAALYERIRRTALAIGTGVAVVEEIDRINILQAT
                     RLAHRRAIAGLGIRPHLVMLDGRIPADVSVPQLVIIDGDATCASIAAASIVAKVTRDR
                     MMAGFADRYPGYGFAGHKGYPTKAHREAIARLGLTPIHRRSFVHVEQQLIPIG"
     gene            complement(3393..>3626)
                     /locus_tag="AC159B_04970"
     CDS             complement(3393..>3626)
                     /locus_tag="AC159B_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3377131.1"
                     /translation="GNMIEQQVMYAPMDIQRAYQVTFFLQDAVGSLVGAQKGMIAYRA
                     AVSNRTETHLPFGSLTMRFTPLGAGQIRVLVSR"
BASE COUNT          659 a         1075 c         1198 g          694 t
ORIGIN      
        1 tgcacagggc accgtcgaga gtctggtgga agtgaacgtg ggcgataact tgttcaccaa
       61 actgggccgc acggagatcg tggtcaaaga tgggatcatc atggaaatta gagaggagtg
      121 agagccatga ggcggaggga accctgggga cctggacccg aagagcggct aaggagtgac
      181 ttagttacgg gtccctagtc cccagggtcc cctcgcctca tggctgctgg atacgacgtg
      241 gaaggaacga aaggaccctc attcatcgtg gtcgggcgcg taggtcggcc gcacggcctg
      301 cgcggggaga tgcggctcca gccggacacg gattttcccg agcggttgtt cgcgctcacg
      361 caggtcacgc ttctcaagaa cggcgagtcg atggaagcgg gcgtcgtttc gatccgaccg
      421 cacggaacgg cgatgctgct caaggtcgag ggcatcgata caatcgccgg cgcgtcgcgt
      481 tggcgggatg ccgcggtagc ggtgcggcgg gagactgcgg cgccgctcga cgccgggcgg
      541 cactttgtct ccgacgtgct gggactgcgc gtggagaccg aggacgggaa tttcttaggg
      601 accgtggctg aagtgcttcg caccggcagc aacgacgtct acgtggtcag ggccgagaag
      661 cgcgaagttc ttgtgccggc gatcagcacc gtcgtgctcg ccatcgacgt cgccgccgcg
      721 cgtatcgtca ttagactgat gcccgggatg ctcgagacag ggtagcatgc gcatcgacat
      781 tgtgacgatc tttccggagg cgttcacgcc gatcgaggtc agcctgctgg gacgggcccg
      841 agaagccggt tacctgcaga tcaaagtctg gaatcttcga gagttcacga cagaccgcca
      901 ccgccaggtc gatgacgcac cgtacggtgg aggcgccgga atggtcatga agcctgagcc
      961 cttcttcgag gccgttgatg ccatccgcgc cgcctcgccg gaggctacgc cgaggatcat
     1021 tctgacctcg cctcagggca ttcccttgac gcacgcgctt gcccaggaac ttgcgaatga
     1081 gcagcacctg gtcttactgt gcgggcacta tgaggggatc gatgaacgag tccgcgagag
     1141 tctcgcgact gacgagatct cggtcggcga ttacgtgctg acgggagggg agcttccggc
     1201 gatggtgatc gttgacgccg ttgcgcggtt cgtcccgggt gtggtcggag acgcggcatc
     1261 cgtcgaggcc gactcgttca gcgaggggtt gctggactac ccgcacttta cacgcccggc
     1321 cgaataccga gggatgcagg tgccgcaggt tctcctatcc ggagatcatg agtcgattcg
     1381 ccgctggcgc caggcgcagc gtcttcgccg cacgcttgaa cgccgcccgg atctgctgaa
     1441 ggcggaggcg ttgggagaag aggaccgcag actgctcgat gaactcgatg ttgaggatta
     1501 gacagaaggg agcgcgggct gtgctataat tcaacgccgt gaccgcgtaa actgatccgg
     1561 gggatggcaa tggagaaagt ggcactcatc gaacgggaac agatgaaaaa gaagctcccc
     1621 gacttcacgc ccggcgacgc cgtgcgtgtg cacgtcaaag tcgtcgaggg cgggcgtgag
     1681 cgcgtgcagg cgttcgaagg agtggtcatc gggcgcaaga gcggggggat tcgcgagacg
     1741 ttcaccgtgc ggcggatcag ccacggggta ggtgtggagc gcacttttcc gctgcattcc
     1801 ccgcgcatcg atcgcatcga catcgtacgc aagggcaaag tccggcaagc gaagctctac
     1861 tatctgcgcg agaaagtcgg gcgcgaagcc cgcatcaagg agagtcgcta gaggcccagt
     1921 tccccggcca cgatgcgtct ggagtttctc gaccgcctgc cactcagcat ccccagcctg
     1981 attttggttc ttgcgggcgt gctcatcatc gcacgcatcg tgatccggcg cgtgcagtcc
     2041 attccttccc actggcgcag ctcggtcgtg gagacgctgg acgccagtat catcgcggca
     2101 ttgctgagcc tgctcatcat cacgttcgtc gtccaggcat tttacattcc ctccggctca
     2161 atggagccga cgctgatggt tggcgatcgc atcctcgtca gcaaactgtc ctacaggttt
     2221 ggaacggtcg aacgcggcga cgtgatcgtc tttcattacc ccctcaatcc aggcaaggat
     2281 ttcgtcaagc gggtggtggg actcggcggc gaaacggtgg aacttcgtga tggcgtggtg
     2341 ctcatcaaca acacacccat caaagaactc tatccgaccg ccctcggcgg cggtgaccgt
     2401 tcctgtacga gcagttacgg tccccagaga gtgcccgcgg gccagatctt cgttctcggg
     2461 gacaaccgct gcaacagcga ggacagccgg ttcttcggtt ttgttccccg tgtgaacgtc
     2521 gtgggcaggg cggggttcat ctactggcca ccgcctcgat tgagcctcgt gcaatgagac
     2581 tcgtggcgtg acgattcgag gtccgctccg tccgtcggac atgcgtcggc tgtcgctggc
     2641 gcaactctcc acgctggtcc ggctgatgga ggtggtcgac cccgcaatcc tgcgcgtcct
     2701 gtcccgtgac cggcgtcagg gtgcgcgcgc gctcgtggcc cgattgcagg tgcgaatgtc
     2761 gcgaatgtat cgggagctct ctcgcatcga atcgctctat gccgttgaac gcgatcgccg
     2821 cggagccggg tttgatgtga ttgccggtgt cgatgaggcc ggcgtagcgc cactggccgg
     2881 gccggtggtt gcggctgctg tcatcctgcc tgaggtcgtc gaccttccag gattagacga
     2941 ttccaagagg ctcgatgccg gtgagcgcgc ggcgctctac gaacggatcc gccgtacggc
     3001 cctcgcgatc ggcacgggcg tggcagtcgt ggaagagatc gaccgtatta acatccttca
     3061 agcgacgcgg ctggcccacc gacgggccat cgcgggactt ggtattcgtc cgcacctcgt
     3121 gatgctggat ggccgcatcc cggccgatgt ctccgtcccg cagctcgtga tcatcgatgg
     3181 tgacgccacc tgtgcctcga ttgcggcggc gtcgatcgta gcgaaggtca cgcgagatcg
     3241 catgatggct gggttcgccg accgataccc cggatatggg tttgcagggc acaaagggta
     3301 tcctacgaag gcgcaccgcg aagccattgc caggctagga ctgaccccga ttcaccgccg
     3361 ctcgttcgtg cacgtcgaac agcagctgat tcctatcggc tgacaagcac ccggatctgc
     3421 cccgcgccca gcggggtaaa gcgcatcgtc agcgatccaa acggcaggtg cgtttctgtt
     3481 ctgttgctca ccgcggcgcg gtaggcgatc attccctttt gcgcgccgac gagcgaaccc
     3541 accgcatctt gcaggaagaa ggtcacctga tacgcccgct ggatgtccat tggcgcgtac
     3601 atgacctgct gctcgatcat gttgcc
//
LOCUS       DBNGSB010000171         3498 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2575456, whole genome shotgun sequence.
ACCESSION   DBNGSB010000171 DBNGSB010000000
VERSION     DBNGSB010000171.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2575456"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="AC159B_04975"
     CDS             complement(<1..862)
                     /locus_tag="AC159B_04975"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HIR3377132.1"
                     /translation="MSAPLLGQFDPEFTSIMEDVMALGRRVFRTSDARTFAVSATGRG
                     GMEAAIASLVEPEDRVLVANCGRFGDLFVDLATRYGARVSQVTAEWGHVIDPQQIEAS
                     LKRTLAKVVAIVHGETSTGMLQPQMEDIGRLCQERDALLVVDAVVTLGGVPVEVDNWQ
                     IDMCTSGTQKCLGCPSGMAPITYNARAEKALAGRKNPVVSNYFDLSQLQRYWSPERLN
                     HHTLPTSMTYGLREALRLVADEGLEERWSRHRRTGNALKAGLEAMGVQLFGDRQHRLP
                     MITAMHVPEGV"
     gene            complement(943..2235)
                     /locus_tag="AC159B_04980"
     CDS             complement(943..2235)
                     /locus_tag="AC159B_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbE family protein"
                     /protein_id="HIR3377133.1"
                     /translation="MKNQPGIAEHIQKANDEFIARISKAVPSLVRVRQAREVIPALTG
                     QTVLHAGPPITAERLCGPMRGAILGAAVLEGWAKTPEDAVKLLDGERIGLHCAHDSNA
                     VGPMAGIISPSMPVFEVRDETSNTTAYCTINEGIGTVLRFGAYDATVIDRLRWIAQAL
                     APVLDRALQNLQGLPLLPLMNRALAMGDEMHQRNVAATGLLFRALAPALAKQSASDAL
                     ARILEFLGQNDQFFLNIAMAACKATMNATRGISYCTAVTAMSRNGVDFGIRVSGLGDT
                     WFVSPSPVPDGLYFPGYGAGDANRDMGDSAIVETAGLGAFAMAAAPAVAGFVGLASAR
                     EALAATRAMSEITLGSSPHFKIPALDFEGTPTGIDIRRVIEVDTTPIINTGIAHRLPG
                     VGQIGAGVVHAPIETFQQALAAFVDRYASPAATQDRRG"
     gene            complement(2232..>3498)
                     /locus_tag="AC159B_04985"
     CDS             complement(2232..>3498)
                     /locus_tag="AC159B_04985"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HIR3377134.1"
                     /translation="MSTPRISLRRLQQRLEELGAIGQDPDGGITRPPFSPAHVDAVRR
                     CAEWMREAGLQTGVDEFGNLLGLKGERGPALLAGSHLDTVPQGGMFDGALGVVAAIEC
                     AQALHEGGRTLRHQVVAIAFAEEEGYAFGSGTLSSRSLLGELPRDRFAELVDGGGRTY
                     EEHLRARAHGLPSSQVPAQVRAYLELHAEQGPHLERMGKQVAAVDQIVGSVRTVVTFE
                     GRAAHAGTTPMGMRADALLGGAEFVLAVNEIATASDEHGVGTVGELHAFPGARNVIPG
                     KATLTVEFRSHDDSWLTSARGRAEEAAKKIEDGRRLSVLVSRWSTSRVQLLDSEVRQT
                     ILDAMRSTGHDPVTLSSWAGHDAGILARYVPSGMIFVTSAGGISHAPHEHTALPAVAA
                     GTEVLLESLISLDAIKGEALTLPLAYAKT"
BASE COUNT          639 a         1145 c         1056 g          658 t
ORIGIN      
        1 ggacaccctc aggaacgtgc attgcagtaa tcatcggcag ccggtgctgt cgatcgccga
       61 acagttggac tcccattgct tccaatccag ctttaagcgc attaccagtg cggcggtggc
      121 gtgaccatct ttcctcgagt ccttcgtcag cgaccaaccg cagcgcttcg cgcaacccgt
      181 aggtcatgct ggtgggcaac gtgtggtggt tcaatcgttc cgggctccaa taccgctgaa
      241 gctgcgacag gtcaaagtag ttgctcacca ccgggttctt gcgaccagcg agcgcctttt
      301 cggctcgagc attgtaggtg atcggcgcca tgccggatgg acaccccagg catttctgcg
      361 tccccgacgt gcacatatcg atctgccagt tgtcgacttc caccggcacc cctccgagtg
      421 tcacaacggc gtcgacaacg agtagtgcat ctcgctcttg gcagaggcgg ccgatgtctt
      481 ccatctgggg ctgcagcatc ccggtcgacg tctcgccgtg cacaatcgca acgacctttg
      541 cgagggtacg cttcagtgat gcttcaatct gctgtggatc gatgacgtgt ccccactccg
      601 cagtgacctg cgagacacgg gcgccatagc gagtggcgag atccacgaag aggtcgccga
      661 accgtccgca gttcgccacg agcacgcgat cctcaggctc cacaagtgac gcgatggccg
      721 cctccattcc accacggcct gtcgccgaga cggcgaacgt gcgcgcgtcg gacgtccgaa
      781 agacacgccg gccgagcgcc atgacgtctt ccatgatcga agtaaactcg ggatcgaact
      841 gaccgaggag tggcgccgac atggcgcgca gaacgcgggg gtcggggttc gtaggtcccg
      901 tgccacacaa caagcgcgcc ggtggttcca cgcgtccctt tcctaccctc ggcggtcctg
      961 ggttgccgcc ggactcgcgt atcggtcaac gaacgcggcg agagcctgct gaaaagtctc
     1021 gattggcgcg tggacgaccc cagcgccgat ctgcccaact ccggggagtc tgtgggcgat
     1081 gccggtatta atgatcggag tcgtatccac ttcgatcact ctgcgaatgt cgattcccgt
     1141 cggggtgcct tcgaaatcca gcgcaggaat cttgaagtgt gggctcgatc ccagcgtaat
     1201 ctcactcatg gcgcgagtgg cggcgagggc ttcgcgagcc gatgccagtc cgacgaaccc
     1261 tgcgacggcc ggggccgccg ccatcgcgaa ggctccgagc ccggcggttt cgacgatcgc
     1321 gctatcgccc atatcccggt tggcatcgcc ggccccgtag ccaggaaagt acagaccatc
     1381 tggaacgggc gagggcgaga caaaccatgt atctcccagc ccgctcaccc gaatcccaaa
     1441 gtccaccccg ttgcgactca tcgccgtgac cgcggtgcag tacgagattc cgcgtgtcgc
     1501 gttcattgtc gctttgcatg ccgccatggc gatgttcagg aagaactggt cattctgacc
     1561 caagaactcc aggatcctgg cgagagcgtc cgaggcgctc tgcttcgcca gcgccggcgc
     1621 cagcgcccga aacagcaagc cggtggcggc cacgttccgc tgatgcatct cgtcgcccat
     1681 ggccagcgcg cggttcatga gtggaagaag cggaagtccc tgaaggttct ggagggctcg
     1741 atcaaggacg ggcgccaggg cttgggcaat ccagcgcagc cggtcgatca ccgttgcatc
     1801 ataggcgcca aaccgcagca ctgtcccgat gccttcattg atagtgcaat aggcggtagt
     1861 gttcgacgtc tcgtcgcgga cctcgaacac gggcatcgag ggtgagataa tgcctgccat
     1921 cggccccacc gcattcgagt catgcgcgca atgcagaccg atgcgctctc catcgaggag
     1981 ctttaccgca tcctcgggcg tcttcgccca cccttccagg acggccgccc cgagaatcgc
     2041 gccgcgcatc ggcccacaga gtcgttcggc ggtgatgggg ggacctgcgt gcaggaccgt
     2101 ttgaccggtg agagcgggaa tgacttcgcg cgcttgcctg acgcggacca gcgaaggcac
     2161 tgctttgctg atgcgagcaa tgaattcatc gtttgccttc tggatgtgct cggcgatgcc
     2221 tggctggttc ttcatgtctt cgcgtacgcc agcggcagcg tgagcgcttc tcccttgatc
     2281 gcgtccaggc tgatcaggga ttcgagcagc acctccgtcc cggctgccac agccggcaag
     2341 gccgtgtgct catgcggcgc gtgactgatc ccgccggcgc tggtaacgaa aatcatcccg
     2401 gacggtacgt atcgtgcgag aattcctgcg tcgtgtccag cccagctcga gagtgtcacg
     2461 ggatcgtgcc ctgttgagcg cattgcatcc aggatcgttt gccgaacctc cgaatccaac
     2521 agctggaccc gagaagtgct ccatcgcgat acgagaacac tcagcctcct cccgtcttcg
     2581 atcttcttgg cagcttcctc tgcgcgcccg cgggctgatg taagccagga gtcgtcgtgg
     2641 ctacgaaact ctacagtaag cgtcgctttc cccgggatca catttctcgc accaggaaac
     2701 gcgtgcagtt caccgaccgt gccgactcca tgctcgtctg aagccgttgc gatctcgttt
     2761 accgcgagga cgaattctgc gcccccgagg agcgcatccg ctcgcatccc catgggcgtc
     2821 gttcccgcgt gggcggcacg accttcgaac gtgaccactg tacgcacgct gccgacgatt
     2881 tggtcgactg ccgcgacctg cttacccatg cgctcgaggt gcggaccctg ttcggcgtgg
     2941 agttcgagat aggctcgcac ctgcgcaggg acctgcgagg atggaagccc gtgcgcgcgc
     3001 gcccgaagat gctcttcata tgtgcgcccg ccgccatcca ccaactcggc gaaccggtcg
     3061 cggggcagtt cgccgaggag cgagcgggat gataatgtgc ccgagccgaa cgcataccct
     3121 tcttcttcgg cgaaggcgat cgcgacgact tgatggcgca gcgtccggcc gccttcatgc
     3181 aaagcttgtg cacactcaat cgcagccacc acgccgaggg cgccatcaaa catgccaccc
     3241 tgcggcaccg tgtccaggtg agatcctgcg agcagcgccg gtccacgctc acccttgagc
     3301 ccgaggaggt tgccaaactc atcgaccccc gtttgtaacc cagcctcgcg catccattcc
     3361 gcgcaccggc gaacggcgtc gacgtgcgcg ggcgaaaatg gcgggcgtgt gatgccaccg
     3421 tccggatctt ggccgatcgc accaagttcc tcgagccgct gctgcaaacg gcgcagtgag
     3481 attctgggcg tagacata
//
LOCUS       DBNGSB010000172         2556 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_276204, whole genome shotgun sequence.
ACCESSION   DBNGSB010000172 DBNGSB010000000
VERSION     DBNGSB010000172.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_276204"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..713
                     /locus_tag="AC159B_04990"
     CDS             162..713
                     /locus_tag="AC159B_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3377135.1"
                     /translation="MRGRRLHLTVLSSTLAILALGLSFAYAQQAMNSSLFSGQPAIQI
                     TNPPLYYVWFDQSGWHVRWSSIGPGVFSGQIVTNGQVMDVRSQGRLASWVVPAGGRLV
                     FLTGTVGGIDGFDFRTTGDALTFNLLQNQRLISPAQVVIGSGNARAFAMPFSVTTSAF
                     AAGTAVPSWIDPSDHVGAPGEGR"
     gene            complement(733..1746)
                     /locus_tag="AC159B_04995"
     CDS             complement(733..1746)
                     /locus_tag="AC159B_04995"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006446644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3377136.1"
                     /translation="MADDLFDITIVGAGPTGLFAAYYAGFRGLRTKLIDSQPDLGGQV
                     TALYPDKFIYDVAGFPKILGKDLVTNLVEQAMQYKPAIHLNENVQTFSRTEDDLKLGT
                     DLGTHRTKVVLITAGIGAFTPKTFKRPELDQWMGRGLHFAVRSKEDFRGKRLLVIGGG
                     DSALDWALGLVDYAKETALIHRRDQFRAHEDSVKKLLSSPIKVKVFYELRTIRGDGQV
                     REAVIFNNKTQAEEVLPVDAVLAFLGLVSNLGPIREWGMEIDDDSIVVNTKMETTIAG
                     LYAAGDITAYPGKLKLIATGFGEAATAVNNAMTYINPKASAFPGHSSSIMEQKEKAGA
                     KTS"
     gene            1881..>2556
                     /locus_tag="AC159B_05000"
     CDS             1881..>2556
                     /locus_tag="AC159B_05000"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HIR3377137.1"
                     /translation="MKVTARPGSLLTPEQMERYARQIILEDVGIAGQRKLLDSKVLLI
                     GAGGLGSPAAIYLAAAGVGTLGIVDGDRVDLTNLHRQVMHFTHDIGRPKTQSANRTLE
                     DINPDVRVIPFQTVLTSENAFEIIKEFDVVVNGSDNFPTRYLVNDACVMLGKPMVDAS
                     ILKWEGQATVFLPGHGCYRCLFPTPPPPGAVPSCAEGGIVGAVAGHMGTLQATEAIKI
                     LLGKGAT"
BASE COUNT          475 a          744 c          809 g          528 t
ORIGIN      
        1 gcgtagcagg ccggcggcgc acaaggccgg agcggggcga aggccgttga atctcgctcg
       61 ttgcgacttg ataagagggg gtaatagtgt agagaaccca ggggcggcag tttgcgtaat
      121 cctggtgaaa cgttgacgta catatgatgg agggaattct catgcgcggt agacgtttgc
      181 atctcacggt gctgagcagc actctggcga ttctggcgct cggcctgtcg ttcgcctacg
      241 cgcagcaggc catgaactcg tcgctgttca gcggccagcc agccatccag atcacaaacc
      301 cacctcttta ctatgtctgg tttgaccaga gcggttggca cgtgcgctgg agcagcattg
      361 ggccgggtgt gttcagcggc cagatcgtca caaacggtca ggttatggac gtccgttctc
      421 aggggcgttt ggcttcctgg gtggtgccgg caggggggcg actggtcttt ctgaccggaa
      481 cggtcggagg catcgacggg tttgacttta gaacgaccgg cgacgcccta acgttcaact
      541 tgctgcagaa ccagcggctg atctcacctg ctcaagtcgt gatcggcagc ggcaacgcgc
      601 gtgccttcgc gatgccgttc agcgtgacga catcggcgtt tgctgcaggg acggcagtgc
      661 cgagttggat cgaccccagt gaccacgtgg gtgcaccggg cgaaggacgg tagaaagacg
      721 ctgaggcggg tgctacgagg tcttggcgcc tgccttttct ttttgttcca tgatcgaaga
      781 tgagtggccg gggaaggccg aggccttggg attgatatag gtcatcgcgt tgttgaccgc
      841 ggtggccgct tctccgaacc cggtggcgat gagcttcagc ttcccaggat acgccgtaat
      901 gtctccggcg gcgtagagtc ccgcaatcgt cgtttccatc ttggtattga cgacgatgga
      961 gtcgtcatcg atttccatgc cccactcgcg gatcggtccc aggttggata cgaggccaag
     1021 aaatgccagg acggcgtcaa ccggcaaaac ttcctcggcc tgcgttttgt tgttgaagat
     1081 caccgcttcc cgcacctggc cgtcgccccg gatcgttcgc agttcgtaga acaccttcac
     1141 cttaatgggt gagctcaaaa gtttcttgac gctgtcctca tgcgctcgga actggtcgcg
     1201 gcggtgaatg agggccgttt cctttgcgta gtccaccaat ccgagcgccc agtccagcgc
     1261 ggagtcgcca ccgccgatga cgagcaggcg cttgccgcgg aagtcttcct tggaccgcac
     1321 ggcaaagtgc aacccccggc ccatccactg gtcgagttcc ggtcgcttga acgtcttggg
     1381 cgtgaaagcg ccgataccgg ccgtgatgag cacgactttc gtgcggtgcg tacccagatc
     1441 ggtgcccagc ttcaggtcgt cctcggtccg agagaacgtc tgcacatttt cgttcaggtg
     1501 gatggccggc ttgtactgca tggcctgctc gacgaggttc gtgaccagat ccttgccgag
     1561 gatcttgggg aatcccgcga cgtcgtagat gaacttgtca ggatagagcg ccgtgacctg
     1621 ccctccaaga tcgggctggc tgtcgatgag cttggtgcgc aggccgcgga agcccgcata
     1681 gtatgccgcg aagagtcctg tgggccctgc gccgacgatc gtgatgtcga agagatcgtc
     1741 tgccatgttc gataccgtcc ctcccgaaaa gccgtgctat tatatcattt cacccactcg
     1801 actcgcggac tataccgcta tgatagaatt taccaggctg attttccgtt catccaagta
     1861 gtgtgagtga ggaggcggca atgaaggtca cggcacgccc tgggtccttg ctgaccccgg
     1921 agcagatgga gcgctacgcg cgtcagatca tcctcgagga cgtggggatt gctggtcagc
     1981 gcaagctgct cgacagcaag gtgctgctca tcggcgccgg cgggttaggg agcccggccg
     2041 ctatctacct cgccgccgcc ggcgttggga cactcgggat cgtagacgga gaccgcgtgg
     2101 atctcaccaa cctgcaccgg caggtcatgc actttaccca cgacatcggc cgtccgaaga
     2161 cgcaatcggc caaccggacc ctcgaggaca tcaaccccga cgtgagggtc atcccattcc
     2221 agaccgtgct tacgagcgag aacgcgttcg agatcatcaa ggagttcgat gtcgtggtta
     2281 acgggagcga caacttcccg acgcggtacc tggtgaatga cgcctgtgtg atgcttggca
     2341 aaccgatggt cgacgccagc attcttaaat gggaaggcca agccaccgtg ttcctaccgg
     2401 ggcacggttg ctaccggtgt ctcttcccca cccccccgcc tcctggtgcg gtgccgagct
     2461 gcgcggaggg cggaatcgtc ggcgcggtgg ccggccatat gggcacgctg caggccacgg
     2521 aagccatcaa gattctgctg gggaaaggcg ccacgc
//
LOCUS       DBNGSB010000173         4777 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_276552, whole genome shotgun sequence.
ACCESSION   DBNGSB010000173 DBNGSB010000000
VERSION     DBNGSB010000173.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4777)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_276552"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="AC159B_05005"
     CDS             complement(<1..566)
                     /locus_tag="AC159B_05005"
                     /inference="COORDINATES: protein motif:HMM:NF025687.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HIR3377138.1"
                     /translation="MAPRQQSAGTSGIIAGISLAVVFFLFITSRAGPETFADPANALP
                     FITQNSGRLRTIALLALITISFAVIFVAGLAARLRDKTPTRATGVLYFGLLGLIGHGI
                     GALMFWVGVPALVAYAAKDQVAVSHAWVAFGALNGSLDGFGNLFIGLSTLLAGWAIVG
                     AGAMSSTLGWFGVVAGVVTALAAVAPGN"
     gene            1282..1830
                     /locus_tag="AC159B_05010"
     CDS             1282..1830
                     /locus_tag="AC159B_05010"
                     /EC_number="1.16.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HIR3377139.1"
                     /translation="MQTKTETKTRRRPELRGLTAPRQLATATDLKAEDVRAVTETVNP
                     LIADAFALYVKTKNFHWHLSGPRFRDYHLLFDEQAAAILESIDILAERVRKLGGLTIR
                     SIGHIARLQTVEDDNSEFVPASEMINRLLADNKKMAEQQREAIEVCDEHDDTPTGNIL
                     QEILDQTERRIWFLFELSQADE"
     gene            complement(1911..2744)
                     /locus_tag="AC159B_05015"
     CDS             complement(1911..2744)
                     /locus_tag="AC159B_05015"
                     /inference="COORDINATES: protein motif:HMM:NF014899.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DODA-type extradiol aromatic ring-opening family
                     dioxygenase"
                     /protein_id="HIR3377140.1"
                     /translation="MPIAFACVAPHGEPIIPALSSAAERRRYAVTTQAMQRLAREARA
                     ARPHLLVIATPHNLRLRDHIGVITAENASGSLPMFGKGPARLPIRVKCDVQFADVLLK
                     QATRRRLPAIGANYATASGRYSNMPLDWGTLVPLWFFMRGHRVPPKLVVVTPSREISL
                     AENLRFGSLIADVAQGSNRRVIFVASADHAHAHRKSGPYGFSPAAARYDALMVDAIKG
                     NDLRSLLRIDMNLVRRAKPDSLWQLVMLAGIAERLKLRPDLYSYEVPAYYGMICAAFR
                     R"
     gene            2846..4357
                     /locus_tag="AC159B_05020"
     CDS             2846..4357
                     /locus_tag="AC159B_05020"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR3377141.1"
                     /translation="MPPTLLEAREISKSFPGTRALDRVHFEVLPGEVHALVGENGAGK
                     TTLMMILAGVLQPDEGAILWDGVAVPLTDPRAAQGLGIGTVFQELSLVPGLSIGENIF
                     LTRAPARTALGLVEWSRVYADAQVALAQLGINVDPMLPVNALAMGARQMVEIAKALSL
                     RCRMLLLDEPTSALSPGEVDRLFTAIRALRDQGLGIVFITHRIAEVFQIADRVTVLRD
                     GHTVATLEMSGTTPDEVVHYMVGRNLGKSMRPPPPSTSTPILEVSGLSRPPILQGISF
                     SLYAGEILGVAGLPDSGREELGRTLFGIEPPLSGAIRVEGRALSIRSPQDALRAGIGY
                     LPPDRRTLGLFLRMPVKDNIVVTALDQITHSGIVDDGRAAAIAARYVDQLQVHSPSVS
                     QVVGRLSGGNQQKVALSKWLFRRPRVLIADGPTIGVDVGARMAIHQLLYQLAAEGMSI
                     VLLSTDLPELLGLSNRIMVMNGGRVAGVLAGNEASEQRVITLAAGQRLGPKNV"
     gene            4357..>4777
                     /locus_tag="AC159B_05025"
     CDS             4357..>4777
                     /locus_tag="AC159B_05025"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3377142.1"
                     /translation="MDQKPSVASASRAGTAVRLRSEKASFAHLLQMREVLLFAVILAL
                     SLAISRLTPYFLTSGNLKAMLIGMVPTAIIAVGTAIVLVGGGLDLSVGSVMALSGTVA
                     GLLMLTGTPIWLALSLTLMVGGLIGLANGLVITRIRVN"
BASE COUNT          959 a         1434 c         1463 g          921 t
ORIGIN      
        1 tggttccccg gcgccaccgc ggcgagcgct gtgaccacgc ctgcgactac cccgaaccat
       61 ccaagtgtcg aactcatcgc ccctgcgcca acgatcgccc agccggccag caaggtggac
      121 agtccgatga aaagattgcc gaacccgtca agcgagccgt tcagcgcgcc gaaggccacc
      181 cacgcgtggc taaccgccac ctgatctttc gccgcgtaag cgacgagcgc gggcacaccc
      241 acccagaaca tcagggcgcc gatgccgtgc ccgatgagcc ctaggagtcc aaagtagagt
      301 acccccgtgg cccgggtcgg agtcttatct cgaagcctgg ccgcgagtcc tgcgacaaag
      361 atgacggcga atgaaatcgt aataagcgcg aggagtgcaa tggtgcgaag gcggcccgag
      421 ttctgcgtta tgaacggtag agcgtttgcg ggatctgcga acgtctcggg tccggctcgg
      481 ctggtgatga agaggaaaaa gacgacggcg agtgagatac ctgcaatgat ccctgaggtt
      541 cctgcggatt gctgacgcgg ggccatgtca tacctccaag cccgccccgg gtttgccgta
      601 cgaagaggcg gtgcccttca agcggcttcg gggcaaccat gtggattccc ccgtcggaac
      661 gtgaagttag cgaaaaatct gcgggtgcgc cgaggcgagt gaggggtagc tcgcgcgcca
      721 ccggccgtct gtccctcgtt ggaccgctat tgttacacgg ttgcgctccg tgagcgcgga
      781 tccccccgag cgaaggatcg tggtagcgac aaacgatacc gtgcctctgt ccccgctgag
      841 acgcactcca ggactgttga gttggaaacc acgaagacgc gatcgttcga gcggctgggg
      901 ccaatcacga gggacattag caagaaatcg ttctgcccag gcctgcgcct gaggttgcga
      961 ggcatgcgcg aaggactgat aaacctggat gatggcgttc actgctagat cgcgggcgtt
     1021 cggcgggaac cccacaacaa ttccgccggc aacaagacta tctattgctg ctcgtgacca
     1081 atgccatggc ggaacgtcct caaaaccctg ggcgacgcca gacaccgcaa gtaagcccac
     1141 cagaacgacg gatacgatca gaactctcat ggcgcgtctc cgatacaggc atcctctatc
     1201 cttcataata caccggtcct gttgcgatac tcccgaaggt ggggtaagaa ctctcagtat
     1261 cccaccgtga ggaggccaac catgcaaacg aagacggaaa cgaaaaccag acggcgaccg
     1321 gagttgagag ggctcaccgc cccgcgccag ctcgctaccg ccaccgatct gaaggctgag
     1381 gatgttcggg cagtcaccga gacagtgaac ccgctgatcg cggatgcgtt cgctttgtac
     1441 gtgaagacga agaacttcca ctggcatctc tcgggcccgc gatttcgcga ctaccatctc
     1501 ctgttcgacg agcaggccgc agccatccta gaatcgattg atattctggc ggagcgcgtg
     1561 cgaaagcttg gaggcttgac gatacgcagt atcggccaca ttgcccgtct ccagacggtc
     1621 gaggatgaca acagcgagtt cgtacctgcg tccgaaatga tcaacagact attggcagat
     1681 aacaagaaga tggccgagca gcagcgcgag gcgatcgaag tctgcgacga gcacgatgac
     1741 acaccgacgg ggaacatcct ccaggagatc ctggatcaaa ctgagagacg gatctggttc
     1801 ctgtttgaac tcagccaggc cgacgagtag ctgcttccta actacacact caaggatggg
     1861 ggccggctcc agccggcctt tttcttgttg gtcagtactt ccttcaccac ttatcttcga
     1921 aacgctgcgc agatcatgcc gtagtaggcc ggcacttcgt aggagtacag gtcgggacgc
     1981 agcttcagac gctcggcgat ccctgcgagc atcaccagct gccagaggct gtccggtttg
     2041 gcccttctaa ccaggttcat atctattcgc aggagggaac gcagatcgtt ccccttgatc
     2101 gcgtcgacca tcagggcatc gtaccttgcc gcggcagggc tgaagccata cggaccagac
     2161 tttctatgcg catgtgcgtg gtcggcgctc gccacaaata taacccgcct gttcgatcct
     2221 tgtgccacgt cggcgatcag cgagccgaac cggagattct cagccaacga aatttctcgt
     2281 gagggtgtga cgacgaccag cttcgggggc actcgatgac cgcgcatgaa aaaccacagg
     2341 gggacgagcg tcccccagtc cagcggcatg tttgagtagc gaccggaagc cgtcgcatag
     2401 tttgcgccga tcgcggggag ccgccggcgt gtggcctgtt tgagcaatac gtccgcaaat
     2461 tgcacgtcac atttcacacg aataggtagt cgtgccggtc ccttcccgaa catgggcagt
     2521 gagcccgaag cattctcggc ggtgatgact ccgatatgat cccgtagccg caggttgtgg
     2581 ggcgtcgcaa tgacgaggag atgtggccgc gcggccctcg cttcgcgcgc cagccgctgc
     2641 atcgcctgtg tcgtgactgc atagcgccgt cgctcagcag ctgacgacag agccggaatg
     2701 atcggttcac catgcggggc aacacaggcg aatgcaattg gcatggcatc gccctttctc
     2761 ggcggtcaaa gggcatcctg ccgcagggag gggacgatca ggcaggagcc tcttcgacag
     2821 aagaaaattt gttcgtcgaa accccatgcc gcctaccttg ctcgaagcgc gggagatttc
     2881 caaatcgttt cctggaacgc gggcactgga ccgcgtgcac ttcgaagtgt tgccaggtga
     2941 ggtccatgcg ctggtagggg agaatggcgc cggcaagacg acgttgatga tgatcctggc
     3001 cggcgtcctc cagcccgacg aaggggcgat cttgtgggac ggtgtcgcgg tgccgctcac
     3061 cgatccccgc gcggctcagg gactcgggat cggaacagtc tttcaggaat tgagcctggt
     3121 gccggggctg agcatcgggg aaaacatctt cctcactcgg gctccggccc ggaccgcact
     3181 cggtctcgtc gaatggtctc gcgtctatgc tgacgctcaa gtggcgcttg cccagttggg
     3241 gatcaacgtc gatccgatgc tgcccgtgaa tgctcttgcg atgggcgcgc gccagatggt
     3301 ggagatagcc aaagcgctct ccctgcggtg tcgtatgctg ttgctggacg agccgacgtc
     3361 cgcgctatca ccgggcgagg tggaccggct cttcaccgcg atacgcgctc tccgggatca
     3421 agggctggga atcgtattca tcacgcatcg catcgcggaa gtattccaga ttgccgatcg
     3481 tgtgacggtt ctacgggatg gccatacagt ggcgacgctc gagatgtctg gcacaacccc
     3541 tgacgaggtt gtccactata tggtgggccg caacctgggg aagtccatgc ggcctccgcc
     3601 gccctcgacc tcaacgccga ttctcgaggt atcgggcctc tcgcggcccc cgatcctaca
     3661 aggcatcagt ttttcattgt atgcgggcga gatcctcggt gtcgcaggtc ttcccgattc
     3721 aggacgcgag gagctcggac gcactctgtt cggtatcgag ccgcctctca gcggtgcgat
     3781 ccgcgtggaa ggacgtgctc tctcgatccg gtctcctcaa gacgcgctgc gcgccggcat
     3841 tggttatctc ccgccggacc gccggacgct cggcctcttc ctccggatgc cggtcaaaga
     3901 caacatcgtg gttacagcat tggatcaaat cacgcactct ggaatcgtcg atgatggacg
     3961 cgcggcggcc atcgccgccc ggtacgtcga tcagctgcag gtacactccc cgtcggtgtc
     4021 gcaagttgtg gggcgcctca gtggagggaa ccagcaaaaa gtagcccttt ccaagtggct
     4081 cttccggcgg ccccgagttc ttatcgccga cggaccgacg attggtgtgg acgttggagc
     4141 tcggatggcg attcatcaat tgctgtacca actggcagcg gaaggaatgt cgatcgtgct
     4201 gctgtccacg gatctgcccg aacttctcgg gttgagcaac cgcatcatgg tgatgaacgg
     4261 tggtcgcgtg gcgggggtgc tggccggcaa cgaagcgagt gaacaacggg tgataaccct
     4321 tgcagcggga caacgactcg gaccaaagaa tgtctgatgg accagaagcc atctgtcgca
     4381 tctgcgtctc gggcggggac tgccgtccgc ctccgttcgg agaaggcctc cttcgcccat
     4441 ctgctgcaaa tgcgagaggt tctcctcttc gccgtcatac tagcactttc gctcgccatc
     4501 tcgaggctga cgccgtactt tctgacctcg gggaatctca aggcgatgct catcggtatg
     4561 gtccccacgg cgatcatcgc ggtaggaacg gccattgtcc tcgtcggagg cggcctggat
     4621 ctctcggtcg ggtcagtcat ggccctcagt ggaactgtgg cgggactctt gatgctgact
     4681 ggcacgccga tctggctggc actcagcctc acactgatgg tgggtggtct catcggcctt
     4741 gccaacggtt tagtgatcac ccggatccgt gtgaacc
//
LOCUS       DBNGSB010000174         5070 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3189670, whole genome shotgun sequence.
ACCESSION   DBNGSB010000174 DBNGSB010000000
VERSION     DBNGSB010000174.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 5070)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5070)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5070
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3189670"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="AC159B_05030"
     CDS             <1..192
                     /locus_tag="AC159B_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3377143.1"
                     /translation="RRLAGVAAAMVRALANVPLKLLENGRRDPTVWMEPVCDLVWRMG
                     EVAGMLAWYRAQMPTRMRS"
     gene            189..1115
                     /locus_tag="AC159B_05035"
     CDS             189..1115
                     /locus_tag="AC159B_05035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3377144.1"
                     /translation="MNTVSVLIATKDRAEHLRECLESVLLQDLQPSEVIIVDQSARPQ
                     DEAVTKLFAGSSVRLRYDYAPTLAGLTHARNRAIDQATSSILLFLDDDVILDSGYLRA
                     LLRVFDGDPHERVGGAGGVITNLSQTISAVQRVRSWLFYHGPFSVERDALAFQLRPGT
                     RSTRAPRLYGSNMAFRRRVVETIQFDESYTGYSFGEDRDFSVLAARQFELRWVPRATL
                     IHKETPRARLDRERFCELRVLSWLRFYRLHAPPTFMMRLSYVWLNAGFAVLLLKVWDL
                     PTVRGTLRGLRRLVAIQFGRARLLEALSEGWR"
     gene            1112..2236
                     /locus_tag="AC159B_05040"
     CDS             1112..2236
                     /locus_tag="AC159B_05040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3377145.1"
                     /translation="MKVLHVINTAEVGGGAEHLILLAQGLVGHGWRSTVVVGRDGPAG
                     ARLAAAGVPVQRLGPMGATTLLSLPRVLRAQRPDLVHLHGSRAGLLGTLSARWAGVRP
                     VVYTAHALAFRREDYPILRKVFVLAERTTCALADHVIYLTEGDLSAARKAGITTSHAT
                     VIPNGVDLARFTVDGDRKALGIAQNVPVIGMVARLVPQKDPMTFLDAARLVADVFPHA
                     KFLLIGDGPLRPRVEAAVRARGLEGQCLVLGTRQDVPQLLQAMDVVLLTSRWEGLALA
                     ALEAMAAAKPLVASRLPGLSAVIEDGLTGVLVRAGDAPEFARAAIALISDASRRTAMG
                     TRGQRRVAEHFSLEGMVASTERVYRSALAVSGSPADLRTT"
     gene            2410..3843
                     /locus_tag="AC159B_05045"
     CDS             2410..3843
                     /locus_tag="AC159B_05045"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR3377146.1"
                     /translation="MAGGNGQMLTEGRRRQLRALLLIVLDASMIAAAFSLAYLFRFYW
                     EIVPALDEPPVTPYFAVLTPLIPAWILVFAAYGLYRDRIPSHLDEALRITSAVAVGMM
                     ALLAASFFYREFSYSRLWILYAWVLSTILLLTARLIMRAAHRLLRMRGIDVRRVLVVG
                     AGQEGRMVVEVLRTRPELGQRPVAFLDPDQQSEVDGLPVAGRPDAVLQTVRRFGAHHV
                     IIALPPDAREVAMRVAAICQEAGVPFAIVPDLYTLVAAGAEVEIVERLPLITPRGSRT
                     GGWGRRVKDASDVAGALLGLLITLPLLVVIALLIVCDSPGPVFFRHVRIGRAGVPFLA
                     WKFRTRRDGADEQLSADSRLRAQYQRSFKLVEDPRVTRIGRWLRRTSLDELPQLINVL
                     KGEMSLVGPRPIVEDELRKYGPWERRLLCVKPGLTGLWQVLRHDEPNYSQRVRLDMYY
                     IDHWSVGLDVKILLNTLPSVITGRGAY"
     gene            3845..4963
                     /locus_tag="AC159B_05050"
     CDS             3845..4963
                     /locus_tag="AC159B_05050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3377147.1"
                     /translation="MRVALVHDWLITLGGADRVLLALHELFPEAPVHVSLFDPSRLPE
                     EFHRLKIHPSWLQRIPRATKHHRWLVPVMPVVFHTMNVDGAQVIISSSHACAKGVRKP
                     KGAVHICYCHTPMRYAWEFEPGYLDALPAVLRPAARTAMLGLRWWDRATSDGVDHFIA
                     NSKFVAGRIRRHYGREATVIYPPVETEYFTPSGRPGGSYLVVSRLVPYKRVDLAVEAF
                     NRIGRPLVVVGDGPEASKLRAKARPNITFVGEVPDAELRAHYRRCAALIFPGEEDFGL
                     VPLEAQACGRPVVAFGRGGALESVVDGMSGGFFFEPTADALMAAVRAFDQTSYDPAAI
                     RTHAEQFSRQRFLQQIGAFVESVMTDANAQTRKRAIPA"
BASE COUNT          836 a         1598 c         1656 g          980 t
ORIGIN      
        1 cggcgcctcg ccggcgtcgc cgctgccatg gtccgcgcat tggcgaacgt gccgctcaag
       61 ctgctcgaga acggacgtcg cgatcccacg gtctggatgg agccggtctg cgatctggtg
      121 tggcggatgg gcgaagtggc ggggatgctt gcctggtacc gggcacaaat gccgacccgc
      181 atgaggtcat gaacactgtc tcggtgctca ttgccaccaa agatcgcgcc gagcacctgc
      241 gcgaatgctt ggagtccgtg ctcttacagg atctccaacc cagtgaagtg atcatcgttg
      301 atcagagcgc tcgtccgcag gacgaggcgg taaccaaact atttgcgggc agtagcgtcc
      361 gcctgcgtta tgattacgct cccacgttgg ccggacttac acacgcccgc aaccgtgcca
      421 tcgatcaggc gacctccagc atcctcctct ttctcgacga tgatgtgatc cttgattcgg
      481 ggtatctccg cgccttgctg agggtattcg acggcgatcc gcacgagcgg gtcggcggcg
      541 ccggtggcgt gatcacgaat ctgtcgcaga cgatctccgc ggtgcagcgg gtgcgctcct
      601 ggctcttcta ccacgggccg ttttcagtgg agcgggatgc gctggcgttc cagttgcgtc
      661 ctggcacacg ctcgacgcgg gccccgcgtc tttacggttc caacatggcc ttccggcgcc
      721 gcgtcgtgga gaccatccag tttgacgaat cgtacaccgg gtacagcttt ggcgaggatc
      781 gagacttctc cgtgctcgcc gccaggcaat tcgagttgcg ctgggtgccc cgggccacgc
      841 tcatccacaa agaaacgccc cgcgcgagac ttgaccgcga gcggttttgc gagctgcggg
      901 tgttgagctg gctgcgcttc taccggctcc atgcgccgcc gacattcatg atgcgactct
      961 cgtacgtgtg gctgaatgcc ggcttcgcgg tcctgctgct caaagtgtgg gatctgccga
     1021 ctgtgcgggg aacgctccgc ggactgcggc ggctcgttgc catccaattc ggaagagcac
     1081 gattactcga agcgttgagc gaaggctgga gatgaaagtt ctccacgtca tcaacacggc
     1141 agaggttgga ggaggcgccg agcacctgat cctgctcgca caggggctgg tggggcatgg
     1201 ctggaggagc acggtcgtcg tgggacgcga cggacccgcg ggcgcgaggc ttgccgccgc
     1261 gggtgtgccg gtgcagaggc tcggccccat gggcgccacg accctcttgt cactcccccg
     1321 tgtgctccgc gcgcagcgtc cagacctcgt ccacctgcac ggaagccggg cggggttact
     1381 ggggacgttg agcgcgcgct gggccggcgt tcggccggta gtgtacacgg cccatgcctt
     1441 ggcgttccga cgcgaggact accctatcct tcgaaaggtt ttcgtcctgg ccgagcgtac
     1501 gacatgcgcc ctcgccgacc acgtcatcta cctgacagag ggagacctgt ctgcggcaag
     1561 aaaggccggc atcacgacat cccacgcgac cgtcattcct aatggcgttg atctggcccg
     1621 gttcaccgtc gacggtgacc ggaaagcgct cggtatcgct cagaacgttc cggtgatcgg
     1681 catggtcgct cggttggtcc ctcagaaaga tcccatgacg tttctggatg ctgcgaggct
     1741 cgtggccgac gtgtttcccc acgcgaagtt cctgctgatt ggagacgggc ccctacgccc
     1801 gcgcgtcgag gccgcggtgc gcgcgcgtgg gctggaggga cagtgcctgg tgctcggcac
     1861 gcggcaggat gtgccgcaac tgcttcaggc tatggatgtc gtgctgctca cgtcgcgctg
     1921 ggaagggctt gcgctcgcgg cgctcgaggc gatggccgcc gcaaaaccgc tcgtcgcctc
     1981 acggctcccg ggcttgagcg cagtcatcga agacggcctg accggagtcc tggtcagggc
     2041 gggcgacgcg ccggaattcg ctcgagcggc gatcgcgctg atttctgatg cgtcccggcg
     2101 cacggcaatg ggaacgcgag gacagcgaag ggtcgccgag cacttttctc tggaaggcat
     2161 ggtcgcttcg acggagcggg tgtaccgctc tgcgctggcg gtttcgggat cgccggccga
     2221 cttacggact acttaacccg gcaccaagca aaattccccc ggcctcgctg tagctcggct
     2281 ccagactcaa tcaacgtgca aaattcaccg acctcgctac tggctcggtc tcctcaacag
     2341 ctccgcccgc ctgctgcgca ggcagcatcg gcggacgcca cctgactatc tgaaaaacgc
     2401 cgggggcata tggctggtgg gaacggccag atgctcaccg aaggacggcg gcgacaactc
     2461 cgagcactgc tgttgatcgt gctggatgcc agcatgatcg cggcggcatt ttcgctggcg
     2521 tatctcttcc ggttctactg ggagatcgtc cccgcactcg atgagccgcc ggtgacgcct
     2581 tactttgcgg ttctgactcc gctcatcccg gcgtggatcc ttgtgttcgc cgcctacggc
     2641 ctgtaccgcg accggatacc ctcgcatctc gacgaagcgc tgaggataac aagcgcggtc
     2701 gcagtcggca tgatggcgct cctcgcggca tcgttcttct accgcgagtt ttcgtactca
     2761 cggctatgga ttctctacgc ctgggtattg agcaccatcc tgcttttgac cgcaagactc
     2821 atcatgcgcg cggcgcatcg cctcctgcgc atgcggggga ttgatgtgcg cagggtcctc
     2881 gtcgtcgggg cagggcagga gggccggatg gttgtggagg tgctgcggac tcgacctgaa
     2941 ctggggcaaa ggcccgtggc atttctcgat cctgaccagc aatcagaggt tgacgggctg
     3001 ccggtggccg gcagaccgga cgcggtgctc cagacggtcc gacgtttcgg ggctcaccat
     3061 gtgatcattg cgctgccgcc cgatgcgcga gaggttgcga tgcgggtggc ggcaatctgc
     3121 caggaggcag gcgtaccctt cgccatcgtg cccgacctct acacgcttgt ggctgccggc
     3181 gccgaggtcg agatcgtcga gcgtcttcct cttattactc cgcgaggcag ccgcacgggc
     3241 ggatgggggc gccgcgtgaa ggacgcctcc gatgtcgccg gtgcgctgct cgggctgctc
     3301 atcacgctgc cgctgctcgt tgtcatcgct ctgctcatcg tatgtgacag cccagggccg
     3361 gtcttcttcc gccacgtgcg tatcggcagg gccggcgtcc cgttcctcgc ctggaagttt
     3421 cgcacaaggc gtgacggcgc ggatgagcag ctctccgcgg attcgcggct gcgcgcccaa
     3481 taccagcgct cgttcaagct ggtcgaagat cctcgcgtga cccgcatcgg acggtggctc
     3541 cggcgcacga gtctggatga gctgccgcag ctgatcaatg tcttgaaggg cgagatgagt
     3601 ctggtcggcc cgcgcccgat cgtggaagac gaattgcgca agtacggtcc gtgggagcgc
     3661 cgcctgttgt gtgtcaaacc cggtctcacc gggttgtggc aggtgctgcg ccacgacgaa
     3721 cccaactaca gccagcgcgt gcgtctggat atgtactaca tcgaccactg gtcggtcgga
     3781 ctcgacgtga agatcctgct gaacaccctg ccctctgtga ttaccggcag aggggcgtac
     3841 tgaggtgcgg gtcgccctcg tccacgactg gctgatcaca ctcggaggcg cggaccgggt
     3901 cctcctcgcg ctgcacgaac tgtttccgga ggcgcccgtt cacgtcagcc tcttcgatcc
     3961 ctctcgtctg ccggaagagt ttcaccggct caagatccac ccaagctggc tccaacgcat
     4021 tccccgtgca acgaagcacc accggtggct cgtcccggtg atgccggtcg tattccacac
     4081 gatgaatgtg gatggcgctc aggtcatcat tagcagcagc cacgcgtgcg ccaaaggcgt
     4141 ccgaaaaccg aagggcgcgg tgcacatctg ctactgccat acgccaatgc gctacgcctg
     4201 ggagttcgaa cccgggtacc tggatgcgtt accggcggtg ctccgccccg cggcgcgaac
     4261 ggcaatgctt gggctgcgct ggtgggaccg agcgacctct gacggagtgg accacttcat
     4321 cgccaactcc aagttcgttg cgggccggat tcggagacat tacgggcgcg aggccaccgt
     4381 gatctaccct ccggtggaga ccgagtattt cacgccatca ggccgtcccg gagggtcgta
     4441 tctggtcgtc tcacgcctgg tgccgtacaa gcgcgtcgat ctggcggtcg aggcgttcaa
     4501 cagaatcggc cggcccctcg tcgtggtggg cgatggaccg gaggcatcga aacttcgggc
     4561 aaaggcgcgg ccgaacatca cgtttgtggg agaagtcccc gacgcagagt tgcgggcgca
     4621 ttaccggcga tgtgccgcac tcatctttcc cggagaagaa gatttcggat tggtgcctct
     4681 ggaagcgcag gcctgtggtc gccccgtcgt cgcgttcggc cgcggcggag cgctcgagag
     4741 cgtcgtcgac gggatgagcg gagggttttt cttcgagccc accgccgacg cgcttatggc
     4801 cgcggtgcgg gcgttcgatc aaacatcgta tgatcccgcg gcgattcgga cgcatgcgga
     4861 acagttttct cgccagcgat ttctccagca gattggagcg ttcgtcgaaa gcgtcatgac
     4921 ggacgcaaac gcgcaaacgc gcaagcgcgc aatcccggca tgatacacgc gcctcgaagt
     4981 cctgagattg cgaatgtgac tcgggaggtc gaggcgctcc tcgcgaacct cgagcgggtc
     5041 atcatcggca aacggcctgt gatcgatctc
//
LOCUS       DBNGSB010000175         3590 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2118537, whole genome shotgun sequence.
ACCESSION   DBNGSB010000175 DBNGSB010000000
VERSION     DBNGSB010000175.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2118537"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2040)
                     /locus_tag="AC159B_05055"
     CDS             complement(<1..2040)
                     /locus_tag="AC159B_05055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3377148.1"
                     /translation="MPAVGNSVARKDGIGKATGGAQYADDITFPGMIHGRTIRSTIPR
                     GRVKSIRFDFDTAGFTVVDYRDIIGRNSVDLIEKDQPFLVEQEVRHMAEPILLLAHED
                     RERLNAANIAIEYEEEKPLFDPGQSSEVFKKIDIVKGDVASALAAADLVVEGTYRTRH
                     QEHVYIEPNGVIAVPEEVEGRRGVAIFGSVQCPFYVIKALRCLFGEHPPIRVIQTETG
                     GGFGGKEEYPSMIAGHAALLALKSGRPVKIIYDRAEDMVATTKRHPSIVRHRTGVMRD
                     GRIVAMDIDVLMDGGAYVTLSPVVLSRGCIHAAGPYRCDNVSIRGRTVFTNTPPNGAF
                     RGFGAPQTQFAVEVHMERVAEALGMDSVALRRINALRPGDTTATGQTLREDTSALMVL
                     DEAVRRTDFLRKRERYKGTNRALGLSLFYHGAGFTGSGELHLKSRARLELTPTGVRIA
                     TGSTEIGQGTRTTHAQIVSDALGIPYEDVEVAQPDTARVADSGPTVASRTCMVVGKIL
                     EKCALEMKERLNGLSPAEYHARHGAFSVEQMYEPPDWIKWDDAAYRGDAYATYGWGCD
                     VVEIEVDPDTFEVRPVKLTAVQEFGRPIHPALARGQIEGGTAQGLGYALLERVVMRNG
                     AMANSQLTNYTIPTTLDMPELDVVMLENPYPGGPFGAKGLGELPMDGPAPAVVNAL"
     gene            2913..3377
                     /locus_tag="AC159B_05060"
     CDS             2913..3377
                     /locus_tag="AC159B_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3377149.1"
                     /translation="MTTYQLSVNGQRREVDVDPTTPLLWVLREDLKMTGTKYGCGISA
                     CGACTVHLDGIAARSCVVPVSLIGNRPIVTIEGIGKDPVGRAVQDAWVEKDVVQCGYC
                     QSGQIMTAVALLKDKPRPTDANIEQSMGGNLCRCGTYVRIREAIKSASTQLR"
     gene            3390..>3590
                     /locus_tag="AC159B_05065"
     CDS             3390..>3590
                     /locus_tag="AC159B_05065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HIR3377150.1"
                     /translation="MRPLLKTESDWRLSRRSFLRVSAAAAGGLLVSVYVDLPAFAQEG
                     NQALPQKVYPPDAFVHIRPDGQI"
BASE COUNT          681 a         1116 c         1100 g          693 t
ORIGIN      
        1 aagtgcgttg actacggcgg gcgccggacc gtccattggg agctcgccga gccccttcgc
       61 gccaaacgga ccgccggggt agggattctc cagcatcacc acatcgagct ccggcatgtc
      121 gagggtcgtc ggaatcgtgt agttggtgag ctgcgagttg gccatcgccc cgttgcgcat
      181 aaccacgcgc tcaagcaacg cgtaaccaag gccctgtgcc gttccgccct cgatttggcc
      241 ccgcgctagc gccggatgaa ttggccgccc gaactcctgc actgcagtca gcttcaccgg
      301 ccgaacttcg aacgtgtccg gatcgacttc gatctcgacg acatcgcatc cccagccgta
      361 cgtcgcgtaa gcgtcaccgc ggtaggccgc atcgtcccac ttgatccagt cgggaggctc
      421 gtacatctgc tcgacggaaa atgcgccgtg gcgcgcgtgg tattccgcgg gcgacaaccc
      481 gttcaggcgc tccttcatct cgagcgcgca tttttcgagg atcttcccca ccaccatgca
      541 cgtgcgcgag gcaacggtgg gaccgctgtc ggcaacacgc gccgtatccg gctgtgcgac
      601 ctctacatct tcgtaaggga tgccgagcgc gtccgacacg atctgcgcgt gggtagtacg
      661 cgtgccctga ccgatttccg tgctgccggt tgcgatccgc acaccggtgg gcgtcagctc
      721 gagcctcgcc ctcgatttca ggtgcagctc accactgccc gtgaagccgg cgccgtgata
      781 gaacaatgac agacccaacg cgcggttcgt ccccttgtac cgctcgcgtt tgcgcaggaa
      841 gtccgtgcgg cgcacggcct cgtcgagcac catgagtgcg ctggtatcct cacgcagcgt
      901 ctgtccggta gcggtggtgt caccgggacg cagtgcattg atcctgcgca gcgcgaccga
      961 gtccatgcca agcgcctcgg caacgcgctc catgtgcacc tcgacggcaa actgagtctg
     1021 cggcgcgccg aagcctcgga acgcaccgtt gggcggagtg ttggtgaaca ccgtccgccc
     1081 gcgaatgctg acgttgtcgc aacggtacgg accagcagca tggatacatc cgcgtgagag
     1141 caccaccggg ctcagcgtga cataggcgcc gccgtccatg agaacatcga tgtccatcgc
     1201 cacgatgcgg ccatcgcgca ttaccccggt gcgatgccgc acgattgaag ggtgacgctt
     1261 ggtcgtggcg accatgtcct ccgcacggtc gtagataatc ttgaccggcc ggcctgattt
     1321 gagcgcgagc agcgcagcgt gtcccgctat catcgacggg tattcttcct tgccgccgaa
     1381 cccgcctccc gtttcagtct gtatgacgcg gatgggaggg tgctcgccga acaggcacct
     1441 cagcgccttg atgacataga acgggcactg caccgagccg aagattgcga ctcctctacg
     1501 gccctcgact tcttccggaa cagcgatgac gccgttcggc tctatataga cgtgctcctg
     1561 gtgcctggta cggtacgtcc cctcgaccac gaggtcagct gccgcgagag cgctggctac
     1621 atctcccttc acgatgtcga tcttcttgaa tacttcagac gattgcccgg gatcaaatag
     1681 tggcttctcc tcttcgtact cgatcgcgat gttcgcggcg ttgagtcgct cccgatcctc
     1741 gtgcgcgagc agcagaatgg gctccgccat gtggcgaacc tcctgctcga cgagaaatgg
     1801 ctggtccttc tcgatcagat ccacggagtt tcgtccgatg atgtcgcggt agtcgacgac
     1861 cgtgaaacca gccgtgtcga agtcgaagcg gatggacttt acgcgaccgc gcggaatggt
     1921 cgagcgaatt gtgcggccgt ggatcatgcc ggggaaagtg atgtcgtccg cgtactgcgc
     1981 gcctccggtg gcctttccga ttccgtcctt gcgggcgacg ctgttaccga ctgctggcat
     2041 gctcgatcgc gacctccatg actgctgatt gctgagccgg aacatgcgac gctgcccggc
     2101 gggaagcaag attcgtgacc taggctagcg cacgacagca acctgcgcgc tatcgacctc
     2161 ttgacgaaac aaaacttggt gtgtagcgtg ccgctgacaa cacaagacgt cgtgtattgc
     2221 gcgctccgga gagaggcacc agtggcgcgc tgtaaaggag gcacggatgt tggccaaccg
     2281 ctcgtttgta ctccgggccc ttcttgtgac gtctatcgcc tttgcgggtt ggacagcatt
     2341 ggcatccgtc gaaatagcgg acggcaaaac ccccacagct gatgccaagc ccacatccgg
     2401 cttagcggca tgggacgacg tctattccgt cttgacctat ccgcgctgca tcaactgtca
     2461 caccgccacc tcctatccgc agcagggaga cgatcgccag cgtcacttcg ccaacgtcat
     2521 ccggggccct gaaggcaagg gtgtagccgg actgaattgc gcgagttgcc atcaggagaa
     2581 gaattccgac gccactggag tgccgggcgg acacaactgg catttggcgc cactctccat
     2641 gcgctggcaa gacttgaacg atcggccttt gtcgagcgcg gaggtgtgtc gtgcgctcat
     2701 tgatcgcgag aagaatcatg gtctcgatgg acctggtttg ttgaagcacc acgaggaaga
     2761 gccgctcgtt cactgggcct ggaatccggg gcgacggcca gacggcacta cacggtcgct
     2821 cccgccactc acgcatgccc agtttgtaga cgcaactcgc cgctgggttg ctgcaggcac
     2881 gccctgcccg aaaggaccgt agaggatcag tgatgacgac ttaccaactc agcgtcaacg
     2941 gccagcgtag agaggtagac gtcgatccga cgacaccatt gctgtgggtt ttgcgggaag
     3001 acctcaagat gacgggcacg aaatacggtt gcgggatcag cgcctgcggc gcgtgtaccg
     3061 tccacctcga cggcatagcg gcacgctctt gcgtcgtacc cgtctcgctg attggcaatc
     3121 gtccgatcgt cacgatcgag ggaatcggaa aggatccggt gggccgcgcg gtgcaggatg
     3181 cctgggtgga aaaggacgtc gtgcagtgcg gatattgtca gagtggacag atcatgaccg
     3241 cggtcgcgct gctgaaggac aagccgagac cgactgatgc gaacatcgaa caatcgatgg
     3301 gtggaaatct ctgccggtgc ggcacctatg tgcggatccg tgaagcgatc aagagtgctt
     3361 caacgcagct gaggtaacag acgcggacga tgcgaccact actcaaaacg gaaagcgatt
     3421 ggcgcctgag ccggcgaagc ttcctgcgtg tatccgccgc cgccgccggg gggttgctgg
     3481 tgtccgtcta tgttgatctg cccgcgttcg cgcaggaagg gaatcaagcg ctgccgcaga
     3541 aagtctatcc gcccgatgcg tttgttcaca tcaggcctga cggtcagatc
//
LOCUS       DBNGSB010000176         3030 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3498563, whole genome shotgun sequence.
ACCESSION   DBNGSB010000176 DBNGSB010000000
VERSION     DBNGSB010000176.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3498563"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="AC159B_05070"
     CDS             complement(<1..481)
                     /locus_tag="AC159B_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HIR3377151.1"
                     /translation="MISETFRDHIKFLITYLRPQWQRAAMLAVVLLVSIGLQLVGPQI
                     LARFIDMARAGAAIAELMQVAAAFLAVACAGQIISAIAVYASENVGWTATNLVRADLA
                     LHCLRLDLSFHNAKTPGEMIERVDGDVTHLSRFFSQFAVRVFGNLLLLLGVLVMIFRV
                     "
     gene            complement(526..1134)
                     /locus_tag="AC159B_05075"
     CDS             complement(526..1134)
                     /locus_tag="AC159B_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018033870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HIR3377152.1"
                     /translation="MQLDTETIRRERTLVFLKPDGVQRGLVGEVVRRFENAGLKLIGM
                     KMVRPNRKLLDRHYPTDAGFLTTIGGKTKDAFATYGLDVKRETGTDDPQEIGRQVRGW
                     LIDYVSSGPVVAFVLEGIHAVSAVRKLVGDTLPYRAAPGTIRGDYSIDSPTVANLAKR
                     PGRNLIHASGSTEEADVEIALWFEESELFEYDRSDAKAMFGA"
     gene            complement(1118..2764)
                     /gene="ggt"
                     /locus_tag="AC159B_05080"
     CDS             complement(1118..2764)
                     /gene="ggt"
                     /locus_tag="AC159B_05080"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione gamma-glutamate
                     hydrolase [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIR3377153.1"
                     /translation="MENVTPTPQGHQGRAVVIARNGIVAAGHPLAAAAGLHVLQGGGN
                     AVDAAVAVAGVLGVVQPMMSGLGGDAFILIWSAAERKVHAVNGSGIAPFAATREWFTS
                     RGYSKMPLRGMLSVSVPGAVDAMQTILGKWGSGRYTLSRLFEPAIEYADRGFPVAPKV
                     AYWIGQAAEVLGKFPSSARVFLPQGRPPHPGEILINRDYAASLRAVAAGGADAFYRGQ
                     VSRRISDYVRGHGGLLTEREFAEHRSEVHEPIRTTYRGLTVCTTAPPSQGVIVLEILN
                     ILEGFAPEQLQWGTAEAVHLAVETKKLAVADRLAYLGDPKFVDNPLEVLLAKDYATRR
                     RGAVDRQRAQSEAPAGALPEAVGDTTYFCVADREGNVVSYITSLSASFGCGEVVDGTG
                     IMLNNRAGRGFVLQDDHPNCVAPGKRTMHTLMPFMALKEGTPHLAWGTPGGDGQPQWN
                     TQVFLNIVDGRMNIQQAIDAPRWFSFPSTDPASLPGPFELRMESGFPEQTRSRLTERG
                     HVLREMGEMESGGGCQTILISDGVYQGGSDPRVDGCAIGY"
     gene            2816..>3030
                     /locus_tag="AC159B_05085"
     CDS             2816..>3030
                     /locus_tag="AC159B_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3377154.1"
                     /translation="MIPRTIAAVLFVLFLTSPSYAQVTVADDVTEDALSWNSQPKLAR
                     APDGSIYLALVKRAGGVSQIFLASSPDG"
BASE COUNT          573 a         1011 c          893 g          553 t
ORIGIN      
        1 ccacgcggaa gatcatcacc aggacgccga gcagaaggag caggttgcca aacacccgga
       61 cggcgaactg cgagaaaaac ctggacagat gcgtgacgtc accgtcaacc cgctcaatca
      121 tctcgcctgg cgtcttcgca ttgtggaacg ataggtccag ccgcaggcag tgcagggcca
      181 ggtcggcgcg cacaaggttg gtcgccgtcc aaccgacgtt ctcgctggca tacacggcaa
      241 tggcgctaat gatctggccg gcgcacgcca ccgcgaggaa ggcggcggcg acctgcatca
      301 gctcggcgat cgccgctccc gcccgcgcca tgtcgatgaa gcgcgcgagg atctgaggac
      361 cgaccagctg caggccgata ctgaccagca ggacgaccgc cagcatggcc gccctctgcc
      421 actgcggccg cagatatgtg ataaggaatt tgatgtgatc gcgaaatgtc tcgctgatca
      481 tactgcgctc aaggatgagg gcttgggctc aggacggcgg agcgattatg cgccaaacat
      541 ggctttggcg tcggatcgat catattcgaa gagctctgat tcctcaaacc acagggcgat
      601 ctcgacgtct gcttcttcgg ttgaccccga cgcatggatc aggttccgcc cggggcgctt
      661 cgcaagattt gcaacggtgg gagaatcgat cgagtaatct ccgcggatgg tccccggcgc
      721 cgcgcggtac ggcaaggtgt ccccgacgag cttacgcacg gcgctcactg catggatgcc
      781 ttccagcaca aacgcgacga ctgggcccga cgacacatag tcgatgagcc acccgcgcac
      841 ctgacggccg atctcctgtg gatcgtcggt ccccgtttct ctctttacgt cgagtccgta
      901 tgtggcaaac gcgtctttgg tcttcccccc gatggtcgtg agaaaccctg catcggtggg
      961 ataatgccgg tccagcaact tcctgttcgg ccggaccatc ttcattccga tgagtttcaa
     1021 cccggcattc tcgaatcgcc gcacgacctc cccgaccaat ccccgctgga ccccatcggg
     1081 cttcaagaag acgagagtgc gttcccggcg gatcgtttca gtatccaatt gcacacccgt
     1141 ctactcgtgg atccgatccc ccctggtaca cgccatcgct gatgaggatg gtctggcagc
     1201 cgcctccgga ttccatctca cccatttctc tgaggacgtg cccgcgttca gtgagacgag
     1261 aacgcgtctg ctccgggaat ccagattcca tccgtaattc gaacggcccg ggcagactcg
     1321 caggatcggt gctggggaag ctgaaccagc gcggcgcatc aatcgcctgc tggatattca
     1381 tccgaccgtc cacgatgttt agaaacacct gcgtgttcca ttgcggctgc ccgtccccac
     1441 cgggcgttcc ccacgccagg tggggtgtgc cttccttcag ggccatgaag ggcatcaacg
     1501 tgtgcatcgt acgcttgccc ggcgcaacac agttcggatg gtcatcctgg agcacgaacc
     1561 cgcggccggc gcggttgttg agcatgatgc cggtaccatc gacaacttcc ccgcagccga
     1621 agctggccga gaggcttgtg atgtacgaaa cgacgttccc ttcccgatcg gcgacacaga
     1681 aataggtggt gtccccaacc gcctccggca gtgcgccggc gggcgcctcg gactgcgccc
     1741 gctgccggtc gacagccccg cggcgccggg ttgcatagtc ttttgccagc agcacttcga
     1801 gcgggttgtc gacaaatttc ggatccccaa gatatgccag acggtcggcg acggcgagct
     1861 tcttcgtttc caccgcaagg tgcacggcct cggcggttcc ccactgcaac tgctcaggag
     1921 cgaacccttc caggatgttc agtatctcga gcacgatgac accctgggac ggcggtgccg
     1981 tcgtgcagac cgtcagccca cggtacgtcg tgcgaatcgg ctcatgcacc tccgaacgat
     2041 gctccgcaaa ctcacgttcg gtcagcagtc ctccgtgccc tcgcacgtag tccgaaatgc
     2101 gcctgctcac ctggccacgg taaaatgcat ccgcgccgcc ggcggcgacg gcccgcaggc
     2161 tggccgcgta atccctattg atcaagatct ctcccgggtg gggcgggcgg ccttgcggta
     2221 agaagacgcg cgccgagctc ggaaatttcc ccagcacctc agccgcctgg ccaatccaat
     2281 acgctacctt gggcgcgacc ggaaatccgc ggtctgcata ctcgatggcc ggttcgaaca
     2341 agcgcgaaag agtgtaccgg cccgagcccc atttgcccag gatcgtctgc atggcgtcga
     2401 cggcaccggg caccgacacg gacagcatcc ctcggagggg catcttcgaa tacccccgcg
     2461 acgtaaacca ttcgcgcgtc gccgcgaagg gggcgatgcc gctgccattc accgcatgaa
     2521 ctttccgctc agccgcggac cagatcagga tgaacgcatc gccgccgagt cccgacatca
     2581 tcggctgcac gacacccagg actccggcca cggcgacggc cgcgtccacg gcattcccgc
     2641 cgccctgcag aacgtggagg cccgccgcag cggccagcgg gtgtcctgcg gcgacgatgc
     2701 cattccgcgc aattaccacc gcgcgtcctt gatggccctg cggtgtggga gtcacgttct
     2761 ccatgattgg ggcatgctgc agggaagtcc tcctacggac ataagtgtac gagacgtgat
     2821 cccccgcaca atcgccgcgg tactgttcgt tttgttcttg acctctccct catatgctca
     2881 ggtgacggta gccgatgacg tgacagaaga cgcgctgtcg tggaacagcc agccaaaact
     2941 tgcccgcgcg ccggacggct cgatctacct tgccctggtc aagcgcgccg gcggagtgtc
     3001 ccagatcttt ctggcgagct cccccgatgg
//
LOCUS       DBNGSB010000177         7537 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_586577, whole genome shotgun sequence.
ACCESSION   DBNGSB010000177 DBNGSB010000000
VERSION     DBNGSB010000177.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7537)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_586577"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="AC159B_05090"
     CDS             <1..375
                     /locus_tag="AC159B_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020519239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HIR3377155.1"
                     /translation="EIGTPSGRFDPPTPEQMDAALLKGTGASPGKATGTARVIVGPEE
                     FVRVRPGDILVCPSSNPSWVLLFGIIRGLVTNTGGVLSHAAVVAREFGVPAVVGTGEA
                     TRRISDGQSIEVDGTAGEVRIL"
     gene            372..1571
                     /locus_tag="AC159B_05095"
     CDS             372..1571
                     /locus_tag="AC159B_05095"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3377156.1"
                     /translation="MTAAIIAIAAGAILVPLNSTMLSVALPAIMADFSVTASTVSWLV
                     TAYLATVAVAMTVSGNAGDRLGHRRMFLAGVAGFAASSLLGTVAWSFPILVVSRVLQA
                     ASGASITTNSIGLIRALSPEHRRGASFGMFEMLILTSAAAGPFVGGLLTSAFGWRSLF
                     VIAVPIATFSALLFKAVIPLQERREALAPVDIGTLLRQSLGLFRVRAFAAAVAAVFGS
                     TVILHTSMILIPIFTQSLLRASAAVSGIVMFVYFGTSALISPMGGRLSDNAGRRFPAV
                     AGMICTAAGLAALSRWAGSVTIPQVAVFLSLMGLGSGLSGTPRQTSAIESVPPSATGM
                     AAGTYFTMRYLGGVVGATLAGVMLREGVTTPAVARGFGLLALVACAVAVVSTGLTGRS
                     AVSHSRS"
     gene            1633..3027
                     /locus_tag="AC159B_05100"
     CDS             1633..3027
                     /locus_tag="AC159B_05100"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3377157.1"
                     /translation="MTKFTALEEFILEKMAETKLPGVSIALVDQGEVAYSRGFGFRDV
                     ERSLPATPRTLYGIGSVTKSFTCIALMQLQEQGKLRVDDPVDRYVPLTVRPMGDPVRI
                     HHFMSHSSGIPALAYAEAVIRHSVGASDSYVPIGSYEDMLNFVNGAEKWTHARPGERW
                     FYLNEGYVLLGAIIEKVSGRPYADYIHEHVIAPLQMERTSFTREDLQRETDAATPYVV
                     TKDKEQIPSRYLNGKLSSDGGLMSSVEEMARYVMMFLDGGRGPRGAIVSGDSLKAMMS
                     PHVPLPLEVYPAARPVPSGHYGYGLSMNPDFYGHTVVGHGGSVLVSTAHMAFIPDRRI
                     GAMVLANGSGYPLQYIAFSALATMLGEDPWEYPALRVERTLGQLTGSYETYQHTYPAT
                     VTRNGDFLLITIRNRHTEQIVPLIPEDLDPLRPRFFTLAGGRRLPVEFSVNNGDVELI
                     YERYKFRRTGRLTS"
     gene            complement(3266..4720)
                     /locus_tag="AC159B_05105"
     CDS             complement(3266..4720)
                     /locus_tag="AC159B_05105"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3377158.1"
                     /translation="MTVMQQAYQLIIDGHGIDALSGQTTTVTNPATGEVVASVSIAGE
                     EDVDRAVQAGRRAFEAGKWAQMSAGERGRVLVRIAGLLRERVESLARLEVLNSGKPIR
                     DARAEVLAAAECFEYYGGAATKWFGETIPVTDRGLDVTLREPIGVVGLIVPWNFPLMI
                     ASWKVAPALAVGNAAVLKPASATPLTALALGAIALEAGLPAGVLNVITGPGGTTGAAM
                     VRHPHLGKIAFTGETTTGIEIMKAAADTMTRVSLELGGKSPNVIFADADPERAIPGAV
                     SAVFANAGQDCCARSRLFIEHSIYERTLAGLVEQTEQLRTGDPLAEETQIGSLISLTH
                     RDRVRGYVDVGAREGATVLCGGRPPADPALARGAYLLPTVLGGVRNAMRVAQEEIFGP
                     VVCAIPFTDEAEAARMANDSPYGLSASIWTRDIGRALRMARAIKTGVISINSNHSVHQ
                     EAPFGGMKMSGFGRELGMHALELYTELKNIFIAE"
     gene            complement(4767..5552)
                     /locus_tag="AC159B_05110"
     CDS             complement(4767..5552)
                     /locus_tag="AC159B_05110"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HIR3377159.1"
                     /translation="MGTTRRLQDKVALITGAASGMGRVAATLFAAEGAKVVIADVTER
                     AGEDTAKEITSAGGDAVFVRADVSKAAEAEAMVRFAVQRFGRLNVLYNNAGIFHSEDG
                     GTVETSEATWDRVIDINLKGVWLGCKYGIPALLRSGGGSIINVASFVALMGAATPQIA
                     YTASKGGVLSITREIAVEYARKGIRANALCPGPVETPMLAELLADPQRRARRVVHIPI
                     GRFGQAEEIARAALWLASDESSLMTGAALVVDGGITAAYVTPE"
     gene            complement(5572..6837)
                     /locus_tag="AC159B_05115"
     CDS             complement(5572..6837)
                     /locus_tag="AC159B_05115"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3377160.1"
                     /translation="MRNGSWSSGFTGLCLGQFLAHQTSLTFSILIPILSSEWRLSSSQ
                     AGLTLGVFQLGTLAAYVAVGFLLDRVRSKPIMVWSGALVGLGDLLFAFGARDFASGLF
                     FRLLVGIVFGGLYLPALKQIAETIPRDRRGTATGIYIAVIVLAYAVPLYYVGLLAPRF
                     GWRWTMAGVGAVELLGALVMAWKVPSIPLPAMLKPLGPSRYVGDVLGNRPARRVIIGY
                     TGHNWELFGMWGWLTPFMVAMLLARGSVRGDALAWGGMLAAGSIGLGGAIGAVVGGRL
                     SDRLGRAEIATLMLGASLFCSLTFGWLFAAPLILVVGIALLYGVSSLADSPSYSAKLM
                     EVVPPHSLGGAFSLQMLTGWTATALAPLAVGIVLDMTKAAGLRPAAHWGWAFALLALG
                     PLAGLIAMIPLRRRSAALKALPQTGGSAD"
     gene            complement(6830..>7537)
                     /locus_tag="AC159B_05120"
     CDS             complement(6830..>7537)
                     /locus_tag="AC159B_05120"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR3377161.1"
                     /translation="VAASQTGVAPEVITFLPDVEVLVTRFISGRCPSTAEMTSPEMVA
                     RVVESMHRYHSGPAFEGRFSPFRALEQYLRIAFRYRAPLPPDVDRMHQRLPEIEAAVR
                     GGAVIGPCHNDLWGPNLIDDGTRIRIVDWEYAGMGDVYFDLANLAVHHDLSDAQDEGV
                     LRSYFGEVTDRGFARVKLLKIVAELREAMWYMVGVNVLPTDFDFLGSATTHFERYGRA
                     LDYRRVSAWLAQALSSA"
BASE COUNT         1374 a         2518 c         2318 g         1327 t
ORIGIN      
        1 gagatcggaa cgccctccgg acggttcgat cctccaacgc ctgagcagat ggacgccgct
       61 cttctcaaag gcacgggtgc atctccgggg aaagccacgg gcacagcccg cgtcatcgtc
      121 ggcccggagg agttcgtgcg cgtaagaccg ggcgacattc ttgtatgtcc gtcctcaaac
      181 ccttcatggg tcttgctctt cggaatcatc cgcggactcg tgaccaacac cggcggcgtg
      241 ctctcccacg ccgcggtagt cgcgcgggag tttggcgtgc cggcggtggt cggcacgggt
      301 gaggcaacgc ggcgtatctc cgatggccag agtattgagg tcgacgggac ggcgggcgag
      361 gtcaggatct tgtgaccgcg gccatcatcg cgatcgctgc cggcgcgatt ctcgtgccgc
      421 tgaactccac gatgctgtcg gtggccctcc ctgcgatcat ggccgacttc tccgtgaccg
      481 cgagcaccgt ctcgtggctc gtgacggcat acctggcaac agtggcggtc gccatgaccg
      541 tgagcggcaa cgccggagac cgtctcgggc acagacgcat gtttctggcc ggcgtcgcag
      601 ggttcgcggc gagctccctc ctcggcacgg tggcgtggtc atttccgatc cttgtggtgt
      661 cgcgagtgtt gcaggccgcc agcggtgcgt cgatcaccac gaattcgatc ggcctgatcc
      721 gagctctatc acctgaacac cggcggggcg cgagctttgg gatgttcgag atgcttatcc
      781 tgaccagcgc cgccgcgggg ccgtttgtgg gcggcctgct cacgagcgcc ttcggctggc
      841 ggtcgttgtt cgtcatcgcg gttccgatcg ccaccttctc cgctctgctc ttcaaagctg
      901 tcatcccgct gcaggaacgg cgcgaggcat tggccccggt cgatatcggc acactgttga
      961 ggcagtcgct gggtctgttc cgcgtgaggg cgttcgccgc ggctgttgcc gccgtcttcg
     1021 gatcgacggt gatccttcac acgtcgatga tcctcatccc aatcttcacg cagtcgctgc
     1081 tgcgcgcgtc ggccgccgtc agcggcatcg tcatgttcgt gtactttgga acgagcgccc
     1141 ttatctcacc aatgggcggc cgtctttcag ataacgcggg ccggcggttc ccggcggtcg
     1201 ccggcatgat ctgcaccgct gcgggactcg ccgcactgtc caggtgggca gggtcggtta
     1261 cgatcccgca ggtggcggtg tttctgtcgc tcatgggcct gggttcgggg ctgagcggca
     1321 cgccacgcca aacgtcggcg attgaaagtg ttccaccgag cgcgaccggc atggccgcgg
     1381 gaacgtattt caccatgcgc tatctgggcg gcgtggttgg cgcgaccctt gccggggtca
     1441 tgttgaggga gggtgtgacg acgcctgccg tggcccgagg cttcgggctc ctggctctcg
     1501 tcgcgtgcgc cgttgcggtt gtctctaccg gcctcaccgg gcggtctgca gttagccact
     1561 cgaggtcatg aaatgtcctt cgccggtgcc aggcaccggc gaagacaact gttcggtacg
     1621 ggaggagctt tcatgacgaa attcaccgcc ctggaagagt tcattctgga gaagatggcg
     1681 gagacgaagc tgcccggcgt cagcatcgcg ctggtcgatc aaggcgaggt cgcgtacagc
     1741 cgcggttttg gcttccgcga cgtcgagcgc tctctgccgg ccacaccacg cacgctgtac
     1801 ggcatcggca gcgtcacgaa atcgttcacc tgcatcgctc tgatgcaact gcaggagcag
     1861 ggcaaactcc gggtggacga cccggtagat cgctacgtcc cgcttaccgt gcgtcccatg
     1921 ggcgaccccg tgcgcattca tcacttcatg tcacacagca gcggaattcc cgcgctcgcc
     1981 tacgcagaag cggtcatccg gcacagtgtc ggcgcctcgg attcgtatgt ccccattggc
     2041 agctacgaag acatgctgaa cttcgtgaac ggcgcggaga aatggacgca cgcgcggcct
     2101 ggggagcgat ggttctatct gaacgagggc tacgtgctgc tcggcgcgat cattgagaaa
     2161 gtaagcggcc gaccctacgc tgattacatc cacgagcacg tcatcgcccc cctgcagatg
     2221 gaacgcacgt cgttcacgcg ggaagatctg cagcgagaaa cggatgctgc gaccccgtac
     2281 gtcgtcacga aggacaagga acagatcccc agccgctatc tcaacggaaa gctcagcagt
     2341 gacggcgggc tgatgagcag cgttgaggag atggcccgct acgtgatgat gttcctggac
     2401 ggcggccgcg ggccgcgcgg ggcgatcgtc agcggcgatt cattgaaggc catgatgtcg
     2461 ccacacgttc ccctgccgct cgaggtctat ccggcggcgc gtccggttcc gagcggacat
     2521 tacgggtacg ggctgagcat gaaccccgat ttctatggcc acacggtggt cggccacggc
     2581 ggcagcgtgc tcgtgagcac ggcgcacatg gcgttcattc ctgaccgacg gatcggcgca
     2641 atggtgctgg ccaacggcag cggctacccg ctgcagtaca ttgccttctc cgcgctcgcg
     2701 acgatgctgg gggaagaccc gtgggaatat cctgcgctgc gggtcgagcg caccctcggg
     2761 caactcaccg gttcgtatga gacttaccag cacacttacc cggcgacggt aacgcggaac
     2821 ggcgacttcc tgctgattac tatccgcaac agacacacgg agcagatcgt gccgctgatc
     2881 ccggaagatc tcgacccact gcgcccccgc tttttcaccc tcgccggcgg gcgacgattg
     2941 ccggtggaat tctcggtgaa caatggggac gtcgaactca tctacgagcg gtacaagttc
     3001 cgccgcacag gacggctcac atcttgaacg gccccgccgt caggttgtgg cgatatggaa
     3061 ctgggccttt gtgtacgtcg ccgtcctccc gcagcccgga cacgtgagtt cgtgcccgcg
     3121 atctgccggg atttcataga ccgcgcccgt gagctgaagg cctgcgggca cctgccttcc
     3181 acagttcttg caggatacgt agagcggttc tagattatcc gcagtcccca tcgtccaccg
     3241 ttcctgaatg ccctctgacg tcgtctcact ctgcgatgaa gatgttcttc agttcagtgt
     3301 acagttccag agcgtgcatg cccagttcgc gtccaaaccc gctcatcttc attccgccga
     3361 acggtgcttc ctggtgcacg ctgtggttac tgttgatgct gatgacccct gtctttatcg
     3421 cgcgggccat cctgagagcc cgaccgatgt cgcgcgtcca gatcgaagcg gacagcccgt
     3481 agggtgagtc gttcgccatc cgcgccgctt cggcttcgtc tgtgaatggg atggcgcaga
     3541 cgacgggtcc aaagatctct tcctgcgcca cgcgcatcgc gttcctgacg cccccaagca
     3601 ccgtcggcaa tagatacgcc ccacgcgcca gcgcagggtc ggcaggcgga cgtccgccgc
     3661 agagcaccgt cgccccctcc cgtgcgccga cgtcaacgta cccgcggacg cggtcacgat
     3721 gggtgagaga aatcagcgat ccgatctggg tctcttccgc cagcgggtcc ccggtgcgca
     3781 actgttcagt ctgttcgacc agccccgcca gcgtgcgctc gtaaatcgag tgctcaatga
     3841 agagccggct gcgcgcgcag cagtcctggc cggcattcgc aaagaccgcg gataccgcgc
     3901 ccggaatcgc acgttccgga tcggcgtcgg caaagatgac gttgggtgat ttcccgccca
     3961 attcaagaga cacgcgcgtc atcgtgtcgg cagccgcttt catgatctcg atgccggtcg
     4021 tggtttcgcc tgtgaatgcg atcttgccga gatgtggatg acggaccatt gccgcgccgg
     4081 tcgttccacc tggacccgtg atcacgttga ggactcccgc gggcagtccc gcctccagcg
     4141 caattgcgcc gagggccagc gcggtgagcg gtgtggcgct cgcgggcttg agcacggcgg
     4201 cattgccaac agccagggcg ggcgcgacct tccaggacgc gatcatcagc ggaaagttcc
     4261 acggcacgat caatcccacg accccaatcg gttcccgcag tgtgacgtcc aggcctcgat
     4321 ctgtcacagg gatggtctcg ccgaaccact tggtggccgc tccaccgtaa tattcgaagc
     4381 actccgccgc ggcgagcact tccgcccgag cgtcccgaat gggcttgccg gagttcagca
     4441 cctccaggcg cgccagggat tcaactcgct cccgcagtag cccggcgatg cgcaccaaca
     4501 cgcgtccgcg ctcgccggca gacatctgcg cccatttacc cgcttcgaaa gcgcgacggc
     4561 ctgcttgaac cgcgcggtct acatcttcct cgcccgcaat cgagacactg gccacaactt
     4621 cacccgtggc cggattagtc acggtggtcg tttgccccga aagggcgtcg atcccgtgtc
     4681 catcgatgat cagctgatat gcctgctgca tcacggtcat gtgcaaaagt cctccttact
     4741 ggttcgcctg cagcatccgg cgatagtcac tccggggtca cgtacgcggc ggtgatcccg
     4801 ccgtcaacaa ccagcgctgc acccgtcatc agcgaggact catcggaggc cagccacagg
     4861 gccgcccgcg cgatctcctc tgcctggccg aagcgcccga tcggaatatg cacgacccgc
     4921 cgggcacgcc gttgtgggtc cgcgagcagc tctgccagca tcggggtctc aactgggccg
     4981 gggcatagag cgtttgccct gatccccttg cgggcatatt caacggcgat ctcccgcgtg
     5041 attgacagca cgccaccctt gctggcggtg tatgcgatct ggggggtggc ggcccccatc
     5101 agcgccacga atgaggccac gttgatgatc gatcctcccc cagagcgcag cagcgccgga
     5161 atgccatatt tgcatcccag ccacacgccc ttgaggttga tgtcgatcac ccggtcccag
     5221 gtcgcctcgg acgtctcgac cgtcccgcca tcctccgagt gaaatatccc ggcgttgttg
     5281 tacaacacgt tcaggcgacc gaaccgctgt actgcaaagc gcaccatcgc ctcagcctcg
     5341 gccgccttgg agacgtccgc gcggacgaac accgcgtcgc cgcccgcaga cgtgatctcc
     5401 ttcgcggtgt cctcaccggc gcgctcggtg acgtcggcaa tcacgacctt tgcgccttca
     5461 gccgcaaaca gcgttgccgc gacgcggccc atcccgctgg ccgctccagt gatgagggcc
     5521 actttgtcct gcaaacgcct ggtcgtcccc attagttgac gagctcctcc cctaatctgc
     5581 cgagccgccg gtctgcggga gcgccttgag cgcagcagat cgccgccgca gcgggatcat
     5641 ggcgatgagt ccggcgaggg ggcccagggc gaggagtgcg aacgcccagc cccagtgcgc
     5701 tgccggccgc aggcccgcgg ccttcgtcat atccaacacg atgccgacgg caagcggcgc
     5761 caacgccgtc gccgtccacc ccgtgagcat ctgcagactg aacgcacccc ccagcgagtg
     5821 cggcggcacg acctccatga gtttggccga gtacgagggc gagtccgcca gcgaggacac
     5881 tccgtacagg agcgcaatac cgaccaccag gatcagcgga gccgcaaaca accatccaaa
     5941 cgtgagggag cagaacaggc tagccccgag catcaaggtg gcgatctccg cgcggcccag
     6001 ccgatcggaa agccgacctc cgacgacggc gccgatcgcg cctcccagcc cgatcgatcc
     6061 ggcggccagc atcccgcccc acgcgagcgc atcaccgcgg acgctcccgc gcgcaaggag
     6121 catagcgacc atgaacggtg tcaaccatcc ccacatcccg aacagctccc agttgtgacc
     6181 ggtatagccg atgatcacgc gccgggcggg gcggttgccg agaacatcac ccacgtaccg
     6241 cgatggaccc aacggcttaa gcatcgcggg gagaggaata ctcggaacct tccaggccat
     6301 cacgagggcc ccaagaagtt caacggcgcc caccccggcc atcgtccagc gccacccgaa
     6361 gcgcggcgcc agcagtccca cgtaatacaa cggcacggca taagcgagca cgatcaccgc
     6421 gatgtaaatc cccgtcgccg ttcctcggcg gtcgcggggg atcgtctcag caatctgctt
     6481 gagcgcgggg aggtacagcc cgccgaacac aatgcccacg agcaggcgga agaacaatcc
     6541 ggacgcaaaa tcccgcgcgc cgaaggcgaa gaggaggtct cccagaccga ccagcgcccc
     6601 cgaccacacc atgatcggtt tgctgcgcac ccgatccagc agaaaaccca cggcgacgta
     6661 ggccgccagc gtccccaact ggaaaacacc cagagtcagg ccggcctgac tgctgctgag
     6721 cctccattcg gatgacagga taggaatgag gatcgagaag gtcaggctgg tctggtgcgc
     6781 caggaactgg cccagacaga gtcccgtaaa accagacgac caacttccgt tacgcactgg
     6841 agagggcctg cgccagccag gccgagacgc gacgatagtc gagagcgcgg ccgtaccgct
     6901 caaaatgcgt cgtcgcagaa ccgagaaaat caaagtctgt gggcaacaca ttcaccccga
     6961 ccatgtacca cattgcttcg cggagctcgg cgacaatctt cagtagcttg acccgggcaa
     7021 acccgcggtc cgtgacctcc ccgaaatagc tgcgcagcac tccctcatcc tgagcgtcgg
     7081 aaaggtcatg atgtacggca agattcgcca gatcgaagta gacgtcgccc atcccggcat
     7141 actcccagtc cacgatgcgg atgcgcgtac cgtcatcgat caagttgggc ccccagaggt
     7201 cattgtggca cggaccgatc acggctccac cgcgcaccgc tgcctcgatc tcagggagcc
     7261 gctgatgcat cctgtcgaca tcgggcggca gcggcgcacg ataccgaaat gcgatgcgca
     7321 ggtattgctc gagagcgcgg aatggtgaga accttccttc gaaggcaggt ccgctatgat
     7381 accggtgcat ggactcgacg acccgtgcca ccatctcagg gctggtcatc tcggctgtgg
     7441 aggggcagcg tccggagatg aatcgcgtga ccagaacttc cacgtccggc aggaaagtga
     7501 tgacctcagg ggccacgccg gtctggctcg ccgcgac
//
LOCUS       DBNGSB010000178         9386 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3808482, whole genome shotgun sequence.
ACCESSION   DBNGSB010000178 DBNGSB010000000
VERSION     DBNGSB010000178.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 9386)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9386)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9386
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3808482"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="AC159B_05125"
     CDS             <1..377
                     /locus_tag="AC159B_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0002196 - Ser-tRNA(Ala) deacylase activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HIR3377162.1"
                     /translation="FGALVTGGAIYPDRARMDFALEDLSKDRVAAIETEANRVIGESR
                     RILVRFVSREEFERSDLTRLARNLLPLEIQEVRVIEIEGFDAQADGGTHVANTSEIGR
                     LVVTKTENKGKTNRRLEIALAN"
     gene            574..1008
                     /locus_tag="AC159B_05130"
     CDS             574..1008
                     /locus_tag="AC159B_05130"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF039181.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative metallopeptidase"
                     /protein_id="HIR3377163.1"
                     /translation="MQWLPAHDIHSRMRRIVRALGLGYISPARIRCVRVSGSRANALA
                     RIWGLPPIFQDALRLRPHYVIEFMVPAFDRLSRREQDRVLIHELLHIPRTFSGGIRPE
                     RSPSLSINNRTVERYYRQYLAAMSKSTQSSQSGRSSQSSKSS"
     gene            complement(1055..2326)
                     /locus_tag="AC159B_05135"
     CDS             complement(1055..2326)
                     /locus_tag="AC159B_05135"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3377164.1"
                     /translation="MSVTWRRLLYGLGSFGSSLLQQTVLLWIFYYYAPPPGQGLPTRV
                     PAALMGLAMAIGRLVDAIADPPVAYLSDRLRSTRGRRRPFIIAGTPLMALAFVLLWRP
                     PDAAASTANFIYVTVVLAAFFFLFTAVLNPYTALLPEITGPGASRTAIATWQASFSLA
                     GLATAYVTSGILSSRFGFPVMGLVLAIPGLTALLTAGLSIREKIVSENAIPFWRALRG
                     ILANRRFRIFVLGLTLLWFGLSMVNLSLAFDVTVLMGLPRSAVGIVLGAAVGIALIAF
                     PGIYALARWKGKRNTLMLAMGTAAVIVPFIGAIGLIPVPLSRATQGYALVALAGPPLA
                     ALFVLPNAILADIAEAHAAATGERSEAMFFAFQGLVFNGTTSLAAAALGGILQWLGYS
                     PENPLGLRVVPVIAAITVAAGAFVFSRYPRD"
     gene            2352..3230
                     /locus_tag="AC159B_05140"
     CDS             2352..3230
                     /locus_tag="AC159B_05140"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001319.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HIR3377165.1"
                     /translation="MLTPNAQTRVVGIIGDPVAHSLSPRMQNAAFDALGLNWRYLAFR
                     VRSDALSYALRGLAALGLAGANVTIPHKETTVSLVDELDAIARRVGAINTIRVADGRL
                     QGFNTDALGLLDALTKDGGAEIRGKRCLVIGAGGGGRAAAFALAGASAANLMVLNRTV
                     SRARGLAEIVSRDFPRCRVDAGDLAADTVERSMEGTDIVIHATTATMSAAMGGSGGRE
                     EWLATVVRSLRPGMVVHDMVYTPRWTDLLNTARNSGATAVSGLSMLLYQGAKSFELWT
                     GRTAPIDVMRQALEQS"
     gene            3337..4458
                     /gene="aroC"
                     /locus_tag="AC159B_05145"
     CDS             3337..4458
                     /gene="aroC"
                     /locus_tag="AC159B_05145"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIR3377166.1"
                     /translation="MLRFLTAGESHGKALTAIIEGLPAGLPVSDEDIAPQMERRQAGY
                     GRSVRMKLEADRVEIVSGLIGGETIGGPLALRIENRDVRENEPPLTRPRPGHADLVGA
                     MKYGFTDVRRALERASARETAARVAVGAVCRRLLSEFGIAIFSHVVEIGGIGVQNRPQ
                     QWEEIPEIAEASELRCVDREAEEQMRSAIEDAKKRGDTLGGVFEVVALGVPPGLGSYV
                     HWDRKLDARLAQAVMSIHAIKGVEIGPGFDIARSSGLKAHDEIMYERGQGFHRDTNRA
                     GGTEGGVTTGGPLVVRGAMKPLSTLMSPLQSVDIATKESIKGAVVRSDVCAVPAAGVV
                     GEAMVAYVLADAFLEKFGADTLQDIRAAYEAYGRRLQEM"
     gene            4472..6097
                     /gene="aroB"
                     /locus_tag="AC159B_05150"
     CDS             4472..6097
                     /gene="aroB"
                     /locus_tag="AC159B_05150"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HIR3377167.1"
                     /translation="MRNIVLIGFMGTGKSEVGKLLARRLGWTFIDTDRRVEARERATI
                     AQIFSRRGEHYFRESESRVIAEMAGRRDTVVATGGGAVLRPENMMHLRRHGWVVSLVA
                     SADVLQQRLSNGKNRPLLRGDVQGSVARLLDQRRPLYRDADLLIDVSQGSPAKIVEAI
                     VSFMGKRERRVVAVRLEGRSYPVYVGDGILPLLPVDLDALRAGRKVAVVSHRAVLHSA
                     GRQLVTALRGWGYEVTTIEVPAGEESKSLAVAARILTRLARERFDRHSTVLALGGGVV
                     GDLAGFVAATYMRGVRLVQAPTTLLAMVDSSIGGKTGVNHAGVKNLVGAFFQPGTVVA
                     DVHLLSSLPDRELRSGLAEIIKTAVIGDAVLFEYLEQHLSAILRRDTEPLIEVVARCA
                     AFKARVVEADEMELGGRRILNYGHTIGHAVEVATRGGRLTHGEAIAIGMALEGKLAQR
                     LGTTDSGTVERQNGLLARAGLPVALRGVNIRAFWRALALDKKRLNGVLRAPLLVGIGE
                     VRHEQEVPNALLQEVLSRASGARRLRAKSQPAR"
     gene            6048..6566
                     /gene="aroQ"
                     /locus_tag="AC159B_05155"
     CDS             6048..6566
                     /gene="aroQ"
                     /locus_tag="AC159B_05155"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015254861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HIR3377168.1"
                     /translation="MPRVLVVYGPNLNLLGEREPHVYGHETMQAINERLSALAQREGA
                     EVEFYQSNTEGAIIDRLQESRKKVDAIVLNAGALSHYSYALRDAIAAITIPVIEVHMT
                     NVVARGEFRTTLVLAPACKGVIYGFGANSFLLGLGAAIDLTKERERGASPIATGDGAR
                     GRAVKGRKSGRR"
     gene            6579..6962
                     /gene="aroH"
                     /locus_tag="AC159B_05160"
     CDS             6579..6962
                     /gene="aroH"
                     /locus_tag="AC159B_05160"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011172959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HIR3377169.1"
                     /translation="MRGIRGATTVESNTGEAILAATRELLAKMASDNNVEPDELAAVL
                     FTCTSDLTAAFPAEAARQLNWRSVPLMSATEMAVPEGLPMCIRVLMLWNSARSQEEVV
                     HIYLKRAETLRPDLQPRKRANAPTR"
     gene            6965..8263
                     /gene="aroA"
                     /locus_tag="AC159B_05165"
     CDS             6965..8263
                     /gene="aroA"
                     /locus_tag="AC159B_05165"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIR3377170.1"
                     /translation="MELTVSPVDRLQGRVRVPGDKSISHRAAMLGAIASGPTTIYGFL
                     HAGDCMSTLRCLRALGVTVDETQDRIVVHGKGPRLHAPAAPLDAGNSGTTIRLLAGIL
                     AGQSFVTELTGDASLRSRPMDRIMEPLGRMGAQVQALGDNRFPPLRIAGGTLRGITYT
                     LPVASAQVKSAVLLAGLYAEGDTTVIEPVATRDHTERMLESFGCRIRRNGSRITVTPS
                     TLSTRDVTVPGDISSAAFLLAAAAARRGSSIAADGVGINPTRTRFLDVLASMGAGTEI
                     VGRDERSGEPVGSVKVRGLELKGTRISGAVTSGVIDEIPVLAVIAAVAEGETAITDAA
                     ELRVKESDRIKTLAAGLRVLGADVTERPDGMVIRGGILQGGRVEAGGDHRIAMAFAVA
                     GLLARGPVTVSGAETIAISFPGFVEALQAVAGSPAVAASR"
     gene            8271..9224
                     /locus_tag="AC159B_05170"
     CDS             8271..9224
                     /locus_tag="AC159B_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017238806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3377171.1"
                     /translation="MSSPAAVASLAQRIRANIERVLVGKGDVIDFALTALLADGHLLI
                     EDVPGIGKTMLAKAMSRSLGCTFRRLQCTPDLLPADVTGIHYFNQKTQEFEFRPGPVF
                     TNILLADEINRATPRTQSALLECMEERQVTLETGTVPLGRPFMVMATQNPVELQGTFP
                     LPEAQLDRFLMQLPIGYPGADDEAEILRRFRAMNPLDHLEAVTSADEVAQMQRAVREI
                     HVSPAVEGYIVEITRATRAHPEIELGASPRASLGLHRTAQALAAIRGRAFVLPDDVKV
                     LVPYILPHRLITTSQARLRDRGAHEVAQEILKSVPAPVEAE"
BASE COUNT         1798 a         2802 c         3095 g         1691 t
ORIGIN      
        1 tgtttggcgc gctcgtgacc ggtggggcaa tctatccgga tcgggcgagg atggattttg
       61 cgctcgagga cctcagcaaa gatcgcgtgg cggccatcga gaccgaagcg aaccgagtaa
      121 tcggcgaaag ccgccgcatt ctggtgcggt ttgtttcgcg tgaagagttc gagcggtcgg
      181 atctcacccg cctggccaga aacctgctgc cgctggagat ccaggaggtc cgggtcatcg
      241 agatcgaggg gtttgacgct caggccgacg gcggaacaca tgttgcaaac acgagcgaga
      301 tcggcaggct ggtcgtcacg aagacagaga acaaagggaa gacgaaccgc aggctcgaga
      361 tcgcactggc aaattagcag ggtggcggcg caccgggaga tgtcggagtc tctccccgac
      421 tgcaactaat acgggacccc tcagactccg ccatccccag tcgcgccgtc atgattatgc
      481 gatcgagcca gtggattttg agtctgcggt gtaggattgg caggactcgc tctttcgaga
      541 agccaaccca gtaagtacat cgcggcgatc tccatgcagt ggcttcctgc ccacgacatt
      601 cactcccgga tgcggcgcat tgtccgagcc ctagggttgg gctacatcag cccggcccgg
      661 atccggtgcg tgcgtgtttc cggttcccgc gcgaatgcgc tggcgagaat ctgggggctg
      721 ccgccgatct ttcaggatgc acttcggttg cggccgcact atgtcatcga gttcatggtt
      781 cccgcgttcg accgtctgtc tcgacgcgag caggaccgcg tgctcattca cgagttgctg
      841 cacattccgc gcacgttcag cggtgggatc cgtcctgagc ggtcaccttc gttgagtatc
      901 aataacagga ccgttgagcg atattatcgg cagtacctcg ccgccatgag caaatctaca
      961 caatcttctc aatctggacg atcttcacaa tcttccaaat catcatgatc taggttgtgc
     1021 agatcgtgga gattgtgtag atcgtgcaga ttgtctagtc ccggggatac cggctgaata
     1081 cgaacgcccc ggcagccact gtgattgcgg cgatcacggg caccacccgt agtcccagcg
     1141 gattctccgg ggaatagccg agccactgca agattccgcc cagggccgct gcggcaaggc
     1201 tcgttgttcc attgaacacc agcccctgaa aggcaaagaa catggcttca ctccgttcac
     1261 ctgtggcggc tgcatgcgct tcagcgatgt cggccaggat tgcgttcggc agtacaaaca
     1321 gcgctgccag cggtggacct gcgagggcga ccagcgcata tccctgcgtt gcgcgtgaaa
     1381 ggggtacggg gatcagcccg atcgctccga tgaatggcac gatcaccgcg gcagtcccca
     1441 tagccagcat caaagtgttg cgctttccct tccacctcgc tagggcgtag attcccggga
     1501 atgcaatgag cgcgataccc acggccgcgc cgagcacgat tcccaccgca gacctcggca
     1561 gccccatcag cacggtgaca tcgaatgcca gcgagaggtt caccatgctg aggccgaacc
     1621 acaagagcgt cagacctaag acgaagatcc tgaaacgtcg gtttgcgaga atacctcgaa
     1681 gtgcacgcca gaacgggata gcattttcag aaacgatttt ctcgcgaata ctgagcccgg
     1741 cagtcaagag tgcggtcagt ccggggatgg cgagaaccag gcccatgacc gggaagccga
     1801 accgtgagga gagtattccg gatgtcacgt acgcggtcgc gagtccggcc aggctgaatg
     1861 atgcctgcca cgtggcgatc gccgttcgcg aagcgcctgg accggtgatt tccggcagca
     1921 gcgcggtgta tgggttcaac acggccgtga acagaaagaa gaacgccgcc agcaccacag
     1981 tcacatagat gaagtttgcg gtcgatgctg cggcgtccgg cggtcgccac aacaggacga
     2041 acgccagcgc catcagcggc gtgccggcga tgataaacgg gcgccgccgt ccccgcgtgg
     2101 accggagccg gtcgctgaga tatgccaccg gaggatccgc gatggcatcg accagccggc
     2161 cgattgccat agccagaccc atgagtgcgg ccgggacgcg cgtcggtagg ccctgccccg
     2221 gcggcggcgc gtaatagtag aagatccaaa gcagcacggt ctgctggaga agactgctgc
     2281 cgaaactccc caggccgtac agcaatctcc gccaagtaac cgacatcact catttagtat
     2341 aattgcgtgc aatgcttacg ccgaacgcgc agacgcgagt ggtgggcatc atcggagatc
     2401 ctgtcgccca ttcactttct ccccgcatgc agaatgctgc gtttgatgcc ctcggactca
     2461 actggcgcta cctggcattc cgcgtccgat ccgacgcgct ttcgtacgca ctgcggggtc
     2521 tcgcggcgct cggcctggcc ggtgcgaacg tcaccatccc ccacaaggag acgaccgtct
     2581 cccttgtcga cgaactggac gccatcgccc ggagggttgg tgcaatcaat accatccgtg
     2641 tggccgatgg acgtctgcag gggttcaaca ccgacgcgtt gggcctgctt gacgctttga
     2701 ccaaggacgg gggcgccgag atcaggggca agcggtgcct ggtgatcgga gcgggaggag
     2761 gcggacgggc cgcagcgttt gccctggccg gcgcatcggc ggcgaacctg atggttctca
     2821 accgcacagt ttcccgggcg cgtggtttgg cagagatcgt gtcgcgagac tttccacggt
     2881 gccgggttga tgcgggtgat cttgccgcgg ataccgtcga gaggagcatg gagggcaccg
     2941 acatcgtgat tcatgcgacg acggccacga tgtctgcggc gatgggcggg agcggcggtc
     3001 gcgaggaatg gctggcgacg gtggtgcgta gtttgcgccc gggcatggtc gtgcatgata
     3061 tggtgtacac tccgagatgg actgacctgc tcaacacggc acggaactcc ggcgcaaccg
     3121 ccgtgagcgg gctttcgatg ctgctctacc agggggcaaa gagctttgag ttgtggaccg
     3181 gccggaccgc tcccatagac gtaatgcggc aggccttgga gcaaagctga agagctggag
     3241 agctaaagag ctgaacagca gaagaaacga tgcttggggt tgctttccag cttttcagct
     3301 ctccagcttt ccagctgtag tggagggatc gaccggatgt tgaggttcct gacggcaggc
     3361 gaatctcatg ggaaggctct cactgccatc atcgaaggcc tgccggctgg tctgcccgtc
     3421 agtgatgaag acatcgcccc gcagatggag cggcgccagg caggttacgg ccggagcgtt
     3481 cggatgaagc tggaagcaga ccgggtggaa attgtgtccg gcctcatcgg cggcgaaacg
     3541 atcgggggcc ctctggctct gcggatcgag aatcgcgacg ttcgcgagaa cgagcctcca
     3601 ctcacccgcc cccgacccgg gcacgccgat ctcgtcgggg ccatgaagta cggcttcacc
     3661 gatgtgcggc gcgcattgga acgtgcgagc gcgcgggaga ctgccgcccg ggttgcggtc
     3721 ggggctgtgt gtcggcgttt actcagtgag ttcgggatag caatcttcag tcacgtcgtt
     3781 gagatcggcg gcatcggtgt gcaaaaccgg ccccaacagt gggaagagat ccccgagatc
     3841 gctgaggctt ctgaactgcg ctgcgtggac cgcgaagccg aggagcaaat gaggtctgcc
     3901 atcgaggatg cgaagaaacg cggggacacc ttgggcgggg tcttcgaagt cgtggcgctt
     3961 ggcgtcccgc cggggctagg gagctacgtg cattgggacc gaaaactcga cgccaggctc
     4021 gcgcaggccg tgatgtcgat ccacgccatc aagggcgtgg aaatcgggcc ggggttcgac
     4081 atcgcgcgtt cttccggact gaaggcccac gacgaaatca tgtacgaacg aggccagggc
     4141 ttccaccgcg acacgaatcg cgccggggga acggaaggcg gcgtgaccac cggcggtccc
     4201 ctggtcgtcc gcggcgcaat gaagccgctc tccaccctca tgtcaccgct tcaatccgtg
     4261 gacatcgcca ccaaagagtc gatcaagggt gccgtcgtga ggagcgacgt atgcgcagtt
     4321 ccggcggccg gcgtggtcgg cgaagcgatg gtggcctacg tgctcgccga tgcctttctg
     4381 gaaaagttcg gtgcagatac gctccaggac atacgcgccg catacgaagc gtacgggcgc
     4441 cggctccaag agatgtaatc gaatcacgtt gatgcgtaac atcgttctca tcgggtttat
     4501 gggcacaggg aagagcgagg tcggcaaact cctcgcaaga cgcctgggat ggacgtttat
     4561 cgatacggac cgccgggtgg aggcacgaga acgggcgacg attgcgcaga ttttctctcg
     4621 ccgcggtgag cactattttc gcgagagcga atcgcgggtc atcgccgaga tggcgggacg
     4681 acgggatacc gtcgtggcga ccggcggcgg tgccgtattg cgtccggaga acatgatgca
     4741 cctccggcgc cacggctggg tggtctcgct ggttgcttcc gcggacgtgc tgcagcagcg
     4801 tttgagcaat gggaagaatc gtcccctctt gcgcggggat gtgcagggca gtgtggcaag
     4861 actgctggat caacgccggc cgctgtaccg cgatgctgat ctcctgatcg acgtatcaca
     4921 aggatctccc gcgaagatcg tggaggccat cgtctcgttc atgggcaagc gcgaacgacg
     4981 agtggtagca gtccggttgg agggccgctc ctatcccgtt tacgtcggtg acgggatcct
     5041 gccgctgctg cccgtcgatc ttgacgcgct cagagcgggc aggaaagtgg cggtcgtgtc
     5101 ccaccgcgcc gtcctgcact ctgccggtcg gcaacttgtg acggcactga gaggctgggg
     5161 ctacgaggtc acgacgatcg aagtccctgc gggagaagag tccaagagtc tggccgtcgc
     5221 cgcgcgcatt ctcacgcgcc tggcgcgcga acggttcgac aggcacagca cggtcctggc
     5281 tctcggcggt ggggtggtcg gggatcttgc cggattcgtc gcggcgacat acatgagagg
     5341 tgtacggctt gttcaagcgc ccacgacgtt gctggcgatg gtggacagca gcattggcgg
     5401 caagaccggg gtgaatcacg ccggcgtcaa gaatctcgtt ggcgcatttt tccagcccgg
     5461 caccgtggtc gcggatgtcc accttctctc atcgcttccg gaccgcgaac ttcggagcgg
     5521 gctggccgag atcatcaaga ccgcggtgat cggagatgcg gtccttttcg agtatcttga
     5581 gcagcatctg tccgccatcc tcaggcgcga caccgaaccc ctgatcgagg tggttgcgcg
     5641 ctgcgctgca ttcaaggccc gcgtcgtcga agcggatgaa atggaacttg ggggacggcg
     5701 catcctgaac tacggacaca ccatcggcca cgccgtggaa gtggcgaccc gaggtggacg
     5761 attgacgcac ggggaggcaa ttgccatcgg catggcgctg gaggggaagc tcgcgcagcg
     5821 gctgggcacg acggattctg gaacggttga gcgtcagaac ggtctcctgg cgcgcgccgg
     5881 actgcctgtg gccctgcgcg gcgtgaacat ccgcgcgttc tggcgcgcgt tggcgctgga
     5941 caagaagcga ttgaacggcg tgctgcgcgc accgttgctg gtaggcatcg gcgaggtacg
     6001 gcacgaacag gaagtgccaa acgcgcttct ccaggaggtg ttgagccgtg cctcgggtgc
     6061 tcgtcgtcta cgggccaaat ctcaacctgc tcggtgagcg ggagccccac gtttacggac
     6121 acgagaccat gcaggcgatc aacgagcgcc tcagcgccct ggcgcagcgt gagggtgctg
     6181 aggtcgagtt ctaccagtcg aatactgaag gcgcgatcat cgaccggctg caggaatcgc
     6241 ggaagaaggt cgatgcgatc gtgctgaacg ccggcgccct gtcccattac agttatgcac
     6301 tccgcgatgc catcgcggcg atcacgattc ccgtcatcga ggttcacatg accaacgtgg
     6361 tcgcgcgcgg agagttcagg acgactcttg tcttggcccc ggcctgcaaa ggcgtcattt
     6421 acgggtttgg cgcgaatagc tttctccttg gcctgggggc ggcgatcgat ctcacgaaag
     6481 aacgggaacg cggtgcgtca ccgattgcca ctggggacgg tgcgcgtggc cgtgcagtca
     6541 aagggcgcaa gtccggccgg cggtgatggt tggtgcacat ccgcggcatc cgcggcgcga
     6601 ccaccgtcga gagcaacacg ggtgaggcga tcctggccgc gacgcgcgaa ctgcttgcca
     6661 aaatggcgtc ggacaacaac gtcgagccgg atgaactcgc agcggttctc ttcacgtgca
     6721 cctctgatct caccgcagcc ttccccgcag aagccgcgcg gcaactgaac tggcggtcgg
     6781 tccccctgat gtcggccacg gagatggcgg taccggaggg gttgccgatg tgtatccgcg
     6841 tcctgatgtt gtggaatagc gcgcggtcgc aggaagaagt cgtgcacatc tatttgaagc
     6901 gtgctgagac gctgcgaccc gacctgcaac cccgcaaacg cgcaaacgcg ccaacgcgat
     6961 aaccatggaa ctcacggtga gtcccgtcga tcggcttcag gggcgagtcc gtgtgcccgg
     7021 tgacaaatct atctcgcacc gcgccgcgat gctgggcgca atcgcgagcg ggccaaccac
     7081 catctatgga tttcttcacg ccggcgattg tatgagtacc cttcggtgtc tgcgggcgct
     7141 gggggtaacc gtagatgaaa ctcaggaccg gatcgtggtg cacggcaaag gcccccgatt
     7201 gcacgcgccc gccgcaccgc tcgacgcggg caactcggga acgaccatac ggctgctggc
     7261 cgggatcttg gcgggacagt catttgtcac ggaactcacg ggtgatgctt cgctgcgatc
     7321 acggccgatg gacaggatca tggaacccct cgggaggatg ggcgctcagg tccaagccct
     7381 cggcgataat cgctttccac cgttgcgcat cgcaggcggc acgctgcgag ggatcacgta
     7441 cacgctgccg gtcgcaagcg cgcaggtaaa atcggctgtg ctgctggccg gactctacgc
     7501 agaaggcgac acgacggtca tcgagcctgt agctacgcgc gaccataccg agcggatgct
     7561 cgaaagtttc gggtgccgca tccggcgcaa cggaagcagg atcaccgtaa ccccatcgac
     7621 gctgtcgacc agagacgtca cggtgcccgg cgatatttcg tcggccgcgt tcttgcttgc
     7681 cgctgcggcg gcgcgccggg gctcatcgat cgctgcggac ggcgtcggga tcaatcccac
     7741 gcggaccaga ttcctggatg tgctggcatc catgggcgcc ggcaccgaga tcgttggacg
     7801 ggacgagcgt tccggtgagc ccgtcggctc ggtgaaggtg cgaggcctcg agctgaaggg
     7861 cacgcggatc tccggggccg tgacgtccgg cgtcatcgac gaaattccag ttcttgcggt
     7921 gatcgcggct gtcgcggaag gggagacggc gatcaccgac gctgcggagc ttcgcgtcaa
     7981 ggagtccgac cgcatcaaga cgctcgcggc gggccttcgc gttcttggag cggatgttac
     8041 cgagcggccc gatggaatgg tgattcgcgg cgggattctc cagggcggcc gcgtcgaggc
     8101 aggcggcgat caccggatcg cgatggcatt tgcggtcgcc ggcctcctgg ctcggggtcc
     8161 ggtgacggtc tccggcgcag aaaccatcgc catctcattc ccgggattcg ttgaagcgct
     8221 gcaggcggtt gcgggaagcc cggccgtcgc ggcatctagg taaggatcgc atgagctcac
     8281 cggcggcagt cgccagtctg gcacagcgga tccgcgccaa catcgagcgc gtgctcgtgg
     8341 gcaagggcga cgtgattgat ttcgcgctca ctgcgctcct cgccgatggg cacctcctca
     8401 tcgaggacgt gccgggcatc ggcaagacga tgctggccaa agccatgtcc aggtcactcg
     8461 gctgcacgtt ccgccggctg caatgcacac cggacctgct gcccgccgac gtgaccggaa
     8521 ttcattactt caatcagaag acacaagagt ttgagttccg tcccggccca gttttcacga
     8581 atattctcct ggcggacgag atcaaccgcg ccacgccgcg tacacaatca gccctgctgg
     8641 agtgcatgga ggagcgccag gtgacgcttg aaacaggcac ggtgcctctg ggccgtccct
     8701 tcatggtcat ggcgacccaa aaccccgtcg agttacaggg gacgtttccg ctgcctgaag
     8761 cgcaactcga caggttcctc atgcagctgc ccattggtta tccgggcgcg gacgacgaag
     8821 cagaaattct caggcggttc cgcgccatga atccgctgga tcatttagag gcggtgacgt
     8881 cggcagacga agtggcgcag atgcagagag ccgtgagaga aatccacgtc agccctgcgg
     8941 ttgaagggta cattgttgag atcacccggg cgacacgtgc tcacccggag atcgagctcg
     9001 gcgccagccc gcgcgccagt ctggggcttc atcgaacggc gcaggcgctg gccgcgattc
     9061 gtggccgggc attcgtcttg cccgacgacg tcaaagtcct cgtcccttat atccttccgc
     9121 atcgtctcat caccacatca caggcaaggc tgcgagatcg cggcgcgcac gaggtcgcac
     9181 aggaaattct gaagtccgtt cctgcccccg ttgaagcgga gtaagtctca gaaacccgag
     9241 cccacacggc cggccgtatc accggtggcg ctgctggagc gcgagcgccg gcagttggag
     9301 cgctaccttt ttaagtcagg cacgagcgcc ttcaaactct cgcgcgcgca gtttacgcag
     9361 accggcgtga tgaacgactg gtcggt
//
LOCUS       DBNGSB010000179         4186 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_741746, whole genome shotgun sequence.
ACCESSION   DBNGSB010000179 DBNGSB010000000
VERSION     DBNGSB010000179.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_741746"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="AC159B_05175"
     CDS             complement(<1..1128)
                     /locus_tag="AC159B_05175"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl-D-amino-acid deacylase family protein"
                     /protein_id="HIR3377172.1"
                     /translation="MARLHDVIIRNARIVDGTGSLPAAGDIAMDGDQIARVGSVLGGS
                     GRVELDADGLVVAPGFINMLSWANVSLLADGRSMGDIKQGVTLEVMGEGSSMGPLNDR
                     MKRELVEHQGDIKYDVAWTTLREYLDHLVRRGVSTNVASFIGSATPRVHELGYEDRLP
                     SAAELDRMRRLVAQAMQEGAVGISSALIYPPASFSDTNELIALAEVAAEYGGMYISHI
                     RNEEATVLAALEEFFEVVRSTGITGEIYHLKVSGKANWAKFDPMIARIEHARSSELPV
                     TADMYPYIASSTGLDTGIPDWAHAGGQEALETRLRDPAARGRIKEEFALFTTPENIIV
                     VSVKQDVFKPLIGRTLAEIAADRGQDYRDTVMDLIAQDHSRI"
     gene            1202..2596
                     /gene="glmU"
                     /locus_tag="AC159B_05180"
     CDS             1202..2596
                     /gene="glmU"
                     /locus_tag="AC159B_05180"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015740033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HIR3377173.1"
                     /translation="MGIEFLGWGGYMERVHAVVLAAGKGKRMKSDLPKVLHRVSGQAL
                     VTFVLDTLRAAGVEDPVIVVGHGAEGVRAIGGPRARYVDQVEQRGTGHAVMQALPLLT
                     ERSDPVLVLYGDTPLLTPHTIRALIEQHRRTAAAVTMLTAKLSDPAGYGRVIRDGGGR
                     VQRIVEDADASAEEKQVREINAGTYVFEPAALREALKGLAPHNAQGEYYLTDTVSWLL
                     SQGRAVSALVGSPEETMGVNSRRELAQVEAVMRRRILERLMDEGGTISDPLTTYVHAG
                     VVVGRDSVIHPQSHLEGSTRIGSGCVIGPQARLVDSTLLDEVTVVASTVEGSTIGEGT
                     RVGPYSHLRPGTQVGRFVEIGNFAEMKNTTVGDYTKVHHKSYLGDATIGERVNIGAGT
                     VTCNYDGKGKYPTVIEDGAFIGSDTMLIAPVRIGKGAVTGAGSVVNKDVPPGALAVGV
                     PARVIKYVTANTER"
     gene            2571..3569
                     /locus_tag="AC159B_05185"
     CDS             2571..3569
                     /locus_tag="AC159B_05185"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HIR3377174.1"
                     /translation="MSQRTLSVEDFERPRVSAPQPRLKLFSGSGNPVLAQQIAAALDV
                     PLADMSIFRYADGEIGVRIEESVRGEDVFVIQPTCTPVNENLMELLVITDALRRASAD
                     RITAVLPYFGYARQDRKMKPREPISAKLVANLITAAGCNRILTIDLHAGQIWGFFDIP
                     LDHLPARMILAEHFRGRALENLVIVSPDVGGVQRAREFAAQLGAPIAIIDKRRDRPNQ
                     VKEVVHVIGKVYRKTAIIVDDIVDTAGTLTMGAQALRKRGVGEVYACATHAILSGPAT
                     ARLQDSPILDLVVTNTIPIASEKLSEKITVLSVAPLLADAIRRIHENTSVSSLFSE"
     gene            3588..>4186
                     /locus_tag="AC159B_05190"
     CDS             3588..>4186
                     /locus_tag="AC159B_05190"
                     /inference="COORDINATES: protein motif:HMM:NF013146.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3377175.1"
                     /translation="MNIPPYLVAATVAFIVTYAISLEMQWIARRLGAVTPPGGRHIHT
                     TPIPRMGGLAIFGGVMLALLMALPIDSPVALVREPRYVILAVPYKPLVLPLLGVVVGA
                     VAITALGAVDDLRALPGRLKFPLIYAAAAIPVMFGLTTSFLTNPLQGTSVPLGVAGQI
                     FTVIWLGSMAIALNSIDGVDGLAAGIAGITGATLLMAAAP"
BASE COUNT          738 a         1332 c         1356 g          760 t
ORIGIN      
        1 gatccggctg tggtcctggg cgatgaggtc catgaccgtg tccctgtaat cctggccgcg
       61 atcggcggcg atttcggcga gcgtccggcc gatcagcggc ttaaagacat cttgctttac
      121 actcaccacg atgatgttct ccggcgtcgt aaacagggcg aactcttcct taatgcgtcc
      181 tcgcgccgcc gggtctctga gccgcgtttc cagcgcctcc tgtccgcccg cgtgcgccca
      241 atccgggatg ccggtgtcga gcccagtgga ggacgcgatg tagggataca tgtcggcggt
      301 cacgggaagc tccgaagaac gcgcgtgttc gatgcgtgca atcatcgggt caaacttggc
      361 ccagttcgcc ttcccggaca ccttgagatg gtagatttcg cccgttatcc ctgtcgagcg
      421 aacgacctcg aagaactcct cgagcgcggc gagcaccgtg gcctcttcgt tgcggatgtg
      481 cgagatatac ataccgccgt actcggcggc gacttcagcc agcgcgatga gctcgtttgt
      541 gtcggagaat gacgccggcg ggtagatcag cgcggaggag atgccgactg caccttcctg
      601 catcgcttgc gccaccagac gccgcatgcg atccagctcc gccgcggacg gaagacggtc
      661 ctcgtacccg agctcatgaa cacgcggggt cgcggacccg atgaaggacg cgacgtttgt
      721 agagacgccg cgccggacga gatggtccaa gtactcccgg agtgtcgtcc acgcgacgtc
      781 gtatttgatg tcgccctggt gctccaccaa ctcgcgcttc atgcggtcgt tgagcggtcc
      841 catggacgac ccctcgccca tgacctcgag cgtgacgccc tgcttgatat cacccatcga
      901 ccgtccgtcc gcaagcaggg atacgttcgc ccaactcagc atgttgatga aacccggtgc
      961 gacgaccagt ccatctgcat cgagttcgac acgaccgctg cctcccagaa ccgatccaac
     1021 ccgtgcgatc tggtcgccgt ccatcgcgat atctccagcc gccggcaggc ttccagtgcc
     1081 gtcgacgatc ctcgcgttcc ggatgatgac gtcgtgcagg cgtgccatgc ccactgcatt
     1141 ccccagcacg ctgcgtgcgc cctgtgaggg gtgccgtgtg tgaggtgcgg gaatgtaata
     1201 gatggggatt gaattcctcg ggtggggagg gtacatggag cgcgtgcacg ccgtggtgct
     1261 ggcggcgggg aagggtaagc ggatgaagtc cgacctcccc aaggttctgc atcgcgtcag
     1321 cggccaggcc ctcgtcacgt tcgtcctcga cactctgaga gctgcgggcg tcgaggatcc
     1381 cgtcatcgtt gtgggtcatg gcgccgaggg cgtacgcgcg atcggcgggc ctcgcgcacg
     1441 ctacgtcgac caggtcgaac agcgcggcac cggccatgcg gtgatgcagg cgttgcccct
     1501 gctgaccgag cggagcgatc cggtgctcgt gctctatggc gatacgccgc tgctcacacc
     1561 ccacaccatc cgcgctctca tcgagcaaca ccgccgcacc gccgccgcgg tgacgatgct
     1621 gacggcgaaa ctctcggatc ctgccggcta tggccgcgtc atccgcgacg ggggcggccg
     1681 cgtccaacgg atcgtcgagg acgcggacgc ttcagccgag gagaagcaag tgagagagat
     1741 caatgcgggc acttacgtat ttgaaccggc ggcgcttcgg gaggcgctca aaggtcttgc
     1801 cccgcacaac gcgcagggcg aatattacct tacggacacc gtaagctggc tgctgtcaca
     1861 ggggcgggcc gtcagcgcgc tcgttggttc tccggaagaa actatgggcg tcaactcgcg
     1921 cagagagctc gcacaggtcg aggccgtgat gcgccggcgg atcctggagc gcctgatgga
     1981 cgagggcggg acgatcagtg atccgctgac cacatacgtg catgccggcg tggtggtagg
     2041 ccgggactcg gtgattcacc cgcagtcgca ccttgagggg agtacccgga ttgggagcgg
     2101 ctgcgtaatc ggcccccagg caagactggt cgactcaacg ctcctggacg aggtcacggt
     2161 cgtcgcgtcc acagtcgagg gaagtacgat cggagagggg actcgcgttg gtccctacag
     2221 ccacctgcgt cccggcacgc aggtgggccg gttcgtcgag atcggaaatt ttgccgaaat
     2281 gaaaaacacc accgtgggcg actacacgaa ggtccatcat aagagctacc tgggcgacgc
     2341 caccatcggg gagcgtgtaa atatcggcgc gggcaccgtc acctgcaatt acgatgggaa
     2401 agggaagtat cccacggtaa tcgaagatgg cgccttcatc gggagcgaca ccatgctgat
     2461 cgcgcctgtg cgcatcggca aaggggcggt caccggcgcc ggttctgtcg tcaacaaaga
     2521 tgtgccgccg ggcgccctgg cagtcggggt cccggcccgc gtgatcaaat atgtcacagc
     2581 gaacactgag cgttgaggat ttcgaacggc cgcgcgtaag tgccccgcag ccgcgactga
     2641 aactgttcag cggcagcggc aaccctgtcc tggcacagca gatcgcggcg gcgctggacg
     2701 tcccgctcgc cgacatgagc atcttccggt acgccgatgg cgagatcggc gtgcgcatcg
     2761 aggaaagcgt ccgcggcgag gacgtgttcg tcattcagcc cacctgcacg cccgtaaacg
     2821 aaaacctgat ggagctcctc gtcatcaccg acgcgctgcg ccgggcctcg gcggaccgca
     2881 tcaccgcggt gctgccgtat tttgggtacg cccgccagga ccggaagatg aagccccgcg
     2941 aaccgatctc ggccaagctt gtcgccaacc tgatcactgc ggccgggtgc aaccgcatcc
     3001 tcacgatcga cctccacgcg gggcagatct ggggtttctt tgacattccc ctggatcatc
     3061 ttccggcgcg aatgattttg gccgagcact tccgggggcg tgcgcttgag aacctcgtca
     3121 tcgtgtcgcc ggacgtaggc ggggtgcagc gtgcgcggga gttcgcggcg cagctgggcg
     3181 cgccgatcgc catcatcgac aagcggcgtg atcgccccaa ccaggtcaaa gaggtcgtgc
     3241 acgtgatcgg aaaggtttac aggaagaccg cgatcatcgt ggacgacatc gtggatactg
     3301 cggggacgct gacgatgggc gcgcaggcgc tgcgcaagcg gggcgtgggc gaggtctatg
     3361 cgtgtgcgac gcacgcgatc ctatcgggcc ccgccacggc gcgcctccag gacagcccga
     3421 tcctcgacct cgtggtcacg aataccatcc ccattgcgtc cgaaaaactc agcgaaaaga
     3481 tcacggtgct gtccgtcgcc ccactacttg cggatgcgat caggcgcatc cacgagaata
     3541 cgtctgtgag ctccctgttt agtgaataag gcctgtagga acgctgcatg aacatcccgc
     3601 cctacctcgt cgccgccacg gtcgctttta tcgtcacgta cgcgatctcg ctcgagatgc
     3661 agtggatcgc ccggcgactc ggcgcggtga cgcccccggg aggccgccac attcacacga
     3721 cgccgatccc acggatggga gggctcgcga ttttcggcgg ggtgatgctc gccctgctca
     3781 tggcgcttcc tatcgattcg ccggttgcgc tcgtgcgtga accgcgttac gtgatcctcg
     3841 cagtcccgta caagcccctg gtcttgcccc tgctcggcgt ggtcgtcggc gcggtggcga
     3901 tcacagcgct cggcgcggtc gatgaccttc gcgcgcttcc cgggcggctc aagtttccgt
     3961 tgatttatgc ggcggcggcc attccggtga tgttcgggct gaccacgtcg ttcctcacca
     4021 atcccctcca ggggacatct gttccacttg gggtggcggg gcagatcttc acggtgatct
     4081 ggctcgggtc gatggccatc gctctgaact caatcgacgg ggtggatggt ctggccgcgg
     4141 gcatcgcggg gatcaccggc gcgacgctgc tgatggcagc ggcccc
//
LOCUS       DBNGSB010000180         4640 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_1661370, whole genome shotgun sequence.
ACCESSION   DBNGSB010000180 DBNGSB010000000
VERSION     DBNGSB010000180.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_1661370"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="AC159B_05195"
     CDS             <1..272
                     /locus_tag="AC159B_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3377176.1"
                     /translation="PEARRVVIAIRDSGVGVKESELERIFDRFVSSKPQGLGLGLAIS
                     RSIVEAHGGHLRAVRNDDHGLTFTIELPADGAETVPADASTRWAG"
     gene            complement(202..741)
                     /locus_tag="AC159B_05200"
     CDS             complement(202..741)
                     /locus_tag="AC159B_05200"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014973.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HIR3377177.1"
                     /translation="MEKRPAALEIVLRRKADMPKDLVAEFTEHADDAWITANGAHMVA
                     RAWTDLEYRARMLVDGMAAARELGFEFPEHHRNLVVLENTATVHNVIVCTLCSCTAYT
                     LIGAAPDSFLDAWTPPFGFPERPFSKRLLVAAIACSVAVLHAERRRTTAWAVPRVSRP
                     IAWRRRPGRSPRHLQAARW"
     gene            complement(768..1442)
                     /locus_tag="AC159B_05205"
     CDS             complement(768..1442)
                     /locus_tag="AC159B_05205"
                     /inference="COORDINATES: protein motif:HMM:NF014069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR3377178.1"
                     /translation="MSGSERPSFSGALAVGRKLDLPLPDSPAVRDAYAHAEAESQPWL
                     FNHVMRSWLYSAKLAQSRGLAPDAELVAVAVLLHDLGLARGGAPDRRFEVVGADIGRA
                     FALSHDMGERRAEVVWDSIALHTTPSIAQHKGADVACCQNGIACDYGGLGYQELSDDI
                     KKIILSAYPRLDMKNMLTTCLCGIAKNHPSTTRDNFIADFGIKYIPGYTRVSSVDLLH
                     QAPFAE"
     gene            complement(1475..2173)
                     /locus_tag="AC159B_05210"
     CDS             complement(1475..2173)
                     /locus_tag="AC159B_05210"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007789035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HIR3377179.1"
                     /translation="MSQAISVVVPVYEGVTQLDFTGPHQFLSRTPDLHVRVASLGGEP
                     VTADRLTFSNLDDLAAIESCDVLLVPGGGGCLAAIENDLFVKEIARLGATARYLTSVC
                     TGSLILGAAGFLRGRRAACHWAWRELLPVFGAIVDEGRIVRDGNILSGGGVTAGIDFV
                     LALIAELRGPRAAEMVQLGLEYAPEPPFNAGRPDTAPAEVLEAVNARTATSRAQRRVR
                     LDAWAAAHCAGHHA"
     gene            2248..3183
                     /locus_tag="AC159B_05215"
     CDS             2248..3183
                     /locus_tag="AC159B_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019453959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HIR3377180.1"
                     /translation="MGTVGFYVAPGFQMLDLAGPAGAFEAANDRLESPAYRLHVLAAD
                     KGAVTSSLGVAITGASLDDAVLDTLVVTGGQIDPLLSAGTLSRIVETSRRCRRVVSVC
                     TGAFALAAAGLLDGRRATTHWRYAARLQREYPSIRVEADRIFCRDGNVWTSAGITAGI
                     DLALALIEEDLGFAIAKGVAQHLVVYHRRYGGQSQFAASVDLAPENDRIAAAIGHARD
                     HIAEALTVERLAEAVGMSLRQFSRAFRAQTGTTPARVVERLRADLARGRIETTAEPVE
                     RIADAVGFGDAENMRRAFIRIYGQPPQSIRRAIRS"
     gene            complement(3206..4057)
                     /locus_tag="AC159B_05220"
     CDS             complement(3206..4057)
                     /locus_tag="AC159B_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3377181.1"
                     /translation="MFQLDLLKGKRILVTGGGTGLGRSMSHRFLELGANVVICGRRED
                     VLEQTAEELTKETGAEIETVRCDVRLPDAVEAMMDRIWQKRPLDILVNNAAGQILAPT
                     HKMSSRAIDAVLGIVLHGSAYCTVAAGRRWIDAGERNKVVMSILTVSALMGAPFTVPS
                     AMAKAGVLAMTKSLAVEWGPRGIRTCAIVPGLFHTEGAWSRLMPKERGGDEELVKTIP
                     LRRVGEHIELANLAAFLVSDGAAYINGDAVVIDGGKMQSGGGGASTQSMLDWTDEQWE
                     AIRPKKK"
     gene            complement(4065..>4640)
                     /locus_tag="AC159B_05225"
     CDS             complement(4065..>4640)
                     /locus_tag="AC159B_05225"
                     /inference="COORDINATES: protein motif:HMM:NF013777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIR3377182.1"
                     /translation="RSLTAITPKWLSARLRTLEADGIVTRDVTGEREVRYRLTPKGEA
                     LEPVFDALLVWGMDYALGSPRHGEAIHPGRAIDATVRYLNRRRVRAARRVVWVILFGE
                     DRSYTIRFDGARWSRQRGAAPADVTVQTSPPEWVKFLSADPKGRRRWLRQQRAAGTRQ
                     RLDELMTSFGVLQQTLRSREPGSQRAAMAKN"
BASE COUNT          834 a         1510 c         1464 g          832 t
ORIGIN      
        1 atccggaagc ccggcgcgtc gtcatcgcga ttcgcgacag cggcgtgggg gtcaaggagt
       61 ctgagctcga gcggatcttc gaccgcttcg tgagtagcaa gccccaggga ctggggttgg
      121 ggctggccat cagccggtcg atcgtggagg cccacggagg acatctccgg gcggttcgca
      181 acgacgacca cgggctcacc ttcaccatcg agctgcctgc agatggcgcg gagaccgtcc
      241 cggccgacgc ctccacgcga tgggccggct gacgcggggt acggcccagg cggtagtccg
      301 gcgccgctcg gcgtgtaaaa cagctacaga gcaggcgatg gcggcgacga gaaggcgctt
      361 cgagaagggg cgctcaggaa atccgaaggg cggcgtccaa gcatcaagga agctatccgg
      421 cgccgccccg atgagtgtat aagccgtgca agagcacaga gtgcacacga tcacgttgtg
      481 aacggtggcc gtgttctcga gtacaacaag gtttcgatgg tgctccggaa actcaaatcc
      541 tagctcacgg gctgccgcca tgccgtcaac aagcatgcgc gcgcgatatt cgaggtcagt
      601 ccatgcgcga gccaccatgt gggcgccgtt cgcggtaatc cacgcatcgt cggcatgctc
      661 agtgaactcg gcaacaaggt ctttcggcat gtcggccttc cgcctgagca cgatttccag
      721 tgcggctggc cgcttctcca gttgcttctc gccaacgtct ttattgccta ctcggcaaag
      781 ggggcttgat gtagcaagtc aaccgaggac acgcgcgtat atcctggaat gtatttgatg
      841 ccgaagtctg cgatgaaatt gtcccttgtt gtactcggat gatttttggc tatgccgcaa
      901 aggcacgtcg tcagcatgtt cttcatgtca agacgaggat aggctgacag gatgattttc
      961 ttgatgtcat cactcagctc ctggtagccg agtccgccgt agtcacacgc gatgccgttc
     1021 tgacagcagg ccacgtcggc gcccttatgc tgcgcaatcg acggagtggt gtggagcgcg
     1081 atcgagtccc aaactacttc tgcacggcgc tcccccatat catgcgaaag ggcaaaggcg
     1141 cggccgatgt ccgctcccac aacctcgaag cgacgatcgg gagcgccacc gcgcgcaagg
     1201 ccgagatcgt gcagaagcac cgccaccgcg accagttcgg catccggagc aagcccgcga
     1261 ctctgtgcca gcttcgcgct atagagccac gagcgcatca catggttgaa aagccaaggc
     1321 tgcgattccg cttctgcgtg ggcgtaggcg tcccgaacgg cgggactgtc gggtagaggc
     1381 agatccaact tccggccgac ggccagcgca ccgctgaagc taggcctttc gcttccactc
     1441 atatctcgct ccatggtcgg ggatgcttgg gacctcaggc atggtgacca gcgcaatgag
     1501 cggcggccca ggcgtcgaga cgcacacgac gttgagcgcg gctcgttgcc gtgcgcgcgt
     1561 tcaccgcctc cagtacttct gccggcgccg tatctgggcg gccggcgttg aagggcggtt
     1621 caggagcata ttccaagccg agttggacca tttccgccgc gcgcggcccg cgcagctccg
     1681 cgataagggc cagcacgaaa tcgatgcctg ccgtgacgcc gcccccggac aggatgttgc
     1741 cgtccctgac gatgcggccc tcatccacga tggcaccgaa gacaggaagc aactcccgcc
     1801 atgcccagtg gcaggccgca cggcggccgc ggagaaagcc ggcagcgccc aaaatgagcg
     1861 acccggtgca caccgaagtc agatagcgcg cggttgctcc gagccgggcg atctctttga
     1921 cgaagaggtc attctcgatc gcagcgaggc agccaccgcc gcctggcacc aggagcacgt
     1981 cgcacgactc gatcgcagcc aggtcgtcca ggttcgaaaa ggtgaggcgg tccgccgtaa
     2041 ccggttcacc tcccagcgaa gccacccgga catgcaggtc gggtgtacgc gagagaaact
     2101 ggtgcggccc agtgaagtcg agctgggtca cgccctcgta caccggcacg actacgctga
     2161 ttgcctgtga catcgccgat cctccttggg gaaaagctaa ggctgggact atttgaccgc
     2221 aaggtcatac ttccctatca ttcggccatg gggacagtgg gcttttacgt tgccccaggc
     2281 tttcagatgc tcgacctcgc cggtccggcc ggcgccttcg aagcggcaaa tgaccgcctc
     2341 gaaagtcccg cctatcgact ccatgtcctt gcggctgata agggtgccgt gacaagcagc
     2401 cttggcgtgg ctattactgg tgcgtcgctc gatgatgccg tgctcgatac gctcgtcgtc
     2461 accggcggac agatcgatcc gctcttatcc gcgggcacgc tgagccgtat tgtggagaca
     2521 tcgcgccgat gccggcgcgt tgtgagcgtc tgcaccggtg cattcgccct cgcggcggca
     2581 ggattgttgg acgggcgtcg agcgacgacg cactggcgat atgcagcgcg ccttcagcga
     2641 gagtacccat ccatccgggt cgaggcagat cgcatcttct gtcgggacgg caacgtatgg
     2701 acgtcagctg gcatcacggc cggcatcgat ctcgcgctgg ctttgatcga ggaggacctt
     2761 ggttttgcca tcgccaaagg cgtggcacag cacctcgttg tctaccatcg ccgctatggc
     2821 ggacaatccc aattcgcagc ttccgtcgat ctggcgccag agaatgaccg gattgccgct
     2881 gccattggcc atgctcgtga tcacatcgcc gaggcgctca ccgtcgagcg tttggcggag
     2941 gcggttggca tgagcctgcg ccagttcagc cgcgcattcc gggcacagac tggcaccaca
     3001 cccgcgcgtg tggtggaacg gctccgcgcc gatctggccc gaggccgcat agagacgact
     3061 gccgaacctg tcgaacgcat cgcggacgcg gtgggcttcg gtgacgccga aaacatgagg
     3121 cgggcattca tccgcatcta cggacagccg cctcaatcga tcaggcgcgc aattagaagt
     3181 taggagcgcc cgaacatcac gatactcact tcttcttcgg ccggatcgcc tcccattgct
     3241 cgtcggtcca gtcgagcatc gactgcgtgc tggcaccgcc accgcccgac tgcatcttgc
     3301 cgccatcgat caccacggcg tcgccgttga tgtaggccgc cccgtcgctc acgaggaagg
     3361 ccgcaaggtt cgcgagctcg atgtgctcgc ccacgcggcg cagcgggatc gtcttcacga
     3421 gctcctcgtc gccgccgcgt tccttgggca tcagccgact ccacgctccc tccgtgtgga
     3481 agaggccggg cacgatggcg caggtgcgga ttcctctggg cccccattcg acggcgaggc
     3541 tcttggtcat ggcgagcacg cccgccttgg ccatcgccga cggcacggtg aagggcgcgc
     3601 ccatcagcgc cgatacggtg aggatcgaca tcacgacctt gttgcgctcg cccgcgtcga
     3661 tccagcgccg gccggcggcc accgtgcaat aggccgagcc gtgcagcacg atgccgagca
     3721 ccgcgtcgat ggcgcgtgag gacatcttgt gcgtcggggc caggatctgg cctgcggcgt
     3781 tgttcacgag gatgtcgagc gggcgcttct gccagatgcg gtccatcatc gcttcgaccg
     3841 cgtccggcag gcgcacgtcg caccttacgg tctcgatctc agcaccggtc tccttggtca
     3901 gttcctcggc cgtctgctca agcacgtcct cgcgccggcc acagatcaca acgttcgcgc
     3961 cgagctcgag gaagcggtgg ctcatcgagc ggccgagtcc cgtgccgcca ccagtcacca
     4021 gaatgcgctt gcccttcagc aggtcgagtt gaaacatcgc atcctcaatt cttggccatg
     4081 gccgctcgct gacttccagg ttcgcgtgag cgcaacgtct gttgtaggac tccgaatgac
     4141 gtcatcagct cgtcgagacg ctgccttgtt cccgcggcgc gctgttgccg cagccagcga
     4201 cgtcggccct tggggtccgc gctcagaaac ttcacccact ccggcgggga ggtctgcacg
     4261 gtgacgtcgg ctggtgccgc tccgcgctgt cgcgaccatc gagctccatc gaagcgaatg
     4321 gtgtaactgc gatcctcccc gaagaggatg acccacacga cgcggcgcgc ggcacgtacc
     4381 cggcggcgat tcagatagcg tacggttgcg tcgatcgcac ggccgggatg gatcgcctca
     4441 ccgtgacgtg gcgagccgag cgcatagtcc atgccccaga cgagcaacgc gtcgaacacc
     4501 ggctcgaggg cttcaccctt cggcgtcaac cggtatcgaa cctcgcgctc gccggtgacg
     4561 tcccgggtga cgatcccgtc cgcttcgagg gtacgaaggc gcgcgctcag ccacttcggc
     4621 gtgatcgccg tgagcgaccg
//
LOCUS       DBNGSB010000181         4085 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3811572, whole genome shotgun sequence.
ACCESSION   DBNGSB010000181 DBNGSB010000000
VERSION     DBNGSB010000181.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4085)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3811572"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="AC159B_05230"
     CDS             <1..734
                     /locus_tag="AC159B_05230"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HIR3377183.1"
                     /translation="GLLAGMNRPEVSVYRRPHVGVIVTGDELVAPGQSISGSQIYDMN
                     TYTLSGMIDEAGGVPQPHGILGDNLAVLADRVRMAHAASDVLIITGGSSVGEKDVVAD
                     AIAALGAPGIVVHGIAIRPGKPTILAVCNGKPVFGLPGNVVSAMVTFDQFVRPVIQGL
                     AGLREERRFGLVINARLTQTVSAGGREDHVRVALEERDGAIWASPLPSGSALITSMVR
                     ADGIIVVPMNTAFVEGTEVEVRLIG"
     gene            727..1254
                     /gene="moaC"
                     /locus_tag="AC159B_05235"
     CDS             727..1254
                     /gene="moaC"
                     /locus_tag="AC159B_05235"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HIR3377184.1"
                     /translation="MAKRTSANAQTRKRANSLSHLDAAGRAKMVDVSAKPTTVREAVA
                     RGEVRMRTKTLELIRKNQMAKGHVLAVAQVAGVLAAKRADEFIPLAHPLPLSGIDITF
                     STNGRASAIGIEARVRTAAQTGVEMEALAAVAAAALAIYDMCKAADREMTIGRIRLVQ
                     KSGGRRGDYRRKGEG"
     gene            1251..1754
                     /locus_tag="AC159B_05240"
     CDS             1251..1754
                     /locus_tag="AC159B_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020491797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HIR3377185.1"
                     /translation="MTADLLGVRVAVLTVSDSVSGGRSKDLSGEVIAKTISASGADVV
                     ARDVLPDEQSRIAAKLKSYADELRVDLVLTTGGSGVAPRDVTPEATLEVVERLVPGLV
                     EAARIQTLQKSSMAMLSRSAAGIRARTLIVNLPGSPKAVEEWLEILLPVLRHAVDLLR
                     GTSTGHP"
     gene            1751..2140
                     /locus_tag="AC159B_05245"
     CDS             1751..2140
                     /locus_tag="AC159B_05245"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR3377186.1"
                     /translation="MRPADHCFVCGKDNPIGLKLKFVRQGEGVRAEFSPSALHVGYDG
                     VVHGGIIASLIDDALANVWFVRGQEALTAKIEVRFRHEIQPGDRLIISAYPTGPKAGL
                     MTARAEVARTDGVVVAEGSGFLTVKPA"
     gene            2137..2412
                     /locus_tag="AC159B_05250"
     CDS             2137..2412
                     /locus_tag="AC159B_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3377187.1"
                     /translation="MIFRWLFLPVMVVALAVVVRQHADYLRRRRSVTFRAFLETAGWL
                     VITLASAAAYWGGLESPGTRVVEVSVLVIGLLFVAIGSHARPTADPG"
     gene            2409..2888
                     /gene="smpB"
                     /locus_tag="AC159B_05255"
     CDS             2409..2888
                     /gene="smpB"
                     /locus_tag="AC159B_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018703025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HIR3377188.1"
                     /translation="MSTEQDNERLIATNRKARHDYHIEESVEAGLVLTGTEVKSLRTG
                     RANLQDAFARVEASEVWLYHLHIPPYDSGGYANHEPLRRRKLLLHRTEIQRLYGKAQQ
                     KGYTLVPLKLYFKRGVVKIELALARGKREYDRREEIAKRDADRQIQRAVRGRPSSTR"
     gene            3023..3838
                     /locus_tag="AC159B_05260"
     CDS             3023..3838
                     /locus_tag="AC159B_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3377189.1"
                     /translation="MTTLLALIFSAATTIIIAVLGHQFHFAHFHLIGGIPVGAMAIGA
                     GAAVGIAIAIRLTNNYDTDDFRMLGYVAGFLAYGLVMFADYTTLQLRVGGRVLPAPEF
                     MNVARYFMFLVEEGAKSTVAALPKWVHVPQQIMFWLGMVRLAVESVGVLVATGWSLSL
                     LTGVPFCVRNRRFYELKQVIHSANTDAVREWELAVHQRRPIEARAILARVRAGRPQLQ
                     DRVWKRIVIHQCPICLAGRVRIEKCKRGPGYIRSEHEDEFLLDEARGSALLAT"
     gene            3925..>4085
                     /gene="ssrA"
                     /locus_tag="AC159B_05265"
     tmRNA           3925..>4085
                     /gene="ssrA"
                     /locus_tag="AC159B_05265"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          805 a         1211 c         1293 g          776 t
ORIGIN      
        1 tcggcctcct cgcgggcatg aaccgtcccg aagtctcagt ctatcgccgt ccgcatgttg
       61 gtgtgatcgt caccggcgac gaactggtgg ctccagggca gtcgatcagt ggcagtcaga
      121 tctacgatat gaacacctac acgttgtccg gcatgatcga cgaagcaggt ggggttcctc
      181 agcctcacgg gatcctgggt gataacctgg ccgtgctcgc ggatcgcgtc agaatggcgc
      241 atgccgcttc tgacgttctg atcattaccg ggggcagctc agtgggtgaa aaggatgtcg
      301 tcgccgatgc cattgcggct ctcggggcgc ccggcatcgt cgtgcacggc atcgcgatcc
      361 gtccaggcaa gccaacgatc ctggccgtgt gcaacggaaa gccggtgttt gggctgccgg
      421 gcaacgtcgt ttcagcgatg gtgacctttg accagtttgt ccggccggtc atccagggcc
      481 ttgccggcct gcgtgaggag cggcggtttg gtctcgtgat caatgcgagg ctgacgcaaa
      541 ccgtgtctgc cggagggcgg gaagaccacg tgcgcgtggc gctcgaggag cgcgacggcg
      601 ccatttgggc ctctccgctc ccttcagggt cggccctaat cacgtcgatg gtcagagcgg
      661 atgggattat cgtggtccca atgaacaccg cgtttgtaga aggaaccgaa gttgaggtga
      721 gactgattgg ctaaacgaac atctgcaaac gcgcaaacgc gcaaacgcgc gaactcgtta
      781 tcgcaccttg atgcggcggg tagggcaaag atggtcgacg tctccgccaa acccacgaca
      841 gtgcgggaag ccgtcgcgcg cggcgaagtg cgcatgcgca cgaagacact ggaattgata
      901 aggaagaacc agatggcgaa aggccacgtg ctcgcggttg ctcaggtcgc cggcgtcctc
      961 gccgccaagc gggcggacga attcattccg ctggcacacc cgctgcctct cagcggcatc
     1021 gacatcacgt tcagtacaaa tggtcgcgcc agcgcaatcg gtatcgaggc cagagtgcgt
     1081 acggcagccc aaacgggcgt cgaaatggag gcgctcgccg cggtcgccgc ggccgcgctc
     1141 gcgatctacg acatgtgcaa agccgccgat cgtgagatga ccatcggtcg aatccggctt
     1201 gtgcagaaat ccggcggccg gcgcggcgac tatcgccgca agggtgaagg gtgacggccg
     1261 acctcctggg cgtgcgggtc gcggtcctga cagttagcga ttcggtatcg ggcggacgat
     1321 ccaaagacct cagcggggag gtaatcgcaa aaaccatcag cgcgtcgggc gcggatgtcg
     1381 tggcgcgcga tgtgctgccc gacgagcagt cacggatcgc tgcgaaactc aagagctacg
     1441 ccgatgagct ccgcgtcgac ctggtcctca cgaccggggg aagcggggtg gcgccgaggg
     1501 atgtaacgcc cgaggcaacc ctggaagtgg tggagcggct cgtccctggt cttgtcgagg
     1561 cggcgcggat tcaaacgctg cagaaatcgt caatggcgat gctgtcacgg agcgccgccg
     1621 gcatccgagc gcggacgctc attgtcaacc ttccgggaag cccgaaagct gtcgaagaat
     1681 ggctcgagat tcttcttccg gtgttgcgac atgctgtcga tctgctccgc gggacttcaa
     1741 ccggccaccc atgagaccgg ccgaccactg cttcgtgtgc gggaaagaca accccatcgg
     1801 attgaagttg aagtttgtgc ggcaaggcga gggtgtgcgc gcggagtttt cgccgtcggc
     1861 gcttcacgtc ggatatgacg gcgttgtaca cggcggcatc atcgcatcgc tcatcgatga
     1921 cgcgctcgcg aatgtctggt tcgtccgcgg gcaagaggcg ctcactgcta aaatagaagt
     1981 ccgcttccgc catgagatcc aaccgggcga ccggctgatc atttccgctt acccgacagg
     2041 cccgaaggcc ggactgatga ccgcgcgggc agaggtggcg cggaccgacg gcgtcgttgt
     2101 cgccgaaggc agcggttttc tcactgtgaa gccggcgtga tctttcgctg gctcttcctg
     2161 cctgtcatgg tggtggcgct cgcagtagtc gtccgacagc acgccgatta tctgcgccgt
     2221 cggcggtccg tcacgttccg cgcgttcctt gagacggcag ggtggcttgt cattacatta
     2281 gcgtccgcgg cagcatactg gggcggactt gagagccctg gcacgcgtgt cgtcgaggta
     2341 tctgttcttg tgatcgggct gctgttcgtc gccatcggat ctcacgccag gccgaccgca
     2401 gatccgggat gagcaccgag caagacaatg aacgattgat cgcgacgaat cgaaaggccc
     2461 gccatgacta tcacattgag gagagcgtcg aagcaggatt ggtgttgacc gggaccgagg
     2521 tgaagtcgct gcggaccgga cgagcgaacc ttcaagacgc gtttgctcgg gtcgaagcga
     2581 gcgaagtctg gctctaccac ctgcacatcc ccccgtatga ttccggcggg tatgccaacc
     2641 acgagccgct tcgccgtcgg aagctgctct tgcaccggac tgagatccag cggctctacg
     2701 gaaaggccca gcaaaaaggc tatacgctgg ttccgctcaa gctgtacttc aagcgagggg
     2761 tggtcaaaat cgagctcgcc ctggcccgcg ggaaacgcga gtacgatcga cgcgaggaaa
     2821 tcgccaaacg ggatgcggac cgccagattc agcgggccgt ccgggggcgg ccctctagca
     2881 cccgttaggt ccccactacc actaacccgg caccaagagt cacgccctga agtcaagcaa
     2941 cagtaccttt ccctccacag aaccgcgact gaaactggag gccctagggc atgtgctttg
     3001 ggagtaggga ggagggcgcc ctgtgaccac tctactcgcg ctaattttca gtgcggccac
     3061 cacgattatc atagccgtgc tgggtcacca gtttcacttc gcccattttc acctcatcgg
     3121 cggtatcccc gtgggtgcaa tggccatcgg cgccggcgct gccgtgggta tcgcaatcgc
     3181 aatcaggctc acgaacaact acgataccga cgacttcagg atgctcggct atgttgccgg
     3241 attcctcgcc tatggcctgg tcatgtttgc cgactataca acactccagt tgcgcgttgg
     3301 agggcgtgtc ctgcccgctc cggaattcat gaacgtcgcg cgatacttca tgttcctcgt
     3361 cgaagagggc gcaaaatcta cagtggccgc gctccctaaa tgggtgcacg tcccacagca
     3421 gatcatgttc tggctgggga tggtcaggct ggccgtagag agcgttgggg tgctcgtggc
     3481 cactgggtgg tccctgtctc tccttacagg tgtgccgttc tgcgtgcgca accgccggtt
     3541 ttatgagctc aagcaagtga tccacagcgc caacactgat gcagtgcgag agtgggagtt
     3601 ggcagtccac cagcgccggc ctatcgaagc ccgcgcgatc ctcgcacgcg ttcgggcagg
     3661 ccgaccccaa ctccaagacc gcgtctggaa gcggattgtg attcatcaat gcccgatctg
     3721 tctcgccgga cgagtccgca tagaaaaatg caagcgaggc cccggctata tccggtccga
     3781 acatgaggat gagttcctgc tcgacgaagc cagaggctcc gcgctgctgg cgacctgaca
     3841 gtcgccaatc gcagaatcca tacgatcttc actatctgca atagcaaatg ttacctcatg
     3901 atataatctc tatgtcgaaa agaggggggc gaacggtttc gactgagaga gcagaagtga
     3961 ggttgcgcgt cgaggtcttt ccgggtcctc gttaaacccc tggaaggcga ataaccgcca
     4021 ataaccagta cgcttacgcg gcctaagtag ccgtgtacgc tcacccggga ttgccggcga
     4081 tccgg
//
LOCUS       DBNGSB010000182         4019 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_1053059, whole genome shotgun sequence.
ACCESSION   DBNGSB010000182 DBNGSB010000000
VERSION     DBNGSB010000182.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_1053059"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="AC159B_05270"
     CDS             complement(<1..857)
                     /locus_tag="AC159B_05270"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HIR3377190.1"
                     /translation="MADAAAPATSPPPSVATTVVVREGQSLWDIATARGISVEAVAEA
                     NGLSNAELIRPGQRLVIPPSGASARMQKPIMRTTTKPQVRAARAPARRQQPASGWVVV
                     RQGQTLSEIADQHKTTVRTLVDTNGLRSAHAIRVGQRILIPGRGAGSGGGMAAALLPA
                     SRSQAAVVRVAGFLWPARGVLTSRFGWRWRRHHDGIDIAAPRGTPVHAARAGRVIFSG
                     WYYGYGRAVIVDHGEGLTTLYGHNSTLLVRTGESVTSGQLIARVGCTGRCYGSHLHFE
                     IRVNGRAVNP"
     gene            complement(1256..2518)
                     /gene="rho"
                     /locus_tag="AC159B_05275"
     CDS             complement(1256..2518)
                     /gene="rho"
                     /locus_tag="AC159B_05275"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HIR3377191.1"
                     /translation="MAAIAELEQKTLDELQTLAKELNIANFRRYRKQELIMRILQMQT
                     EQSGLMFRSGILEIMAEGYGFLRHEGYLPGSEDIYVSPSQIKRFGLRVGDEVLGQVRP
                     PKDNEKYYALLRVEAVNGLDPEQARNRPDFERLTPVFPHERVKLESDADLTTRMVDLF
                     APIGKGQRAMIVSPPKAGKTTLLKKIGTAVVTNFPEVYLIVLLLDERPEEVTDMRRSV
                     EAEVVASTFDRPPEEHIQVADLVLERSKRLVEGGRDVVVLLDSLTRFSRANNLVIPPS
                     GRTLSGGLDPAALHRPKRFFGAARKIEEGGSLTIVATALIDTGSRMDDHIYEEFKGTG
                     NMELHLNRKLQERRTFPAIDIKMSGTRREELLLTEDELRKVWVLRKSLEQLDTVAMTE
                     LILDRLRKTTSNQQFLKSIVKSAEEDLQ"
     gene            complement(2589..3260)
                     /gene="fsa"
                     /locus_tag="AC159B_05280"
     CDS             complement(2589..3260)
                     /gene="fsa"
                     /locus_tag="AC159B_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012310648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HIR3377192.1"
                     /translation="MKFFLDTANLEEIRNGARWGLVDGITTNPSLAGKEGMEYRDLIR
                     EIAKIVSGPISVETTSNDPKEMVTQGRDYVTWSQNVVVKVPATAEGITAAMQFRDLGI
                     RTNVTLTFSVNQAILAAKAGAFFVSPFVGRLDDAGHDGMDVVRETVQVYRNYGFKTQV
                     LASSLRHPLHVTQAALAGADIATLPFKVMEQLFKHPLTESGQAKFLADWRKLQTELEK
                     KKTKV"
     gene            complement(3268..>4019)
                     /locus_tag="AC159B_05285"
     CDS             complement(3268..>4019)
                     /locus_tag="AC159B_05285"
                     /inference="COORDINATES: protein motif:HMM:NF024102.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HIR3377193.1"
                     /translation="FIIKDTQGSEIARWSLGRAFLPLTEPVTLAAGAKFEYKTRWRQL
                     DQNDDPVPPGRYEITAIQTTKGNPTVLALAFHRGVLVVFPDNTFRPKAEVTRAELAAA
                     SVKAAGLSETPGTPNVIDAAEIPPALRGAVATAIEKRLVLVGADRAFRPSRPATRADV
                     ALALDVLIESLKRYDFSKGTLKDLRVGSPTIIQIEDEQKALRTFRVARAHAVYRNNVA
                     AELKDIKVGDQLQFLKAGDVGDVAYIEATAR"
BASE COUNT          702 a         1263 c         1260 g          794 t
ORIGIN      
        1 gggttgactg ctcttccgtt gacgcggatt tcaaaatgca ggtgtgaccc gtagcagcgg
       61 cctgtgcatc ccacgcgggc gatcaattga ccggaggtca cactctcacc agtgcgcacg
      121 agcagcgtgg agttgtgccc atacaaggtc gtcagccctt cgccgtggtc gacaatgacg
      181 gcgcgcccgt aaccgtagta ccagcccgag aagatgacgc ggccggctct cgctgcatgc
      241 accggcgtgc ctcgcggcgc ggcgatatcg attccatcat gatgccggcg ccaacgccac
      301 ccgaaccgag acgtcaggac gccgcgggca ggccacaaga acccggccac cctcactact
      361 gccgcctgac ttcgacttgc tggaagcagc gcggcagcca tgccaccgcc ggaaccagct
      421 ccccggccag ggatgagaat acgctgaccc actcgtatcg cgtgggcgct gcgcagaccg
      481 ttcgtgtcga caagggtgcg gaccgtcgtc ttgtgctggt cggcgatctc ggaaagggtc
      541 tgcccctgcc tgacgacaac ccaaccagag gccggctgct ggcgtcgggc tggcgcgcgg
      601 gccgcccgaa cctgcggttt ggtcgtcgtc ctcatgatag gcttctgcat ccttgctgag
      661 gcacccgaag ggggaataac aaggcgctgg cccggacgga tgagctcggc gttggaaagt
      721 ccattggcct ccgcgaccgc ctcaaccgag atcccacgag ccgtcgcgat gtcccagagc
      781 gactggccct ctctcacgac gacggtcgtc gcaacagatg gaggcgggct tgtcgccggc
      841 gctgccgcgt cggccacctc gacatgaggt ggcgcgaggt ggaacagtgg ggcgaaggtt
      901 cgagcgacgg agacgtcatt gcgaattgct tgaaggaaag gaacaggtga ggaggaaacg
      961 gccgacgggg gcgcgctgga tgtccaaagc attaggccgc ccagcagggt actaatgccg
     1021 tatagcttgg ccttggagcc catcctccac ccgcctcgcc gaaattcgac tgttacctat
     1081 gcccagggcc tacgagtatt cgccccagta tatcaaactc ctgccccgct ctacgcattg
     1141 aaatgccgcc tatcctgcgc aaagttaggg cgtgggcgcc cctgagctta tcttccctat
     1201 gcccgaagga gggtccggag atacccggat tggaggatac tgctaggaat ctcggctact
     1261 gaagatcttc ttcagcgctt ttcacgatgg acttgaggaa ctgctggtta ctggtcgtct
     1321 tccggagccg gtccaggatg agctcggtca tcgcgacggt gtccagctgc tccaggctct
     1381 tccgcaggac ccagacctta cgcagctcgt cctcggtaag caggagctct tcccggcgcg
     1441 ttccgctcat cttgatatcg atggccggga aggtacggcg ctcctggagc ttccggttca
     1501 ggtgcagctc catgttaccg gtgcccttga actcctcata gatgtggtcg tccatcctgc
     1561 tgcccgtatc gatgagcgct gtggcgacga tggtcaggct gccgccctct tcgatcttcc
     1621 gcgccgcgcc aaagaaccgt ttgggccggt gcagcgccgc agggtcgaga ccgcctgaga
     1681 gcgtgcggcc ggagggcggg atcacgaggt tgttggcgcg ggagaaacga gtgaggctgt
     1741 ccagcaggac gaccacgtcg cgaccgccct cgaccaggcg cttggaccgc tccaggacga
     1801 ggtcggccac ctgaatgtgt tcttctggag gtcgatcaaa ggtcgacgcc accacttcgg
     1861 cctcgaccga ccggcgcatg tccgtgacct cttccggccg ctcgtccaac aggaggacga
     1921 tgagatagac ctccgggaag tttgtgacca cggccgtacc gatcttcttc aggagcgttg
     1981 tcttgccggc ctttggcgga gatacgatca tcgcccgttg ccctttgccg atcggggcga
     2041 acaggtccac catcctggtc gtgaggtccg cgtcgctctc cagcttgacc ctctcgtggg
     2101 ggaacaccgg cgtcagccgc tcgaagtccg ggcggttgcg ggcttgttcg ggatcgagtc
     2161 cgttgacggc ctccacccgc aacagcgcgt agtacttctc gttatccttg ggcggccgga
     2221 cctgcccgag cacttcatct ccgacccgga gaccgaacct cttgatctgg gacggggaga
     2281 cgtagatgtc ctcagaaccc ggcagatacc cctcgtggcg aaggaagccg tacccttcag
     2341 ccatgatctc caggatcccc gagcggaaca tcaacccgga ctgttcggtc tgcatctgga
     2401 ggatgcgcat gataagctcc tgcttccgat accggcggaa gttggcgata ttgagttcct
     2461 tcgccagggt ctggagctca tccaaggttt tctgttcgag ttcagcgatg gcggccaagg
     2521 ccgatccacc tccaccaaca atcgattatg gaatgcgtgc acatgggaga tacgatgagg
     2581 tacctaactc acactttggt ctttttcttc tcgagttctg tctggagttt gcgccagtcg
     2641 gccaggaact tggcctgacc ggactcggtc agcgggtgtt tgaacagctg ctccatgact
     2701 ttgaagggca gggtggcgat gtccgctccg gccagcgccg cctgggtcac gtggagcggg
     2761 tggcggaggc tgctcgccag gacctgcgtt ttgaagccat aattccggta cacctggacc
     2821 gtctccctga ccacatccat cccgtcgtgg ccggcgtcgt ccagccggcc cacgaacggg
     2881 ctcacgaaaa acgcgccggc cttcgccgcc aggatcgcct ggtttacgga aaacgtcagg
     2941 gtgacgttcg tgcggatacc gaggtcccgg aattgcatcg ccgcggtgat cccttcagcc
     3001 gttgcgggca ccttgaccac gacgttctgg ctccaggtga catagtcgcg gccctgcgtg
     3061 accatctctt tgggatcgtt gctggtcgtc tcgaccgaga tcgggccgga gacgatcttc
     3121 gcgatctcgc ggatgagatc acggtactcc atgccctctt tgccggcgag tgatggattg
     3181 gtcgtgatgc cgtccacgag tccccaccgt gcgccgttgc gaatctcttc gaggttcgct
     3241 gtatcaagga agaatttcat cgctcaacta ccgcgccgtc gcctcgatgt aggcgacgtc
     3301 gcccacgtcc ccggctttga ggaactggag ctggtcgccc accttgatgt ctttcagttc
     3361 cgcagcgacg ttgttgcgat acacggcgtg cgcgcgcgcg acgcggaacg tccgcagcgc
     3421 cttctgttca tcctcgatct ggatgatcgt agggctgccc acacgcaagt ctttcagggt
     3481 ccctttcgag aagtcgtagc gcttcaacga ttcgatcaac acgtcaaggg caagcgcgac
     3541 gtcggcgcgc gtggcgggac gggatggacg gaaggcccgg tccgcaccga cgaggacgag
     3601 acgcttctcg atcgccgtcg ctacggctcc ccgcagagcc ggcggaatct ccgcggcatc
     3661 tatcacgttc ggcgtgcccg gcgtctcgct caggccggcc gccttcacgc tggctgcggc
     3721 cagctcagcg cgcgtgactt ccgcctttgg acggaaggta ttgtcgggga acaccacgag
     3781 cacaccgcgg tggaacgcga gcgccaggac ggtcgggttg cctttcgtcg tctggatcgc
     3841 ggtgatctcg taccgtccgg gtgggacggg gtcgtcgttc tgatcgagct gccgccatcg
     3901 ggtcttgtac tcgaacttcg cgccggcggc cagcgtgact ggctcggtaa gcggaagaaa
     3961 cgcgcgaccg agggaccatc gtgcgatttc ggacccctgc gtatctttga tgatgaaat
//
LOCUS       DBNGSB010000183         4610 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3505621, whole genome shotgun sequence.
ACCESSION   DBNGSB010000183 DBNGSB010000000
VERSION     DBNGSB010000183.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4610)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3505621"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="AC159B_05290"
     CDS             <1..876
                     /locus_tag="AC159B_05290"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR3377194.1"
                     /translation="HPFVVYGDAEGPSRALDEAKRQLGTPRRIGAGDVMRADALLHLQ
                     RLWPDADYVVASDILGPLRMRKSAEEIALLKRAAATADRAFEAAVAASRSGASETELS
                     QAAVGGFHAAGASEGTSSIVGSGPNSAFPHHHTSARRIEAGEPVLFDLGSKLDGYWSD
                     LTRMAYLGKPAARYTEIHRIVDFAVRAALAVITPGARIRDVDLAARDVIKTAGYEQYF
                     THRTGHGIGLSVHELPSITHTNDVPLEAGMTFSVEPGIYLSGEFGVRLEEIVAVTATG
                     HEVLSRLSRDVRVIG"
     gene            complement(866..1819)
                     /gene="menA"
                     /locus_tag="AC159B_05295"
     CDS             complement(866..1819)
                     /gene="menA"
                     /locus_tag="AC159B_05295"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: protein motif:HMM:TIGR00751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HIR3377195.1"
                     /translation="MTTSATDEGTALPRFRDSAFPRPAWPAVWWRALRPHSFTASVTP
                     VFVGAAAAFHDGHLDAVPFLVTLVAAVAIHAGTNLANDYYDHVRGVDALESPGPSGVI
                     QQGLLPARQVLTAALLAFAVGGVLGLYLVSIRGWAVLLLGLLGVLAGFAYTGGPLPLG
                     YVGLGDFVVFAFMGMAIVLGTFFVMTGGLSATAVWAALPVAALVTAILVVNNLRDIDE
                     DRAAGKRTLATFLGEKATRIEFLLLIVAAYVAVVAGIVFGALPYQAGVVFVTLPRTVR
                     LWQAVRTEADRRQLTMMGLRGSARLHLNFGLLLAAAFLVSR"
     gene            complement(1855..2610)
                     /locus_tag="AC159B_05300"
     CDS             complement(1855..2610)
                     /locus_tag="AC159B_05300"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3377196.1"
                     /translation="MSRPSYRWLAVVSPLALLLVWEILSQTGVVDPRFFPPPSTIVRT
                     LASMIRTGELPFHIGVSLRRILIGFVLGSIPAVLLGLAMGLARPVRALFMPIVAAIYP
                     IPKIAIYPLIIFYLGIGEASKVTIVALSIFFLVLLNTMAGVLGLDRAYFNIARAYGAG
                     ARSIFTTVAMPGAMPQIFTGLKLAMGFALIVIVGAELLGSDAGIGFLIWRSYQIFAID
                     AMYVGLLVTAILGWLATIALDWLERLAMPWRPN"
     gene            complement(2607..3404)
                     /locus_tag="AC159B_05305"
     CDS             complement(2607..3404)
                     /locus_tag="AC159B_05305"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3377197.1"
                     /translation="MIAAAPPRVAPITVRGVSKVYPARHGSFVALRDVSFSVAPGEFC
                     AIIGPSGCGKTTLLHILAGLIPATSGTVEVPPHERGRLVTALIFQGISTLPWLTVQQN
                     VEYGLRHMLVPPEERRARSVEQLRRVGLDRFVDAFPHQLSEGMRQRVSIARALATDPD
                     VLLMDEPFANLDEQNRLLLQEELLRLWQENRQTVLFVTHSLDEAIRLSDRVLVMTSAP
                     GRIKSEVPVRLARPREFRSVRQDPEYGELSARLWDDLREEVLGARQI"
     gene            complement(3401..4423)
                     /locus_tag="AC159B_05310"
     CDS             complement(3401..4423)
                     /locus_tag="AC159B_05310"
                     /inference="COORDINATES: protein motif:HMM:NF024772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3377198.1"
                     /translation="MIRRLMVLSFVIAMATMVAAPGQVAGQPQVTTLRVVHVPVLIFA
                     PLYVAIERGYFARQGIEIELITTPGGVSSFAVLAGGRAEVVVGGLGAALFNAAARDLD
                     FKVVGPVHMERPPVSTPLVVSKKAWDSGEIRSIRDLRGKRVSTNVLGSATEYWVQSAL
                     LKGGLTIDDVQLVAVNFPEVPAALANGAIAAGLLGEPLATLAEEQGQIVRLSEDFVNG
                     VQVTALYYSGEFMRAQPKLALGFMVAWLQASRDLFNDGYKRDDVAAIVERYTRVPAPI
                     VKRARPPFHEPNGQMNFNDFKRLQEFFKRRGHLTYEKLLEPKQYIDTSYVREALRILG
                     PFKLSK"
     gene            complement(4420..>4610)
                     /locus_tag="AC159B_05315"
     CDS             complement(4420..>4610)
                     /locus_tag="AC159B_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3377199.1"
                     /translation="LDETRARDELKGVAPEFTRHFTNPEYPVLVIKIDEITFVDRPGG
                     IYPRQHLELRDGEWRFAQ"
BASE COUNT          939 a         1487 c         1414 g          770 t
ORIGIN      
        1 catccgttcg tggtctatgg ggacgctgag gggccgagcc gtgcgctcga tgaagcgaaa
       61 cgacaactcg gcacgccccg gcgcatcggc gcgggcgacg tgatgcgggc cgacgccctg
      121 ctgcacctcc agcgactatg gcccgacgcc gactacgtgg tggcgtcaga tatcctgggg
      181 ccgctcagaa tgcggaagtc ggccgaggaa attgcgttgc tcaagcgggc cgctgcaacc
      241 gccgaccgag ctttcgaagc cgcggtcgcg gcgagcaggt cgggcgcctc cgagacggaa
      301 ttgtcgcagg ccgcggtcgg cggcttccat gcggctggcg cgagcgaagg cacatcttcg
      361 atcgttggat ctggtccaaa cagcgcgttc ccgcaccacc ataccagcgc gcggcgcatc
      421 gaggccggcg agcctgtgtt gtttgacctg ggaagcaaac tggatgggta ctggtctgac
      481 ctcacccgga tggcgtatct tggaaagccg gctgcgcggt ataccgagat ccaccgcatc
      541 gtcgactttg cggtacgcgc ggcgctcgcg gtaatcacac ctggtgcaag gatccgcgat
      601 gtcgatctgg cagcgcgcga tgtgatcaag acagcgggat acgagcaata cttcacgcac
      661 cgcaccgggc acggcatcgg cctgtctgta cacgaactgc cttccatcac gcatacgaac
      721 gacgtgccgc tcgaggccgg catgacgttc agcgtagaac ctgggatcta tctatcgggc
      781 gaattcggcg tgcgtctgga ggagatcgtt gccgttacag cgaccggaca tgaggtgttg
      841 agccggttat cgcgagacgt gcgagtcatc ggctgaccag gaacgccgct gccagcagca
      901 gaccaaaatt caagtgcagt cgtgcagacc cccgcagacc catcatcgtg agctgtcgtc
      961 gatccgcttc cgttcgaact gcctgccaca ggcgcactgt ccgtgggagc gttacgaaca
     1021 cgaccccggc ctgataggga agcgcaccga aaacaatccc ggcaaccacg gcaacgtacg
     1081 cggcgacgat taataggagg aactcaatcc gtgttgcttt ttcacccagg aacgtggcca
     1141 gcgtccgctt gcccgcggcg cgatcctcgt cgatatctct gagattattc acgacaagga
     1201 tggcggtcac gagcgctgcc accgggagcg ccgcccaaac tgcagtcgcc gagaggcctc
     1261 cggtcatgac aaaaaaagtc cccagaacga tcgccatgcc catgaaggca aacaccacga
     1321 agtcgccgag ccctacatac ccgagcggga gcggcccgcc cgtatacgca aacccggcga
     1381 ggacgcccag gaggcccagc aacagaaccg cccatccccg gatgctcact agatacaacc
     1441 ccagcacccc gccgacggcg aacgccagca gtgctgctgt taagacctgc cgcgctggta
     1501 atagcccttg ctggatcacc ccgctgggac ccggcgactc aagtgcgtcg acaccgcgga
     1561 cgtggtcata gtaatcgttc gcgagattcg tgcccgcgtg aatcgccacc gccgcgacaa
     1621 gtgtgacgag aaacgggact gcgtccaggt ggccatcgtg aaaggcggcc gcggccccaa
     1681 cgaaaaccgg cgtgaccgat gcggtgaacg agtgggggcg gagggctctc caccatacgg
     1741 ccggccaagc tggccgtgga aacgcggaat cgcggaaacg cggtaacgcg gtgccctcat
     1801 cagtcgcgga cgtcgtcacc cgcgtttccc cgcttccctg tttcctcgtt tccgtcagtt
     1861 aggtctccac ggcatggcaa gccgctcgag ccagtcgagg gcaattgtcg cgagccaacc
     1921 gagaatagcc gtcaccagca gcccgacgta catggcgtca atcgcaaaga tctgatatga
     1981 ccgccagata aggaatccaa tgcccgcatc cgaccccagc agctccgcgc caacgatgac
     2041 gatcagcgcg aaccccattg ccagcttcaa cccggtaaag atctgcggca tcgctcccgg
     2101 catggccacg gtggtgaaga tgctgcgcgc tccggctccg tacgccctgg cgatattgaa
     2161 ataagcgcgg tccaacccca gtacgccggc catcgtgttg agcagcacca gaaagaaaat
     2221 gctcagtgcg acgatggtca ccttcgatgc ctcgccgatc cccagataaa aaatgatcaa
     2281 agggtaaatg gcgatcttcg gaatgggata gatcgcggcg acgatcggca tgaagagtgc
     2341 ccggaccgga cgcgcgagtc ccatggccag gcccaggagc acggctggaa tcgatccaag
     2401 gacgaatccg atcaggattc tccgcaggct cacgccgatg tggaatggga gctcgccggt
     2461 tcgaatcatg gacgcgagtg tccggacgat tgtgctcggg ggcgggaaga accgcgggtc
     2521 gacgacgcct gtttggctca ggatctccca gaccagcagt agcgccagcg gcgagacgac
     2581 tgcgagccaa cgatatgagg gtctgctcat atctgtctcg ccccgagcac ttcctcccgc
     2641 aggtcatccc atagcctcgc gctgagctcg ccgtactcgg gatcctgacg caccgatcga
     2701 aactcacgcg ggcgtgccag cctgaccggc acctcagact tgatccgacc gggcgcggag
     2761 gtcatcacga ggacgcgatc tgagagacgg atcgcttcat caaggctgtg cgtgacgaag
     2821 agaaccgtct gccgattttc ctgccagagg cgcagcaatt cctcctggag caggaggcgg
     2881 ttctgttcgt cgaggttcgc aaagggctca tccatcagca ggacatcggg gtcggtggcc
     2941 agcgcacggg cgatgctcac gcgctggcgc atcccctcgg agagctggtg cggaaaggcg
     3001 tcgacgaatc tatccagtcc gacccggcga agttgctcga ccgaccgggc ccggcgttcc
     3061 tccggcggga cgagcatgtg ccgcagcccg tactcgacgt tctgctgcac ggtaagccag
     3121 ggcagagtgc tgattccctg aaaaataagc gcggtgacca gccgtccgcg ttcgtgcgga
     3181 ggcacctcaa ccgtgccgct ggtggcgggg atgagcccgg ccaggatgtg cagcaacgtc
     3241 gtctttccgc agcccgacgg cccgatgatc gcgcagaact cccccggcgc cacagaaaac
     3301 gagacgtcgc gcagcgccac aaacgacccg tggcgggccg gatacacctt gctgacgccg
     3361 cggacggtga tcggcgcgac ccgcggtggg gcggcagcga tcatttactc agcttgaacg
     3421 ggcccagaat cctgagcgcc tcgcggacgt aagaggtatc gatgtactgt ttcggttcca
     3481 gcagcttctc gtacgtcaag tgcccgcgcc gcttgaagaa ctcctggaga cgtttgaaat
     3541 cattgaagtt catctgaccg ttgggctcgt gaaaaggtgg cctcgctctc ttcacgatcg
     3601 gagccggcac gcgcgtgtac ctctcgacaa ttgccgcgac gtcgtcgcgc ttgtatccgt
     3661 cgttgaacag atcccggctg gcctgcagcc acgcaaccat gaatccaagc gcgagttttg
     3721 gctgcgcgcg catgaactct ccgctgtaat acagcgccgt tacctggacg ccgttgacaa
     3781 aatcctcact gaggcgcacg atctgcccct gttcctcagc caacgtcgcc agcggctcgc
     3841 ccagcaaccc cgccgcaatt gcgccgttcg ccaatgccgc ggggacctcg gggaagttca
     3901 cagccacgag ctgaacgtcg tcgatcgtga gcccaccctt gaggagcgcg gactgcaccc
     3961 agtactcggt cgccgacccc agcacgttgg tcgagactcg cttcccgcgg agatctctaa
     4021 tagagcgaat ctcaccactg tcccaggctt tcttgctgac aacgagaggc gtgctgaccg
     4081 gaggacgctc catgtgcacg ggaccgacca ctttgaagtc gagatcccgc gctgccgcat
     4141 tgaatagcgc ggcgccgagc ccaccgacga cgacctcggc acggcctcct gcgagcacgg
     4201 cgaatgacga cacgcctccg ggcgtggtga tcagttcaat ctcaattccc tggcgcgcga
     4261 agtatccgcg ctcaatggcc acgtacagcg gcgcgaaaat cagcaccggc acgtggacga
     4321 cccgcagtgt cgtcacctgg ggctggccgg cgacttgccc gggcgcggcg accatcgtcg
     4381 ccatcgcgat cacaaacgac agaaccataa gtctgcgaat cattgggcaa acctccactc
     4441 cccgtcccgt agctcgaggt gctgacgcgg atagattccg ccgggacggt cgacaaacgt
     4501 gatttcatcg atcttaatta ccagtaccgg gtattccggg ttggtgaagt gacgggtgaa
     4561 ctcgggcgca acgcccttca gctcgtcgcg ggcgcgggtc tcgtcgaggc
//
LOCUS       DBNGSB010000184         6112 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3507918, whole genome shotgun sequence.
ACCESSION   DBNGSB010000184 DBNGSB010000000
VERSION     DBNGSB010000184.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 6112)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6112)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6112
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3507918"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1187
                     /locus_tag="AC159B_05320"
     CDS             <1..1187
                     /locus_tag="AC159B_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3377200.1"
                     /translation="TRAGYGKWRRPPSPYDTFMEQEGVPIYRGVGVRRVQDLELAPWG
                     RMGGRGTFIQLFGTEGLWGCYLTEIPAAAALEPERHLYEEVVLVVEGRGALEIWQQEG
                     GKRDLLEWQPGSLFTVPLNTHHRLINAMSSGPALLLVGTTAPLVMNLFGNREFVFDCP
                     FVFRDRYDGGEEYFKPQVRELEPDPHRGLAMVRTNIVPDARNVQLPLDNRRSPGFRRL
                     EPYLDKNVFYQWIGQHESGRYSKAHAHESAAVLICLGGKGYTYAWPREVGTRPWETSQ
                     ANKVVRQDYEPVGMVSAAPMGGQWFHQHFGVAREPLRLMAWFGPFGRGTGREHGRPGE
                     EVLDKNALHLEEGGSSIAYWEEDPYIRREYVGALQREGVEFRMDESFYQRSPGVKTTA
                     TR"
     gene            1184..1888
                     /locus_tag="AC159B_05325"
     CDS             1184..1888
                     /locus_tag="AC159B_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010223624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR3377201.1"
                     /translation="MSKAVLTSKDQALREQVVTASHVLGLEDQGDFIWGHVSIRDPEG
                     RGVWMKATGWGFEEVTPDRVLLIGWDGEVLAGDGRRHLEYPIHTEIMRARSDVNSVVH
                     THASHAVAFASLMEPMRPISHEGAMFVPPDIARFTKTGDLILTRELGQTLAGTLGQRN
                     AALMVHHGIVTAGEDVETSVVTAILLERACQKQLLAMAAGELRTWSSDEEALAKRSRV
                     YSAKQAWAYLVRKIGT"
     gene            1888..2301
                     /gene="pcaC"
                     /locus_tag="AC159B_05330"
     CDS             1888..2301
                     /gene="pcaC"
                     /locus_tag="AC159B_05330"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019926827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HIR3377202.1"
                     /translation="MNSRYETGLKIRKEVLGADHVARSLAEATLDADFQRFITETAWG
                     SVWARPDLDRRTRSLVTIAILAALGREELALHLRASQNVGVDQKEIVEVLLHVAAYAG
                     IPAANAAFAMAKAQFGNNASKAKTGKRARGGRHGG"
     gene            2279..3013
                     /gene="pcaH"
                     /locus_tag="AC159B_05335"
     CDS             2279..3013
                     /gene="pcaH"
                     /locus_tag="AC159B_05335"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019926831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HIR3377203.1"
                     /translation="MEDGTVVRTYSPDFPPYFYEAYRSTVRRSPRQPLIAVPHTLSEI
                     TGPGPAVSGADGEDNDLTQNLGSEGEAVGERIIVTGRVLDDDGNPVPDTLVEIWQANA
                     AGRYRHRIDQHHAPLDPNFLGFGHCRTDKQGVYSFTTVKPGAYPWENHPNAWRPAHIH
                     FSLFGPSWISRLVTQMYFPGDPLHELDPIFNSVPTDAARDRLIANYAHDITKPAWALG
                     YRFDIIVRGPEATPFELPKPARTAKR"
     gene            3010..3582
                     /gene="pcaG"
                     /locus_tag="AC159B_05340"
     CDS             3010..3582
                     /gene="pcaG"
                     /locus_tag="AC159B_05340"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HIR3377204.1"
                     /translation="MTRRGLTPPQTVGPFFHGGMLHRPLNVMIAPETKGERIRIEGHV
                     YDGDRAPVSDAVVEFWQANAAGRYRHPADQRSIPLDQSFVGFGRTGTDEAGTFWFETI
                     KPGAVPFPGGGTQAPHINVAVLARGLLHHLPTRLYFEDDPTVRDDPVLRTIPEKRRAT
                     LLAKRIPGGTPAYQFDIILQGDGETVFFLI"
     gene            3592..4437
                     /locus_tag="AC159B_05345"
     CDS             3592..4437
                     /locus_tag="AC159B_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HIR3377205.1"
                     /translation="MSKAVRFLSLADAMVEYVQDGDVVAMEGFTHLIPFAAGHEVIRQ
                     GKRSLTLIRMTPDLIYDQLIGMGCARKLIFSWGGNPGVGSLHRLRDAVENSWPNPLEV
                     EEHSHAGVSAAYAAGAAGLPLAVLRGYVGTDLPRVNSNIRFIEDPFTGERLAAIPSLR
                     PDVSVIHAQRADRAGNVLLEGIVGVQKEAVLAAKRAVVTVEEIVSDLRPPSPNSVVLP
                     NWTIDAVVEAPGGAKPSYAHGYYKRDNAFYSAWDAISRDRTTFLDWMRTHVLEQGSES
                     HAVQI"
     gene            4421..5200
                     /locus_tag="AC159B_05350"
     CDS             4421..5200
                     /locus_tag="AC159B_05350"
                     /EC_number="2.8.3.-"
                     /EC_number="4.1.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase subunit beta"
                     /protein_id="HIR3377206.1"
                     /translation="MQSKSDYTPPEMMITAAARALKNDDVCFVGIGMPSAACNLARLT
                     HAPDITLIYESGTIGTKPQVLPLSIGDPELCETALTTVSVPEVFGYWLQGGRMTVGFL
                     GAAQIDRFGNINTTVIGPYERPKVRLPGGGGAPEIASYCREVFVIVSQTPRSFVPRVD
                     FITSLGFGNGGDHRQRLGLSTLGPSLVVTDLCLMRPDPETKEFIVTSIHTGVTREEIT
                     QRTGWPVRFSGDVTQTPPPETEELRVLRDLIARSPRGGGAS"
     gene            5197..>6112
                     /locus_tag="AC159B_05355"
     CDS             5197..>6112
                     /locus_tag="AC159B_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HIR3377207.1"
                     /translation="MTDLAGTMFTTPEMSAIFSGSAFVQRMLDVEAALARAHARAGVI
                     PQQDAEAIVSKCRVDLFDVAALYHEAAASGSPAIPLVRMLGELVGGDARKFVHWGATS
                     QDVIDTAVVLQIREGLDLLFDRLLETATACASLAERHRRTPRAGRTLLQHAVPITFGL
                     KAARWLSLVTRLVVSLRELRQRALVVQFGGAAGTLAALGADGVRVMEFLATEVDLAVP
                     DLPWHTERDRIAEVATRLGIVAGGMGKVATDVALLSQTEVGEAASGATSAMGGSSTMP
                     HKRNPVEATAAIACAQLAIGVVPTVLSSM"
BASE COUNT         1107 a         1888 c         1980 g         1137 t
ORIGIN      
        1 acacgcgcgc gggatatggg aagtggcggc gccctccttc accatacgat actttcatgg
       61 agcaagaggg cgtcccgatc tatcggggcg tgggggtgcg ccgcgtccaa gatcttgagc
      121 tcgcgccgtg ggggcgcatg ggcggccgtg gtactttcat tcagctgttc ggaacggaag
      181 ggttgtgggg ctgctacctt acggagatcc ccgccgccgc agcgctcgag ccggagcgcc
      241 acctttatga agaggttgtc ttggtcgtcg aagggcgcgg cgcgcttgag atctggcagc
      301 aggagggcgg gaagcgcgac ctcttagagt ggcagcccgg ttcgctgttt accgttccgc
      361 tcaacaccca tcaccggttg atcaacgcga tgtcctcggg accggcgctg ctgctagtcg
      421 gcaccacagc gccgctggtg atgaacctct tcgggaatcg cgaattcgtc ttcgactgcc
      481 ccttcgtatt ccgcgaccgc tacgatggcg gggaagaata cttcaagcca caggtgaggg
      541 aactcgaacc ggatccgcac cgcggtttgg cgatggttcg gaccaacatc gtcccggatg
      601 cgaggaatgt gcaactccca ctcgataacc gccgctcacc tgggttccga cggctggagc
      661 cgtacctcga caagaacgta ttctatcagt ggatcggtca gcatgaatcc ggacggtatt
      721 cgaaagcgca cgcgcatgaa tccgcagcgg tgctgatctg tctgggaggt aagggctaca
      781 cttacgcttg gccgcgagag gttgggacgc gcccctggga gactagtcag gcgaacaaag
      841 ttgtgcgtca ggattatgag ccggtgggaa tggtgagtgc cgcgccgatg ggcggccagt
      901 ggttccacca gcactttggt gtcgcacgcg agccgctgcg cctgatggcg tggtttggac
      961 cattcgggcg tggcacaggc cgcgagcacg ggcggcccgg cgaagaggtg ctcgacaaga
     1021 acgcccttca cttggaagag ggtggaagct cgatcgctta ttgggaggaa gatccctaca
     1081 tccgtcgcga gtacgtcggc gcattgcagc gagagggcgt cgagttccgg atggatgaat
     1141 ctttctatca gcgatccccg ggagtgaaga ccacagcgac gcgatgagca aggccgttct
     1201 tacgagtaag gatcaggccc ttcgtgaaca ggtcgtcaca gcttctcatg tgctggggct
     1261 cgaggaccag ggcgacttca tctgggggca cgtctccatt cgcgatcccg agggccgcgg
     1321 cgtgtggatg aaagccaccg gctggggatt cgaggaggtc acgccggatc gcgtgctgct
     1381 gatcggctgg gatggggaag tgctggccgg agacggccgt cgacatctgg agtatcccat
     1441 ccacactgaa atcatgcgcg cgcgttcaga tgtgaacagt gtcgttcaca cgcatgcctc
     1501 gcacgcggtc gccttcgctt ccctgatgga gccgatgcgt cccatctcac acgagggggc
     1561 catgtttgtt cctcctgaca tcgcccggtt caccaaaacc ggagatctta tcctcacgcg
     1621 cgaactgggc caaacgctcg ccgggacgct gggccagcgc aacgctgcgc tcatggtcca
     1681 ccatggcatc gtcacggccg gcgaggatgt ggagacctcg gtcgtcacgg ccattttgct
     1741 cgagcgggcc tgccagaaac aactcctcgc gatggcggcc ggtgagctgc gcacctggtc
     1801 aagtgacgaa gaagcgctgg cgaagcgctc gcgcgtgtac tcggccaagc aggcgtgggc
     1861 atatctggtc agaaagattg ggacttgatg aattcccgct acgagacagg gctgaagatt
     1921 cgcaaagagg tgctgggcgc cgatcatgtg gcccgctccc tggccgaagc cacgctggac
     1981 gccgactttc agcgctttat taccgagacg gcgtggggat cggtgtgggc acgtccggat
     2041 ctcgaccgcc gcacgcgaag cctcgtgacg atcgcgatcc tggcggccct gggccgtgag
     2101 gagctcgcgc tccaccttcg cgcgagccag aacgtcggcg tggatcagaa ggaaattgta
     2161 gaagttctcc tgcacgtggc cgcctacgcg ggcatcccgg ccgcgaacgc ggcgttcgcc
     2221 atggccaaag cgcaattcgg caataatgcc tccaaagcca aaacggggaa acgcgcccgt
     2281 ggaggacggc acggtggtta ggacctactc tccagatttc ccaccgtact tctatgaggc
     2341 atatcgctcg acggtgcggc gatcgccgcg ccagccgctc atcgcggttc cgcacacctt
     2401 gtcggagatc accggtcccg gccccgccgt cagcggcgcc gacggtgagg acaatgacct
     2461 cacacagaac ctgggctcag agggcgaggc tgtcggggaa aggatcatcg tcaccggccg
     2521 ggtgctcgat gacgacggga atccggtgcc cgacacgctg gtggagatct ggcaggcgaa
     2581 cgccgcagga cgctaccggc accggattga tcagcaccat gcgccgctgg acccaaattt
     2641 tcttggattc ggtcactgcc gcacagataa gcaaggtgtg tacagcttca ccaccgtcaa
     2701 gccaggcgcg tatccgtggg aaaatcatcc gaacgcgtgg cggcccgcgc acatccactt
     2761 ctcgctgttt ggtccttcct ggatcagccg cctcgtgacg cagatgtact ttccaggcga
     2821 tccactgcac gagttggatc cgatcttcaa ctctgtgccg acggacgcgg cgcgggacag
     2881 gctgatcgcg aactacgcgc acgacatcac aaagcccgct tgggcgctgg gctatcggtt
     2941 cgacatcatc gtgcgcggtc cggaagccac acccttcgaa ttgccgaaac ccgcccgcac
     3001 agcgaagcga tgacccgacg cggtctgaca cctccgcaga ccgtcgggcc cttcttccac
     3061 ggtggcatgc tccaccgtcc gctgaacgtg atgatcgcgc ccgagacgaa gggcgagagg
     3121 atccgcatcg aagggcacgt gtacgatggc gatcgcgccc ctgtctcgga cgcggtcgtg
     3181 gagttctggc aggccaatgc agccggtcga taccgccacc ccgccgatca gcggtccatc
     3241 cctcttgatc agtcattcgt cggctttggg cgaaccggca cggatgaagc gggaaccttc
     3301 tggttcgaaa cgatcaaacc cggagccgtg ccatttcccg gaggcggcac acaggccccg
     3361 cacattaacg tcgcagtctt ggcccggggc ctgctccatc atctaccgac acggctgtac
     3421 tttgaggatg acccgacggt tcgcgacgac ccggtgctgc gcacgattcc tgaaaagcgc
     3481 cgggccacac tgctggcgaa acggatcccg ggtgggacgc ccgcgtacca gttcgacatc
     3541 atcctgcagg gcgacggcga aactgttttc ttcctcatat aagctccgcc tatgagcaag
     3601 gccgtgcggt tcctctcgct cgccgacgcg atggtagagt acgtccaaga cggcgatgtg
     3661 gtcgccatgg agggattcac ccacctcatc cccttcgcgg ccggccacga agtgatccgt
     3721 cagggcaaac gcagcctcac gctaatccgg atgacgcccg acctgattta cgatcaactg
     3781 attggcatgg gatgcgcgcg caagctgatt ttctcttggg gcgggaaccc tggcgtgggc
     3841 tcgctgcacc ggctgcgcga tgcggttgaa aatagctggc cgaatccact cgaggtggaa
     3901 gagcacagtc acgcgggtgt atcggctgcg tatgccgccg gcgcggcggg cctgccgctg
     3961 gcggtgctgc ggggatatgt cggcaccgat cttcccagag tcaattcgaa catccgcttc
     4021 atcgaggacc ccttcacggg ggagcggctt gcggccatcc cttcgctccg tccggacgtg
     4081 tcggtgatcc atgcgcagcg cgcggatcga gccggcaacg tgctcctcga aggaatcgtc
     4141 ggtgtgcaga aagaggcggt gctggcggcg aaacgcgcgg tggtgacggt agaagagatc
     4201 gtctctgacc tgcgacctcc cagcccgaac tcggtggtgc ttcccaattg gacgattgac
     4261 gccgtcgtgg aagcgccggg aggcgcgaag ccatcgtacg cgcacggata ctacaagcga
     4321 gacaacgcat tctattccgc ctgggatgcg atctcgcgag atcggaccac gttcctcgac
     4381 tggatgcgca cgcacgtgct cgagcaaggt tccgagtctc atgcagtcca aatctgatta
     4441 caccccccct gagatgatga tcaccgcggc ggcgcgggcg ctcaagaatg atgacgtctg
     4501 ttttgtgggg attgggatgc cttctgccgc gtgcaacctg gcccgcctca cgcacgcgcc
     4561 cgacattacg ctgatctacg agtccgggac gatcgggacg aagccgcagg tgctgccgct
     4621 gtcgatcggt gatccggagc tttgcgagac tgctctgacc actgtctcgg ttcccgaggt
     4681 gtttggttac tggcttcagg ggggacgcat gacggtcgga tttctcgggg cggcgcagat
     4741 cgaccggttc ggcaacatca atacgacggt tatcggtccc tacgagcgac cgaaggtccg
     4801 tctgcccggc ggcggaggag caccggagat cgcgagttat tgccgagagg tctttgtgat
     4861 tgtctcccag acgccgcgtt cttttgtgcc gcgtgtcgac ttcattacgt cactcggatt
     4921 tggaaatggc ggcgatcaca ggcagcgtct gggtctgtcg acgctcgggc cgagcctcgt
     4981 cgtcacggac ctctgcctca tgcggcccga cccggaaacc aaagaattca tcgtcacatc
     5041 gatccacacc ggcgtgaccc gtgaggagat cacccagcgc accggttggc cggtgcgttt
     5101 ctccggcgac gtgacgcaga caccgccgcc ggagacagaa gagcttcggg tgctgcgcga
     5161 tctcattgca cgctcgccac gcgggggcgg tgcatcgtga ccgatctcgc gggcacgatg
     5221 ttcacgacgc ccgagatgtc ggcgatcttt tccggctccg cctttgtaca gcggatgctc
     5281 gacgtcgagg ccgcgctcgc ccgcgcgcac gcccgtgccg gggtcatccc gcagcaagac
     5341 gctgaagcta tcgtctccaa atgccgtgtg gatttgttcg atgttgcggc cctctaccac
     5401 gaggctgccg catccggcag tcccgcgatt ccactcgtcc gtatgctggg tgagctggtc
     5461 ggcggggatg cacggaagtt cgtgcactgg ggcgccacga gccaagatgt cattgacacc
     5521 gcggttgttc tccagatccg ggaaggcctc gatcttcttt tcgaccggct cctcgagacc
     5581 gccacggcgt gcgcctcact tgccgaacga caccgccgca ccccgagggc cggcagaact
     5641 ttgctgcagc atgcggttcc gattacgttc ggtctgaaag cggcccgatg gctgtccctt
     5701 gtgacccgcc tcgtggtgtc gctgcgcgag ctgaggcaac gggcgctcgt cgtccagttc
     5761 ggcggagccg ccgggacgct cgcggcgctg ggcgccgacg gcgtgcgtgt gatggagttc
     5821 ctggcaacgg aggtcgatct cgctgtccct gacctcccgt ggcatacgga gcgggatagg
     5881 attgcagagg tggcgacgcg actcggcatc gtggccggtg gaatgggcaa ggtcgccacc
     5941 gacgtggcgc tcctttctca aactgaagtg ggtgaagcgg cgtcgggcgc cacctccgcg
     6001 atgggcggat catcaacgat gccgcacaag cgcaaccccg tggaagccac ggccgcgatc
     6061 gcttgcgcgc agctggcgat tggtgttgtc ccgacggtgc tctcgtccat ga
//
LOCUS       DBNGSB010000185         3525 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_1055725, whole genome shotgun sequence.
ACCESSION   DBNGSB010000185 DBNGSB010000000
VERSION     DBNGSB010000185.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_1055725"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="AC159B_05360"
     CDS             <1..369
                     /locus_tag="AC159B_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3377208.1"
                     /translation="MRTTSAAAALVEVHPDVYKELFRDGPSWVRQLEEQTGRRLTIRG
                     RDGMHLERMRVLEGERTEDLDREETARDRRGQVYWLDREGEDTLVFSERDVEQELALA
                     AAGNSRNGLMRRLLKLLGRP"
     gene            652..903
                     /gene="rplU"
                     /locus_tag="AC159B_05365"
                     /pseudo
     CDS             652..903
                     /gene="rplU"
                     /locus_tag="AC159B_05365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00061.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
     gene            922..1182
                     /gene="rpmA"
                     /locus_tag="AC159B_05370"
     CDS             922..1182
                     /gene="rpmA"
                     /locus_tag="AC159B_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011318332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIR3377209.1"
                     /translation="MAHKKGMGSSRNGRDSKSKRLGIKRFAGQVVTAGSVIVRQRGTK
                     VRAGANVGLGRDFTLFALVDGVVAWERRGRDNRQVSVHPAQA"
     gene            1247..2299
                     /gene="obgE"
                     /locus_tag="AC159B_05375"
     CDS             1247..2299
                     /gene="obgE"
                     /locus_tag="AC159B_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HIR3377210.1"
                     /translation="MFIDRAVIDVKAGDGGNGSLSFRREKFVPKGGPDGGDGGHGGDV
                     VLVADESLSTLLDFQHRQHFRAQSGSHGEGSKRSGKRGGTLVVPVPVGTVVKDASSGD
                     VIADLAQHGQKVLIARGGRGGRGNARFATPTRRAPRIVEPGGPGQERRLELELKLIAD
                     AGLVGFPNAGKSTLLSRVSAARPKVADYPFTTTEPHLGVVALPDGRSFVLADIPGLIE
                     GAHQGAGLGHDFLRHISRTRVLIHLVDLSNPDRDPRADFETVNAELWQYDHALKERPM
                     LVALNKIDVPEARAKVQDVSAAVAAQGYRVFAISGATGEGVDMLMRTAAQVLDEMRNR
                     EPVLSDPGHRTPDLGS"
     gene            2309..2917
                     /gene="nadD"
                     /locus_tag="AC159B_05380"
     CDS             2309..2917
                     /gene="nadD"
                     /locus_tag="AC159B_05380"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HIR3377211.1"
                     /translation="MARVGIMGGTFDPIHYGHLVTAEEARVQFDLSQVLFVPNRYPPH
                     KDLNGVTDPEHRYRMTLLATMTNERFAVSRIEIDRPGPSFTIETIETLRKTHAASDLF
                     YITGADAILQIVHGEWERSAELLVLCQFIAASRPGFSLNAEGMRRSNVTGRRIDNLHV
                     MEIPALAISSTDIRRRVTHGRPIRYLVPDPVEAYVVKHGLYR"
     gene            2914..3255
                     /gene="rsfS"
                     /locus_tag="AC159B_05385"
     CDS             2914..3255
                     /gene="rsfS"
                     /locus_tag="AC159B_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014345466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HIR3377212.1"
                     /translation="MTSSLRLAQVAAGAMDDRKAEDVLVLDISNHTLVADYFVIGSAE
                     TNVQIKAITEGVEEAVADAGGKLLAREGHERARWVLLDFGAVVVHVFGPEARNLYRLE
                     RLWADAPIVAR"
BASE COUNT          724 a         1014 c         1165 g          622 t
ORIGIN      
        1 atgcggacga cctccgctgc ggcagcgctg gtcgaggtac atccggacgt gtacaaggaa
       61 ctgttccgcg acgggccatc gtgggtgcgc cagcttgagg agcagaccgg acggcggctg
      121 accatccgcg gccgggatgg gatgcacttg gaacggatgc gcgtgctcga aggcgagcgg
      181 actgaggatc tggaccggga agaaacggcg cgtgaccggc gtgggcaagt gtactggctg
      241 gatcgtgaag gagaggacac acttgtcttc agtgagcggg atgtcgagca ggaactggcg
      301 cttgcggctg cgggaaactc tcgcaatgga ctgatgaggc ggctgctcaa actgctgggt
      361 cgtccgtgag caaggctatg ggcctgctct cggggagggc ggagcctttc cccgactgaa
      421 actgatacgg ggaccccttt ggctccgccc tcccctacgc aggcccgata gataggatca
      481 ggctgcgacc cccggcgcag gccattgagg cgtgtgtatc tagtgtgata aaatacgcaa
      541 agtcacgagc gccgcgcaat ttgcgcgtcg agtgttatgg aggatgggat gtacgcggtt
      601 agcgagacgg gcggaataca gtacaaagtc gaatagggcg gcacgtttca tgtcgagcgg
      661 ctcgacgcag agccgggcaa ggacctcacg ctcgaccgcg tcgtcctgtt tgcagacggc
      721 aagacagtga aggtcggctc gcccgtggtc aagggtgcga aagtgcaggc aaaagtcatt
      781 gcccacggcc gcgggaagaa gctgactgtg atgaagtaca gggcaaagtc gcgctatcgc
      841 cggaagacgg gacatcgcca gcactacacg acgatcaaga ttgaagatat tctattgaag
      901 tagggcaggg gggtatccga aatggctcac aagaaaggca tgggctcgtc ccgcaacggg
      961 cgggatagca agagcaagcg gctgggcatc aagcgcttcg cgggacaggt tgtcacggcc
     1021 ggaagcgtga ttgtccggca gcgcggcacc aaggtgcggg cgggggcgaa tgtcggtctc
     1081 ggtcgggact tcacactgtt cgcactcgtc gacggtgtgg ttgcctggga gcggcggggc
     1141 cgcgacaatc gccaggtgag cgtgcatccg gcgcaagcat aagattcaaa gacgatttcg
     1201 gcaggggctt ggtcgtccgt gaccagcccc tgctgctttc catcgtatgt tcatcgatag
     1261 agcagtcatc gacgtcaaag cgggcgacgg cgggaatgga tccctaagtt tccgccgaga
     1321 gaaattcgtg cccaaaggcg gtccggatgg tggggacggc ggtcacggtg gcgacgtcgt
     1381 tcttgtggcg gacgagagcc tctctaccct gctggacttc cagcaccgcc agcattttcg
     1441 cgcgcagagc gggagtcacg gagaaggcag taagcgatcg gggaagcgcg ggggcacgct
     1501 cgtcgtgcct gttccggtcg gtaccgtcgt gaaagacgcc tccagtggag acgtcatcgc
     1561 cgatcttgca cagcacggac aaaaggtcct cattgccagg ggtggcagag gcggccgggg
     1621 aaacgcccgg ttcgcgacac caacccgccg ggccccccgg atcgtggagc ccggagggcc
     1681 aggccaggaa cgacggctcg aactggaact caaattgatc gcggacgccg gactggtcgg
     1741 ttttccaaac gccggaaagt cgacactcct cagtcgtgtg tccgcggccc ggccgaaagt
     1801 ggcggattat ccgttcacga caaccgaacc ccatcttgga gtcgtcgcac tgcccgacgg
     1861 acgcagtttc gtcttggccg atatcccagg tctcatcgag ggcgcacatc aaggcgccgg
     1921 gctgggccac gacttcctgc ggcacatctc acgcacccga gttctcatcc atcttgtgga
     1981 tctgtcgaac cctgatcgcg acccacgcgc ggactttgaa accgtcaatg ctgagctctg
     2041 gcagtacgac cacgcgctca aagagcgtcc gatgctggtg gcgctcaaca agatcgatgt
     2101 tccggaagcg cgtgcaaagg ttcaggacgt ctcagcagca gtggccgcgc aaggctatcg
     2161 cgtcttcgcg atttccgggg caacgggcga gggcgtggac atgctgatgc gaacggcggc
     2221 acaggtcctc gatgagatgc gcaacagaga accagttttg tcggaccccg gacatcggac
     2281 tccggatctc ggaagttagg tgacacgcgt ggcgcgagtc ggcatcatgg gcgggacatt
     2341 tgatcccatc cactacggcc atctcgtgac cgcggaagaa gcgagagttc agttcgacct
     2401 ctcacaggtc ctgttcgtgc caaaccgcta cccaccgcac aaggatctca acggagttac
     2461 agatcccgaa caccgctaca ggatgacatt gctggcgacg atgacgaacg agcgcttcgc
     2521 cgtgtcgagg atagagatcg accgcccggg cccatcgttc accatcgaga cgatagagac
     2581 tctccgcaag acccacgcgg cgtcggatct cttttacatc acaggcgcgg acgcaatctt
     2641 gcaaatcgtg cacggagaat gggaacgatc ggcagagctg ctggttctct gccagttcat
     2701 cgcggcgagc cgcccgggct tctcgctaaa cgctgaaggc atgcgtcgca gcaatgtgac
     2761 cggccggcgg atcgacaacc tccacgtcat ggagatcccc gcgctggcga tctcgtcgac
     2821 cgacatccgg cggcgcgtca cgcatggccg tcccattcga taccttgttc cagatccggt
     2881 tgaggcgtac gtcgtcaagc atgggctcta ccgatgacgt catcgctccg cctcgctcag
     2941 gtggccgccg gcgcgatgga tgatagaaag gccgaggacg tgcttgtgct cgacatcagc
     3001 aaccacaccc tcgtcgccga ctacttcgtg atcggctccg ctgagacgaa cgtacagatc
     3061 aaggcgatta ccgagggcgt cgaagaggcc gtggctgatg cgggaggcaa attgctggcg
     3121 cgtgaggggc acgagcgggc gcgctgggtg ttgctggact ttggagcggt tgtggtgcac
     3181 gtgtttgggc ccgaagcgag gaacctctac cgcctggagc gcctctgggc cgatgcaccc
     3241 atcgtcgcgc gctgacttcg cttggcgctc cccaaccatc ctcgcttgac gcgtattcgc
     3301 cgcgctgcct ataatgttcc aagtctcata gaggcgatga tcggaagtag taggtttggt
     3361 gaaggcgtag cgagccggag gaacgggtga aatgaccggc gcctgaacca gccgaactcg
     3421 tccgggagct gccgacccga aaacagcggt cataggtcgt aggtcattgg tcgtggacag
     3481 atcaatcgta attgccacga cctacgagcc acgaccaacg cctgc
//
LOCUS       DBNGSB010000186         3881 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2897673, whole genome shotgun sequence.
ACCESSION   DBNGSB010000186 DBNGSB010000000
VERSION     DBNGSB010000186.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2897673"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(283..1122)
                     /locus_tag="AC159B_05390"
     CDS             complement(283..1122)
                     /locus_tag="AC159B_05390"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DF family (seleno)protein"
                     /protein_id="HIR3377213.1"
                     /translation="MKIEFLYWEECPSHGEALARLRDVLKEAGVGVPVELIHVGTDDQ
                     AVSMKFPGSPTIRVDGADLFPISDEVVGLTCRVYHTPEGRVSPLPTKEMIHVALKWRL
                     EMVLALESKLLTFSLPGTDGKTHSPDQYRQARALGVVFTCNHCPYAKAWEDRLIQLQR
                     DFAKSGVQLLLVSSNDPAKIPDDSFENMKRRAEEKAYPFPYLFDESQEVARSYGATRT
                     PEIFLFDADRVLRYHGAPDDNFEDPAAVTQHYVRDAIDALIAGRRPEPRETKPVGCTI
                     KWK"
     gene            1168..1542
                     /locus_tag="AC159B_05395"
     CDS             1168..1542
                     /locus_tag="AC159B_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HIR3377214.1"
                     /translation="MRLSRKKFEVLVLDALESIPEPVRERMQNIDVVIEDWPEQDQLT
                     ELGMEPDEWLFGLYEGTPLTERGISEHPLLPDKITIFQGPLEDAHESDHEIKNEIRKT
                     VVHEVAHHFGITEERLAELGYD"
     gene            1597..2370
                     /locus_tag="AC159B_05400"
     CDS             1597..2370
                     /locus_tag="AC159B_05400"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3377215.1"
                     /translation="MRLRILRSGSAGNAILVDAGTARLLIDAGLPAEVIVRELGALSV
                     LPQDLTAVLLTHEHDDHAKGALTLSRIAGCPVFANEGTIASAGAALDGAQIERFATGI
                     PFRVGALTIDAFALPHDAAEPVGFAISQNGSRLVIATDLGSSNDEVVERARDADMLVI
                     ESNYDLRLLSVSPYPWFLKNRILSTTGHLSNDAAAETIRDAAAGRLRTAVLVHLSDIN
                     NLTPLARDTVQWTLEREAITHVAVHAVRPNGTSKTYELQ"
     gene            2392..3750
                     /locus_tag="AC159B_05405"
     CDS             2392..3750
                     /locus_tag="AC159B_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase type-2 family protein"
                     /protein_id="HIR3377216.1"
                     /translation="MIFRDNLFRGKPNEELRRQAAAIFDAAVRAADPAAAVRRHVSLD
                     RSTLRIDGRSYDLERLDRIFLIGAGKASGPMAKTLEDLLGERITAGIVNVKYGYTVPL
                     ERVSLVEAGHPIPDDAGFRGAERILDLVEQAGPHDLVLCVISGGGSALLPAPVDGVSL
                     AEKARVTDLLLKSGATIQEINAVRKHLSKIKGGQLARAAAPAEVAVLILSDVVGNPID
                     AIASGPAVPDPTTFADAREVLKRYGLTAEAPGGLVDYLNRGAAGLVPETPKPGNPIFE
                     RVQTVIVGSNDVAVQAAARSAQASGLQTVVLPTVTGEARDAAAMFSDRALQFANDIRP
                     ACVLAGGETTVRVRGSGMGGRCQEFALAAALKIAGRQRTLVAAFGTDGTDGPTDAAGA
                     VADGTTLGRADAAGLDARRALDDNDAYTFFSLLSDLIVTGPTNTNVNDIYLALMSPRE
                     GA"
     gene            3747..>3881
                     /locus_tag="AC159B_05410"
     CDS             3747..>3881
                     /locus_tag="AC159B_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HIR3377217.1"
                     /translation="MKVLLCVSEAVPFAKTGGLADVAGALPLALAELGCEVRLALPRY
                     R"
BASE COUNT          737 a         1187 c         1233 g          724 t
ORIGIN      
        1 gcctccatct ccacttcgcc gatcccggcc gccgtctcac cggctagatc cgggtcagca
       61 ggagacgcaa acgcggccgt agacagcaag agcgagaaga gggctacgag cgttaccagc
      121 ttaccagtca tggtagttac ctcctttctc tgcaatttgt tgtgaggcaa gcatacgata
      181 ggggccgttc gacggcagtg acggacctaa tagggcaggt gcgatgtgcg tcacacctgc
      241 ccgttaacga gcgcagaacg acggaggagc tttacgcggc ggtcacttcc atttgatcgt
      301 gcacccgacg ggtttggttt ccctgggttc ggggcggcgt ccggcgatca gcgcgtcgat
      361 cgcgtcccgc acatagtgct gcgtgacggc ggccgggtcc tcaaagttgt cgtcgggcgc
      421 gccgtgatag cgcagcacgc ggtctgcatc aaacaggaag atctccggag tacgtgtcgc
      481 gccatacgac ctcgcgactt cctgcgattc gtcgaacaga taggggaagg gataggcctt
      541 ctcctccgcc cgccgtttca tgttctcgaa actgtcgtcg ggaatcttcg cgggatcgtt
      601 cgagctgacc aacagcaact gcaccccgga tttcgcgaag tcacgctgca gctgaatcaa
      661 ccggtcctcc caagccttgg cgtaggggca gtgattgcag gtgaatacca cgccgagggc
      721 tcgtgcctgc cggtactgat ccggactatg cgtctttccg tctgtccccg gcaatgagaa
      781 cgtgagcaat ttcgattcga gtgcgagaac catctccagc ctccatttga gagctacgtg
      841 aatcatttcc ttcgtcggaa gcggcgagac gcggccttca ggcgtgtggt agacacggca
      901 ggtcaatcct accacctcgt cggagatcgg aaacaaatct gcgccgtcaa cgcggatcgt
      961 gggcgagccc ggaaacttca ttgagaccgc ctggtcgtcc gtcccgacgt gaatcagctc
     1021 gacgggaacg ccgacccctg cctccttcag cacgtcacgc agccgggcaa gggcttcgcc
     1081 gtgagacggg cattcttccc aatacaggaa ctctatcttc acgattctct ttctctataa
     1141 tacgttgtct gtcgtactat atttccgatg cgactgtctc gcaagaagtt tgaggtgctg
     1201 gtcctcgacg cgctcgagag catcccggag ccggtccggg agcgcatgca gaatattgac
     1261 gtggtgatcg aagactggcc ggagcaagac cagctcacgg agctcggcat ggagccggac
     1321 gagtggctct tcgggctgta cgaggggacg ccgctcacgg agcgggggat ctcggaacac
     1381 cccctcctgc cggacaagat caccatcttc cagggtccgc tggaagacgc ccatgagtca
     1441 gaccatgaaa tcaagaacga aattcgtaag accgtcgtgc acgaagtcgc gcaccatttc
     1501 ggcattactg aagaacggct ggcagaactt ggctacgatt agaacaggac ttgaccccgc
     1561 cgagccgtaa ccagtaggcg ggactctaca acgaccttgc gactacgaat ccttcgaagc
     1621 ggaagcgccg gaaacgccat cctcgtggac gcgggcaccg cgcggttgtt gatcgacgcg
     1681 ggactgcccg cggaagtcat cgtccgagaa ctgggggcgc tctccgttct tccccaggat
     1741 cttacagccg tgctgctgac ccatgaacat gatgaccatg caaagggcgc gctgacgctg
     1801 agccggattg ccggatgccc ggtcttcgcg aatgagggga cgattgcatc cgccggcgcg
     1861 gcgctcgacg gagcccagat cgagcgtttc gcgaccggga ttccgtttcg agtgggtgcg
     1921 ctgaccatcg atgcattcgc cctcccccac gatgccgccg aaccggtcgg ctttgccatc
     1981 agtcagaacg gctcgcgact tgtcatcgcc acggaccttg gttcctccaa tgacgaggtg
     2041 gtcgagcggg cgcgggacgc ggacatgctt gttatcgagt cgaactacga cctgagactc
     2101 ctatccgtca gtccgtatcc ctggtttctc aagaaccgca ttctgagcac cacggggcat
     2161 ctgtccaacg atgccgcggc ggaaacgatc cgggatgcgg ccgccgggcg tctgcggacg
     2221 gcagtgctcg tccatttgag cgacatcaac aacctgaccc cactggcgcg cgataccgtg
     2281 cagtggacgc tcgagcgtga ggccatcaca cacgtcgcgg tgcatgccgt gcgtcccaac
     2341 gggacgagca aaacctacga actgcagtga gctgttcagc atagttccct gatgattttc
     2401 agggacaacc tgtttcgtgg gaagcccaat gaagaattgc gccgccaggc cgcagccatc
     2461 ttcgacgcgg ccgtgcgcgc ggcggatcct gctgccgcgg tccggcgcca tgtgtcactg
     2521 gatcgcagca cgctgcgtat cgacggccgg tcgtacgatc tcgagcgact cgaccggatt
     2581 ttcctgatcg gcgccgggaa ggcgtccggc ccgatggcga agacgctcga ggatctgctc
     2641 ggcgagcgga tcaccgccgg aatcgtcaac gtcaagtatg gatacacggt cccgctcgag
     2701 cgagtctcac tggtcgaagc cggccatccg attccggatg acgcgggttt ccgtggcgcg
     2761 gagcggatcc tcgacctcgt ggagcaggcc ggtccccacg atctcgtttt gtgcgtcatc
     2821 tcggggggag gatcggcact gctgcctgcg cctgtcgacg gcgtttcgct cgcagagaaa
     2881 gcccgggtca ccgatctgct gctcaagagc ggcgcgacga ttcaggaaat caacgcggtg
     2941 cgcaagcacc tctccaagat caaaggaggg cagctggcca gagcggcggc cccggccgag
     3001 gtggccgtcc tgatcctttc ggatgtcgtg ggcaatccca tcgacgcgat cgcgtcgggc
     3061 ccggctgtgc ctgatcccac cacgttcgca gacgcccgag aggtcctgaa gcgatacgga
     3121 ctgaccgccg aagcaccggg tggcctcgtc gactacctga accggggtgc agcaggattg
     3181 gtaccggaga cgcccaagcc ggggaacccg atctttgagc gggtgcagac cgtgatcgta
     3241 ggcagcaatg acgtcgcggt ccaggccgcg gcccgctcgg cccaggcgtc gggactacag
     3301 acggttgtgt tgccgacggt gaccggcgaa gcgcgagatg ccgctgcaat gttctcggat
     3361 cgggcgctgc aattcgcgaa tgacatccgg ccggcatgcg tgctggcggg aggcgaaacc
     3421 acggtcaggg tgcgggggag cggaatgggc ggtcgttgtc aggagtttgc gctggctgcg
     3481 gcgctgaaga ttgccgggcg gcagcggacg ctcgttgccg cattcggcac agatggcacc
     3541 gatggtccca cagacgcggc cggagccgta gcggatggca cgaccttggg ccgggccgat
     3601 gccgccggtc tcgatgcgcg gcgggcgctt gacgacaatg atgcgtacac gttcttctct
     3661 ttgctgtcgg acctgatcgt gacggggccg acaaacacaa acgtcaatga catctacctg
     3721 gcactgatga gcccaagaga gggtgcatga aagtcctcct gtgtgtttcg gaagccgtgc
     3781 cgtttgccaa gaccggcggt cttgcggacg ttgcgggcgc actccccctg gcacttgcag
     3841 agctgggatg cgaggttcgc ctcgcgctcc ctcgatatcg c
//
LOCUS       DBNGSB010000187         3669 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3204143, whole genome shotgun sequence.
ACCESSION   DBNGSB010000187 DBNGSB010000000
VERSION     DBNGSB010000187.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3204143"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="AC159B_05415"
     CDS             complement(<1..455)
                     /locus_tag="AC159B_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007752025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3377218.1"
                     /translation="MKRFAFVLSRLLQTMLVLLVVATMLFLIFRLMPGNPLVAYLDPT
                     FTEDMERQLRAQFGLDKPLHVQYALFLRNAVRGEFGNSFFTREPVNEVIWTVLPNTLL
                     LTLSALLLAYVVGIAGGALLAARRGTWVEHLGIPAVLATRAAPEFWVGML"
     gene            complement(505..2169)
                     /locus_tag="AC159B_05420"
     CDS             complement(505..2169)
                     /locus_tag="AC159B_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007752023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3377219.1"
                     /translation="MARGWLRIVVVTLALALAFGTVASGQSQDRRVRDLILLTRPQAV
                     DPREFETARFLAPEFEKLGVKVQVRVMPWEQMADYVWYSRDKWDMTMWQMVGRPERSD
                     PDEFAMNLFHASTADKGYNFVGYLNRTYDGIAEAQRIKTDLKSRQLLIREAQKIIARD
                     APYIFLVFPESAYAYNNTVWDTKTVVEQKGIGIKNFWTFVGARPLGSQRDMVLNANDI
                     VQAINPLFISGAADSWVTELIWDRLFRVGADGLPRPWAAEKADWVDSVTLDVTLRRNM
                     KFHDGRPVTADDVIFSFTAAAGGEVPMYRPFVSNLERIEKRSDTVIRFVLKDPYAPFL
                     ISSLAKLNIIPKHIWEPVLADLARKPENAESYQEQVPVGSGPFKFVQWKKSEEIVLEA
                     VSDHFAAPKMRRWVLRIVPNVEAALGGLRTGEINFLANYSGDPQILAKMAQDNRSITL
                     VSTMDVGMRFLAFNLRRPPFDNAAFRQALNMATNKRAIRSVVYKNFAAIADSFVSPAL
                     EFWYNPEIPKWEYNIGAAREHLAKAGFQWDRDGRLLYPQGKIETLK"
     gene            complement(2222..2959)
                     /locus_tag="AC159B_05425"
     CDS             complement(2222..2959)
                     /locus_tag="AC159B_05425"
                     /inference="COORDINATES: protein motif:HMM:NF013353.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047661 - amino-acid racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HIR3377220.1"
                     /translation="MRLLYILPGSLSRTELGVREIERRRGILQRMAAPGTQVGITDVP
                     DGPSSIESAYEEYLSVPGTLQRVQAAERDGYDAAIVGCFGDPGVDAARELVEMPVVGP
                     AEASMLFACTLGHSFAVITVLESVVAPLRHLAHRIGVAPKLASVRGVDIPVLDLGKDR
                     ARSVGRMIALGRRAVEEDGADALVLGCMSMGFLEAHEEIAAETGVPVVNPVYAALKMA
                     EALHGAGLRHSKRAYPLPPKLRTVHVG"
     gene            complement(3048..>3669)
                     /locus_tag="AC159B_05430"
     CDS             complement(3048..>3669)
                     /locus_tag="AC159B_05430"
                     /inference="COORDINATES: protein motif:HMM:NF013696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR3377221.1"
                     /translation="VKPPKPEDERLLRDLAESFDPELVLRENDVRRFRYDVAGADLLR
                     RYLFEPILNIDGIAGGHYGAGSKTLLPHEVRAKMHFRLVPDMEPQEVRAKFLAHLKKI
                     GCSDFDVHFEEGYPWAVMSPSAPISQAMIRAMRAFDLEPQVWPNIGGSAPFYLFSRVL
                     KQPFTMGGLGHGGRVHSPNEFALVDGIRMYEKSVVQFLHEFAGIRE"
BASE COUNT          681 a         1200 c         1143 g          645 t
ORIGIN      
        1 agcatgccca cccagaactc cggcgccgcg cgggtagcca ggaccgcggg gatgcccaag
       61 tgctccaccc acgtgccgcg gcgcgccgcg agcagcgctc cgcccgcaat tcccacgaca
      121 taggccagca gcagggcgct aagcgtcagg agcagcgtgt tgggcagcac ggtccagatg
      181 acttcattca cgggttcgcg cgtgaagaac gagttgccga actcgccgcg gacggcgttg
      241 cggagaaaaa gcgcgtactg cacatgcagc ggcttgtcca ggccgaactg cgcgcgcagt
      301 tgacgctcca tgtcctcggt gaaagtcgga tcgaggtagg caaccagcgg gttgcctggc
      361 atcagccgga agatcagaaa gagcatcgtg gccacgacca gcagcacgag catcgtctgg
      421 agaaggcgac tgaggacgaa cgcgaagcgc ttcacgggcg ggggacgggg cggagaccgc
      481 ttggtccccg ccccgcggaa aacgctactt cagtgtctcg atcttgccct gtgggtacag
      541 cagccggccg tcgcggtccc actgaaaacc tgccttcgcc aggtgctcgc gcgccgcgcc
      601 gatgttgtac tcccacttgg ggatctcagg attgtaccaa aactccagcg cgggcgagac
      661 gaacgagtcg gcgatggccg cgaagttctt gtagacgacg gaccggatgg cccgcttgtt
      721 ggtagccatg ttcaaggcct gccggaacgc ggcgttgtcg aacggcggac ggcgcaggtt
      781 gaacgcgagg aagcgcatgc ccacgtccat cgtagagacc agcgtgatgg agcggttatc
      841 ctgggccatc ttcgcgagga tctggggatc gccgctgtag ttggcgagga agttgatctc
      901 cccggtgcgc agaccgccga gggccgcttc gacgttgggg acgatgcgca gcacccaccg
      961 ccgcatcttg ggcgcggcga agtggtcgga gacggcctcg agaacgatct cctcggattt
     1021 cttccattgc acgaacttga acggaccgga gccgaccggc acctgctcct ggtaagactc
     1081 cgcattctca ggcttcctgg caaggtcggc aagcacgggc tcccaaatgt gctttgggat
     1141 gatgttcagc ttggccaggc tggagatcag gaacggcgcg tacggatctt tcagcacgaa
     1201 ccggatcacc gtgtccgagc gcttctcgat gcgctcgagg ttgctgacga agggccggta
     1261 catcggcacc tcgccgcccg cggccgcggt gaacgaaaag atgacgtcgt ccgcggtgac
     1321 ggggcgaccg tcgtggaatt tcatgttgcg gcgcagcgtc acgtcgagcg tgaccgagtc
     1381 cacccagtcc gccttctcgg ccgcccaggg ccgcggcaga ccgtccgcgc cgacgcggaa
     1441 gaggcggtcc cagatcaact ctgtgaccca tgaatccgcc gcgccgctga tgaagagcgg
     1501 gttgatggct tgcacgatat cgttggcgtt gaggaccatg tcccgctggc tgccgagcgg
     1561 acgcgccccc acgaaagtcc agaagttctt aatgccgatg cctttctgct cgacgaccgt
     1621 cttcgtatcc cacaccgtgt tgttgtacgc gtacgcgctc tcggggaaga caaggaaaat
     1681 gtagggcgcg tcccgggcga tgatcttctg tgcctcgcgg atgagcagct gccgcgattt
     1741 gagatcggtc ttgatgcgct gggcttcagc gataccgtca taagtcctgt tcaggtagcc
     1801 gacgaagtta tatcctttgt cggcggtgga tgcgtggaac aggttcatcg cgaactcgtc
     1861 ggggtctgag cgctcgggcc gtccgaccat ctgccacatc gtcatgtccc atttgtcccg
     1921 gctgtaccag acatagtcgg ccatctgctc ccagggcatc acccgcactt gtaccttgac
     1981 gccgagcttc tcgaactcgg gcgccaggaa tcgcgcggtc tcaaactccc gggggtcaac
     2041 ggcctgcggc ctggtcaaca gaatcagatc ccgcacgcgg cggtcctggc tttggcctga
     2101 cgccacggta ccaaatgcca gcgccagcgc gagagtgacc accacgatgc gcaaccagcc
     2161 tcttgccata aatgccatcc ccctttcgaa tcgggcgtcg ccgcccgtgt cctgccttgc
     2221 gttatccgac gtgtacggtc cgcagcttcg gcggcaacgg gtaggcgcgt ttgctgtgcc
     2281 ggaggcctgc cccgtgcagc gcctcggcca tcttgagcgc ggcgtacaca ggattgacca
     2341 ccggtacgcc ggtttcggcg gcgatctcct catgggcctc gaggaatccc atgctcatgc
     2401 atccgaggac caaggcgtcc gccccgtcct cttccaccgc tctccgaccg agcgcgatca
     2461 tgcgacccac gctgcgcgcc cggtccttac cgagatccag gaccgggatg tccacgcccc
     2521 ggacggaagc gagcttcggg gcgactccaa tcctgtgcgc gagatggcgc aacggagcga
     2581 cgacggactc cagaacggta atcacggcga agctgtgacc gagcgtgcag gcaaaaagca
     2641 ttgacgcctc agccgggccc accacgggca tctcgaccag ctcgcgcgcg gcatccactc
     2701 ccggatcacc aaagcacccg acaatggccg cgtcgtatcc gtcacgctcg gcggcctgaa
     2761 cccgctgcag cgtgccgggc acagacaggt actcttcgta tgccgactcg atcgagctgg
     2821 gaccgtccgg aacgtcggtg atcccgacct gcgtgcccgg cgccgccatg cgctgcagga
     2881 taccgcggcg gcgttcaatc tctcgcacgc ccagctcggt gcgggaaagg ctgcctggca
     2941 agatgtatag cagccgcatc gagtctacct cctcacggat acgtaccagc aagggtagtg
     3001 ggccattgcc ttcgtagagc tggctccggt atccttccgc cgcacgctta ttccctgatc
     3061 ccggcgaact cgtgcagaaa ctgcaccacc gacttctcat acatccggat cccgtcaacg
     3121 agcgcaaact cattgggact gtgcacgcgc ccgccgtgac ccagaccgcc catggtgaac
     3181 ggctgcttca gcacccggct gaacaggtag aatggcgccg agccgccgat gttcggccag
     3241 acctggggct cgagatcaaa cgcgcgcatg gcgcggatca tcgcctgcga gattggggcg
     3301 gacgggctca tcaccgccca gggatacccc tcctcgaagt gcacgtcgaa atcgctgcag
     3361 ccgatcttct tcagatgcgc caggaatttc gcccgcactt cctgaggctc catgtccggc
     3421 acaaggcgaa aatgcatctt cgcccgtacc tcatggggta gcagcgtttt cgagccggcg
     3481 ccgtaatgac cgccggcgat gccgtcgata tttagaatgg gctcgaacag atagcggcgc
     3541 aggaggtcgg cgcctgccac atcatacctg aagcggcgca catcattctc tcgcagcacc
     3601 agctccgggt cgaatgactc ggccagatcc ctcagcagcc tctcatcttc cggcttgggc
     3661 ggcttcacg
//
LOCUS       DBNGSB010000188         5306 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3204158, whole genome shotgun sequence.
ACCESSION   DBNGSB010000188 DBNGSB010000000
VERSION     DBNGSB010000188.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5306)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5306
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3204158"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..1596)
                     /locus_tag="AC159B_05435"
     CDS             complement(214..1596)
                     /locus_tag="AC159B_05435"
                     /inference="COORDINATES: protein motif:HMM:NF024825.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3377222.1"
                     /translation="MTQQQQLNRRNFLNSLGITVGAAAATSVPAVAPAVAQVARPPKG
                     NIPSTPLKFGHMTFLTGPAAVLGDPSLKGHILAAEEINAQGGILGKRKIETITADENA
                     GTDANVKELRRMKLSEKIDMFTGVISSGNTPALGPVAEELGLLTIFVDGCTDFLWDKA
                     VPNPKFAFRITNIQSADGVTCAVAAAQTWPTAKKIAHIHPDYSYGRNAFDHFMIAYKK
                     MVPGAEVVSEGWPKLGTTDFTAHITKTMSSNPDLIVSSVWGGDYVALYKQALRYGMFN
                     NAKFASTIAFGVAPHAIGKDHPEGVIAGVHSNYYFTFPPGNRWPANKTFVEKYFKRWN
                     EYPNFQSDGAYHTLHMYRTAVEKANRLVGGWPDEEAIISQLEGMYFETAAGYLYIRPD
                     NHQGYKDAVTGFSKNVAEYPFQILDPGRMITIPIRNITAPPNWPKPGTSHNDPTSTYN
                     WIKETWPQVV"
     gene            complement(1933..2904)
                     /gene="hflK"
                     /locus_tag="AC159B_05440"
     CDS             complement(1933..2904)
                     /gene="hflK"
                     /locus_tag="AC159B_05440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HIR3377223.1"
                     /translation="MTASHEDVVPQDRAESAPRHPRWRAHALRGGVVFVLLLIAAYFA
                     TGFHLVATDEQAFARRLGAPHARLGPGMHWRFPWPVDRVDVLKTTSVSKVGVGFALPE
                     GEQETVIGMELLTGDTNILSIALVLQYVIRDPADFLLHVEDTRALIGGLAESVLTQVL
                     AGMSVDEVLTTGRLAIQDRVKIQTQELLDRYRSGVQITASSIMAMTLDAAVAQAFQDV
                     ADAMADRERSRNEARVYAANLLPKARGEAHTLVQAAQSRREQRIAQALGTTTRLKALL
                     QEYAKAPELTRTRLYLEAMEKTLPRVKAYILGTEDGQAPVNLRVRGR"
     gene            complement(2901..3905)
                     /gene="hflC"
                     /locus_tag="AC159B_05445"
     CDS             complement(2901..3905)
                     /gene="hflC"
                     /locus_tag="AC159B_05445"
                     /inference="COORDINATES: protein motif:HMM:NF013324.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0098797 - plasma membrane protein complex
                     [Evidence IEA];
                     GO_process: GO:0052547 - regulation of peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HIR3377224.1"
                     /translation="MSRAGGRRRGALRVLTPAAIAVLVLSALASLFTLDITEHGMVTR
                     FGRIVQLVEEPGLGFKLPFERVQRFDKRLLYLSTRPVEYVTADRKNVSVATLATWRIA
                     EPTRFLATLATRGDAEQRLTEAIVGEVGAVLGRHSFASLIAPHASENRFGAIVAEVLA
                     GVQALVAPAYGIEVIDLGVRELALPQQNRQSVFERMKAERARITMQLRSDGKAESRRI
                     IAEADRQKARILAEANEHARRLDGEGEAEAMRVYAREFGADLPFFKLVRTLETYDRIL
                     DDKTTLFLPAESELLQMLQPSASVARDLNPPPKARPRNGETVGAGVEGQQPPSRGESP
                     "
     gene            complement(3931..>5306)
                     /gene="hflK"
                     /locus_tag="AC159B_05450"
     CDS             complement(3931..>5306)
                     /gene="hflK"
                     /locus_tag="AC159B_05450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HIR3377225.1"
                     /translation="ALAANVASVVILVSYKDGDANVRSVWLCSRNDAIGNVAVMLAAL
                     GVWATATKWPDLIVAAIMAGLFLWSSAQILRQAVRELRTGEISLHTHAPHTHAPPEWV
                     RLRDRFAAATRVFVTDTRVKIAALASLVLLWLASGFFTIQSGEIGLRLRFARIVAADL
                     GPGLHYRLPWPIETHRLVETERTRSAELGFRSQADESLTTRTLARERLTVGGPSNPVP
                     EAIKAKGSWFQREVLEGESFLLTGDANLIDVRSTVQYRVNDPVAYVTNLAEPDSLVRS
                     VTRAALRSVVATQRIDALLTNARAEVEARLHEFVQARLDGLNAGIALVSVRLVYVHSP
                     QEAHDAERDVASAQEDKLRTINRAKGFAAEEVNEAKGEAAATVEGALAVQEEQVRRAR
                     GEALAFSLQVEAFRQAPELAAFRLQMETIEDVLPKVHKLVRPKASAIKDFDLWLLEPP
                     ISAGGNR"
BASE COUNT          921 a         1686 c         1703 g          996 t
ORIGIN      
        1 gcggatttcc tgcgggtaga acgagggata gtacggcacc tcatgcccgg cctgcgccgc
       61 cggcgcagtg gcgcaaagca gcgtcgctgc gagcgtgccg ctttgcatga gggatctccg
      121 cacggacatc acgaccctcg aggcaacgcc ggccctgcaa caaaccgacc acgccctcgc
      181 ggaacgaagg catggttggt tgatgggcaa gcttcagacc acctgtggcc aagtctcctt
      241 gatccaattg taggtcgacg tcggatcgtt gtgcgaggtg cccggcttcg gccagttcgg
      301 cggcgccgtg atgttgcgga tcgggatcgt gatcatcctg cccggatcga ggatctggaa
      361 cgggtactcg gccacgttct tgctgaaccc ggtcaccgcg tccttgtagc cctgatgatt
      421 gtcgggccgg atgtagagat agcccgccgc ggtctcgaaa tacatgcctt cgagctggct
      481 gatgatcgcc tcttcgtccg gccagccgcc gacgagcctg ttcgctttct cgaccgccgt
      541 acggtacata tgaagcgtgt ggtacgcgcc atccgactgg aagttcggat actcgttcca
      601 gcgcttgaaa tacttctcca cgaaggtctt gttggccggc cagcgatttc ccggcgggaa
      661 ggtgaagtag tagttggagt ggacgccagc gatgacacct tccgggtgat ccttgccgat
      721 ggcgtgcggc gcgacgccga aggcgatcgt gctcgcgaac ttggcattat tgaacatgcc
      781 atagcgcagc gcttgtttgt aaagcgccac gtagtcgccg ccccacaccg acgacacgat
      841 caggtcgggg ttcgacgaca tcgtctttgt gatatgcgcg gtgaagtcgg tcgtgccgag
      901 cttcggccaa ccttccgaca cgacctcggc gccgggcacc atcttcttgt aggcgatcat
      961 gaagtggtcg aaggcgttgc ggccgtaaga gtagtcggga tggatgtggg cgatcttttt
     1021 tgcggtcggc caagtctgcg ccgccgcgac cgcgcaggtc acgccgtcgg ccgactggat
     1081 gttggtgatg cggaacgcga acttcgggtt cggcaccgcc ttgtcccaca agaagtcggt
     1141 gcagccatca acgaagatgg tgagcagacc gagctcctcg gcgaccggtc cgagcgcggg
     1201 ggtattgccg ctcgatatga cgccggtgaa catgtcgatc ttctcggaga gcttcatgcg
     1261 gcgaagctct ttgacgttgg cgtcggtgcc ggcgttctcg tccgccgtga tggtctcgat
     1321 cttgcgcttg ccgagaatgc cgccctgcgc gttgatctcc tcggcggcca ggatatggcc
     1381 cttgagcgag ggatctccga gcaccgcggc ggggccggtg aggaaggtca tgtgaccgaa
     1441 cttgagcggg gtggagggaa tatttccctt gggtggcctg gcgacctgcg ccacggccgg
     1501 agcaaccgcc ggcaccgaag ttgccgcggc ggcccctacc gtgataccaa gactattcag
     1561 gaaatttctg cggttcagct gctgctgctg cgtcatggac cgtcctccct tcgaaggtcg
     1621 ggtgcgaacg ctctcgccac gctcggagca cgcacctccg gccttttatg gcttatcgca
     1681 acatcttaag ccttcggggc ctccgagtaa atcaacttcg gccggttctg agcgcaatcg
     1741 gctgtccatc cgggtcgcga gatctcatca ggccgcgagc ccgcgtcccg acttctgccc
     1801 attccattgc atctgctcca gaccactcaa ggcgggccga tcactcagcg tcattgccta
     1861 aatgagccag cgggctggag ctcggccgaa aagacacccg gtggggatca ggtgctgttc
     1921 gctggacaga atctaccgcc ctctcactcg caagttgacc ggagcctgtc catcctccgt
     1981 gcccaggatg taggctttca ctctgggcag agtcttctcc atggcttcaa gatagaggcg
     2041 ggtgcgcgtg agctcggggg ccttcgcata ctcttgcagt aaagctttga ggcgggtggt
     2101 cgtgcctaag gcttgcgcga tgcgctgctc acgccggctc tgcgctgcct ggaccaatgt
     2161 gtgcgcctcc ccgcgagcct taggcaggag gttggccgca tagacccgcg cctcgttgcg
     2221 tgagcgctcg cgatcggcca tggcgtccgc cacgtcttga aaggcctgtg ccaccgccgc
     2281 gtcaagcgtc atggccatga tgctggaagc cgtgatctga acgccgcttc ggtagcgatc
     2341 gaggagctcc tgagtctgta tcttcaccct atcctggatg gcaagccggc ctgtggtcaa
     2401 gacctcatca accgacatgc cggccaaaac ctgggtgagc acactttcgg caagcccgcc
     2461 gatcagcgct cttgtgtcct cgacatgcag caggaagtcg gcaggatcgc gtatcacgta
     2521 ttgtagcacg agcgcgatgc ttaggatgtt cgtgtcacca gtcagcagct ccatcccaat
     2581 cacggtctcc tgctcgccct cgggcaaagc gaagcccacg ccgactttcg agacacttgt
     2641 ggtcttaagc acgtcgaccc ggtcgacggg ccaggggaaa cgccagtgca tgccggggcc
     2701 caggcgggca tggggcgcgc cgaggcggcg cgcgaaagct tgctcgtccg ttgcgacgag
     2761 atggaagccc gtggcgaagt aggccgcgat cagcaggagc acgaagacga caccgccccg
     2821 gagcgcgtga gcgcgccagc gcggatgtct gggggcgctt tccgcgcggt cctgcggcac
     2881 cacgtcttcg tggctggccg tcatggcgat tctccgcgtg acggtggctg ctgaccttcg
     2941 acaccagcgc ccacggtctc gccattcctg ggacgagcct tgggcggcgg gttaagatcc
     3001 cgcgcgaccg atgcgctagg ctgcagcatc tgcaggagtt cggactcggc cggcaggaac
     3061 agggtcgtct tgtcgtcgag gatacgatca taggtctcga gcgtgcggac gagcttgaag
     3121 aagggtagat cggcgccaaa ctcgcgcgcg tagacacgca tcgcctcagc ctccccctcg
     3181 ccgtcgagtc gccttgcatg ttcgttggcc tcggcgagga tgcgtgcctt ctgccgatca
     3241 gcttcggcga tgatccgcct cgactcggcc ttcccgtcgg agcgcagctg catggtgatg
     3301 cgggcccgct ccgctttcat ccgctcgaag acgctctgcc ggttctgctg tggcagggcg
     3361 agttcacgca caccgagatc gatcacctcg ataccgtagg cgggcgcaac aagagcctga
     3421 acgccggcaa gtacctccgc gactatcgcc ccgaaccggt tctccgaagc atgcggcgcg
     3481 atgagggagg cgaagctatg gcggccgagc actgcaccga cttcaccgac gatagcctcc
     3541 gtaagccgct gctcggcatc gccgcgcgtg gcaagcgtcg cgaggaagcg cgtcggttcg
     3601 gcgatgcgcc aggtagcgag cgtggcgacg gacacgttct tcctgtccgc cgtgacgtac
     3661 tcaacgggcc gagtgctcaa gtataggagg cgcttgtcga accgctgcac acgctcgaag
     3721 ggcagtttga agcctaggcc cggctcttca acgagttgca cgatccggcc gaagcgcgtc
     3781 accatcccgt gctcagtgat atctaacgtg aagaggctcg caagggccga cagcaccagc
     3841 acggcgatag cggcaggcgt cagcacccgg agcgctcccc tccgtctgcc gcccgcgcgg
     3901 ctcatctgcc aactccaggg tgagctgcct tcatcggttg ccgcccgcgc tgatcggagg
     3961 ctcaaggagc caaaggtcga agtctttgat ggcactcgcc ttcggccgga caagcttgtg
     4021 caccttcggc aacacgtcct cgatcgtctc catctgcaag cggaatgcgg ccaattcggg
     4081 cgcttgccga aacgcctcga cctgtaggct gaacgctagt gcctcgccgc gagcgcggcg
     4141 gacctgttcc tcctggacgg cgagggcacc ctcgaccgtc gccgcggcct cccctttcgc
     4201 ttcgtttacc tcctcggcgg cgaacccctt ggcgcggttg atcgtgcgca gcttgtcctc
     4261 ctgtgcacta gccacgtccc gctcggcgtc gtgtgcctcc tggggcgaat gcacgtagac
     4321 caagcgcacc gagacgagcg cgatgccggc gttcagaccg tctaggcgcg cctgaacgaa
     4381 ctcatgcagt cgcgcctcga cctcggctct ggcgtttgtt agcaacgcgt cgattcgctg
     4441 agtggcaaca acgctacgca gcgctgctcg agtaacgctc cggacgagcg aatcaggctc
     4501 cgccagattt gtgacgtagg ccactggatc gttgacacgg tactgcacgg ttgaccgaac
     4561 atcgatgagg ttggcatcac cggtcaggag gaaggactcg ccctccagca cctcgcgctg
     4621 gaaccaggag cccttggcct tgatcgcctc cggcactgga ttcgagggcc cgcccacagt
     4681 gagccgctcg cgggcgagtg tgcgcgtcgt caagctctcg tccgcctgac tgcggaaacc
     4741 gagctctgca ctccgcgtgc gttcggtttc gacgagacgg tgcgtctcga ttggccaggg
     4801 caggcggtag tgtaggcctg ggccgaggtc ggccgcgacg atgcgagcaa accggaggcg
     4861 caggccgatc tccccggact ggatcgtaaa gaagccgctt gcgagccaga ggaggaccag
     4921 gcttgccaat gccgcgatct taacgcgggt gtcagtaaca aacacgcggg tcgcggccgc
     4981 aaacctgtcg cgcagccgga cccactccgg cggcgcgtgc gtgtgcggcg cgtgcgtgtg
     5041 gagcgagatt tccccggtcc ggagctcacg caccgcctgt cgcaggattt gagcggatga
     5101 ccacaggaac aatcccgcca tgatggcggc cacgatcagg tcgggccact tggtcgcggt
     5161 tgcccaaacg ccgagcgcag cgagcatcac ggcgacgttg ccgatcgcat cgttacgcga
     5221 gcaaagccac acggaccgca cgttggcgtc gccatccttg tagctcacga ggatgacgac
     5281 gctagccacg ttggcagcga gagcca
//
LOCUS       DBNGSB010000189         7730 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_1827397, whole genome shotgun sequence.
ACCESSION   DBNGSB010000189 DBNGSB010000000
VERSION     DBNGSB010000189.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 7730)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7730)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7730
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_1827397"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..82)
                     /locus_tag="AC159B_05455"
     tRNA            complement(6..82)
                     /locus_tag="AC159B_05455"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46..48),aa:Phe,seq:gaa)
     gene            complement(89..166)
                     /locus_tag="AC159B_05460"
     tRNA            complement(89..166)
                     /locus_tag="AC159B_05460"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(129..131),aa:Asp,seq:gtc)
     gene            277..636
                     /locus_tag="AC159B_05465"
     CDS             277..636
                     /locus_tag="AC159B_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrdB family protein"
                     /protein_id="HIR3377226.1"
                     /translation="MASHPLNLALRFLLELAALFALGFWGWTQHSGVRRLVLAIGVPL
                     VAAILWGTFRVPGDPKEAPVPVPGIVRLAFELVFFGAATYALYAADRPQWALVLGVLV
                     LVHYVTSYDRISWLLKH"
     gene            639..1454
                     /locus_tag="AC159B_05470"
     CDS             639..1454
                     /locus_tag="AC159B_05470"
                     /inference="COORDINATES: protein motif:HMM:NF013989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HIR3377227.1"
                     /translation="MAVLPESLNLVYHEAESYPRRIPLEDVYTHIEFPVADRDRPYVY
                     LNMVQTLDGQAVLYGTAYTIGTDVDHYLLRQLRVHADAVLCGAGTLRNDDVIITTHLP
                     LQERRRQRGQSPNPLAVVLTSTCKFGPAVFKKKFFTRTNFEKIIVTTRRATPKDIEKV
                     KRAGVDVAVVRADRSGEVHIPSLMRWFSERGVQRLLCEGGPTLNVALARHSAVDELFM
                     TTALRLGGDPDEPRIFAEPVTDHPLTLVSEFRFEDEGRVKELYLRFRFPRPSS"
     gene            1530..3587
                     /locus_tag="AC159B_05475"
     CDS             1530..3587
                     /locus_tag="AC159B_05475"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR3377228.1"
                     /translation="MSSMGRAQAAAAVVRRLNAAVKSRRLYEKGHTLRTQTVNALHVA
                     VAAYHERFGSFVLETHRNGLIIEGRPFEGGESVDALALMLYSVAVWQLVLLPGITEDE
                     LAEAMDIVTLDRETILNEGGFSALLEKHSLHHARVFEMHPGEGDVGQISLELLLQMLD
                     GSLSPADRAMILGLLRGGPEQAHRLLKVIFERTRQAFPNAEGAELAKHMYAALSALDR
                     LIVDAPHGESQHLFKELAAAVADVDDPKRTDLHAAILNRAAGDLSARALLVAMTSEQI
                     ARMVIPCLETGAVTDPAEQMMRGLPFDPQKARDTLALVSTQTGRSIDIPPALEELRMP
                     EGVRNIRQDLTDYQVTNEAVAVSEDDVRTLVGELHLEEIPVLRDQSLTLLNLVLIDQD
                     EREVEATLEVLTSAVERLLQHSEYELVGVILQHLESTAGDRNAKSRRVQTVLNGLLTA
                     IPSHVTSKDVAQWTDDQPLLMCLKRVGKSAAVALAQALSAERDLSRRQLIAAILSKLG
                     EDIVDSLTPYLKDANPEIVRTVVRILAQLPSQKGLVVLRGVSKHPDAHVRKEMVSALA
                     SVTSPAAQTVVVSFLQDPDLEIREAAFSHLRPDTLRQAQQVLVSMVQSPDLAGRHVFK
                     MRLIRTLADINATEAIPVLRRIGSPFKLLKRDREVARIARQAVERLTGSASSSPRAKG
                     IAS"
     gene            3584..4951
                     /locus_tag="AC159B_05480"
     CDS             3584..4951
                     /locus_tag="AC159B_05480"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR3377229.1"
                     /translation="MSPISRQASVTATLLRYLGTAARFRSIYPADHTLVQRGRGDLLQ
                     VIDLLLRDRESLTFQIYEDTFFLENQMLPEETLRNGALLKACQDRSIGVFTIQRGVSD
                     IEIDAFVSLLILSVQKILTAGGAAAYLKSQNIQHVSVAPPRSSPHGGLEEVKVEPVSA
                     YEAGHIVAQDLQAHAVRRLPLDMKKARVFLSAAIEVVLENRFSLLGLMSHREYNEGSS
                     YHAVNVSILALLIGTNLGLEHKQLMALGMSALVHDIGKVRLPYTLLNRTELTPEEQEQ
                     NDRHPVHGANILRELEGSGRVAAMVALEHHMHYDGSGYPTLPAKTKPHLFSRIVSIVD
                     AYDTLTSARRGTDRPLRPELAMKYIGAGLGTIFSPVIGKVFLKMMGLYPVGSLVELGG
                     SELAIVIRPSDQHVDRPVVQVVKDGRLAHVVDLAADTSQWIATVVDPADAGIEVSSLL
                     SQPAA"
     gene            5240..7132
                     /locus_tag="AC159B_05485"
     CDS             5240..7132
                     /locus_tag="AC159B_05485"
                     /inference="COORDINATES: protein motif:HMM:NF021507.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HIR3377230.1"
                     /translation="MKALIKLLIVLLLLSPAARAQTAPPDIQGHWAEQRILSLFQRNI
                     VTLLLDGTFHPDDEISRGDFISWLVAARGFPPRPVRVPTFGDVPATHPLSPYVEAAVA
                     AGLIAPAPSFMPSASLRRGDAVVLTVQTMGYSVEARIMSRHTIVNGETTALPDHERGS
                     FAIAMLGSPPLLREPSLGARPNAMMTRAEGASLIWAMISAMEAGFSLQISTTVATGLD
                     LVIEKRGVLRARPVWRVQIGAFATRESADRLATMMRERNWAVAIEFQDGVYKVRVGNF
                     TNAAEAALAKDQLSSEGYPSWIVASVSDADALPGPFRTAVVVVDPRLGFRFVPATGDG
                     ERMQPQRTSDLARRTGAAAAINGGFFSTGGDPLGCLMIEGEILSEPDPRRTCAGLDAN
                     GTVLFDHVRLEAAVVNGEVTSRIDGANRLRRADELIVYTPRFDLFTRTNQFGAEVIVS
                     GGVISTVRDLRGNSQIPRDGLVLSGHGRARQWILQNLQPGTPVTIATNIRPASGDPRW
                     AQVRSMIGGGPRLLAAGQFVGGEGFSPTFSDRRHPRSAIGVLADGRVVLVAVDGRQPY
                     HSIGMSLLEFAMELRRIGVVDAMNLDGGGSTTLVVGGQIVNLPADEAGERPVGDALTV
                     MPQSPP"
     gene            complement(7134..>7730)
                     /locus_tag="AC159B_05490"
     CDS             complement(7134..>7730)
                     /locus_tag="AC159B_05490"
                     /inference="COORDINATES: protein motif:HMM:NF024214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR3377231.1"
                     /translation="MTAVSPRIGAVILAAGPSKRMGRPKLLLSYRGVPLLRRAVDAAV
                     AAGCEDTVVVLGAEADQYRAALDGTPARILVNPHFAEGMSGSIQVGIEAMSDDVVAAV
                     IILADQPFIDAGIIRSLIEKYQSSGKKIVASQYGDIRGVPALFDRALFLELLVVTGDL
                     GARTVIETYPGHVATVEIPVSSAEDIDTPEDAERLLKD"
BASE COUNT         1451 a         2426 c         2358 g         1495 t
ORIGIN      
        1 aatcgtggtg ccgggggacg gacttgaacc gccgacgccg ggctcttcag gcccgcgctc
       61 taccaggctg agctaccccg gcacttcttg gcgggagcga cgggagttga acccgcggtc
      121 tcctgcgtga caggcaggtg tgttaaccag gctacactac gcccccgtaa gggaatcgtt
      181 cccgccatgc gaattatacc gggcccgtga gaacagcgtc aatctaacac gccacgcagg
      241 ttccttccct gacactggaa aagaaagcgt ggccacatgg cctctcatcc acttaatctc
      301 gcactacggt ttttgctgga attggcagcg ttattcgccc tcggtttctg gggctggacg
      361 cagcattcag gtgtccggag actcgttctg gccattggtg tgccgctcgt cgccgcgatt
      421 ttatggggga cattccgagt acccggcgat cctaaagagg cgccggttcc tgtcccaggg
      481 atcgtccggc tggcgtttga gttggtgttc ttcggagccg ctacttacgc actgtatgca
      541 gccgatcgtc ctcagtgggc gctcgtcctc ggggtccttg tgctggttca ctacgtgacc
      601 tcatacgacc gcattagctg gttgctcaaa cactaggtat ggcggtgctc cccgagtcgt
      661 tgaacctcgt ctaccatgag gccgaatcgt atccgcgcag gatccccctg gaggatgtct
      721 atacccacat tgagttcccg gtcgcggatc gtgaccgtcc gtacgtctac ttgaacatgg
      781 ttcagacgct agacggtcag gcggttctgt acggtacggc ctatacgatc ggcaccgacg
      841 tggatcacta cctgttgcga cagctccggg ttcacgccga tgctgtactt tgcggggcgg
      901 gaacgctgcg aaacgacgat gtcatcatca ccacccatct gccgcttcag gagcgccgtc
      961 gacagcgcgg tcagtcgccg aacccgctcg cggtggtcct gacctcgacg tgcaaattcg
     1021 gtcccgcggt gttcaagaag aagtttttta cacgaactaa cttcgagaag atcatcgtaa
     1081 ccacaaggcg cgcaacgcct aaagatattg aaaaggtcaa acgtgcgggg gtcgacgtcg
     1141 cagtggttcg ggctgatcgc agcggcgaag tccatatccc gtcgctgatg cggtggttct
     1201 cagagcgagg agtccagcga ctgctgtgcg agggtggacc taccctcaat gtggcgctcg
     1261 cccgtcacag cgcggtcgat gagctcttca tgacaacggc gctgcgtctg ggcggtgatc
     1321 ccgacgagcc gcgtattttt gctgaaccgg tgacagacca tcccttgacg ttggtcagcg
     1381 agttccgctt cgaggacgaa ggacgggtca aagagctgta cctgcgtttt cggttccccc
     1441 ggccttcctc ttaatacccc ttcagcagcc tcgtgtacgg tctgcggtct atggaatttt
     1501 catagaagaa agcgggcatt gacaggggta tgagctcaat gggccgggcg caggctgccg
     1561 ccgcggtcgt tcgaaggctg aacgcggcag tcaaatcccg gcgcctttat gagaagggtc
     1621 atactctgcg cacgcagaca gtgaacgccc tccatgtcgc tgtcgccgcg taccatgagc
     1681 gctttggtag tttcgtgctg gagacccaca gaaacggact catcatcgaa ggccgcccct
     1741 ttgagggtgg ggagtctgtc gacgcgctgg cgctgatgct ctacagcgtc gccgtctggc
     1801 aactcgtctt gctccccggc attaccgaag atgagttggc cgaggccatg gatattgtca
     1861 cgctcgaccg cgagacgatc ctgaacgagg gcggcttctc ggcgctgctc gagaaacaca
     1921 gccttcatca tgcccgcgtg tttgagatgc acccaggcga aggcgacgtc gggcaaatct
     1981 cattggaact ccttctccaa atgctcgatg gatcgctcag tcccgcggac cgcgcgatga
     2041 tcttgggctt gcttcggggg ggacccgagc aagcccatcg cctgctgaaa gtcatctttg
     2101 agcgcacgcg ccaggcattt cccaatgcgg aaggcgccga actcgccaaa catatgtatg
     2161 ccgccctatc cgcgctcgat cgcctcatcg tcgacgcgcc gcacggcgag tcacagcatc
     2221 tgtttaagga actggcagcc gccgtggccg acgtcgacga tccaaaacga acggacctgc
     2281 atgccgcgat cttaaaccga gccgcgggag acctctcggc gcgggcgctc ctggtcgcga
     2341 tgacctcgga acaaatcgcg cgtatggtga taccgtgcct ggagacggga gccgtcaccg
     2401 atcccgcaga gcagatgatg aggggcttgc cgtttgaccc ccaaaaggcg cgcgacactc
     2461 tagcgttggt gtccacgcag accgggcgat cgatcgacat tccgccggcg ctcgaggagc
     2521 tgcggatgcc agagggcgtt cgcaacattc gccaagatct gaccgactat caggtgacca
     2581 atgaggccgt agccgtttca gaagacgacg tccggacact ggtcggcgag ttgcatcttg
     2641 aagaaatacc ggtactgcgt gatcaatcac tcacgttgtt gaatctcgtc ctcattgacc
     2701 aggacgagcg agaagtcgag gccaccctcg aagtgctcac ctccgccgtg gaacggctgc
     2761 tccagcacag cgagtatgag ctggtcgggg taatcctcca gcacctcgag tcgacggctg
     2821 gcgatcgcaa cgccaagtcc cgccgcgtgc agaccgtgct gaacgggctg ctcaccgcca
     2881 tcccgagtca tgtgacatca aaagacgtcg cacaatggac cgacgaccag cccctcctga
     2941 tgtgcctcaa acgcgtgggc aaatcggcag ccgtggcgct ggcgcaggcg ctgtcggccg
     3001 agcgcgatct ctccaggcgg cagctgattg cagcgattct cagtaagctc ggggaagata
     3061 tcgtggattc cctcactccc tatctcaagg acgccaatcc tgagatcgtc cgcacggtcg
     3121 tgcggatcct tgctcaattg ccatcccaaa agggactggt cgtgctgcgc ggggtcagta
     3181 agcatcccga tgcccacgtg cgaaaagaaa tggtgagcgc gctcgcatcg gtgacatccc
     3241 cggcagctca aaccgtcgtg gtgtcgttct tgcaggatcc cgatctcgaa atccgggagg
     3301 cggcgttctc gcacttgcgg cccgacacat tgcgccaggc gcagcaggtc cttgtctcga
     3361 tggtgcagag ccccgacctc gccggacggc atgtgttcaa gatgcggctc atccgaacgc
     3421 tcgcggacat caacgccacg gaagcgattc ccgtcctccg gcggatcggc agtccattca
     3481 agctgctgaa gcgggaccgt gaagttgcgc gcatcgcgcg ccaggcggtc gagcggttga
     3541 cggggagcgc gtcgtcgtcc ccgcgggcca aaggcatcgc gtcatgagcc cgatctcccg
     3601 gcaggcctcc gtcaccgcca cactattgcg gtatctgggc accgcagcta gatttcgcag
     3661 catctatccc gcggatcata cgcttgtcca gcggggacgc ggggatcttc tccaggtcat
     3721 cgatctgctg ctccgcgatc gtgaatcatt gacgttccag atctacgaag acaccttctt
     3781 cctcgaaaat cagatgctgc ccgaagagac gcttcggaac ggcgccctgc tgaaagcctg
     3841 ccaggatcgg agcatcggcg tgtttacaat ccagcgcggc gtttccgaca tcgagatcga
     3901 tgccttcgtc agcctgctca tcctctccgt gcagaaaatc ctgacagccg gaggagcggc
     3961 agcatacctc aagagccaga acatccagca tgtttcggtc gcgccaccgc gctcatcgcc
     4021 gcacgggggt ctggaagagg tgaaagtgga gccggtcagc gcctacgagg ccggacacat
     4081 cgtcgcacaa gatcttcagg cgcatgccgt gcggcgcctg ccccttgata tgaagaaggc
     4141 gcgagtattc ctgtcggcgg cgatcgaagt cgtgctcgag aaccgattct cgttgctcgg
     4201 actcatgtca caccgtgagt acaatgaggg cagcagttat cacgccgtca acgtgtcgat
     4261 cctggccctg ttgatcggga cgaacctggg gctcgagcat aaacaactga tggcgctggg
     4321 catgagcgcg ctcgtgcacg acatcggcaa ggtgcgactg ccctacacac tattgaaccg
     4381 gacggagctg acgccagagg aacaggagca gaacgaccgg catcccgtgc atggcgccaa
     4441 tatcctgcgg gagctcgagg gttcgggccg ggtcgctgcg atggtcgcac tcgagcacca
     4501 catgcactat gatggctcgg gctatccgac gctgcctgca aagaccaagc cgcacctgtt
     4561 ctcgcgcatc gtctccatcg tggatgccta cgacaccctc acttctgcac gccgtggcac
     4621 cgaccggccg ctgcggccgg agctcgcgat gaagtatatc ggcgctgggc tgggcacgat
     4681 tttttctccc gtcatcggga aggtcttcct gaagatgatg gggctctatc cggtgggcag
     4741 cctggtggag ctgggcggca gcgagctggc catcgtgatc cggccgtcgg atcagcacgt
     4801 cgaccggccg gtcgtgcagg tcgtcaagga tggccggctg gcacacgtcg ttgatcttgc
     4861 cgccgacact tcacagtgga tcgccaccgt cgtcgatccc gccgacgccg gcatcgaggt
     4921 cagctcattg ctgtctcaac ctgcggcttg atcatgaaag acacatcaca aaaggcaaga
     4981 ggtagcactc cggatgctac ctcttgttca acgcagaagc actgcgtctt actacttctt
     5041 cttcttgccc ttggccttct ttctcgccat tggtcatcct ccctctcgcg ctcccgcgcg
     5101 cgtatctgga cggctttctt gccgtcgcgc acgatagttc gccaccatat gtagtttacc
     5161 tcccctgatg ctccacaatc cttcgtgggt caggtcgcgc gaggtgcgca agggacgaat
     5221 cgggaaaaca tacccaggga tgaaggctct catcaaactc ctcatcgtgc tcttgctgct
     5281 ttcgccggcg gcgcgcgcgc aaacggcccc tcccgacatt cagggacact gggcagaaca
     5341 acgcattctc tctctgttcc agcgaaacat cgtcacgctg cttctcgacg gcacgttcca
     5401 ccctgatgac gagatctcgc ggggcgactt tatctcatgg ctggttgcag cgcgcggctt
     5461 cccaccccgt cccgttcgcg tgccgacatt cggcgacgtg cctgcgaccc acccgctatc
     5521 tccctacgtt gaagctgcgg tagccgcagg cctgatcgca ccggcgccat ccttcatgcc
     5581 gtccgcatca ctccgtcgcg gcgatgccgt ggtgctcacc gtccagacga tggggtatag
     5641 cgtcgaggcc cggatcatgt cccggcacac aatcgtgaac ggggagacca ctgccctgcc
     5701 cgatcacgaa cggggatcgt tcgcaattgc gatgctcggc tcgcctccac tgctccgaga
     5761 gccctcgctc ggagcgcgcc cgaatgcgat gatgacgcgc gccgagggcg ccagcctgat
     5821 ctgggcaatg atatctgcga tggaggcggg atttagcctg cagatctcta cgacggtcgc
     5881 gacaggcctt gatctcgtca tcgagaagcg cggcgtgttg cgggcgaggc cggtctggcg
     5941 tgtccagatc ggcgccttcg ccacgcgaga gagcgcggac aggctcgcta ccatgatgcg
     6001 agaacgaaac tgggccgtgg cgatcgagtt tcaggatggg gtctacaaag tgcgcgtcgg
     6061 gaacttcaca aacgccgcgg aagctgcgct ggcaaaagat cagttgtcct ccgaaggcta
     6121 tccaagctgg atcgtcgcgt cggtctcgga cgcagatgcg cttcccggcc cgttccgaac
     6181 cgcggtcgtt gtcgtggatc cgcgcctggg gttcaggttc gttcccgcga ccggcgacgg
     6241 tgagcgcatg cagcctcagc ggacaagcga tttggcacgc cgaacgggag cggcggcagc
     6301 gatcaatggg ggatttttct ccacaggtgg cgatccgctg ggatgcctga tgattgaagg
     6361 cgagattctc agcgagcccg acccccggcg cacgtgcgcg ggattggacg ccaacggcac
     6421 ggtcctcttt gaccacgtgc gtctggaggc cgccgtagtg aatggtgagg tcacgtcgcg
     6481 gattgacggt gcgaaccgac tccgccgcgc cgacgagctc atcgtgtaca cgccgaggtt
     6541 cgatctcttc acgcgcacga accagttcgg ggccgaggtg attgtctccg gcggtgtcat
     6601 ctcgacggtt cgcgacctca ggggcaacag tcagatcccg cgcgacggac tggtcctctc
     6661 aggacacggt cgcgcgcgcc agtggattct gcagaacctg caacccggca cgccggtgac
     6721 gattgcgacc aacatcaggc ccgcgtccgg cgatccccgg tgggcccagg tgcgttcaat
     6781 gatcggcggc ggtccgcggc tgctggcggc aggacagttc gtcggcggcg aaggattctc
     6841 gcccacgttc agcgaccggc gccatccacg cagcgcgatc ggtgtgctgg ccgatggacg
     6901 ggtggtgctc gtcgcggtcg acggccggca accctatcac agcatcggca tgtcgctgct
     6961 ggaattcgcg atggagctgc gacggattgg cgtcgtcgac gcgatgaacc tcgacggcgg
     7021 gggctccacg acgctggtgg tgggcggaca gatcgtcaat ctccccgccg atgaagcagg
     7081 cgaacggccc gtcggcgacg cactgacagt aatgccgcaa agcccaccct aggctagtcc
     7141 ttgagcaggc gctccgcgtc ttcgggcgtg tcgatatctt ccgcagatga caccgggatc
     7201 tccactgtgg cgacatggcc agggtacgtt tcgatcaccg tccgcgcgcc tagatccccg
     7261 gtgacgacga gcaactccag aaagagggcg cggtcaaaaa gagccggcac cccccgaatg
     7321 tcgccatact gactggccac tattttcttc cccgaactct gatatttctc gatcaaactc
     7381 cggatgatgc cggcatcgat gaagggttgg tctgcgagta tgatcactgc agcgacgacg
     7441 tcatcgctca tcgcctcaat acccacctga atcgagcccg acatcccttc ggcgaaatgc
     7501 gggttgacca gaatccgggc aggtgtcccg tcgagcgcgg cgcggtattg atccgcctcg
     7561 gcgccgagca cgacgaccgt gtcttcgcac ccagcggcaa ccgcagcgtc tacggcgcgc
     7621 cgcagcagcg gcacgcccct gtaggacagc aggagtttgg gccggcccat gcgcttgctc
     7681 ggaccggcgg ccaggatgac ggcgccaatt ctaggcgaga ctgcggtcat
//
LOCUS       DBNGSB010000190         3566 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3053394, whole genome shotgun sequence.
ACCESSION   DBNGSB010000190 DBNGSB010000000
VERSION     DBNGSB010000190.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3053394"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="AC159B_05495"
     CDS             complement(<1..328)
                     /locus_tag="AC159B_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase family protein"
                     /protein_id="HIR3377232.1"
                     /translation="MPITIEEAIARIPQWAEASAIGVALLPGGISNFNYRVDVDGESF
                     VVRIAAQDTALFGINRQREYRCTVAASQTGVAPEVITFLPDVEVLVTRFISGRCPSTA
                     EMTSPEM"
     gene            complement(352..1530)
                     /locus_tag="AC159B_05500"
     CDS             complement(352..1530)
                     /locus_tag="AC159B_05500"
                     /inference="COORDINATES: protein motif:HMM:NF013720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HIR3377233.1"
                     /translation="MAGRDAYDYRLVHSMRYQKSPLFSSFRKLGATSFLWYNHLLHAV
                     DFEGQSKEQEYGAIRNAVTLWDVGVERPVEIDGPDGFALMDLLTPRDMRRCMIGQCKY
                     ALITDENGCVVNDPVVLRLGENRFWLCPSDSDVHLWAKGVAVGAGLRATVRLSDVYPV
                     QVQGPKSPDVMRAIVGDPVRKLRYYWWMEAQLPGKIPVIITRTGWTGEVGYEVFLLDN
                     SRADEMVTLIMERGKPFGLIPAGPNHARRVEAAILNNQADMDLTTNLYELGLEWQIEP
                     KERFIGKEALERIRREGLKRKLVGFEVDAAAGPHEFEGPWSVHKDGARVGNASAFVFS
                     PTLKKNIGLAMVKIEHTAVGERFEVQRLPGEPRYSAKVVSLPFIDPNKEIPKRRLDVS
                     "
     gene            1637..>3566
                     /locus_tag="AC159B_05505"
     CDS             1637..>3566
                     /locus_tag="AC159B_05505"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcvT family protein"
                     /protein_id="HIR3377234.1"
                     /translation="MRDEAQAVIIGGGVTGCSIAYHLTQMGWRDIVLVERGTLTGGST
                     HRAAGLIGQLRGTFNLTRMIMYSVELYGRLLQETGIDPDWRQVGSLRLASSTLRMEEI
                     RRMVAQAKAFGLDVELLTPREALAMCPIIGDRDLVGAAHIPTDGRIDPSGLAYALAHG
                     ARSRGCEMHTQTAVTGIEVRHGRVHAVTTPRGVIRTPVVVCASGVWSWHVGRMIGVAV
                     PIVPIEHHYMMTVPFEVPRNLPTFRDPDLRFYGREEVGGLLVGGYEGNPVRCGPEPIP
                     ADLDPVKMVPNWDRFETLARNAAIRIPALNDVGIRKLLIGPETFTPDGDFLLGESPDV
                     RGFFAAGGTPGIAAGGGVGKAMAEWIIEGRPSQDLWRADIRRFGRHYADRAYAADRAV
                     EVYARNYTVHLPMEEFESVRPLRTSAPYDRLVALGAVLGEKAGWERPNWFKVNEGGDP
                     HGPAPGGWIRHNWSPAIAVEHTATRESAGIFDFSSFSKFEVQGPGALGALQWLTDNDL
                     DKPPGSVTYTQMLNTRGGVECDLTITRLQSNQFFIVTGSAFGVHDLAWIRTHLPQDGS
                     VQARDVTTEFACIGLWGPQAREILQDISGGELSNGAFPDMTDRPLDSAGAQVRALRVT
                     YVGDLGWDLYMPVPDGRAV"
BASE COUNT          666 a         1142 c         1124 g          634 t
ORIGIN      
        1 ccatctcagg gctggtcatc tcggctgtgg aggggcagcg tccggagatg aatcgcgtga
       61 ccagaacttc cacgtccggc aggaaagtga tgacctcagg ggccacgccg gtctggctcg
      121 ccgcgacggt gcaccgatac tcccgctgac ggttaatgcc aaagagcgct gtgtcctggg
      181 cggcgatccg tacgacgaag gattccccat ccacatcgac tcgatagttg aagttggaaa
      241 tgccgcccgg caacaatgcg acgccgattg cggaggcctc tgcccattgg ggaatgcggg
      301 cgatggcctc ctcgatggtg atgggcatga cgccggtcga gctctgcgcc gtcaggaaac
      361 gtccagccgg cgcttgggga tctccttgtt cgggtcgatg aacggcagcg atacgacctt
      421 cgcagagtac cgcggctcgc cgggcagacg ctgtacttcg aaccgctcac ctaccgcggt
      481 gtgctcgatc ttgaccatgg ccagcccaat attcttcttg agggtggggg agaagacaaa
      541 cgccgaagcg ttgccgaccc gtgccccgtc tttgtgcacg gaccaggggc cttcaaactc
      601 gtgtggacct gccgcggcgt cgacctcgaa ccccaccagc tttcgcttga gcccctcccg
      661 ccggatgcgc tcgagcgcct ctttgccgat gaaccgctcc ttgggttcga tctgccattc
      721 cagacccagt tcatacaggt tggtcgtgag gtccatgtct gcctgattgt tgagaatcgc
      781 ggcctcgacg cggcgcgcgt gattagggcc ggccgggatg aggccgaacg gcttgccgcg
      841 ctccatgatc agggtgacca tctcatcggc gcggctgttg tccaggagga acacctcata
      901 gccaacttca cccgtccagc cggtgcgcgt gatgatcacg gggatcttgc cgggcaattg
      961 ggcttccatc caccagtaat agcgcagctt cctgacaggg tcgccgacga tggcccgcat
     1021 cacgtccggg gatttcggtc cctggacctg caccggatag acatcgctga gccgcacagt
     1081 ggcgcggagg cccgcgccga ctgccacacc cttggcccac aggtggacgt cactgtcaga
     1141 aggacagagc caaaaacgat tctcccccag gcgcaacacg acgggatcat tcaccacaca
     1201 tccattctcg tcggtgatga gggcgtactt acactgaccg atcatgcacc ggcgcatgtc
     1261 gcggggcgtg agcaaatcca tgagcgcgaa tccgtcgggt ccgtcgatct ccaccggccg
     1321 ttcaaccccg acgtcccaga gcgtcaccgc attccggatc gccccgtact cctgctcctt
     1381 agattgcccc tcgaaatcga ccgcgtggag caaatgattg taccagagga atgatgtcgc
     1441 tcctaacttt ctaaaactcg aaaacagcgg cgatttctga tatcgcatcg agtgcacaag
     1501 gcggtaatcg tacgcgtcac gaccagccat cgattcaatc ctcctcgggt ggagaatatt
     1561 tttgctcccg cccttatcgt gacctgcacg cagccagagg attgactggc gagcgggcgc
     1621 gaatcagatt cagacaatgc gtgacgaagc ccaggcagtc attattggcg ggggcgtgac
     1681 cggctgcagc atcgcgtacc atctgaccca gatgggatgg cgcgacatcg tgcttgtgga
     1741 gcggggcacc ctcaccgggg ggtcgaccca ccgcgccgcc ggactcatcg gccagctccg
     1801 agggaccttc aacctcacac ggatgatcat gtacagcgtc gagctgtacg gtcgcttgtt
     1861 gcaagaaacc gggatcgatc cggactggcg gcaggtgggg agcctgcgac tggccagcag
     1921 cacactgcgc atggaagaga tccgccgcat ggtggcccag gcgaaggcat tcggcctcga
     1981 cgtcgaactg ctcacgcccc gcgaagccct cgccatgtgc cccattatcg gcgaccgcga
     2041 tctcgtgggc gcggctcaca tccctacaga cggacgcatt gaccccagcg ggctggccta
     2101 cgcgcttgcg cacggcgcgc gcagccgggg ctgcgagatg catacgcaga ctgcagtaac
     2161 cggcatcgaa gtgcgccatg gccgcgtgca cgccgtgaca acaccgcgcg gggtgattcg
     2221 gacgcccgtc gtcgtatgcg cgtcgggcgt gtggtcctgg cacgtcggcc ggatgatcgg
     2281 agtcgcagtc ccgatcgttc cgatcgagca tcactacatg atgacggtgc ccttcgaagt
     2341 gccgcggaac ctgccgacgt ttcgcgatcc ggatctgcgc ttctacggcc gcgaggaggt
     2401 cgggggcctg ctggtgggcg gatatgaggg aaaccccgtt cgctgcggac cggagcccat
     2461 cccggcagac ctggatcccg taaaaatggt gccgaactgg gaccgcttcg agacgctggc
     2521 gcgcaacgcg gcgatccgca tccctgcact caacgacgtg ggcatccgca agttgctgat
     2581 cggaccggag acctttactc ccgacggcga tttcctgttg ggtgagtctc cagacgttcg
     2641 cgggtttttc gcagccgggg ggaccccggg aatcgccgcc ggtggcggtg tgggaaaggc
     2701 catggcggag tggatcatcg aagggcgtcc cagccaggat ctatggaggg ccgacatccg
     2761 gcggttcgga cggcactatg ccgaccgcgc ctacgccgcc gatcgggccg tcgaagtgta
     2821 cgcccgaaac tatactgtgc acctaccgat ggaagaattc gaatcggtcc gtcccttgcg
     2881 caccagcgcg ccatacgatc gcctggtcgc cctgggtgcg gtattagggg agaaggccgg
     2941 atgggagcga cccaactggt tcaaggtcaa cgagggcggc gatccccacg gcccggcacc
     3001 aggtggatgg atcaggcaca actggtcgcc ggccattgcg gtcgagcata ccgcaacgcg
     3061 agaaagcgcc ggcatcttcg atttctcctc cttcagcaaa tttgaagtgc aggggcccgg
     3121 ggcgcttggc gcgctgcaat ggctcaccga taacgatctg gacaagccgc ccggatcggt
     3181 cacgtacaca cagatgctca acacgcgcgg cggcgtcgaa tgcgacctca cgatcacgcg
     3241 cctgcaatcg aaccagttct tcatcgtgac cgggagcgcg ttcggtgtgc acgacctcgc
     3301 ctggatccgc acccacctcc ctcaggatgg ttcagtgcag gcgcgggacg tcacgaccga
     3361 gttcgcatgt atcggtctgt ggggccctca ggcgagggaa attctccagg acatcagcgg
     3421 gggcgagctc tccaacggcg cgttccccga catgacggac cgccccctcg acagtgccgg
     3481 cgcccaggtg cgcgcgctgc gcgtcacgta cgtaggagac ctcggctggg acctctacat
     3541 gcccgtccca gacggacggg cggtct
//
LOCUS       DBNGSB010000191         8761 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_601417, whole genome shotgun sequence.
ACCESSION   DBNGSB010000191 DBNGSB010000000
VERSION     DBNGSB010000191.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 8761)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8761)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8761
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_601417"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="AC159B_05510"
     CDS             complement(<1..794)
                     /locus_tag="AC159B_05510"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR3377235.1"
                     /translation="MTSRSKVIIFTAVFVVLATAAAVTLTAGMKRSTAVAARVNGEQI
                     YATDLDTEVDAIARQYGIPDDDKQRTEIIRIVLDQMIEQRIINQEARRYDAVASEAQI
                     STQLADIRRNFPSDAEFRQALEQRRWTLADLKNRLRGTITMQNLTERVTTITVTDAEI
                     QKYFNEHRSEYDQAEQVRVSHILLETEAEARLVLARLRRGEKFGELAQQYSKDPGSKA
                     QGGDLGFVSRGQLVGEFEKAAFSLKPGQTSGIVKTQFGFHIIRVTD"
     gene            complement(845..4153)
                     /gene="mfd"
                     /locus_tag="AC159B_05515"
     CDS             complement(845..4153)
                     /gene="mfd"
                     /locus_tag="AC159B_05515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HIR3377236.1"
                     /translation="MSLSALLPVLREQPSYVQMLASLRAQRRPWVVGPGSSEKAYLLA
                     ALGDGLHLIVNGSVLIITPTRDAAERLHDDLLAFVPQLESRLMLFPHWETQGPNEGKI
                     AAEALGERVAILQRLRDETSVWVVAPVEALLRRVPSPEELLASARRIRKGERADRDQI
                     AAFLAGSGYERLDLVAEKGQFAIRGGILDVFSPDASAPARLEWFGDEVESIRLFDLET
                     QRSIQDTNDVVLHPAVSASGNATLLDYLPASSLLVLDEPFDLERQAKVAVERGAELEP
                     EAFISWTDLQKRLHTASLRISSVHPPDDRAEEIAMHFGGVEAFGGQMKLLARAIESWL
                     NHGRRVVVATAQAERITEILRGHGIQPASTGSLARVPEPGRAIVVGAPLSRGFQVDAA
                     ALVVLTDSEIVGWRRRRRRFRFREGVRLYSWTDLSPGDYVVHLHHGIGNYRGMVRLLL
                     NGAERDYLELEYAQNDKLYVPTDQITLVQRYIGVEGQAPKVHRLGGTEWEREKKRVKE
                     AAQEMARELLTLYASRESAAGHAFGPDTPWQHELEATFEFEETPDQWLAIQDIKRDME
                     TAKPMDRLIAGDVGYGKTEVALRAAFKAVMDGKQVAVLVPTTILAQQHYNVFLKRLSA
                     YPIKVEMLSRFRSASEQKRVIEQAKVGQVDVLIGTHRLLQKDVTFKNLGLVIIDEEQR
                     FGVRHKEKLKQLRATVDVLTLTATPIPRTLHMSLVGLRDMSVMETPPDARLPIQTEIR
                     PDEDTLIHDAVLREMERGGQIYVVHNRVQSIERAARRIRALVPEANVAVAHGQMPEEK
                     LEEVMMDFLGGRFNVLVCTTIVEIGLDIPQVNTIIIEDAHMMGLSQLYQLRGRVGRSD
                     RQAFCYLLYPRGATLSEEARQRLEAMQEFVELGSGFKLAMRDLEIRGAGNLLGSEQHG
                     HLAAVGFELYSQLLDEAIRELRGEMIEEVPDATIDLGIDAYLPNEYISDEGHRMAVYR
                     KLASARTVDDANAVVEEVTDRFGMLPEQATNLIEVVRLRALAKQAGVVSITREQGRIL
                     IKPAAGWTPSPEDEARLASALRGRLTMTPTGLRLRTSGTRFSEDAGLIRQTLLALARS
                     GRRREATAVASGSS"
     gene            complement(4220..5959)
                     /locus_tag="AC159B_05520"
     CDS             complement(4220..5959)
                     /locus_tag="AC159B_05520"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HIR3377237.1"
                     /translation="MLRVGMAQLNLVVGDLEGNTRRICEAIDHAKDLGVDLVCFPELS
                     VPGYPPEDLLLKPDFVRGNLAAVGEITRRTHGIAAVVGFVDQDAHLYNAAAICVDGER
                     VGVYHKQRLPNYGVFDEKRYFYPGRRAGVYVINGVPVGVTICEDIWAPGGTCQAEAAE
                     GALVIININGSPYHAGKWRGRAEMLGTRARDYRVVLCYNNLVGGQDELVFDGMGMIFD
                     HTGTLLARGRQFDEDFIVSDIDPEAVRRHRMFSPVPPVEGEDRDIESFTIHISDAPAR
                     PKTPRKATLEPPDEPSEEVYRALVLGTRDYVRKNGFRDVVIGLSGGIDSALVATIACD
                     ALGNEHVHTAFLPARFTSAESGRYAFEVAQNLDVQMIDMGIDGIFQAFLAALQPVSAG
                     RPPDVTEENVQARIRGTLLMALSNKFGWLVLTTGNKSEMSVGYATLYGDMAGGFAVIK
                     DVPKTLVYELANWRNRRQAVIPEGVITRAPTAELRPDQKDTDTLPPYEVLDPILKMYV
                     EEDIPPQEIVAAGYPTDLVNRIIAMVDRNEYKRRQAPPGVKITPKAFGKDRRLPITNW
                     YRSTTQHRVIASD"
     gene            complement(6270..6872)
                     /locus_tag="AC159B_05525"
     CDS             complement(6270..6872)
                     /locus_tag="AC159B_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing response regulator"
                     /protein_id="HIR3377238.1"
                     /translation="MPQNSTKAIRILVADDEAVIRLGLRTMLEAQGYRVVGEAADGSR
                     ALDLVSRLKPDLVFLDIKMPGVDGVHAARRLQRDGNTPVVILTAYSDREFVDSAKGAG
                     VLAYLVKPVRESDLRPAIEVAMSRFRDIRALRGEVEDLENSLETRKLVERAKGVLMRK
                     LGIQEAEAFTRIQRESRNTRRPMKEIAQQIISTENTRPSP"
     gene            complement(6895..8526)
                     /locus_tag="AC159B_05530"
     CDS             complement(6895..8526)
                     /locus_tag="AC159B_05530"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3377239.1"
                     /translation="MTSQKTAEISALRAISRAIGEALDLDTTLHLITKTTAEVMGMDS
                     CSIYLLDPSGEYLVLKATTGLAEGAVGRARLRFGEGLTGWAAKEGEAVASSDAARDPR
                     FVYLPETRESTFRSLAAVPLVTAGRVLGAVNVQTTARHEYSNNEMELLGVIADLASGA
                     IEKATLYDNMRRQIMELSTLAEVSETITSPLYLEEILRLIVEMAARMLTAKTASLMLI
                     DEDSGELVIAAAQSAGAAYLKRRHLKVGEGIAGLVAKDGKPIAVTDVLTEPRFVTKEV
                     AQQEGLRALLSVPLVVRDKVIGVFNCYKDRPYRFTDAEVKLLTTLANQTALAIENANL
                     VVRSAIIREMHHRVKNNLQTVAMLLRLQLRDGREVSGREVLSETINRVLSIAAVHEIL
                     SVEGFRLINLRQLLDRVAQNVTQTMAQPGRALTVSVTGDDIYLNSQQATSLALAVNEL
                     LQNAVEHAFADRADGHVEIRLAQREGKLVLQVQDNGAGLPANFDPMAGDDLGLKIVHA
                     LVAEDLGGSLTFTNKTGTTVTITIPNISASGAGRQ"
BASE COUNT         1577 a         2751 c         2669 g         1764 t
ORIGIN      
        1 ctgtcggtga cacggataat gtgaaaaccg aactgcgtct tgacgattcc tgacgtctgt
       61 ccgggcttca gtgagaacgc tgccttctca aactcaccga ccagctggcc gcggctgacg
      121 aatcctaggt cgccgccctg cgccttgctt cccggatcct tcgagtattg ctgggcgagt
      181 tcgccaaact tctcaccgcg ccggagtcgc gccaggacca gccgcgcctc ggcttccgtc
      241 tcgagaagaa tgtggctgac gcggacctgc tcagcctgat cgtactcgct gcggtgttca
      301 ttgaagtact tttggatttc cgcgtctgtc accgtgatgg ttgtcacgcg ctcagtcaga
      361 ttctgcatgg tgatcgtgcc ccggagtctg ttcttcagat ccgccagcgt ccaccggcgt
      421 tgctccagcg cctgccggaa ttcagcgtcc gagggaaagt tgcgcctgat gtctgccagt
      481 tgcgtgctga tttgcgcctc cgaggcgacg gcgtcgtagc ggcgggcttc ctggttgatg
      541 atccgctgtt cgatcatctg atccaggacg atccgaatga tctcggtgcg ctgtttgtcg
      601 tcgtcgggga tgccatactg cctcgcgatg gcgtccactt ccgtgtcaag gtccgtcgca
      661 tagatctgct cgccgttcac cctggcggcc acggcagtgc tgcgcttcat ccccgccgtc
      721 aaagtgacgg ccgccgccgt cgcgagcaca acgaaaacgg cggtaaagat gattacttta
      781 gaacgactcg tcattcgcga ctcctcccgg tgtcagtacg caccggctgt cgaaagttta
      841 ctctctatga ggaccctgat gctacggcgg tcgcctcgcg ccgccgcccg ctcctggcaa
      901 gcgcgagcaa tgtctgtctg atcaatcccg cgtcctcaga gaaccgcgtt ccgctcgtgc
      961 ggaggcgcag acctgtcggg gtcatggtca gccggccgcg gagcgctgat gccagacgag
     1021 cttcatcttc ggggctcggc gtccatcccg ccgcgggttt gatgagaata cgcccttgct
     1081 cgcgcgtgat ggacactact ccggcctgct tggcgagcgc gcggagtcgc accacctcga
     1141 tcaggttggt cgcctgctcg gggagcatcc cgaaccggtc cgtcacctcc tcgaccacgg
     1201 cattggcgtc gtcgacggtg cgcgcgcttg cgagctttct gtatacggcc atgcggtggc
     1261 cctcgtctga gatgtactcg ttgggaagat acgcatcgat cccaagatca atcgtggcgt
     1321 cggggacctc ttctatcatc tccccgcgca gctcacggat cgcctcatcc agcagctggc
     1381 tgtacagttc gaaccccacg gcggccaggt gcccgtgctg ctcagaaccg agcaggttgc
     1441 cggccccgcg gatttccagg tctcgcatcg ccagtttgaa gccgctgccc agctcgacga
     1501 actcctgcat ggcttcgagc cgctgccgcg cctcttcgct caaggtcgcc ccgcgaggat
     1561 acagcaggta gcagaacgcc tgccggtcgg agcgtccgac gcgaccccgc agttggtaga
     1621 gctgcgacag ccccatcatg tgggcgtctt cgatgatgat ggtattcacc tgtgggatgt
     1681 cgaggccgat ttccacgatg gtcgtgcaga cgagcacatt gaagcgtccg cccaagaagt
     1741 ccatcatcac ttcttccagt ttttcctctg gcatctggcc gtgtgccacc gcgacgttcg
     1801 cttccggcac gagggcgcgg atccgccgcg ccgcccgctc aatgctctgg acccgattgt
     1861 gcaccacata gatctgaccg ccgcgctcca tctcgcgcag aaccgcgtcg tggatcaagg
     1921 tatcctcgtc gggccggatc tccgtctgga tcggcaggcg cgcgtccggc ggcgtctcca
     1981 ttaccgacat gtcgcgcaat cccacgagcg acatgtgcag agtgcgcggg attggggttg
     2041 cggtgagcgt caacacgtcg acggtcgcgc gaagctgctt gagcttttcc ttgtgcctga
     2101 ccccgaagcg ctgctcttca tcaataatca ccaggcccag gttcttgaag gtgacgtcct
     2161 tctgtaagag ccggtgcgtc ccgatgagga cgtcgacctg tccgaccttg gcctgttcga
     2221 tgacccgctt ctgttccgaa gccgaacgga accggctgag catctccacc ttgatgggat
     2281 aggcagataa gcgcttcagg aacacgttgt aatgctgttg agccaggatt gttgtgggca
     2341 cgagcacggc gacctgcttg ccgtccatca ccgccttaaa agccgcgcgc aatgctactt
     2401 cggttttccc gtatccgaca tcaccggcga tgagccggtc catcggcttt gcggtctcca
     2461 tatcacgctt gatatcctga atggcgagcc actgatccgg tgtttcttcg aactcgaagg
     2521 tcgcttcgag ctcgtgctgc cagggggtgt cgggaccgaa ggcatggccc gccgccgact
     2581 cgcggctggc atacagggtg aggagctcgc gcgccatctc ctgcgccgct tctttgacgc
     2641 gtttcttttc acgttcccac tcggtgccgc ccaggcgatg aaccttgggg gcctgaccct
     2701 ccacgccgat ataccgctgc accagagtaa tctggtcggt cggaacgtac agcttgtcgt
     2761 tttgcgcgta ttcgagctcg agatagtcac gttccgcgcc atttaacaac aggcggacca
     2821 taccgcggta gttcccgatg ccgtggtgca gatgcacgac gtaatcaccg ggcgacaagt
     2881 cggtccacga gtagaggcgg acgccttccc ggaagcggaa ccgtcgtcgc cggcgacgcc
     2941 agccgacgat ttcactgtcg gtcagcacga caagcgccgc cgcgtccacc tgaaacccgc
     3001 gcgacagagg cgccccgacg acaatcgcgc gcccgggttc gggaactctc gcgagcgagc
     3061 cggtcgacgc cggttgaatc ccgtggcctc gcaggatctc tgtgattcgt tccgcctgcg
     3121 ccgtggcgac gacgacgcgc cgcccgtggt tcagccacga ctcgatagcc cgcgccagca
     3181 atttcatctg cccgccgaac gcttccaccc cgccaaagtg catcgcgatc tcttccgcac
     3241 ggtcgtcggg agggtgcacc gatgaaatcc ggagcgacgc ggtatgcagc cgcttctgga
     3301 gatcagtcca gctgatgaac gcctccggtt cgagttcggc tccccgctcg acggccactt
     3361 tcgcctgacg ctcgagatca aacggttcgt ccaggaccag gagcgaagac gccggcagat
     3421 agtcgagcaa cgtcgcgttc ccgctagcgg aaacggcagg atggaggacc acatcattcg
     3481 tgtcctgtat gctgcgctgc gtttccaggt cgaagaggcg aatcgactcg acctcatccc
     3541 cgaaccactc cagacgcgcc ggcgcactgg cgtccggcga gaatacgtcg aggatgccgc
     3601 cgcgaatggc aaactggccc ttctcggcga cgaggtcgag ccgctcgtag cccgatccgg
     3661 ccaggaacgc ggcgatttga tcgcggtccg ctctctctcc tttgcgaatg cggcgggcgg
     3721 atgcgaggag ttcttccgga gaggggacgc gtcgaagcag cgcctcgacc ggcgccacga
     3781 cccacacgga cgtctcgtca cgcagccgct gcaggatcgc cacccgctca cccagagcct
     3841 cggccgctat cttgccttca ttcggaccct gcgtttccca gtgcgggaag agcatcaggc
     3901 gggactcgag ctgtggaaca aaagccagca gatcgtcatg gagccgttct gcggcatcac
     3961 gggtgggtgt gatgatcagg acactgccgt tgacgatcaa atggagaccg tcgccgagag
     4021 cagcgagaag gtacgccttc tccgaggagc cgggaccgac gacccacggc cgccgctggg
     4081 cccgcagtga ggcaagcatt tgcacatatg agggctgctc ccgcaatacg ggaagcagcg
     4141 cggagagtga catcgcaaca cactattgta tcgaagcagc gactcgaact acaagacaaa
     4201 cggctacaat ctgcggaaac tagtcggagg cgatcacgcg gtgctgcgtg gtgctgcgat
     4261 accagtttgt aatcggcaac cggcggtctt tgccaaatgc tttcggcgtg atcttcacgc
     4321 ccggaggtgc ttgtcgccgc ttgtattcgt tgcgatccac catcgcgatg atgcggttga
     4381 cgagatccgt cggataccct gcggcgacga tctcctgcgg agggatgtcc tcttctacgt
     4441 acatcttcag aatgggatcg agcacttcgt agggcggcaa tgtgtccgtg tctttctgat
     4501 cggggcgcag ctcggcggtc ggcgcacggg tgatgacccc ttccgggatt accgcctgac
     4561 gccggtttcg ccagttcgcg agttcgtaga ccagggtctt gggaacgtct ttgatcacgg
     4621 cgaaacctcc cgccatgtcg ccgtagagcg tcgcatagcc gacgctcatc tcactcttgt
     4681 tgccggtggt aaggacgagc cacccgaact tgttggacag cgccatcagg agcgtgcccc
     4741 gaatgcgcgc ctgcacgttc tcctcggtca cgtctggagg ccggcctgcg ctcaccggct
     4801 gtagggcggc gaggaaggct tggaagattc cgtcgatacc catgtcgatc atctgtacgt
     4861 ccagattctg cgcgacctca aacgcgtagc gcccactctc cgcggaggtg aaccgcgccg
     4921 gcaagaacgc cgtgtgaaca tgctcattgc caagcgcgtc gcacgcgatc gtggccacca
     4981 aggcggagtc gatgccaccg gagagcccga tcacgacgtc tctaaatccg ttcttccgga
     5041 cataatctcg cgtgcctagg accagcgccc ggtacacttc ctccgagggc tcatccggcg
     5101 gttcgagcgt ggctttgcgc ggagtcttgg gccttgcggg tgcatccgaa atgtggatcg
     5161 tgaacgattc gatgtcgcga tcctcaccct cgacaggcgg aaccggagaa aacatgcgat
     5221 gacggcgcac cgcttcaggg tcgatgtcac tgacgatgaa atcctcatcg aactgccggc
     5281 cgcgggccag cagcgtcccg gtgtgatcga aaatcatgcc catgccgtcg aagaccagtt
     5341 catcctgacc gccgaccaga ttgttgtaac agaggacgac ccggtagtct cgggcgcggg
     5401 tgccgagcat ctccgcccgc ccgcgccatt tgccggcatg gtaaggtgag ccgttgatgt
     5461 tgatgatcac cagcgcaccc tcagccgcct cggcctggca ggtcccgccg ggagcccaga
     5521 tatcctcgca gatggtgacc ccgacaggca ccccgttgat gacgtagacg ccggcgcgtc
     5581 ggccgggata gaagtacctt ttctcgtcga agacgccata gttaggcagt cgttgcttgt
     5641 ggtaaacgcc gacccgctcc ccgtccacgc aaatggcggc ggcgttgtag aggtgggcgt
     5701 cctggtcgac aaaaccgacc acggccgcga tcccatgcgt gcggcgggtg atttccccga
     5761 ctgccgccag gttgccgcgg acgaagtcag gcttcagcag caggtcttca ggtggatagc
     5821 cggggacgga gagctcagga aagcaaacga gatccacgcc gaggtctttg gcgtggtcga
     5881 tggcttcgca gattcgccgg gtattcccct ccagatcccc gacgacaagg ttcagttgcg
     5941 ccattccgac gcggagcatg gagcacccga aacttaatta tactgggaac aagggaacgg
     6001 gggaacgcgg gaacccgctg ggcgagcaga aatgccagcg gatacgatcg ctgagttagg
     6061 ccgggttgct ggagttaccg tacaacgccg gagcgttgca ggtaagtcca gtacctcctc
     6121 ccatctcgac gaaccgtatc cggctggcga gcatgttttt atcatgctgg tctcaaaggg
     6181 tcaatgcgga actgtcgatc gcgtgttgtg gatatcgggg acaacgtgga caacagacgc
     6241 aagcgcgcaa acgcgcaagc gcgaaaattc tacggcgaag gccgcgtgtt ttctgtcgat
     6301 atgatctgct gagcgatttc tttcatcggc cggcgggtgt tgcgactctc gcgttggatg
     6361 cgggtgaagg cttcggcctc ctggattccg agttttcgca tcagcacgcc cttcgcgcgt
     6421 tcgacgagtt tgcgagtttc gagagagttt tcgaggtcct cgacctcgcc gcgcagagcc
     6481 cgaatatccc gaaaccgcga cattgcgacc tcaatggccg gccggagatc gctttcccgc
     6541 acgggcttca ccaggtatgc caggaccccc gcgcccttgg ctgaatccac aaactcgcgg
     6601 tcgctgtagg ccgtgaggat caccacggga gtgttcccat ctcgctgcag gcgccttgcg
     6661 gcgtgcacgc catcgacccc cggcatcttg atgtcgagga agacgagatc gggtttgagc
     6721 ctggacacga ggtccagcgc ccgtgatcca tccgcggctt cgcccaccac ccgataccct
     6781 tgggcctcga gcatggtgcg aaggcccagc ctgatcacgg cctcatcgtc ggcgacaagt
     6841 attcttattg ctttagtgga gttttgtggc atgtggctgc ctactgttta ctgcctactg
     6901 cctaccagcg ccggaggcgc tgatatttgg gatcgtaatc gtcactgtcg ttccggtctt
     6961 gttggtgaat gtcaggctcc cacccagatc ttcggccacg agagcgtgga cgatcttgag
     7021 cccgagatca tcgccggcca tcgggtcaaa attggcgggc agcccggcgc cattgtcctg
     7081 tacctggagc accaacttgc cctcgcgctg ggcgagacgg atctcgacgt gtccatccgc
     7141 ccggtcagcg aacgcatgtt caacggcgtt ctgaagcagc tcgttgaccg cgagcgcaag
     7201 cgaggtggcc tgctgtgagt tgaggtagat gtcgtcgccc gtgaccgata cggtcagggc
     7261 tcgacccggc tgcgccatcg tctgcgtgac gttttgcgca accctgtcca gcagctgtct
     7321 gaggttgatg aggcggaagc cctcgaccga aagaatctcg tgcaccgccg cgatgctgag
     7381 cacccggtta atggtttcac tcaagacctc acggcctgaa acctcgcggc catcccggag
     7441 ctgcagtctc aggagcatgg cgacggtttg aaggttgttc ttgacccggt gatgcatctc
     7501 gcgaatgatc gccgaccgca ccacgaggtt ggcgttctca attgcgaggg cggtctggtt
     7561 ggccagtgtc gtgagcagct tgacttccgc atccgtgaac cgatacggtc gatccttgta
     7621 gcagttgaac accccgatca ccttgtctcg gacaacgaga ggcaccgaga gcagggcccg
     7681 caggccctcc tgctgtgcga cttctttggt tacgaatcgc ggttcggtga gcacatcggt
     7741 caccgcgata ggcttaccgt cttttgcgac caaacccgcg atgccctcgc caaccttcag
     7801 gtggcggcgt ttcagatacg ccgcgccggc actctgagcg gcggcgatga cgagctcgcc
     7861 gctgtcctcg tcgatcaaca tcaatgaggc cgtcttcgcc gtgagcatcc gcgcggccat
     7921 ctcgacgatg aggcgcagaa tttcctcgag gtaaaggggc gacgtgatcg tttcactgac
     7981 ttcggccagt gtggatagct ccataatctg ccggcgcatg ttatcgtaca gcgtcgcctt
     8041 ctcgatggcg ccggaggcga gatcggcgat cacgccgagc agttccatct cgttgttcga
     8101 atactcgtgg cgggcagtgg tctgtacgtt gacggcgccg agcacccggc cggcggtgac
     8161 aagcggcacc gcggccaggg agcggaaggt agattcgcgg gtttccggca gatacacgaa
     8221 acgaggatcg cgtgcggcat cgctgctcgc gacagcctct ccctcctttg cagcccaacc
     8281 ggtgaggccc tcgccgaagc gcaggcgcgc gcgtccgacc gcgccctccg cgaggcccgt
     8341 ggtcgccttc aacacgagat actctcctga ggggtcaagc aggtaaatcg agcagctgtc
     8401 catgcccatg acttcagccg tagttttggt gataaggtga agggtggtgt ccagatccag
     8461 cgcttcccca atcgcgcggc tgatggcgcg gagggctgaa atctctgctg ttttctggct
     8521 ggtcattggc ttggcgacat aataacaggt tcgtgtgcgt gctttcgggt ggcggcctgc
     8581 ttttggggag gtcgcaacct ctcccgactg aaacggatac ggggaccctc aggctacgat
     8641 ctccccggcg caggccaatg tctaatggca acggataccc tccgcagtca acttgttgag
     8701 caactaaagc gcagccggtc gatcacatcg cccgctgtcg aagccgcgtt tcgaagagtc
     8761 c
//
LOCUS       DBNGSB010000192         5084 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2745616, whole genome shotgun sequence.
ACCESSION   DBNGSB010000192 DBNGSB010000000
VERSION     DBNGSB010000192.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 5084)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5084)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5084
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2745616"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1046)
                     /gene="glnA"
                     /locus_tag="AC159B_05535"
     CDS             complement(<1..1046)
                     /gene="glnA"
                     /locus_tag="AC159B_05535"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HIR3377240.1"
                     /translation="MSTKVRNGLPETRTTPKEVIELCRRQEIKMVDYRFADLVGTWQH
                     FSSPVEELEESIFVDGIGFDGSSIRGFQRIDESDMLLIPDANTASVDPLLRVPTMLLT
                     CDIFDPVTRARYTRDPRYVAQKAEAYLVKTGIATTSYWGPEAEFFVFDDVRYDQSPHA
                     AFYSVDASEGIWNSGRDEKPNLGHKPRHKEGYFPVPPADSLQDFRSGLVLKMLEAGLE
                     VEVHHHEVASAGQCEIDLRFTTLTDMSDGMMAYKYLVKMYAREHFKTATFMPKPLFGD
                     NGSGMHCHQSLWKDGTNLFHDPDGYAQTSEMARFYIGGLLAHSPALMAFCAPTTNSYR
                     RLVPGFEAPVNLVYS"
     gene            complement(1059..2357)
                     /locus_tag="AC159B_05540"
     CDS             complement(1059..2357)
                     /locus_tag="AC159B_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007958689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIR3377241.1"
                     /translation="MAGSINAGDTAWLLTSTLLVMLMTPGLALFYGGLVRGKNVVSTL
                     MQSFFILALISVQWVLWGYTLAFGPDRGGLIGGLEWFALLGVGGEPNKDYAATIPHLA
                     FMAFQMMFAIITPALITGAFAERKRFTAFALFSLLWATLVYDPIAHWVWGMGGWLRTL
                     GAIDFAGGTVVHISSGVSALVAALVLGPRIKSPDNGALEGHNLTITALGAALLWFGWF
                     GFNAGSALTSGALAATAFVATNSAAAMAGLTWMIIGWKHQGRPSLVGLCTGSVAGLVA
                     VTPAAGYVNVAGALVIGMVAGALCYAAVHFTKQRLRVDDALDVFGVHGIGGLWGALAT
                     GIFATTAVNSAGANGLLAGNPMQLVYQAIAAVTTFAYAGGMTWLILKLLDLTIGIRVP
                     EREEVLGLDFTQHGEMAYRLGLTSEMNGLESSIVTSASQR"
     gene            2517..3695
                     /locus_tag="AC159B_05545"
     CDS             2517..3695
                     /locus_tag="AC159B_05545"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006889802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3377242.1"
                     /translation="MATYKYLIIGGGMAGDSALKGIREVDATATVGLIGAESQPPYNR
                     PPLTKGLWKGEPEEKIWRQTDSLGADLHLGRRAEQLDARNRQVTDDRGTTYGYEKLLL
                     ATGGTPKRLPGDPEGVIYYRTYRDYRTLRERAEDGERFTVVGSGFIGSEIAAALAMNG
                     KHVTMIFRGDGVGDNLFPRGLVKFLNDYFSGKGVELVTADEVASSSRKGATYSIGTKG
                     GRKIEADGIIAGVGITPNTQLAERAGLKVENGIIVDELLRTSRPEIYAAGDVANFYNP
                     ALGARLRVEHEDNANDMGEAAGKNMAGETKPYHHLPFFYSDLFDLGYEAVGDVNAKLP
                     AVEDWKEPYKEGVVYYLKDGRVKGVLLWNVWEQVDNARKLIAEKGPFKPEDLKGRLPA
                     "
     gene            complement(3702..4484)
                     /locus_tag="AC159B_05550"
     CDS             complement(3702..4484)
                     /locus_tag="AC159B_05550"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3377243.1"
                     /translation="MKIVRDGVEISYKDEGEGLPVVLLHGFPFSGRMWTRQVAALASA
                     HRVITPDFRGFGESGGVPSTVEQLAGDVEALADHLELEPFVLGGFSMGGYVAFRYLAQ
                     HTDRVHALMLLDTRAEADAPEAKQRRYAGIDRIKSEGPDGFLNDFAKLVLSPKTVESR
                     PEIVTQVRQMMEEERAASLTGGLKAIAERPDSTPLLGRLKMRTLIVVGEDDKATPPES
                     AKKMQGAIPGSKLVTIPGAGHMTSIEQPERFTKALREFLEAV"
     gene            complement(4459..5052)
                     /locus_tag="AC159B_05555"
     CDS             complement(4459..5052)
                     /locus_tag="AC159B_05555"
                     /inference="COORDINATES: protein motif:HMM:NF013921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR3377244.1"
                     /translation="MPRRPRGLIVQAFVHVVATGNRGFPLYLHQRDFESFLGRLEQYA
                     AAGSARVHAYCLMTNHLHLLLEAGQSPISRCMQSLLARYAEYVNRRYQYRGHLFGGRF
                     WSKVCLSDEYFLAVLRYIHRNPVRAGLVTNPEEYPWSSHRVYLGTLSVPWVTTKCLEL
                     FSLDKHRAIERYARFVSTGGEAHRQEGSADEDRPRWG"
BASE COUNT         1023 a         1637 c         1510 g          914 t
ORIGIN      
        1 gagtacacga ggttcaccgg cgcttcgaat cccgggacga ggcggcgata cgaattggtc
       61 gtcggggcgc agaacgccat gagcgcgggc gagtgcgcca gcaaccctcc gatgtagaat
      121 cgcgccatct cgctggtctg cgcgtaccca tcggggtcgt ggaacaggtt tgtgccgtcc
      181 ttccacaagc tctgatgaca gtgcatccct gatccgttgt cgccgaagag cggcttgggc
      241 atgaacgtgg ccgtcttgaa gtgctcgcgc gcgtacatct tcacaaggta tttgtacgcc
      301 atcatcccgt cgctcatgtc ggtgagcgtc gtgaagcgga ggtcgatctc gcactggccc
      361 gcgctcgcga cctcgtgatg gtgcacctcc acttccaacc ccgcttccag catcttgagc
      421 acgagacccg agcgaaagtc ctgcaggctg tcggccggcg gcaccgggaa gtagccctct
      481 ttgtggcgcg gtttgtgccc gaggttaggt ttttcatccc ggccgctgtt ccagatacct
      541 tcggaggcgt ccacgctgta gaatgcggcg tgcggactct ggtcgtagcg gacgtcgtcg
      601 aacacgaaga attcggcctc cggcccccag tagctggtgg tggcgatgcc ggtcttcacg
      661 aggtacgcct cagccttttg cgccacatag cggggatcgc gagtgtatcg cgcgcgcgtc
      721 accggatcaa agatgtcgca ggtcaaaagc atcgtcggga cccgcagcag cgggtcgacg
      781 ctggcagtat tggcatcggg gatcaggagc atgtcggatt cgtcgatccg ctggaacccg
      841 cggatgctgc tcccgtcgaa gccgatgcca tcgacgaaaa tactctcctc cagttcctcc
      901 accggcgagg agaagtgctg ccaggtgccc acgagatcgg caaagcgata atccaccatc
      961 ttgatttcct ggcgccggca gagttcaatc acttctttgg gcgtggtgcg cgtctccggc
     1021 aacccattgc ggaccttcgt actcatttcg cttcctcctc agcgctgaga tgcggacgtg
     1081 acgattgacg actcgagacc gttcatctct gaagtcagac ccagccggta tgccatctcg
     1141 ccgtgctgcg tgaagtccag gcccaggact tcttcgcgct cgggcacgcg aatcccgatc
     1201 gtcaagtcga gcagcttgag gatgagccac gtcatacctc cggcataggc aaacgtggtg
     1261 accgccgcaa tagcctggta gacgagctgc ataggattcc cggcgagcag gccattggcg
     1321 ccggcggaat tcaccgcggt cgtcgcaaag atccccgtcg ccagcgcgcc ccacaggcct
     1381 ccgatgccat gcacgccgaa aacgtcgagc gcgtcatcga cccgcagccg ctgcttcgtg
     1441 aagtgcaccg cggcataaca cagcgcgccc gcgaccatgc cgatcaccag cgcgcccgcg
     1501 acgttcacgt atcctgccgc gggcgtcacc gcgaccagcc ccgccacact gcccgtgcac
     1561 aggccgacca ggctggggcg accctgatgt ttccatccga tgatcatcca ggtcagtccg
     1621 gccatggctg cagccgagtt cgtcgccaca aacgccgtgg ccgccagagc gcccgacgtc
     1681 agcgcgcttc cggcgttgaa cccaaaccag ccgaaccaca acagcgcggc gcccagcgcg
     1741 gtaatggtca ggttgtgtcc ttcgagcgcg ccgttatcgg gtgatttgat ccgaggtccg
     1801 agcaccaacg ccgcgaccaa cgcggagacg cccgatgaga tatggacgac ggtgccgccg
     1861 gcgaagtcga tcgccccaag tgtgcgcagc cagcctccca tgccccacac ccagtgcgcg
     1921 atcggatcgt agacgagcgt cgcccagagc aggctgaaga gcgcgaaggc ggtgaaccgt
     1981 ttgcgctcgg cgaaggcgcc ggtgatcagg gccggcgtaa tgatcgcgaa catcatctga
     2041 aacgccatga acgcgaggtg ggggatggtg gccgcgtaat ccttgttggg ctctccgccc
     2101 acgccgagca gcgcaaacca ttccaatcct ccgatcaatc cgccgcgatc tgggccaaac
     2161 gcgagggtgt agccccacag cacccattgc acgctgatca atgccaggat gaagaagctc
     2221 tgcatcagcg tcgagacgac gttcttcccg cgcaccaggc ctccgtagaa gagcgccagc
     2281 cccggcgtca tcaacatcac caggagcgtg gaggtcaaca gccacgccgt gtcaccggca
     2341 ttgatcgaac ccgccatcgt ctcaccgcct ttgctataaa cagaaaacgg caccctccaa
     2401 agggagggcg ccgtggccca cagtgctgcc tgtgctcttg gcgagcacta tagcaaagtg
     2461 tcccccgggc cgtcaagagg aagtcgccct gccttagtga aggccaaaca tagcccatgg
     2521 cgacctacaa gtacctgatc atcggtgggg ggatggccgg tgactccgcc cttaaaggca
     2581 tcagggaagt cgatgccacc gcgaccgtcg gattgatcgg cgccgagtcg cagccgccct
     2641 acaaccggcc cccgctcacc aagggactat ggaaaggtga gcccgaggag aagatctggc
     2701 gccagaccga cagcctcggc gcggacctcc atctcggccg gcgggccgag cagctcgacg
     2761 cgcggaaccg tcaggtaacc gatgaccgcg ggaccacgta tggctatgaa aagctgctgc
     2821 tcgcaaccgg ggggacgccg aagcgcctgc caggcgatcc ggaaggggtc atctactatc
     2881 ggacataccg cgactatcgc accctgcgcg agcgcgcgga ggacggggaa cgcttcactg
     2941 tggttgggag cggcttcatc gggtcggaga tcgccgcggc tctggccatg aacggcaagc
     3001 acgtgacgat gatcttccga ggcgacggcg tgggcgataa tctgttccct cggggtcttg
     3061 tgaaattcct caacgactac ttctcgggga aaggcgtcga acttgtgacc gccgacgaag
     3121 tagcgagctc ctcacgcaaa ggtgcgacgt acagcatcgg gacgaaaggc ggccgcaaaa
     3181 tagaggccga tggcatcatc gccggcgtcg gcatcacacc caatacgcag ctggccgaac
     3241 gcgccggcct caaagtggaa aacggcatca tcgtcgacga gctcttgcgg acgagccggc
     3301 ccgagattta cgccgcgggc gatgtggcga acttttacaa ccctgcgctg ggcgcgcgtc
     3361 tgcgtgtcga gcacgaggac aatgcgaacg acatgggcga ggccgcaggt aagaacatgg
     3421 ccggcgaaac caagccctac caccacctgc cgttcttcta ttcggacttg tttgatctcg
     3481 gctacgaggc tgtgggcgac gtgaacgcaa agctccccgc agtcgaggat tggaaggaac
     3541 cgtataagga aggcgtcgtc tactatctca aagatggacg cgtcaagggt gtcctgctct
     3601 ggaacgtttg ggagcaggtc gacaatgcca gaaaactcat cgcagagaaa ggtcccttca
     3661 aacctgaaga tctgaagggc cggctccccg cctgatccgt cctagacggc ctcgagaaat
     3721 tctcgcaacg ctttggtgaa tcgctccggt tgctcgatac tcgtcatgtg tcctgccccg
     3781 gggatcgtga ccagcttcga cccgggaatc gccccctgca ttttcttcgc gctctccggg
     3841 ggcgtcgctt tatcgtcctc gcccacaaca atcaacgtcc gcattttcag tcggcccagc
     3901 agcggcgtcg aatcaggccg ctccgcgatc gccttcaacc caccggtcag cgatgctgct
     3961 cgctcctcct ccatcatctg tcgtacttga gtgacaatct ctggacgcga ctccacggtt
     4021 ttcggcgaga ggacaagctt cgcgaagtca ttcaggaatc cgtccggtcc ttctgatttg
     4081 atccgatcga ttccggcata gcgccgctgc ttggcctccg gcgcgtccgc ctccgcgcgc
     4141 gtatccagca gcatcagggc gtgtacccta tccgtgtgtt gcgccaggta ccgaaacgcc
     4201 acgtagcccc ccattgagaa gcccccgagc acgaacggct cgagctccag gtgatcagcg
     4261 agcgcctcga cgtctcccgc cagctgctcc acagtcgagg gcacgccgcc ggattcccca
     4321 aatccccgga aatcgggcgt aatcacgcga tgtgcagacg caagggcggc gacttgccgg
     4381 gtccacatcc gtcctgagaa tgggaacccg tgcaggagca cgaccggcaa cccctcgcct
     4441 tcatccttat agctaatctc aaccccatct cggacgatct tcatcggctg atccctcctg
     4501 tctatgtgct tcgccaccgg tgctcacaaa ccgtgcgtat cgttcaatcg cacgatgctt
     4561 atccaggcta aacagctcca ggcacttcgt ggtcacccat ggaacgctca gcgtcccgag
     4621 gtaaacacgg tggctgctcc agggatactc ttctggattc gtgacaagcc ccgcccgaac
     4681 tgggttgcga tgaatgtacc gcagcacggc aaggaaatat tcatcgctca agcatacctt
     4741 cgaccagaat cgaccgccaa ataggtgccc gcggtactgg taccggcggt tcacatactc
     4801 cgcgtatcga gcgagcaggg attgcatgca tcttgagatc ggcgattggc ccgcctccaa
     4861 caggaggtga agatgattgg tcattaggca gtaggcatga actcttgctg aaccggctgc
     4921 cgcatactgt tccaggcgac ccaggaatga ctcaaagtct cgctggtgga ggtagagggg
     4981 gaaaccacga ttccctgtgg ccaccacatg aacgaatgct tggactatga ggccgcgagg
     5041 ccgtctgggc actgtcccac cccgtcgttc ccgagggtag cagc
//
LOCUS       DBNGSB010000193        12546 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2901502, whole genome shotgun sequence.
ACCESSION   DBNGSB010000193 DBNGSB010000000
VERSION     DBNGSB010000193.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 12546)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12546)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12546
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2901502"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..730)
                     /locus_tag="AC159B_05560"
     CDS             complement(119..730)
                     /locus_tag="AC159B_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3377245.1"
                     /translation="MKVNRREFLHRVGAGSIALASLPALMDMFKLPALAQSERGFLFL
                     CVNAAGTPPAHLMGIGGVARYDPSKGPGSPVTGGGSFFHWTPSATAPAPLVASGTWKT
                     RLLTSYREMATYGGVAAGVGEFVVDIQRLRPSPALIRGAALTVYCTLGPAGITAAQTG
                     GIEGVTLSIPGTEFSAGGNPGIFRQIPPGGTGVTIFLLPESLT"
     gene            complement(858..1979)
                     /locus_tag="AC159B_05565"
     CDS             complement(858..1979)
                     /locus_tag="AC159B_05565"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR3377246.1"
                     /translation="MRGAIRLGRILGIPIGVNYTWFIALWLLTWSLARTYYPSRFPGY
                     DGQIYLLMGLASALLLFASVLVHELGHAVAARRYGIRTRSIVLFLFGGVAQIGKEPPT
                     PASELVVAATGPATSLLLGGFCWVVSRLATGTALGAIIAYLAWVNAALAIFNLIPGFP
                     LDGGRMLRALLWRAMGSLERATRIAARGGQIVALLLIALGVLFVFTGNVTNGVWFVLI
                     GWFLDMGAQAGYQQVLLRETLGGVRVGDIMSRKIHSVDQNLTLEQLIADYFLPYKHGG
                     FPVMWGDRLVGIITLHDVKEVPREKRATMTVRDAMTPTSRLRTVKPTASAYDAFARMA
                     QDGIGRLLVVDDSGELVGILTRSDLLHVLRIRTELEELA"
     gene            complement(2016..3362)
                     /gene="chrA"
                     /locus_tag="AC159B_05570"
     CDS             complement(2016..3362)
                     /gene="chrA"
                     /locus_tag="AC159B_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HIR3377247.1"
                     /translation="MSHPVGFWEAFRFWLKLGLINFGGPTGQIALMHQELVDRRRWIS
                     NDRFLHSLNYCMLLPGPEATQLAIYVGWLLHRTAGGMVAGVLFVLPAYFVLLLLSWTY
                     AVHGRVPWVAMIFYGLRAAVIAIVAAAVIRIGSRALRNRIMAAIAALTFVAIFFFKAP
                     FPAIVISAGLLGLIGSRYRPEIFAVSEPHTPGAPPTAIADHGPLPSHTRVNARRLLTV
                     LVIGLLLWWVPLLVVIFWRGPGDVLAQEAVFFSKAALITFGGAYAVLAYLTQAAVEHF
                     GWLAPGEMLDGLGLAESTPGPLIMVTEFVGFLGAFRHPAELEPVLAGVLGASISIWAT
                     FAPSFLFIFLGAPFIERLRGNVPLGGALSTITASVVGVILNLAVWFGLHTMFGAVTER
                     RVLGITVPVVDIRSLDVVALLIAVVAFVGMWRFRWSFVPVVLGSGLAGFLVKTLLR"
     gene            3558..3896
                     /gene="trxA"
                     /locus_tag="AC159B_05575"
     CDS             3558..3896
                     /gene="trxA"
                     /locus_tag="AC159B_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR3377248.1"
                     /translation="MGKATHVNEQEFESQVLKAPTPVLVDFWAEWCGPCRMIAPIVEE
                     LAGEYEGKMKVVKVDVDSNQDIAFKYGIMSIPTLGIFKGGEMVERLVGYMPKQQLKRR
                     IDTALSATVS"
     gene            complement(4028..4957)
                     /gene="trxB"
                     /locus_tag="AC159B_05580"
     CDS             complement(4028..4957)
                     /gene="trxB"
                     /locus_tag="AC159B_05580"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HIR3377249.1"
                     /translation="MDGKVRNVIILGGGPAGLTAAIYATRAGLTPLIIEGSEAGGQLM
                     LTTDVENYPGFAEPILGPDLMAAIRAQALRIGTEFVTEDATAVDLSTRPFKVTTHSGE
                     TYETRALIIATGAKAKLLGLPGEQRLMGRGVSTCATCDGFFFRGSDVMVVGGGDTAME
                     EALYLSKLAKSVTVVHRRDRLRASKIMQERAMQNGKISFLWNTKVADIVGDGKVSGVK
                     LQDLKTHTTTQRPVDGVFVAIGHTPNTDLVKGQLEMDANGYIARKHRSMTNVDGVFVA
                     GDVHDHTYRQAVTAAGYGSEAAIDAERWLATQS"
     gene            5094..5411
                     /locus_tag="AC159B_05585"
     CDS             5094..5411
                     /locus_tag="AC159B_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014516112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR3377250.1"
                     /translation="MRPGPRRGNAPVYVISIAAELTGLHPRTLRIYEEKGLVQPARRN
                     NNRLYSEHDLDRVRLIQHLTQEVGLNLAGVKVLLEVYERLEYRKSKDAISWVFERSVR
                     RRR"
     gene            5408..5857
                     /locus_tag="AC159B_05590"
     CDS             5408..5857
                     /locus_tag="AC159B_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR3377251.1"
                     /translation="MKMNLIRWDPFDDLASLRESMDKLFEEFVTRRPVRGATIAVWQP
                     AVEIFETDSEVVVRVELPGVDPKTVDITVTEDALTIKGEHRIEQESKGRNYFRRELRY
                     GAFLRSLALPAGVQGDQAKAAFKNGILEITVPKSERAKPKAVKVEIG"
     gene            5875..7791
                     /gene="dnaK"
                     /locus_tag="AC159B_05595"
     CDS             5875..7791
                     /gene="dnaK"
                     /locus_tag="AC159B_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIR3377252.1"
                     /translation="MPKVVGIDLGTTNSVIAVVIGGEPQVIPNAEGSRLTPSVVAFSK
                     TGERLVGQMAKRQAILNPENTVYSIKRFMGRRLAEVEAERKMVPYKVEEGSNGMVTVR
                     LPAAGKTFTPEEISAMILQKLKTDAEAYLGDKVDEAVITVPAYFNDAQRTATKNAGEI
                     AGLKVLRIINEPTASALAYGMDKKVAETVLVFDLGGGTFDVTVLEIGEGVFEVKATNG
                     DTHLGGDDWDERVVHWLADQFKRENGIDLRADRQALQRLREASERAKVELSTVTQTTI
                     NLPFITADATGPKHLDYMLTRAKFEEMTADLVERCIGPFKQALSDAKMTEKDLDQVIL
                     VGGATRMPMIQELVRRLTGKEPNKEVHPDEVVAVGAAIQAGVLAGTVREVVLLDVTPL
                     SLGVETLGGVMTPLIARNTTIPTRKSEVFSTAADGQTQVEINVQQGERPMARDNRTLG
                     RFILDGIPPAPRGIPKIEVTFDIDANGILDVKAKDTATGREQSIKITGTSTLSKDEVE
                     KMVKESERFAEEDKRKRDAAEARNRGDSMVYQTERMLKEAGDKVSSDERQSLETKLTA
                     LKEALGSDDVERINAAINELQQASYKLAEAMYAKTAASGGGASPGPESQGPAGEGKSE
                     DVIDADYKKTDEKR"
     gene            7792..8361
                     /locus_tag="AC159B_05600"
     CDS             7792..8361
                     /locus_tag="AC159B_05600"
                     /inference="COORDINATES: protein motif:HMM:NF013212.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIR3377253.1"
                     /translation="MTESTTRWSMDEREDRPKNEGLETSGTLSDATETVEELLAKVKH
                     YKEEAERNWQQFLHAAADLENYKKQAARQRDDAVQRVRTSLLNVILTVVDNLDRALEH
                     ADKASRAENILEGIRMTHRQVIDLLQSLGVQSIDSVGKPFDPKMHEAVDVASSRDHSV
                     DPGTVVEELQKGYLFNGEVLRPARVRVAN"
     gene            8364..9353
                     /locus_tag="AC159B_05605"
     CDS             8364..9353
                     /locus_tag="AC159B_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HIR3377254.1"
                     /translation="MEFKDYYKTLGVERSADQKEISKTYRRLARQFHPDVNKNKGAED
                     RFKEINEAYQVLGKPDQRAKYDQLYEAYQHGGMDWQQIFGRGGGYQTPEGWTVTFGGD
                     AENLEDVLGGFSDFFKQFFGQEFATTRTARSPFGRRQTVDRAYSRRAGVEELMSEQEP
                     AAAESMLDITLEEAFRGSQKPVALQLNGTTRRLDVTIPKGVRDRQKIRLSGALDGRDL
                     YLTIRVLPHPQFERRDDDIVVEVPVTLSEAMLGAKIRVPTLEGTVDMTIPPETQNGQM
                     FRFREQGMPRRDGGRGDQMVRVKVVLPTKLSARERQLFEELRTLRKEDSREAR"
     gene            9361..11985
                     /gene="clpB"
                     /locus_tag="AC159B_05610"
     CDS             9361..11985
                     /gene="clpB"
                     /locus_tag="AC159B_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HIR3377255.1"
                     /translation="MRFDKFTEKAQEAILHAQETMQKFGHPQVEPEHLLLALTEQAEG
                     VVPAIISRAGANAAALQGRLRNELGRRPKVQAPQQAEGLYLSSALRQAIETAQQEAQR
                     MRDEYVSTEHLLLGIAQDRDGLAGRLLAEAGLTQDAIYRVLQEVRGSQRVTDQNPESK
                     YQALERYGRDLTQQAAAGKLDPVIGRDEEIRRVIQVLSRRTKNNPVLIGDPGVGKTAI
                     VEGLAQRIVRGDVPEGLKHKRVVQLDMGALVAGTKFRGEFEERLKAVLKEIADAAGEI
                     ILFIDELHTVVGAGAAEGAIDAANMLKPTLARGELRTIGATTLDEYRKHIEKDAALER
                     RFQPVLVGEPSVEETISILRGLKERYEVHHGVRITDAAVIAASTLSDRYISDRFLPDQ
                     AIDLIDEAASRLRMEIDSKPVELDEIDRRIMQLEIERQALRKERDPGSQERLQRLEQE
                     VAKLKAESEQLRKQWEEEKRAISAIRETKERIEETRQQIEEAERRADLEAAARLRYGT
                     LRELQERMASQEQALKDIHRGRHLLKQEVDPDDVAQVVAKWTGIPVTRLLEGEMQKLL
                     TMEQRLHERVVGQNEAVAAVSDAIRRARAGLKDPRRPIGSFLFLGPTGVGKTELARAL
                     AQHMFDDEEAMIRLDMSEYQEKHTVSRLIGAPPGYVGFDEGGQLTEAVRRRPYRVVLF
                     DEIEKAHTDVFNILLQILEDGRLTDGHGRTVDFKNTVIIMTSNLGSRFLQHLDARDAA
                     QFEMARVQVLDEVRKAFRPEFLNRIDEIIIFHPLSAEQLRQIVDLQLRYLHDRLASQK
                     MGLEVIDAAKDYLAREGYNPDFGARPLRRLIQREVENRIARMVLAAELREGFAAVVDY
                     KHGEIKITIRERVAVTSK"
     gene            12022..>12546
                     /locus_tag="AC159B_05615"
     CDS             12022..>12546
                     /locus_tag="AC159B_05615"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HIR3377256.1"
                     /translation="MSGAVLLALEIVASRIIAPFFGNSIYVWGSLIGVFLAALALGYF
                     LGGQAADRWPSLAVLAGCVFLAGALVVPIPVVAPRVLEVIILRDPGPRISPLVGSLAL
                     FFLPGVVIGMVSPFAVRLRARTVDTIGNVAGALYALSTLGSIAGTLLASFVLIAVTDV
                     SQIVYALGAALLVLA"
BASE COUNT         2636 a         3742 c         4045 g         2123 t
ORIGIN      
        1 ttccaccggc gatgtcccgg cgagcagcaa ctccagcatc acaggcagga atttcatcgg
       61 tcaactcccg ccgagctaag aatccttgtg gccttcccct caaagccaat cgcgaatgct
      121 acgtcagtga ttcaggcaac aggaaaatgg tgacccccgt cccccctggc gggatctggc
      181 ggaaaatacc tgggtttcct cccgcagaga actccgtccc tggaatgctg agggtaacgc
      241 cttcaatccc accggtttgg gctgcagtga ttccggcggg tccgagggtg cagtagacag
      301 taagcgcggc gccgcggatc aacgctggag atggccgcag ccgctggatg tcgaccacga
      361 actcaccgac ccctgccgca acaccgccgt aagtggccat ctccctatag ctcgtgagta
      421 gtctggtctt ccatgtgcct gatgccacaa gaggagccgg ggcggtcgcc gagggcgtcc
      481 agtggaagaa gcttcccccg cctgtgaccg gggagccagg acccttagaa ggatcgtacc
      541 gggctactcc gcctatgccc atcaggtgtg ccggcggggt gccggcggcg tttacgcaga
      601 ggaatagaaa acctcgctcc gactgggcca gcgcaggcag cttgaacata tccatcagag
      661 ccggcagcga ggcgagcgcg atcgagcctg ctcctaccct gtgcaagaac tccctgcgat
      721 tgactttcat cgttgtcacc tccagggata atgagatcct tgcaatgcag agttcgttcg
      781 ctgcagcagt gaaaaggtga gagaacagca ggggttactt atgcagcctt tcgtacacaa
      841 ccaagcaggt cctgcttcta cgcgagttct tcgagttcgg tccggattcg cagcacatgc
      901 aagaggtcgc tgcgggtcaa gatccctacg agttcgccgc tgtcgtccac gaccagcagg
      961 cggccgatcc catcttgcgc catccgggcg aacgcgtcgt acgcggaagc cgttggtttc
     1021 acggtgcgca agcgcgaggt cggcgtcatc gcgtctcgga cggtcatggt cgcccgcttc
     1081 tctctcggca cttctttaac atcgtgcagg gtaatgatac cgacaaggcg gtcgccccac
     1141 atcactggga acccaccgtg cttgtagggg aggaagtagt cggcgatcaa ctgctcgagc
     1201 gtcaggtttt gatccacgga atgaatcttc cggctcatga tgtcgccgac gcgcacgccg
     1261 ccgagggtct cgcggagcag cacctgctga taacctgctt gggcccccat gtcgagaaac
     1321 cacccaatga ggacgaacca cactccgttg gtcacattgc ccgtgaagac gaacagcacg
     1381 ccaagcgcaa tgagcaggag cgccacgatc tgccctcccc gagcggcaat gcgggtcgcc
     1441 cgctcgaggc tgcccatcgc gcgccacagc agcgcgcgga gcatgcgccc accatccagc
     1501 ggaaagcccg ggatcaagtt gaagatggcg agtgcggcgt tgacccaggc caggtaagcg
     1561 ataatcgccc cgagcgcggt cccggtggcc aggcgtgata ccacccagca gaaaccgccg
     1621 agaagcaggc tggtggccgg tcctgtagcc gcaacgacga gttcgcttgc gggcgtcggc
     1681 ggttcctttc cgatctgcgc gactccgccg aataggaaga ggacaatgct gcgcgtgcgg
     1741 atcccatacc tccgcgccgc gaccgcgtga cccagctcgt ggacgaggac gctcgcgaac
     1801 aacaggagcg ccgaggccaa tcccattagc agatagatct gaccgtcata cccggggaag
     1861 cggctcggat aatacgtccg cgccagggac cacgtgagga gccacagcgc aataaaccac
     1921 gtgtagttga cgccgatagg aatgcccaga atgcggccca ggcggatggc accacgcatg
     1981 atactccttg atttcccccg gctcttccgc gagtcctagc gcaacaaagt cttcaccaga
     2041 aaacccgcga gtccactccc aaggacgacc ggcacaaaac tccagcgaaa ccgccacata
     2101 ccgacaaagg cgacgaccgc gatcagcaac gccaccacat ccagtgacct gatatcaacc
     2161 accggaacgg taatgccgag cacgcgccgt tctgtgactg cgccgaacat ggtgtgaagt
     2221 ccgaaccaca ccgcgaggtt gaggatgacc ccgacgacgc ttgcggtgat cgtcgacaac
     2281 gcgccgccca gcggcacgtt tcccctgagg cgttcgatga acggggcgcc gagaaagatg
     2341 aacaggaagg acggcgcgaa tgtcgcccag attgaaatcg aggctccgag cactcccgcc
     2401 agcaccggct ccagttcggc gggatggcgg aacgcgccga ggaatcccac gaactccgtg
     2461 accatgatga gcggcccggg ggtggactcg gcgagaccga gtccgtcgag catctcgccg
     2521 ggagcgagcc acccgaaatg ttcgaccgcc gcctgcgtca gataggccag cactgcgtac
     2581 gctccgccga acgtgatcaa cgccgccttg ctgaaaaaca ccgcctcttg cgccagcacg
     2641 tcaccgggtc cgcgccagaa gatgaccacc agtaggggaa cccaccacag cagcaggccg
     2701 atgaccagca ccgtgaggag ccgccgcgca tttactcgtg tgtgactggg gagcggcccg
     2761 tgatccgcga tggctgtcgg cggcgcgccc ggggtgtgag gctcagaaac ggcaaagatt
     2821 tccggccggt agcgcgatcc gatcaacccg agcagtcctg ctgagatgac gatggccgga
     2881 aacggcgcct taaagaagaa gatggcgaca aaggtcaggg ccgcgatcgc tgccataatg
     2941 cggttcctga gcgcccggct gccgatgcgt atcacggctg ccgcgacgat ggcgatgacc
     3001 gccgcccgca atccgtagaa gatcatcgca acccatggaa ccctcccgtg cactgcatag
     3061 gtccaggaca gcagcagcag cacgaaataa gcgggcagca caaagaggac gccggcgacc
     3121 atccccccgg ctgtgcggtg gaggagccat ccgacataaa tcgcaagctg cgtggcttcg
     3181 ggaccaggca gcagcatgca gtagttgaga gagtggagga accggtcgtt gctgatccag
     3241 cggcgccggt ccaccagttc ctgatgcatg agcgcgatct ggccggtggg accaccgaag
     3301 ttgatgagtc ccagcttcaa ccaaaaccgg aatgcttccc agaaaccgac tggatgactc
     3361 atctgggtca tcgacttctg tcttgcagag tacctacctt gaataggagc cggccccggg
     3421 tgccaggcac ctggacagga ggtgggcatt gccgtggccg ccgcgcaata tgtcctggga
     3481 gattcgtgcg agctggtgga ataatagaag agtgggcatc gttctgatag ttgcactcat
     3541 acgataggag cgtgcacatg ggcaaggcca ctcacgtcaa tgaacaagaa ttcgagagcc
     3601 aggtgctgaa agcacccacg ccggtgctag tggatttttg ggcggaatgg tgcggtccat
     3661 gccggatgat cgcgcccatc gtcgaggaac tggcgggcga gtatgagggc aagatgaagg
     3721 tcgtgaaggt ggacgtcgac agtaatcagg acattgcgtt caagtacggg atcatgagca
     3781 ttccgacgct tgggatcttc aagggcggcg agatggtcga gcgtctcgtc ggctacatgc
     3841 cgaagcagca gctcaagcgg cggatcgaca ccgcgctgag cgcgaccgtg agctgacaac
     3901 cgggaacggg gaaccgggaa cggcgaacgg accatagtag gtcacgggag ttcggggttt
     3961 accgagctcc cgtaagtcgt ttttggggag ttactgttcc ccgttgaccg ttccccgttc
     4021 ccctcagtta gctttgggtc gccagccagc gttcggcatc aatagccgct tccgacccat
     4081 atcccgcggc cgtcacggcc tggcggtaag tgtgatcgtg cacgtcaccc gcgacgaaga
     4141 cgccatcgac attggtcatg gagcggtgct ttcgtgcgat atagccgttc gcatccattt
     4201 cgagctgtcc cttaacgagg tcggtgtttg gcgtatggcc gattgcaaca aacacgccgt
     4261 ccacgggtcg ctgcgtggtc gtatgcgtct tcagatcctg taacttcaca ccgctcactt
     4321 tgccatcacc gacaatatca gcgacctttg tgttccacag gaacgagatc ttgccattct
     4381 gcattgcgcg ctcttgcatg atcttgctgg cgcgcagccg gtctctccgg tgaacgaccg
     4441 tcaccgattt cgcgagcttc gagaggtaga gcgcttcctc catcgcggtg tcgccgccgc
     4501 ccaccaccat gacgtcgctg ccgcggaaga agaagccgtc gcaagtcgcg caggtgctga
     4561 ccccccgccc catcagccgc tgctcgcccg gcagacctaa gagtttcgct ttcgcccccg
     4621 tcgcgatgat gagggctcgc gtttcatacg tttccccact atgggtcgtg accttgaagg
     4681 gccgcgttga gaggtcgacc gccgtggcgt cttcggtgac aaactccgtg ccgatccgca
     4741 gcgcttgcgc gcgtatcgcc gccatgagat ccggccccag gatgggttcg gcgaagccgg
     4801 ggtagttctc gacatcggtg gtcagcatga gctggccacc cgcttcggaa ccctcgatga
     4861 tgagcggggt caggcccgcg cgggtcgcgt agattgcggc cgtcaggcct gccggtccgc
     4921 cgccgagtat aatgacgttg cgcaccttac cgtccatatc acacctttaa cggaacggac
     4981 aacatcgata ttccttcgga gccccgcggc ggccttccta tgacaatacc attgtcaata
     5041 ttcttgacaa tgatggttcc cctaggtacc tttaagaggt ggagaagcat gccgtgagac
     5101 ccggacctcg tcgcggaaac gcgcctgtct acgtgatcag catcgccgcg gaactgacgg
     5161 ggttgcaccc gcggacgctg cgtatttatg aggaaaaagg actggtgcag ccggcgcggc
     5221 gtaacaacaa ccggctgtat tcggagcacg acctggaccg cgtgcggttg attcaacacc
     5281 tcacgcagga ggttgggctc aacctggccg gggtcaaagt gctgctcgag gtatatgagc
     5341 ggctggaata ccggaagtcg aaggacgcca tctcgtgggt gtttgaacgt tccgtccgga
     5401 ggaggagatg aagatgaatt tgatccgctg ggaccccttt gacgacctcg cctccctgcg
     5461 ggagtcgatg gacaagctgt tcgaggagtt tgtgacccgc cgccccgtgc ggggggcgac
     5521 gattgcggtc tggcagcctg cggtcgagat cttcgagacc gacagcgaag ttgtcgtccg
     5581 ggtcgaactg cccggcgtcg atcccaagac ggtcgatatc acagtgaccg aggacgcgct
     5641 caccatcaaa ggcgagcatc gaatcgagca ggagagcaaa ggccggaact acttccgacg
     5701 tgagctgcgg tacggtgctt tcctgcgctc tctggcgctg ccggcgggcg ttcaagggga
     5761 tcaggccaag gcggcattta agaacggcat cctcgaaatt acggtgccca agagcgagcg
     5821 cgcgaaacca aaggctgtca aagtcgagat cggctaggag aggagtttac tgctatgcca
     5881 aaagttgtcg gcattgatct ggggacgacc aactcggtca tcgcggtggt gatcggaggc
     5941 gagccccagg taattcccaa tgcggagggc agccggctca cgccctctgt cgtcgcattt
     6001 tcaaagaccg gtgagcgact cgttggccag atggccaaac gccaggcgat cctcaacccg
     6061 gagaacacgg tgtattccat caagcgcttc atgggccggc gtctcgcaga agtcgaggcc
     6121 gagcgcaaga tggtgccgta caaggtcgag gaaggcagca acggcatggt gacggtccgc
     6181 ctgcctgccg cgggcaagac gttcacgccc gaagagatct ccgcgatgat tctccagaaa
     6241 ctgaagaccg acgcggaagc atacctcggc gacaaggtcg acgaagccgt gatcacggtc
     6301 ccggcgtatt tcaacgacgc acagcggacc gcgaccaaga atgcgggcga gattgcgggc
     6361 ctcaaggtgc tccgcatcat caacgagccc accgcgtcgg ccctcgccta cgggatggat
     6421 aagaaggtcg ccgagactgt gctcgtcttt gatctgggcg gcggaacctt tgacgtgacg
     6481 gtgctggaga tcggcgaagg cgtcttcgaa gttaaggcga ccaatggcga cacgcacctg
     6541 ggcggcgacg actgggacga gcgcgtcgtg cactggcttg ccgatcaatt caagcgcgag
     6601 aacggcatcg acctccgcgc ggaccgtcag gcgctgcagc ggctgcgcga ggcatccgag
     6661 cgcgccaagg tcgagctcag tacggtgacc cagacgacca tcaacctgcc gtttatcacc
     6721 gccgacgcga cgggccccaa gcacctcgac tacatgctta cccgcgcgaa gtttgaggag
     6781 atgacggccg acctggtgga acgctgcatc ggccccttca agcaggcgct gtccgatgcc
     6841 aagatgaccg agaaggatct cgatcaggtc atcctcgtcg gcggagcgac gcgcatgccg
     6901 atgattcagg agctggtgcg caggctcacc ggcaaggagc ccaacaagga ggtgcacccc
     6961 gacgaggtgg tcgccgtagg ggcggctatc caggcgggcg tgctggccgg caccgtgcgc
     7021 gaggtcgtcc tgcttgacgt cacgcccctg tctttggggg tggagacgct tggcggtgtg
     7081 atgaccccac tcattgcgcg aaacacgacc attccaactc gcaagagcga ggtattcagc
     7141 actgcggcgg acggccagac gcaggtcgaa atcaacgtgc agcagggcga gcggccgatg
     7201 gcccgtgaca accgcacgct gggacggttc attctcgacg ggattccgcc ggccccgcgc
     7261 ggcattccaa agatcgaggt gacgttcgac atcgatgcca acggcattct cgacgtgaag
     7321 gcaaaggaca ccgcgaccgg gcgagagcaa tcgatcaaaa ttaccggcac ctcgaccctt
     7381 tcgaaggacg aagtggagaa gatggtcaag gagtcggagc gttttgcgga agaggacaaa
     7441 cgcaagcgcg acgccgccga ggcccgaaac cgcggtgact cgatggtcta ccagactgag
     7501 cgcatgctga aggaagccgg cgacaaagtc tcgtccgatg agcgccagtc gctcgaaacc
     7561 aaattgaccg ccctgaagga ggcgctcggc agcgatgatg tcgagcgcat caacgccgcg
     7621 atcaacgagc tgcagcaggc cagctacaaa ttggctgaag cgatgtatgc gaagaccgcg
     7681 gccagtgggg gtggggcatc ccccggcccc gagtcccagg gcccggcggg cgaaggcaag
     7741 tcggaagacg tcatcgatgc cgactacaag aagacggacg agaaacgata attgacggaa
     7801 agcacgacaa ggtggtccat ggacgagcga gaggatcgac ctaagaacga gggcctggag
     7861 acatccggca cgctgagcga tgcgaccgag acagttgagg agttgctcgc gaaggtgaag
     7921 cattataagg aagaggcgga acgcaactgg cagcagttcc tccacgccgc agccgatctg
     7981 gaaaactaca agaaacaggc cgcccggcag cgtgacgatg cagtgcagcg ggtgcgaact
     8041 tcgctcctga acgtcattct cacagtcgtc gacaacctgg accgcgccct tgagcacgcc
     8101 gacaaggcca gccgcgccga gaacatcctc gagggtattc gcatgacgca tagacaggtt
     8161 attgacctgc tccagagcct gggcgtccag tccatcgaca gtgttggcaa gcccttcgat
     8221 cccaagatgc acgaagccgt tgacgttgcc agcagccggg accactccgt tgatcctggt
     8281 accgtggtcg aggaactgca gaagggttac ctctttaatg gagaggtatt gaggccggcg
     8341 cgcgtgcgcg tcgcgaacta gccgtggaat tcaaagacta ttacaaaacc ttgggcgtcg
     8401 agcggtctgc tgatcagaaa gagatcagca agacgtaccg gcgcctcgcg cgtcagtttc
     8461 atccggacgt caataagaac aagggcgccg aggatcgctt caaagagatc aacgaagcgt
     8521 atcaggtgct gggcaagccc gaccagcgcg ccaaatatga tcagctgtac gaggcgtacc
     8581 agcacggtgg gatggactgg cagcaaatct tcggtcgcgg cggcgggtac cagacgccgg
     8641 aaggctggac ggtgactttc ggtggagacg ctgagaatct ggaggatgtt ctcggcgggt
     8701 tcagcgactt cttcaaacag ttcttcggcc aggagtttgc gacgacccgg actgcccgca
     8761 gtccctttgg acgacggcag accgttgacc gggcttacag ccgtcgcgcc ggtgtcgagg
     8821 agttgatgag cgagcaggag cctgccgccg ccgagtccat gcttgatatc acgctcgaag
     8881 aagcgttccg cggctcgcag aagccggtcg ccctgcagct gaacgggacg acccggcgcc
     8941 tggacgtcac aatccccaag ggtgtccgcg accggcagaa aatccgcctg tccggcgccc
     9001 tcgacggaag agatctctat ctgaccattc gtgtgttgcc gcatccgcaa ttcgaacggc
     9061 gggatgacga catcgtggtc gaggttcccg tcacgttgag cgaggcgatg ttgggagcca
     9121 agatcagggt gccgaccctc gaggggaccg tcgacatgac gattcctcct gagacacaga
     9181 atggacagat gttccgcttt cgggaacagg gcatgcccag gcgcgacggc ggccgagggg
     9241 atcagatggt tcgcgtgaag gtcgtgctgc cgaccaagct gtcggcccgc gagcggcagt
     9301 tgtttgaaga gctgagaaca ttgcgaaaag aagattcacg agaggcccga tagcatggcg
     9361 atccgttttg ataagttcac agaaaaagcc caagaggcga tactccacgc ccaggagacg
     9421 atgcagaaat tcgggcatcc tcaagtggag cccgagcacc tgctgctggc actgaccgag
     9481 caggccgaag gtgttgtgcc ggcaatcatc agccgggccg gcgcgaatgc ggccgcgctg
     9541 caggggcggc tgcgcaacga gttggggcgc cggccgaagg tccaggcccc gcagcaagct
     9601 gaaggactgt atttgagctc agcgctgcgg caggcgatcg agacggcgca gcaggaagca
     9661 cagcggatgc gcgatgagta cgtgagcacc gagcacctgc tgctggggat cgcccaggac
     9721 cgtgatggtc ttgcgggacg cctgctcgcc gaagccgggt tgacgcagga cgccatctac
     9781 cgggtgctgc aggaggtccg cgggagccag cgagtgacgg atcaaaaccc cgagagcaaa
     9841 tatcaggcgc tcgagcggta cggccgcgac ctgacccaac aggctgccgc cggaaaactc
     9901 gatccggtga taggccgcga cgaggagatc aggcgagtca ttcaggtgct cagccgccgc
     9961 acgaagaaca acccggtcct catcggagat ccgggtgttg ggaagacggc catcgtcgaa
    10021 gggctcgcgc agcgcatcgt gcgcggggac gtgcccgaag ggctgaagca caagcgcgtc
    10081 gtgcagctgg acatgggggc gctggtcgcg ggcacaaagt tccggggcga gttcgaggaa
    10141 cggctcaagg cggtgctcaa ggagatcgcc gacgccgccg gggagatcat tctgttcatc
    10201 gacgagctgc acacggttgt gggcgccggg gccgcggagg gcgcgatcga cgccgcgaac
    10261 atgctgaagc cgacgctggc gcgtggagaa ctgcggacaa tcggcgccac gaccctggac
    10321 gagtaccgca agcacattga gaaggacgcc gcattggagc ggcgcttcca gccggtgctg
    10381 gtcggagagc catcggtcga ggaaacgatt tcgatcctgc gagggcttaa ggaacggtac
    10441 gaggtgcacc acggcgtccg catcaccgac gcggcggtca tcgccgcgtc gacgctgtcc
    10501 gaccggtaca tcagtgatcg cttccttccc gatcaagcga tcgacctcat cgacgaggcg
    10561 gctagccgcc tccggatgga gatcgatagc aagccggtgg agctggacga gatcgatcgc
    10621 cggatcatgc agctggagat cgaaagacag gcgctgcgca aagaacgcga ccctgggagc
    10681 caggagcgac ttcaacgact cgagcaggaa gtggctaagc tcaaggcaga gagcgaacaa
    10741 ctgcgcaagc agtgggaaga agagaagcgc gcgatcagcg caatccgcga gaccaaggag
    10801 cggatcgagg agacgcggca gcagatcgag gaggccgagc gccgcgcgga tctcgaagcc
    10861 gctgcgcggc tgcgctatgg cacgctccgc gaacttcagg agcgaatggc gtcgcaggag
    10921 caggcgctca aagacattca ccggggccga cacctcttga agcaggaagt agatccggat
    10981 gatgtcgctc aggtcgtcgc caagtggacc gggattccgg tgacgcggct gctcgagggt
    11041 gaaatgcaga aactgctcac catggaacag cgtctacacg agcgcgtggt gggacagaac
    11101 gaagccgtgg ccgcggtgag cgacgcgatc cggcgggcca gggcgggcct gaaggatccc
    11161 cgccggccca tcggttcatt cttgttcctg gggccaacgg gggtggggaa gaccgaactg
    11221 gctcgagcgc ttgcgcagca catgttcgat gacgaagagg cgatgatacg cctcgacatg
    11281 tccgagtacc aggaaaagca caccgtcagc cgcctgatcg gcgcgccgcc cggctacgtc
    11341 gggttcgacg agggtggcca gctcacggaa gcagtgcggc ggcggccgta tcgtgtcgtg
    11401 ctcttcgacg agatcgagaa agcgcacacg gacgtcttca atatcctgct acagattctc
    11461 gaagacggcc ggttgaccga tgggcatggc cgcaccgtcg atttcaagaa cactgtcatc
    11521 atcatgacca gcaacctcgg cagccggttc cttcagcatc tcgacgccag ggacgccgcg
    11581 cagttcgaga tggcgcgggt ccaggtgctg gacgaagtgc gaaaggcgtt ccggcccgag
    11641 ttcctgaatc ggatcgatga gatcatcatc ttccacccgc tgtcggcaga gcagctgcgg
    11701 cagatcgtgg acctgcagct ccgctatctg catgaccgtt tggcctcgca gaagatggga
    11761 ctggaagtca tcgacgccgc gaaggactat ctcgcgcgcg aggggtacaa tccggacttt
    11821 ggcgcgcgac cgctgcggcg tctcattcag cgtgaagtcg agaaccgcat cgccaggatg
    11881 gtgctggccg ccgagttgag agaaggattc gccgccgtcg tggactacaa gcacggcgag
    11941 atcaagatca ccatccgcga gcgcgtggcg gtcacttcga agtaaactga ggaacccccg
    12001 attcttggga tcacggcctt tgtgagcggc gcggtcctcc ttgcgcttga gatcgtcgcg
    12061 agtcgcatca tcgctccgtt tttcggcaac tccatctacg tgtggggcag cctgatcgga
    12121 gtctttctcg ccgctctcgc gctggggtat tttctcggcg gccaggcagc cgaccggtgg
    12181 ccgtcgttgg cggtcctcgc cgggtgcgtg ttccttgcgg gcgcgctcgt ggttccaatt
    12241 cctgtcgtcg cgccccgggt gcttgaggtg atcattctgc gcgatccggg gccgcgcata
    12301 agcccgctgg tcgggtcgct cgccctgttc tttctccctg gtgtggtcat cgggatggtg
    12361 tcgccctttg cggttcgtct gcgggctcgg acggtggaca caattgggaa tgtcgctgga
    12421 gcgctctatg ccctctccac gctcggcagt atcgccggga cgctgcttgc gtcgtttgtg
    12481 ctgattgccg tgacggacgt cagccagatc gtctatgcgc ttggggctgc actgctcgtg
    12541 ctggcc
//
LOCUS       DBNGSB010000194        10311 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2441188, whole genome shotgun sequence.
ACCESSION   DBNGSB010000194 DBNGSB010000000
VERSION     DBNGSB010000194.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 10311)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10311)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10311
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2441188"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..122
                     /locus_tag="AC159B_05620"
     CDS             <1..122
                     /locus_tag="AC159B_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3377257.1"
                     /translation="RVAKYNQLLRIEEELGDRAVYPGATVFKRRERGTGNREQ"
     gene            216..587
                     /locus_tag="AC159B_05625"
     CDS             216..587
                     /locus_tag="AC159B_05625"
                     /inference="COORDINATES: protein motif:HMM:NF016839.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsB family cell division protein"
                     /protein_id="HIR3377258.1"
                     /translation="MDRIARWFVSSLILALMIAVTYAFGSSFLQVYRLTREAARLEQV
                     KRYLIEHNAQLREEIKLLHTPGYVERIAREQLGLVKPGEVALLIVRPKPPAPRSAGPR
                     AAAKPARNGPVWRPWLQRLGF"
     gene            683..1060
                     /locus_tag="AC159B_05630"
     CDS             683..1060
                     /locus_tag="AC159B_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HIR3377259.1"
                     /translation="MAVEAGTIVEGTVVKITHYGAFVELPDGKSGLVHISEIADAYVK
                     DVRDYIKEQERIKVKVLGINEKGKLDLSLKQAFSPEERAVRARAKTSFEDKLKAFMKE
                     SEERLLDLKRNTEAKRGGGKKRK"
     gene            complement(1193..1534)
                     /locus_tag="AC159B_05635"
     CDS             complement(1193..1534)
                     /locus_tag="AC159B_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3377260.1"
                     /translation="MDDQRNLEAAKTVIVRAVESRRELVAYSKLEAIEMDRYARDVER
                     GALEELGKLLPSAPAGEPLYQVKTKLQWMEQQLAELDGRKDIQERSRALERDEITWRA
                     FEDISWLLGVL"
     gene            complement(1534..3441)
                     /locus_tag="AC159B_05640"
     CDS             complement(1534..3441)
                     /locus_tag="AC159B_05640"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3377261.1"
                     /translation="MSMHFQEDEILGKAYDARLMRRLLRYLRPYRPIVVVSVVLLLLA
                     AAADLVGPYLVKVAIDRYIRNGDLAGVTRIALYYLVALVAGAAVRYGQNYITQTVGQR
                     VMYDLRMELFTHLQRLSVAFFTRNPVGRLMTRITNDIDALYEMVTSGVVTVFGDFFML
                     AGIIVAMLVLNWRLALVTFVVLPALVGMTMWFQVRSRESYRAVRIRIARINAYLNENI
                     MGMSIVQLFNRERRNFGRFDELNRDHLASHRQAIRGFALFFPSVGFISAVATALLIWY
                     GGGQVIQNAVTLGVLVAFIQYTERFFRPIQDLAEKYNILQSAMASSERIFGILDEPES
                     VQDPVDPLPLERVRGEVEFRDIWFAYPAAEYAGGDGATHASRSDGPRTAMVRGEPPVP
                     DGTDEWVLKGVSFKIHPGESVAVVGHTGAGKTSIINLISRFYDPQRGQVFVDGVDIRR
                     YPQRDLRRHVGLVLQDVFLFSGTIASNIRLGNAEISDDEVRRAAEFVNAHRFIERSPN
                     GYAGEVTERGSTLSAGQRQLIAFARAISHNPEILLVLDEATSSVDTETEILIQDALSK
                     VLRGRTSIIIAHRLSTIQNVDRIIVMHKGRIVEEGTHRDLLARGGIYTKLYQLQYRDQ
                     ELRLPKLRTPS"
     gene            complement(3438..5186)
                     /locus_tag="AC159B_05645"
     CDS             complement(3438..5186)
                     /locus_tag="AC159B_05645"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3377262.1"
                     /translation="MSPRLLSYILRHRRVYLFGFMLGTTGSALLMLDPIVLRLAVDGI
                     GRGVSSRLLLMYGGVLVGIHLVVWILRYFWRLLILGVSRQIEYEIRNDYFAHLQRMDL
                     AYFQHTRTGDLMTRAVSDLNTVQRFAGPGVMHFFSTIVSSIIAIGFMLSIDARLALFM
                     LVTLPLVSATFIVLGPMIHRRYESVQDQFSTISAKAQENFSGIRVVKAFAQEPFEVDE
                     FARLNREYIHRNLAVIKVDGALWPLMMFFIGLSSMVLLWQGGQAVITGRITLGQFVQF
                     NGYLALLAWPMIALGWVVNLMQRGLASMKRLDEVFAQRPRITDAPDARTPAAARGDIE
                     FRDVSFAYNGAQVLRDVNLRIPAGTTVAIVGPTGAGKSTMVGLIPRLFDATQGEVRVD
                     GIDVRRWRLADLRRHIGFVAQDTFLFSETLSENIGYGMERADGGRITEAAEVSRLAAD
                     VAEFPHGYNTVLGERGVTLSGGQKQRAAIARAVARDPRILILDDALNSVDTNTEEQIL
                     ARLRDVMATRTAILISHRISTVRHADQIIVIDDGRIVERGTHDELLARGGLYADLYEK
                     QLLEEALEETDVETST"
     gene            complement(5197..6018)
                     /locus_tag="AC159B_05650"
     CDS             complement(5197..6018)
                     /locus_tag="AC159B_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HIR3377263.1"
                     /translation="MSGYLTTAIAFIRDNGDELERARLTGVLGRTRPDPKAARTLLGR
                     QHDDGGFPYGMISGRPSAVTATATALQWMVDLRLLPSPHVERAIGFFLTTQRPDGSWD
                     ESPAVLKFDPPPHTRPGHPVGRAYCTALAGFWMTRLTGSRHDAVQRTAAYLRARRNGD
                     QPPPELLTIDALEVATLAMVEGKTADLVRAGVTALGDVGEERWNADALASALAALYAA
                     GFGDDEPFVAWAIPQLLSLQRPDGEWSSASGQDQDVDLSLQALGALLAFGLPSAV"
     gene            complement(6137..6895)
                     /locus_tag="AC159B_05655"
     CDS             complement(6137..6895)
                     /locus_tag="AC159B_05655"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HIR3377264.1"
                     /translation="MRAVRIDLNSDLGEGAGTDEQLLPLISSANIACGGHAGDKVSMA
                     KTVRLAVEYGVGVGAHPSFPDREGFGRRAIRMSPEELRDTLTSQMDALQAVVDRFGVS
                     VQHVKVHGALYNLAAADPNLAALIGETVRAINPGVIVVALAGSVMADILERIGVRVAR
                     EAFIDRGYAADGTLISRDRAHAIVAEPHEAAFRAVRMARDGLVAAEDGTEIGVQPDTL
                     CIHGDNPSAVLLARAVRRALDEAEITVTPMASLV"
     gene            complement(6992..7252)
                     /locus_tag="AC159B_05660"
     CDS             complement(6992..7252)
                     /locus_tag="AC159B_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3377265.1"
                     /translation="MKISDADAATRLQSAVSRVDSRLELDRGAIRYVTEPFPGIEYGL
                     RLGDAGALLFMPEGDLTAPDWETRLFKRLEAAKKYLEEFPPR"
     gene            7407..7967
                     /locus_tag="AC159B_05665"
     CDS             7407..7967
                     /locus_tag="AC159B_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR3377266.1"
                     /translation="MNWATQFQQALPYQEFLTRHGTDAHRQRWQAVYDRIQLTDQQRV
                     LLGGFPRKMHLLCVAGTWCGDCVRDGPILQRITEAATTVELRFLDRDQRPEISQELSI
                     NDGLRIPMVLFLSEDHIECARYGERTLATYRKMAAERLGPACPTGVVPPGEEYLATVT
                     QEWLDEVERIQLLLRLSARLRALHND"
     gene            8002..8079
                     /locus_tag="AC159B_05670"
     tRNA            8002..8079
                     /locus_tag="AC159B_05670"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8038..8040,aa:Glu,seq:ctc)
     gene            complement(8359..8631)
                     /locus_tag="AC159B_05675"
     CDS             complement(8359..8631)
                     /locus_tag="AC159B_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3377267.1"
                     /translation="MANELEKYRMLISQYLGEMGRDDIDVGLVEEIDQGRLRVEFIKG
                     PMRRDAEVPVYDLKNPEQARSAINIALLQLSKAVAKEHIAEAKEGN"
     gene            8766..9443
                     /locus_tag="AC159B_05680"
     CDS             8766..9443
                     /locus_tag="AC159B_05680"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3377268.1"
                     /translation="MLSLVTDLAYLVTALWESATLRGVIRLWSELRYRTSGTIYAGHI
                     ARTEDYGAPLRDALQTLPAHPRRFLDVSTGTGCAAHVAMSKFPEARSYACDLSTSMLS
                     AARVNLPRTALIRCDSAHLPFGNGAFDLVLLQNAPPPLSELARVTAPGGWVVLGFSAA
                     GSLPAWLPEEIVRRLAGYGLRGARWIRAGRGLYIVGRLDVLEDPVSHPGLDKVDSVSY
                     AQQGVHS"
     gene            9440..10075
                     /locus_tag="AC159B_05685"
     CDS             9440..10075
                     /locus_tag="AC159B_05685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3377269.1"
                     /translation="MKVSMVIAALDEAQTVADVVRAGRRCRRVQEIIVVSDGSRDRTV
                     AVARQAGADVIELPRNVGKAGAVMVGVERAAGDIVVLLDADLIGLRPDHVDELLHPVL
                     DDGIDMVIGVMKRDLVQNVLPSFSGQRAIPRFHLIRHSELRDKGFGLERALASIARRE
                     RWRVRRIELRELSHRLKEEKYGLVRGYRAKLKAAEDLVRHTRLRRGRRIGT"
     gene            10072..>10311
                     /locus_tag="AC159B_05690"
     CDS             10072..>10311
                     /locus_tag="AC159B_05690"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HIR3377270.1"
                     /translation="MTLAPSPTPSDRILLIVAHTDDEVIAAGGYLAAASEAGSEITVV
                     ILTNGDANRLSAALLGRTFRPSHHAFVREGEIRQQE"
BASE COUNT         2015 a         3319 c         3092 g         1885 t
ORIGIN      
        1 agcgtgtggc gaagtacaat caactcctaa gaattgaaga ggagctcgga gatagagcgg
       61 tctatccggg agcaacagta ttcaaaagac gggaacgggg aacagggaac agggaacagt
      121 agaagaacca atgccagcga tggggccggg accgttcgcc gttccccgat ccccgttccc
      181 cttcctatac gtcgcacttt tcgtcggagc agcttatgga ccgcatcgcg cgatggttcg
      241 tcagctcact catcctggca ctgatgattg ccgtcaccta cgcgtttgga tccagtttcc
      301 tccaggtgta ccggctcaca cgggaagctg cccgcctgga gcaggtgaaa cgatacctca
      361 tcgagcacaa cgcccaactc cgcgaggaaa tcaagctcct gcacactccc ggctacgtcg
      421 agcggatcgc ccgcgaacaa ctcgggctgg tcaaaccggg cgaggtggcg ctcctaatcg
      481 tccggccgaa gccccccgcg ccgcgctccg ccggaccccg ggccgccgca aagccggccc
      541 ggaacggccc ggtttggcgg ccctggctcc agcggctggg gttctagatg gtactaattg
      601 acacatttga gagggcagcc gtataattcg cgtgcatccc acgtagttca acctggtcaa
      661 ggaggtatag ccgggcaaag tcatggcggt ggaagctggt acgatcgtcg aaggcacggt
      721 cgtcaagatt acgcactacg gcgcgttcgt agaactcccc gatggcaaga gcggtctcgt
      781 ccatatttct gagatcgccg acgcatacgt caaggacgtc cgggactaca tcaaggagca
      841 ggaacggatc aaggtcaagg tcctgggcat caacgagaaa ggcaagctcg acctctccct
      901 caagcaggca ttctcacccg aagagcgcgc cgtccgggcg cgcgcgaaga cgtcttttga
      961 ggataagctc aaggcgttca tgaaggagag cgaagagcgc ctcctggatc tgaagcgcaa
     1021 taccgaggcg aaacgcggcg gcggcaagaa gcgcaaataa cggcgtctac ccggtctccc
     1081 cgtctgcccg gtcttccccg tctctgacca aatcaccaat tcagttccat agggattctt
     1141 ttctttagac gggggagacc gggaagacag ggtagacggg gaagacgatt gactacagaa
     1201 ctcctaacaa ccaagagata tcttcgaaag cgcgccaggt aatttcgtca cgctcgagcg
     1261 cgcgcgagcg ttcctgaata tccttccgcc cgtccagttc cgcgagttgc tgttccatcc
     1321 attgaagctt ggtcttcact tgatagagcg gctcgccagc gggcgcgctc ggcaagagtt
     1381 tgccgagctc ttcgagagcc ccgcgctcca catcccgcgc gtagcgatcc atttcgatcg
     1441 cttccagttt ggaataggcc accagttccc ggcgagattc cacggccctt acgatcacgg
     1501 ttttggccgc ctcgagattt cgctgatcgt ccatcaggac ggcgttcgca gcttcggcag
     1561 ccgcagctcc tggtcgcggt attgcaactg gtacaacttc gtgtagatcc cccctcgggc
     1621 caggagatcg cggtgtgtgc cctcctccac gatcctgccc ttgtgcatca cgatgatccg
     1681 gtcaacgttc tgaatcgtag agagccggtg cgcaatgatg atcgaggtcc ggccgcgcag
     1741 caccttgctc aacgcgtctt gaatgaggat ctctgtttcc gtatcgacgc tgctggtcgc
     1801 ctcgtccagc accagcagga tctccggatt gtgcgagatc gcgcgggcaa aagcgatcaa
     1861 ctgccgctgg ccggccgaca aggtggatcc gcgctcggtc acttcgcccg cgtagccatt
     1921 gggtgaccgt tcaatgaatc ggtgcgcgtt cacgaactcc gcggcgcgcc tgacctcgtc
     1981 gtcgctgatc tcagcgttgc cgagccggat gttcgacgcg atcgtgccgg agaacaagaa
     2041 cacatcctgg agcacgaggc cgacatgcct gcggagatcg cgctgtggat accggcggat
     2101 atcgactccg tctacgaaca cctgaccgcg ctggggatcg tagaacctgc tgatcaaatt
     2161 gatgatcgat gttttaccgg cgccggtatg cccgaccacc gccacgctct cgccgggatg
     2221 gatcttgaat gacacaccct tgagcaccca ttcgtcggta ccgtccggca ccggcggctc
     2281 gcctcgaacc attgcggttc ggggcccgtc cgatcgggac gcatgtgtcg ctccgtctcc
     2341 tcccgcatac tcggccgcag gataggcgaa ccaaatatcc cggaattcga cttcgccccg
     2401 cacgcgctcc aggggcaggg ggtccaccgg atcttgcacg ctttcgggct cgtcgaggat
     2461 gccgaagatc cgctccgagc tcgccatcgc cgactgcagg atattgtact tttccgcgag
     2521 gtcctgaatc ggtcggaaga agcgctccgt gtactggatg aacgccacca gaacgccgag
     2581 ggtgaccgca ttctggatga cctgcccacc tccgtaccag atcagcagcg ccgtggcgac
     2641 cgccgaaatg aaccccaccg atgggaagaa cagcgcaaac ccacggatcg cctgtctgtg
     2701 cgatgccagg tgatctcggt tcagctcatc gaaccgcccg aaattccgcc gctcgcggtt
     2761 gaacagctgg acgatcgaca tccccatgat gttctcgttc aggtaggcgt tgatccgcgc
     2821 gatccggatg cggacggcgc ggtacgactc tctggagcgc acctggaacc acatcgtcat
     2881 gcccacgagc gccggcagca ccacgaacgt cacgagcgcc agccgccagt tcaacaccag
     2941 catcgcgacg atgatgccgg ccagcatgaa gaaatctccg aagaccgtca ccacccccga
     3001 tgtcaccatc tcatagagcg cgtcgatgtc gttcgtgatc cgcgtcatca gacggcccac
     3061 aggattccgg gtgaaaaatg ccaccgacag acgctgcagg tgcgtaaaca gttccatccg
     3121 caggtcgtac atgacgcgct ggccgacagt ctgcgtgatg tagttctgcc cgtaccgcac
     3181 ggccgcaccc gccacaaggg cgaccaggta gtatagggcg atgcgggtga cgccggcgag
     3241 gtccccgttc ctgatgtagc ggtcgatggc gaccttcacc aggtaagggc cgaccagatc
     3301 ggcggccgcc gccagcaaca acaggacgac cgagacgacg acgatcgggc gatacggacg
     3361 caggtaccgg agcagacggc gcatcaaccg cgcgtcgtac gccttgccga gaatctcgtc
     3421 ttcctgaaag tgcatgctca tgttgaggtc tcgacatcgg tttcttcgag cgcttcctcg
     3481 agcaactgct tctcgtacaa gtccgcgtac agcccgcccc gagccagcag ctcatcgtgg
     3541 gtgccgcgct ccacgatacg gccgtcatcg atgacgatga tctgatccgc gtgccggacc
     3601 gtgctgatgc gatggctgat caggatcgcg gtgcgggtcg ccatcacatc gcggaggcgg
     3661 gcgagaatct gctcctcggt attcgtgtcc acgctgttga gggcgtcgtc aaggatcagg
     3721 atgcgcggat cccgggcgac cgcccgcgcg atggccgcgc gctgcttctg cccgccggag
     3781 agcgtcacac cgcgctcccc cagtacagtg ttgtacccgt gcggaaactc ggcgacatcc
     3841 gcggccaggc ggctgacctc ggccgcctcg gtgatgcgcc ccccatccgc gcgctccatc
     3901 ccgtacccga tgttttcgct gagcgtttca gaaaacagaa acgtgtcctg cgccacgaac
     3961 ccgatgtgac gccgcaggtc tgccaggcgc caccgcctca cgtcgatgcc gtcaacgcgc
     4021 acctctccct gcgtcgcatc gaacaggcgg gggatcaatc cgaccatggt gctcttcccc
     4081 gcgcccgtcg gtcccacgat cgccaccgtg gtacccgcgg gaatccgcag attgacgtcg
     4141 cgcagtactt gcgcgccatt gtacgcaaag cttacgtcgc ggaactcgat gtcgccgcgt
     4201 gctgcggcgg gcgtccgggc gtccggagca tcggtgatcc tcggcctctg cgcaaagact
     4261 tcatcgaggc gcttcataga cgccaggccg cgctgcataa gattcaccac ccatcccagc
     4321 gcgatcatcg gccatgccag cagggccagg tagccattga actgcacgaa ctggccgagc
     4381 gtgatgcggc cggtgatgac ggcctggccc ccctgccaca acagcaccat cgatgacagt
     4441 ccaatgaaaa acatcatcag cggccacagg gcgccgtcga ccttgatgac cgccaggtta
     4501 cggtgaatgt actcgcggtt caagcgggcg aattcgtcga cctcgaaggg ttcttgcgca
     4561 aaagccttca cgacgcgaat cccagaaaaa ttctcctgcg ctttcgccga gatggtgctg
     4621 aattgatcct gcaccgattc gtaacggcgg tggatcatcg ggcccaacac gataaaggtg
     4681 gcgctcacga gcgggagcgt caccagcatg aacagcgcca gtcgcgcgtc gatcgaaagc
     4741 atgaacccga tggcgatgat tgagctcacg atcgtgctga agaagtgcat gactccaggg
     4801 cctgcgaacc gctgcaccgt attgagatcg ctgactgcgc gcgtcatcag atcgcccgtc
     4861 cgggtgtgct ggaagtaggc gagatccatg cgctgcaggt gcgcgaagta gtcgttgcgg
     4921 atctcgtatt cgatctgacg cgataccccg aggatgagga gccgccagaa gtaccggagg
     4981 atccagacta cgaggtggat ccccacaagg actcctccgt acatgagaag cagacgcgaa
     5041 gagacccccc ggccgatgcc gtccaccgca agacgcagga cgatcgggtc gagcatcagc
     5101 agcgccgagc cggtggttcc cagcatgaag ccgaacaggt agacccggcg gtgtctcagg
     5161 atgtatgaga ggagacgagg gctcaaagcg ttccgattat actgccgacg ggagaccaaa
     5221 cgccaggaga gcgccgagcg cctgaagcga aaggtcgacg tcttgatcct ggccggacgc
     5281 gctggaccat tcgccatcgg ggcgctgcag cgagagtagc tgcgggatag cccaggccac
     5341 aaaaggctcg tcgtcgccga agcccgctgc gtagagtgcg gcgagcgcag aggccagcgc
     5401 gtccgcgttc cagcgttcct cgccaacatc accaagcgct gtcacacctg ccctcacgag
     5461 gtcggccgtt tttccctcaa ccatcgccag cgtagcaacc tcgagagcgt caatggtcaa
     5521 caattcgggg ggaggctggt cgccgttgcg ccttgcccgc agataagcgg cggtccgctg
     5581 taccgcgtca tgacgggagc ctgtgagccg cgtcatccag aaccccgcaa gtgcggtgca
     5641 gtatgcgcgc ccaactggat gtcccggtcg agtgtgcggc ggaggatcga atttcaggac
     5701 tgcgggactc tcgtcccagg agccatccgg gcgctgggtg gtgaggaaaa agccgatcgc
     5761 acgctcgacg tgggggctcg gcagtagccg caggtctacc atccactgga gcgccgtcgc
     5821 cgtcgccgtc actgcggacg gacgaccact gatcattcca tacgggaaac caccatcatc
     5881 gtgctgcctg cccagaagcg ttcgcgccgc tttcggatcc gggcgggtac gacccaatac
     5941 ccccgtcagg cgagcgcgtt caagctcgtc gccattgtcc cggataaacg cgatggccgt
     6001 cgtcaagtag cctgacactc agcgtctccc ccgtctcccc cgtctgccca gtctgccccg
     6061 tctcctagat atcatcccca gttcagatga tgagactggg aagacgggga agactgggtg
     6121 gactgggaag acgcaactat acaagggatg ccatgggtgt cacggtgatc tcagcttcat
     6181 caagagccct gcgaacggcc cgcgcaagga gcactgcact gggattatcc ccgtggatgc
     6241 acagagtatc tggttggacc ccaatctcag tcccgtcctc tgctgcgacg agaccgtcgc
     6301 gcgccatacg gaccgctcga aacgctgcct cgtgtggctc tgcgacgatg gcgtgggcgc
     6361 ggtcgcgaga gatcagggtg ccgtcggccg cataacctcg atcaatgaag gcctctcgag
     6421 ccactcgcac cccgatgcgc tccaggatgt ccgccatgac tgaaccggcg agcgcgacca
     6481 caattacccc tggattgatg gctcgcaccg tttcaccgat caaggcggca aggttcggat
     6541 cggcagccgc cagattgtac agggcgccgt gtaccttgac atgctgcacg cttaccccaa
     6601 agcggtcgac aactgcttgg agggcgtcca tctgacttgt caatgtatcg cgtaactcct
     6661 cagggctcat cctgatcgcc cgacgcccga aaccttcacg gtctggaaac gatggatggg
     6721 cgccaacgcc gacgccgtac tcgaccgcga ggcggacggt cttcgccatt gacactttgt
     6781 cgccggcgtg gccgccgcac gcgatattcg ccgaggaaat gaggggcagc aactgctcgt
     6841 cggtgccggc gccctcgccg aggtctgagt taaggtcgat gcgaacggcc ctcacttaac
     6901 ccggcaccaa gcaaaattcc cccggcctcg gccctcgctc ggcctctgtg cgccgccggc
     6961 ccactccgtg ggcagcattg gtggtcgcca tctatcttgg tgggaactcc tccaggtact
     7021 ttttggcggc ctcgagtcgc ttaaacaggc gcgtctccca gtcgggggcg gtgagatctc
     7081 cctccggcat gaaaagcagt gcgcccgcgt cgccgaggcg cagtccgtac tcgatcccgg
     7141 gaaacggttc tgtcacgtag cggatcgcgc cccgatcgag ctcaagtcga gagtccacac
     7201 gggacacggc cgactggagc cgtgttgcgg catcggcatc tgagatcttc attgaacccg
     7261 gcaccaagca aaattctccc ggcctcggcc tagctcggcc tctgtgcgcc gtcggcccac
     7321 tccgtgggcg atattagtgg tcgctactct attctacgga atacacgaac aagggaacag
     7381 gagagcaggc gaacaacaaa cgcagtatga actgggcaac gcaattccag caggcgctgc
     7441 cgtatcagga gtttctcact cggcacggca ccgacgcgca ccgtcagcgc tggcaggcgg
     7501 tatacgaccg catccagctc acggatcagc agcgcgtgct gctcggcggt ttcccccgaa
     7561 agatgcacct gctttgcgtc gcgggcacct ggtgcggcga ctgcgtccga gatggaccga
     7621 tcctccagcg gatcacagag gcggccacga ccgtggagct gcgctttctc gatcgagacc
     7681 agcgcccgga aatctcacag gaactgtcca ttaatgatgg gcttcgcatc ccgatggtgt
     7741 tgtttctcag cgaagaccac atcgagtgcg cgcggtacgg agagcgtacg ctggcgacat
     7801 accggaagat ggccgccgag cgactcggtc ccgcctgtcc gaccggcgtg gtcccgccgg
     7861 gtgaagagta tctggcgacg gtcacgcagg aatggctgga cgaagttgag cgaatacaac
     7921 tgttgcttcg attatcagcg cgactgcgcg cccttcacaa tgactagacc ggcgtgttcc
     7981 gatataattg ggtgcggtcc cggccccatc gtctagcctg gcctaggaca cagccctctc
     8041 aaggctgaga cacgagttca aatctcgttg gggctaccaa agtgggccag ctttcgtggg
     8101 ctcctaggcc tctccccgaa aagaaactac cacggggacc cctctagcct aggagcccac
     8161 ggcgctggcc aatcactttc tccgcttagg cgaatagcgc gggtttgcac ggtccaaacc
     8221 attaggccgc gatcgtcggg gacggagcct gaggggtccc cgtatcagtt tcagtcgggg
     8281 agaggctccg accccgatgg gtgcggccgc ctacttcccc ctcggagccc ccgaaagctg
     8341 gccacgtttc atgttctgtc agttaccttc cttcgcttct gcaatatgct ctttcgcaac
     8401 tgccttgctc aactgcagca acgctatgtt gatggccgac cgcgcctgtt caggattttt
     8461 aagatcgtag accggtacct ccgcatcgcg acgcatcggc cctttgatga actcaactcg
     8521 aagccggccc tgatcgattt cctccacgag cccgacatcg atgtcatccc gacccatttc
     8581 gcccagatac tggctgatta acatccggta cttctcaagc tcattggcca tggcacatcc
     8641 ctcctccgcc gccattttcc cagatcggcg gggtctcaca ccctatgtcc ggagaggaag
     8701 gctgtgcaaa ccagcgaacg catgagtgat ttgcgacgcc gcgccctgga gggcacgata
     8761 ccgccttgct gtcgctggta accgacctgg cgtatctcgt gacggcgctt tgggagtcgg
     8821 ccaccctccg gggagtcatt cgcctctggt cggaactccg atatcgaacc tcgggcacga
     8881 tctacgccgg ccacatcgct cgcaccgagg actatggggc gccgctccgg gacgcgttgc
     8941 agaccctgcc ggcacatccc cgccgcttcc tcgatgtaag tactggaacg ggatgcgcgg
     9001 cgcacgtcgc gatgagcaag tttccggagg cacgttccta tgcctgcgat ctttcgacat
     9061 cgatgctctc agcggcaagg gtgaaccttc cgcgcaccgc cctgatccgt tgcgacagcg
     9121 ctcacctgcc gtttggaaac ggcgcatttg atctcgtcct cctacagaat gctccgccgc
     9181 cgctgagcga gctggcccgc gtgacagcac ctgggggctg ggtggtactt ggcttctcag
     9241 ctgccggcag cctcccggcg tggctgccgg aggaaatcgt ccggcggttg gcaggctacg
     9301 gtctccgcgg cgctcgttgg atacgcgccg gccgcggcct gtacatcgtg gggcgcctcg
     9361 acgtcctcga ggatccagta tcccaccccg gcttggacaa ggtggacagc gtcagctacg
     9421 cgcaacaggg agtgcactcg tgaaagtctc catggtgatc gccgcgctcg acgaggcgca
     9481 gaccgtcgca gacgtggtgc gcgcgggtcg ccggtgccgc cgagtacaag aaattatcgt
     9541 cgtctcagat ggctctcgtg atcgaaccgt cgccgtcgca cggcaggcgg gagccgacgt
     9601 tatcgaattg cctcgaaatg tcggcaaggc aggcgcggtg atggtcggcg tggaacgtgc
     9661 ggccggcgac atcgtcgtgc tgctcgacgc ggatttgatc ggactgcgcc cggaccatgt
     9721 cgatgaattg ctgcaccctg tgctcgacga tggcatcgac atggtgattg gagtcatgaa
     9781 gcgcgacctg gtgcagaatg tgctcccttc gttctctggc cagcgcgcga tccctcgatt
     9841 ccatctgatc cggcattcag agctacgtga caaaggattt ggcctcgagc gcgctctggc
     9901 ctcaatcgcc aggcgggaac gatggcgggt ccgcaggatt gaactgcgcg aactctctca
     9961 ccgcctgaag gaagagaagt atggcctggt gcgcgggtac cgtgcgaagc tcaaggccgc
    10021 cgaagacctc gtccgtcata cccgccttcg ccgtggccgc cgaataggca cgtgacgctg
    10081 gccccttccc ccacgccatc ggatcgcatt ctccttatcg tcgctcacac cgacgacgag
    10141 gtcatcgcgg caggcggcta tctggcggcg gccagtgaag ccggaagcga gatcaccgtc
    10201 gtcatcctca caaacggcga cgccaaccgc ctctccgcag cgctactggg gcgcacattc
    10261 cgacccagcc atcatgcgtt cgtccgcgag ggagagattc gccagcagga a
//
LOCUS       DBNGSB010000195         9256 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_756054, whole genome shotgun sequence.
ACCESSION   DBNGSB010000195 DBNGSB010000000
VERSION     DBNGSB010000195.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 9256)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9256)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9256
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_756054"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..326
                     /locus_tag="AC159B_05695"
     CDS             42..326
                     /locus_tag="AC159B_05695"
                     /inference="COORDINATES: protein motif:HMM:NF013821.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HIR3377271.1"
                     /translation="MYAGFARVPSGAGSTPGSQAAISVGTRPTFGPGELLIEAHLIDV
                     SVDLYGAQIDVQFVERLRDELKFASIEELIRQMHADVEQARVILVTPEIR"
     gene            541..810
                     /gene="rpsO"
                     /locus_tag="AC159B_05700"
     CDS             541..810
                     /gene="rpsO"
                     /locus_tag="AC159B_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HIR3377272.1"
                     /translation="MALRKAAKTEIVTKYKRHQEDTGSPEVQIALLTERINQLTEHLG
                     THKKDLHSRRGLLKMVGQRRGLLTYLMRSDPARYRQIVDNLGLRR"
     gene            827..3049
                     /locus_tag="AC159B_05705"
     CDS             827..3049
                     /locus_tag="AC159B_05705"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013986612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIR3377273.1"
                     /translation="MTPVEQTPVQRTEVDLAGRTFSLETGWLAKQANGSVVVRYGESV
                     VLVTATMSETLREGIDFFPLTCDFEEKMYAAGKIPGGFFKREGRPGERAILSGRLMDR
                     PIRPLFPKGFRNDVQVIATVLSTDQENDPSILAVNGASAALTISDIPFDGPIGAVRVG
                     LVNGELVVNPTMKQIDEESDLDFIVAASEDAVVMVEAGANEVTEERLLEALDLAHREI
                     RRIIGAQRELASRAGRAKVSVQLATPDPELDAAVRGRALPLIKSALSQPEKLMREDAL
                     RQVAEQVAPAILEQYPERAKEIEEVIASATKEEVRRMILDEGRRPDGRGVRDIRPLTA
                     TVGILPRAHGSGLFQRGQTQVLSVATLGTGEDEQIIDDISLRKSKRFMHHYSFPPFSV
                     GEVRPLRGAGRREIGHGLLAERALERMIPDQTVFPYTIRLVSEILESNGSTSMGSVCG
                     STLALMDAGVPIKAPVGGISIGLVTGQQGKTALLTDIQGIEDAMGDMDFKVAGTRAGV
                     TALQLDVKNKGLSRDVTRQTFQQAREARLTILDAMARAIAAPRGELSRYAPRIITIEI
                     NPDKIREVIGPGGKVINKITAETGVKIDIEQDGRVLIASVDEEAARKAREMIEAIVRE
                     AKVGETYTGRVTRLMNFGAFVEVLPGKEGLVHISELAEGRPERVEDVVKVGDELTVRV
                     KEIDSQGRLNLTRRGLPGGPTGEAAPVPSGDRRPRRDDRRFGDRHRRRGPRPDSPNQP
                     "
     gene            3106..4407
                     /locus_tag="AC159B_05710"
     CDS             3106..4407
                     /locus_tag="AC159B_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3377274.1"
                     /translation="MSELINKTTLPNGIRVLTERMPHVRTAAMGIWVGAGSRYEPAPQ
                     HGITHFLEHLFFKGTEKRSALEIAQTADDMGGQMNAFTDREQTVFYVKVLSTHLEQAV
                     EICADMLLHSTFAAESVERERQVITEEIKSYEDSPDELVQDLFAQTVWNGHPLGRPVI
                     GTLATISRLTRDDCLAHVQHYYRAANVVVAVAGEIEHERVVELMSRYFGAWDGAVDPA
                     AADPPIGLADVATRFKETEQVHLCLGVQGLAHDDERRYTLSVLDTLFGGGMSSRLFQE
                     IRERRGLVYTIGSYGASYRDGGLFVIYAGMSPSAGPEVITLTFDEIEKLKQEPPLPAE
                     LARAKESLKGSLMLGLESTGSRMTMLARSEVYYNRQITLDELIARIDAVTAGEVKQMA
                     IDLFHPSRLSMAAIGPFRPDGKLDGELKAAFRRQLGSAVTR"
     gene            4413..5219
                     /gene="dapB"
                     /locus_tag="AC159B_05715"
     CDS             4413..5219
                     /gene="dapB"
                     /locus_tag="AC159B_05715"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005808837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HIR3377275.1"
                     /translation="MGQRIRVAIAGAAGRMGRASVRTIAKQDDMSIVAALGRANQVGR
                     DAGEVAGGAPINVKITESLDEALASGVDVLVDFAPGAAAVAHARAAIASGVSPVIGGT
                     GMSSQEINELTGLSEARRVGAVVAPNFAVGAVLMMEFSRQAARYFPHAEIIELHHDRK
                     RDAPSGTAVKTASLIAAGRVGTPPSPVAEEETIAGARGGRAEGVRVHSVRLPGLVAHQ
                     EVIFGGSGQTLTIRHDSITEESFMPGLLLAVRKVRSLPGLVYGLEKLLEL"
     gene            5255..6283
                     /locus_tag="AC159B_05720"
     CDS             5255..6283
                     /locus_tag="AC159B_05720"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011434728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HIR3377276.1"
                     /translation="MMTDFRVGVVGATGAVGREVVKILRERRFPASSLRLIASGRSAG
                     MHLGGTVVEGISEEILRDLDVAIFDTPDDIARRWVPIASSLGVTSIDNSAAFRMEPDV
                     PLVIPEINAEALRNAPRRIIANPNCTTATIAVPLAPLHRVAGLTRVFACSYQAVSGAG
                     QKGVAQLWDELDESAKSHQPPGAARGDAFAHPIALNVIPAIGSFRGEHTSEEIKVGSE
                     LRKMLDAPDLRTGVTCVRVPTLVGHGVAVHAEFARPLDAATARRVLKDAPGVEVVDDP
                     AGSRYPTTLQSAGRDPCFVGRIREDGAGALAFFAIADNLRKGAALNTIQIAETMIEMG
                     LLAPRVRA"
     gene            6283..7527
                     /gene="dapG"
                     /locus_tag="AC159B_05725"
     CDS             6283..7527
                     /gene="dapG"
                     /locus_tag="AC159B_05725"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HIR3377277.1"
                     /translation="MQVVVQKYGGALLATADGREEAARQIIETRNAGSGVIAVASAIG
                     REGDPYATDTLITLLHEVDPRVDPRTMDLLLSTGEIISTALLAHMLVRLGCPAVALTG
                     AQAGILTTDEYTDARVLSIDASRIRRCLERGEVVVIAGFQGVTMDGEITTLGRGGSDT
                     TAVALGAALGAERVEIYKDVEGIMTADPKFVPAARPIETITYDEVSQMAALGAKVLHP
                     RAADLGREHDVRLVIKRLGSTNGGTLIVKGPELGVPINDGRPVIGLAHLPAVVQLKIR
                     RAAEKDLGRPMLALQTLAEHGISIDLINLLPEVLVFTVQEEAVLRAANLLSERGFDVA
                     VSEPCAKVSIIGAGMRGRPGVMARVVRTLHTAGVDILQTADSHTTISCLVQRGQMERA
                     LRALHDEFELAKEVASQRESEG"
     gene            7553..8443
                     /gene="dapA"
                     /locus_tag="AC159B_05730"
     CDS             7553..8443
                     /gene="dapA"
                     /locus_tag="AC159B_05730"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HIR3377278.1"
                     /translation="MSESFGRVLTAMATPFDESGAVDYGQVTALAKHLIANGSDGIVV
                     AGTTGESPTLSDDEKIRLFRTVKEAVGGRAAVVAGTGTYDTKHSIHLTKEAQRAGADG
                     VLLVNPYYNRPSQDGLYAHFRTVAESTSLPCMLYNIPSRTGVNCLPETIARLSEVPNI
                     TAVKEASGNLDQASEIRRLTMAEFRIYSGDDSLTLPILSVGGSGVVSVAAHLVGREIS
                     EMVKTYERGDISKALSIHIRLFPLFKVLFITTNPVPLKAALNLSRFNVGKPRLPLVEA
                     TAKEKEQIQSVLKELALAAV"
     gene            8474..9064
                     /locus_tag="AC159B_05735"
     CDS             8474..9064
                     /locus_tag="AC159B_05735"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR3377279.1"
                     /translation="MVVDKAKTLERLHSRILECTRCPLHLTRTQAVPGTGSADAEIVF
                     VGEAPGRQEDMQGTPFVGGAGRVFDGLLASIGLSRADVYITNTVKSRPFVGPPPGRNR
                     SPTPAEITACAPWLTEEMQLIRPKLLITLGQIALNQFLPRRKLSEVHGNPQRTDGVTI
                     LPLYHPAMARYGPEWTAILRADFLKISKVMTSQSRT"
     gene            9113..>9256
                     /locus_tag="AC159B_05740"
     CDS             9113..>9256
                     /locus_tag="AC159B_05740"
                     /inference="COORDINATES: protein motif:HMM:NF014703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIR3377280.1"
                     /translation="MVILGLVQGLTEFLPVSSTAHLIFTESFLGIPRPGILLEAVLHL
                     GTAL"
BASE COUNT         1810 a         2784 c         3008 g         1654 t
ORIGIN      
        1 acctcaggac gcccgccgac taggtaactc cctcacaggg gatctacgcc ggattcgctc
       61 gagtcccgtc gggtgcaggc tcgaccccgg gcagccaggc tgcgatcagc gtcgggacac
      121 gaccgacatt cggcccggga gagttactga tcgaggcgca tctcattgat gtctccgtgg
      181 acctgtacgg cgcccagatc gacgtgcaat tcgtggagcg cctccgcgat gaactgaagt
      241 ttgcttccat cgaagaactc atccggcaga tgcacgccga cgtcgagcag gcgcgcgtga
      301 tccttgtcac cccagaaatt cgctgacgcg aattccccgg ggaccccgac ctttcgcgcg
      361 catagcccgc atgataagat gcagagagtg aattggctgg gaggtccgtg cctccggcgg
      421 cccctcggcc ctgaaacttg cggttgttgg tcataggtcg tcggtcgtgg aaaagactcg
      481 atcttctgcc tacgacccac tacctacgac cgtagttggg atttatggag gtgaaggtgc
      541 gtggcccttc gcaaggcggc caaaacagag attgtgacga agtacaaacg ccatcaggaa
      601 gataccggct cgccggaagt ccagatcgcc ctacttacgg aacgcatcaa tcagttgacc
      661 gagcatctgg ggacccacaa gaaggatctc cactcgcgac gcgggttgct gaagatggta
      721 ggacagcgac ggggtttgct cacctacctc atgcgcagcg acccggctcg ataccgccag
      781 atcgtcgaca acctcggcct ccgccgataa attgcgtgaa aacacgatga ctccagtcga
      841 gcagaccccg gtgcaacgca ccgaggtaga cctcgccggc cgtacgttct cgctcgagac
      901 cggatggttg gccaagcagg ccaacggcag tgttgtggtc cgttacgggg aatcggtcgt
      961 gctggtgaca gcgacgatgt cggaaactct ccgtgagggc atcgatttct tccccctgac
     1021 gtgtgacttc gaggaaaaga tgtacgccgc cggcaagatc ccgggcgggt tcttcaagcg
     1081 ggaaggccgc cctggggagc gcgccatcct gtccggcagg ctcatggatc gcccgatccg
     1141 tccgctcttt ccaaaaggct ttcgcaatga cgtccaggtg attgccacgg tgctctcgac
     1201 agaccaggag aacgatccct cgattctcgc agtaaacggc gcgtccgcgg ccctgaccat
     1261 atccgacatc ccatttgatg gtccgatcgg agccgtccgc gttggactgg tcaacggtga
     1321 gcttgtcgtg aatccgacga tgaaacagat cgacgaggaa agcgatctcg atttcatcgt
     1381 cgccgcaagc gaggatgcgg tcgtcatggt cgaggcaggg gcgaacgagg tgaccgagga
     1441 gcgcctgctc gaagcgctcg atctcgccca ccgggagatc cgccgcatca tcggggcgca
     1501 gcgtgagctc gcctcgcgtg ccgggagggc caaggtttcg gtccagctcg ccacccccga
     1561 tcccgagctc gatgctgcgg tgcgtggcag ggcgctgcca ctcatcaaat cggcgctctc
     1621 gcaacccgag aagctgatgc gggaagacgc cctccggcag gtcgccgagc aggtggctcc
     1681 cgcgatcctg gaacagtatc ccgagcgcgc aaaggaaatt gaggaggtca ttgcgtcggc
     1741 gaccaaagag gaagtccggc ggatgattct cgacgagggg cgtcgcccgg atggtcgggg
     1801 cgtgcgcgac atccggccac tcaccgccac cgtcgggatc ctaccgcgtg cgcacggttc
     1861 cggactgttt cagcgcggac agacgcaggt gctctccgtc gccacgctgg gcacgggcga
     1921 ggacgagcaa atcattgatg acatcagcct gcgcaagagc aagcgcttca tgcaccatta
     1981 ctcgttcccg ccgttctcag ttggagaggt gcgacccctc cgcggcgcgg gtcgtcgcga
     2041 gatcgggcac ggactgctgg cagaacgagc gctcgagcgg atgattccag accagaccgt
     2101 gtttccgtac acgatccggc tcgtctcgga aatcctcgag tcgaacggat cgacctccat
     2161 gggttcggtg tgcgggtcga cgcttgcgtt gatggacgcc ggggtaccaa tcaaggctcc
     2221 agtcggcggg atctcgattg gactggtgac tgggcagcag ggcaagacgg cgttgctgac
     2281 cgacatccag gggatcgaag atgcgatggg ggacatggac ttcaaagtcg ccgggacacg
     2341 cgccggcgtc accgcattgc agctggacgt caagaacaaa ggattgtctc gagatgtgac
     2401 gcgccagacg tttcagcagg cgcgtgaagc tcgcctcacc atcctggacg ccatggcccg
     2461 ggcgatcgcc gcgccgcgcg gggaactctc tcgctacgcc ccacgcatca tcaccatcga
     2521 aatcaatcca gacaagatcc gggaagtcat cggccccggc ggaaaggtca tcaacaagat
     2581 cacggccgag accggcgtga agatcgatat cgagcaggat ggccgggtgc tcatcgcatc
     2641 ggtggatgag gaagccgcga gaaaagccag ggaaatgatc gaggccatcg tgcgcgaggc
     2701 gaaagtcggg gagacgtaca ccgggcgtgt cacacggctg atgaatttcg gagcgttcgt
     2761 cgaagtactg ccgggcaagg aaggactcgt gcacatctca gaactggcgg aggggcgccc
     2821 cgagagagtg gaagacgtcg tcaaagtcgg cgatgaactg accgtccggg tcaaagaaat
     2881 tgacagccaa ggccgcctca atctcacgcg acgcggtctc ccgggcggac ccaccggcga
     2941 ggccgctccc gtaccgtcag gtgaccggcg gccccgacgc gacgatcgac ggttcggcga
     3001 tcgccaccga cgacgagggc cgcgtcccga ttcccccaac caaccgtagc aaggcagcag
     3061 tagccccagg acttttctgc cacccgctta attccactgg agattatgag cgaactcatc
     3121 aataagacca cgcttcccaa cggcatccgc gtgctcaccg agcgaatgcc gcacgtccgc
     3181 acggcggcga tgggcatctg ggtcggcgca gggtcgcgct acgagcctgc gccgcagcac
     3241 gggatcacgc actttctgga gcatctcttt ttcaaaggta ccgagaagcg gagcgctctc
     3301 gagatcgccc agaccgcgga tgatatgggc ggccagatga atgcatttac cgatcgcgag
     3361 cagaccgtgt tctacgtgaa ggtcctgtcc acgcacctcg agcaggcggt ggagatttgc
     3421 gccgacatgc tcttgcacag caccttcgct gcagagtcag ttgagcgcga gcgtcaggtc
     3481 atcactgaag agatcaaaag ctacgaagat tccccggacg agctcgtgca ggacctgttt
     3541 gcgcagaccg tctggaacgg acaccccctc gggcggccgg taatcgggac cctggcaacc
     3601 atcagtcgcc tgacgcgcga cgattgtctg gcgcacgtgc agcattatta ccgcgcggcc
     3661 aacgtcgttg tggccgtggc aggcgagatc gagcacgagc gcgtcgtcga gttgatgagc
     3721 cggtacttcg gcgcgtggga cggggcggta gatccggcgg cggccgatcc cccgatcgga
     3781 ctcgccgatg tcgcgacacg attcaaggag accgagcagg tgcatctgtg tctcggcgtt
     3841 caaggcctgg cacatgacga cgagcgccgc tacacgctgt cggtcctgga cacgctcttt
     3901 ggcgggggca tgagctcgcg gttattccag gagatccgtg aacggcgcgg gctggtgtat
     3961 acgatcggtt cgtacggcgc ttcgtaccgg gacggcgggc tgtttgtgat ctacgccggc
     4021 atgagtccgt cggccggccc ggaggtgatc acgctcacct tcgacgagat cgaaaaactc
     4081 aagcaagagc cgccgttgcc cgcggagctg gccagagcaa aggaatccct gaagggaagt
     4141 ttgatgctgg ggctggagag caccggcagc cggatgacga tgctggcgcg atcggaagtg
     4201 tactacaatc gccagatcac gctcgacgaa ctcatcgcgc gcatcgacgc ggtcaccgcg
     4261 ggcgaagtga agcagatggc gatcgacctc tttcacccca gccggctgtc gatggccgcc
     4321 attgggccat tcagacccga cggtaagctc gacggcgaat tgaaagctgc attcaggcgt
     4381 cagctgggta gcgcggtaac gcgttaaggc ggatgggtca aaggatcaga gtcgctatcg
     4441 cgggagcggc cggccggatg ggacgcgcgt cggtgcggac catcgccaag caagacgaca
     4501 tgagcatcgt cgcggcactc gggcgcgcga atcaggttgg ccgcgacgcc ggtgaggtgg
     4561 cgggtggagc cccgatcaac gtcaagatca ccgagagtct ggacgaagcg ctcgcatcag
     4621 gggtggacgt cctcgtggat tttgcgcccg gcgcggccgc ggtcgctcat gcacgcgccg
     4681 cgatcgcttc gggcgtcagt cccgtaatcg gcgggaccgg gatgagcagt caggagatca
     4741 atgaattaac gggtctgagt gaggcgcgtc gggttggcgc ggtggtcgcc cctaactttg
     4801 cggtcggcgc agtcctgatg atggagttca gcaggcaggc cgccaggtat tttccgcacg
     4861 cagaaattat cgagctccat catgaccgga aacgcgacgc gccgtccggg acggcggtga
     4921 aaaccgcatc gctgattgcc gcgggcaggg tcgggacgcc gccctcgccg gtcgccgagg
     4981 aagaaacgat cgccggtgca cgagggggcc gtgccgaagg cgtgcgcgtg cacagcgttc
     5041 gtcttccggg gctggtggcg catcaagagg tcatttttgg agggtcgggg cagacgctga
     5101 cgatccggca cgactccatt accgaggagt cgttcatgcc ggggctgttg ctggcggtgc
     5161 ggaaggtgcg ttcgctgccc gggctcgtct acgggctgga gaaacttctt gaactatagc
     5221 tgaagagctg gaaggaaaat gaggtacata taggatgatg acggattttc gtgttggggt
     5281 cgttggggcc acaggtgccg tcggtcgaga ggtcgtgaag atcctccgcg agcggcgctt
     5341 tccggcctcg tcgctgcggc tgattgcctc cggacggtcg gccgggatgc atcttggcgg
     5401 cacggtcgtt gagggaatct ccgaggagat tctcagagat ttggatgttg cgattttcga
     5461 cacgccggac gatatcgcaa ggcgatgggt accgattgcc tcgtccctcg gggtcacctc
     5521 catcgacaac tccgcagcgt ttcgcatgga acccgacgtg ccgctggtga ttccagagat
     5581 caacgccgag gcgctgcgga atgctccacg gcgcattatc gccaacccga attgtacgac
     5641 cgcaacgatc gccgttcccc tcgcgcccct ccatcgtgtg gcgggactca cccgagtctt
     5701 cgcgtgctcg taccaggccg tttcgggggc cgggcagaag ggcgtggcgc agttgtggga
     5761 tgaactggac gaatccgcca agtcgcatca gccgcccgga gcggcgcgtg gcgatgcatt
     5821 cgctcatcca atcgcgttga acgtcatccc cgcgattggc tcattcaggg gtgagcacac
     5881 aagtgaagaa atcaaagtgg gctccgaact ccgcaagatg ctcgacgcgc ctgacctccg
     5941 caccggtgtc acctgtgtgc gtgtcccaac gctcgtcggc catggcgtcg cggtacacgc
     6001 cgaattcgcc cgaccactcg atgctgcgac ggcccggcgg gtgttgaagg acgcgcccgg
     6061 cgtcgaggtg gttgatgatc ccgcagggag tcgctacccg acgacgctgc agtctgccgg
     6121 ccgggatccc tgctttgtgg gacggatccg cgaagacggg gcaggagcgc tggcgttctt
     6181 tgcgatcgcc gacaatctcc gaaaaggcgc ggcgctcaac accattcaaa tcgctgagac
     6241 catgatcgag atgggattgc tcgcgccgag ggtccgcgct tgatgcaagt ggtcgttcaa
     6301 aagtacggcg gtgctctgct cgcgacagct gatgggcgcg aagaggctgc ccggcagatc
     6361 atcgagacgc gcaacgccgg atcgggtgtc attgcggtcg cttcggcgat tggccgtgaa
     6421 ggggatccat acgcgaccga cacgttgatc acgcttctcc atgaggttga cccgcgggtc
     6481 gatccccgga ccatggatct gttgctctcc actggggaga ttatctcgac cgcgctgctg
     6541 gcccatatgc tcgttcgcct cggatgtccg gccgtggccc tcacgggcgc tcaggccggt
     6601 attctgacaa ccgacgaata caccgatgct cgtgtcctca gcatcgatgc ctcgcgtatc
     6661 cgccgttgcc tcgagcgggg tgaagtggtc gtgatcgcgg gttttcaggg tgtgacgatg
     6721 gatggcgaga ttacgacgct gggtcgaggc ggcagtgaca ccacggccgt ggcgctcggc
     6781 gccgccctgg gcgccgagcg cgtggagatc tacaaggacg tcgaaggcat tatgacggcc
     6841 gacccgaaat tcgtgcctgc cgcccgcccc atcgagacca tcacatatga cgaggtctca
     6901 caaatggcgg ccctgggcgc caaagtgctg cacccgcgag ccgcagatct cggacgggag
     6961 catgacgtcc ggctcgtcat caagcggctc ggcagtacaa acggagggac actcatcgtg
     7021 aagggaccgg agctgggagt gccgatcaac gatgggcggc cggtgatcgg cctcgcgcac
     7081 ctgccggcgg ttgtacagtt gaagatccgg cgggcggctg aaaaggatct cggcaggccg
     7141 atgctcgccc tccagacgct ggcggagcac ggcatcagca tcgacctcat caacctcctg
     7201 ccagaggttc tcgtgtttac cgtgcaggag gaggcggttt tgcgtgccgc taaccttctg
     7261 agcgaacgcg gttttgatgt cgcggtaagc gagccgtgcg cgaaagtgtc gatcatcgga
     7321 gccggcatgc gaggacgacc cggggtcatg gcgcgcgtcg taaggacgct gcacaccgcc
     7381 ggcgtggaca tccttcagac ggcggactcg cacacgacga tttcgtgtct ggtacagcgg
     7441 ggacagatgg aacgcgcgtt gcgcgcgttg cacgatgagt tcgagctggc gaaggaagtc
     7501 gcatcacagc gagagagtga agggtagacg ccgaatttga gaggtgagtg ggatgtctga
     7561 gagcttcggg cgcgtgctga ccgcgatggc gaccccgttc gacgaaagcg gggccgtgga
     7621 ttatgggcag gtgacggccc tggcgaaaca cctgatcgcg aacggatcag acggaatcgt
     7681 cgtcgccgga accactggag aatcgccgac gctgagcgat gacgagaaga tacgcctgtt
     7741 ccgcacggtg aaggaagcgg tcggcggccg ggctgcggtc gtggccggca ccggcaccta
     7801 tgacaccaag cactcgatcc acctcacgaa agaagcgcag cgcgccggcg cggatggcgt
     7861 attgctggtc aatccctatt acaaccggcc gagccaggac ggtttgtatg cgcacttccg
     7921 cacggtcgcc gagtcgacat ctttgccgtg catgctctac aacatcccca gtcgcaccgg
     7981 ggtcaactgc cttcctgaaa caattgcgag gctgtccgag gttccaaaca tcacggcggt
     8041 caaggaggcc agtggaaacc tggaccaggc gtcggagatt cgacgcctca caatggccga
     8101 gttccgcatc tacagcggcg acgacagcct cacgctgccg atcctctcgg tcggcggcag
     8161 cggcgtcgtg agtgtggccg cgcatctggt tggccgcgag atttccgaga tggtgaaaac
     8221 atacgagcgt ggtgacatca gcaaggcgtt gtccatccac atccggctct tcccgttgtt
     8281 caaggtcctc tttattacca ccaatcctgt accgctcaag gcggcgctga acctgagcag
     8341 gttcaacgtc ggcaagccaa ggctgccgct ggtcgaagcg acggcgaagg agaaagagca
     8401 gattcagagc gtgctcaagg agctggcact cgccgccgtc taacactcga ttacggtcag
     8461 gggtcgtcgg tcgttggtcg tagataaggc caaaactctc gaacgacttc actcgaggat
     8521 ccttgagtgc acccggtgcc ctcttcacct cactcgcacg caggccgtcc ccggtacggg
     8581 atcagcggat gcggagatcg tcttcgtcgg ggaagcgccg ggacgccagg aagacatgca
     8641 gggtacgccc ttcgtcggcg gcgccgggag agtgttcgat gggctgctgg cctcaatcgg
     8701 gttgtcccgg gccgacgtat acatcactaa taccgtcaag tctcgtccgt tcgtgggtcc
     8761 accaccggga cgtaatcgat cgcccacgcc cgcagaaatc acggcatgcg ctccgtggct
     8821 cactgaggaa atgcagctca tccggcctaa gcttctcatc acgcttgggc aaatcgccct
     8881 caaccagttt cttccacgtc gaaagctctc tgaggtccac gggaatcccc aacgcaccga
     8941 cggcgtgaca attcttcctt tatatcaccc tgccatggcg cgctacgggc ccgagtggac
     9001 tgccattttg agggcggatt tcctcaagat ttcgaaggtc atgacctccc agtctcgaac
     9061 gtagtttcgt tactgacaac ggacctttca acggagcgac aatgtgcttg agatggtcat
     9121 cctcggcctg gtgcagggcc tgaccgagtt cctgccggtc agcagcactg cacacctcat
     9181 tttcaccgag tccttcctcg gcatcccgcg gccagggatc cttcttgagg ccgtcctcca
     9241 tctcggaacg gcgctc
//
LOCUS       DBNGSB010000196         3582 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_1521522, whole genome shotgun sequence.
ACCESSION   DBNGSB010000196 DBNGSB010000000
VERSION     DBNGSB010000196.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_1521522"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="AC159B_05745"
     CDS             <1..421
                     /locus_tag="AC159B_05745"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3377281.1"
                     /translation="LRETLGVVRVGDIMSRKIHSVDPNVTLEQLIADYFLPYKHGGFP
                     VMWGNRLVGIITLHDVKEVPKEKRATMTVRDAMTPTSRLRTVKPITSAYDAFARMAQE
                     GIGRLLVVDDGGDLVGILTRSDLLHVLRIRTELEDLA"
     gene            complement(418..945)
                     /locus_tag="AC159B_05750"
     CDS             complement(418..945)
                     /locus_tag="AC159B_05750"
                     /inference="COORDINATES: protein motif:HMM:NF021492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3377282.1"
                     /translation="MGEAEARSFLEEGEGHLQSGRYEAAERAFTKAVEAAPDSAVAHS
                     KLGVALANQHRYDAAISHFNQAVALQPGYAPAYSNMGNAYREKGMPNEAVMAYERALA
                     IDPDYWVAHQNLGALYKEMGRMGEAVQHFKKAMRLSARRTTKPGERERRVGCLGPGTV
                     AVLFLLMSLALALAR"
     gene            complement(985..1461)
                     /locus_tag="AC159B_05755"
     CDS             complement(985..1461)
                     /locus_tag="AC159B_05755"
                     /inference="COORDINATES: protein motif:HMM:NF024848.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase-like protein"
                     /protein_id="HIR3377283.1"
                     /translation="MILDLYTSTAARDGRVAAAAVIRDAEGRTLRVTARSLEGTSREE
                     AAYRALLHGLWRARRMGARRVRVYCGDATVLAQLEGEIEVPVELVGVYLQTRAMLNAY
                     RWSSVELVQRDRNAEASLASVDALDREPDSRGFEIEEIEAMPLWEQAGRERVGTSR"
     gene            complement(1472..1699)
                     /locus_tag="AC159B_05760"
     CDS             complement(1472..1699)
                     /locus_tag="AC159B_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3377284.1"
                     /translation="MSVRAIAVQDRKRHRMRVVARPLDPPRVIRRRHRWVNTAEIEQV
                     RGFRDDERLVRLKLIMLGGIVFLAALLETPF"
     gene            complement(1696..2325)
                     /gene="lexA"
                     /locus_tag="AC159B_05765"
     CDS             complement(1696..2325)
                     /gene="lexA"
                     /locus_tag="AC159B_05765"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HIR3377285.1"
                     /translation="MTPLTDRQRQVLEHIATSIRRSGIVPSVREIGHALGMRSPSTVH
                     QHLTALARKGYIKRYGDRMRVLEVLDRRILPDSDDVIHVPLVGRVSAGVPILAQENIE
                     EMIPVPRKMVGWHDENCFLLTVRGDSMIHAGIFDGDIVIVRSQPQADPGEIVVALLGE
                     EATVKRLAVDGRRPYLLPENPRYGPIYDEFEVIGKVVGLMRRYAGGSPA"
     gene            complement(2491..3423)
                     /locus_tag="AC159B_05770"
     CDS             complement(2491..3423)
                     /locus_tag="AC159B_05770"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007469296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HIR3377286.1"
                     /translation="METTALTISLADIYAARERIAPYVHRTPLLPMPGGIYLKCENLQ
                     VPGAFKARGAFNHVLALRHECERGIVTASSGNHGNAVAHVAKTLGLRAVVVVPEDVVA
                     AKADAITDSGAELIRFGRYSEQRVPHAKEISERRGLHYVPPYDDPLIIAGQGTAGLEL
                     VEQFPHVETVAVPVSGGGLISGIAIAVKELRPQAKVIGVEPERARRFAQSREAGRPVV
                     LELAETIADGLRVLTPGALTWQVTSRYVDEFVGISDHEIETAMRRILLDAHVLAEPSG
                     AAAAAWTFLAPHPSLVAVLSGGNASPDLLSRLLA"
     gene            complement(3405..>3582)
                     /locus_tag="AC159B_05775"
     CDS             complement(3405..>3582)
                     /locus_tag="AC159B_05775"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020154082.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HIR3377287.1"
                     /translation="ETESKRTMDEFIEALLAITREAKEDPELVRTAPHTTPVKRLDEV
                     RAARHPDLRWKPRR"
BASE COUNT          674 a         1138 c         1090 g          680 t
ORIGIN      
        1 gctccgcgag accctcggcg tcgtgcgcgt cggcgacatt atgagccgga agattcattc
       61 cgtggatccg aacgtgacgc tcgagcagtt gatcgccgac tacttcctcc cttataagca
      121 cggtgggttc ccggtgatgt ggggcaaccg cctcgtcggc atcatcacgc tgcatgacgt
      181 caaggaagtt ccgaaggaga aacgggcgac gatgacagtg cgcgatgcca tgactccgac
      241 gtcgcgcctc cgcacggtga aacctatcac ctcggcgtac gatgcatttg cccggatggc
      301 ccaggagggc atcggcaggc tattggtcgt tgacgatggc ggcgatttgg tgggtattct
      361 gacccgaagc gacctgctgc acgttctgcg gatcaggacc gaactggaag acctggccta
      421 gcgcgcgagt gcgagggcga gagacatcag cagaaacagc acggcgaccg tgccgggccc
      481 gagacagccg acccggcgtt cacgctcacc aggttttgtt gtccgccgag cggacaggcg
      541 catcgctttc ttgaagtgct gcaccgcctc gcccattcga cccatctctt tgtacagcgc
      601 gccgaggttc tgatgcgcga cccagtagtc ggggtcgatg gccagcgccc gttcatacgc
      661 catgacggct tcgttgggca tgcctttttc tcgataggca ttgcccatat tgctgtacgc
      721 gggcgcgtag ccgggttgaa gtgcgacggc ctggttgaag tgagagatgg ctgcgtcgta
      781 gcggtgctga ttcgcaagcg caaccccgag tttgctatgc gcgaccgccg agtccggggc
      841 agcttcgaca gccttggtga atgcgcgttc cgcagcttcg tagcgaccgc tctgaaggtg
      901 accctcccct tcttcgagaa atgacctcgc ctccgcttcg cccacaggga aagactaaca
      961 aaaaagaagt gccaaacctc ggaattagcg gctcgtcccg accctctccc tccctgcctg
     1021 ctcccacagc ggcatcgcct cgatctcttc aatttcaaac cctcgagaat cgggttcccg
     1081 atcgagcgcg tcgaccgacg ccagcgacgc ttcagcgttt cggtcccgtt gcaccagctc
     1141 gacactgctc caccggtagg cgttcagcat cgccctggtt tgaaggtaca cgccgaccaa
     1201 ctcaaccggg acttctatct ccccttcgag ctgcgcgagg acggtcgcat cgccgcagta
     1261 gacccgcacg cgccgcgcgc ccatgcgccg cgcgcgccac aatccgtgca gcaacgcgcg
     1321 atatgccgcc tcttcacgag aggtgccttc cagcgaacgc gccgtaaccc gaagcgtacg
     1381 tccctccgcg tctcggataa cagcggccgc ggcgacgcgt ccatctcgcg ctgcggtgct
     1441 tgtgtaaagg tcaagtatca taccgaagat attagaacgg cgtctccagg agagccgcca
     1501 ggaaaacgat ccctcccagc atgatcagtt tgagccgcac caggcgctcg tcatcgcgaa
     1561 acccacgcac ctgttctatc tcggcagtgt ttacccagcg atgccggcgg cgtatcacgc
     1621 gcggaggatc aagcgggcgc gcgacgacac gcatacggtg gcgtttgcga tcttgaaccg
     1681 caatagcgcg gacgctcatg ccgggctccc tcccgcgtag cgccgcatca gtccgacgac
     1741 ttttccgatg acttcaaact cgtcgtagat cggcccatag cgcggatttt ctggaagtaa
     1801 atacggtcgg cgcccatcga ccgcgagtcg ctttacggtg gcttcctcac ccagtagcgc
     1861 aaccacaatt tccccaggat cggcctgtgg ctgggagcgg acgatcacaa tatcgccgtc
     1921 aaagatgccg gcgtgaatca tgctgtcccc gcgaacggtc agaaggaagc aattctcatc
     1981 gtgccagccc accatcttgc gaggcacggg aatcatttcc tcgatgttct cctgcgccag
     2041 gatcggcacg cccgcggaga cacgcccgac gagcggcacg tgaatcacgt catcgctgtc
     2101 ggggaggatg cgccggtcga ggacctccag gacgcgcatg cggtcaccgt agcgcttgat
     2161 gtaccctttc cgcgccagag cggtcaggtg ttgatggaca gtggaaggag agcgcatgcc
     2221 gagcgcgtgg ccgatctccc ggaccgatgg aacgatgcca ctccggcgga tggacgttgc
     2281 gatgtgttcg aggacctgac gctgccgatc tgtgagcgga gtcatgggta cacctccgcc
     2341 ctaattgtat ggcgaacaaa cgtacgaaca tcaactgctg gggaatatga aggatcacca
     2401 cgtgaaccga acgtttgtgc gtatgacccc agcgctcgta atatctcgtt attgctcgcg
     2461 atttcgcgta attgcgcctt cgcgcgtgag ttaagccagc aaacggctca gcaggtcggg
     2521 gcttgcgtta cctccactca gcacggccac gagggagggg tgcggagcga ggaaggtcca
     2581 cgcggccgcg gcggcaccag acggctccgc gagcacgtgg gcgtcgagca gaattctgcg
     2641 catggcggtc tcgatctcgt ggtcggagat gcccacgaac tcgtcgacat aacgcgacgt
     2701 cacctgccac gtcaaagcgc cgggtgtcag gacgcgcagc ccgtcggcga tcgtctcggc
     2761 taactcgaga accaccggcc tgccggcctc gcgtgattgc gcaaaccgcc tggcacgctc
     2821 gggttcgacc ccgatgacct ttgcctgtgg ccgtagttcc ttcaccgcga tcgcaatacc
     2881 cgagatcagc cccccaccac ttacggggac ggccacggtc tccacgtgcg ggaattgctc
     2941 gacgagctcg agcccggcgg tcccttggcc ggcgatgatc agcgggtcgt cgtagggagg
     3001 aacatagtgc aggccgcggc gttcgcttat ttctttggca tgagggacac gctgctcgga
     3061 ataccggccg aaccgaatga gctccgcgcc tgagtcggtg atggcatcgg cttttgccgc
     3121 gacgacatct tcaggaacga ccacaacagc ccggagtcca agcgtttttg ccacgtgtgc
     3181 gaccgcgtta ccgtggttac ccgaggaggc ggtgacgatc ccgcgctcgc attcatggcg
     3241 cagcgcgagc acgtgattga acgccccgcg ggctttgaat gcacccggga cttgcaggtt
     3301 ctcgcacttg aggtagatcc cgccaggcat ggggagcagg ggagtgcggt gcacgtacgg
     3361 cgcgatacgc tcgcgcgcag cgtagatatc tgcgaggctg attgttaacg ccgtggtttc
     3421 caacgcagat ccggatggcg tgctgcccgg acttcatcga gacgcttcac cggtgtcgtg
     3481 tgcggcgcgg tccgcacgag ttctgggtcc tctttggcct cgcgggtaat cgccagaagc
     3541 gcctcgatga actcatccat agtacgcttg ctctccgtct cc
//
LOCUS       DBNGSB010000197         6319 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_2442378, whole genome shotgun sequence.
ACCESSION   DBNGSB010000197 DBNGSB010000000
VERSION     DBNGSB010000197.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 6319)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6319)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6319
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_2442378"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="AC159B_05780"
     CDS             <1..979
                     /locus_tag="AC159B_05780"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3377288.1"
                     /translation="SRIYLAIKSYPSDFGLAGSYSTILLVMTLTGIFLYGRITREASR
                     FATITGKGFRPHVIDLGIWKYAMAALIVGYLVVMVVLPMFVLVWNSVLPFYMRPSFPA
                     LSMLTIDNYTFVLKLPKTTRAVINSIGLSMGSATIVVIMTSVIAWITVRSRLPGRWLL
                     DNLASLPLIFPGIVMGVALIWVYLTLPIPIYGTVWILLVAYVTRYLPYGMRTSSAALL
                     QVHRELEEAAETSGASWGRTFRSIILPLLQPALLAGWIYVVIVSIRELSSSILLWGPG
                     AEVLSVYIFDLWQTGHPQELSALGLMLVGFLLVLAMVLRALSQRYGVQH"
     gene            982..2097
                     /locus_tag="AC159B_05785"
     CDS             982..2097
                     /locus_tag="AC159B_05785"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3377289.1"
                     /translation="MVKVQDLVKVYRTGRGSVVACDRVSFEVEDGRFFTLLGPSGSGK
                     TTTLRCVAGLERPDGGQIELAGTVVSSNQHAIFIPPNRRDIGMVFQSYAIWPHMTVFD
                     NVAFPLRAGGHRPSSGEVMRRVSQALATVRLEGLQDRPATKLSGGQQQRLALARALVR
                     EPTLLLLDEPLSNLDAKLREQMRLELRQLQRELRITTLYVTHDQIEALAMSNVVAVMH
                     EGRIIQVGTPREIYERPANRFVAEFIGSMNLIPGRVAGSVEGGLSRVETPYGPIACVV
                     PPDGRPASDVLVSVRPEDIVVTDDESAGKDAWDASIGQVVFLGESVDCRLAVGDLELR
                     ARLHPSFVKRRGEKVRLSFRADRCVAIRLEPPETETA"
     gene            2194..3423
                     /locus_tag="AC159B_05790"
     CDS             2194..3423
                     /locus_tag="AC159B_05790"
                     /inference="COORDINATES: protein motif:HMM:NF024241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3377290.1"
                     /translation="MVVGSLWQRVPRPDRANAGMWAIGLGHAMTHVYTAALYILLPFL
                     ARDLGLTYSQIGLMLAVRQFMSAIVNLPAGIIVDTVGRRSLLMGLSLLGVSVPYLIIS
                     ATSQYWVIVLCFGLMGIATFLWHPAAITSISEMYPARRGYGLAIHELGSNLGDTVTPL
                     LAGALLGFLTWRQVISVTLAAGILVGIIVLRTITRIRRQYIEPAASAAERTGYLGGLR
                     ILVRNTSLLILALVSGIRSFTQHGLISFLPLYLVNDLKVPAILVGVYLAVVQVSGMIA
                     TPISGTLTDRIGPKRVATTGMLASSLVVLAFAALDLGSAFVLALALVGFFIYSMRPAI
                     FRWAIGVVPRQYEGTTVGALFTTQAMFSTMMPLVGGVLADRFGLISVFYVIAIGLVIA
                     NLITLMVPDLRRPGAAN"
     gene            complement(3373..5703)
                     /locus_tag="AC159B_05795"
     CDS             complement(3373..5703)
                     /locus_tag="AC159B_05795"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3377291.1"
                     /translation="MPRIHWIGRPIKRREDERFLRGRGRYIDDLTLPGMLHMVVVRSS
                     QAHAKLRRIRTDEAMAIPGVVAIVTAQDLIGKVRSMPLRAEEGMTIADIPHPLLATDK
                     VRYTGEPVAAVLAQTRAAALDAAATIEVDYDPLPEVVDVSDALNGTTLVHEAVGENVV
                     VRWEHTGGDVDGAFSRADRVVNGKFRIPRLVAVPIETRGVVASYDRETDLLTVWCSAQ
                     DPYRPRTHLSHVLNRSQDRIRVIVQDVGGAFGTKGSLAPEQALAAFLAVSTERPVKWV
                     EDRRENFLAAYQGRGVEADVQMAVTRDGKILAVKGRIVGDLGAYLYAPTASRPIITAM
                     MLTGAYEIPSAQVDAVGLTTNKVPIGPYRGAGRPEAAYIVERMLDLVARELALDPVEV
                     RRGNLIPSNRFPYKTPTGLVYDSGNYERALDRALELLDYDGWKVRQREARAAGRLLGI
                     GVAVYVERAAPAVWERTDVRVDPDGLVRVRPGSSSHGQGHETIFAQIAADNLQLDLDA
                     VTVEFGDTARMPEGVGTFGSRSTTLGGSALILALDEVRAKAIKIAAHLLEAAEGDIEL
                     TEGHFAVRGTIGRSVSFDDVAAASYRLENLPSGMDPGLEASGEFRMPDPVFPFGAYAA
                     VVEVEHETGQVMIHRFVAVDDAGRIINPLLAEGQVIGATVQGLGQALVEEAVYDDSGQ
                     MLTGSLTEYGILHAPQVPEVHSELLETRSPLNPLGAKGIGEAGTIGAPPAIVNAVVDA
                     LEPFGVRHIDVPLRPEKIWRLIRSARTPEVGDHQRD"
     gene            5770..>6319
                     /locus_tag="AC159B_05800"
     CDS             5770..>6319
                     /locus_tag="AC159B_05800"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3377292.1"
                     /translation="MQKISSVGILGLGRMGLPMGRHLLRAGFAVHGFDPHEAQLAALS
                     DAGGKALRSASAVAGKSEAILIMVADDAQVKEVTMGSGGVFEAAKPGTVCIISSTVKP
                     STCREIATAAEKRGIKVLDAPVARGQRAAEEGTLTVFAGGERSTFEQCRPIFEAFGKE
                     VVHIDERVGSGQVAKLVNNLILW"
BASE COUNT         1118 a         1927 c         1942 g         1332 t
ORIGIN      
        1 ctccaggatc tatcttgcga tcaagagcta tccctcagac ttcggcctgg cgggatcata
       61 ctcgacgatt ctgctggtga tgacattgac cggcatcttc ttgtatggcc ggattacacg
      121 agaggcatcg cgatttgcca cgatcaccgg caaagggttt aggccgcatg tcatcgatct
      181 agggatctgg aaatatgcca tggcggcgtt gatcgttggg tatctcgtgg ttatggtggt
      241 cctgccgatg ttcgtgcttg tgtggaactc cgtccttccg ttttacatga ggccgtcctt
      301 tccggcgctc tcaatgctga cgattgacaa ctacacattt gtgctcaaac tgcccaaaac
      361 gacgcgggcg gtcatcaaca gcattgggct gtctatggga agcgcgacca ttgtcgtgat
      421 catgacctcg gtcatcgcgt ggatcaccgt acgctctcgt ctgccaggcc ggtggctgct
      481 cgataacctg gcgtcgttgc cgcttatctt tcccggcatc gtcatgggcg tggctctgat
      541 ctgggtatac ttgacgctgc cgattccgat ctacggaaca gtatggattt tgctggtcgc
      601 ctacgttacc cggtatctgc cctatgggat gcgcacatca tctgccgcgc tgctgcaggt
      661 gcaccgggaa ttagaagaag cagcggagac cagcggagcg tcgtggggac ggacgttccg
      721 gagtattatt ctgccgctgc tgcagccggc gctgctggcg ggctggatct atgtcgttat
      781 cgtgtccatc cgggagctct cgagcagcat tctgctgtgg ggtccggggg cagaggtgct
      841 gtccgtctac atctttgatc tctggcagac cggccaccca caggaactct cggcgttggg
      901 actgatgctt gtaggttttc tcctcgtcct tgccatggtg ttgcgggcgt tgagccagcg
      961 atatggagtg cagcactagc catggtcaag gtccaggatc ttgtgaaagt gtatcggacc
     1021 ggacgtgggt cggtcgtggc ctgcgaccgt gtcagttttg aagtcgagga cggccggttc
     1081 ttcacgctct tggggccatc gggatccggc aagacgacga cgctgcgctg cgttgccgga
     1141 cttgagcgac ctgacggagg acagatcgag ctcgccggta cggtggtgtc ctccaaccaa
     1201 cacgccatct tcatcccgcc gaaccggcga gacatcggca tggtgttcca gtcgtacgcg
     1261 atctggccgc acatgaccgt gttcgataac gttgcgttcc ctcttcgcgc ggggggccac
     1321 cggccatcat caggtgaggt gatgcggcgt gtgtcgcagg cgctcgcgac cgtgcgcctg
     1381 gaaggattac aggatcgccc tgccaccaaa ctaagcggcg gccaacagca gcggcttgcg
     1441 cttgcccgag cgctcgtgcg ggagcccacg ttgctgctgc tggacgagcc gctctccaac
     1501 ctcgatgcca aactgcgcga acaaatgcgt ctggaattga gacagctgca gcgggagctc
     1561 cgcatcacta ccctgtacgt cacgcatgat cagatcgaag cgctggcgat gagcaacgtc
     1621 gttgccgtca tgcacgaggg ccggatcatc caggtcggaa ccccgcggga gatctacgag
     1681 cgtcctgcga accgcttcgt ggccgagttt atcggctcga tgaatctcat accgggaaga
     1741 gtggccggtt ccgtcgaggg tgggctgtcg cgagtagaga ccccctacgg ccccatcgcc
     1801 tgcgtcgtgc cgccggacgg ccgtcccgcg tcggacgtac tcgtctcggt tcgcccggag
     1861 gacatcgtcg tgaccgatga cgagagcgcc ggcaaagacg cgtgggacgc gtcgatcggg
     1921 caggtggtgt tcctgggtga atcggtagac tgccgcctcg ccgtcggtga cctcgagctg
     1981 cgcgcgcggc tgcacccgtc gttcgtgaaa cgacggggtg agaaagtgcg gctgtcgttc
     2041 cgcgccgacc gctgcgtcgc catccgcctg gagcctcccg aaaccgagac cgcgtgaaca
     2101 cgacatcact caacccggca ccacgcaaaa ttctcccggc ctcgacctag ctcggcctgt
     2161 gtgcgtcggc ggcccactgc gtgggcgatt ctagtggtcg ttggaagcct gtggcagcgc
     2221 gtgccacggc ccgatcgcgc caacgcaggt atgtgggcga ttgggctggg gcacgcaatg
     2281 acgcatgtct acacagcggc cctgtacatc ctgctgccgt tcctcgcgag ggacctgggt
     2341 ctcacctaca gccagatcgg cctcatgctc gccgtccgcc agttcatgag cgcgatcgtc
     2401 aatcttccgg ccggcattat cgtggacacc gttgggcggc gcagcctgct catggggcta
     2461 tcgctcctgg gcgtctcagt accgtacttg atcatcagcg cgacctcgca atactgggtc
     2521 atcgtgctat gttttggact catgggcatt gccacatttc tctggcatcc cgccgccatc
     2581 acatccatct ccgagatgta tccggcccgc cgcggatacg ggctggccat tcatgagctg
     2641 ggctccaatt tgggcgacac ggtgactccg ctattggccg gagcgctgct gggtttcctg
     2701 acatggcggc aggtcatttc ggtcacactc gcggcaggga tcctggtcgg gatcattgtt
     2761 ttgcggacaa ttactcgaat acgccgccag tacatcgaac cggcggcgag tgctgccgag
     2821 aggacgggat accttggggg attgcgcatt ctcgtgcgca atacaagtct gctgatcctc
     2881 gcactcgtat ccggcattcg ctcgtttacg cagcatggac tgatcagctt tctccctctg
     2941 tatcttgtga acgatttgaa ggttccggcc attctcgtcg gcgtttacct cgccgtcgtg
     3001 caggtctctg ggatgatcgc caccccgatt tcaggcacgt tgacggaccg cattggaccc
     3061 aagcgtgttg ctaccacggg gatgcttgcg agcagtctgg tggtgttggc gtttgcggcg
     3121 ttggatttgg gatctgcgtt tgtgctcgcg ttggcgctgg tcggattctt catctactcg
     3181 atgcgtccgg cgatttttcg gtgggcgatt ggggttgtcc cgcgacagta cgaggggacg
     3241 acagtgggcg cgctttttac gacgcaggca atgttctcga caatgatgcc gctcgtaggt
     3301 ggcgttctcg cggaccgttt cggactcatc tccgtcttct acgtaatcgc catcggtctc
     3361 gtcatcgcca acctaatcac gttgatggtc cccgacctca ggcgtccggg cgctgcgaat
     3421 tagtctccat attttctccg gccgcagggg cacgtcgatg tgccgcacgc cgaacggttc
     3481 gagtgcatca accaccgcat tgacgatcgc agggggcgcg ccgatggttc ccgcttcgcc
     3541 gatgcctttc gcgcccagcg gattcagcgg tgaacgggtc tccagcagct cactgtgcac
     3601 ctcgggtacc tgcggcgcat gcaagatccc gtactcggtg agcgagccgg tcagcatctg
     3661 gccactgtcg tcatagaccg cctcctcgac gagcgcctgc ccgagaccct gcacggtcgc
     3721 cccgatcacc tggccctcgg cgagcaaggg attgatgatc cggcccgcat cgtccaccgc
     3781 gacaaaccgg tgaatcatga cctgtccggt ctcatgctcc acctcgacga cagccgcgta
     3841 cgcgccgaac ggaaatacag gatccggcat gcgaaattcc cccgatgcct ccagaccggg
     3901 gtccataccg gacggcagat tctccagccg gtacgacgcg gcggcgacgt catcaaaact
     3961 caccgagcgg ccgatcgttc cacgaacggc gaaatgaccc tcggtcagtt cgatatcacc
     4021 ttcggcagcc tccaggaggt gcgcggcgat cttgattgcc tttgcgcgaa cctcatcgag
     4081 cgctagtatc agcgcggagc ctccgagcgt ggtcgagcgg cttccaaatg ttcccacgcc
     4141 ctccggcatt cgagcggtgt cgccgaattc gaccgtcacc gcatcgagat cgagctgtag
     4201 attatcggca gcgatctgcg caaatattgt ttcgtgtccc tggccgtgcg acgatgagcc
     4261 tgggcgcacc cggaccaagc catcgggatc aacccgcacg tcggtgcgct cccatacggc
     4321 cggggccgct cgctcgacat agacggcaac gccgatcccc aagaggcgcc cggccgcgcg
     4381 ggcctcgcgt tggcgtactt tccacccatc gtagtccagg agctccaacg cacggtcgag
     4441 cgcacgctca tagtttcctg aatcgtagac cagacccgtc ggcgtcttgt aggggaatcg
     4501 attcgaagga ataagattcc cgcggcggac ctcaacagga tcgagtgcca gctctctcgc
     4561 gacgagatca agcatgcgct cgacgatgta tgcggcctcc ggacggcccg cgccccgata
     4621 gggtccgatc gggactttgt tggttgtcag accgacggcg tcaacctgcg cgcttgggat
     4681 ctcatacgct ccggtgagca tcatcgcggt aatgatcggg cggctggcag tcggggcata
     4741 aagataggct cccaggtcgc caacgatgcg gcctttgacc gcgagaattt ttccatcgcg
     4801 ggtgacggcc atttgcacgt cggcctcgac acctcgaccc tggtaggccg ccaggaaatt
     4861 ctcccgccga tcctctaccc acttcacggg cctttcggtg ctcacggcaa gaaatgcggc
     4921 cagggcctgt tcgggagcca gacttccctt ggtgccgaaa gccccgccga catcctgcac
     4981 gatcacacgg atccggtcct gagagcgatt tagaacgtgg ctgaggtgcg tcctcggcct
     5041 gtagggatct tgcgcagaac accacacggt gagtaagtcg gtctcccgat cgtaggaggc
     5101 cacaacacct cttgtctcaa tcggtacggc taccaagcgg ggaatgcgaa acttcccgtt
     5161 cacgacccga tcggctctcg agaatgcgcc gtccacgtcg ccacctgtgt gttcccatct
     5221 gacgacgacg ttctcgccga cggcctcgtg cactaacgtc gttccattta gcgcatctga
     5281 tacgtcgacg acctcgggca aagggtcgta atcgacctcg attgtcgccg ccgcatcaag
     5341 cgctgccgca cgggtctggg cgagcacggc tgcaacgggt tcgcctgtgt accgtacctt
     5401 gtccgttgcg agcagcggat gtggaatatc ggcgatcgtc atcccctcct ctgctcgcag
     5461 tggcatcgaa cggactttgc cgatcagatc ctgcgcggtg acaatcgcga cgactcctgg
     5521 gatagccatg gcttcgtcgg tgcggattcg gcggagcttt gcgtgagcct gagaactcct
     5581 gacgacaacc atgtggagca tcccgggaag cgtgagatca tcgatgtagc gtccccggcc
     5641 ccggaggaat cgctcgtcct cccggcgttt gatggggcgg ccaatccagt gaatgcgtgg
     5701 catcaggttt tatatacgcg caagtaagga gcgagcctgc gatttgtcga accgaatttg
     5761 acacaccgaa tgcagaagat ttcatctgtt ggcatcttgg ggcttgggcg gatgggcttg
     5821 cccatgggtc ggcatctgct tcgggccggc tttgcggtcc acggattcga tcctcacgag
     5881 gctcaactgg cagcactctc ggatgcgggg ggaaaagcgc tgcgttccgc ttctgcagtt
     5941 gccgggaaga gcgaggctat tctgatcatg gtggcagacg acgcccaggt caaagaggtc
     6001 acgatgggct ccggcggcgt gttcgaagct gcgaagcctg gcactgtttg catcatctcc
     6061 agcactgtga aaccttcgac ctgccgtgaa atcgctacgg ctgctgagaa acgaggcatc
     6121 aaggtgctcg atgcgccggt cgcgagaggc cagcgcgccg ctgaggaggg gacactcacc
     6181 gtgttcgccg gcggcgagcg gtcaacgttc gagcaatgcc gcccgatctt cgaggcattc
     6241 ggcaaagaag tcgtacacat cgatgagcgc gtgggctcgg ggcaggtcgc aaaactggtg
     6301 aataacctga tcctatggg
//
LOCUS       DBNGSB010000198         5249 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3823929, whole genome shotgun sequence.
ACCESSION   DBNGSB010000198 DBNGSB010000000
VERSION     DBNGSB010000198.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5249)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3823929"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..1099
                     /locus_tag="AC159B_05805"
     CDS             68..1099
                     /locus_tag="AC159B_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3377293.1"
                     /translation="METFQQSSATMRKVLVEIKRIIVGQDLMLERILVALLARGHVLI
                     EGVPGLAKTLAIKTTARVIDCDFKRIQFTPDLVPADLVGTRIYNQHAGTFDTELGPVF
                     ANLVLADEINRAPAKVQSALLESMQERQVTIGKQTFFLPDPFLVLATQNPIESEGTYP
                     LPEAQVDRFMFKIVITYPSQHEEITVVERMTIEHPDVQVVVKSADLLELQRVADAIYV
                     HPKIMEYAVQLANATRRPKDFGLNDLTPYVSYGSSPRASLNLILGGKALALIRGREFV
                     LPEDVRDIAPEVMRHRLVLSYEALAQDINGDYIVKRVIDSIPAPRVHIGDPYDRTASP
                     ASTAGAKTG"
     gene            1136..2029
                     /locus_tag="AC159B_05810"
     CDS             1136..2029
                     /locus_tag="AC159B_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR3377294.1"
                     /translation="METPERVLRRLEFKIVRRLDGFLFGDYRGVFYGPSLDLAEVREY
                     QPGDEVRRIDWNVTARMSRIFVRQYLEERELTAWLAVDLSPSMRFGTRRQLKRDTATE
                     FSAVASYIVTRHGDKVGLIGFPGRHVFIPPRGGRMQALRVIHTLDSLSEPERSVSDLG
                     ESLRMVAKTIRRRSLLFVVSDFQAKDGWEQPLRELVRRHEVIAVWVTDPAERELPDVG
                     GLFVRDPETGEQVWVDTSDRRLRESYRSLVAAQEQKIRRAFRLAMVDVLELSTAEPLV
                     GPLLRFITRRKRRSRWNLAGR"
     gene            2008..3045
                     /locus_tag="AC159B_05815"
     CDS             2008..3045
                     /locus_tag="AC159B_05815"
                     /inference="COORDINATES: protein motif:HMM:NF024909.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3377295.1"
                     /translation="MEFGWPVMLWGLLAVPVLLWVYVRVQRFRVRREGALADAHLLGH
                     LYTRPPAIRRHLPVTFYLIATMCLLLALARPIAAIPLPVNRAALILAIDVSKSMIGED
                     VKPSRMQATKGAANAVLDAIPGSAKVGLIVFSDYAQVLVPPTTEREVIREAINGLKFL
                     QATGIGSAILEALRALPGRKELLGERLNPPPVPGQQMPVPLPAPPGPPSTVRPEDLPP
                     AAIIIFSDGVSNLGTNPEQAAALAKEGKVKVYGVGVGATGGSVMLIDGQLSLVPFDPT
                     LLQRISQLTDGKYYSITETDELKRLYRQLGRSMGWERRRTEITSVLAGGAGFLMLVGG
                     LFSLIWFRRVP"
     gene            3065..4042
                     /locus_tag="AC159B_05820"
     CDS             3065..4042
                     /locus_tag="AC159B_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012321.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3377296.1"
                     /translation="MTFQWPAMLLTLLLLLPLAALYRWVLRRPPRQATRYTRVPLLAE
                     AAARAGRWRRHVPAGLFAISLAAVMVALARPVAPMPVPSTKNAVVLSIDVSRSMLADD
                     LPPNRMEAAKQAAREFVENLPQGIKVGLVTFSSYATLIVPPTADRVRVIDAINQLHTE
                     FATAIGDGLVEAVFALPGRERPATLTTPAPPPKQPVPPGTVVLLSDGQSNRGVLPADA
                     ARIAREQEAKVYTVGVGTPEGTFLNLGGRSIWVRLDEDALREMAEIGGGSYYHARSVA
                     ELRSAYKRLSRVVGWESRPTEVTGLASGVAAVFLLAALGASFLSVNRLA"
     gene            complement(4039..>5249)
                     /locus_tag="AC159B_05825"
     CDS             complement(4039..>5249)
                     /locus_tag="AC159B_05825"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR3377297.1"
                     /translation="PSPADAAYLLFYPILLIGLLSFPSAPQERVVRATFWLDAATVLL
                     SGWMIVWYFVLGPTALEQHADLLTIVLSSAYPIGDLVIIFGIAWVLLRRPEEGSRQAL
                     GLLAAGVASFLIADLAFGHLSLSDAYKSGDWPDAFWMVALFLMVCSAQWQVWSTGRQR
                     HEEPRAEAEVGSASTLPYLAVALGYAMLFFAGRHAAAYPIGGLLSGAIAITAVVLARQ
                     LTVMNENIRLLGKLHELATTDSLTGSMTRRHFFDLAHREFSRAKRYSRKLSAIMIDVD
                     FFKAINDRYGHASGDEALQAIVTRCRANLREIDFLGRYGGDELVILMPEVDLRDATRA
                     AERVLAAVAETPLVTNGSRIDLSVSAGVASVEDSTDLATMLRRADVALYEAKQAGRNC
                     IRTAATDSRV"
BASE COUNT          989 a         1614 c         1677 g          969 t
ORIGIN      
        1 cgtccctgtg gcataatagg aaaaattcgg ctctagcaga ggagaccaga acgtgaccac
       61 gcttcccatg gaaaccttcc aacagtcctc ggcgacaatg cgcaaggtgc tcgtggaaat
      121 caagcgcatc atcgtcggtc aggacctcat gctcgagcgc atcctggtag cgctgctggc
      181 ccggggccac gttctcatcg aaggagtccc cggcttggcg aaaaccctgg ccatcaagac
      241 cactgctcgc gtcatcgact gtgacttcaa gcgaattcag ttcacacccg atctggtgcc
      301 cgccgacctg gtcggtacgc ggatctacaa ccagcacgcc ggtacctttg atactgaact
      361 tggacctgtc ttcgcaaacc tcgtgctggc cgacgagatc aaccgcgccc cggcgaaagt
      421 gcagtcggcg ctgctggagt cgatgcagga gcggcaggtt acgatcggca agcagacgtt
      481 cttcctgccg gaccccttcc tcgtcctggc cacgcagaac ccgatagaaa gcgaagggac
      541 gtacccgctg cctgaagcgc aggtcgaccg gttcatgttc aaaatcgtga tcacataccc
      601 gagccagcat gaagagatta cggtcgtgga gcggatgacg attgagcatc cggatgttca
      661 ggtcgttgta aagtcagccg atcttctcga gctgcaacgg gtggcggatg cgatctatgt
      721 gcacccaaag atcatggagt atgcggtcca gctggcgaat gcgacgcgcc ggccaaagga
      781 cttcgggctc aacgacctga cgccgtatgt ttcctacggc agtagtcccc gcgcgtcgct
      841 caatctgatc ctgggcggca aagcgctggc cctgatccgg gggcgggagt ttgtgctgcc
      901 cgaggacgta cgcgacatcg cgccggaagt gatgcgccat cgcctggtcc tgtcctatga
      961 ggcgctcgcg caggatatca atggcgatta cattgtgaag cgcgtcatcg atagcatccc
     1021 ggcgcctcgc gtgcacatcg gcgatccgta cgatcgcacc gcctcgcccg cttctacggc
     1081 cggcgccaag actgggtagc cggggagcca ggtagacaac gactcagagg ctttcgtgga
     1141 aactcccgag agagttcttc ggcgcttaga gttcaagatc gtccgcaggc tggacggctt
     1201 cctgtttggg gactaccggg gagtcttcta cggtcccagc ctcgatctgg cagaagtgcg
     1261 cgagtaccaa ccgggggacg aagttcgccg gatcgactgg aacgtcactg cacgcatgtc
     1321 acgaattttc gtccggcaat accttgaaga acgcgaactc acggcgtggc tcgccgtcga
     1381 cctctcgccg tcgatgcggt ttggaacccg gcgccagttg aagcgggaca ccgcgacgga
     1441 gttctctgcc gttgcgtctt acattgtcac ccggcacggc gacaaggtcg gtctcatcgg
     1501 gttccccggg cggcatgtgt tcatcccacc gcgtggggga aggatgcagg cgctccgcgt
     1561 gatccacaca ctcgattcac tttcggagcc tgaacggagc gtctccgatc tcggcgagag
     1621 cctgcggatg gtcgccaaga cgatacggcg gcgaagcctg ctcttcgtgg tctcggattt
     1681 tcaggccaag gacgggtggg aacagcctct gcgcgagctc gtgcgccggc acgaggtcat
     1741 cgcggtatgg gtcacggatc cggctgagcg cgagttgccc gacgtcggtg gcttgttcgt
     1801 ccgcgatcct gaaacgggtg agcaagtctg ggtggacaca tcggatagac ggctgcggga
     1861 gtcatatcga agccttgtcg ccgcgcaaga acagaagatc cgccgggcgt ttcgtcttgc
     1921 catggtagac gtcctcgagc tttctacagc cgagccgctc gtgggacctt tgctccggtt
     1981 catcacacgc agaaaaagga gaagtcggtg gaatttggct ggccggtgat gctgtgggga
     2041 ctgctggcag tcccggtgct tctgtgggtg tacgttcgcg tgcagcggtt ccgagtcagg
     2101 cgcgaggggg cgctcgccga cgcgcacctc ctgggccacc tatacacgcg gccgccggcg
     2161 atccgccggc atctgccggt gacgttttat ctcatcgcca ccatgtgctt gctgctcgcg
     2221 ctggcgcggc cgatcgctgc gattccactg cctgtgaacc gcgcggcgct gatccttgcg
     2281 attgacgtga gcaagagcat gatcggcgag gatgtgaagc cgagccggat gcaggcgacg
     2341 aagggtgccg ccaacgccgt gctcgacgcc atccccggct cggccaaggt cggtctgatc
     2401 gtcttcagcg attacgcgca ggtcctcgtg ccgcccacga cggagcgcga ggtaatccgc
     2461 gaagccatca acggactcaa gttcctgcag gccacgggga taggctcggc cattctcgaa
     2521 gcgctccgcg ccctgcccgg acggaaggaa ctcttagggg agagactcaa cccaccaccg
     2581 gtgcccgggc agcagatgcc cgtgccgttg ccggcgccgc ccggaccgcc gtcgaccgtc
     2641 cggccggagg accttccgcc tgcggccatc atcatcttct ctgacggcgt gagcaacctg
     2701 ggaaccaacc cggaacaggc cgccgcgctc gccaaagagg gcaaagtcaa ggtatatggg
     2761 gtcggcgttg gggcgacggg tggaagcgtg atgctcatcg atgggcaact gtcgctcgtg
     2821 ccttttgacc cgaccctgct gcagcgaatt tcacagttga cggatggaaa gtactactct
     2881 attactgaga cagacgagct gaaacggctt taccggcagc tcgggcgttc gatgggatgg
     2941 gagcggcggc ggacggagat tacgtccgtg ctggctggtg gtgcaggatt cctgatgctt
     3001 gtcggtggat tgttctctct aatttggttc agacgagtgc cgtgaattgg aggatcggta
     3061 gccagtgaca tttcagtggc cggcgatgct gctgacgctc ctgctgctgc tccccctggc
     3121 ggcgttgtac cggtgggtcc tgcggcggcc gccacggcag gccacccggt atacccgcgt
     3181 cccactcctc gctgaggctg cggcacgggc cggtcgctgg cgcaggcacg ttccggcagg
     3241 gctgttcgcg atatcgctgg cagcggtaat ggtggcgctt gcgcgtccgg tggcgccgat
     3301 gccggtcccg tcgacgaaga acgcggttgt gctctccatc gacgtgagcc ggagcatgct
     3361 ggccgacgat ttgccgccaa accggatgga agccgcgaag caagccgcgc gtgagttcgt
     3421 ggagaatctg cctcagggga tcaaggtcgg gctcgtgacc ttcagcagtt acgcaacgct
     3481 gatcgttccg ccgaccgcgg accgtgttcg ggtcatcgac gccattaacc agctccacac
     3541 cgagtttgcg actgccatcg gtgacggcct cgtagaggcc gtctttgcac tcccgggccg
     3601 cgagcgacca gcgacgctga cgacccccgc tccgcctccc aagcaaccgg ttcctccagg
     3661 caccgtggtc ctactctccg atgggcagag caatcgcgga gtactcccgg ccgacgctgc
     3721 ccgcatcgcc agggaacagg aagcgaaagt ctacacggtg ggcgtgggga caccggaagg
     3781 aacgtttctt aatcttggcg gccgcagcat ctgggtccgc ctcgacgaag acgcgctgcg
     3841 cgagatggcg gagatcggcg gcggctcgta ttaccacgcg cgatcggtgg cggaactccg
     3901 cagtgcatac aagcggctca gccgcgtggt tggatgggag tcgaggccga cggaagtcac
     3961 gggtctggcc tccggcgtcg cagcggtctt tctcctcgca gcgctcggcg cctcgttcct
     4021 cagcgttaac cgcctcgcct aaaccctcga atcagtagcc gcggtacgga tgcagttgcg
     4081 tcccgcttgt tttgcttcgt agagtgcgac atctgcacgc cggagcatcg tggctagatc
     4141 tgtgctgtct tccaccgatg ccactcccgc gctcaccgac aggtcaatcc gggacccatt
     4201 tgtgacaaga ggcgtctcgg ccacggcggc cagcacgcgt tcggccgcgc gggtagcatc
     4261 ccggagatca acctccggca tgagaatgac gagttcatct cctccatagc ggcccaggaa
     4321 gtcgatctcc cgcaggttgg cccggcatcg tgtgacgatc gcctgcaggg cctcatcacc
     4381 cgacgcgtgt ccataacggt cgttgatagc tttgaaaaag tctacgtcga tcatgatcgc
     4441 cgaaagcttc cggctgtacc gcttcgcgcg cgagaactcg cgatgggcca gatcaaagaa
     4501 gtgccgccgg gtcatcgatc cggtgagact gtccgtggtc gccagttcgt gcaacttgcc
     4561 gagcagccgg atgttttcat tcatgacggt gagctggcgg gcgagtacga cggcggtgat
     4621 tgcgatcgcg cccgagagca gccctccaat cggatacgcg gcggcatggc ggccggcgaa
     4681 gaatagcatg gcgtacccaa gcgccacggc cagatagggg agtgtgctcg ctgacccgac
     4741 ctcagcctcc gctcggggtt cctcgtgccg ctggcgtcca gtactccaga cctgccactg
     4801 agcactgcac accatgagaa agagagccac catccagaag gcgtcgggcc agtccccgct
     4861 tttatatgcg tcgctgaggg acagatgccc aaaagccagg tcggcgatca agaatgaggc
     4921 gacacctgct gcgaggagcc ccaacgcttg gcggctgccc tcttcaggcc gccgcaagag
     4981 tacccaggcg atcccaaaga tgatcacgag atcgccgatg ggatacgccg acgagagcac
     5041 gatcgtgagg agatcagcat gttgttcgag agcggtcggt cccagcacaa agtaccacac
     5101 gatcatccac ccgctcagca ggacggtcgc cgcgtcaagc cagaacgtcg ctctgacgac
     5161 tcgttcctgt ggcgcggacg gaaatgaaag aagaccgatg agtaggatcg gatagaacag
     5221 gaggtaagca gcatcagcgg gcgacggaa
//
LOCUS       DBNGSB010000199         3203 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_144150, whole genome shotgun sequence.
ACCESSION   DBNGSB010000199 DBNGSB010000000
VERSION     DBNGSB010000199.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_144150"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(380..2593)
                     /locus_tag="AC159B_05830"
     CDS             complement(380..2593)
                     /locus_tag="AC159B_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE family DNA translocase"
                     /protein_id="HIR3377298.1"
                     /translation="MGRKKQVTSRKPAAGVMLLIVGLLLTLSFLPRATGVAITLGRWQ
                     RLILGEAALLLPVFVGWAGLYLLVAGGRAQFTHRFIAVISLSLAGITAYHARIAPGSE
                     FVAAGKGLGSGVLGGALVWVLRMLLGEVGMWLVISLAILAGVVLGSGASLSEWRDVFA
                     RVTFFLAYRVRAGLASLLGLIWASGVAIVSGFTRLVHLVAARQVRQAAVPMTEPPIIA
                     APLAEQPSHSTSTAPVREPAVTTVAREIPRADYTQESLELEAPSGGYQLPAISLLADL
                     PGIRGKGKAEPSEVARALEQTMASFGVEAKVLRWEQGPVVTRFEVQPAPGVRVQKISS
                     LTNDIALALAAPSVRIEAPIPGKSAVGIELPNQKTSLVHIKEILASEEYLRATTPLTV
                     AIGKDIAGHPILADLAEMPHVLIAGATGSGKSVMLNAIIAGLLFRCTPLDVRLLMIDP
                     KRVEFTNYNNVPHLLVPVVTNPRTAAGALKQMLAEMEERFERFAGAGVRNIQAYNQLT
                     DVERLPFIVIVIDELADLMMVAPADFEDVIVRLCQMTRATGIHLVVATQRPSVDVITG
                     LIKANIPSRIAFAVSSQVDSRTILDMPGAEKLLGKGDMLFMPVAAPRPTRAQGAFIAD
                     SEVQALVSWWKSQGRPAYDQKLIHAEKSSAEGQGDGDELLVEAARLIVRSGYGSVSLL
                     QRKMKIGYVRAARIVDQLEEKGIVGPTQGSNPREVLVGIDELDRHLGAKARSSAE"
     gene            complement(2597..>3203)
                     /locus_tag="AC159B_05835"
     CDS             complement(2597..>3203)
                     /locus_tag="AC159B_05835"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIR3377299.1"
                     /translation="DVRRLLVGWGGGGEAAYRRLAWLIILATAITASIGLLFAPQFER
                     MYGSLRGVAFQLILTGLVLLLARERGGRQITDLSISDAALLGLAQAVSIVPGISRSAA
                     TIAGGMWLGLRRDEAARLSFLIAIPSVAGAGLFGLKDLRVGASLGYGPFTLLVGFTVA
                     LISGVVAIKWLLSIVRRGRLGMFAAYCIVAGLIVLAATFRG"
BASE COUNT          646 a         1049 c          946 g          562 t
ORIGIN      
        1 gtcattgaac gagagatcgc cgcgtccacc gacgtcgtag acgaggccga cgcgatattt
       61 cttcgcctgg gcctgcactg ctgatccagg gagtatccat aaggtggcag ccatcatgac
      121 gactatcccc agcgccacca gacgcaagct gcgcttgctc atacctctgc acctcctgta
      181 agatgcgacc ttcacacttt tttgccgcag ttcccggcgc tatttatgca cagcacagcc
      241 gggtagcacg ccttgccccg tggtcggcca ttgtttcggc gccctcttgc tcatcccttt
      301 cagaagtgcc gccatccggg tacctggtac cggaagccct cccaaaaagt acctggtacc
      361 gattttcggc caaatttaac tattcggcgg acgaccgggc tttggctccg aggtggcggt
      421 cgagttcgtc gatgccgacg aggacttcgc gcgggttgct gccttgcgtg gggccgacga
      481 tgcctttctc ttccagttga tcgacgatcc gggcggcacg gacgtaaccg atcttcatct
      541 ttcgttggag cagtgacact gacccgtagc cggaacgcac aatgaggcgg gcggcttcga
      601 ccagcaactc gtccccatca ccctggcctt cggccgagct cttctccgcg tggatgagct
      661 tctgatcata ggcaggccgg ccctgggatt tccaccagct gaccagcgcc tgcacttcgc
      721 tgtcggcaat gaatgcgccc tgggcgcggg tgggacgggg ggcggcgacc ggcatgaaca
      781 gcatatcgcc tttgcccaac agcttttcag cgccgggcat gtccagaatc gtacgactat
      841 cgacctggct gctgacggcg aatgcgatgc gagacgggat gtttgctttg atcagcccgg
      901 tgatgacgtc tacggacgga cgctgtgtcg cgacgacgag gtgaatcccc gtggcccgcg
      961 tcatctgaca caaccggacg atgacgtctt cgaagtcagc cggcgccacc atcatgaggt
     1021 cggcgagctc atcgatgaca atcacgatga acggtaaccg ctcgacatcg gtgagttggt
     1081 tataggcctg gatgttgcgc acgccggctc ccgcgaaccg ttcgaaccgt tcctccatct
     1141 ctgccagcat ttgcttgagc gcaccggcgg cggtccgtgg gttggtgacc accggcacga
     1201 gcaaatgcgg aacgttattg tagtttgtga actcgacgcg cttgggatcg atcatcaaga
     1261 ggcgcacgtc gaggggcgtg catcgaaaca gcagtcccgc gataatcgcg ttgagcatga
     1321 cggactttcc ggaaccggtg gcgcccgcga tgagcacgtg gggcatttcc gccagatcag
     1381 ccagaatggg atggccggca atgtctttgc caatagccac ggttaacggc gtggtcgcac
     1441 gcaggtactc ctcgctggct aaaatctctt tgatgtgcac caggctcgtc ttctggttgg
     1501 gcaactcgat cccgacggcc gacttcccag ggatcggggc ttcaatgcgc acgctcggcg
     1561 cggcgagggc aagggcaata tcgttcgtga ggctgctgat cttctgtacg cgcacgcccg
     1621 gtgcgggctg gacttcgaaa cgcgtgacca ccggcccctg ttcccagcgg agcactttgg
     1681 cctccacccc gaaactggcc atggtttgct cgagggccct ggccacttcg gaaggctcgg
     1741 ccttgccttt ccctcgtatc ccgggaagat cggcgagcag cgatatcgcc ggcagttgat
     1801 atccaccgga aggtgcttcg agttccaatg actcctgtgt gtaatccgct cttggaatct
     1861 ctcgtgccac ggtagtcacc gcgggctcac ggaccggcgc agtcgaggta gagtgactgg
     1921 gctgttcagc cagtggcgca gcgatgatcg gcggttcggt catcggaacc gcagcctggc
     1981 gaacttgacg agcggccacc agatggacca gtcgcgtgaa tccagacacg atggcaacgc
     2041 cgctggccca gatcaggccc agcaggctcg cgagccccgc cctcacacgg tatgcgagaa
     2101 aaaacgtcac cctcgcaaag acgtcacgcc attctgacaa cgaggcccca gagccgagta
     2161 cgacgcccgc gaggattgcc agcgagatga ccagccacat ccccacctcg cccaaaagca
     2221 ttcgcagtac ccacacgaga gcgccgccga ggacgcctga tcccagtccc ttgccggcgg
     2281 ccacaaactc cgaccccggc gcgatgcgtg cgtggtaggc ggtgatcccg gcgagcgaca
     2341 aagaaattac ggcaatgaac cggtgtgtga actgtgcccg gccgcctgcg accagcagat
     2401 acaggcctgc ccagcccacg aataccggca gcagcagcgc cgcttcgccg aggatgaggc
     2461 gctgccacct gcccagcgtg atcgccaccc ctgtcgctct ggggagaaac gacagcgtca
     2521 ggagcagacc gactatgagc agcatcaccc cggccgccgg cttgcgcgat gtgacctgtt
     2581 tcttacgccc cacgagtcat cctcggaacg tcgcggccaa cacgatcagc cccgcgacga
     2641 tgcaataggc cgcgaacatc cccagccgac cgcggcggac gattgacaac agccacttga
     2701 tcgccaccac tcccgaaatc aacgcgacag tgaaaccaac cagcagcgta aacggaccgt
     2761 agccgagcga tgctcccacc cgcaggtcct tcagcccaaa caggcccgcg ccggcgacgg
     2821 aagggatggc gatgagaaac gaaagcctgg cagcttcatc acggcggagc ccgagccaca
     2881 ttccgccggc aatcgtcgcg gccgaccgcg agatccctgg aactatggac accgcctgcg
     2941 ccaatcccaa caatgcagcg tcagagatgc tcaagtctgt gatctgcctg cccccgcgct
     3001 cgcgagcgag cagcaagacc aatccggtga ggatcaactg gaatgccaca ccacgaagcg
     3061 aaccgtacat ccgctcaaac tgcggggcga acagcaatcc gatcgatgcc gtgatggctg
     3121 tggcaaggat gatgagccac gcgaggcgac ggtaggctgc ctcccccccc cccccccacc
     3181 caaccaagag gcggcgcacg tca
//
LOCUS       DBNGSB010000200        11940 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_1372451, whole genome shotgun sequence.
ACCESSION   DBNGSB010000200 DBNGSB010000000
VERSION     DBNGSB010000200.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 11940)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11940)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11940
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_1372451"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="AC159B_05840"
     CDS             <1..780
                     /locus_tag="AC159B_05840"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3377300.1"
                     /translation="GLDRPVYIQYGRWLGRVLSGNLGTSVRTGRPIGALVLEALPFTL
                     ELAIYAVVLAVLIAVPVGTLAGTTSSRLADGTMQTLTLLGLSLPAFWVGAMFILLFSV
                     HLRWFPVLTFPSFLEAPLANLRGFFLPALTLAVPNAAAIARMVRSSLVSVRREEYVKV
                     ARAKGLSERLVVRRHMLKNALIPVVTLIGIVAGYLLGGSIVVEQVFAIPGVGRMGLQA
                     IVQRDYPVLQAVVLIVTALFVLMNLLVDLIYVFLDPRIRYA"
     gene            746..1654
                     /locus_tag="AC159B_05845"
     CDS             746..1654
                     /locus_tag="AC159B_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015231367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3377301.1"
                     /translation="MSSSIQESATPDPTRAPEVIRPRSLTRVALRSPKAVVGMIVLLL
                     VTGLAILAPLLAPYPATEMHAIDRFAPPGRPYLLGTDFFGRDLFSRILYGYRTSLLVA
                     VLSVAFALVVGSAFGVVAGYTGGPVDSAVMRTMDILFAFPVLLLAITVVVILGAGMVT
                     TVLAIGIVYVPIFARTARGPTLVVREHAYVEAAKAIGMRSLRIMRRHILPNVSTPIFV
                     QATINLATAILFESALSFLGLGTQPPYPSLGLMVSEGRNYLELSPWPTVFPGLAIVLA
                     VLGFNLVGDALQEILDPRLRQTWSAA"
     gene            1670..2683
                     /locus_tag="AC159B_05850"
     CDS             1670..2683
                     /locus_tag="AC159B_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3377302.1"
                     /translation="MRHYLAQRIARAVLTIFLVVSFTFFLVRLMPGNPIDLYVQEQVT
                     TYGQTYEQALEQARGFFALDLDAPLSVQYAQYLGNLARGDLGQSILSPGTPVSSLIKK
                     FLPWTLFSVGLALMISFTLGVTLGMLMAYRRESWIDHVLTVIATVTTAVPNYLIGILL
                     LVVLGVQTNLINVAAIRGSLSPGVRPGLTLTFLLDALSHAALPITTYVMATAGSWMLS
                     MKSNTLGTLGEDYVGFARARGLPDRRIAVAYVGRNALLPLFTQLTVAIGFVVGGSALI
                     EYIFNYQGIGLVLYDSIVRRDYTVMQGVFLVITVSVVLAILRADLLYGRLDPRVDLAA
                     EAI"
     gene            2680..3513
                     /locus_tag="AC159B_05855"
     CDS             2680..3513
                     /locus_tag="AC159B_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3377303.1"
                     /translation="MNWDALKRNPSGALGLAGFLAIALLVVIGPVVIPLDATTRLDQI
                     YQPPSTAHPFGTDNQGRDVFSQIVHGGKDLLWVATLAAGISTVIALALGSMSAVAGGS
                     IDNALMTLTDVVLTVPHFPLLAVLAGVLQFRSQTLLAIILGVLSWPTLTRAVRAQVLS
                     LKERDFVEAAEALGLGRGHIIAREILPNMRSYVAISFVLAMTQAIYAQVGLVFLGLVP
                     LSGQNWGVMIQYGWTRGAIFHKSSVYSILAPIMAIAVLKLSLVSMARLFDEIFNPRLQ
                     G"
     gene            3510..4559
                     /locus_tag="AC159B_05860"
     CDS             3510..4559
                     /locus_tag="AC159B_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013015905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3377304.1"
                     /translation="MTDELISIRDLSIVYRGRGGSAQALREVSLDLYRDETLGLIGES
                     GSGKTTLALAVLRMLPTAAHIPHGKILYHQDGRTVDVFSLDAERLRRYRGEQVAMVFQ
                     SALSSLNPVLRIWDQFLDTARAHGEFDQVRVQARTRELLSHVQLEPGRVLGAFPHELS
                     GGMRQRVMLALALLLRPRVVVLDEPTTALDILTQRTIIDLLRSLRKEFGFSMMFISHD
                     LSLAAELADRVATMYAGRIVEIGTTDELFYEPRHPYTVGLLQAVPTVHGGHRPLQSIP
                     GSPPDLQRLPSGCKFHPRCKYARPTCAETEPDLLHVSDGHRSACFFWDEVRKDAQARN
                     RGSTQTRPLDLEASQ"
     gene            4556..5548
                     /locus_tag="AC159B_05865"
     CDS             4556..5548
                     /locus_tag="AC159B_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HIR3377305.1"
                     /translation="MIILEHVGRVFRSRGQILRAVDDVSLEIGEGQVMSLVGESGSGK
                     TTTGKMIAGLVTPSSGRVLFEGRDIRTLEGDARGQYRRAVQIVHQDPYACLNPVRTVG
                     DTLAAPLRLYGLADGRSRNAGIGELLERVELTPASAFIDKYPHQLSGGQRQRVAVARA
                     LSVRPKFLVADEAVSMIDVSLRISLLNLLLKLRQETGVAFLFITHDLALARHFGWDGR
                     IAVMYLGRVVEVGPTPGVIEDPQHPYTRALLAAVPEADPRITRTKPRLRLRSQDPPSL
                     LHLPPGCAFHPRCPWAESGLCDVIRPDLTHVRAQEVACHVAAREGALPELAENV"
     gene            5560..5736
                     /locus_tag="AC159B_05870"
     CDS             5560..5736
                     /locus_tag="AC159B_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3377306.1"
                     /translation="MHPQLARAALRIGVFIALTSGVLTFIEPRGSPEKAISLWTLLAA
                     LLFLAVIVARMRRV"
     gene            5796..7547
                     /locus_tag="AC159B_05875"
     CDS             5796..7547
                     /locus_tag="AC159B_05875"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3377307.1"
                     /translation="MRKAMTIILTALLLGFSILPHDAMGAPAGSVFQGAWPYQAPPAG
                     HYNSFIPGYLIPFVNPYNDLVEMPLAFYLWQKNEYIPVLAAQWKAEAGGRAFTVSLRP
                     GVRWSDGSAFTSADVASTFYIGYLFGFLAWRSISGIETPNDQTVVFRFTTPASIALRT
                     ILRVNIRPRSVYGALADRAKPYAEQRMALTDEPVRTLTDEVRRFRPRESVATGPFRID
                     ASSITEAQLTLVKNPTSFSANQVQFDRIVLFNGETPQITPLALSKQIDYATHAFPPAT
                     DRQFQELGVRVLRAPVYSGPALFFNHRISPLNRKEVRQAIAYAVNRDQSGTVALGASG
                     RGVKVLTGFSDNLADNWLTADVKSKLNSYRYDPPRAAQILTRAGFKKGTDGVWVSDKG
                     ERMEYELSVPPEFADWSAAAEEVAAQLTRFGIRTTVRGVPNPQHILNVNEGRFQIALR
                     TWGAGNPHPFFAFSQDLFIHNYVVNAVTRGMDFSLRQKTDAMGEVDLASMVDSSGAGT
                     NLARQKSAVSRLALAFNELLPIIPLFERYSNSPVAEGLRVTGWPRDDDPIYKNHLGTD
                     SFVTIMIFNGTLRPAGR"
     gene            7544..8683
                     /locus_tag="AC159B_05880"
     CDS             7544..8683
                     /locus_tag="AC159B_05880"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3377308.1"
                     /translation="MTVALRSVTPREAGLDSDRVRTIEDLIDEGIRTGVFPGAVALVA
                     RGDTVGWQSARGDAQITPERRPAALDTIYDLASLTKVIAALPVALHVWQEGQLDLDAR
                     ISSVITAFTGPHKERVTFRHLLAHTSGLPSWRALYLKAKSREGVRDEICRTPLQAEPG
                     AAVEYSDLGILLLGFALEHITGRRIDDLVGEVVIRKLGLRDTMYNPPSFLHARCAATE
                     AGHGYEREKVGETGRGFRWRGTVLQGLVHDGNAHYGMGGIAPHAGLFSTAWEVATVAR
                     QWLRPGELVGAAALREATADQRHGAKGYPRGLGWVLHHEGTFFEALGARSFGHTGFTG
                     TSLAIDPDEELIVVLLTNRVHLGGGNVKIQDFRPRFHAVVRGAVL"
     gene            8728..9639
                     /locus_tag="AC159B_05885"
     CDS             8728..9639
                     /locus_tag="AC159B_05885"
                     /inference="COORDINATES: protein motif:HMM:NF013986.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HIR3377309.1"
                     /translation="MAAREDGTPVGSASAGPGNPHNIGPGPAATEIARAIREAASGET
                     PDVVVAGVAGLEDPELKRELRYALEALRPAQEIHVYSDAAVALDGAFLGGPGVIVIAG
                     TGSVAWGRNEKGQEARAGGWGHIIDDAGSGFDLGRQALAASLRAHDGRGPQTSLTRRL
                     TEALGVEGPLDFPGAARRLDPSRMASLARVVIEAADEGDLVARAIVRNAAGHLAEIAT
                     AVWKKLNLSRGHPVAPVGGLFSHARLRKAFASSLARRCPEAEVRPPRLPPVAGAVWKA
                     AVLKRLAPDEAMVMQLDRWAQTAAETR"
     gene            9609..10538
                     /gene="murQ"
                     /locus_tag="AC159B_05890"
     CDS             9609..10538
                     /gene="murQ"
                     /locus_tag="AC159B_05890"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007455067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HIR3377310.1"
                     /translation="MGPDRRRDPMNLRDEFGALSTEQRNAKSMQLDTMSTAQILELIN
                     DEDGTVAGAVRRALPEIVRATELVGGSIRSGGRVFYVGAGTSGRIGSLDASEWRPTFG
                     TDPDLVQAIMAGGASATVDAASGFEDDAALGARDVTGRGVRRGDVVVGLAASGRTPYA
                     IGALQAARAAGAATVGICCNPDAPMTTVVDVAIVVVVGPEVLTGSTRMKAGTAQKLIL
                     NMISTAAMVRGGKVYSNLMVDMAAGNEKLLDRARRIVAEAAGVPVGEAAKVLEAAGNR
                     VKPAIVMAVRGCNFDEAQQRLERAGGFVRKALE"
     gene            10552..11697
                     /locus_tag="AC159B_05895"
     CDS             10552..11697
                     /locus_tag="AC159B_05895"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HIR3377311.1"
                     /translation="MALHSWPTTKSPLRVIGLISGTSADGIDAAAVDVSRRAEGVDIR
                     TLATLASDFKPEQRKRILDLCRPDAALEAVCAANVDVAEWFADAALAAIKKVGRPSAE
                     FDLIASHGQTIWHIPGHSTMQIGEGAVIAERTGLPVVSNFRARDMAAGGQGAPLVSYL
                     DYLLFRDPARSRAAQNLGGIGNVTWIPANSAAGDVISFDTGPANMLIDGVAHLTAKRQ
                     RDDGGQIASRGQVQDRFLSELLADAYYDQPPPKTTGREKFGDAYARQIVDRGRNLRIK
                     DEDLLATVTMLTARTIAEAYRRFLGKVDEVILSGGGVHNQTLVQMIGTELAPAQVRIS
                     DAYGLDADFKEAAAFAVFGALTAWGQPSNLPAATGARRPVVLGDITP"
     gene            11707..>11940
                     /locus_tag="AC159B_05900"
     CDS             11707..>11940
                     /locus_tag="AC159B_05900"
                     /inference="COORDINATES: protein motif:HMM:NF015549.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HIR3377312.1"
                     /translation="MRSSISPVLISAIPLALAIGVFGTIFGAASTPLIGLELTLAASL
                     IVFSGALQFAMVGLLLAGAPVPSLLVTAALLNLR"
BASE COUNT         2026 a         3683 c         3848 g         2383 t
ORIGIN      
        1 gggctcgacc ggcccgtgta cattcagtac gggaggtggt tgggccgcgt gctgtccggg
       61 aatctcggca cgtccgtgcg caccggccgt ccgatcgggg cgctggtgct ggaagcgctg
      121 ccgttcacgc tcgagctcgc aatttacgcc gttgtgctgg ccgtgcttat tgcggtcccc
      181 gtcggtacgc tggcaggcac gacgtcgtcg cggcttgcgg atgggacgat gcagacgctg
      241 acgctccttg ggctctccct gccggcgttc tgggtcggcg cgatgttcat cctcttattc
      301 agcgtgcatc tcagatggtt tccggtgctg acgttcccgt cgttccttga agccccgctt
      361 gcgaacctcc gcggattctt tttgcccgcg ttgacgctcg ccgtcccgaa tgccgcggca
      421 atcgcccgga tggtgcgctc gtcgctggta tcggtgcgcc gagaggaata cgtcaaggtg
      481 gcgcgcgcga aagggctcag cgagcgcctg gtcgtgcgcc ggcacatgct caagaacgcg
      541 ctgattccgg tggtcaccct catcggtatc gtcgccggct acctcctcgg cggatccatt
      601 gtggtcgagc aggtttttgc gatcccgggc gtaggccgga tgggactgca ggccatcgtc
      661 cagcgagact acccggtgct gcaggccgtg gtgttgatcg tgaccgcact cttcgtgctc
      721 atgaacctgc ttgttgatct tatttatgtc ttcctcgatc caagaatccg ctacgcctga
      781 cccaacgcga gcacccgagg tcatccggcc gcgcagcctg actcgtgtcg cccttcgctc
      841 tccgaaggct gtcgtgggta tgatcgttct cctcctcgta acagggctgg caatcctggc
      901 tcccctcctc gctccatatc cggccaccga gatgcacgcc atcgaccggt tcgccccgcc
      961 cggcagaccc tatctgttgg gaacggactt cttcgggcgc gacctcttca gtcggatttt
     1021 gtacgggtac cgcacgtcac tgctggttgc ggtactgtcc gttgcattcg cgctcgtcgt
     1081 tgggagcgcg tttggtgtcg tcgcgggcta tacaggcggc ccggtggaca gcgcggtgat
     1141 gcggacaatg gacattcttt tcgcattccc ggtgctgctg ctggccatca cggtcgtggt
     1201 gatactggga gccggcatgg tcaccacggt gcttgcgatt ggtatcgtct acgtgccgat
     1261 ctttgcccgg actgcgcgcg gacccacgct ggtcgtgcgc gagcacgcgt atgtggaagc
     1321 ggcgaaagcc atcgggatga ggtcgctccg gatcatgcgc cggcacattc ttcccaacgt
     1381 cagcacgccg attttcgtcc aggcaacgat caacctcgcc actgcgatcc tctttgaatc
     1441 cgccctgagt tttctcgggc tgggaacgca gccgccctat ccgtcgctgg ggttgatggt
     1501 gagcgaagga cgtaactact tggagctcag tccctggccc acggttttcc ctgggctcgc
     1561 catcgtgctc gccgtgctcg gcttcaatct cgtcggggat gccttacagg aaatcctcga
     1621 tcccaggctt cgacagacgt ggtcggcggc gtaatatacg aagtagacta tgcgccatta
     1681 tctcgcgcag cgcatcgcac gcgccgtcct caccatcttc cttgtggtca gctttacctt
     1741 cttcctcgtc cggctgatgc cgggcaatcc catcgatctc tacgttcagg aacaggtgac
     1801 cacatatggc cagacctatg agcaggccct cgagcaggcg cgagggttct tcgcgctgga
     1861 cctcgatgcg ccgctcagcg tgcagtacgc gcagtatctg gggaatctag cgcgcggcga
     1921 tctcggccaa tccatcctgt ctcccggcac gccggtctcc tcattgatca agaagtttct
     1981 cccctggacg ctcttcagcg tcggactggc gctgatgatc agttttacat tgggggtaac
     2041 tctggggatg ctgatggcct accggcggga gagctggatc gatcacgtcc tcaccgtcat
     2101 cgcgacggtg acgacggcgg tgccaaacta cctcatcggc attctattgc tggtcgtgct
     2161 cggcgtgcag acgaacctga tcaacgtcgc cgcgattcga ggctcgctct cgcccggtgt
     2221 gcgtcccggc ctgacgctca catttttgct cgatgcactc tcccatgcag ccctccccat
     2281 cacgacgtac gtgatggcga cagcgggctc gtggatgctt tcgatgaaga gcaacaccct
     2341 ggggacgtta ggtgaggatt acgtggggtt tgcgcgggcc cgagggttgc cggaccgtcg
     2401 gattgcggtc gcgtatgtgg gccggaatgc tctgctcccc ttgttcaccc agctgacggt
     2461 cgctattgga ttcgtggttg gggggtcggc attgatcgag tacatcttca actaccaggg
     2521 gatcggtctc gtgctctacg acagcatcgt gcgccgggac tataccgtga tgcagggtgt
     2581 gttcctcgtg atcacggtgt cggtcgtgct cgccatcttg cgggcggatc tcctctacgg
     2641 gcggctggat ccccgagtgg atctcgccgc cgaagccata tgaactggga cgcactgaag
     2701 cgtaatccga gtggcgcgct tggcctggcc ggtttcctcg ctattgccct gttggtggtc
     2761 atcggtccag tggtgattcc cctggatgcg accacgaggc tcgatcagat ctaccagccg
     2821 ccgtcgactg cgcatccgtt cggcacggac aatcaaggac gagacgtatt cagccaaatc
     2881 gtgcatggag gcaaagacct gttgtgggtg gcgacgctgg ccgccggcat ttccaccgtg
     2941 atcgcgcttg cgctgggatc catgtccgcg gttgcgggtg ggtctatcga caatgcgctg
     3001 atgaccctca ccgacgtcgt gctcacggtg ccgcacttcc ctctgctggc ggtgcttgca
     3061 ggagtcctgc aatttcgctc ccagaccttg ctggcgatca tcctcggcgt gttgagctgg
     3121 cccaccctga cccgtgccgt ccgcgcccaa gtcctctccc tcaaggagcg cgattttgtc
     3181 gaggcggcgg aagcgctcgg cctcggacgc ggtcacatca tcgcgcgtga gatcctgccg
     3241 aacatgcgaa gttatgtcgc aatcagtttc gttctggcga tgacgcaggc aatctacgcg
     3301 caggttgggc tggtgtttct ggggcttgtg ccgctgtcag gccagaactg gggcgtgatg
     3361 atccagtacg gctggacgcg cggagcgatc tttcacaaat ccagcgtgta ttcaatcctg
     3421 gcgccgatca tggcgatcgc ggtgctgaaa ctctcgcttg tgtcgatggc gcggctcttc
     3481 gacgagatct tcaacccgag acttcaagga tgacggacga gctgatctca attcgagacc
     3541 tctccattgt ctaccgtggc cgcggaggct ccgcgcaggc gctgcgggag gtgagcctcg
     3601 acctctaccg cgacgaaacg ttggggctaa tcggcgaaag cgggtcaggg aagacgaccc
     3661 tcgcactggc cgtgttgcgc atgctcccca ctgcggcaca catcccgcac gggaagattc
     3721 tctaccatca ggatggccgt accgtcgacg tcttctcact ggatgcggag cggttgcgcc
     3781 gctaccgggg cgaacaggtg gcgatggtgt ttcagagcgc gctgagttcg ctgaacccag
     3841 tgttgcgcat ctgggatcag tttttggaca cggcgcgcgc ccacggtgaa tttgaccagg
     3901 tcagggtaca ggcaaggacg cgggaactgc tcagccacgt gcagctcgaa ccggggcgcg
     3961 tgctgggtgc attcccgcat gagctcagcg ggggaatgcg gcagcgggtg atgctcgcgc
     4021 tggcattact gcttcgacca cgggtggtcg tgctcgacga gccgacgacc gcccttgaca
     4081 tcctcaccca gcgcacgatt atcgacctgc tccgctcgct gcgtaaggaa tttggattct
     4141 caatgatgtt catctcccac gatctctcgc tggcggcgga actcgctgat cgagtggcga
     4201 cgatgtacgc gggcaggatc gtggaaattg gcacgacaga cgagctcttc tatgaaccac
     4261 ggcatcccta cacggttggg ctcctgcagg cggtccctac cgtacacggc ggacaccggc
     4321 cgctgcagtc gattcccgga tcgccccccg atctccagcg gcttccttct ggctgcaagt
     4381 tccacccgcg gtgtaagtac gcccggccta cctgcgcaga gactgaacct gatctgctgc
     4441 atgtatcgga cgggcaccga tcagcgtgct tcttctggga tgaagtgcgc aaggacgcgc
     4501 aagcgcgcaa tcgcggaagc acgcaaacgc gccccctcga tctcgaggcg agccagtgat
     4561 tatcctcgag cacgtcggcc gcgttttccg tagccgcgga cagatcctcc gggcggtaga
     4621 cgacgtctcc ctggagatcg gcgaaggcca ggtgatgagc ctggtcggcg agagcggcag
     4681 cggaaagacc acgaccggca aaatgatcgc gggactggtg acgccgagca gcggccgcgt
     4741 gctgttcgaa ggtcgcgata tccggacgct tgagggagat gcgcgcggcc agtaccgccg
     4801 cgctgtgcaa attgtccatc aggatcccta cgcgtgcctg aatccggtga ggacggtcgg
     4861 cgacacgctg gccgcgccgc tccgactgta tggcctcgcg gatggccggt cgcggaacgc
     4921 gggtatcggt gagcttctgg aacgcgtcga gctcacgccg gcgtcggcgt tcatcgataa
     4981 gtatccccat caattgagcg gcgggcagcg ccagcgtgtg gcggtcgctc gcgcgctgtc
     5041 ggtacggccg aaattcctcg tcgccgacga ggcggtctcc atgatcgacg tgtcgctcag
     5101 gatcagcctg ctcaacctgc tcctcaagct acgacaggag acgggtgtgg cgtttctctt
     5161 tattacacat gacctggccc tggcgcgcca cttcgggtgg gatggccgga ttgcggtgat
     5221 gtatctaggt cgggtcgtgg aggtcggtcc aaccccgggg gtgatcgagg atccccagca
     5281 tccctacaca cgcgcgctcc tggcggcggt gcccgaagcc gacccccgca tcacgcggac
     5341 caagccacgg ctgcgtctcc gcagccagga tcctccgagt ttgttgcacc tcccaccggg
     5401 ctgtgcgttt catccgaggt gtccctgggc ggaatccggg ctctgtgacg tgatccgacc
     5461 ggacctcacg cacgtacgcg cccaggaggt tgcctgccat gtcgcggcgc gggagggcgc
     5521 acttccggaa ctggctgaaa acgtgtgact ggatcgacga tgcatccgca gctcgcccgc
     5581 gcagcattaa gaattggggt gttcattgcg ctcacctccg gcgtactgac gttcatcgag
     5641 cctcgaggca gtcctgaaaa agccattagt ctctggacat tgctggccgc gctactgttt
     5701 ctcgccgtca ttgttgcgcg tatgcgtcga gtctaaagac ggagtacagt tgtgggtgcg
     5761 gaactactca aatgtgtctt tgggaggtgt gtacgatgcg taaggccatg acgatcatcc
     5821 tgacagcgct gttgctcgga ttttcaatcc tcccgcacga tgcgatgggg gcgccggcag
     5881 gcagcgtctt ccagggtgca tggccatacc aggcgccgcc ggcggggcac tacaacagtt
     5941 tcatccccgg atatctcata ccgtttgtga acccatacaa cgatctcgtg gagatgccct
     6001 tagccttcta cctgtggcag aagaacgaat acattcccgt gctcgcagcg cagtggaagg
     6061 cggaggccgg cgggcgagct ttcacggtgt cgttgcggcc cggcgtgcgt tggtctgacg
     6121 gaagcgcgtt cacttctgcc gatgtggcct caacatttta catcggctac cttttcggct
     6181 ttctcgcctg gcgaagcatc agcggcatcg aaacgcccaa cgaccaaacg gtcgtctttc
     6241 ggttcacaac ccccgcgtcg atcgcgctgc gcacgatcct gcgcgtcaat attcgcccac
     6301 ggtcggtgta cggagcgctg gccgatcggg cgaagcctta tgctgagcag cgcatggcgc
     6361 tgactgatga gcccgtccga actctcactg acgaagtccg ccgcttccgt ccccgagaga
     6421 gcgtggccac gggcccgttc cggatcgacg catcctccat cacagaggct cagctcaccc
     6481 tggtcaagaa tccgacgtcg ttctcggcga atcaggtgca gttcgatcgc atcgtattgt
     6541 tcaatggtga aacgccgcag attacgccgc tggcgctgtc gaagcagatc gactacgcca
     6601 cgcacgcatt cccgccggca actgacaggc agtttcagga actcggagtc cgggtgctcc
     6661 gcgctccggt atacagcggt ccggctctct tcttcaacca ccgcatctcg ccgctgaacc
     6721 gcaaggaggt ccggcaggcg atcgcatatg cggtgaatcg cgatcagagc ggcaccgtgg
     6781 ctctcggtgc gtccggacgc ggtgtcaagg tactgaccgg attcagcgac aatctcgccg
     6841 acaactggct gaccgccgac gtgaagagca aactcaattc ttaccgctac gatccgccgc
     6901 gcgccgcaca gatcctgacc cgcgccggct tcaagaaggg cacggatggg gtctgggtct
     6961 ccgacaaggg cgagcgcatg gagtacgaac tgagcgtccc ccctgaattc gcggattggt
     7021 cggcggccgc cgaggaagtg gcggcacaac tgactcgctt cggcattcgt acaaccgtgc
     7081 ggggcgtgcc gaaccctcag cacatcctga atgtcaatga ggggaggttt cagatcgccc
     7141 tgcgtacttg gggcgctgga aacccgcatc cattctttgc cttcagtcag gacctgttca
     7201 ttcacaacta tgtcgtgaac gcggttacac gcgggatgga tttcagcttg cggcagaaga
     7261 cggacgcgat gggcgaggta gacctcgcat caatggtgga ctcatccggg gccgggacca
     7321 acctcgcgcg ccagaagtcc gcagtatcgc gcctggcgct ggcgttcaac gaactgctgc
     7381 cgatcatccc gctgttcgaa cggtacagca acagcccggt agccgaaggc ttgcgggtga
     7441 ccgggtggcc cagagacgac gatccgatct acaagaatca tcttggcacc gacagtttcg
     7501 tgaccattat gatcttcaac ggaacgctgc gaccggcggg gcggtgactg tagcgctgcg
     7561 ctcggtcacg ccccgcgaag cgggtctgga ctcggatcgg gtccgaacta ttgaggattt
     7621 gatcgacgag ggtatccgca cgggtgtgtt tcccggcgct gttgccctcg ttgcgcgagg
     7681 agatacggtg ggctggcaat cggcgcgggg tgatgcgcag atcacccccg aacgccgccc
     7741 cgccgccctg gacaccatct atgaccttgc ctcgctgacg aaggtgatcg cggcactgcc
     7801 ggtcgcgctt cacgtgtggc aagaggggca actggatctc gacgctcgca tctcctcggt
     7861 gatcacggca ttcaccggtc cccacaagga acgggtcacc ttccgccacc tgcttgcgca
     7921 cacgtcgggg ctaccttcat ggcgcgcgct ctacttgaag gcaaaaagcc gcgagggcgt
     7981 gcgcgacgaa atctgccgca ccccgcttca ggccgagccg ggtgccgcgg tcgagtactc
     8041 ggacctcggt attttgctgc taggttttgc gttggagcac atcaccggcc gccgcatcga
     8101 tgaccttgtc ggggaagtcg tgatcaggaa actcggactg cgtgacacga tgtacaatcc
     8161 gccgtcgttt ctccacgcgc gatgcgcggc caccgaagcc ggccatggct acgagcgcga
     8221 gaaggtcgga gagacaggca gggggtttcg gtggcgcggt accgtcttgc agggcctggt
     8281 gcatgacggt aacgcgcact acggcatggg tggcatcgcg ccccacgcgg gattgttctc
     8341 taccgcctgg gaagtggcta ccgtcgcccg gcagtggctg cgtcccggtg aactggtcgg
     8401 cgccgctgca cttcgcgaag ccaccgccga tcagcgtcac ggcgcaaaag gttacccgcg
     8461 agggttgggc tgggtgctgc atcatgaagg aacgttcttt gaagcgctgg gcgcacgatc
     8521 gtttggccat accggattta ccggtacatc attggcgatc gatcccgatg aagagctcat
     8581 agtcgttctt ctcaccaacc gagtgcatct cggtggcggg aacgtaaaga ttcaggattt
     8641 ccggcctcgc tttcatgcgg tggtccgggg ggcggttctg tgacgcttct cgtcggtgtc
     8701 gactctggag cgacaaatac ccgggccttg gcggctcggg aggatggcac tccggttggc
     8761 agcgcctccg ctggaccagg aaatcctcac aacattggac ccggccctgc ggccacagag
     8821 atcgcgcgtg cgatccgcga ggcagcttca ggagaaactc cggatgttgt tgtcgcaggt
     8881 gtcgcggggc ttgaagaccc ggaactcaag cgagagcttc ggtacgcgct cgaggctctg
     8941 cgaccggccc aagagatcca cgtgtactcc gacgcggcgg tcgcgctcga tggcgcgttc
     9001 ctcggtggcc cgggcgtgat cgtcatcgcg ggcacgggat ccgtcgcctg gggtcggaac
     9061 gagaagggtc aagaagccag agcgggtggt tggggccaca ttattgacga cgcaggcagc
     9121 ggattcgacc tcgggcgcca ggcgctggcg gcctcgcttc gcgcgcacga tggtcgcggg
     9181 ccgcagacgt cgctcacgag acgactcacc gaagcgctgg gagtagaagg gccgttggac
     9241 ttcccgggcg ccgcgcggcg gctggatccc tcccgcatgg cctcgctcgc gcgcgtggtg
     9301 atcgaggccg ccgacgaagg cgatctcgtt gcccgcgcaa ttgtgagaaa tgcggcgggt
     9361 cacctcgctg agatcgccac agcggtgtgg aagaagctca atttgagccg tgggcatccc
     9421 gtagctccgg tcggcggatt gttcagtcat gcgcggctgc gcaaagcgtt tgcttcatca
     9481 ctcgcccgcc ggtgtccgga ggccgaagtg cggccaccga ggcttccacc ggtcgccggt
     9541 gcggtgtgga aagccgctgt cctcaaacgt cttgctccag acgaggcgat ggtgatgcag
     9601 ctcgaccgat gggcccagac cgccgccgag acccgatgaa cctgcgcgat gagttcggcg
     9661 cgctcagcac agagcagcgc aatgcaaagt cgatgcaact tgacacgatg agcacggcgc
     9721 agatcctaga gctcatcaat gatgaagatg gcacggttgc gggcgctgtc cgccgggcgc
     9781 taccggagat tgtgcgcgcc accgagctcg tggggggatc cattcgctcc ggcggccggg
     9841 tcttttatgt cggcgccggc accagcggcc ggatcggatc tcttgacgcg tccgagtggc
     9901 ggccgacgtt tgggaccgat ccggatttgg ttcaggcgat catggcaggc ggggcttcgg
     9961 ccaccgtcga cgctgcgtca ggattcgaag atgatgctgc actgggcgcg cgcgatgtga
    10021 caggtcgcgg cgtgcgtcgt ggggacgtgg tcgtcggcct ggcggcaagc gggaggacgc
    10081 cgtacgcgat cggcgcgctg caggccgcgc gtgccgccgg cgcggcgacg gtcgggatct
    10141 gctgcaaccc cgacgcgccg atgaccacgg tggttgatgt ggcgatcgtc gtggtcgtcg
    10201 gcccggaagt cctgactgga tcgacgcgca tgaaggcggg cacagcccag aaactgattc
    10261 tcaacatgat cagcactgcc gcgatggtga gagggggcaa agtttacagc aatctcatgg
    10321 tggacatggc tgcgggaaac gagaagctcc tcgaccgagc gcgccgcatc gtggcagaag
    10381 ctgctggggt tccggtcggc gaagcggcga aggtgcttga agcagccggg aaccgcgtga
    10441 aacccgcaat cgtgatggct gtacggggtt gcaatttcga cgaagcgcaa cagcgtttgg
    10501 agcgcgcggg tggcttcgtg cggaaggcgc tcgagtgact gcagggtgac gatggcactc
    10561 cattcctggc cgacgacaaa gagccccctc cgcgtcatcg gcctgatctc aggaacctcc
    10621 gccgacggca tcgatgcggc cgccgtcgac gtttctcgcc gggcagaggg cgttgatatt
    10681 cgtacgctgg cgactctcgc gagcgatttc aagccggagc agcggaagcg aatcctcgac
    10741 ctgtgccgcc ctgacgcggc gctcgaggcc gtttgcgcgg cgaacgtcga cgttgccgag
    10801 tggtttgccg atgctgcgct ggccgcaatt aagaaggttg gtagaccatc tgcagagttt
    10861 gatctgattg cctcgcacgg tcagacgatc tggcacatcc ccggccacag cacgatgcag
    10921 atcggtgaag gcgcggtaat tgccgagcgc acagggcttc cggtagtgag caactttcgg
    10981 gcgcgagata tggcggccgg tgggcagggc gcgccgctcg tgtcctacct cgactatctc
    11041 cttttccgcg atcctgcgcg cagccgggct gcgcagaatc ttggtgggat cgggaacgtc
    11101 acctggatcc ccgcaaactc ggcggcgggg gacgtgatct cgttcgatac cggaccggcg
    11161 aacatgctga tcgacggtgt cgcgcacctg actgctaagc gccagcgaga cgatggcggc
    11221 cagatcgcct cacgcggaca agtgcaggat cggttcctgt ccgagctttt ggcggatgcc
    11281 tactacgatc agccgcctcc caagaccacc gggcgcgaga agttcgggga cgcgtatgcc
    11341 aggcagattg tggaccgtgg ccgcaacctg aggatcaaag acgaggatct cctggccacc
    11401 gtcacgatgc tgacggcacg caccatcgcc gaggcgtatc gacgtttcct cggcaaggtc
    11461 gacgaggtca tcctgagtgg gggcggcgta cacaatcaga cgcttgtgca aatgatcgga
    11521 acggaactgg cgcccgctca ggtccggatc agtgacgcgt acgggctgga tgcggatttc
    11581 aaggaggctg ccgcatttgc cgtcttcggc gctctcaccg catggggcca gccctcaaac
    11641 ctaccggccg cgacgggcgc tcgccgcccc gtggttctcg gcgacattac tccctaggct
    11701 ctttccgtgc gcagttcgat atcgccggtc ttgatttccg ccattccgct cgcgcttgcc
    11761 atcggtgtgt ttggaacaat cttcggagcg gcttcgacgc cgctcatcgg tctggaactc
    11821 acgctggcgg cgtcgctcat cgttttctcc ggggcactgc aattcgccat ggttggactc
    11881 ttgctcgctg gtgcgccggt cccctcgcta ctggtgaccg ccgccctgct caacctgcgg
//
LOCUS       DBNGSB010000201         2558 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3519982, whole genome shotgun sequence.
ACCESSION   DBNGSB010000201 DBNGSB010000000
VERSION     DBNGSB010000201.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3519982"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..438)
                     /locus_tag="AC159B_05905"
     CDS             complement(4..438)
                     /locus_tag="AC159B_05905"
                     /inference="COORDINATES: protein motif:HMM:NF025887.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3377313.1"
                     /translation="MRFPSTAVLLLSVCALAACGEANREASPPPIQLEEEWGAALVRR
                     DTLAFGRLLAPGFVYTENALVMDRMEVIKSVTGPDHVEWARNEGMKVHDFGDVQVITG
                     VLHLRGKGKDGPFDRRFQFTDTWQRRNGRWQVIAAQDYLIPK"
     gene            596..1090
                     /locus_tag="AC159B_05910"
     CDS             596..1090
                     /locus_tag="AC159B_05910"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006365431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HIR3377314.1"
                     /translation="MTSRRLLLMNTRKRIALIAHDNRKHDLLDWVRCNRGILMHHDLF
                     ATGTTGSILSAELGLDITRFASGPLGGDQQVGAGIVEGRIDFVVFFSDPLEPHPHDVE
                     VKALLRVAVVYNIPVACNRATADFMVSSPLMKEEYRRLTMGIEDQLVRWVQPVQRSAL
                     PISE"
     gene            1199..1795
                     /gene="pnuC"
                     /locus_tag="AC159B_05915"
     CDS             1199..1795
                     /gene="pnuC"
                     /locus_tag="AC159B_05915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HIR3377315.1"
                     /translation="MDPVELVAVVMTLVGIVLTIRQKISCWPVAIVGVLAYFVVFARA
                     RLYADAALQPIYVVQNLYGWWYWSHGGERGHGQAPIVVLPWRRRLAWLAAIAVTSLIV
                     GAGLARWTNAAAPYADAALSTTSLAANWLLAKKVLENWWLWIAVNIGYVILFWKKSLL
                     LSAGLYAVLLALAATGYIEWQRSRARQIEAYRPGLWIP"
     gene            complement(1768..2283)
                     /locus_tag="AC159B_05920"
     CDS             complement(1768..2283)
                     /locus_tag="AC159B_05920"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017316301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3377316.1"
                     /translation="MNEIRLETERLILRKYRLSDFEDHFKLCADPDMMRYLIGGKPMS
                     RLEAWRHMAFLVGHWELLGYGYFAAEEKASGRFVGRIGFTNPEGWPGFEVGWSIAPEF
                     QGRGYATEGGRFLLEYAFNEMGKDHVIRVIHPDNKPSIRVAERLGEKLEGQTEVDGTK
                     MLIYGIHKPGR"
BASE COUNT          496 a          791 c          734 g          537 t
ORIGIN      
        1 ggtctacttc ggaatcaggt aatcctgggc ggcgatgacc tgccaccgtc cgttgcgacg
       61 ctgccatgta tcagtgaact gaaaccgccg atcgaatggg ccgtccttcc cttttcctct
      121 caggtgaagc acgccggtga tcacctgcac gtcgccgaag tcgtgaacct tcataccttc
      181 attgcgcgcc cactcaacgt gatcaggccc ggtgacgctc ttgatcactt ccatgcgatc
      241 catgaccaga gcgttctcgg tataaacgaa gcccggggcg agcagacgtc cgaatgcgag
      301 ggtgtcgcgt cgtaccagag cggcgcccca ttcctcttcg agctgtatcg gcgggggaga
      361 cgcttcgcga ttcgcctccc cgcacgccgc gagtgcgcaa actgagagga gcagtacggc
      421 agtcgaagga aatctcatct gtaatctcct cgatctgagc tattcggtta atgcaccttc
      481 ccgccctgcg ctgcaagcct ctggcctcgg aaacgttact caacccgaac cgtttggtga
      541 cggtatcaac gcacggcaac cgtaggctga gtcgtatcct gaatggagac gcagcatgac
      601 gagccgccgt ttattgctga tgaacacgag aaagcgcatc gcgctgatcg cccacgacaa
      661 ccggaagcac gacctcctcg actgggtccg ctgcaaccgt ggcatcctga tgcatcacga
      721 cctgttcgca accggcacta ccggctcgat actgagtgcc gagctgggtc tcgacatcac
      781 ccgatttgcg agcggcccgc taggtgggga tcagcaggtg ggagcaggaa ttgtcgaggg
      841 caggatcgac ttcgtggtct ttttctcgga tcctctcgag ccgcatccgc acgacgtgga
      901 agtcaaggcg ttgctccgcg tggccgtggt ttacaacatt cccgtcgcgt gcaaccgtgc
      961 gacggccgat ttcatggtct catccccgct catgaaagag gaataccgcc ggctgacgat
     1021 gggaatcgag gatcagctgg tgcgctgggt ccagcccgtt caaaggagtg cgctccctat
     1081 atctgagtaa tccgggtccg gaagctaggc atccgttgct gcgctgcaat tcctcccccg
     1141 ctctgggaaa aggatagcgg ccgacaagtc aacggaattc gactacattc gggccctcat
     1201 ggatccggtc gaattagtcg cggtagtgat gacgctcgtc ggcatcgtcc tcaccataag
     1261 gcagaagatc agctgctggc cggtggcgat cgtaggcgtc cttgcctatt tcgtcgtgtt
     1321 cgcccgcgcg cgactgtacg ccgacgccgc gctgcagccg atctacgtcg tgcagaatct
     1381 ctatggctgg tggtactggt cgcacggcgg cgagcgcgga cacgggcagg cgccgatcgt
     1441 ggttctcccc tggcggagac gattggcctg gctggccgcg atcgcggtca catctctgat
     1501 agtaggagcg ggtctcgcgc gatggaccaa tgccgctgca ccctacgccg acgcggcgct
     1561 ctcgacgacg agcctggcag ccaactggct gctcgcaaaa aaagttctgg agaactggtg
     1621 gctgtggatc gcggtcaaca tcggctacgt gattctgttc tggaagaaga gcctgcttct
     1681 ctcggcgggc ctttacgcag tactgctggc gctggccgcc acgggttaca tcgagtggca
     1741 acgctcgcga gcccggcaga tcgaggccta ccgtcccggc ttgtggatcc cgtaaatcag
     1801 cattttcgtc ccgtctacct ccgtctgtcc ttccagtttc tcgcccaatc gttcggcgac
     1861 cctgatcgag ggtttgttgt ctggatggat cactctgatc acatggtctt tgcccatttc
     1921 gttgaacgcg tattccagga gaaaccgtcc gccttccgtc gcgtatcctc ttccctgaaa
     1981 ttccggagcg atcgaccatc ctacttcgaa tcccggccag ccttccggat tcgtgaagcc
     2041 gatacggccg acgaaccgac ccgatgcttt ctcctccgcc gcgaaatatc cgtagccgag
     2101 tagctcccag tggccgacca ggaaggccat gtggcgccac gcttccagtc gcgacatggg
     2161 cttgcccccg atcaggtatc gcatcatatc cgggtcggcg cacagcttga agtgatcctc
     2221 gaagtcgctc aaccggtatt tgcggaggat cagccgctcc gtctcgagtc ttatctcatt
     2281 catcgaccgt cacgcgcgta tgtattgagg attcatggcc cgcgatgaag aaattcatag
     2341 acagtaaacg tagattagat gaagtttgac gcagaagctg ttcaccagaa tcacgcaatc
     2401 gctacgagga ccgtttgatc caggcacaac aagggcagag cccttcgact ccattcgtgc
     2461 ccctggcacc agtcgtgccc ctggcaccag gcgtgcgccc tgcgccgagc gcctcagcaa
     2521 tctacgaggg attcaaggcg cagcggcacg agctgaat
//
LOCUS       DBNGSB010000202         3291 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_1067636, whole genome shotgun sequence.
ACCESSION   DBNGSB010000202 DBNGSB010000000
VERSION     DBNGSB010000202.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_1067636"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="AC159B_05925"
     CDS             complement(<1..670)
                     /locus_tag="AC159B_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR3377317.1"
                     /translation="MPDTIIVEKADGVMTLTLNRPDVLNAFNDTMSGEMQEVLRDAER
                     DASVRAIMITGAGRGFCSGQDLRDRVGTSSFSFVESLRRRYNPIIAKLISIEKPVLAA
                     VNGVAAGAGCSLALACDLRIASDGASFIEVFARVGLVPDSGSTYLLPRLVGLGKAFEL
                     CYLAEPLSAADAERIGLVNWMVPAHQLLEKARAIAARLSAGPTKAYGLIKRALYHNLY
                     SDVET"
     gene            complement(741..1514)
                     /locus_tag="AC159B_05930"
     CDS             complement(741..1514)
                     /locus_tag="AC159B_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR3377318.1"
                     /translation="MAYENIVVTAEDRVAVVTLNRPQVLNALNRATMDELVDALEGYD
                     RDETIRCVILTGAGRAFAAGADINEFRGATPVTMLNQYRFQQWERIRKIGKPIIAAVN
                     GFCLGGGCELAMLCDIILAAEAARFGQPEITLGIMPGAGGTQRLPRAVGKHRAMELIL
                     TGKQISAQVAHNWGLVNRVVPGEMLMDEARALAREIAEKAPVAVRLAKEAILKSLDSP
                     LETGLDHERKLFYLLFSTQDKDEGIEAFLSKRKPEFKGR"
     gene            complement(1522..2403)
                     /locus_tag="AC159B_05935"
     CDS             complement(1522..2403)
                     /locus_tag="AC159B_05935"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR3377319.1"
                     /translation="MAYPHSQSGSAFKFRQILYEKHPPVARVTINRPEVYNALNFEAL
                     REMSRAFEDASWDDAVAVLVLTGAGDRAFSTGADLNEQEQFLRRPSDYWKWMGVFIEA
                     QERLRNIGKPSIARLNGMVVGGGNEFNIACDLAVAADDIVIRHVGPARGSVPAAGATQ
                     WLPLIVGDRRAREIIFLCEEIPVSKALEWGLVNQVVPRAELDRAVDALARKLTEKLPE
                     VTRYTKQQLNFWRDLSWHLTVGHARDWLTLHAGSEEVQEGLEAFRQKRPVRYEKFRRG
                     KPEARKRASAQAKRRTR"
     gene            complement(2408..2710)
                     /locus_tag="AC159B_05940"
     CDS             complement(2408..2710)
                     /locus_tag="AC159B_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009711844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HIR3377320.1"
                     /translation="MVKLVALFKKPADQESFDRHYADTHIPLVRKWPGLRKVETGKIT
                     GAIGGETPYYQVAEMYFEDMDALKAALRSPEGRAAGADLQSFAPGLVTMLYVNVSH"
     gene            complement(2779..>3291)
                     /locus_tag="AC159B_05945"
     CDS             complement(2779..>3291)
                     /locus_tag="AC159B_05945"
                     /inference="COORDINATES: protein motif:HMM:NF016895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR3377321.1"
                     /translation="AVIGLFAIAVLIGYVGGRSFLATTASAPRQLVRPVPRPDPQPPP
                     PLAPVQPEPSRPAPTPTPQAEPEPEPTPQPAPAPQPAPAPQPAPRPQPPQAAPPSGAV
                     YRVQVGAFLKRENAAARAEELRGAGFEAYINQSGGLFKVQVGSFGDRDNAVQLAQQLR
                     DRGYEVLILP"
BASE COUNT          586 a         1055 c         1001 g          649 t
ORIGIN      
        1 cggtctcgac atcactgtac aagttgtggt aaagtgcgcg cttgatcaaa ccgtaggctt
       61 ttgttggtcc ggccgacaga cgggccgcga tcgcgcgtgc tttctccagc agttgatgcg
      121 cggggaccat ccagttcacc agtccaatgc gctccgcatc tgcggcagac agcggctcag
      181 cgaggtagca tagctcaaac gctttgccga gccccaccaa gcgaggaagc aggtacgtgc
      241 tcccggaatc cgggacgagc ccgacacgcg cgaacacttc aatgaacgag gccccatcgg
      301 aggcaatccg caggtcgcag gccagcgcca ggctgcaccc tgcgccggcg gcaaccccgt
      361 tcacggccgc gagcaccggc ttctcgatcg agatcagctt tgcgatgatc ggattgtacc
      421 ggcgccgcag gctctctaca aacgaaaacg aggaggtccc gacccgatcg cgcaaatcct
      481 gtccggagca aaaaccacgg ccggcgcctg tgatcatgat cgcccgaaca gaagcatcgc
      541 gctcggcgtc cctgagcact tcctgcatct ccccggacat cgtgtcgttg aacgcgttca
      601 acacatcggg acggttcaac gtgagcgtca tcacgccgtc tgccttttcc acgatgattg
      661 tgtcgggcat taacccctcc aattacggtc ggaggtcgtc ggtcgtggca actgctcttc
      721 ctgcgaccta tgaccgcctt ttatctcccc ttgaattcgg gcttccgctt gctaaggaac
      781 gcctcgatgc cctcgtcctt atcctgtgtg ctgaaaagca agtagaacag cttgcgctcg
      841 tgatcgagtc cggtctcgag tggcgagtct agggacttca gtatggcttc cttggcaagc
      901 cgcactgcga ccggcgcctt ttccgcgatc tctcgcgcga gcgccctcgc ctcatccatg
      961 agcatctcgc ccggcacaac tctgttcaca aggccccagt tgtgcgccac ctgcgcggag
     1021 atctgcttgc cggtgaggat gagctccatc gcgcggtgct ttccgactgc ccgcggcagt
     1081 cgctgcgtcc cgcccgctcc cggcatgatt ccgagggtga tctcgggctg gccgaagcgc
     1141 gcggcttcgg ccgccagaat gatgtcgcac agcatggcga gctcgcatcc gccacccagg
     1201 cagaacccgt tgacggccgc gatgatcggc ttgccgatct tgcggatgcg ctcccactgc
     1261 tggaaccggt actgattgag catcgtcaca ggggtcgcgc cgcgaaactc attgatgtcg
     1321 gctccggcgg caaatgcccg accggcgccc gtaaggatga cgcagcggat ggtttcgtct
     1381 cggtcatatc cttcgagcgc atccaccagt tcatccattg tggcccggtt aagcgcgttg
     1441 aggacctggg gacggttcaa tgtcacgacg gcgacgcggt cctcggcggt gacaacgatg
     1501 ttctcgtagg ccatgatccc ctcagcgagt ccgccgtttc gcttgcgcgc ttgcgcgctt
     1561 gcgcgcttcc ggtttcccac ggcggaactt ctcgtaacgc accgggcgct tttgtcgaaa
     1621 tgcctcgagc ccttcctgca cttcctccga tccggcatgc agggtgagcc agtcgcgtgc
     1681 atgaccgacg gtgagatgcc agctgaggtc tcgccagaag ttcagttgct gtttcgtgta
     1741 gcgcgtgacc tcgggcagct tctccgtcag tttccgtgcc agcgcgtcga cggcgcggtc
     1801 gagctccgcg cgcggcacca cctggttcac gagaccccac tcaagcgctt tgctcaccgg
     1861 gatctcttca cacaggaaaa tgatttcgcg cgctcggcgg tcaccgacga tgagggggag
     1921 ccactgagtc gctccggccg ccggaacgct tccgcgcgcc ggtccgacat gccggatgac
     1981 aatatcatcg gcggccacgg cgaggtcgca ggcgatgttg aattcgttgc cgccgccgac
     2041 caccatcccg ttcagacggg cgatggacgg cttgccgatg ttccggagtc gctcctgagc
     2101 ctcgataaag acccccatcc acttccaata gtcgctcggg cggcggagga actgttcctg
     2161 ttcattgaga tccgcaccgg tagagaaggc gcggtcgcct gcgccggtga ggacgagcac
     2221 cgctaccgca tcatcccacg acgcatcctc aaacgcgcgg ctcatctcac gcaacgcctc
     2281 gaaattcagt gcgttgtaga cttctggccg gttgatcgtg acacgagcga ccggcggatg
     2341 cttctcatag aggatctgcc gaaacttgaa cgcggatccg gactgggaat gtggatatgc
     2401 catgcgtcta gtgcgagacg tttacgtaga gcatcgtgac cagaccgggc gcaaacgatt
     2461 gcaggtccgc tccagccgcc ctgccttcgg gagatcgcag ggccgccttg agcgcgtcca
     2521 tgtcttcgaa gtacatctca gcaacctgat aatagggcgt ctctccgccg atggcacccg
     2581 tgatcttccc ggtctcgact ttccgcaaac cgggccactt tctgacgagc gggatgtgag
     2641 tgtcagcgta gtgacggtcg aagctctcct gatccgcagg ttttttgaac aacgcaacca
     2701 gcttcaccat tggaaccctc ccgtgaatgg tgaatggtac tgcagcggtc ctgattcact
     2761 attcacgatt caagtcagtt aaggtagtat cagcacctca tatcccctgt cccgtagttg
     2821 ttgtgcgagt tgcaccgcgt tgtcacgatc gccgaacgag ccgacctgga ctttgaagag
     2881 acccccggac tggttgatgt aggcctcgaa cccggctccc cgaagctctt ccgcacgcgc
     2941 cgccgcattc tcgcgcttga ggaacgcgcc aacctgcact cggtagaccg cgccgctcgg
     3001 gggagcggcc tggggaggtt gcggccgtgg cgccggctgc ggcgcggggg caggctgcgg
     3061 cgcgggggca ggctgcggcg tcggttcggg ttcgggctca gcctgaggtg taggagtggg
     3121 tgcgggtcgc gatggctccg gttggaccgg tgccagtgga ggcgggggct gggggtccgg
     3181 tcgtggcacc gggcggacca attgccgcgg cgccgatgca gtcgtcgcta aaaatgagcg
     3241 accaccaaca tatccgatca acacggcaat ggcaaagagt ccgatcaccg c
//
LOCUS       DBNGSB010000203         9834 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_1987441, whole genome shotgun sequence.
ACCESSION   DBNGSB010000203 DBNGSB010000000
VERSION     DBNGSB010000203.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 9834)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9834)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9834
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_1987441"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="AC159B_05950"
     CDS             <1..425
                     /locus_tag="AC159B_05950"
                     /inference="COORDINATES: protein motif:HMM:NF012784.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HIR3377322.1"
                     /translation="LKQILDDPWHKVRERYRVGESVKGKVVRTVPSGAFVRLEDLDAF
                     IPISELAERRVNKVDEVVKPGDSVEAVVMEIRPDERRMTLSIRKMERDAERKRVKEVL
                     KSQDDGGRVTIGDLAGELLRQAVSPVPGDGESEKGEGS"
     gene            561..638
                     /locus_tag="AC159B_05955"
     tRNA            561..638
                     /locus_tag="AC159B_05955"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:596..598,aa:Pro,seq:cgg)
     gene            complement(706..1041)
                     /locus_tag="AC159B_05960"
     CDS             complement(706..1041)
                     /locus_tag="AC159B_05960"
                     /inference="COORDINATES: protein motif:HMM:NF039183.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HIR3377323.1"
                     /translation="MSPDAFISRQLWRWYIVVFIANELDLVFTYFGLGQGFLQEINPL
                     LRPHIYTWWPITIKVVALAGLALGIAAAVHASFARKRRVLKVLRGVAAVYGIVLILHL
                     VTILRAFAG"
     gene            1150..1626
                     /locus_tag="AC159B_05965"
     CDS             1150..1626
                     /locus_tag="AC159B_05965"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: protein motif:HMM:NF016085.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016891 - RNA endonuclease activity
                     producing 5'-phosphomonoesters, hydrolytic mechanism
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease FAU-1 family protein"
                     /protein_id="HIR3377324.1"
                     /translation="MKEIKHKLNGERMQFECKLLERSERRAVLLYPIPEGVQVGDLAL
                     PAGTTTYAHYWTDGPFNVYHWVAPDGNTLAYYVNLGDTVAFHEDGVEWRDLAVDILRT
                     PDGRTQVLDTDELEDVPPGLRRKITAIRDQVLAELDEVVNEVAAATARLRREDSER"
     gene            1707..2183
                     /locus_tag="AC159B_05970"
     CDS             1707..2183
                     /locus_tag="AC159B_05970"
                     /inference="COORDINATES: protein motif:HMM:NF024139.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR3377325.1"
                     /translation="MMKDLRAWYTYLEEIARKATSSDASPSRPAQSRVFRIPPADELL
                     IAGTTPSGRLRPQPLPIIPSSDTNAPRRPGRRPLTETREEIILRLLDPEMTLHEAAAL
                     LNLSKATVRRYTTIGKLHCSRTAGGQRRFRLSDLLTFLESGGGRRLRPKTKTEVAF"
     gene            2299..3441
                     /locus_tag="AC159B_05975"
     CDS             2299..3441
                     /locus_tag="AC159B_05975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3377326.1"
                     /translation="MFSESYLPRISGVTHSLAAFVAALRAKGHHVVVVAPAFPGHVDD
                     DPDIFRLPSIRIPQQRDFPLMNVLALSTWREVGRLDLDLIHTHSPFLVGRLAEHLARR
                     RRVPLVFTHHTIYDEYLHYVPLLPPALTRPVTRRYVTAYANQCDLVIAPSSMIAARLR
                     SLGVRSRVEILPTGVVDEALTKSLDPSWVRPAFGIPAGRALLVTASRLGREKSIDLLL
                     QMFAVVVKQRDAMLLIVGGGPEEQELKDLAGRLGVADRTVFAGQQPHQRTLECVSAGD
                     IFVFASQTETQGLAVVEAMAAGKAVVAVGAGGVTDAIENGVSGELVPASVDNMAGRVL
                     HLIDHPVARAGLGARAREASRRFSPEAAAGKLVHLYQSLKRAEPAG"
     gene            3608..7204
                     /gene="smc"
                     /locus_tag="AC159B_05980"
     CDS             3608..7204
                     /gene="smc"
                     /locus_tag="AC159B_05980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HIR3377327.1"
                     /translation="MYLRRLELQGFKTFAERTELEFTSGITAIVGPNGSGKSNVFDAI
                     RWTLGEMSFKSLRSGRMDDIIFSGTEARRAMGQSEVSLTINNESGILPVDYAEVTVTR
                     RANRGGEGEYFINDTVSRLRDIQMMFLGTGLGGRSYSLIGQGQVDGVLNAGPEDRRVL
                     LEEAAGLARYKRRRREAERRLQHAAVNLQRVGDVLAELERQLEHLRGQAKAATEYRAY
                     TTEVRDLELALQVDDTRRIVSGLKRIASQTEAVRHDLHETAAQATVTGTAIDRDRART
                     AELARAWEDTQRSLLQIVEDLSGRGSALQILRERLKHNDDRRERVEAEIARITEEISQ
                     AEQALTVLQAQAVEIRTRREALLEHLHFAEQSRTQTGVRREESEARLEAVQSEINDLN
                     AARTRSLHDLAYRDAKAASLAEQIDAHRTRIKELNDSVGEATAQVQQGENSLDLIQAR
                     REESLSHLAKAREQQRMNTSALEALDRDARDLLAEQQVVQSTLAFLEDMHRQLAGYEQ
                     GVKEILLAKRDQPGKFDGIKYPVAELLQVAPAHRPAIEAALGRRLFSLIAGTVDDVKD
                     GVSYLRGNGQGSATFLPVELVSPRPAPAVPVAANVIGRAADLVQLNNGARHVVEALLG
                     DVIVVTDLDAAVQQRRSGFVGRIATLHGEVLSPDGVISVRGKPDGEAGLLGRRDQIRS
                     LAERRRDLEERLAVLVSRRDGLSATLRDSETAVASGETQVADVDRKIAEQQAALVVLR
                     SSLAAIPAERTTVCDALAVAEAEHGREDTDAGRLRIVLELISKTLAERDRSAEVLDGE
                     IKQMIESSQTASGQLTDVRVSLAELAASLEALHARVDEHARDGAVFATRREELHGELT
                     VLHGERHLLQHSLEEARGALQELVELQERARAQLTTWEEERTVLQQRLADSEAVWRQI
                     QDSLRGFEEQAHRLEVRHAQVETELTAAQRRISEEFGMNWEEVREARLPVGRDEAQGR
                     IQALRGLLGAMGPVNLRAVDEFQSVSTRVEALLMHTKDLDQARAAITKLIAELDAVLR
                     VRFTQTFAAVNEEFDRLFVRLFAGGRARLTLVDPEPGPNGEVNANAEPGIEIEAQLPG
                     KKMRSLSAYSGGERVLIALSLIFAMLRVHPSPFCIFDEVEAALDDANTKKFTTLLREL
                     AERTQVLIITHNKGTMEAADILYGVTMEVPGVSKMISMRLARQETPESASVV"
     gene            7219..8118
                     /gene="ftsY"
                     /locus_tag="AC159B_05985"
     CDS             7219..8118
                     /gene="ftsY"
                     /locus_tag="AC159B_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIR3377328.1"
                     /translation="MWNFYDRLKTGLTRTRDAFASRVDQVLAQPLSPAVFDDLESALI
                     QADVGVIATSVIIDRLRARRDVRDTPSVRQALRAIIIELLGRSEPLRIEPPPAAVIVL
                     GVNGVGKTTTIGKLAYRLSRDGRRVLLAAADTFRAAAIEQLAVWAQRVGADVVRHAQG
                     ADPAAVVFDAIAAAKARGIDVLIVDTAGRLHTKVNLMEELKKINRVVARELAGQPVER
                     LLVLDASMGQNAITQARTFKDAVDVTGLVLAKLDGTARGGAVIAIAHELRLPVKFIGI
                     GEALDDLQPFEPREFADALLPGA"
     gene            8225..8443
                     /locus_tag="AC159B_05990"
     CDS             8225..8443
                     /locus_tag="AC159B_05990"
                     /inference="COORDINATES: protein motif:HMM:NF016205.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HIR3377329.1"
                     /translation="MHIVRLVEVYGGLLTARQLRQLRMYYLDDLSLGEVADQLDVTRQ
                     AVFDSLKRSLDKLNRLEGELHLVRRPRL"
     gene            8446..9060
                     /locus_tag="AC159B_05995"
     CDS             8446..9060
                     /locus_tag="AC159B_05995"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017026.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3377330.1"
                     /translation="MEHYFSTRPQVVSRPREVHAVLRGRKWTFLTDRGVFGHKGVDAG
                     TRLLIDTMVVRPSDDVLDLGCGYGPVGIVAATLAVQGRVVMIDVNERAVALAAENARR
                     ARLANVEVVAGDGLGQLAGQKFDVIATNPPIRAGREALRNLFRDAGDHLRDQGRFYFV
                     ARTAQGAKTLAAVVDAIIGNVQELAKGGGYRVYEATKKAEKLES"
     gene            9137..>9834
                     /locus_tag="AC159B_06000"
     CDS             9137..>9834
                     /locus_tag="AC159B_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012662.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HIR3377331.1"
                     /translation="MFENLQGRLQKIITQLTGRGVLSDADVDAALREVRLALLEADVN
                     FKVAKTFIDRVKAAAIGQQVTQHLAPGQQVVKLVHEELVRLLGGASHELRFADRSPTI
                     VLLVGVHGAGKTTTAAKLASRLKRRGRQPLLVATDLRRPAAVTQLQLVGTQVGVPVFS
                     QPGATDPIGVAQASLIEAERSGHDVVIVDAAGRQHADDELMAELRAMRDVLHPDNVVL
                     VLDAMTGQDAVAMA"
BASE COUNT         1894 a         2957 c         3184 g         1799 t
ORIGIN      
        1 gattgaagca aatcctcgat gatccctggc acaaagtgcg agaacgttat cgcgtcgggg
       61 aatctgtaaa gggcaaggtc gttcgaacgg ttccatccgg cgcatttgtg cggttagaag
      121 atctcgacgc gttcattccg atttctgaac tcgcggagcg ccgcgtgaac aaagtggacg
      181 aggtcgtaaa gcctggtgac agtgttgaag ctgtcgtcat ggagattcgg cccgacgagc
      241 ggcggatgac actgtcgatc cgtaagatgg aacgggatgc ggaacgcaag cgcgtgaaag
      301 aggtcttgaa gtcgcaggac gatggcggcc gcgtgacgat cggcgacctg gcaggcgaat
      361 tgctcaggca ggcggtctcg cctgtgcccg gggacggcga gtcggagaag ggggaaggaa
      421 gctaggctgg tctcacagat ctgtactgac tacacggtct gcccaatcca aaatgatcat
      481 gcacgcctgg tcgggcgtgt tttttgttgg agatcgtgta gattgtgcag attgtgcaga
      541 ttgtgtagac ttgtttgcgt cggggagtag cgcagcttgg ttagcgcgca gcgttcggga
      601 cgctgaggcc gccggttcaa atccggcctc cccgaccagt gtcgacgcgg gattcgtcag
      661 tatgaatccc gcgtcaattt ttgactcggg gaagattcga cggctttagc cggcgaatgc
      721 gcggaggatc gtgacgaggt gcaggatcaa gacgatgcca tagacggcgg cgaccccgcg
      781 taggactttc aggacccgcc gctttcgagc gaagctcgca tggacggcgg cagcgatgcc
      841 gagggccagg cccgcaagtg cgaccacctt gatagtgatg ggccaccatg tgtaaatgtg
      901 tggacgcagc agagggttga tctcctgcaa gaatccctgt ccgaggccga aatacgtaaa
      961 cacgaggtcc agctcgttgg cgatgaaaac gacgatgtac catcgccaca gttgcctgct
     1021 gatgaaagcg tccgggctca tcctcgtctg gtgggagtgg tgcccttcat tcgatgaacg
     1081 gcaccaggtt ccttgtcaga ggttttcccc aggtcaggca agtccaatct ccaggggcag
     1141 cacaagcgta tgaaagaaat caagcacaaa ctcaatggcg aacggatgca atttgaatgt
     1201 aaacttctgg agcgctcgga gcgccgtgcc gtgcttctct atcccatccc tgagggcgtt
     1261 caggtgggtg atcttgccct gccggcgggg accactacct acgcgcatta ctggacggac
     1321 gggccgttca acgtgtatca ctgggtcgcg ccggatggga acacgctggc ctattacgtc
     1381 aatctcgggg atacggttgc gttccatgag gatggggttg aatggaggga cctcgcggtc
     1441 gacatccttc gcacacccga cggtaggaca caggttctgg acaccgatga gctggaggac
     1501 gttccgcctg gcctgcggcg gaaaatcacc gccatccggg accaggtgct ggccgagttg
     1561 gacgaagtcg tgaacgaggt ggcggccgct accgcccgcc tccggcgaga ggattcagag
     1621 cgctagccgc gaaccccctt ctactagttc gcccaaacgc agcatgggcc ggaccggtca
     1681 ccgcagcatc actgacaccg gggcgaatga tgaaagatct tcgggcctgg tacacctatc
     1741 tcgaggagat cgcccgaaag gcgacctcct ccgacgcgtc cccctcgcgg ccagctcagt
     1801 caagggtgtt tcgcattcct ccggcggatg aactgctgat cgccggcacg acaccttctg
     1861 gtcgcctgcg cccgcagccg ctgccgatca ttccttcctc tgatacgaac gctcccagac
     1921 gccccggacg caggccgctc accgagaccc gagaagaaat catcctccgg ctccttgatc
     1981 cagagatgac cctccacgaa gctgcggcgc tcctcaatct gagcaaagcc accgtgcggc
     2041 gatacacgac gattggaaaa ctgcattgtt ctcgcacggc gggcggccag aggcgattca
     2101 gactatcgga tcttctgacg ttcctggagt ccggcggcgg acgccggctg cggccaaaga
     2161 ccaaaaccga agtcgctttc taaattgccg tcttcccagt ctccctcgac tgccccgtct
     2221 tccctgtcta ctggcatatt agactgggaa gactgggaag acagggtaga cggggtagac
     2281 gccgttttgc gtatcgggat gttcagcgaa tcctacctgc cgcgcatcag tggagtaacg
     2341 cattcgcttg cggcgtttgt ggcggcgctt cgcgcaaaag gacaccacgt cgtggtggtc
     2401 gcacctgcat ttcccggcca tgttgatgat gaccccgaca tcttccgtct gccgtcaatc
     2461 cgcattcccc agcagcgtga ttttccgctg atgaacgtgc tcgccctctc aacatggcgc
     2521 gaggtcggcc ggctcgacct agacctcatc catacgcact caccatttct ggtcggccgg
     2581 ctcgctgagc atctggcacg caggcgtcgc gtgcctctcg tctttacgca ccacacgatc
     2641 tacgacgagt acttgcacta cgtgcccttg ctcccccccg cactgacgcg cccggtcacg
     2701 cgtagatatg tgaccgctta cgcgaaccag tgcgatcttg tgattgcccc gtcttccatg
     2761 attgccgcaa ggttgcgttc gctgggcgtt cgatcccggg tcgaaatcct gccgaccggg
     2821 gtggtcgatg aggccctgac caaatcgctc gacccctcgt gggtccgacc cgcttttgga
     2881 atccccgccg gtcgtgcact cctcgtcacg gccagtcgtc tcggcaggga gaaaagcatt
     2941 gatttgctgt tgcagatgtt tgctgtcgtc gtaaagcagc gcgatgccat gctgctcatc
     3001 gtgggtggtg ggcctgagga acaggagctg aaggatctcg cggggcggtt aggggtcgcc
     3061 gaccgcacgg tgtttgcagg tcaacaaccg catcaaagaa cgctggagtg cgtgtccgcg
     3121 ggagatatct tcgtctttgc gtcccaaacg gagacccagg gattggccgt ggtcgaggcg
     3181 atggccgcag gcaaggcggt ggtcgcggtc ggagcgggcg gggtgaccga cgcgatcgag
     3241 aatggggtaa gtggggaact ggttccggcc tcggtcgaca acatggccgg acgcgttctc
     3301 cacttgatcg accacccagt cgcgcgagcg ggtctgggtg cccgcgcccg ggaagcctcg
     3361 cgccggtttt ccccggaggc cgccgcaggg aagcttgtgc atctttacca gtcactcaag
     3421 cgtgccgagc cagcgggcta gtatggcacc ctccaatgtg gcctgcgaat caggccggcg
     3481 gcgctgttga ggaggccgag cctgaggcaa ggccggtgga ttatgcacgt tgattgggtc
     3541 tggagccgag cgaggtcgag gccgggtgaa tcttgcttgg tgccgggtta aatagaggag
     3601 atcgatcgtg tatctgagac ggttggagct tcaagggttc aagacatttg ccgagcggac
     3661 cgaactggag ttcacgtccg gcatcacggc gatcgtcggc cccaatggca gcggaaaaag
     3721 caacgtcttc gacgcgatcc ggtggacgct cggggagatg agcttcaaga gtctccgcag
     3781 cgggcgcatg gatgacatca tcttcagcgg gaccgaagcg cgccgtgcca tgggccagtc
     3841 ggaagtctcg ctgacgatca acaatgaatc cggcattctt ccagttgact atgccgaagt
     3901 gaccgtcacc cgccgcgcca accgcggcgg tgagggtgag tactttatca acgataccgt
     3961 ctcccggttg cgcgacatcc agatgatgtt cctgggaact ggtctgggcg gccgctctta
     4021 ctcgctgatc gggcaggggc aggtcgacgg cgtgctcaac gcagggcccg aggaccgccg
     4081 ggtgctgctt gaagaggcgg ccggattggc gcgctacaag cgccggcggc gtgaagcaga
     4141 gcggcgactc cagcacgccg cggtcaattt gcagcgggtc ggggacgtgc tcgcagagct
     4201 cgagcgacag ctggagcacc tgcgcgggca ggccaaggcc gccacggagt atcgcgctta
     4261 cacgacagaa gtgcgggatc tcgaacttgc cctgcaggtc gacgacacac gacgtatcgt
     4321 gtccggcctc aaacggatcg ccagtcagac cgaggctgtc cgccacgatt tgcacgagac
     4381 cgccgcccag gccacagtca caggaaccgc gatcgaccgg gatcgcgccc gcaccgctga
     4441 acttgcgcgg gcgtgggaag acacgcagcg ctcgctgtta caaatcgttg aagatctctc
     4501 cggtcgcggg tcggcactgc agattttgcg ggagcgcctc aagcacaatg acgatcgccg
     4561 cgaacgcgtt gaagcagaaa ttgcacgtat cacggaagag atttcgcagg cggagcaggc
     4621 gctgaccgtt cttcaggctc aggccgtgga gatccgaact cgtcgtgagg cactcctcga
     4681 acacttgcat ttcgccgagc agtccaggac gcagacagga gtccgccggg aagaaagcga
     4741 ggcacgcctt gaggccgtgc agtccgagat caacgatctg aatgcggccc ggacgcgctc
     4801 gctgcacgac ctggcatata gggacgcgaa agccgcctcg ctcgcggaac agatcgatgc
     4861 gcatcgtacc aggatcaaag aactgaacga ttcagtgggc gaagcgaccg cgcaggtcca
     4921 gcaaggcgaa aactcgcttg acctgatcca ggccaggcgg gaggaatccc tctcgcacct
     4981 cgcaaaagcg cgtgagcagc aacggatgaa cacgagcgcc cttgaggcat tggaccgcga
     5041 tgcacgtgac ctgctcgcgg aacagcaagt cgtacagtcg acgctggcgt tccttgaaga
     5101 catgcaccga cagctggcgg gatacgaaca gggcgtgaag gagatcctcc tcgccaaacg
     5161 tgaccagccc ggcaagttcg acggcatcaa atatccggtg gcggaattgt tgcaggttgc
     5221 cccggcacac cgccccgcga ttgaagcggc gctgggccgc aggctatttt cgctgatcgc
     5281 aggaaccgtc gacgatgtca aggatggcgt gtcgtacctg cgcgggaacg ggcaaggcag
     5341 cgcgacgttt cttccggttg aactggtgag tccgcggccg gcgcctgccg tccctgttgc
     5401 ggcgaatgta atcggacggg ccgcagacct ggtgcagctg aataacggcg cgcggcatgt
     5461 cgtcgaagcc cttcttggcg acgtcattgt cgtcaccgat ctcgacgctg ccgttcagca
     5521 gcgacggtcg ggcttcgtgg ggcgcatcgc cacccttcat ggtgaggtcc tctcgccgga
     5581 tggcgtcatc agtgttcgcg gcaagcccga tggcgaggcc gggttgttgg ggcggcggga
     5641 ccagatccgc tcgctggccg aacgccggcg cgacctcgag gagcgcctgg ccgttctggt
     5701 ctcgcgccgt gacggcctga gcgcaacgct tcgtgacagt gaaacagccg tcgcatcggg
     5761 cgaaacgcag gttgcagacg tcgaccggaa gatcgccgaa caacaggcgg ctctcgttgt
     5821 gctccgcagt tccctggccg cgatccctgc cgagcgcacg acggtgtgcg acgcactggc
     5881 agtggctgaa gcagaacacg gacgcgaaga caccgacgcc ggccgcctgc gcatcgttct
     5941 cgagctcatt tcgaaaaccc tagcggaaag ggatcggtcg gcggaggtgc tcgacggcga
     6001 gatcaagcaa atgatcgaat cttcgcagac agcgtcgggc cagctgaccg atgtccgggt
     6061 ttccctggcc gaactcgccg cctcgctcga agcgctccat gcgcgcgtgg acgagcatgc
     6121 gcgtgacggg gctgttttcg ccacgcgccg ggaagaactc catggagagc tcaccgtgct
     6181 gcatggggag cgccaccttc ttcagcactc gctcgaagag gcccgcgggg cgctgcaaga
     6241 acttgtcgag cttcaggagc gcgcgcgggc gcaactcacc acgtgggaag aggaacggac
     6301 ggtgctgcag cagcgcctgg cggattcaga agctgtctgg cggcagatcc aggatagcct
     6361 gcgaggcttc gaggaacaag cacaccgtct cgaagtccgc cacgctcagg tggaaaccga
     6421 gttgacggcg gcgcagcggc ggattagcga ggagttcgga atgaactggg aagaggtccg
     6481 cgaggcgcgt ctgccggtag ggcgagacga ggcgcagggc cgcatccagg ccctacgcgg
     6541 attgctcggc gccatgggtc ccgtcaacct tcgcgccgtt gacgaattcc aatccgtctc
     6601 gactcgtgtc gaggcgcttc tcatgcatac caaggatctg gaccaggccc gcgcggcaat
     6661 cacgaagctg attgcagaac tggatgccgt gctgcgggtg cgcttcacgc agacctttgc
     6721 cgcggtcaac gaggagtttg accggctctt cgtgcggctg tttgccggcg gccgtgcgcg
     6781 gctcacgctg gtcgatcctg agcctggtcc aaatggtgaa gtgaacgcca acgcggagcc
     6841 cggaattgag atcgaagctc agctgcccgg gaagaagatg cgcagtctgt cggcctattc
     6901 aggaggcgag cgggtgctga tcgcgttatc gttgattttt gcgatgttgc gcgtgcaccc
     6961 cagcccattc tgtatctttg atgaggtgga ggcggcgctg gacgacgcga acacgaagaa
     7021 attcacgacg ctgctgcgag aactggcaga gcgcacgcag gtgctcatca tcacgcacaa
     7081 caaggggacc atggaagccg ccgacattct ctacggtgtc acaatggaag tccccggcgt
     7141 ttccaagatg atctcaatgc gcctggcgcg acaggagacc cccgagtcgg cgagtgttgt
     7201 ctaactgcgt cgtacttcgt gtggaacttt tacgaccgcc tgaaaaccgg gttaactcgc
     7261 acgcgagatg cgtttgcctc gcgcgttgac caggtcctcg cccaaccgct cagtcctgca
     7321 gtcttcgacg acctcgaatc cgcgctgatc caggccgacg tcggcgtgat cgccacgtcg
     7381 gtgatcatcg accgtctccg cgcgcggcgg gatgtccgcg acaccccctc ggtacgacag
     7441 gctttgcgcg cgatcattat cgagctcctc ggcaggagcg agcccctgcg catcgaaccg
     7501 cctccggccg ctgtgatcgt gctgggtgtc aacggggtgg ggaagacgac gacaatcggc
     7561 aagctcgcct accgtctctc ccgggatgga aggcgagttc tcctggctgc cgccgacacg
     7621 ttccgtgcgg ccgccattga gcaactcgcg gtctgggcgc agcgtgtggg cgctgacgtc
     7681 gtccgccacg cgcagggcgc tgatccggcg gccgtcgtct tcgacgcgat cgcggcggca
     7741 aaagcgcgcg ggatcgacgt cttgatcgtc gacaccgccg gccggctcca taccaaagtg
     7801 aacctgatgg aggagctcaa gaagatcaac cgggtcgtgg cgcgcgagct ggccggtcaa
     7861 ccggtcgagc gactactcgt gctggacgcc tcgatggggc aaaacgcgat cacgcaggcg
     7921 cggacgttca aggatgccgt cgatgtcacc ggcctcgtgc tcgcgaagct cgacgggacg
     7981 gcccggggcg gcgcggtgat cgcgatcgca cacgagcttc gtctccctgt gaaatttatc
     8041 ggtatcgggg aggcgctcga cgacctccag cccttcgagc cgcgcgaatt tgccgacgca
     8101 ctgctccccg gagcctgacc acgacatttg actctctcac cccttctgca gtagacttgg
     8161 gccagcctct gtaaagtaaa ttacctttac taacttgctt actcaacgac agctgactga
     8221 gcggttgcac attgtgcgtc tcgtcgaagt ctatggcggg ctgctgacag cccggcagct
     8281 ccggcagctc aggatgtact acctcgacga cctctcgctc ggcgaggtcg cggatcagtt
     8341 ggacgtgacc cggcaggccg tcttcgacag cttgaaacgt tcgctcgaca agttgaaccg
     8401 gctcgaaggc gagcttcacc tggtccgccg gccgcgcctg tagcaatgga gcactacttc
     8461 tccacccgcc cacaagttgt gtcccggccg cgggaggtgc atgcggtcct ccgcggcagg
     8521 aagtggacct ttctgaccga cagaggcgtc tttggccaca aaggcgtcga cgctgggacc
     8581 cggctgctca tcgataccat ggtcgtgcgt ccctcagatg acgttctgga tctcggctgc
     8641 gggtacgggc cggtcggcat cgtcgccgcg accctggcag tccaagggcg agtcgtgatg
     8701 atcgatgtca acgagcgcgc cgtcgcgctt gcggccgaga acgcacggcg tgcaagactc
     8761 gcgaacgttg aggtggtagc gggcgacgga ttagggcaac tggcgggaca aaagtttgac
     8821 gtcattgcga cgaacccgcc gatccgcgcc ggacgcgaag cactccgcaa cctctttcgc
     8881 gatgccggcg accatttacg tgatcagggg cgcttctatt ttgtggcccg caccgcgcag
     8941 ggtgcgaaga cgctcgccgc agtggtggat gcgatcattg gaaatgtaca ggagctggcg
     9001 aagggtgggg ggtaccgggt gtacgaggca acgaagaaag ctgaaaagct ggagagctaa
     9061 agagctgaat tctgatgtcg ttgctcttca gctttccagc tttccagctc ttcagctagg
     9121 agttgtgtaa tcgccaatgt tcgaaaacct tcaaggcagg ctacagaaga tcatcaccca
     9181 actcacgggc aggggcgtcc tctctgacgc ggatgtagac gcggcgctcc gtgaggtcag
     9241 gctggcgctg ctcgaggccg atgtcaactt caaggttgcg aagaccttca tcgatcgcgt
     9301 caaggcggcg gccatcggcc aacaggtgac ccaacacctc gccccgggcc agcaggtcgt
     9361 caagttggtg cacgaagagc tcgtcaggct cctcggtgga gcgtcgcatg aactgcgctt
     9421 tgcagatcgg tctccgacca tcgtcctcct cgtcggcgtc cacggggccg gcaagacgac
     9481 gacggcggcc aaactcgcgt cgcgactaaa gcggcgcgga cgccaacccc tgttggtggc
     9541 gaccgacctg cgccgccctg ccgccgtcac ccagctgcag ctcgtaggga cccaagtggg
     9601 cgtgcctgtc ttcagccagc ccggcgcgac agatccaatc ggggtcgcgc aggcatcact
     9661 cattgaagcg gagcgcagcg gccacgacgt ggtgatcgtc gacgctgcgg gccgccagca
     9721 tgccgacgac gagctgatgg ccgaactgcg ggcgatgcgg gatgtcctgc accctgataa
     9781 cgttgtgctc gtcctcgacg cgatgaccgg ccaggacgct gtcgcgatgg ctga
//
LOCUS       DBNGSB010000204         3046 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_1373753, whole genome shotgun sequence.
ACCESSION   DBNGSB010000204 DBNGSB010000000
VERSION     DBNGSB010000204.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_1373753"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="AC159B_06005"
     CDS             <1..519
                     /locus_tag="AC159B_06005"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3377332.1"
                     /translation="AFTFTVGSAEDIRLNPTLGGGGMMDVGCYCVNISRLLLGVPHTA
                     FATAQFEGVDVRLAGVLRFEHDRVAQFDCGLRAPFRQFCEVVGMSGTIYLERPFQPEE
                     RPATILIRRGEQERRVEIPGANQYVRMIDHFAECVQSNQAPRYPAEDAVANMRAIDAL
                     RESARSGQSVPI"
     gene            complement(633..1388)
                     /locus_tag="AC159B_06010"
     CDS             complement(633..1388)
                     /locus_tag="AC159B_06010"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR3377333.1"
                     /translation="MEPELAPNTAFAIVLLVIAFAAAVKGALGFGFPLIAVPLSANLI
                     GARTAVVLIAVSVVFGNLVILLRGGGRLQDARRFAGLLIGVTIGTVIGALAFDRLDPG
                     TLALIVGITSLLFAALGLLNVAPEISPRAQSYTGPSVGLASGIMGGTTGIFAPLIAAY
                     MHSLRLDKRGFVFWLTVSFLVGGLVQTLSYYRLGLYSGTILRYAAATFIPIIIGTQVG
                     FLIQDRLPSETFRRLVLVLVLASGLNLVLRQLL"
     gene            complement(1395..2510)
                     /locus_tag="AC159B_06015"
     CDS             complement(1395..2510)
                     /locus_tag="AC159B_06015"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HIR3377334.1"
                     /translation="MTLPIIDLQGSAFEGGVQHGRAAAGLIGRNLEIYYDRFQRETRL
                     PPQEVRDRAMRYLAVIDAWDPDYGQAMRGVSEGAKLPLADIAVLNARYEILYSQYSTI
                     NQGEEATLMVDGCTAFAVTPDASSDGHLYLGQNWDWFPQVEGVIVRTRRADGFALAAF
                     TEAGIVGGKIGMNSHGVGLVINGLLSNKDDWRRLRRPFHVRTWQILQAQTLDDAATVI
                     TRESRSCSANFLLGQAKGTARVMNIEAAPPAVCTITPRGGTLAHANHFEDPDALQVWQ
                     PLAEDRTTTYQRGARMHELLERGKAAGKLGKAGLQEILRDHGGHPDSVCRHPNPRLPE
                     EQRVESVVSVLEDLTARRFYVAGGTPCVTPFQEITLT"
     gene            complement(2546..>3046)
                     /locus_tag="AC159B_06020"
     CDS             complement(2546..>3046)
                     /locus_tag="AC159B_06020"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3377335.1"
                     /translation="YTQRAEELGVTFTTLADELKQRPDCLPAVYQLHCTLDIGAPRDD
                     PELPTPPTLEEFLNHSVRSPRALADAYILAKFREMYVGETVLKKSDADPSFLHQELTG
                     VLSDLRGLGIATALKVQGIEYARRHGYRQVQTFNSSKNEPMLAINTKFGFVRKPAWIE
                     FQKTLP"
BASE COUNT          580 a          949 c          950 g          567 t
ORIGIN      
        1 gccttcacgt tcacggtggg cagtgcagag gacatccgcc tgaatcccac cctcggcggt
       61 gggggcatga tggacgtggg ttgttactgc gtgaatatca gccgcctgtt gttgggcgtg
      121 cctcacaccg cgtttgccac ggctcagttc gaaggcgtcg acgtccgcct ggctggtgtg
      181 ctgcgctttg aacatgaccg ggtcgcgcag ttcgactgcg gcctgcgggc accgttccgc
      241 caattctgcg aggtggtggg catgtcaggc acgatctacc tggagcgtcc ttttcaacct
      301 gaggagcgcc ccgccacgat cctcatccgc cgcggggagc aggagcggcg cgtggagatt
      361 cccggagcaa atcagtacgt gcggatgatc gaccacttcg cagagtgcgt ccaatcgaac
      421 caagccccgc ggtaccccgc tgaagatgcc gtggccaaca tgcgcgcgat tgatgcgctg
      481 cgcgagtcgg ccagatctgg ccaatctgtt cccatttgac ctacatgagc taaaggaggg
      541 ggacagaccc ctcctgcagg aggggtctgt ccccctcctg ccgagcggat gttcgtgacc
      601 tgggatcgct gacatcatct agccccgctg gtttatagca gttgtcgcaa tacgaggttc
      661 agtccacttg caagcaccaa cacgagcacg aggcgacgga acgtctctga cgggagtcga
      721 tcctgaatca ggaatcccac ctgggtaccg ataatgatcg gaatgaaggt cgccgccgca
      781 tagcgcagta tcgttccgga atataggccc aggcggtagt aactcagcgt ctgcaccagc
      841 ccgcccacca gaaacgacac cgtgagccag aagacaaatc cccgtttgtc cagccgcaat
      901 gagtgcatat acgctgcaat gagcggggcg aagatgccgg tcgtgcctcc catgattcct
      961 gaggccagcc ccacgcttgg tccggtgtac gactgtgcgc gcggggaaat ctcgggggcg
     1021 acattgagca gtcccagcgc ggcgaacaga agcgatgtaa tccccacgat cagtgcgagc
     1081 gtgccgggat cgagacggtc gaacgccaac gcaccgatga cagtgccgat ggtcaccccg
     1141 atgagcagtc ctgcgaaccg gcgcgcgtcc tgcagacgcc cgccgccgcg cagcaggatg
     1201 acaagattgc caaacacgac cgagacagca atcaggacga ctgcggtgcg ggcgccgatc
     1261 aggttggcag agagaggaac ggcgatgagc ggaaagccga agccgagtgc gcccttgacc
     1321 gccgcggcga acgcaatgac caggaggacg atcgcaaacg cggtgttagg ggctaattcc
     1381 ggttccacac gagactacgt aagcgtgatc tcctgaaacg gcgtgacaca gggagttcct
     1441 cctgctacat agaaccgccg cgcggtcagg tcctcgagca ccgacacgac cgactcgacc
     1501 cgttgctcct ctggtagtcg aggatttgga tgccggcaga cggaatccgg gtggcctccg
     1561 tgatcgcgca agatttcctg gagacctgcc ttcccgagct taccggccgc ttttcccctc
     1621 tcgaggagtt cgtgcattcg cgcgccgcgc tgatacgtcg ttgtgcgatc ctcggcaagc
     1681 ggctgccaga cctggagtgc atcaggatcc tcgaagtggt tggcatgcgc gagtgtcccg
     1741 ccgcgaggcg tgatggtgca gacggcaggt ggcgcagcct cgatgttcat cacgcgcgcg
     1801 gtccccttgg cctgccccag gaggaagttc gccgaacacg agcggctctc gcgggtaatg
     1861 acggttgcgg cgtcgtcgag ggtctgcgcc tgcagaatct gccacgtccg cacgtgaaac
     1921 ggcctgcgca gccggcgcca gtcgtccttg ttgctgagca atccgttgat gacgagcccc
     1981 acgccgtgcg agttcatgcc gatcttgccg ccgacgatcc ccgcttcggt gaacgccgcc
     2041 agcgcgaaac catcggcccg tcgcgtcctc acgatgacgc cttccacctg cgggaaccag
     2101 tcccagttct gcccgaggta gaggtggccg tcactggacg catcgggtgt caccgcgaac
     2161 gccgtgcaac catcgaccat cagcgtcgct tcctcaccct ggttgatcgt tgaatactgg
     2221 ctgtagagga tctcataccg cgcgttgagg acggcaatgt ccgcgagcgg aagtttcgcg
     2281 ccctcgctca caccgcgcat cgcctgcccg tagtctggat cccaggcgtc gatcacggcc
     2341 aggtatcgca tcgcgcggtc acggacctcc tgcggtggga ggcgcgtttc acgctggaac
     2401 cggtcgtagt agatctctaa gttgcgaccg atcaggcccg cagcggcacg accgtgctgc
     2461 acgccgcctt caaaagcgga gccctgaaga tcgatgatgg gcagggtcat gggcgactcc
     2521 cttcgccgcg gccgcgagaa gtcatctagg gcagcgtctt ttggaactcg atccaggccg
     2581 gtttgcggac gaaaccgaac ttcgtgttga tggcgagcat cggctcgttt ttgctgctat
     2641 tgaacgtttg cacttgccgg tagccatgcc gccgcgcgta ttcgatcccc tggaccttca
     2701 atgctgtggc aatgccaagg ccccgcaggt cagataaaac cccggtcagc tcttggtgaa
     2761 ggaacgacgg atcggcatca cttttcttga ggaccgtttc gccgacatac atctcacgaa
     2821 acttcgccag aatgtacgcg tctgccagcg cgcgtgggct gcgcacggaa tggttcagga
     2881 actcttcaag agtcggcggc gtgggcagct ccggatcatc acgcggcgct ccgatatcta
     2941 gcgtgcagtg caactggtag acggctggga ggcaatccgg ccgttgcttc agttcgtccg
     3001 caagcgtcgt gaatgtcacg ccgagctcct ccgcccgttg cgtata
//
LOCUS       DBNGSB010000205        10002 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_914798, whole genome shotgun sequence.
ACCESSION   DBNGSB010000205 DBNGSB010000000
VERSION     DBNGSB010000205.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 10002)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10002)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10002
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_914798"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="AC159B_06025"
     CDS             complement(<1..626)
                     /locus_tag="AC159B_06025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3377336.1"
                     /translation="MARTKVLQRQADQGRADTDNMFRAYRVSRDDQLHAELTYRFMPL
                     VHQVARRYARSGIPTEDLHQIGYIGLMNAVGNFDPGRSVKFETYARHLIAGEIRHYLR
                     DQGTTVRRPRWLYEMDHRVSRAATELTQRKARTPVIQEIARELGISPDDVHEVLQARE
                     SLRFVSLDEEREGDDGAPMAQHDRIANTHLNGADLPVEERVLIGDAIER"
     regulatory      complement(677..766)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(942..1856)
                     /locus_tag="AC159B_06030"
     CDS             complement(942..1856)
                     /locus_tag="AC159B_06030"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HIR3377337.1"
                     /translation="MAQAGTTQPVRAGLGLLLAVLFALFLPGISDAASAFAEGRTAYL
                     RGDHGVAAVWLRSHLNRHPRDAAAWMWMGASYYQLGHHREAALSFTEAYRLRPSGDAA
                     LWLGASYVAAGDPGRAIAAFERAVKTQRHQTVLLAKQWLRSLEGQRMPVLGEDATPED
                     YAYVVSWYNPGLTSGQVDAIVRSVLHYSTAYRVDPRLVMSLIAIESGFRITAQSPAGA
                     YGLGQLMPATWQALRVHPGDPVANIYGTVRVLRGQMDRFGQDPSLALAAYNAGRGAVE
                     RHGGIPPYRETQWYVFNVLSLYRHLTGS"
     gene            complement(1916..2437)
                     /locus_tag="AC159B_06035"
     CDS             complement(1916..2437)
                     /locus_tag="AC159B_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HIR3377338.1"
                     /translation="MTLLRWLVPYRYARAIEDVDPRALRADGIRGVILDLDNTIVPWN
                     GVQPTPAVQRWIADLKDTGVQACIVSNNFTGRVRSVAERLSVPLVTGAIKPTPWAFRK
                     AMRVMGTTPRETALVGDQLFTDVLGGNLVGLKTILVDPLSPRDFPTTMLVRRIERLFR
                     RRVLKRVSPSPHA"
     gene            complement(2434..3417)
                     /gene="mltG"
                     /locus_tag="AC159B_06040"
     CDS             complement(2434..3417)
                     /gene="mltG"
                     /locus_tag="AC159B_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HIR3377339.1"
                     /translation="MRWSLFIVLAAVAAMVVVAVMAVLPVDPNGEPRLVFIPSGSSSA
                     DIGHRLERAGVIGRALDFVILVRLRGLTRSLQDGEYRLSPAMGLLDIVDRIARGAVVH
                     YPVTIPEGYTARQIVEELVQTRLGDRDEFLHLVRTGAKGYDLEFLGALTGTSLEGYLF
                     PDTYQIPRHLEERAALRLFLDRFGELVLPRWRESGRGRPLHEIVTVASMVEREARVPA
                     ERPLIAGVIYNRLARGWKLEVDATVLYALGQHKPIVTFADLKVDSPYNTYLHPGLPPG
                     PIANPGLAAIDAALNPTKTDYLYYVARADGSHVFSRTLGEHEAAVRRYRGR"
     gene            complement(3421..3693)
                     /locus_tag="AC159B_06045"
     CDS             complement(3421..3693)
                     /locus_tag="AC159B_06045"
                     /inference="COORDINATES: protein motif:HMM:NF018633.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HIR3377340.1"
                     /translation="MKDETDVFTLLDERGGSADFTLVDVVEVDQRRYAVLQPAAGGTP
                     REPDAAMVFRVENDTLVAIDDEAELERVVEALEATDSYDDITVLDG"
     gene            complement(3690..4106)
                     /gene="ruvX"
                     /locus_tag="AC159B_06050"
     CDS             complement(3690..4106)
                     /gene="ruvX"
                     /locus_tag="AC159B_06050"
                     /inference="COORDINATES: protein motif:HMM:NF015607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HIR3377341.1"
                     /translation="MRILGLDFGHVRIGAALSDPSAMIAQALEVIPHRGSRQVLGRIR
                     ELIAEYDVDRIVVGLPLRLDGREGEEAAAVRAFVAKLQRALSMPVEFADERLSTAEAE
                     KTMIEAGARREDRRGARDAVAAALILQTYLDRRGGS"
     gene            complement(4103..6748)
                     /gene="alaS"
                     /locus_tag="AC159B_06055"
     CDS             complement(4103..6748)
                     /gene="alaS"
                     /locus_tag="AC159B_06055"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HIR3377342.1"
                     /translation="MTWKQTADRFLAFFNGHGHTIVPSSSLVPANDPTLLFTNAGMVQ
                     FKDTLLGLERRPYSRAATLQKIMRVQGKHNDLENVGPSPWHHTFFFMLGNFSFGDYFK
                     GDAIAYAWELTTQQYGVDRERLIMTVLHGDDEAEAAWRKLGVPTSRVLRLGEKTNFWM
                     MGDVGPCGPTSELHYDWGPEYCTCGRPECSVALDNGCQRWLEFWNLVFMQFDQHSDGT
                     RVRLPTPGVDTGMGLERITSVLQGVHDDYRTDIFAPLMGRVRDLAGDQRVPGRVMADH
                     SRAMTFLMADGVVPGNEGRGYVLRMIMRRAMRFARSAGVTKPFLGDLAEVVIGEMGDA
                     FPELERQRSFIMEAARVEEARFAQTLTQGLSRLEEVIAAVRSRGERMVRGDDVFRLYD
                     TFGFPLEMTRDVAGEQGLAIDESGFAAAMDAQRDRARAAHPFAAPGAEDRRFADLANE
                     GVATEFIGYRKVRARAKVLAVFAGGHRVEEASDGSEVEVIIDRTPFYAEAGGQVGDTG
                     RLRASRVEVEVLDTQRPAGGVIAHRGRITRGRLRVGMPITAEVDVARRLDTMRNHTAT
                     HLLHRALQELLGEHARQAGSLVAPDRLRFDFVHLAALGDEQREAIERRVNERILDDLP
                     VVPKWMSYDEATKLGAMALFGEKYGDRVRVISIDEYSRELCGGTHVNRTGQIGMFKIT
                     SESGVAAGVRRIEGVTGAGVYALLRRQDSTLSTLADQLRVSPDEVPDRVRRVLERVKD
                     LERQVRSGTSGDGPKELLSRALEGAVDVSGLRFVAAEVPADDTETIRRLADQIRDRLD
                     ADHQPGVVVLASLATGQVVATRTRKTPVAVDVGRLVRSLTEAFGGSGGGRPEFAQGGL
                     KGGGRVADLIALARDRTFLERVVQP"
     gene            complement(7116..8402)
                     /locus_tag="AC159B_06060"
     CDS             complement(7116..8402)
                     /locus_tag="AC159B_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR3377343.1"
                     /translation="MPSNPDRLAAETLEKYGRFINPGLQRLYRFANVSTVEWEAQGAV
                     IRDAHGKEYLDCSGGPAIFNVGHRHPKVVAAVREQLDRMPMSVRALPRHLEADLAALL
                     AEITPGDLQYTFFCNSGAEANEGALKLARLATKRAEIIGTIGAFHGKTMGALSASGRD
                     TYKTPFEPLVPGFRHVAFGDLDAMASAVSARTAAVILEPIQGEGGVVIPPDEYLPGVR
                     RICDRHGALLIVDEVQTGLGRTGKMFACEHWGLAPDILTMAKALGGGVVPIGAFTGRP
                     HLWKALEQNPYLHSTTFGGNPLACAAGIATINVIREEHLVERAAESGTYFLERLRPVA
                     RRHSRFVKDVRGKGLLIGMEFTDPDLVLLVTAEALTRGVIVFYSLNKPDTFRIAPPLI
                     ITREQIDHAVAVLDESISAVEAQVEMGRETQPSERI"
     gene            complement(8392..8865)
                     /locus_tag="AC159B_06065"
     CDS             complement(8392..8865)
                     /locus_tag="AC159B_06065"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR3377344.1"
                     /translation="MVTQRRGRKPVKQARSAGGVVFRRDGERPHVLLLKHTSGKWMLP
                     KGTIESGETPEGVALREVREETGLSNVRVVADLGEERYYFFWRSEDTYYDKTVRYFLL
                     EFLGGEEPAPQAEEGFVACEWVALDEAIERIKYKETREVVRRAQTTLSGGDLDAK"
     gene            complement(8865..9317)
                     /locus_tag="AC159B_06070"
     CDS             complement(8865..9317)
                     /locus_tag="AC159B_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HIR3377345.1"
                     /translation="MALAQRLEQDLKSALRAGDAIRVSTIRLARAAIHNEEIERGRQL
                     SDDEIRVVLSKEAKRRREAIEMFEKGRRDDLVAKESLELAILTEYLPAGLAPEELRKI
                     VAEGIAQARATARKDTGKVMALVMPRIAGRADGQTVNQIVQEMLPEKA"
     gene            complement(9497..9670)
                     /gene="rpsU"
                     /locus_tag="AC159B_06075"
     CDS             complement(9497..9670)
                     /gene="rpsU"
                     /locus_tag="AC159B_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HIR3377346.1"
                     /translation="MTEVRVGKDESLDSALRRFKRQVKRSGILTDAKRHEHYESPSAK
                     RRRKLARRRRRGG"
     gene            complement(9828..9992)
                     /locus_tag="AC159B_06080"
     CDS             complement(9828..9992)
                     /locus_tag="AC159B_06080"
                     /inference="COORDINATES: protein motif:HMM:NF013401.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HIR3377347.1"
                     /translation="MGALTLAAVQLARAEGLDHRGFRLVINVGPEGGQTVPHLHMHLL
                     GGRQMKWPPG"
BASE COUNT         1842 a         3295 c         2986 g         1879 t
ORIGIN      
        1 cgttcgatcg catcgccgat taatacacgc tcttccaccg gcaggtcggc gccgttcagg
       61 tgtgtgttgg ctattcgatc gtgttgggcc atgggcgcgc cgtcgtcgcc ctcgcgctcc
      121 tcgtccagcg agacgaacct caacgactct cgcgcctgca agacttcatg cacgtcgtcg
      181 ggactgatgc ccaactcacg cgcgatctcc tggatgacgg gtgtccgggc tttgcgttgc
      241 gtgagctcgg tcgcggcgcg ggagacgcga tggtccatct cgtagagcca tcgcggccgc
      301 cgcacggtcg tcccctggtc ccgaaggtaa tggcgaatct cgcccgcaat gaggtggcgc
      361 gcgtatgtct caaatttcac gctgcggcct ggatcgaagt tgcccaccgc gttcatgaga
      421 ccgatgtagc caatctggtg gagatcttcg gtgggaattc ctgaacgggc gtaccgccgg
      481 gcgacctggt gcaccagcgg catgaaccgg taggtcagtt cggcatgcag ctggtcgtcg
      541 cgcgacacgc ggtaggcacg gaacatgttg tccgtgtccg cgcgtccctg gtcggcctga
      601 cgctgcagca ctttcgtacg cgccataata aaacatcctc cctcgaggcc tgcgaaggag
      661 gattaaaaac gcctcgttcg gcggcccggc tgacccccca agtagtcggt cggagggtcc
      721 cgtggctttg cgcccccgtc ttacgacggg tttgcccttt cgcgagacct cgtatgaaat
      781 ttgtggaact tacttcttag tttacacctt tcggcgagtc ccagccaaat ccttcctcca
      841 accttctcgg tcgaggaagc gaatcagcgg aaggtactag caattttgat gccatttcct
      901 tgaattgtct atgccccgaa ttggagtatt ctagcaactt gctagctgcc tgtcaggtgc
      961 cgatacagcg aaagcacatt gaagacatac cactgtgttt cccggtacgg cggaatgccg
     1021 ccatgacgtt caaccgcacc acgcccggca ttgtaggcag ccagggcaag cgacgggtcc
     1081 tggccgaagc gatccatctg accacgaaga acacgcaccg tcccgtaaat attggcgacg
     1141 ggatcccccg gatgcaccct cagggcctgc caggtcgccg gcatgagctg accaagaccg
     1201 taggcgccgg ccggggactg cgcggtgatg cggaagccgg attcaatggc gatgagagac
     1261 atcaccaacc gcggatcaac gcggtaggcg gtggaatagt gcagcacgct gcgcacgatc
     1321 gcgtcgactt gcccggacgt gagcccggga ttataccagg agacgacata agcgtagtct
     1381 tccggtgtag cgtcctctcc gagcaccggc atacgctggc cttccaggct gcgcagccat
     1441 tgtttggcga gtagcacagt ctgatgacgc tgcgtcttta cggctcgttc aaacgccgca
     1501 atcgcgcgtc cgggatctcc ggctgccaca tacgaggcgc ccaaccacag ggccgcgtcg
     1561 cccgagggtc tcagccggta cgcttcagta aacgagagcg ctgcctcccg atggtgaccg
     1621 agttgatagt acgaggcgcc catccacatc caggcggcgg cgtcgcgcgg gtgtctgttc
     1681 aggtgggacc ggagccagac ggcagccacc ccatggtccc cgcgcagata cgccgtgcgc
     1741 ccctcggcaa aggctgatgc agcgtcgctg atgcccggga ggaacaatgc aaaaaggact
     1801 gccaggagca gtcctagccc ggcgcggact ggttgcgtgg tgcccgcctg agccatatcg
     1861 cctctctgca ctattgggga ggtgcatcag ttgatatgca cggagaggac tgctgttagg
     1921 cgtgtggcga gggggagacg cgcttgagca cccggcggcg aaagagccgc tcaatccggc
     1981 ggacgagcat cgtggtgggg aagtcgcgtg gggaaagggg gtcgaccagg atcgttttca
     2041 aaccgaccag gttaccgccc agcacatcgg tgaacagctg atcgccgacc agcgccgtct
     2101 ctcggggtgt cgtccccatg acccgcatcg ccttgcggaa cgcccagggg gtgggcttga
     2161 tcgcaccggt cacgaggggg acgctgaggc gctcggccac ggagcggacc cttcccgtga
     2221 agttgtttga cacgatacag gcctgaacac cagtatcttt gagatcggcg atccagcgtt
     2281 gaaccgcggg cgtcggctgc accccgttcc acggcacgat cgtgttgtcg agatcgagaa
     2341 tcacgccgcg gatgccgtcg gcgcggagcg cccggggatc cacatcttcg atcgcgcgag
     2401 cgtaccggta gggcacgagc cagcgtagta gcgtcaccgt ccgcgatacc ggcgtacggc
     2461 cgcctcatgc tcgcccaacg tccggctgaa cacgtgcgag ccgtcggccc tggcgacgta
     2521 atagaggtaa tcggttttcg ttggattgag cgcagcgtcg atcgcggcca gcccagggtt
     2581 tgcgatgggt ccgggtggta gccccggatg gagatatgta ttgtatggtg agtcgacctt
     2641 cagatcggcg aaggtgacga tcggcttgtg ctggcccagc gcgtagagga ccgtggcgtc
     2701 cacctcaagt ttccatccgc gtgccaaccg attgtagatc acgccggcga tcaatgggcg
     2761 ttcggccggc actcgcgctt cgcgctccac catggacgcg accgtcacga tctcgtgcag
     2821 cggccgtcct ctgcctgact cgcgccaacg agggagaacg agttcgccga atcgatccaa
     2881 gaacagccgc agcgcggcgc gctcctccaa gtgtctcgga atctgatagg tgtccgggaa
     2941 aaggtatcct tcgagcgatg tccccgtaag cgcgccgaga aattcgaggt cgtacccctt
     3001 cgccccggta cgaaccaggt ggaggaactc atcccggtcg ccgagtctgg tctgcacgag
     3061 ttcttcgacg atctgccggg ctgtgtagcc ctctgggatc gtcactggat aatgcacgac
     3121 cgcgccacgg gcgatgcggt ccacgatgtc gagcaacccc atggcggggg agaggcggta
     3181 ctcaccgtcc tggagcgatc gagtcagacc gcgcaaccgc accaggatga caaaatcgag
     3241 ggcgcggccg ataacgccag cgcgctcgag acggtggccg atgtcagccg acgatgaccc
     3301 ggagggaatg aagacaaggc gaggctcgcc gttcggatca acagggagga ccgccatgac
     3361 ggccaccaca accattgccg cgaccgccgc caggacgatg aacaatgacc agcgcatgca
     3421 tcagccgtcg agcacagtga tgtcgtcgta tgagtccgtc gcttcgagcg cttcgacaac
     3481 acgctcaagc tccgcttcgt catcgatcgc gaccaacgtg tcattctcca cgcggaatac
     3541 cattgccgca tcaggttcgc gcggggtccc ccccgccgcc ggctgaagga ccgcgtaacg
     3601 ccgctggtct acttcgacga catcgacgag agtaaaatcc gcactccccc ctctctcgtc
     3661 gagtagtgta aagacatcag tttcgtcttt cacgatccac ctcgtcgatc cagatacgtt
     3721 tgcaggatta gcgccgccgc gacggcgtcc cgtgcgcccc gccggtcttc gcggcgcgcg
     3781 cccgcttcga tcatcgtttt ctccgcttcc gcggtagaga gccgttcatc cgcaaactca
     3841 acaggcatcg acagcgcgcg ttgaagcttg gcgacgaacg cccggacggc cgcggcttcc
     3901 tccccttcac gtccatccag ccgcaacggc aggcccacaa cgattcgatc gacgtcgtac
     3961 tctgcgatca gctcccgaat gcggcccagc acctgccggg atccacggtg cgggatgacc
     4021 tcgagcgcct gggcgatcat cgcactcggg tctgaaagag cggcaccgat gcgaacgtgg
     4081 ccaaaatcca gtcccaggat tctcacggct gcacgacccg ctccaggaac gttcgatcgc
     4141 gcgccagagc aatcaggtcc gccacccgcc cgccgccttt cagacctccc tgcgcaaact
     4201 ccggccgccc gccgccgcta cctccgaatg cttcagtcag cgaccggacg agccggccga
     4261 cgtcaacggc gaccggcgtc tttcgggttc gtgtggccac gacctggccg gtcgcgaggg
     4321 acgccagtac gaccaccccc ggctggtggt ccgcgtcgag ccggtcgcga atttgatcgg
     4381 caagccttct gatcgtttca gtgtcgtcgg ccgggacctc ggcggccaca aaccgcaatc
     4441 cggaaacgtc caccgcccct tcgagcgcac gcgacaagag ctctttcgga ccgtcaccgc
     4501 tcgtcccgct tcgaacctgc cgttcgaggt ccttcactcg ctcgagcact ctccggacac
     4561 ggtcgggcac ttcgtccggc gagacgcgca gctgatccgc gagcgtgctc aaggtagaat
     4621 cttgcctacg aagcagcgcg tagactccag ctccggtcac cccttcgatt cttcggaccc
     4681 cggcggctac cccgctttcc gaagtgatct tgaacatgcc gatctggcca gtacgattca
     4741 catgcgtgcc gccacacagc tcgcgactgt actcatcgat actgatgacg cgcactcggt
     4801 caccatactt ttcgccaaac agcgccatag cgccaagctt cgtggcctca tcatagctca
     4861 tccactttgg gacgaccggc aggtcgtcaa ggatcctctc gttcacgcgg cgctcgatcg
     4921 cctcgcgctg ttcgtcgccg agcgccgcga ggtgaacgaa gtcaaatcgc aggcggtcgg
     4981 gcgcgacgag cgaaccggcc tgtctcgcat gctcgcccag cagttcctgg agcgccctgt
     5041 gcagcaggtg ggtggcggta tgattgcgca tcgtgtccag tcgacgcgcc acatcgacct
     5101 cggccgtgat cggcatgcca acgcgcaggc ggccacgcgt aatgcggccg cggtgcgcga
     5161 tgaccccgcc cgccggccgt tgcgtatcca ggacctccac ctcgacgcgc gacgcgcgaa
     5221 gccggcccgt gtcgccgacc tgcccgcctg cttccgcata gaagggggtc cggtcgatga
     5281 tcacctcgac ctcgctacca tccgaggctt cctcgacacg atggccgccg gcaaacacgg
     5341 ccagcacttt tgctctcgcg cgcaccttac gatacccgat gaattcggtc gcgacgcctt
     5401 cgtttgcaag atccgcgaac cgccggtctt cggccccggg agccgcgaac gggtgcgcgg
     5461 cgcgggcgcg gtcgcgctgc gcgtccatgg cggccgcaaa gcctgattca tcgatcgcca
     5521 gcccctgctc gccggcgacg tctcgtgtca tctcgagcgg aaatccgaag gtgtcgtaca
     5581 ggcggaagac gtcgtcgccg cggaccatcc tttcgccgcg gctgcgcacc gctgcaatga
     5641 cttcctccaa gcgactgagt ccctgggtca gcgtttgggc gaagcgggct tcttcgaccc
     5701 gcgcggcttc cataatgaag gaccgctggc gttccaactc aggaaacgcg tcgcccatct
     5761 ctccgatcac cacctcggcc aagtcgccaa gaaacggctt ggtcacccct gcactgcgcg
     5821 cgaaacgcat cgcccgacgc atgatcatcc ggaggacata tccgcgccct tcgttgcccg
     5881 gcacgacccc gtcggccatg aggaatgtca tcgcccgaga gtggtcggcc atcacgcgtc
     5941 caggaacgcg ctgatcgccg gcgagatccc tcacccgccc catgagagga gcaaagatgt
     6001 cggtgcggta gtcatcgtgg acgccctgca gaacagaagt gatacgctcc aaccccatgc
     6061 ccgtatcgac gccgggcgtc ggcagccgca cgcgggtgcc gtcggagtgc tggtcaaact
     6121 gcatgaaaac aaggttccag aactccaacc acctctggca accgttgtcc agcgccacgc
     6181 tgcattcagg ccgtccgcag gtacagtact ctggtcccca gtcgtagtga agctcggatg
     6241 tcggcccgca cgggccgaca tcgcccatca tccagaagtt tgtcttctcc ccgaggcgaa
     6301 ggacccgcga cgtcggcacg ccgagtttgc gccaggccgc ctccgcctca tcatcaccat
     6361 gcaacaccgt catgatgaga cgctctcggt ccacgccgta ctgctgagtg gtaagctccc
     6421 aagcgtatgc gatcgcatcg cctttgaagt agtcaccaaa gctaaagttt ccaagcatga
     6481 agaagaacgt gtggtgccag ggcgagggcc cgacgttctc aaggtcgtta tgcttgccct
     6541 gcacccgcat gatcttctgc agggtcgccg cccgcgaata cggccgccgc tctaaaccga
     6601 gcagcgtatc tttgaactgc accatgccgg cgttcgtgaa caagagcgtc gggtcgttgg
     6661 caggcacaag cgaggagctg ggcacgatcg tatgcccgtg cccattgaag aacgctaaga
     6721 accgatcagc agtctgcttc catgtcatca taaaaacaaa accccctcgt cgcagggacg
     6781 agaggtatcc tcccgtggta ccacccgaat tctggccgtg gccgaaacgc gacacgacca
     6841 gcgctcaatc ccggtatcgg cgggtccgga cgacttccgc attcgcgttc gccgtcggct
     6901 cgggggtggc gagccccgtc atcgcttcaa cttcagttgt agtgtatgag cggtggcata
     6961 gtatgtcaaa cagaagatag ccttaactcg ctccggagtg ggttccaaca ggtaccctca
     7021 gcctattgat cccgcgcaac tcgctccgga gcgagttgcg gcaatgccgg gggccgcagg
     7081 gcaattctta ctcgatcgaa ccgggagggc gtggcctaga ttcgctctga gggctgcgtt
     7141 tctctgccca tttcaacctg cgcctcaacc gccgagatcg actcgtcgag gacggccacg
     7201 gcgtggtcga tttgttctcg cgtgatgatg agtggcggag cgattcgaaa tgtgtctggt
     7261 ttgttcaaac tgtagaacac gatcacgccc cgcgtcagtg cctcagccgt caccagcaat
     7321 acgaggtccg gatccgtgaa ctccatgccg atgagcaggc cttttccgcg aacgtctttt
     7381 acgaatcgcg aatgtcgccg cgcaacgggg cgcagccgct ccaagaaata tgttcccgat
     7441 tcagcagccc gctcgacgag atgttcctcg cgaatcacgt tgatcgtggc aatgccggcg
     7501 gcgcacgcca gcgggttccc accgaaggtc gtggagtgca gatacgggtt ttgctccagc
     7561 gccttccata agtgcggccg cccggtgaac gcaccgatcg gaacgacgcc tccaccgagt
     7621 gccttcgcca tcgtcaagat gtcgggcgcc aggccccagt gctcgcaggc gaacatcttg
     7681 ccggtgcgcc caagacccgt ttgcacctcg tcaacaatga gtaacgctcc gtggcgatca
     7741 cagatgcggc gcaccccagg aaggtactcg tcgggcggaa tgaccacccc gccctcgccc
     7801 tggatcggct ccaggataac ggctgcggtt cgcgcgctga ccgccgaggc catcgcgtcg
     7861 aggtcaccga aggccacgtg ccggaatccc ggcaccagtg gctcaaacgg agtcttgtag
     7921 gtatcacggc ctgacgcgga cagcgcgccc atcgtcttgc catggaacgc cccgatcgta
     7981 ccgatgatct ctgcgcgctt cgtggcaagc cgcgcgagct tcaacgcgcc ctcattggcc
     8041 tcggcgccac tgttgcagaa gaaggtgtac tgcagatccc ccggcgtgat ctctgcaaga
     8101 agcgcggcga ggtctgcctc caggtggcgc ggcagggcgc gcacggacat cggcatgcgg
     8161 tcgagttgct ccctcacggc cgccaccacc ttggggtgcc gatgcccgac gttgaagatc
     8221 gccggcccgc cggagcagtc gagatactct ttgccgtggg cgtcgcggat cacggcgccc
     8281 tgagcttccc actccacggt ggagacgttg gcgaatcggt agaggcgttg cagccccgga
     8341 ttgatgaacc gaccgtactt ctcgagcgtc tcggccgcga ggcgatccgg attacttggc
     8401 atcgaggtct ccgccagaga gcgtcgtctg cgcccttcgg acgacttccc tcgtttcctt
     8461 gtatttgatg cgttcgatcg cttcgtcgag cgccacccac tcgcacgcga cgaatccttc
     8521 ctccgcttgg ggcgcaggct cctctccgcc cagaaactct aagaggaagt accggactgt
     8581 cttgtcatag tacgtgtctt cagagcgcca gaaaaagtag taccgctcct cgccgagatc
     8641 cgccaccact ctgacattcg agaggccggt ctcctcacgc acctcacgca gcgcgacgcc
     8701 ctcgggcgtc tcacccgatt caatcgtccc tttgggaagc atccactttc cgcttgtgtg
     8761 cttcaacagc aggacgtgcg ggcgttcgcc gtctcttcgg aagacgacgc ctccggcgct
     8821 tcgcgcctgc ttcaccggtt tccgaccgcg gcgctgagtg accatcacgc tttctcaggt
     8881 aacatctcct gtacgatctg gttcaccgtt tgaccgtctg cgcggccagc tatgcgcggc
     8941 atgacgaggg ccatgacctt gcccgtgtct ttgcgcgccg tcgctcgagc ctgagcgatt
     9001 ccctccgcga cgatcttccg tagttcctcc ggcgccagac ccgccggcag gtattccgta
     9061 aggatggcaa gttcaagcga ctctttcgcc acgaggtcat cgcgtctgcc cttctcgaac
     9121 atttcgattg cctcgcgcct tcgcttcgct tctttgctca agacgacgcg gatctcatcg
     9181 tccgagagct gccgtccccg ctcaatctcc tcgttgtgaa ttgctgcacg cgccagccgg
     9241 atcgtcgata cgcgaatggc gtcgccggcc cgcaaggcag acttcagatc ctgttcaagt
     9301 cgttgagcta atgccatacg ttacaaacga gccgggtggt aacaaagtga tgcaaatgcg
     9361 caagcgcgga agcgcgcaag caatggattg cgcgtttgcc cgattgcgcg attgcgcaga
     9421 atgcagaaag ggggactggt gtcccccttt gtgagtttcg atccggcgat cctctcatgc
     9481 tcgctggcgc agtgggttac cctccccgcc ggcggcgacg ggcaagcttt ctcctgcgct
     9541 ttgcgctggg tgattcgtag tgctcgtgcc gtttcgcatc cgtcaaaatt cccgaccgct
     9601 tgacctgacg cttgaatcgt cgcagagcac tgtccaatga ctcgtccttg ccgaccctca
     9661 cttcagtcat ctcaacaccc cccgcctgcc tgagcgtggt gcaaacttga aacattataa
     9721 cccaggaaac aggtaacagg aaacagggag cagataacag accgtctggc cggtttctga
     9781 gtcgtacctg tttcctgcga cctgcttcct gcttccgcag ttccgggtca gccgggcggc
     9841 cacttcatct ggcgtccgcc aagcaggtgc atgtggagat gcggtaccgt ctgcccgccc
     9901 tcggggccga cgttgatgac cagacggaaa ccgcggtggt cgagtccttc tgctctggcg
     9961 agttgaactg ccgcgagggt gagcgctccg atgagcggcg ca
//
LOCUS       DBNGSB010000206         4414 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3981539, whole genome shotgun sequence.
ACCESSION   DBNGSB010000206 DBNGSB010000000
VERSION     DBNGSB010000206.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 4414)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4414)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4414
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3981539"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /locus_tag="AC159B_06085"
     CDS             <1..1134
                     /locus_tag="AC159B_06085"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR3377348.1"
                     /translation="RGPVGYRGASMMLADLDTPILLVDLPRMERNIHTMAEFARDAGV
                     ELRPHVKTHKIPALAHLQLAAGARGVTVAKVGEAEVMADAGITDILVCYPILGEEKLE
                     RLAHLARHARIAVAVDSFEAARQLSEAAGRYGVHFELLLEVDSGLNRCGLPPGDPVVD
                     LAKQVLRLDGLEISGVCTHAGHAIRAGTQNERDAIARHEGDSVVFTRDRLKAAGIRIR
                     EVSVGSSATVRVSGRVPGVTEIRPGTYIFNDYMQTTAGACSEDDCALTVLSTVVSRPS
                     PDRVILDAGSKCLSSDFHRMTERMSGYGYIKGSRGTLVSRLSEEHGVVQLSDGDPPLA
                     IGDRVEIIPNHACAALNLWDDLHGVRNGEVETVWPVLARGKVR"
     gene            1131..2189
                     /locus_tag="AC159B_06090"
     CDS             1131..2189
                     /locus_tag="AC159B_06090"
                     /inference="COORDINATES: protein motif:HMM:NF013567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3377349.1"
                     /translation="MSKRIYRAGIIGCGRKAATLDDEERCRTNYSIAPDAHAAAYKQI
                     PEVALAAAADPNASKRDLMRERWGVPAVYKDYREMLTREDLDLVSVTTRAPLHAEVTI
                     AAAKAGVRGILCEKAMATSLREADAMIEACKRARTKLLINHTRRWHPTYARATETVRA
                     GAIGALRCIVGVCPGPLIHNGTHLFDLMRLFGGDVKWVCGEVQPGGAQDAPGRAMLAF
                     QNGAAGFVDLDSSVGLALELQCTTGRILIDSSEDGFAIWEYQDPPRSSGLAWYQGNAC
                     KPRSIRHVDSGGGVGTLVAAIAELITCVEEDHEGRSSGQDGRAALELALAVYQSHAHG
                     GMRVTLPLQDRALTVPSR"
     gene            2199..3359
                     /locus_tag="AC159B_06095"
     CDS             2199..3359
                     /locus_tag="AC159B_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HIR3377350.1"
                     /translation="MGTTLLRNCKALHLEGIPSGSTIDIGIGADGTVVHAKGTPRFDK
                     VIDCAGGWLSPAWIDLHVHCYYGGTWLSVRPEHVGPMTGVGLAVDCGSAGEANFTGLR
                     ELIIDAAPFPILAYLNVSSIGLVAANRVSELISDAVLHPVRAADCVEKHRDVIRGIKV
                     RASDQVVKQWGIAPVRAARQLARAVGLPLVVHIGEAPPALDEILDLMEPGDMITHCFT
                     GRITTSISRHEPYFRRVQSLASEGLLLDVGHGQGSFNYATARWAVERGLLPTSISTDL
                     HIGCIWGPAWDLATVMSKMLAVGMSVEDVVARVTSNPAAFLHLKGWGRATPGTPARFT
                     VFELSEGEERLPDSDGNMEVVGRFFEPRYTAMGPVLTPAARNTTRDRRSISI"
     gene            3388..4344
                     /locus_tag="AC159B_06100"
     CDS             3388..4344
                     /locus_tag="AC159B_06100"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HIR3377351.1"
                     /translation="MQIPSLADVLRARLQIRPYLRHTPLFSYAALSRLVGTEVWIKHE
                     NHQPVGAFKVRGGVNLISQLSADEQRRGVIAASTGNHGQSIAYAAKLFGIRAIICVPE
                     GANPVKVESMQDLGAEIIVHGRDYDDAREYCERLAADEGYRYIHSGNEPLLIAGVATA
                     TLEILEEQPDVDLIIVPIGGGSGAAGACIVADSIRPQTQVIGVQSEAAPAGYRSWQSR
                     QLVEDRMGTYAEGLATRTAFELPQRILWEHLDDFVLVSDDEIRQAQRTIIEKTRNLVE
                     AAGASPLAAALKIKEQLTGKRVAMICSGGNVSPEQLRQLFVT"
BASE COUNT          839 a         1324 c         1452 g          799 t
ORIGIN      
        1 cgcggcccgg tcgggtaccg aggtgcctcg atgatgctcg cggacctgga caccccgatc
       61 ctgctggtcg atttgccccg catggaacgg aacatccaca cgatggcgga gttcgcgcga
      121 gatgccggcg tcgagctccg cccccatgtc aagactcaca agatccctgc cctcgcacat
      181 ctgcagctcg cggccggcgc ccgcggcgtg acggtcgcga aagtgggcga ggccgaggtg
      241 atggccgacg caggcatcac ggacattctt gtatgctatc cgatccttgg cgaggaaaag
      301 ctcgagcgac tggcccacct tgcccgccac gcacgcatcg ccgtggccgt tgattccttc
      361 gaagccgcgc gccagctttc tgaagctgcg ggaaggtacg gggtgcactt cgagcttttg
      421 ctcgaagtgg actcgggcct caaccggtgc gggctgccgc ccggggatcc ggtggtcgat
      481 ctcgctaaac aggtcctgcg gctcgacggc ctcgagatct ccggcgtctg cacgcacgca
      541 ggccacgcca tcagagccgg aacgcaaaac gagcgcgacg ccattgcccg ccacgagggg
      601 gatagcgtcg tcttcacgcg cgaccgcctg aaagcagccg ggattcggat tcgcgaggtg
      661 agtgtcgggt cgtcggcgac ggtgcgcgtg tcgggccgcg tgccgggcgt gactgagatc
      721 cgcccgggga catacatctt caacgactac atgcagacaa cggcgggcgc ctgcagcgag
      781 gacgattgcg cgctgaccgt gctcagtaca gtcgtcagcc gtccttcacc cgatcgagtc
      841 atcctggatg cgggaagtaa gtgcctctcc agtgatttcc atcgcatgac cgagcgcatg
      901 tcaggctatg gatacattaa aggaagtcgc ggcacgctcg tgtcgaggtt gagtgaggag
      961 catggcgtag tccagttgag cgatggcgat cctccgctgg cgatcgggga tcgcgtggag
     1021 atcattccga accacgcctg cgcggcgctg aacctgtggg atgacctcca tggagtgcgc
     1081 aacggcgagg tagaaaccgt ttggccggtg ctggcacgag ggaaggtccg gtgagcaaac
     1141 gtatctaccg cgccggcatc atcggctgcg gccgtaaggc ggcgacgctg gacgacgaag
     1201 aacgctgccg gaccaactac agcatcgcgc cggacgctca tgctgccgcg tacaagcaga
     1261 tcccggaggt ggcgctcgcc gcagctgctg accccaatgc atccaagcgt gatctgatgc
     1321 gcgaacggtg gggagttccc gccgtgtaca aggactacag ggagatgctg acccgcgaag
     1381 atctcgatct ggtcagcgtc accacgcgcg cgccgctgca tgccgaggtc accatcgcgg
     1441 ccgcaaaggc aggcgtgcgc ggcatcctgt gcgagaaggc catggcgacc agcctgcggg
     1501 aggccgacgc catgatcgaa gcgtgcaagc gggcgagaac aaaacttctc atcaaccaca
     1561 cccgacggtg gcatccaacg tacgcgcggg ccaccgagac cgtgcgcgcc ggcgccattg
     1621 gagccctccg ctgcattgta ggagtgtgcc cgggccccct gatccacaac ggcacgcacc
     1681 tcttcgattt gatgaggtta ttcggtggag acgtgaaatg ggtgtgcggc gaagtccagc
     1741 cgggtggagc tcaggatgca ccgggccggg ccatgctggc ttttcagaac ggcgcggccg
     1801 gatttgtcga tctggacagc agcgtaggct tggccctcga attacaatgc acaacgggcc
     1861 gcatcctcat cgattcctca gaagatgggt tcgcgatttg ggagtaccag gatccaccgc
     1921 gttcatccgg gctggcctgg taccagggca acgcctgcaa gccgcggtcc attcggcatg
     1981 tcgacagcgg cgggggcgtg gggactcttg ttgcagcgat cgcggagctc atcacgtgcg
     2041 tcgaggagga tcatgaaggc cgctcgagcg gtcaggacgg gcgggcggcg ctggaactcg
     2101 ccctcgccgt ttaccagtcg cacgcgcatg gaggaatgcg tgtgacgctc cccctgcagg
     2161 accgagcgct gacggtgccg tcgcgctaag agatggacgt gggaacaact ctgctgcgga
     2221 actgcaaggc attgcacctg gagggaatcc cttccggctc gacgatcgat atcgggatcg
     2281 gcgccgatgg cactgtggta cacgccaagg gcactccgcg ttttgacaag gtcatcgact
     2341 gcgccggtgg ctggctgtcg ccggcatgga tcgaccttca cgtccactgc tactatggtg
     2401 gcacgtggct gtcggtgcgg cccgagcacg ttggacccat gacgggcgtt gggctcgcgg
     2461 tcgactgcgg cagcgcgggc gaagccaatt tcaccgggct gcgcgagttg atcatcgatg
     2521 ctgctccgtt cccgatcctc gcttacctga atgtcagctc tatcggtctc gtggcagcca
     2581 atcgcgtcag cgaactgatc agtgacgccg tgttgcaccc ggttcgtgcg gcggattgtg
     2641 tggagaagca ccgggatgtg atccgtggca tcaaagtgcg ggcgagcgat caggtcgtaa
     2701 agcagtgggg gatcgcgccg gtgcgggcgg ccaggcagct ggcccgcgcg gtcggtctgc
     2761 cccttgtggt gcacatcggt gaggcgccgc cggcgctcga cgagatcctc gacctcatgg
     2821 aacctggaga tatgattact cactgcttca ccggccggat cacgacgtcg atctcgcgac
     2881 atgaacccta tttccgtcgt gtgcagtctc tggcaagtga gggcctgctc ttagatgtcg
     2941 gccacggaca gggcagcttc aactacgcga ccgcccggtg ggcggtcgag cggggactct
     3001 tgccgaccag catcagcacc gaccttcaca ttggctgcat ctggggaccg gcatgggacc
     3061 ttgccacggt gatgtctaag atgctcgcgg tcggaatgtc ggtcgaagac gtcgtagctc
     3121 gagtgacatc gaatccagca gcgtttcttc atcttaaagg gtgggggcgg gcgacacctg
     3181 ggacgccggc tcgcttcacc gtattcgaac tgagcgaggg ggaggaacga ctccctgatt
     3241 ccgatggcaa tatggaagtc gtaggccgct tcttcgaacc gcgctataca gcgatgggcc
     3301 cggtcctcac tcccgccgcc cgcaacacta cgcgcgacag gcggagcatt tcgatctaat
     3361 cttaacttca tggaccacgg agaggagatg caaattccat cactggcaga cgtgctgagg
     3421 gcgcggctgc agatcaggcc atatctgcgg cacacaccgc tattttccta tgccgcgctg
     3481 agccggctgg tagggactga ggtgtggatc aagcacgaaa atcaccagcc ggtaggggcg
     3541 tttaaggtgc gcggcggtgt gaatttgatt tcacagttga gcgctgatga gcagcggcgc
     3601 ggcgtgatcg ccgcctccac cggcaatcac ggccagtcga tcgcgtatgc ggcaaagctc
     3661 ttcggcatac gcgcaatcat ctgtgtcccg gagggggcca atcccgtcaa ggtcgagtcg
     3721 atgcaggacc tgggcgcaga gatcatcgtt cacggtcgcg actacgacga cgcgcgggaa
     3781 tactgtgagc ggctggccgc cgacgaaggg taccggtata tccactccgg caacgagccg
     3841 ctgctgattg cgggtgttgc tactgcaacg ctcgaaattc tcgaagaaca gccggacgtc
     3901 gatctcatca tcgtgccgat cggcggaggc agcggcgcgg caggcgcttg catcgtcgcc
     3961 gactcgatac ggccacagac tcaggtcatc ggcgtacagt ctgaggctgc ccctgccggc
     4021 tatcgctcct ggcagtcccg acaactggtc gaagaccgaa tgggaacgta cgcggagggc
     4081 ctcgctacgc gaacggcatt cgagctccca cagcggatcc tgtgggagca cctagacgat
     4141 ttcgtcttgg tcagcgacga tgagatccgc caagctcaac ggacgattat tgaaaaaacg
     4201 cgaaatctgg tggaggcagc cggcgcctct ccgctcgccg ccgcgctcaa gattaaggaa
     4261 cagttgacag gcaaacgcgt cgcgatgatc tgcagcggtg gaaatgtcag tccagagcaa
     4321 ctgcgccagc ttttcgtgac ctgaatgtta atgatttggt gaagacggcg ggatttgctg
     4381 cgatcgatcg cctacggatc tttcacttca atgg
//
LOCUS       DBNGSB010000207         2523 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_3983994, whole genome shotgun sequence.
ACCESSION   DBNGSB010000207 DBNGSB010000000
VERSION     DBNGSB010000207.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_3983994"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="AC159B_06105"
     CDS             complement(<1..653)
                     /locus_tag="AC159B_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR3377352.1"
                     /translation="MAISELQPRFQSPLASLIEQFVQTKQACGYRFGEGIRLLRCFDR
                     YLIAEGLLQCELPRALVRQWLAKQSHESARTQQCRISLVRQFAGYLCRLGYCAYVPDR
                     SLSAKAGSGFCPRILTHSEVQQLLQAVDQLTPNARAPLRHLIMPEVFRLLYGCGFRVN
                     EVLHLRLGDVDLDRGGLTVRQAKFGKDRLVPPALPLVQRLQRYAASFGPRPSDAFFFP
                     "
     gene            complement(643..1908)
                     /locus_tag="AC159B_06110"
     CDS             complement(643..1908)
                     /locus_tag="AC159B_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HIR3377353.1"
                     /translation="MDSSAHRIPSSLPLDQLVAHALSELDKINYSRRSLRRYRTVWGH
                     LVTFSRERSLGDRYSEQLATEFVTAYHRHPGERVKANEEWRRHIPFLLKVLGDFARDG
                     RVERTRIDTSRLQVPAVMKKPLSDYEQYCRERRHLRASTLHGRMRTIAVFLDDLGSRN
                     VTTLDQLQPADIAAFVASRHRLGPRSMSRTVSDVRCFLQFLLLRGILPRDLSQMLPTV
                     RVPRDAAIPSVWEPELVERLLKVVERSSPRGKRDYAILLLACRLGLRLGDIRTLRLDD
                     LKWDTAAIEITQSKTGAPLCLPMTEEVGEALIDYLKSGRPQTAHRELFLRAQPPFTPF
                     ADDGHFYQIVKYWRELAGIRFRSKQHCGLHSLRHTLATQLLREQTPMHVISEILGHAT
                     TAATMIYAKADVESLRTAALDTEELRHGN"
     gene            complement(2037..2523)
                     /locus_tag="AC159B_06115"
                     /pseudo
     CDS             complement(2037..2523)
                     /locus_tag="AC159B_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          495 a          777 c          785 g          466 t
ORIGIN      
        1 gggaagaaga acgcatcgga cggacgcggt ccaaagctcg cggcgtacct ttgcagacgc
       61 tgcacgagcg gcaaggcggg cggaactagt cgatccttgc cgaacttggc ctggcgcacc
      121 gtcaagccac cccgatcgag atcgacatcg cccagccgca agtgcaagac ctcgttcacc
      181 cggaagccgc acccgtagag gaggcggaag acttcgggca tgataaggtg gcgcagcggc
      241 gcgcgtgcgt tcggcgtgag ttgatcaacc gcctgaagca gttgctgaac ctcgctgtgg
      301 gtcaggatgc gcggacagaa tccgctgcca gcctttgccg acagcgatcg gtctgggaca
      361 taggcgcagt agccgagccg gcacaggtag ccggcgaact ggcgcaccag cgagatacgg
      421 cactgctgcg tcctcgcgct ctcgtgggat tgcttggcga gccactgccg gacgagagct
      481 ctgggcaatt cacactgcag tagtccctcg gcgatgagat agcgatcaaa acatctgagt
      541 agccggatgc cctcgccgaa tcggtacccg catgcctgtt tcgtctgtac gaactgttcg
      601 atgagcgagg cgagcgggct ctggaaccgt ggttggagtt cgctaattgc catgacgcag
      661 ctcctcggta tccagtgcag cggtgcgcaa ggactccacg tcggctttgg cgtagatcat
      721 cgtcgccgct gtcgtggcgt gccccaggat ctccgagatg acatgcatcg gcgtttgctc
      781 gcgcagcagt tgcgtcgcca gcgtgtggcg cagcgagtgc agcccgcagt gctgtttgct
      841 ccggaaccga atgcccgcaa gttcccgcca atacttgacg atctgataga aatgaccgtc
      901 gtcggcgaac ggtgtgaaag gcggttgggc tctgaggaac agttcgcgat gggcggtctg
      961 ggggcggccc gacttcaggt agtcgatcag cgcctcgccg acttcctcgg tcatgggcaa
     1021 acacagcggc gcaccggtct tcgattgcgt gatctcgatg gcggccgtgt cccatttgag
     1081 atcgtcgagc ctgagcgttc ggatatcgcc caatcgcaat cccaggcgac atgcaagcag
     1141 gaggatggcg tagtcgcgct tgcctctggg ggagctgcgc tcgaccacct tcagaagcct
     1201 ctcaacgagt tccggctccc acaccgaagg gatcgcggca tcccgcggga cccggaccgt
     1261 cggcagcatc tggctgaggt cccgcggcag gattccgcgc agcagaagaa actgcaaaaa
     1321 acagcgcacg tcggaaacgg tccgggacat cgaccgggga cccagacgat gtcgcgaggc
     1381 gacgaatgcg gcgatatcgg ccggctgcaa ctgatccagc gtcgtcacgt tccttgaccc
     1441 caggtcatcc aggaataccg cgatcgttcg catccgcccg tgcagggtgg acgctcgaag
     1501 atggcgtcgc tcgcggcaat actgctcgta gtcgctcagc ggcttcttca tgaccgccgg
     1561 gacctgaagt ctcgacgtgt cgatgcgagt gcgctcgacg cggccgtcgc gggcaaagtc
     1621 gcccagcacc ttgaggagaa acgggatatg gcgccgccac tcctcgtttg ctttaacgcg
     1681 ttcacctggg tgtcggtggt acgcggttac gaactcagtc gccagctgct cggagtacct
     1741 gtctcccagg ctcctttcgc gtgagaacgt aacgagatgc ccccagaccg tgcggtaccg
     1801 cctcagcgat cgtctgctgt agttgatctt gtcgagctcg ctcaacgcgt gcgcaacaag
     1861 ttgatcaagc ggcaaactgg acgggattcg atgcgccgat gagtccatga ccaacctcct
     1921 tcgaaagagg cgaccacgat ggccgccggg aggctgatct tgaaacctta tgtgcagtga
     1981 tccaacgcta acgccttgtc cgcaaggtgc ttgcccgcgc tactgcacat aacaacttac
     2041 tgcccacaat aaatcatggt cgtggccaca tcggcttggc caagaagctc ttgcacgctg
     2101 cggatgtcat agccggcctg taacactgcc gttgcaaaag agtgtcgtaa gctgtgtggc
     2161 gtcgctggtt tagcaatgcc agtccgcaac aaggcgcgct tgaaagccct ctggaacgtt
     2221 tggtcgtaca gatggtgccg gcgcacgatg ccggagcgcg ggtccaccga atgcgtgtcc
     2281 tggggaaaca cccagaacca cgtccaggaa gttcccgcac gcgggtactt gcggtcaaga
     2341 gcatcaggca tggcgacgcc aacgcacccg gccgcccggt ccgcttgcca taacacccgc
     2401 gcccggccca gttgctcacg cagttgaggc gcgagtcggc tggggcagca tgaccgcccg
     2461 atcctttcct cccttgcctt cacgaacgat gatcgtgccg cgcccgaaat caatatcctt
     2521 tac
//
LOCUS       DBNGSB010000208         4535 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Kaftiobacteriaceae bacterium isolate
            MAG05310 SRR25158416_k127_151777, whole genome shotgun sequence.
ACCESSION   DBNGSB010000208 DBNGSB010000000
VERSION     DBNGSB010000208.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414718
            Sequence Read Archive: SRR25158416
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kaftiobacteriaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Kaftiobacteriaceae bacterium
            Bacteria; Bacillati; Candidatus Sysuimicrobiota; Candidatus
            Sysuimicrobiia; Candidatus Sysuimicrobiales; Candidatus
            Kaftiobacteriaceae.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:04:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,226
            CDSs (total)                      :: 1,204
            Genes (coding)                    :: 1,187
            CDSs (with protein)               :: 1,187
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 2 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Candidatus Kaftiobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158416_k127_151777"
                     /isolate="MAG05310"
                     /isolation_source="soil"
                     /db_xref="taxon:3693660"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="47.20055556 N 124.2113889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /gene="argC"
                     /locus_tag="AC159B_06120"
     CDS             complement(<1..554)
                     /gene="argC"
                     /locus_tag="AC159B_06120"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:NF013298.7"
                     /note="catalyzes the reduction of N-acetyl-5-glutamyl
                     phosphate to N-acetyl-L-glutamate 5-semialdehyde in
                     arginine biosynthesis and the reduction of
                     N-acetyl-gamma-aminoadipyl-phosphate to
                     N-acetyl-L-aminoadipate-semialdehyde in lysine
                     biosynthesis; involved in both the arginine and lysine
                     biosynthetic pathways; lysine is produced via the AAA
                     pathway, lysine from alpha-aminoadipate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HIR3377354.1"
                     /translation="MIKVSIIGASGYGGGELARLLVGHPEVELVHLTAESKAGDAMAD
                     LYPNLRSFTSAITEPADVAQIAQDSDVVFLALPNGKAMELAPGIAPHARLVDLGADFR
                     FKDSQVYEQWYKFPHVSPDLLADAAYGLTEFRRGEVRRANIVGNPGCYPTAALLATVP
                     LLQSDKVRKDGVIVDAKSGVSGAGR"
     gene            complement(712..3879)
                     /gene="carB"
                     /locus_tag="AC159B_06125"
                     /pseudo
     CDS             complement(712..3879)
                     /gene="carB"
                     /locus_tag="AC159B_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019005156.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
     gene            complement(3872..>4535)
                     /locus_tag="AC159B_06130"
     CDS             complement(3872..>4535)
                     /locus_tag="AC159B_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - L-glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HIR3377355.1"
                     /translation="ALDEQDLVGEVSRKEPLLIPGDGPRIALRDYGVKMGIVDALRRR
                     GCEVVLLPHTTHAEAILALDPAGVVLSNGPGDPAVLAAAVATVGDLVGRLPVMGVCLG
                     HQLLARSLGGRTYKMKFGHRGSNQPVLDQTSSRVMITTQNHGYAVDQESLPSSVEVTH
                     LNLNDGTVEGLRHRHLPAFSVQFHPEGRPGPTDADFLYDSFLALIKERSGTPSSYTKE
                     HA"
BASE COUNT          826 a         1490 c         1408 g          811 t
ORIGIN      
        1 cggcccgccc cggacacgcc ggatttggca tcgacgatga ccccgtcctt ccggaccttg
       61 tccgactgca gcaacggcac cgttgcaagc agcgcggcgg tcggataaca ccctggattt
      121 cctacgatgt tggcgcggcg cacttcgccg cgccggaatt ccgtcagacc ataggcagca
      181 tcggccagca ggtcagggct gacgtgggga aacttgtacc actgctcata cacctgtgag
      241 tctttgaacc ggaagtcggc accgaggtct accagccgcg catgcggggc aatccccggc
      301 gccagttcca ttgcctttcc gttgggcagc gcgagaaaga cgacgtcgga gtcttgtgcg
      361 atctgtgcga cgtccgcggg ctcggtaatc gcggacgtga aggagcgcag atttggatac
      421 agatcggcca tcgcgtcgcc ggccttgctc tccgcggtga ggtgaacgag ctcaacctcc
      481 ggatggccga ccagcaggcg cgccagctcg ccgccgccgt agcctgaggc tccgatgatg
      541 ctgaccttga tcatggatac gtataatcat accctatagg gtataaacat gcaacggcgg
      601 acttttcgaa agacagctgg aaagctggag agctaaagag ctggagagca gaagcacaaa
      661 gcgtcgtttt ccagctcttc agctttccag ctcttcagct ttctttggac gtcagatcgg
      721 ggtgggtccg gacagcggct ggacgtccgt gtcggatggc gacgcttcga gcaccgccgc
      781 gatgacgcgg gccgtgtcca gcgacgtgac gcacgggatc ctgcgatcga gcgcggcgcg
      841 ccggagccgg aatccgcggc gcgccgcgtc gcgtccgagg gttggggtgt tgatcacgag
      901 tccgaccctt ccggccgcga gcgcatcaaa accatcgacg gcatcggtgg gcatgccggc
      961 gtctcggagc gcggacgccg tgcctcctgt ggcgacaaca tggaagccca ggcgccggaa
     1021 cgattgcaga acagggtacg cgcggatgcg gtcgcgcggg gcgagtgaga ccagcaccgt
     1081 atcccccttc acctgcaacc ccgcggcgac gagcgcgcgg cgcaatgcgg cgggggcgtc
     1141 tgcgccgagc cccataacct ctcccgtgga cttcatttca ggacccaatg cagcttccgc
     1201 gcggatgagc ttctccatcg agaacaccgg tgccttgacg gcgacgcgcg gcggcgaggg
     1261 aagtcgatac actcccggat aacccaggtc gctgagacgg tcgccgatca tcacccgtgt
     1321 accgatctgg acgagtggga cgctcaccgc cttgctgacg aatggaacgg tccggctcgc
     1381 ccgcaggttc gcctcgagaa catacacccg cgtaccgtcg tagacgcatt ggagattgat
     1441 gaatccgcgc acacccagcc ctgccgcgat ctgactcacg atggcgatga tgcattcctc
     1501 gacaaaatcc ggaagcgact gcgccggaaa gatcgcgatg ctgtctcctg agtggattcc
     1561 cgcggcctcc acatgctcca taatgcccgg gatcaaaacc gactcgccgt cggcgatcgc
     1621 gtcgacctcg acctcgagac ctccgatgta gcgatcgatg agcaccggat actcttgatc
     1681 ggcggtgagc gcctcctgga ccgcggcgag cacagcggcc tcatcagcgg cgatctccat
     1741 gccccggccg ccgaggacgt acgatggacg caccaggacg gggaggccaa ggcgcctgac
     1801 gagggcgcgc gcttcttcga tcgtccgcac ggcgccgcct tgcggtcgct cgatgttcag
     1861 ttgcgcaagg aatgcgtcga actttcgccg gtcctcggag atgtccagtg atgtcacgga
     1921 cgtgccgagg accgccacgc ctgcggcacg cagtggacca gcaaggttga tcgccgtttg
     1981 tcctccgtac tgtaggagca cgccggttgg ctgctcccgt tcgcacacgt ggagcacatc
     2041 ctccagcgtc aggggttcga agtatagccg gtcagaaatg tcgaagtcgg tgctgacggt
     2101 ctctggattg ttgttgatga tgaccgcctc atagcccgcc tcgcgcagcg ccttgactgc
     2161 gtggaccgtt gaatagtcga actcgatccc ctgcccgatc cggatcggcc cggaccccag
     2221 caccacgatc cgcggcctgc ggaactcagg cgtttcatcc tcagtttcgt acgtcgagta
     2281 gtagtagggc gtctcggcgt ggaactcgcc ggcgcacgtg tccacgatct tgaacacagg
     2341 gcggatgccg gcgctgagcc ggcgattcct gacggtctct tcggtttcac cctggatcgc
     2401 tgcgatggcg gagtcagaga acccggcccg cttcgcctct cccagatcct gggtgcctgc
     2461 agacagacgc gactcgatat ccacaatacg cgcgattttc gcaatgaaga aacggtcgat
     2521 catcgtgccg gcgatgaggt catcgatcgt ccacccccgc ctgagcgcct ccgccaccgc
     2581 gaacaggcgc agatcgttgg gttcggtcac gagccgttcc agctcggcag cggaccactg
     2641 cgctcccgcc ggatgacgca tcccgtccag cttgagatcg agagaccgca ccgccttgag
     2701 cagcgcctgc tcgaacgtgc ggccgatggc catcacctcg cccgtggcct tcatctccgt
     2761 gcccagccgg cggtccatcg atggaaactt gtcaaacggc caccgtggga tttttgcgac
     2821 cacatagtcc aaagcaggtt cgaacgccgc gcgcgtcacg ccggtgacgc ggtttgggat
     2881 ctcatctagc ctgagcccca cggcgatctt cgccgctacg cgcgcaatcg ggtatcctgt
     2941 cgctttgctg gccagggccg aggaccgcga gacgcgcggg ttcacctcga tgacgtaata
     3001 cgcgtcgctc gtagggtcga gcgcaaactg gacgttgcag ccgccggcga tgcgcaggcc
     3061 gtcgatgata cgcaaggccg ccgtgcgcaa ccgttgatac tcccgatcag agagcgtcag
     3121 cgagggggcc accacgacac tgtctcccgt gtgcacaccc attgggtcga cgttctccat
     3181 attgcagacc gtgatggacg tgccggacgc gtcgcgcatc acttcatact cgatctcttt
     3241 ccatcccagc acactgcgct cgaccaggcc cggcccgatc gggctggcct gaatgccgcg
     3301 cgcggcaacc acggcaagca cgtcgggcgt tcttgcgatc ccgccgccgg tcccacccag
     3361 ggtgaaggcg ggccgcacga cgacgggata tctgaggccc gcggcgattt tctacgcatc
     3421 ctcgactcgc ttcgctgtgc ggctttcagg taccggctgg ccgagttcca gcatcgaagt
     3481 ccgaaaccga tcccgatcct cggcgttcag aatcgcctcg agcggcgtcc ccagcagctc
     3541 gacgccatag cgatggagcg ttccgctctg tgcgagggcc agcgcgaggt tcaatcccgt
     3601 ctgaccaccg agggtggcca ggatcccgtc cggccgctct gcgacgatga tcttttcgac
     3661 gaactccggc gtcagcggct cgacgtatac ccggtccgcg agctcgacgt cggtcatgat
     3721 ggttgctggg ttgctgttga ccagcacgac ctcgaggcct tcctcgctca gcgcgcggca
     3781 cgcctgcgtt ccggagtagt cgaactctgc ggcctggcca atgataatcg ggccggagcc
     3841 gatgacaagg accttccgga tgtcgtgtct cctaggcatg ctctttcgta tagcttgaag
     3901 gagttcccga acgctctttg atcaacgcga gaaacgaatc gtacagaaag tcagcgtcgg
     3961 tgggaccggg gcggccctcg ggatgaaact gcaccgagaa tgccggcaag tggcgatggc
     4021 gcagaccctc caccgtcccg tcgttgaggt tgaggtgcgt cacctcgact gaggagggaa
     4081 gagactcttg atcaacggca tagccatggt tctgcgtggt gatcatgacg cggctgctcg
     4141 tctgatccag caccggctgg ttgctgcccc ggtgaccgaa cttcatctta tacgtacgcc
     4201 ccccgagcga gcgagccagc agttggtgac ccaggcacac gcccatcacc ggcagccgtc
     4261 caacgaggtc tccgaccgtc gccacagcag cggcaagcac agccggatct cctgggccgt
     4321 tggacagcac gacccctgcc gggtccagtg ccaggatcgc ctccgcatgc gtcgtatgcg
     4381 ggagcaagac gacctcgcac ccgcgacgcc gcagggcgtc aacgatgccc atcttaaccc
     4441 cgtaatcccg gagggcgatg cgcggaccgt cccctggaat cagcagcggt tcttttcgag
     4501 acacctcgcc gacgagatct tgctcatcaa gcgcg
//
